Intestinal Secretions
Jejunum
Intestinal Mucosa
Intestines
Cholera Toxin
Ileum
Immunoglobulin A, Secretory
Enterotoxins
Intestine, Small
Electrolytes
Immunoglobulin A
Cholera
Water-Electrolyte Balance
Bacterial Toxins
Sodium
Theophylline
Diarrhea
Colon
Biological Transport
P-Glycoprotein
Cyclic AMP
Rabbits
Rats, Wistar
Serotonin
Immunoglobulin G
Bodily Secretions
Secretory Rate
Edwardsiella tarda
Edwardsiella ictaluri
Seasons
Fish Diseases
Japan
Neurotensin is a proinflammatory neuropeptide in colonic inflammation. (1/283)
The neuropeptide neurotensin mediates several intestinal functions, including chloride secretion, motility, and cellular growth. However, whether this peptide participates in intestinal inflammation is not known. Toxin A, an enterotoxin from Clostridium difficile, mediates pseudomembranous colitis in humans. In animal models, toxin A causes an acute inflammatory response characterized by activation of sensory neurons and intestinal nerves and immune cells of the lamina propria. Here we show that neurotensin and its receptor are elevated in the rat colonic mucosa following toxin A administration. Pretreatment of rats with the neurotensin receptor antagonist SR-48, 692 inhibits toxin A-induced changes in colonic secretion, mucosal permeability, and histologic damage. Exposure of colonic explants to toxin A or neurotensin causes mast cell degranulation, which is inhibited by SR-48,692. Because substance P was previously shown to mediate mast cell activation, we examined whether substance P is involved in neurotensin-induced mast cell degranulation. Our results show that neurotensin-induced mast cell degranulation in colonic explants is inhibited by the substance P (neurokinin-1) receptor antagonist CP-96,345, indicating that colonic mast activation in response to neurotensin involves release of substance P. We conclude that neurotensin plays a key role in the pathogenesis of C. difficile-induced colonic inflammation and mast cell activation. (+info)Vagotomy inhibits the jejunal fluid secretion activated by luminal ileal Escherichia coli STa in the rat in vivo. (2/283)
BACKGROUND: Escherichia coli heat stable enterotoxin (STa) is a major cause of secretory diarrhoea in humans. AIMS: To assess the effects of instilling STa into the ileum on remote fluid secretion in the jejunum and colon in rats in vivo by a gravimetric technique. METHODS AND RESULTS: Ileal STa (55 ng/ml) stimulated fluid secretion in both ileal and jejunal loops but not in the colon. The fluid secretion induced by ileal STa was inhibited by L-NAME (Nomega-nitro-L-arginine methyl ester, 40 mg/kg intraperitoneally) but not by D-NAME (Nomega-nitro-D-arginine methyl ester). Ileal carbachol (183 mg/ml) instilled into the lumen stimulated ileal secretion but not jejunal secretion, and was unaffected by L-NAME. Capsaicin (10 microM), instilled luminally with STa in the ileum, blocked both the ileal and jejunal fluid secretion. Acute bilateral vagotomy prevented luminal ileal STa from inducing jejunal fluid secretion but not from activating ileal fluid secretion. CONCLUSION: Ileal E coli STa stimulates remote secretion in the rat jejunum but not in the colon, probably by a nitrinergic, vagal reflex mediated by C fibres. This neural pathway will amplify the action of the toxin in its generation of secretory diarrhoea. (+info)The nature of the local immune system of the bovine small intestine. (3/283)
In an examination of the immunoglobulins of the small intestine of calve, IgG1 was the major immunoglobulin in the secretions, and IgA was present in smaller amounts. Immunohistochemical studies showed few IgA containing plasma cells but many cells that stained with anti-IgG antiserum. Histological evidence of transport of IgG1 across the crypt epithelial cell was found. Studies with 125I-labelled IgG1 indicated that a significant proportion of IgG1 in the intestine originated in serum. (+info)Troglitazone inhibits bicarbonate secretion in rat and human duodenum. (4/283)
Troglitazone is a new, orally effective antidiabetic agent that decreases plasma glucose in obese patients with non-insulin-dependent diabetes mellitus. Unfortunately, troglitazone also has a propensity to cause edema. This study was designed to determine how troglitazone affects intestinal ion transport and water absorption. Short circuit current (I(sc)) was measured in rat and human duodenal mucosa in Ussing chambers. Five minutes later, the serosal addition of troglitazone caused I(sc) to decrease gradually, and after 50 min, I(sc) reached the peak of decrease. EC(50) values and maximum response to I(sc) in rat and human mucosa were 8.4 and 8.7 microM and 8.56 +/- 1.0 and 8.00 +/- 2.0 microA/cm(2), respectively. In an HCO(3)(-)/CO(2)-free system, the decrease in I(sc) caused by troglitazone was 1.31 +/- 0.83 microA/cm(2). When 10 mM acetazolamide was preadministered, the small decrease in I(sc) evoked by troglitazone (20 microM) was 4.56 +/- 0.22 microA/cm(2), whereas the preadministration of 100 microM amiloride and 100 nM tetrodotoxin did not influence the decrease in I(sc) evoked by troglitazone. The serosal preadministration of 100 nM vasoactive intestinal peptide potently enhanced the decrease in I(sc) evoked by 20 microM troglitazone (21.1 +/- 1.63 microA/cm(2)). The cyclic AMP contents of rat duodenal mucosa incubated with and without troglitazone (20 microM) for 50 min were 3.2 +/- 0.25 and 5. 8 +/- 0.46 pmol/mg protein, respectively (P <.01). These results indicate that the ionic basis for the decrease in I(sc) that is induced by troglitazone may be inhibition of electrogenic bicarbonate secretion. The alteration of intestinal ion transport by troglitazone could cause edema. (+info)Generation of polymeric immunoglobulin receptor-deficient mouse with marked reduction of secretory IgA. (5/283)
We generated mouse lacking exon 2 of polymeric Ig receptor (pIgR) gene by a gene-targeting strategy (pIgR-deficient mouse; pIgR-/- mouse) to define the physiological role of pIgR in the transcytosis of Igs. pIgR-/- mice were born at the expected ratio from a cross between pIgR+/- mice, indicating that disruption of the pIgR gene in mice is not lethal. pIgR and secretory component proteins were not detected in pIgR-/- mice by Western blot analysis. Moreover, immunohistochemical analysis showed that pIgR protein is not expressed in jejunal and colonic epithelial cells of pIgR-/- mice, whereas IgA+ cells are present in the intestinal mucosa of pIgR-/- mice as well as wild-type littermates. Disruption of the pIgR gene caused a remarkable increase in serum IgA concentration and a slight increment of serum IgG and IgE levels, leaving serum IgM level unaltered. In contrast, IgA was much reduced but not negligible in the bile, feces, and intestinal contents of pIgR-/- mice. Additionally, IgA with a molecular mass of 280 kDa preferentially accumulated in the serum of pIgR-/- mice, suggesting that transepithelial transport of dIgA is severely blocked in pIgR-/- mice. These results demonstrate that dIgA is mainly transported by pIgR on the epithelial cells of intestine and hepatocytes, but a small quantity of IgA may be secreted via other pathways. (+info)Involvement of an organic anion transporter (canalicular multispecific organic anion transporter/multidrug resistance-associated protein 2) in gastrointestinal secretion of glutathione conjugates in rats. (6/283)
