Aequorin

Luminescent Measurements

Luminescent Proteins

COVID-19 testing

Marc Zimmer

Nanomedicine

Lanthanide probes

Protein tag

Stern-Volmer relationship

Aequorin

Outline of photography

Thalappil Pradeep

Renilla-luciferin 2-monooxygenase

Tetrasodium tris(bathophenanthroline disulfonate)ruthenium(II)

Lactoferrin

Fluorescence in the life sciences

Reporter gene

Green fluorescent protein

Lomonosov Gold Medal

Amyloid plaques

Pseudotyping

Calmodulin

Photoprotein

Antibiotic sensitivity testing

Anthony Campbell (British biochemist)

Coelenterazine

Gerd Ulrich Nienhaus

Bioluminescence imaging

Cuttlefish

Streptavidin

Apoptosis

Eos (protein)

Biofilm

Types of luminescent2

• For the first time, Manganese complexes show the types of luminescent properties and photocatalytic behavior that were primarily associated with noble metal compounds until now. (phys.org)
• In this thesis, we applied the 2D POLIM technique to investigate the fundamental optoelectronic process in different types of luminescent materials. (lu.se)

Genetic2

• These vectors are designed for protein transient expression and genetic transformation. (jove.com)
• Most cases of human prion disease are apparently spontaneously occurring (sporadic CJD [sCJD]) or are associated with mutations in the human prion protein gene, designated PRNP (genetic CJD, Gerstmann-Sträussler-Scheinker disease, or fatal familial insomnia). (cdc.gov)

Fluorescence2

• The ligand bTVBT4 shows specificity for detection of tau proteins in the Alzheimer's fold by means of fluorescence imaging. (e-science.se)
• Our extensive SpectraMax® line of user-friendly microplate readers - the industry's most cited brand - provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation and include absorbance, fluorescence, and luminescence. (moleculardevices.com)

Assay2

• To demonstrate its versatility, we implemented and validated the assay in vitro and in vivo for the bacterial Sec system and the mitochondrial protein import apparatus. (uni-luebeck.de)
• We used a rapid molecular conversion assay (protein misfolding cyclic amplification) to test whether brain homogenates from specimens of classical bovine spongiform encephalopathy (BSE), atypical BSE (H-type BSE and L-type BSE), classical scrapie, atypical scrapie, and chronic wasting disease can convert normal human prion protein to the abnormal disease-associated form. (cdc.gov)

Bacteria2

• Structure of lumazine protein, an optical transponder of luminescent bacteria. (mpg.de)
• E. coli already has a sensor to detect TNT, which it can metabolize and use as fuel, but the university lab was able to change the nature of the bacterium "so instead of having the bacteria just break down TNT, it activates a luminescent protein" that was derived from a different organism, he said. (nationaldefensemagazine.org)

LUMINESCENCE1

• While luminescence has become more established in biological research, the green fluorescent protein (GFP) behind the glow was first identified in Aequorea victoria . (buzzfeednews.com)

Genes1

• Although HMG proteins do not possess intrinsic transcriptional activity, they are called architectural transcription factors because they modulate the transcription of their target genes by altering the chromatin structure at the promoter and/or enhancers 15 . (nature.com)

Biological4

• Therefore, this chapter will show exciting results of the group using fluorescent proteins and nanocrystals in biological systems. (intechopen.com)
• After publishing Aglow in the Dark in 2005, Gruber moved to Brown University for postdoctoral study on ways to use fluorescent proteins as biological sensors. (the-scientist.com)
• Protein is one of the central biomolecules that is involved in almost all biological events and thus its quantitative analysis has been actively conducted for many years. (nii.ac.jp)
• 2D POLIM was also applied to explore the aggregation state of proteins in the biological system. (lu.se)

Specificity1

• Fluorescent reporter proteins in the Drosophila model system offer a degree of specificity that allows monitoring cellular and biochemical phenomena in vivo, such as autophagy, mitophagy, and changes in the redox state of cells. (intechopen.com)

Inhibitors1

• After transfecting for 24 hr, cells were lysed by RIPA buffer including protease inhibitors, and biotinylated proteins were pulled down with streptavidin beads. (elifesciences.org)

