The sole genus in the family Methanospirillaceae whose organisms are progressively motile by means of polar, tufted flagella. They have been isolated from sewage-sludge and pear waste digesters as well as marine and non-marine habitats.
A phylum of ARCHAEA comprising at least seven classes: Methanobacteria, Methanococci, Halobacteria (extreme halophiles), Archaeoglobi (sulfate-reducing species), Methanopyri, and the thermophiles: Thermoplasmata, and Thermococci.
An order of anaerobic, coccoid to rod-shaped methanogens, in the kingdom EURYARCHAEOTA. They are nonmotile, do not catabolize carbohydrates, proteinaceous material, or organic compounds other than formate or carbon monoxide, and are widely distributed in nature.
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
An order of anaerobic, highly specialized methanogens, in the kingdom EURYARCHAEOTA. Its organisms are nonmotile or motile, with cells occurring as coccoid bodies, pseudosarcina, or rods. Families include METHANOMICROBIACEAE, Methanocorpusculaceae, and Methanospirillaceae.
The technique of spraying a tissue specimen with a thin coat of a heavy metal such as platinum. The specimen is sprayed from an oblique angle, which results in the uneven deposition of the coating. The varying thicknesses create a shadow effect and give a three-dimensional appearance to the specimen.
The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)
A type of scanning probe microscopy in which a very sharp conducting needle is swept just a few angstroms above the surface of a sample. The tiny tunneling current that flows between the sample and the needle tip is measured, and from this are produced three-dimensional topographs. Due to the poor electron conductivity of most biological samples, thin metal coatings are deposited on the sample.
A group of PROTEOBACTERIA represented by morphologically diverse, anaerobic sulfidogens. Some members of this group are considered bacterial predators, having bacteriolytic properties.
Worthless, damaged, defective, superfluous or effluent material from industrial operations.
Derivatives of propionic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxyethane structure.
Derivatives of formic acids. Included under this heading are a broad variety of acid forms, salts, esters, and amides that are formed with a single carbon carboxy group.
The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.
Deoxyribonucleic acid that makes up the genetic material of archaea.
One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
Refuse liquid or waste matter carried off by sewers.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The relationships of groups of organisms as reflected by their genetic makeup.

Energetics of syntrophic propionate oxidation in defined batch and chemostat cocultures. (1/17)

Propionate consumption was studied in syntrophic batch and chemostat cocultures of Syntrophobacter fumaroxidans and Methanospirillum hungatei. The Gibbs free energy available for the H(2)-consuming methanogens was <-20 kJ mol of CH(4)(-1) and thus allowed the synthesis of 1/3 mol of ATP per reaction. The Gibbs free energy available for the propionate oxidizer, on the other hand, was usually >-10 kJ mol of propionate(-1). Nevertheless, the syntrophic coculture grew in the chemostat at steady-state rates of 0.04 to 0. 07 day(-1) and produced maximum biomass yields of 2.6 g mol of propionate(-1) and 7.6 g mol of CH(4)(-1) for S. fumaroxidans and M. hungatei, respectively. The energy efficiency for syntrophic growth of S. fumaroxidans, i.e., the biomass produced per unit of available Gibbs free energy was comparable to a theoretical growth yield of 5 to 12 g mol of ATP(-1). However, a lower growth efficiency was observed when sulfate served as an additional electron acceptor, suggesting inefficient energy conservation in the presence of sulfate. The maintenance Gibbs free energy determined from the maintenance coefficient of syntrophically grown S. fumaroxidans was surprisingly low (0.14 kJ h(-1) mol of biomass C(-1)) compared to the theoretical value. On the other hand, the Gibbs free-energy dissipation per mole of biomass C produced was much higher than expected. We conclude that the small Gibbs free energy available in many methanogenic environments is sufficient for syntrophic propionate oxidizers to survive on a Gibbs free energy that is much lower than that theoretically predicted.  (+info)

Metabolism of benzoate, cyclohex-1-ene carboxylate, and cyclohexane carboxylate by "Syntrophus aciditrophicus" strain SB in syntrophic association with H(2)-using microorganisms. (2/17)