We investigated the role of cMOAT/MRP2 (canalicular multispecific organic anion transporter/multidrug resistance-associated protein 2) in the intestinal secretion of organic anions by comparing the behavior in Sprague-Dawley (SD) rats and Eisai hyperbilirubinemic rat (EHBR) whose cMOAT/MRP2 is hereditarily defective. After i.v. administration of 1-chloro-2,4-dinitrobenzene (30 micromol/kg), the biliary and intestinal excretion of its glutathione conjugate 2, 4-dinitrophenyl-S-glutathione (DNP-SG), a substrate for cMOAT/MRP2, was significantly reduced in EHBR compared with SD rats. This result also was confirmed by Ussing chamber studies; DNP-SG showed 1.5-fold greater serosal-to-mucosal flux compared with the mucosal-to-serosal flux in SD rats, whereas a similar flux was observed in both directions in EHBR. In addition, metabolic inhibitors reduced the preferential serosal-to-mucosal flux of DNP-SG in SD rats. In everted sac studies, intestinal secretion clearance, defined as the efflux rate of DNP-SG into the mucosal side divided by the area under the curve on the serosal side, was significantly lower in the jejunum of EHBR than that in SD rats. Northern blot analyses demonstrated the highest mRNA level of cMOAT/MRP2 in the jejunum, which is in good agreement with the results of the everted sac studies. These results suggest that cMOAT/MRP2 is involved in the secretion of organic anions in the small intestine. (+info)Effects of L-glutamine on acetylsalicylic acid-induced gastric lesions in normal and cirrhotic rats. (7/283)
Cirrhosis of the liver in rats was induced by the administration of carbon tetrachloride (0.1 ml/100 g of body weight, s.c.) biweekly for 13 weeks. In the pylorus ligation preparation, acetylsalicylic acid (ASA) 100 mg/kg p.o. induced much more serious gastric damage in CC14-induced cirrhotic rats as compared with rats with a normal liver. L-glutamine 750 mg/kg p.o. prevented the ASA-induced gastric lesions in both normal and cirrhotic rats, even though the degree of the inhibition was weaker in cirrhotic rats. Gastric analysis indicated that L-glutamine 750 mg/kg p.o. markedly inhibited the gastric ionic changes (acid back diffusion) in response to ASA in both cirrhotic and normal rats. (+info)Effect of vasoactive intestinal polypeptide (VIP) antagonism on rat jejunal fluid and electrolyte secretion induced by cholera and Escherichia coli enterotoxins. (8/283)
BACKGROUND: The enteric nervous system is important in the pathophysiology of intestinal fluid secretion induced by cholera toxin (CT), Escherichia coli heat labile (LT), and heat stable (STa) toxins. The neurotransmitters involved are not fully elucidated. Vasoactive intestinal polypeptide (VIP), a potent intestinal secretagogue present in the enteric nervous system, is increased after exposure of the cat intestine to CT. Whether VIP is involved in the pathogenesis of cholera and other toxins in not known. AIM: To study in vivo the effect of VIP antagonism on jejunal fluid secretion induced by CT, LT, and STa. METHODS: CT, LT (25 microg), or 0.9% NaCl was instilled in an isolated 25 cm segment of rat jejunum, and the VIP antagonist (VIPa) [4Cl-D-Phe(6), Leu(17)]-VIP (0.2 or 2 microg/kg/min) or 0.9% NaCl was given intravenously. Two hours later, single pass in vivo jejunal perfusion was performed to assess fluid movement. In STa experiments, intravenous VIPa or 0.9% NaCl was given and 30 minutes later the jejunal segment was perfused with a solution containing STa 200 microg/l. RESULTS: VIPa had no effect on basal intestinal fluid absorption. CT induced net fluid secretion (median -68 microl/min/g dry intestinal weight (interquartile range -80 to -56)) which was dose dependently reversed by VIPa (6.2 (-16 to 34) and 29 (17 to 42); p<0.01). Similarly, LT induced secretion (-63 (-73 to -30)) was attenuated by VIPa (0.2 microg/kg/min) (-15 (-24 to -1); p<0.01) and totally reversed to normal levels by VIPa (2 microg/kg/min) (37 (28-56); p<0. 01 compared with LT and not significant compared with normal controls). STa induced secretion (-17 (-19 to -2)) was also reversed by VIPa (12 (9-23) and 14 (0-26); p<0.01). CONCLUSION: VIP plays an important role in CT, LT, and STa induced intestinal secretion and may be the final putative neurotransmitter in the pathophysiology of these toxins. (+info)Intestinal secretions refer to the fluids and electrolytes that are released by the cells lining the small intestine in response to various stimuli. These secretions play a crucial role in the digestion and absorption of nutrients from food. The major components of intestinal secretions include water, electrolytes (such as sodium, chloride, bicarbonate, and potassium), and enzymes that help break down carbohydrates, proteins, and fats.
The small intestine secretes these substances in response to hormonal signals, neural stimulation, and the presence of food in the lumen of the intestine. The secretion of water and electrolytes helps maintain the proper hydration and pH of the intestinal contents, while the enzymes facilitate the breakdown of nutrients into smaller molecules that can be absorbed across the intestinal wall.
Abnormalities in intestinal secretions can lead to various gastrointestinal disorders, such as diarrhea, malabsorption, and inflammatory bowel disease.
The jejunum is the middle section of the small intestine, located between the duodenum and the ileum. It is responsible for the majority of nutrient absorption that occurs in the small intestine, particularly carbohydrates, proteins, and some fats. The jejunum is characterized by its smooth muscle structure, which allows it to contract and mix food with digestive enzymes and absorb nutrients through its extensive network of finger-like projections called villi.
The jejunum is also lined with microvilli, which further increase the surface area available for absorption. Additionally, the jejunum contains numerous lymphatic vessels called lacteals, which help to absorb fats and fat-soluble vitamins into the bloodstream. Overall, the jejunum plays a critical role in the digestion and absorption of nutrients from food.
The intestinal mucosa is the innermost layer of the intestines, which comes into direct contact with digested food and microbes. It is a specialized epithelial tissue that plays crucial roles in nutrient absorption, barrier function, and immune defense. The intestinal mucosa is composed of several cell types, including absorptive enterocytes, mucus-secreting goblet cells, hormone-producing enteroendocrine cells, and immune cells such as lymphocytes and macrophages.
The surface of the intestinal mucosa is covered by a single layer of epithelial cells, which are joined together by tight junctions to form a protective barrier against harmful substances and microorganisms. This barrier also allows for the selective absorption of nutrients into the bloodstream. The intestinal mucosa also contains numerous lymphoid follicles, known as Peyer's patches, which are involved in immune surveillance and defense against pathogens.
In addition to its role in absorption and immunity, the intestinal mucosa is also capable of producing hormones that regulate digestion and metabolism. Dysfunction of the intestinal mucosa can lead to various gastrointestinal disorders, such as inflammatory bowel disease, celiac disease, and food allergies.
The intestines, also known as the bowel, are a part of the digestive system that extends from the stomach to the anus. They are responsible for the further breakdown and absorption of nutrients from food, as well as the elimination of waste products. The intestines can be divided into two main sections: the small intestine and the large intestine.
The small intestine is a long, coiled tube that measures about 20 feet in length and is lined with tiny finger-like projections called villi, which increase its surface area and enhance nutrient absorption. The small intestine is where most of the digestion and absorption of nutrients takes place.
The large intestine, also known as the colon, is a wider tube that measures about 5 feet in length and is responsible for absorbing water and electrolytes from digested food, forming stool, and eliminating waste products from the body. The large intestine includes several regions, including the cecum, colon, rectum, and anus.
Together, the intestines play a critical role in maintaining overall health and well-being by ensuring that the body receives the nutrients it needs to function properly.