Fluorescent protein4

• An HIV vector containing a gene encoding the green fluorescent protein (GFP) was injected into the subretinal space of rat eyes. (nih.gov)
• Few scientists knew the complete history of fluorescent protein research, so he and Pieribone wrote a book about it. (the-scientist.com)
• This study shows the CLD of RanBP2 promotes selectively the accumulation of a subset of reporter substrates of the UPS, such as the ubiquitin (Ub)-fusion yellow fluorescent protein (YFP) degradation substrate, Ub(G76V)-YFP, and the N-end rule substrate, Ub-R-YFP. (duke.edu)
• In this study, we genetically modified P. falciparum to simultaneously express GFP, a fluorescent protein, and NanoLuc, a luciferase. (nagasaki-u.ac.jp)

Luciferase2

• To address this, we applied NanoLuc split-luciferase to produce a new strategy for measuring protein transport. (uni-luebeck.de)
• Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)

Kinase3

• We have previously shown that HMGA2-induced transcription requires phosphorylation of H2A.X at S139, which in turn is mediated by the protein kinase ataxia telangiectasia mutated (ATM) 10 . (nature.com)
• This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. (nih.gov)
• This protein kinase is calcium-independent and phospholipid-dependent. (nih.gov)

Antibodies1

• Abcam: antibodies, proteins, kits. (abcam.com)

Reporter4

• This book chapter will comment on fluorescent reporter proteins and nanocrystals' applicability as fluorescent markers. (intechopen.com)
• This transgenic P. falciparum reporter line constantly expresses two reporter proteins, GFP and NanoLuc, at high levels in two different hosts, human and mosquito. (nagasaki-u.ac.jp)
• By utilising the expression of these two reporter proteins, P. falciparum can be successfully visualised and quantified with high sensitivity. (nagasaki-u.ac.jp)
• We have created a transgenic P. falciparum that expresses two reporter proteins, GFP and NanoLuc, at high levels in both the human- and the vector mosquito stage. (nagasaki-u.ac.jp)

Transgenic1

• Objective 4 is to develop transgenic lines expressing fluorescent and luminescent reporters for high-throughput screening of candidate treatments. (usda.gov)

Markers1

• Highly luminescent QDs could serve as luminescent markers, while magnetic nanoparticles could be easily manipulated under the external magnetic field. (springer.com)

Magnetic1

• Multiplexed immunoassays for proteins using magnetic luminescent nanoparticles for internal calibration. (cdc.gov)

Molecules1

• In this research project, we successfully developed several original methods (or synthetic small molecules) based on chemistry that allow for selective labeling and imaging target proteins under nearly natural conditions. (nii.ac.jp)

Pathologic1

• The fundamental event in prion disease pathogenesis is thought to be the seeded conversion of normal prion protein to its pathologic isoform. (cdc.gov)

Aggregation1

• Design of anti- and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein. (mpg.de)

Cells10

• In future research projects, Wenger and his group want to improve the luminescent properties of the new manganese complexes and anchor them on suitable semiconductor materials for use in solar cells. (phys.org)
• Researchers can introduce these glowing proteins into cells to track all kinds of activity, including the growth of tumors or chatter between nerve cells. (the-scientist.com)
• Heat shock protein (HSP) 90 is abundant in cells, mainly in the form of homodimers, including HSP90α and HSP90β. (spandidos-publications.com)
• As a molecular chaperone, HSP90 regulates the conformational maturation and functional stability of many signaling proteins in cells, serves important roles in cell growth, differentiation, apoptosis and tumor development and is an important antitumor target ( 8 , 9 ). (spandidos-publications.com)
• These methods may contribute to analyzing protein structure and functions in living cells. (nii.ac.jp)
• En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
• Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
• This made the cells luminescent, making them easier to track. (futurity.org)
• [2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
• In conclusion, we showed a multilayered role of DC subsets in priming Tfh cells in protein immunization, and we unveiled the importance of G-CSFR signaling in the development and function pDCs. (bvsalud.org)

Researchers3

• University of Basel researchers have reached an important milestone in their quest to produce more sustainable luminescent materials and catalysts for converting sunlight into other forms of energy. (phys.org)
• Our microplate readers and software are the industry's most cited and have empowered life science researchers to advance protein and cell biology. (moleculardevices.com)
• The researchers created a tightly knit 3D mesh in a hydrogel containing water, hyaluronic acid and short protein sequences associated with brown-fat growth and function. (futurity.org)

Protease1

• Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins. (mpg.de)