The metabolism of benzoate, cyclohex-1-ene carboxylate, and cyclohexane carboxylate by "Syntrophus aciditrophicus" in cocultures with hydrogen-using microorganisms was studied. Cyclohexane carboxylate, cyclohex-1-ene carboxylate, pimelate, and glutarate (or their coenzyme A [CoA] derivatives) transiently accumulated during growth with benzoate. Identification was based on comparison of retention times and mass spectra of trimethylsilyl derivatives to the retention times and mass spectra of authentic chemical standards. (13)C nuclear magnetic resonance spectroscopy confirmed that cyclohexane carboxylate and cyclohex-1-ene carboxylate were produced from [ring-(13)C(6)]benzoate. None of the metabolites mentioned above was detected in non-substrate-amended or heat-killed controls. Cyclohexane carboxylic acid accumulated to a concentration of 260 microM, accounting for about 18% of the initial benzoate added. This compound was not detected in culture extracts of Rhodopseudomonas palustris grown phototrophically or Thauera aromatica grown under nitrate-reducing conditions. Cocultures of "S. aciditrophicus" and Methanospirillum hungatei readily metabolized cyclohexane carboxylate and cyclohex-1-ene carboxylate at a rate slightly faster than the rate of benzoate metabolism. In addition to cyclohexane carboxylate, pimelate, and glutarate, 2-hydroxycyclohexane carboxylate was detected in trace amounts in cocultures grown with cyclohex-1-ene carboxylate. Cyclohex-1-ene carboxylate, pimelate, and glutarate were detected in cocultures grown with cyclohexane carboxylate at levels similar to those found in benzoate-grown cocultures. Cell extracts of "S. aciditrophicus" grown in a coculture with Desulfovibrio sp. strain G11 with benzoate or in a pure culture with crotonate contained the following enzyme activities: an ATP-dependent benzoyl-CoA ligase, cyclohex-1-ene carboxyl-CoA hydratase, and 2-hydroxycyclohexane carboxyl-CoA dehydrogenase, as well as pimelyl-CoA dehydrogenase, glutaryl-CoA dehydrogenase, and the enzymes required for conversion of crotonyl-CoA to acetate. 2-Ketocyclohexane carboxyl-CoA hydrolase activity was detected in cell extracts of "S. aciditrophicus"-Desulfovibrio sp. strain G11 benzoate-grown cocultures but not in crotonate-grown pure cultures of "S. aciditrophicus". These results are consistent with the hypothesis that ring reduction during syntrophic benzoate metabolism involves a four- or six-electron reduction step and that once cyclohex-1-ene carboxyl-CoA is made, it is metabolized in a manner similar to that in R. palustris.  (+info)

Pathway of propionate oxidation by a syntrophic culture of Smithella propionica and Methanospirillum hungatei. (3/17)

The pathway of propionate conversion in a syntrophic coculture of Smithella propionica and Methanospirillum hungatei JF1 was investigated by (13)C-NMR spectroscopy. Cocultures produced acetate and butyrate from propionate. [3-(13)C]propionate was converted to [2-(13)C]acetate, with no [1-(13)C]acetate formed. Butyrate from [3-(13)C]propionate was labeled at the C2 and C4 positions in a ratio of about 1:1.5. Double-labeled propionate (2,3-(13)C) yielded not only double-labeled acetate but also single-labeled acetate at the C1 or C2 position. Most butyrate formed from [2,3-(13)C]propionate was also double labeled in either the C1 and C2 atoms or the C3 and C4 atoms in a ratio of about 1:1.5. Smaller amounts of single-labeled butyrate and other combinations were also produced. 1-(13)C-labeled propionate yielded both [1-(13)C]acetate and [2-(13)C]acetate. When (13)C-labeled bicarbonate was present, label was not incorporated into acetate, propionate, or butyrate. In each of the incubations described above, (13)C was never recovered in bicarbonate or methane. These results indicate that S. propionica does not degrade propionate via the methyl-malonyl-coenzyme A (CoA) pathway or any other of the known pathways, such as the acryloyl-CoA pathway or the reductive carboxylation pathway. Our results strongly suggest that propionate is dismutated to acetate and butyrate via a six-carbon intermediate.  (+info)

Propionate formation by Opitutus terrae in pure culture and in mixed culture with a hydrogenotrophic methanogen and implications for carbon fluxes in anoxic rice paddy soil. (4/17)

Propionate-forming bacteria seem to be abundant in anoxic rice paddy soil, but biogeochemical investigations show that propionate is not a correspondingly important intermediate in carbon flux in this system. Mixed cultures of Opitutus terrae strain PB90-1, a representative propionate-producing bacterium from rice paddy soil, and the hydrogenotrophic Methanospirillum hungatei strain SK maintained hydrogen partial pressures similar to those in the soil. The associated shift away from propionate formation observed in these cultures helps to reconcile the disparity between microbiological and biogeochemical studies.  (+info)

Biochemical evidence for formate transfer in syntrophic propionate-oxidizing cocultures of Syntrophobacter fumaroxidans and Methanospirillum hungatei. (5/17)

The hydrogenase and formate dehydrogenase levels in Syntrophobacter fumaroxidans and Methanospirillum hungatei were studied in syntrophic propionate-oxidizing cultures and compared to the levels in axenic cultures of both organisms. Cells grown syntrophically were separated from each other by Percoll gradient centrifugation. In S. fumaroxidans both formate dehydrogenase and hydrogenase levels were highest in cells which were grown syntrophically, while the formate-H(2) lyase activities were comparable under the conditions tested. In M. hungatei the formate dehydrogenase and formate-H(2) lyase levels were highest in cells grown syntrophically, while the hydrogenase levels in syntrophically grown cells were comparable to those in cells grown on formate. Reconstituted syntrophic cultures from axenic cultures immediately resumed syntrophic growth, and the calculated growth rates of these cultures were highest for cells which were inoculated from the axenic S. fumaroxidans cultures that exhibited the highest formate dehydrogenase activities. The results suggest that formate is the preferred electron carrier in syntrophic propionate-oxidizing cocultures of S. fumaroxidans and M. hungatei.  (+info)