Cholera toxin is a protein toxin produced by the bacterium Vibrio cholerae, which causes the infectious disease cholera. The toxin is composed of two subunits, A and B, and its primary mechanism of action is to alter the normal function of cells in the small intestine.
The B subunit of the toxin binds to ganglioside receptors on the surface of intestinal epithelial cells, allowing the A subunit to enter the cell. Once inside, the A subunit activates a signaling pathway that results in the excessive secretion of chloride ions and water into the intestinal lumen, leading to profuse, watery diarrhea, dehydration, and other symptoms associated with cholera.
Cholera toxin is also used as a research tool in molecular biology and immunology due to its ability to modulate cell signaling pathways. It has been used to study the mechanisms of signal transduction, protein trafficking, and immune responses.
The ileum is the third and final segment of the small intestine, located between the jejunum and the cecum (the beginning of the large intestine). It plays a crucial role in nutrient absorption, particularly for vitamin B12 and bile salts. The ileum is characterized by its thin, lined walls and the presence of Peyer's patches, which are part of the immune system and help surveil for pathogens.
Immunoglobulin A (IgA), Secretory is a type of antibody that plays a crucial role in the immune function of mucous membranes. These membranes line various body openings, such as the respiratory and gastrointestinal tracts, and serve to protect the body from potential pathogens by producing mucus.
Secretory IgA (SIgA) is the primary immunoglobulin found in secretions of the mucous membranes, and it is produced by a special type of immune cell called plasma cells located in the lamina propria, a layer of tissue beneath the epithelial cells that line the mucosal surfaces.
SIgA exists as a dimer, consisting of two IgA molecules linked together by a protein called the J chain. This complex is then transported across the epithelial cell layer to the luminal surface, where it becomes associated with another protein called the secretory component (SC). The SC protects the SIgA from degradation by enzymes and helps it maintain its function in the harsh environment of the mucosal surfaces.
SIgA functions by preventing the attachment and entry of pathogens into the body, thereby neutralizing their infectivity. It can also agglutinate (clump together) microorganisms, making them more susceptible to removal by mucociliary clearance or peristalsis. Furthermore, SIgA can modulate immune responses and contribute to the development of oral tolerance, which is important for maintaining immune homeostasis in the gut.
Enterotoxins are types of toxic substances that are produced by certain microorganisms, such as bacteria. These toxins are specifically designed to target and affect the cells in the intestines, leading to symptoms such as diarrhea, vomiting, and abdominal cramps. One well-known example of an enterotoxin is the toxin produced by Staphylococcus aureus bacteria, which can cause food poisoning. Another example is the cholera toxin produced by Vibrio cholerae, which can cause severe diarrhea and dehydration. Enterotoxins work by interfering with the normal functioning of intestinal cells, leading to fluid accumulation in the intestines and subsequent symptoms.
Intestinal absorption refers to the process by which the small intestine absorbs water, nutrients, and electrolytes from food into the bloodstream. This is a critical part of the digestive process, allowing the body to utilize the nutrients it needs and eliminate waste products. The inner wall of the small intestine contains tiny finger-like projections called villi, which increase the surface area for absorption. Nutrients are absorbed into the bloodstream through the walls of the capillaries in these villi, and then transported to other parts of the body for use or storage.
Precipitins are antibodies (usually of the IgG class) that, when combined with their respective antigens in vitro, result in the formation of a visible precipitate. They are typically produced in response to the presence of insoluble antigens, such as bacterial or fungal cell wall components, and can be detected through various immunological techniques such as precipitation tests (e.g., Ouchterlony double diffusion, radial immunodiffusion).
Precipitins are often used in the diagnosis of infectious diseases, autoimmune disorders, and allergies to identify the presence and specificity of antibodies produced against certain antigens. However, it's worth noting that the term "precipitin" is not commonly used in modern medical literature, and the more general term "antibody" is often preferred.
The small intestine is the portion of the gastrointestinal tract that extends from the pylorus of the stomach to the beginning of the large intestine (cecum). It plays a crucial role in the digestion and absorption of nutrients from food. The small intestine is divided into three parts: the duodenum, jejunum, and ileum.
1. Duodenum: This is the shortest and widest part of the small intestine, approximately 10 inches long. It receives chyme (partially digested food) from the stomach and begins the process of further digestion with the help of various enzymes and bile from the liver and pancreas.
2. Jejunum: The jejunum is the middle section, which measures about 8 feet in length. It has a large surface area due to the presence of circular folds (plicae circulares), finger-like projections called villi, and microvilli on the surface of the absorptive cells (enterocytes). These structures increase the intestinal surface area for efficient absorption of nutrients, electrolytes, and water.
3. Ileum: The ileum is the longest and final section of the small intestine, spanning about 12 feet. It continues the absorption process, mainly of vitamin B12, bile salts, and any remaining nutrients. At the end of the ileum, there is a valve called the ileocecal valve that prevents backflow of contents from the large intestine into the small intestine.
The primary function of the small intestine is to absorb the majority of nutrients, electrolytes, and water from ingested food. The mucosal lining of the small intestine contains numerous goblet cells that secrete mucus, which protects the epithelial surface and facilitates the movement of chyme through peristalsis. Additionally, the small intestine hosts a diverse community of microbiota, which contributes to various physiological functions, including digestion, immunity, and protection against pathogens.
Chlorides are simple inorganic ions consisting of a single chlorine atom bonded to a single charged hydrogen ion (H+). Chloride is the most abundant anion (negatively charged ion) in the extracellular fluid in the human body. The normal range for chloride concentration in the blood is typically between 96-106 milliequivalents per liter (mEq/L).
Chlorides play a crucial role in maintaining electrical neutrality, acid-base balance, and osmotic pressure in the body. They are also essential for various physiological processes such as nerve impulse transmission, maintenance of membrane potentials, and digestion (as hydrochloric acid in the stomach).
Chloride levels can be affected by several factors, including diet, hydration status, kidney function, and certain medical conditions. Increased or decreased chloride levels can indicate various disorders, such as dehydration, kidney disease, Addison's disease, or diabetes insipidus. Therefore, monitoring chloride levels is essential for assessing a person's overall health and diagnosing potential medical issues.
Electrolytes are substances that, when dissolved in water, break down into ions that can conduct electricity. In the body, electrolytes are responsible for regulating various important physiological functions, including nerve and muscle function, maintaining proper hydration and acid-base balance, and helping to repair tissue damage.
The major electrolytes found in the human body include sodium, potassium, chloride, bicarbonate, calcium, magnesium, and phosphate. These electrolytes are tightly regulated by various mechanisms, including the kidneys, which help to maintain their proper balance in the body.
When there is an imbalance of electrolytes in the body, it can lead to a range of symptoms and health problems. For example, low levels of sodium (hyponatremia) can cause confusion, seizures, and even coma, while high levels of potassium (hyperkalemia) can lead to heart arrhythmias and muscle weakness.
Electrolytes are also lost through sweat during exercise or illness, so it's important to replace them through a healthy diet or by drinking fluids that contain electrolytes, such as sports drinks or coconut water. In some cases, electrolyte imbalances may require medical treatment, such as intravenous (IV) fluids or medication.
Immunoglobulin A (IgA) is a type of antibody that plays a crucial role in the immune function of the human body. It is primarily found in external secretions, such as saliva, tears, breast milk, and sweat, as well as in mucous membranes lining the respiratory and gastrointestinal tracts. IgA exists in two forms: a monomeric form found in serum and a polymeric form found in secretions.
The primary function of IgA is to provide immune protection at mucosal surfaces, which are exposed to various environmental antigens, such as bacteria, viruses, parasites, and allergens. By doing so, it helps prevent the entry and colonization of pathogens into the body, reducing the risk of infections and inflammation.