Complexes1

• Now, a team led by Professor Oliver Wenger and his doctoral student Patrick Herr from the University of Basel have for the first time succeeded in producing luminescent manganese complexes in which exposure to light causes the same reactions to take place as in ruthenium or iridium compounds. (phys.org)

Aequorin1

• After tediously extracting thousands of specimens to identify the luminescent components back in the 1960s, a scientist named Osamu Shimomura isolated GFP and aequorin, another light-emitting protein, which led to a 2008 Nobel Prize in chemistry. (buzzfeednews.com)

Expression11

• The present study aimed to investigate the effects of changes in heat shock protein (HSP)90β expression and verify whether HSP90β regulates EAAT2 expression in a cerebral ischemia‑reperfusion injury model. (spandidos-publications.com)
• It takes advantage of using a single expression plasmid that contains multiple functionally independent protein expressing cassettes to achieve protein co-expression. (jove.com)
• The main advantage of this technique is the high efficiency of co-expressing cellular fusion proteins in one expression vector. (jove.com)
• Amplify DNA fragments that are necessary for the construction of the semi-independent protein expression cassettes by standard PCR reactions with their corresponding primers and high-fidelity polymerase. (jove.com)
• Mix the DNA fragments designed for the same protein expression cassette together in one PCR tube to a final volume of five microliters. (jove.com)
• Amplify the entire semi-independent protein expression cassette by a second round of PCR. (jove.com)
• Next, linearize the final protein expression backbone vector POC 18, and vector CAMBIA 1300, by adding four units of small one into a final 10 microliter reaction volume. (jove.com)
• HSP90 inhibition may also enhance synaptic protein expression via activation of heat shock factor 1 (HSF-1) transcriptional regulation 16 . (nature.com)
• This is accomplished by increasing both the interaction strength and number of interactions among participant proteins, which is not necessarily accompanied by a change in expression levels. (nature.com)
• As a control, the expression levels of enzyme-fused protein and target proteins were detected using each protein-specific antibody (left panel). (elifesciences.org)
• Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)

Cellular2

• At the sub-cellular scale, maps document the spatial organization of proteins, RNA, DNA, and metabolites with nanometer precision and temporal acuity on the order of seconds. (stanford.edu)
• The prion hypothesis posits that the posttranslational conformational conversion of a host's normal cellular prion protein (PrP C ) by the abnormal form of the prion protein (PrP Sc ) is the fundamental event in prion disease pathogenesis and that PrP Sc itself constitutes the infectious agent. (cdc.gov)

Interactions1

• 2014. Fringe proteins modulate Notch-ligand cis and trans interactions to specifysignaling states. (invivogen.com)

Abundant1

• In addition to nucleosomes, chromatin contains non-histone chromatin-associated proteins, of which the high-mobility group proteins are the most abundant. (nature.com)

Heat shock p1

• Pertinent to AD pathophysiology, heat shock protein 90 (HSP90)/co-chaperone complex folds tau or hyperphosphorylated tau, whereas heat shock protein 70-carboxyl-terminus of HSP70 Interacting protein (HSP70-CHIP) complex mediates degradation 13 , 14 . (nature.com)

Translocation3

• Protein translocation is a fundamental process in biology. (uni-luebeck.de)
• Overall, this technology represents a major step forward, providing a powerful new tool for fundamental mechanistic enquiry of protein translocation and for inhibitor (drug) screening, with an intensity and rigor unattainable through classical methods. (uni-luebeck.de)
• Active DNA demethylation also requires GADD45A (growth arrest and DNA damage protein 45 alpha) and TET1 (ten-eleven translocation methylcytosine dioxygenase 1), which sequentially oxidize 5-methylcytosine (5mC) to 5-carboxylcytosine (5caC) 18 , 19 and are cleared through DNA repair mechanisms. (nature.com)

Transcription4

• However, the order of events and the precise function of high-mobility group proteins during transcription initiation remain unclear. (nature.com)
• Here we show that high-mobility group AT-hook 2 protein (HMGA2) induces DNA nicks at the transcription start site, which are required by the histone chaperone FACT complex to incorporate nucleosomes containing the histone variant H2A.X. Further, phosphorylation of H2A.X at S139 (γ-H2AX) is required for repair-mediated DNA demethylation and transcription activation. (nature.com)
• Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
• Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