Syntrophomonas erecta sp. nov., a novel anaerobe that syntrophically degrades short-chain fatty acids. (6/17)

Two novel anaerobes, strains GB4-38(T) and SB9-1, were isolated from an upflow anaerobic sludge blanket reactor for treating bean-curd farm wastewater and lotus field mud, respectively. The strains degraded straight-chain fatty acids with 4-8 carbon atoms in syntrophic association with methanogens and converted 1 mol butyrate into about 2 mol acetate and presumably 2 mol H(2). None of the branched-chain fatty acids tested could be degraded. Benzoate was not degraded. Fumarate, sulfate, thiosulfate, sulfur and nitrate did not serve as electron acceptors for butyrate degradation. In the absence of a methanogen partner, strain GB4-38(T) grew on crotonate in pure culture; the generation time was about 5 h at 37 degrees C. However, strain SB9-1 grew on butyrate plus pentenoate, but not crotonate, in pure culture and the generation time was 18 h at 37 degrees C. Cells of GB4-38(T) and SB9-1 were straight rods and stained Gram-negative. The major cellular fatty acids of GB4-38(T) were C(14 : 0) (29.74 %), C(16 : 0) (17.00 %), C(16 : 1)omega5c (16.63 %) and isoC(17 : 1) I (15.34 %). ll-Diaminopimelic acid existed in the cellular peptidoglycan. The genomic DNA G+C content of strain GB4-38(T) was 43.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences supported clustering of the two strains with syntrophic bacterial species of the genus Syntrophomonas (89.6-92.4 % sequence similarity), but phenotypic, chemotaxonomic and genetic characters differentiated the two strains from members of this genus. Therefore, it is proposed that the two strains are representatives of a novel species, Syntrophomonas erecta sp. nov. The type strain is GB4-38(T) (=CGMCC 1.5013(T)=DSM 16215(T)).  (+info)

The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum. (7/17)

A Gram-positive, spore-forming, syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov. strain HH(T), was isolated as a co-culture with Methanospirillum hungatei JF-1(T) from anaerobic, freeze-dried granular sludge obtained from an upflow anaerobic sludge bed reactor treating sugar beet wastewater. The bacterium converted propionate to acetate in co-culture with Methanospirillum hungatei JF-1(T) or Methanobacterium formicicum MF(NT), but not in co-culture with Methanobrevibacter arboriphilus AZ. The organism could not be cultured axenically with any of the substrates tested and therefore can be considered as a (the first) true anaerobic syntrophic bacterium. The bacterium contained two distinct 16S rRNA gene sequences, with 96.8% sequence similarity, which were both expressed during syntrophic growth on propionate as revealed by fluorescent in situ hybridization. The most closely related organisms are Cryptanaerobacter phenolicus LR7.2(T), a bacterium that transforms phenol into benzoate, and Pelotomaculum thermopropionicum SI(T), a thermophilic, syntrophic propionate-oxidizing bacterium. Other related species belong to the Gram-positive, sulfate-reducing genus Desulfotomaculum. The type strain of Pelotomaculum schinkii is strain HH(T) (=ATCC BAA-615(T)=DSM 15200(T)).  (+info)

Pelotomaculum propionicicum sp. nov., an anaerobic, mesophilic, obligately syntrophic, propionate-oxidizing bacterium. (8/17)

An anaerobic, mesophilic, syntrophic, propionate-oxidizing bacterium, strain MGP(T), was isolated as a defined co-culture with Methanospirillum hungatei from the methanogenic sludge of a mesophilic upflow anaerobic sludge blanket (UASB) reactor. The strain grew in the presence of propionate, but only in co-culture with methanogens, suggesting that it is an obligately syntrophic bacterium. The optimum temperature for growth was 37 degrees C, and the optimum pH was between 6.5 and 7.2. Based on comparative 16S rRNA gene sequence analysis, strain MGP(T) was affiliated with subcluster Ih of 'Desulfotomaculum cluster I', in which it was found to be moderately related to known species of the genera Pelotomaculum and Cryptanaerobacter. Similar to known species of the genus Pelotomaculum, strain MGP(T) could degrade propionate in syntrophy, but had no ability to reduce sulfate, sulfite and thiosulfate. Further phenotypic and genetic studies supported the affiliation of the strain as a novel species in this genus, for which the name Pelotomaculum propionicicum sp. nov. is proposed. The type strain is MGP(T) (=DSM 15578(T)=JCM 11929(T)). The strain has been deposited in the DSM and JCM culture collections as a defined co-culture with Methanospirillum hungatei.  (+info)

"Methanospirillum" is a genus of archaea that belongs to the order Methanosarcinales and the family Methanosarcinaceae. These microorganisms are methanogens, which means they are capable of producing methane as a metabolic byproduct. They are typically found in anaerobic environments, such as sediments, waterlogged soils, and the digestive tracts of animals.