IgA functions by binding to antigens present on the surface of pathogens or allergens, forming immune complexes that can neutralize their activity. These complexes are then transported across the epithelial cells lining mucosal surfaces and released into the lumen, where they prevent the adherence and invasion of pathogens.
In summary, Immunoglobulin A (IgA) is a vital antibody that provides immune defense at mucosal surfaces by neutralizing and preventing the entry of harmful antigens into the body.
Cholera is an infectious disease caused by the bacterium Vibrio cholerae, which is usually transmitted through contaminated food or water. The main symptoms of cholera are profuse watery diarrhea, vomiting, and dehydration, which can lead to electrolyte imbalances, shock, and even death if left untreated. Cholera remains a significant public health concern in many parts of the world, particularly in areas with poor sanitation and hygiene. The disease is preventable through proper food handling, safe water supplies, and improved sanitation, as well as vaccination for those at high risk.
Water-electrolyte balance refers to the regulation of water and electrolytes (sodium, potassium, chloride, bicarbonate) in the body to maintain homeostasis. This is crucial for various bodily functions such as nerve impulse transmission, muscle contraction, fluid balance, and pH regulation. The body maintains this balance through mechanisms that control water intake, excretion, and electrolyte concentration in various body fluids like blood and extracellular fluid. Disruptions in water-electrolyte balance can lead to dehydration or overhydration, and imbalances in electrolytes can cause conditions such as hyponatremia (low sodium levels) or hyperkalemia (high potassium levels).
Bacterial toxins are poisonous substances produced and released by bacteria. They can cause damage to the host organism's cells and tissues, leading to illness or disease. Bacterial toxins can be classified into two main types: exotoxins and endotoxins.
Exotoxins are proteins secreted by bacterial cells that can cause harm to the host. They often target specific cellular components or pathways, leading to tissue damage and inflammation. Some examples of exotoxins include botulinum toxin produced by Clostridium botulinum, which causes botulism; diphtheria toxin produced by Corynebacterium diphtheriae, which causes diphtheria; and tetanus toxin produced by Clostridium tetani, which causes tetanus.
Endotoxins, on the other hand, are components of the bacterial cell wall that are released when the bacteria die or divide. They consist of lipopolysaccharides (LPS) and can cause a generalized inflammatory response in the host. Endotoxins can be found in gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa.
Bacterial toxins can cause a wide range of symptoms depending on the type of toxin, the dose, and the site of infection. They can lead to serious illnesses or even death if left untreated. Vaccines and antibiotics are often used to prevent or treat bacterial infections and reduce the risk of severe complications from bacterial toxins.
Sodium is an essential mineral and electrolyte that is necessary for human health. In a medical context, sodium is often discussed in terms of its concentration in the blood, as measured by serum sodium levels. The normal range for serum sodium is typically between 135 and 145 milliequivalents per liter (mEq/L).
Sodium plays a number of important roles in the body, including:
* Regulating fluid balance: Sodium helps to regulate the amount of water in and around your cells, which is important for maintaining normal blood pressure and preventing dehydration.
* Facilitating nerve impulse transmission: Sodium is involved in the generation and transmission of electrical signals in the nervous system, which is necessary for proper muscle function and coordination.
* Assisting with muscle contraction: Sodium helps to regulate muscle contractions by interacting with other minerals such as calcium and potassium.
Low sodium levels (hyponatremia) can cause symptoms such as confusion, seizures, and coma, while high sodium levels (hypernatremia) can lead to symptoms such as weakness, muscle cramps, and seizures. Both conditions require medical treatment to correct.
Theophylline is a medication that belongs to a class of drugs called methylxanthines. It is used in the management of respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and other conditions that cause narrowing of the airways in the lungs.
Theophylline works by relaxing the smooth muscle around the airways, which helps to open them up and make breathing easier. It also acts as a bronchodilator, increasing the flow of air into and out of the lungs. Additionally, theophylline has anti-inflammatory effects that can help reduce swelling in the airways and relieve symptoms such as coughing, wheezing, and shortness of breath.
Theophylline is available in various forms, including tablets, capsules, and liquid solutions. It is important to take this medication exactly as prescribed by a healthcare provider, as the dosage may vary depending on individual factors such as age, weight, and liver function. Regular monitoring of blood levels of theophylline is also necessary to ensure safe and effective use of the medication.
Diarrhea is a condition in which an individual experiences loose, watery stools frequently, often exceeding three times a day. It can be acute, lasting for several days, or chronic, persisting for weeks or even months. Diarrhea can result from various factors, including viral, bacterial, or parasitic infections, food intolerances, medications, and underlying medical conditions such as inflammatory bowel disease or irritable bowel syndrome. Dehydration is a potential complication of diarrhea, particularly in severe cases or in vulnerable populations like young children and the elderly.
The colon, also known as the large intestine, is a part of the digestive system in humans and other vertebrates. It is an organ that eliminates waste from the body and is located between the small intestine and the rectum. The main function of the colon is to absorb water and electrolytes from digested food, forming and storing feces until they are eliminated through the anus.
The colon is divided into several regions, including the cecum, ascending colon, transverse colon, descending colon, sigmoid colon, rectum, and anus. The walls of the colon contain a layer of muscle that helps to move waste material through the organ by a process called peristalsis.
The inner surface of the colon is lined with mucous membrane, which secretes mucus to lubricate the passage of feces. The colon also contains a large population of bacteria, known as the gut microbiota, which play an important role in digestion and immunity.
Biological transport refers to the movement of molecules, ions, or solutes across biological membranes or through cells in living organisms. This process is essential for maintaining homeostasis, regulating cellular functions, and enabling communication between cells. There are two main types of biological transport: passive transport and active transport.
Passive transport does not require the input of energy and includes:
1. Diffusion: The random movement of molecules from an area of high concentration to an area of low concentration until equilibrium is reached.
2. Osmosis: The diffusion of solvent molecules (usually water) across a semi-permeable membrane from an area of lower solute concentration to an area of higher solute concentration.
3. Facilitated diffusion: The assisted passage of polar or charged substances through protein channels or carriers in the cell membrane, which increases the rate of diffusion without consuming energy.
Active transport requires the input of energy (in the form of ATP) and includes:
1. Primary active transport: The direct use of ATP to move molecules against their concentration gradient, often driven by specific transport proteins called pumps.
2. Secondary active transport: The coupling of the movement of one substance down its electrochemical gradient with the uphill transport of another substance, mediated by a shared transport protein. This process is also known as co-transport or counter-transport.
P-glycoprotein (P-gp) is a type of membrane transport protein that plays a crucial role in the efflux (extrusion) of various substrates, including drugs and toxins, out of cells. It is also known as multidrug resistance protein 1 (MDR1).
P-gp is encoded by the ABCB1 gene and is primarily located on the apical membrane of epithelial cells in several tissues, such as the intestine, liver, kidney, and blood-brain barrier. Its main function is to protect these organs from harmful substances by actively pumping them out of the cells and back into the lumen or bloodstream.
In the context of pharmacology, P-gp can contribute to multidrug resistance (MDR) in cancer cells. When overexpressed, P-gp can reduce the intracellular concentration of various anticancer drugs, making them less effective. This has led to extensive research on inhibitors of P-gp as potential adjuvants for cancer therapy.
In summary, P-glycoprotein is a vital efflux transporter that helps maintain homeostasis by removing potentially harmful substances from cells and can impact drug disposition and response in various tissues, including the intestine, liver, kidney, and blood-brain barrier.