Structure2

• 1-- including one high-quality, chromosome-level reference genome assembly using state-of-the-art long-read sequencing technology-- to quantify geographic differences, infer patterns and timing of parasite spread, and identify gene-specific selection, gene function, and structure of essential proteins. (usda.gov)
• Marie Skepö (PI), has in collaboration with Lise Arleth and Kresten Lindorff-Larsen at University of Copenhagen, and Jens Preben Morth at University of Oslo, been awarded 6.8 MNOK for studying the structure of membrane proteins. (lu.se)

Explore1

• Current technical limitations curb our ability to explore global changes in protein level connectivity, restricting how to translate key network changes into AD therapies. (nature.com)

Detection2

• Luminescent conjugated oilgothiphenes are developed for early-stage detection of Alzheimer's disease. (e-science.se)
• Detection of the virus is usually done either by looking for the virus's inner RNA, or pieces of protein on the outside of the virus. (wikipedia.org)

Degradation2

• The ubiquitin-proteasome system (UPS) plays a critical role in protein degradation. (duke.edu)
• Conversely, the degradation of endoplasmic reticulum and misfolded proteins, and of those linked to UPS-independent degradation, is not affected by CLD. (duke.edu)

Inhibits1

• Blasticidin is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes that inhibits protein synthesis by interfering with the peptide-bond formation in the ribosomal machinery. (invivogen.com)

Sensors1

• A bacterium like E. coli is "essentially a very simple cell," with built-in protein sensors to perform its natural functions, Enzymit CEO Gideon Lapidoth said in an interview. (nationaldefensemagazine.org)

Perturbations2

• A protein molecule is an intricate system whose function is highly sensitive to small external perturbations. (rcsb.org)
• However, no examples that correlate protein function with progressive subangstrom structural perturbations have thus far been presented. (rcsb.org)

Regulates1

• Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)

Mediates1

• Here we will focus on HMG AT-hook 2 protein (HMGA2), a member of the HMGA family that mediates transforming growth factor beta 1 (TGFB1, commonly known as TGFβ1) signaling 16 . (nature.com)

Cell biology1

• This method can help answer key questions in the plant molecular and cell biology field such as, how can we proteins in the cell. (jove.com)

Function2

• This protein is therefore highly sensitive to subangstrom deformations and its function must be understood at the subangstrom level. (rcsb.org)
• These results have significant implications for protein function prediction and biomolecule design and engineering, because they suggest methods to tune protein function by modification of the protein scaffold. (rcsb.org)

Light2

• Made possible by the same luminescent proteins that light up jellyfish, a synthesized version is applied to the ice cream to give it its glow. (foodiggity.com)
• Proteins which are involved in the phenomenon of light emission in living systems. (bvsalud.org)

Tissues1

• 0.05) expressed in the cerebellar cortex, piriform lobe, medulla, and corpus callosum of the adult yak while in the young yak brain tissues, the protein expressions were significantly found in the white matter of the cerebellum, pineal gland, corpus callosum, and cerebellar cortex. (scielo.br)

Backbone1

• These structures provide the backbone upon which proteome-wide connectivity, and in turn, protein networks become disturbed and ultimately dysfunctional. (nature.com)

Occurs1

• It is conclusively shown that the ligand-protein binding occurs at the hydrophobic pocket defined by residues Ile360, Thr361, and His362. (e-science.se)

Properties1

• Knowledge of the properties of proteins right down to atomic level is essential for making it easier and faster for the pharmaceutical industry to manufacture protein-based drugs in liquid form. (lu.se)

Human2

• None of the tested prion isolates from diseased animals were as efficient as classical BSE in converting human prion protein. (cdc.gov)
• However, in the case of chronic wasting disease, there was no absolute barrier to conversion of the human prion protein. (cdc.gov)

Target1

• Specifically, the PDCD4 protein combines directly with the mRNA coding region of the target gene ( MYB/c-MYB ) to block translation [2] . (plos.org)

Specifically1

• The cyclophilin-like domain (CLD) of Ran-binding protein-2 (RanBP2/Nup358) associates specifically with at least one subunit, S1, of the base subcomplex of the 19S RP, but the functional implications of this interaction on the UPS activity are elusive. (duke.edu)

Term1

• We introduce the term protein connectivity-based dysfunction (PCBD) to define this mechanism. (nature.com)

Methods1

• We have developed a novel method for co-expressing multiple chimeric fluorescent fusion proteins in plants to overcome the difficulties of conventional methods. (jove.com)