The cells of "Methanospirillum" species are long and slender, with a spiral or curved shape, and they can exist either individually or in pairs. They are able to grow autotrophically, using carbon dioxide as their carbon source, and they obtain energy by reducing methanol, methylamines, or acetate to methane.

It's important to note that "Methanospirillum" is a specific genus of archaea, and there are other genera of methanogens that exist as well. Each genus has its own distinct characteristics and metabolic capabilities.

Euryarchaeota is a phylum within the domain Archaea, which consists of a diverse group of microorganisms that are commonly found in various environments such as soil, oceans, and the digestive tracts of animals. This group includes methanogens, which are archaea that produce methane as a metabolic byproduct, and extreme halophiles, which are archaea that thrive in highly saline environments.

The name Euryarchaeota comes from the Greek words "eury," meaning wide or broad, and "archaios," meaning ancient or primitive. This name reflects the phylum's diverse range of habitats and metabolic capabilities.

Euryarchaeota are characterized by their unique archaeal-type cell walls, which contain a variety of complex polysaccharides and proteins. They also have a distinct type of intracellular membrane called the archaellum, which is involved in motility. Additionally, Euryarchaeota have a unique genetic code that differs from that of bacteria and eukaryotes, with some codons specifying different amino acids.

Overall, Euryarchaeota are an important group of archaea that play a significant role in global carbon and nitrogen cycles, as well as in the breakdown of organic matter in various environments.

Methanobacteriales is an order of methanogenic archaea within the kingdom Euryarchaeota. These organisms are characterized by their ability to produce methane as a metabolic byproduct in anaerobic environments. They are commonly found in habitats such as wetlands, digestive tracts of animals, and sewage sludge. The cells of Methanobacteriales are typically rod-shaped and have a Gram-positive stain, although they lack a true cell wall. Some notable genera within this order include Methanobrevibacter, Methanothermobacter, and Methanosphaera.

Negative staining is a histological or microscopy technique used to enhance the contrast of transparent or translucent specimens, such as bacteria and viruses. This technique involves applying a thin layer of a dense, dark-staining material (such as a heavy metal salt) onto the surface of the sample. The stain does not penetrate the specimen but rather forms a thin layer around it, creating a "negative" image where the specimen appears lighter against the dark background. This method is particularly useful for visualizing the shape and structure of small or delicate biological samples that would be difficult to see using other staining techniques.

Methanomicrobiales is an order of archaea within the methanogens, which are microorganisms that produce methane as a metabolic byproduct in anaerobic conditions. Members of Methanomicrobiales are characterized by their ability to produce methane through the reduction of carbon dioxide with hydrogen. They are commonly found in environments such as wetlands, digestive tracts of animals, and sewage sludge. The cells of Methanomicrobiales are typically irregularly shaped and do not form spores. Some notable families within this order include Methanocorpusculaceae, Methanogranolicaceae, and Methanospirillaceae.

Methane is not a medical term, but it is a chemical compound that is often mentioned in the context of medicine and health. Medically, methane is significant because it is one of the gases produced by anaerobic microorganisms during the breakdown of organic matter in the gut, leading to conditions such as bloating, cramping, and diarrhea. Excessive production of methane can also be a symptom of certain digestive disorders like irritable bowel syndrome (IBS) and small intestinal bacterial overgrowth (SIBO).

In broader terms, methane is a colorless, odorless gas that is the primary component of natural gas. It is produced naturally by the decomposition of organic matter in anaerobic conditions, such as in landfills, wetlands, and the digestive tracts of animals like cows and humans. Methane is also a potent greenhouse gas with a global warming potential 25 times greater than carbon dioxide over a 100-year time frame.

Scanning Tunneling Microscopy (STM) is not strictly a medical definition, but it is a type of microscopy used in some areas of scientific research including biophysics and nanotechnology. However, I can provide a general definition:

Scanning Tunneling Microscopy (STM) is a type of microscopy that uses quantum tunneling to form an image of a sample surface at the atomic level. In this process, a sharp conductive tip is brought very close to the surface of the sample, and a voltage bias is applied between the two. Electrons can tunnel through the vacuum gap between the tip and the sample, and the resulting current is measured and used to form an image of the surface topography with atomic resolution. It's worth noting that STM is not commonly used in medical diagnosis or treatment, but it has been used in some research applications related to medicine, such as studying the structure of individual molecules or investigating the properties of biomaterials at the nanoscale.

Deltaproteobacteria is a class of proteobacteria, which are a group of gram-negative bacteria. Deltaproteobacteria are characterized by their unique arrangement of flagella and their ability to perform anaerobic respiration, which means they can grow without oxygen. They play important roles in various environments such as soil, freshwater, and marine ecosystems, where they are involved in processes like sulfur cycling and denitrification. Some members of this class are also known to cause diseases in humans, such as the genera Myxococcus, Bdellovibrio, and Desulfovibrio.