Cyclic adenosine monophosphate (cAMP) is a key secondary messenger in many biological processes, including the regulation of metabolism, gene expression, and cellular excitability. It is synthesized from adenosine triphosphate (ATP) by the enzyme adenylyl cyclase and is degraded by the enzyme phosphodiesterase.
In the body, cAMP plays a crucial role in mediating the effects of hormones and neurotransmitters on target cells. For example, when a hormone binds to its receptor on the surface of a cell, it can activate a G protein, which in turn activates adenylyl cyclase to produce cAMP. The increased levels of cAMP then activate various effector proteins, such as protein kinases, which go on to regulate various cellular processes.
Overall, the regulation of cAMP levels is critical for maintaining proper cellular function and homeostasis, and abnormalities in cAMP signaling have been implicated in a variety of diseases, including cancer, diabetes, and neurological disorders.
I believe there may be some confusion in your question. "Rabbits" is a common name used to refer to the Lagomorpha species, particularly members of the family Leporidae. They are small mammals known for their long ears, strong legs, and quick reproduction.
However, if you're referring to "rabbits" in a medical context, there is a term called "rabbit syndrome," which is a rare movement disorder characterized by repetitive, involuntary movements of the fingers, resembling those of a rabbit chewing. It is also known as "finger-chewing chorea." This condition is usually associated with certain medications, particularly antipsychotics, and typically resolves when the medication is stopped or adjusted.
"Wistar rats" are a strain of albino rats that are widely used in laboratory research. They were developed at the Wistar Institute in Philadelphia, USA, and were first introduced in 1906. Wistar rats are outbred, which means that they are genetically diverse and do not have a fixed set of genetic characteristics like inbred strains.
Wistar rats are commonly used as animal models in biomedical research because of their size, ease of handling, and relatively low cost. They are used in a wide range of research areas, including toxicology, pharmacology, nutrition, cancer, cardiovascular disease, and behavioral studies. Wistar rats are also used in safety testing of drugs, medical devices, and other products.
Wistar rats are typically larger than many other rat strains, with males weighing between 500-700 grams and females weighing between 250-350 grams. They have a lifespan of approximately 2-3 years. Wistar rats are also known for their docile and friendly nature, making them easy to handle and work with in the laboratory setting.
Serotonin, also known as 5-hydroxytryptamine (5-HT), is a monoamine neurotransmitter that is found primarily in the gastrointestinal (GI) tract, blood platelets, and the central nervous system (CNS) of humans and other animals. It is produced by the conversion of the amino acid tryptophan to 5-hydroxytryptophan (5-HTP), and then to serotonin.
In the CNS, serotonin plays a role in regulating mood, appetite, sleep, memory, learning, and behavior, among other functions. It also acts as a vasoconstrictor, helping to regulate blood flow and blood pressure. In the GI tract, it is involved in peristalsis, the contraction and relaxation of muscles that moves food through the digestive system.
Serotonin is synthesized and stored in serotonergic neurons, which are nerve cells that use serotonin as their primary neurotransmitter. These neurons are found throughout the brain and spinal cord, and they communicate with other neurons by releasing serotonin into the synapse, the small gap between two neurons.
Abnormal levels of serotonin have been linked to a variety of disorders, including depression, anxiety, schizophrenia, and migraines. Medications that affect serotonin levels, such as selective serotonin reuptake inhibitors (SSRIs), are commonly used to treat these conditions.
Immunoglobulin G (IgG) is a type of antibody, which is a protective protein produced by the immune system in response to foreign substances like bacteria or viruses. IgG is the most abundant type of antibody in human blood, making up about 75-80% of all antibodies. It is found in all body fluids and plays a crucial role in fighting infections caused by bacteria, viruses, and toxins.
IgG has several important functions:
1. Neutralization: IgG can bind to the surface of bacteria or viruses, preventing them from attaching to and infecting human cells.
2. Opsonization: IgG coats the surface of pathogens, making them more recognizable and easier for immune cells like neutrophils and macrophages to phagocytose (engulf and destroy) them.
3. Complement activation: IgG can activate the complement system, a group of proteins that work together to help eliminate pathogens from the body. Activation of the complement system leads to the formation of the membrane attack complex, which creates holes in the cell membranes of bacteria, leading to their lysis (destruction).
4. Antibody-dependent cellular cytotoxicity (ADCC): IgG can bind to immune cells like natural killer (NK) cells and trigger them to release substances that cause target cells (such as virus-infected or cancerous cells) to undergo apoptosis (programmed cell death).
5. Immune complex formation: IgG can form immune complexes with antigens, which can then be removed from the body through various mechanisms, such as phagocytosis by immune cells or excretion in urine.
IgG is a critical component of adaptive immunity and provides long-lasting protection against reinfection with many pathogens. It has four subclasses (IgG1, IgG2, IgG3, and IgG4) that differ in their structure, function, and distribution in the body.
Bodily secretions are substances that are produced and released by various glands and organs in the body. These secretions help maintain the body's homeostasis, protect it from external threats, and aid in digestion and other physiological processes. Examples of bodily secretions include:
1. Sweat: A watery substance produced by sweat glands to regulate body temperature through evaporation.
2. Sebaceous secretions: Oily substances produced by sebaceous glands to lubricate and protect the skin and hair.
3. Saliva: A mixture of water, enzymes, electrolytes, and mucus produced by salivary glands to aid in digestion and speech.
4. Tears: A mixture of water, electrolytes, and proteins produced by the lacrimal glands to lubricate and protect the eyes.
5. Mucus: A slippery substance produced by mucous membranes lining various body cavities, such as the respiratory and gastrointestinal tracts, to trap and remove foreign particles and pathogens.
6. Gastric juices: Digestive enzymes and hydrochloric acid produced by the stomach to break down food.
7. Pancreatic juices: Digestive enzymes produced by the pancreas to further break down food in the small intestine.
8. Bile: A greenish-brown alkaline fluid produced by the liver and stored in the gallbladder, which helps digest fats and eliminate waste products.
9. Menstrual blood: The shedding of the uterine lining that occurs during menstruation, containing blood, mucus, and endometrial tissue.
10. Vaginal secretions: Fluid produced by the vagina to maintain its moisture, pH balance, and provide a protective barrier against infections.
11. Semen: A mixture of sperm cells, fluids from the seminal vesicles, prostate gland, and bulbourethral glands that aids in the transportation and survival of sperm during sexual reproduction.
Secretory rate refers to the amount or volume of a secretion produced by a gland or an organ over a given period of time. It is a measure of the productivity or activity level of the secreting structure. The secretory rate can be quantified for various bodily fluids, such as saliva, sweat, digestive enzymes, hormones, or milk, depending on the context and the specific gland or organ being studied.
In clinical settings, measuring the secretory rate might involve collecting and analyzing samples over a certain duration to estimate the production rate of the substance in question. This information can be helpful in diagnosing conditions related to impaired secretion, monitoring treatment responses, or understanding the physiological adaptations of the body under different circumstances.
'Edwardsiella tarda' is a gram-negative, rod-shaped bacterium that can cause various infections in humans, animals, and fish. It is named after Francis E. Edwards, an American microbiologist who first isolated the bacterium in 1965. The bacterium is found in aquatic environments, including freshwater and brackish water, as well as in the intestines of animals and fish.
In humans, 'E. tarda' can cause a range of infections, including gastroenteritis, wound infections, meningitis, and sepsis. The bacterium is often associated with exposure to contaminated water or food, particularly raw or undercooked seafood. People with weakened immune systems, such as those with liver disease or cancer, are at higher risk of developing severe infections.
Treatment for 'E. tarda' infections typically involves antibiotics, such as ciprofloxacin or trimethoprim-sulfamethoxazole. Prevention measures include practicing good hygiene, avoiding consumption of raw or undercooked seafood, and promptly treating any wounds that come into contact with contaminated water.