I'm sorry for any confusion, but "industrial waste" is not a term that has a medical definition. Industrial waste is a term used to describe waste produced by industrial activities, such as manufacturing, mining, and construction. It can include a wide variety of materials, including chemicals, metals, soil, and water. Proper management and disposal of industrial waste are important for protecting the environment and public health. If you have any questions related to healthcare or medical terminology, I'd be happy to try to help answer those!

Propionates, in a medical context, most commonly refer to a group of medications that are used as topical creams or gels to treat fungal infections of the skin. Propionic acid and its salts, such as propionate, are the active ingredients in these medications. They work by inhibiting the growth of fungi, which causes the infection. Common examples of propionate-containing medications include creams used to treat athlete's foot, ringworm, and jock itch.

It is important to note that there are many different types of medications and compounds that contain the word "propionate" in their name, as it refers to a specific chemical structure. However, in a medical context, it most commonly refers to antifungal creams or gels.

I'm sorry for any confusion, but "Formates" is not a recognized term in medical terminology. It is possible that you may be referring to "formic acid" or "formate," which are terms used in chemistry and biochemistry. Formic acid is a colorless, pungent, and corrosive liquid with the chemical formula HCOOH. Its salts are called formates.

Formate is the anion (negatively charged ion) of formic acid, with the chemical formula HCOO-. Formate can be found in various biological systems and is involved in several metabolic processes. If you could provide more context or clarify your question, I would be happy to help further.

Fluid waste disposal in a medical context refers to the proper and safe management of liquid byproducts generated during medical procedures, patient care, or research. These fluids can include bodily excretions (such as urine, feces, or vomit), irrigation solutions, blood, or other biological fluids.

The process of fluid waste disposal involves several steps:

1. Collection: Fluid waste is collected in appropriate containers that are designed to prevent leakage and contamination.
2. Segregation: Different types of fluid waste may require separate collection and disposal methods based on their infectious or hazardous nature.
3. Treatment: Depending on the type and volume of fluid waste, various treatments can be applied, such as disinfection, sterilization, or chemical neutralization, to reduce the risk of infection or harm to the environment and personnel.
4. Disposal: Treated fluid waste is then disposed of according to local regulations, which may involve transporting it to a designated waste management facility for further processing or disposal in a safe and environmentally friendly manner (e.g., deep well injection, incineration, or landfilling).
5. Documentation and tracking: Proper records should be maintained to ensure compliance with regulatory requirements and to enable effective monitoring and auditing of the waste disposal process.

It is essential to handle fluid waste disposal carefully to minimize the risk of infection, protect the environment, and maintain regulatory compliance. Healthcare facilities must adhere to strict guidelines and regulations regarding fluid waste management to ensure the safety of patients, staff, and the community.

Archaeal DNA refers to the genetic material present in archaea, a domain of single-celled microorganisms lacking a nucleus. Like bacteria, archaea have a single circular chromosome that contains their genetic information. However, archaeal DNA is significantly different from bacterial and eukaryotic DNA in terms of its structure and composition.

Archaeal DNA is characterized by the presence of unique modifications such as methylation patterns, which help distinguish it from other types of DNA. Additionally, archaea have a distinct set of genes involved in DNA replication, repair, and recombination, many of which are more similar to those found in eukaryotes than bacteria.

One notable feature of archaeal DNA is its resistance to environmental stressors such as extreme temperatures, pH levels, and salt concentrations. This allows archaea to thrive in some of the most inhospitable environments on Earth, including hydrothermal vents, acidic hot springs, and highly saline lakes.

Overall, the study of archaeal DNA has provided valuable insights into the evolutionary history of life on Earth and the unique adaptations that allow these organisms to survive in extreme conditions.

Archaea are a domain of single-celled microorganisms that lack membrane-bound nuclei and other organelles. They are characterized by the unique structure of their cell walls, membranes, and ribosomes. Archaea were originally classified as bacteria, but they differ from bacteria in several key ways, including their genetic material and metabolic processes.

Archaea can be found in a wide range of environments, including some of the most extreme habitats on Earth, such as hot springs, deep-sea vents, and highly saline lakes. Some species of Archaea are able to survive in the absence of oxygen, while others require oxygen to live.

Archaea play important roles in global nutrient cycles, including the nitrogen cycle and the carbon cycle. They are also being studied for their potential role in industrial processes, such as the production of biofuels and the treatment of wastewater.

A bioreactor is a device or system that supports and controls the conditions necessary for biological organisms, cells, or tissues to grow and perform their specific functions. It provides a controlled environment with appropriate temperature, pH, nutrients, and other factors required for the desired biological process to occur. Bioreactors are widely used in various fields such as biotechnology, pharmaceuticals, agriculture, and environmental science for applications like production of therapeutic proteins, vaccines, biofuels, enzymes, and wastewater treatment.

Anaerobiosis is a state in which an organism or a portion of an organism is able to live and grow in the absence of molecular oxygen (O2). In biological contexts, "anaerobe" refers to any organism that does not require oxygen for growth, and "aerobe" refers to an organism that does require oxygen for growth.

There are two types of anaerobes: obligate anaerobes, which cannot tolerate the presence of oxygen and will die if exposed to it; and facultative anaerobes, which can grow with or without oxygen but prefer to grow in its absence. Some organisms are able to switch between aerobic and anaerobic metabolism depending on the availability of oxygen, a process known as "facultative anaerobiosis."

Anaerobic respiration is a type of metabolic process that occurs in the absence of molecular oxygen. In this process, organisms use alternative electron acceptors other than oxygen to generate energy through the transfer of electrons during cellular respiration. Examples of alternative electron acceptors include nitrate, sulfate, and carbon dioxide.

Anaerobic metabolism is less efficient than aerobic metabolism in terms of energy production, but it allows organisms to survive in environments where oxygen is not available or is toxic. Anaerobic bacteria are important decomposers in many ecosystems, breaking down organic matter and releasing nutrients back into the environment. In the human body, anaerobic bacteria can cause infections and other health problems if they proliferate in areas with low oxygen levels, such as the mouth, intestines, or deep tissue wounds.

I'm sorry for any confusion, but "Hydrogen" is not a medical term. It is a chemical element with the symbol H and atomic number 1. It is the lightest and most abundant chemical element in the universe, making up about 75% of its elemental mass.

In a medical context, hydrogen can be discussed in terms of molecular hydrogen (H2) which has been studied for potential therapeutic benefits. Some research explores its use as an antioxidant and anti-inflammatory agent, but more studies are needed to confirm these effects and understand the mechanisms behind them.

Sewage is not typically considered a medical term, but it does have relevance to public health and medicine. Sewage is the wastewater that is produced by households and industries, which contains a variety of contaminants including human waste, chemicals, and other pollutants. It can contain various pathogens such as bacteria, viruses, and parasites, which can cause diseases in humans if they come into contact with it or consume contaminated food or water. Therefore, the proper treatment and disposal of sewage is essential to prevent the spread of infectious diseases and protect public health.

Ribosomal RNA (rRNA) is a type of RNA that combines with proteins to form ribosomes, which are complex structures inside cells where protein synthesis occurs. The "16S" refers to the sedimentation coefficient of the rRNA molecule, which is a measure of its size and shape. In particular, 16S rRNA is a component of the smaller subunit of the prokaryotic ribosome (found in bacteria and archaea), and is often used as a molecular marker for identifying and classifying these organisms due to its relative stability and conservation among species. The sequence of 16S rRNA can be compared across different species to determine their evolutionary relationships and taxonomic positions.

Ribosomal DNA (rDNA) refers to the specific regions of DNA in a cell that contain the genes for ribosomal RNA (rRNA). Ribosomes are complex structures composed of proteins and rRNA, which play a crucial role in protein synthesis by translating messenger RNA (mRNA) into proteins.

In humans, there are four types of rRNA molecules: 18S, 5.8S, 28S, and 5S. These rRNAs are encoded by multiple copies of rDNA genes that are organized in clusters on specific chromosomes. In humans, the majority of rDNA genes are located on the short arms of acrocentric chromosomes 13, 14, 15, 21, and 22.

Each cluster of rDNA genes contains both transcribed and non-transcribed spacer regions. The transcribed regions contain the genes for the four types of rRNA, while the non-transcribed spacers contain regulatory elements that control the transcription of the rRNA genes.

The number of rDNA copies varies between species and even within individuals of the same species. The copy number can also change during development and in response to environmental factors. Variations in rDNA copy number have been associated with various diseases, including cancer and neurological disorders.

Electron microscopy (EM) is a type of microscopy that uses a beam of electrons to create an image of the sample being examined, resulting in much higher magnification and resolution than light microscopy. There are several types of electron microscopy, including transmission electron microscopy (TEM), scanning electron microscopy (SEM), and reflection electron microscopy (REM).

In TEM, a beam of electrons is transmitted through a thin slice of the sample, and the electrons that pass through the sample are focused to form an image. This technique can provide detailed information about the internal structure of cells, viruses, and other biological specimens, as well as the composition and structure of materials at the atomic level.

In SEM, a beam of electrons is scanned across the surface of the sample, and the electrons that are scattered back from the surface are detected to create an image. This technique can provide information about the topography and composition of surfaces, as well as the structure of materials at the microscopic level.

REM is a variation of SEM in which the beam of electrons is reflected off the surface of the sample, rather than scattered back from it. This technique can provide information about the surface chemistry and composition of materials.

Electron microscopy has a wide range of applications in biology, medicine, and materials science, including the study of cellular structure and function, disease diagnosis, and the development of new materials and technologies.