'Edwardsiella ictaluri' is a gram-negative, rod-shaped bacterium that belongs to the family Enterobacteriaceae. It is a facultative anaerobe, which means it can grow in both the presence and absence of oxygen. This bacterium is known to cause enteric septicemia of catfish (ESC), a significant disease in farm-raised catfish in the United States. The infection can lead to high mortality rates in young fish, causing significant economic losses for the aquaculture industry. It's essential to maintain proper biosecurity measures and use effective vaccines to control ESC in farmed catfish.
I'm sorry for any confusion, but "seasons" is not a term that has a medical definition. Seasons refer to the four divisions of the year (spring, summer, autumn or fall, and winter) based on the position of the earth in its orbit around the sun. If you have any questions related to health or medicine, I'd be happy to try to help answer those!
Enterobacteriaceae are a large family of gram-negative bacteria that are commonly found in the human gut and surrounding environment. Infections caused by Enterobacteriaceae can occur when these bacteria enter parts of the body where they are not normally present, such as the bloodstream, urinary tract, or abdominal cavity.
Enterobacteriaceae infections can cause a range of symptoms depending on the site of infection. For example:
* Urinary tract infections (UTIs) caused by Enterobacteriaceae may cause symptoms such as frequent urination, pain or burning during urination, and lower abdominal pain.
* Bloodstream infections (bacteremia) caused by Enterobacteriaceae can cause fever, chills, and sepsis, a potentially life-threatening condition characterized by a whole-body inflammatory response to infection.
* Pneumonia caused by Enterobacteriaceae may cause cough, chest pain, and difficulty breathing.
* Intra-abdominal infections (such as appendicitis or diverticulitis) caused by Enterobacteriaceae can cause abdominal pain, fever, and changes in bowel habits.
Enterobacteriaceae infections are typically treated with antibiotics, but the increasing prevalence of antibiotic-resistant strains of these bacteria has made treatment more challenging in recent years. Preventing the spread of Enterobacteriaceae in healthcare settings and promoting good hygiene practices can help reduce the risk of infection.
"Fish diseases" is a broad term that refers to various health conditions and infections affecting fish populations in aquaculture, ornamental fish tanks, or wild aquatic environments. These diseases can be caused by bacteria, viruses, fungi, parasites, or environmental factors such as water quality, temperature, and stress.
Some common examples of fish diseases include:
1. Bacterial diseases: Examples include furunculosis (caused by Aeromonas salmonicida), columnaris disease (caused by Flavobacterium columnare), and enteric septicemia of catfish (caused by Edwardsiella ictaluri).
2. Viral diseases: Examples include infectious pancreatic necrosis virus (IPNV) in salmonids, viral hemorrhagic septicemia virus (VHSV), and koi herpesvirus (KHV).
3. Fungal diseases: Examples include saprolegniasis (caused by Saprolegnia spp.) and cotton wool disease (caused by Aphanomyces spp.).
4. Parasitic diseases: Examples include ichthyophthirius multifiliis (Ich), costia, trichodina, and various worm infestations such as anchor worms (Lernaea spp.) and tapeworms (Diphyllobothrium spp.).
5. Environmental diseases: These are caused by poor water quality, temperature stress, or other environmental factors that weaken the fish's immune system and make them more susceptible to infections. Examples include osmoregulatory disorders, ammonia toxicity, and low dissolved oxygen levels.
It is essential to diagnose and treat fish diseases promptly to prevent their spread among fish populations and maintain healthy aquatic ecosystems. Preventative measures such as proper sanitation, water quality management, biosecurity practices, and vaccination can help reduce the risk of fish diseases in both farmed and ornamental fish settings.
I'm sorry for any confusion, but "Japan" is not a medical term. Japan is the name of a country, officially known as Nippon-koku or Nihon-koku in Japanese, and is located in East Asia. It is an island nation in the Pacific Ocean with a population of about 126 million people.
If you have any medical questions or terms that you would like me to define, please let me know!
I'm sorry for any confusion, but "flounder" is not a medical term. It is a common name used for several species of flatfish that are widely consumed as food. If you have any questions about medical terminology or health-related topics, please don't hesitate to ask!
Intestinal mucosal barrier
Timeline of human prenatal development
Intestinal epithelium
Secretin
Vasoactive intestinal peptide
Oleandrin
Helen Cooke
Serotonin
Substance P
Haarlem oil
Blastocystosis
ACTH stimulation test
Yip1 domain family
Malabsorption
Enteric nervous system
Gut-brain axis
GLUT5
Sphincter of Oddi
Trefoil factor 3
Kim Barrett
Lester Dragstedt
Oral rehydration therapy
Alkaline phosphatase
List of intestinal stem cell marker genes
Chlorquinaldol
Chyme
PSMD4
CD36
Alpha defensin
Paneth cell
APOA4
WikiGenes - Intestinal Secretions
Frontiers | Plantain flour: A potential anti-obesity ingredient for intestinal flora regulation and improved hormone secretion
Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the...
The Role of mdr1a P-Glycoprotein in the Biliary and Intestinal Secretion of Doxorubicin and Vinblastine in Mice | Drug...
Effect of vasoactive intestinal polypeptide (VIP) antagonism on rat jejunal fluid and electrolyte secretion induced by cholera...
Vectorial secretion of interleukin-8 mediates autocrine signalling in intestinal epithelial cells via apically located CXCR1 |...
EN (en)
Transfer of intestinal bacterial components to mammary secretions in the cow - Waikato Medical Research Foundation
Intestinal mucosal barrier - Wikipedia
The impact of lactoferrin with different levels of metal saturation on the intestinal epithelial barrier function and mucosal...
Immunonutrition enhances the expression and secretion of mouse intestinal defensins [6]<...
Elevated Serotonin in COVID-19 Stimulates Chloride Secretion in Lung and Intestinal Epithelial Cells - Just Science -...
Melanie Blokesch - People - EPFL
NIH Sensory Nutrition and Disease Workshop - American Society for Nutrition
Hypoglycemic Effects of Intestinal Electrical Stimulation by Enhancing Nutrient-Stimulated Secretion of GLP-1 in Rats<...
Campylobacter Infections: Background, Pathophysiology, Epidemiology
ALOE FEROX plant extract (a laxative agent in South Africa) increased intestinal secretion and motility in constipated rats -...
Bioline International Official Site (site up-dated regularly)
Gastrointestinal Disorders Quiz Questions And Answers - ProProfs Quiz
Food Poisoning Workup: Laboratory Studies, Imaging Studies, Procedures
Drivers and determinants of strain dynamics following fecal microbiota transplantation | Nature Medicine
vasoactive intestinal polypeptide
JCI - Intestinal lipid-derived signals that sense dietary fat
Edwardsiella tarda Bacteremia, Okayama, Japan, 2005-2016 - Volume 25, Number 10-October 2019 - Emerging Infectious Diseases...