Phylogeny is the evolutionary history and relationship among biological entities, such as species or genes, based on their shared characteristics. In other words, it refers to the branching pattern of evolution that shows how various organisms have descended from a common ancestor over time. Phylogenetic analysis involves constructing a tree-like diagram called a phylogenetic tree, which depicts the inferred evolutionary relationships among organisms or genes based on molecular sequence data or other types of characters. This information is crucial for understanding the diversity and distribution of life on Earth, as well as for studying the emergence and spread of diseases.

2002 Methanospirillum Ferry, Smith & Wolfe 1974 Family Methanoregulaceae Sakai et al. 2012 Methanolinea Imachi et al. 2008 ...
"Pathway of Propionate Oxidation by a Syntrophic Culture of Smithella propionica and Methanospirillum hungatei". Applied and ...
... for Formate Transfer in Syntrophic Propionate-Oxidizing Cocultures of Syntrophobacter fumaroxidans and Methanospirillum ...
It is able to be cultured syntrophically with Methanospirillum hungatei, where it produces methane from glucose in appropriate ...
These scavengers, like Methanospirillum and Acetobacterium, metabolize the H2 waste produced during amino acid breakdown, ...
See image at right). In archaeal PRK of Methanospirillum hungatei, PRK (or MhPRK) exists as a homodimer of two protomers, each ... only two crystal structures have been resolved for this class of enzymes in Rhodobacter sphaeroides and Methanospirillum ...
... methanospirillum MeSH B07.200.765.550 - methanosarcinaceae MeSH B07.200.765.550.550 - methanosarcina MeSH B07.200.765.550. ...
Methanospirillum hungatei, at the air/water interface. Tomoaia-Cotisel M, Chifu E, Zsako J, Mocanu A, Quinn PJ, Kates M. Chem ...
ISBN 978-0-387-98771-2. PubMed references for Methanospirillum PubMed Central references for Methanospirillum Google Scholar ... references for Methanospirillum NCBI taxonomy page for Methanospirillum Search Tree of Life taxonomy pages for Methanospirillum ... Search Species2000 page for Methanospirillum MicrobeWiki page for Methanospirillum LPSN page for Methanospirillum v t e ( ... In taxonomy, Methanospirillum is a genus of microbes within the family Methanospirillaceae. All its species are methanogenic ...
This family contains only one genus, Methanospirillum. All its species are methanogenic archaea. The cells are bar-shaped and ...
Poweleit N, Ge P, Nguyen HH, Loo RR, Gunsalus RP, Zhou ZH (December 2016). "CryoEM structure of the Methanospirillum hungatei ...
ISBN 978-0-387-98771-2. PubMed references for Methanospirillum PubMed Central references for Methanospirillum Google Scholar ... references for Methanospirillum NCBI taxonomy page for Methanospirillum Search Tree of Life taxonomy pages for Methanospirillum ... Search Species2000 page for Methanospirillum MicrobeWiki page for Methanospirillum LPSN page for Methanospirillum v t e ( ... In taxonomy, Methanospirillum is a genus of microbes within the family Methanospirillaceae. All its species are methanogenic ...
Methanospirillum hungatei JF1 as the outgroup. A total of 280 sequences were used to construct a neighbor-joining phylogenetic ...
Hydrogenotrophic Methanospirillum species were the dominant methanogens in all the SCG digestion tests. It is likely that NaOH ... Methanospirillum stamsii strain ps 16S. 98.2. NR_117705. Methanospirillum. Methanospirillum lacunae strain Ki8-1. 98.2. NR_ ... Methanospirillum hungatei strain JF-1. 97.8. CP000254. Methanospirillum. Methanospirillum lacunae strain Ki8-1. 97.8. NR_112981 ... Both bands were closely related (≥97% sequence similarity) to several Methanospirillum species (Table 6). Methanogens belonging ...
We also identified, purified, and characterized the major flagella protein from archaeon Methanospirillum hungatei JF1. We ... Methanospirillum hungatei. For one of these, S. wolfei, the core pathways for carbon and electron flow is depicted in Figure 1A ... previously undescribed surface layer proteins in Methanospirillum hungatgei (Figure 2A and manuscripts in preparation), and ...
Methanospirillum hungatei JF-1 Archaea normal 0.0326903 normal 0.301523 -. NC_007355 Mbar_A3575 putative surface layer protein ... Methanospirillum hungatei JF-1 Archaea hitchhiker 0.0000335997 normal 0.167692 -. NC_007355 Mbar_A3737 hypothetical protein ...
Host Lineage: Methanospirillum hungatei; Methanospirillum; Methanospirillaceae; Methanomicrobiales; Euryarchaeota; Archaea. ... Query: NC_007796:1731500:1758673 Methanospirillum hungatei JF-1, complete genome. Start: 1758673, End: 1759422, Length: 750. ...
Methanothrix and Methanospirillum). Correspondingly, the metabolic functions of membrane transport, substrate metabolism, ...
... and emended descriptions of the genus Methanospirillum and Methanospirillum hungatei. T Iino, K Mori, K Suzuki ... Methanospirillum lacunae sp. nov., a methane-producing archaeon isolated from a puddly soil, ...
Methanospirillum hungatei JF-1 Archaea normal 0.579359 normal 0.139276 -. NC_009092 Shew_3744 peptidase M6, immune inhibitor A ... Methanospirillum hungatei JF-1 Archaea normal 0.217586 normal 1 -. NC_007954 Sden_2272 endonuclease/exonuclease/phosphatase ...
Methanospirillum hungatei JF-1. Methanothermobacter thermautotrophicus str. Delta H. Methanothermus fervidus. Moloney murine ...
Methanospirillum hungatei JF-1 Position: -182. Score: 5.01997. Sequence: TACACTAACTTCAAGTAAATACT Locus tag: Mhun_0129. Name: ...
Methanospirillum, Methanobrevibacter and unassigned Methanomassilococeae). Methanosaeta; the archetypal acetoclastic methanogen ...
RL18_METPE Methanospirillaceae Methanospirillum Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100... ( ... RL18_METHJ Methanospirillum lacunae (UP000245657) A0A2V2MUE4_9EURY Methanophagales Methanophagales archaeon (UP000247916) ...
The Archaellum of Methanospirillum hungatei Is Electrically Conductive.. mBio. 10(2)*PubMed ...
Relating mRNA and protein biomarker levels in a Dehalococcoides and Methanospirillum-containing community Journal Article ...
I decided to focus on C. botulinum B (the nasty food-poisoning organism) and the archeon Methanospirillum hungatei strain JF-1 ... Methanospirillum (a strict anaerobe found in sewage) produces colonies with characteristic striations spaced two cell-lengths ...
Methanospirillum hungatei or Acetobacterium woodii as a partner, glycolic acid is converted to carbon dioxide and hydrogen [3]. ...
Methanospirillum, Methanothermobacter, Methanothermus, and Methanothermus metabolise hydrogen to produce methane biogas. ...
Methanospirillum Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100... (UP000001941) Methanospirillum ...
... for methanogenesis as suggested by the annotated sequences associated with genera fantofarone such as Methanospirillum ...
Methanospirillum Respiratory mRNA Biomarkers Correlate with Hydrogenotrophic Methanogenesis Rate during Growth and Competition ...
Flap endonuclease 1 OS=Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 / JF-1) GN=fen PE=3 SV=1. 1. ...
A number of microbes, including Alcaligenes, Cellulosimicrobium, Microbacterium, Micrococcus, Methanospirillum, Aeromonas, ...
... the total population of microorganisms and is dominated by hydrogentrophic methanogens belonging to the genera Methanospirillum ... the total population of microorganisms and is dominated by hydrogentrophic methanogens belonging to the genera Methanospirillum ... población de microorganismos y está dominada por metanogénicos hidrogenotróficos pertenecientes a los géneros Methanospirillum ...
"The research group discovered that Methanospirillum hungatei, a microbe (methanogenic archaeon) which is thought to have ...
011449470 394 EGCRTLINLINQWGS 408 Methanospirillum hungatei WP_010870355 398 EAFEELIGMINFFGM 412 Methanocaldococcus jannaschii ...
Methanococcus maripaludis and Methanospirillum hungatei, at different growth rates. Comparative whole-genome transcriptional ...
  • The Archaellum of Methanospirillum hungatei Is Electrically Conductive. (umass.edu)
  • I decided to focus on C. botulinum B (the nasty food-poisoning organism) and the archeon Methanospirillum hungatei strain JF-1 , which is named after a professor I had in grad school, Bob Hungate, a true pioneer in the study of anaerobic bacteria. (blogspot.com)
  • The maximum response of SD (36.4%) was obtained at 0.18 M NaOH and 90.0 °C, while that of Ym (263.31 mL CH 4 /g COD added) was obtained at 0.13 M NaOH and 70.5 °C. Hydrogenotrophic Methanospirillum species were the dominant methanogens in all the SCG digestion tests. (mdpi.com)
  • The qPCR data, using universal 16S rRNA primers of Eubacteria and Archaea, suggest that the methanogenic population comprises nearly is 25 % of the total population of microorganisms and is dominated by hydrogentrophic methanogens belonging to the genera Methanospirillum and Methanobacterium. (uprm.edu)
  • Methanospirillum (a strict anaerobe found in sewage) produces colonies with characteristic striations spaced two cell-lengths apart. (blogspot.com)
  • In taxonomy, Methanospirillum is a genus of microbes within the family Methanospirillaceae. (wikipedia.org)
  • Os gêneros mais abundantes identificados na Fase V foram Anaeromusa-Anaeroarcus , Lactivibrio e Synergistaceae , para o Domínio Bacteria, e Methanosaeta , Methanospirillum e Methanobacterium , para o Domínio Archaea. (usp.br)
  • Methanospirillum, a new genus of methanogenic bacteria, and characterization of Methanospirillum hungatii sp. (wikipedia.org)
  • Family, in the order METHANOMICROBIALES , which contains only one genus METHANOSPIRILLUM . (nih.gov)