CARTPT CART prepropeptide [Homo sapiens (human)] - Gene - NCBI
IJMS | Free Full-Text | Beyond the Flavour: The Potential Druggability of Chemosensory G Protein-Coupled Receptors
Adipose Tissue-Derived Biomarkers of Intestinal Barrier Functions for the Characterization of Diarrhoea-Predominant IBS
DNA methyltransferase 3A controls intestinal epithelial barrier function and regeneration in the colon | Nature Communications
Intestinal Organoid Mini-Review
Lumen15
- Thus, the absence of mdr1a P-glycoprotein affects the fate of vinblastine chiefly by diminishing secretion into the lumen of the small intestine, whereas it affects the fate of doxorubicin chiefly by diminishing secretion of parent drug into bile. (aspetjournals.org)
- These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
- The central component is the intestinal epithelial layer, which provides physical separation between the lumen and the body. (wikipedia.org)
- The secretion of various molecules into the lumen reinforces the barrier function on the extra-epithelial side, while a variety of immune cells provide additional protection below the epithelial layer. (wikipedia.org)
- In addition to its protective function, the intestinal epithelium controls the selective uptake of beneficial ions, nutrients, and other substances from the lumen into the body. (wikipedia.org)
- Specialised secretory epithelial cells called Paneth cells secrete abundant quantities human α-defensins into the intestinal lumen of healthy individuals. (wikipedia.org)
- Secretory immunoglobulin A (sIgA) is produced by plasma cells in the lamina propria and transported into the lumen by intestinal epithelial cells. (wikipedia.org)
- Translocation of bacteria, primarily Gram-negative pathogenic flora, from the intestinal lumen into the circulatory system leads to sepsis. (springer.com)
- The infections may originate from a hospital environment or may arise from the translocation of microorganisms from the intestinal lumen to the peripheral blood supply. (springer.com)
- Intestinal dysbiosis, as well as immaturity of the gut and mucosal immunity, then contribute to a loss of epithelium integrity and the translocation of microorganisms from the lumen to the peripheral blood supply. (springer.com)
- Intestinal epithelial cells (IECs) have evolved to build a complex physiological barrier that separates the intestinal lumen from the underlying mucosal tissue. (nature.com)
- These organoids include a polarized epithelial layer that surrounds a functional lumen and contains cell types of the intestinal epithelium present in proportions and relative spatial arrangement that mimic what is observed in vivo. (stemcell.com)
- Absorbed nitrate may re-enter the stomach and intestinal lumen directly via the bloodstream and via secretions. (inchem.org)
- Intestinal organoids are 3D microtissue models that recapitulate structures in the intestinal lumen and on the surrounding intestinal epithelium. (moleculardevices.com)
- Expulsion of meconium from the intestinal lumen into the amniotic cavity is a consequence of increased intestinal peristalsis and of anal sphincter relaxation resulting from vagal stimulation [ 3 ]. (hindawi.com)
Epithelium19
- In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. (biomedcentral.com)
- Mucus forms a layer (or layers, in the case of the colon) that separates the bulk of the luminal contents from the intestinal epithelium. (wikipedia.org)
- The intestinal epithelium is the foremost component of the intestinal mucosal barrier. (wikipedia.org)
- A variety of immune cells reside in the lamina propria which underlies the intestinal epithelium. (wikipedia.org)
- This population provides immune protection that is characterised by the rapid detection and killing of microorganisms that penetrate the intestinal epithelium. (wikipedia.org)
- Intestinal permeability is a measurement of intestinal mucosal barrier function and is defined as "the facility with which intestinal epithelium allows molecules to pass through by non-mediated passive diffusion. (wikipedia.org)
- parasitizes intestinal epithelium, resulting in enterocyte loss, villous atrophy, and malabsorptive diarrhea. (ncsu.edu)
- Cryptosporidium parvum is a minimally invasive protozoal pathogen of intestinal epithelium that results in villus atrophy, mucosal lipid peroxidation, diarrhea, and diminished barrier function. (ncsu.edu)
- Cryptosporidium parvum parasitizes intestinal epithelium, resulting in loss of epithelial cells and mucosal inflammation. (ncsu.edu)
- Using western blot, immunostaining, and electrophysiology, we characterized the localization of human ANO1 and its stimulation by 5-HT on Cl secretion in lung and intestinal epithelium. (cancertreatmentsresearch.com)
- Finally, we demonstrate that SARS-CoV2 infection led to enterochromaffin cell hyperplasia in the intestinal epithelium of Syrian Hamster with a possible elevation of 5-HT, which could explain the severity of symptoms in COVID-19 associated diarrheal patients. (cancertreatmentsresearch.com)
- Elevated 5-HT activates luminal ANO1 CaCC in the intestinal and lung epithelium by a mechanism that appears to involve the rise of [Ca 2+ ] i . (cancertreatmentsresearch.com)
- Several functional components of the epithelium contribute to this unique role, among them the secretion of a mucus layer and life-long regeneration of the different epithelial cell types from a stem cell compartment, allowing for quick repair of barrier defects 2 . (nature.com)
- In the last decade there has been a dramatic shift in the availability of tools and model systems used to study the intestinal epithelium. (stemcell.com)
- The intestinal epithelium incorporates several distinct cell populations, including the rapidly dividing intestinal stem cells (ISCs) that facilitate the typical four-to-five day turnover cycle of the adult intestinal epithelium. (stemcell.com)
- Diagram of the small intestinal epithelium highlighting the identity and spatial arrangement of key epithelial cell types. (stemcell.com)
- The adult intestinal epithelium is primarily composed of six cell types that are arranged in a crypt-villus structure (Figure 1). (stemcell.com)
- The epithelium contains the essential cell populations found in the adult intestinal epithelium and is arranged in a crypt-villus structure. (moleculardevices.com)
- The cell composition and arrangement of the epithelium make intestinal organoids useful for studying intestinal cell biology, regeneration, differentiation, as wells as diseases phenotypes including effects of specific mutations, microbiome, or inflammation process. (moleculardevices.com)
Mucosa6
- In the intestinal mucosa, several adaptations of TLR signalling have evolved to avoid chronic inflammatory responses to the presence of commensal microbes. (biomedcentral.com)
- The intestinal mucosal barrier, also referred to as intestinal barrier, refers to the property of the intestinal mucosa that ensures adequate containment of undesirable luminal contents within the intestine while preserving the ability to absorb nutrients. (wikipedia.org)
- The intestinal mucosal barrier is a heterogeneous entity composed of physical, biochemical, and immune elements elaborated by the intestinal mucosa. (wikipedia.org)
- Both contribute to intestinal barrier function although they are not produced by the intestinal mucosa. (wikipedia.org)
- Using an entire intestinal mucosa, we have demonstrated for the first time that NO serves as a proximal mediator of PGE2 synthesis and barrier function in C. parvum infection. (ncsu.edu)
- It stimulates the secretion of electrolytes and water by the intestinal mucosa. (britannica.com)
Permeability6
- Intestinal barrier integrity is malleable and multiple mechanisms have been shown to be capable of modulating intestinal permeability (a measure of intestinal barrier function). (wikipedia.org)
- Cryptosporidiosis induced severe watery diarrhea lasting >9 d and produced a 25% increase in intestinal permeability, a 33% decrease in villous surface area, and a 40% reduction in mucosal lactase specific activity. (ncsu.edu)
- Alterations of the small-intestinal permeability (s-IP) might play an essential role in a subgroup of diarrhoea-predominant IBS (D-IBS) patients. (hindawi.com)
- A dysfunctional gut barrier leads to variations of small intestinal permeability (s-IP) and could be the origin or the consequence of the persistent, low-grade immune activation characterising the abovementioned D-IBS subtype and, with greater severity, other inflammatory GI diseases, such as inflammatory bowel disease (IBD) and celiac disease (CD) [ 6 , 9 ]. (hindawi.com)
- Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
- C-BF reduced LPS induced intestinal histological damage and gut permeability to 4 KD Fluorescein-isothiocyanate-conjugated dextran. (unboundmedicine.com)
Chloride Secretion1
- To get a little technical, it causes increased chloride secretion by intestinal crypt cells and decreased sodium chloride coupled absorption by villus cells. (cdc.gov)
Expression and secretion1
- Moreover, C-BF down regulated the expression and secretion of TNF-α, a process involving the NF-κB signaling pathway. (unboundmedicine.com)
Inflammation3
- In intestinal inflammation, mucosal injury is often exacerbated by the reaction of NO with neutrophil-derived superoxide to form the potent oxidant peroxynitrite. (ncsu.edu)
- The intestinal barrier functions are affected by the enteric nervous system (ENS), both directly through regulation of tight junctions via neurotransmitters and neurotrophic factors [ 10 ] and indirectly through neuroimmune modulation during inflammation [ 11 ]. (hindawi.com)
- The exact role of DNMT3A in the context of intestinal inflammation has not been investigated so far. (nature.com)
Vasoactive intestinal1
- vasoactive intestinal polypeptide (VIP) , a 28-amino-acid polypeptide secreted by cells throughout the intestinal tract. (britannica.com)
Microorganisms2
- Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
- Typically, mucous membranes are coated with secretions that fight microorganisms. (msdmanuals.com)
Mucus secretion1
- It can simulate essential intestinal functions, such as nutrient absorption and mucus secretion. (moleculardevices.com)
Tract3
- In addition to the saliva, secretion of nitrate occurs at other sites in the GI tract leading to reduction by the gut flora. (inchem.org)
- The virus replicates mainly in respiratory tissues of chickens and turkeys but in the intestinal tract of clinically normal waterfowl. (thepoultrysite.com)
- This approach to the treatment of enterotoxigenic diarrhea works by interrupting the diarrhea-causing chain of events that occur when bacterial toxins enter the intestinal tract. (genengnews.com)
Inhibits1
- Peptide YY inhibits intestinal Cl- secretion in experimental porcine cryptosporidiosis through a prostaglandin-activated neural pathway. (ncsu.edu)
Organoids11
- DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)
- What are Intestinal Organoids? (stemcell.com)
- Intestinal organoids are three-dimensional (3D) in vitro tissue cultures that model the in vivo intestine. (stemcell.com)
- As the technology has matured, intestinal organoids have found application in a wide variety of research fields, including upstream drug discovery and patient-specific drug screening, cancer and immunology studies, and the pathogenesis of infectious agents. (stemcell.com)
- Since the introduction of the mouse small intestinal organoid model in 2009, 1 there has been an avalanche of developments in this field, including development of culture conditions for human organoids derived from primary colonic tissue, 2 as well as from human pluripotent stem cells (hPSCs). (stemcell.com)
- Understanding of these niche signals heavily informed development of the culture conditions for intestinal organoids. (stemcell.com)
- Most intestinal organoids are derived from primary intestinal tissue cultured in Matrigel domes with growth factors. (moleculardevices.com)
- We developed automated methods for the seeding, and media exchange, as well as monitoring the development of intestinal organoids. (moleculardevices.com)
- Watch our short webinar with Dr. Oksana Sirenko, senior scientist as she discusses the protocols used to automate 3D intestinal organoids. (moleculardevices.com)
- Intestinal organoids for automated screening assays. (moleculardevices.com)
- In this application, 3D intestinal organoids were developed from primary mouse intestinal cells cultured in Matrigel. (moleculardevices.com)
Induce1
- We know that short-chain fatty acids increase secretion of immunoglobulins, induce regulatory T-cell tissue repair, promote antimicrobial peptides and mucus production, and basically optimize intestinal function and the intestinal barrier integrity. (medscape.com)
Motility2
- The extract improved intestinal motility, increased fecal volume and normalized body weight in the constipated rats, which are indications of laxative property of the herb with the 200 mg/kg body weight of the extract showing the best efficacy. (clubalthea.com)
- Colon motility-the contraction of intestinal muscles and movement of its contents-is controlled by nerves and hormones. (healthywomen.org)
IECs1
- We found that human intestinal epithelial cells (IECs) triggered IgA(2) class switching in B cells, including IgA(1)-expressing B cells arriving from mucosal follicles, through a CD4(+) T cell-independent pathway involving a proliferation-inducing ligand (APRIL). (nih.gov)
Induces1
- In summary, PPi has a more severe effect on renal damage than MPi, as well as induces more FGF23 secretion . (bvsalud.org)
Short-chain2
- The gut microbiota can influence barrier function both directly, by stimulating epithelial cell proliferation and secretion of IL-8, and indirectly by producing short-chain fatty acids, which are an important energy source for colonic epithelial cells (colonocytes). (wikipedia.org)
- Insoluble fiber has a variety of ascribed benefits, particularly related to the changes in the gut production of short-chain fatty acids, which are incredibly important for optimizing intestinal function. (medscape.com)
Bile3
- At 90 min after drug administration, levels of unchanged drug and metabolites in plasma, intestinal contents, and bile were determined by high-performance liquid chromatography and radioactivity by liquid scintillation counting. (aspetjournals.org)
- The bile of both wild-type and mdr1a (−/−) mice contained only minor amounts of unchanged vinblastine, whereas the total biliary secretion of unknown 3 H-labeled breakdown products was about 25 to 30% of the dose. (aspetjournals.org)
- In the dog, in addition to strong salivary secretion, large quantities of nitrate were excreted in the bile following i.v. administration of nitrite, thus confirming this pathway of excretion as well as oxidation of nitrite in vivo (Walker, in press). (inchem.org)
Salivary1
- However, the kinetics of nitrate secretion in rat saliva appear to have been less well studied than in humans, and even less is known concerning salivary secretion in mice. (inchem.org)
Enteric1
- Induction of enteric oxalate secretion by Oxalobacter formigenes in mice does not require the presence of either apical oxalate transport proteins Slc26A3 or Slc26A6. (nih.gov)
Stimulation3
- Stimulation of polarized Caco-2 BBE cells with Pam2CSK4 and Pam3CSK4, agonists for TLR2/6 and TLR2/1 from the apical or basolateral sides induced secretion of IL-8 predominantly into the apical compartment regardless of the location of the stimulus, although basolateral stimulation induced smaller amounts of IL-8 compared to apical stimulation. (biomedcentral.com)
- We hypothesize that disruption of vectorial Cl transport by 5-HT may be critical in determining the alveolar flooding and abnormalities in intestinal Cl secretion through stimulation of anoctamin 1(ANO1) Cl channel. (cancertreatmentsresearch.com)
- Purpose: To find out the best location for intestinal electrical stimulation (IES) to decrease hyperglycemia, and mechanisms involving intraluminal nutrients and plasma glucagon-like peptide-1 (GLP-1) Materials and Methods: Eight rats had electrodes implanted at the duodenum and ileums for IES. (johnshopkins.edu)
Fluid6
- Intensified parasitism could not be attributed to attenuated fluid loss or changes in epithelial proliferation or replacement rate, inasmuch as iNOS inhibition did not alter severity of diarrhea, piglet hydration, Cl − secretion, or kinetics of bromodeoxyuridine-labeled enterocytes. (ncsu.edu)
- During preclinical tests, the compound was associated with a significant reduction in intestinal fluid secretion in an animal model of bacterial diarrhea. (genengnews.com)
- It caused significant decrease in fluid secretion without apparent toxicity. (genengnews.com)
- Consequently, the molecular mediators regulating the secretion of salt and fluid in the gut do not get fully activated. (genengnews.com)
- This newly discovered compound decreases the formation of ever-present cellular messenger molecules, cyclic guanosine monophosphate and cyclic adenosine monophosphate, caused by various bacterial toxins and might prevent or attenuate the intestinal fluid secretion, diarrhea, and dehydration," said Dr. Murad, the senior author of the research paper that appears in the June 16 online edition of PNAS . (genengnews.com)
- Meconium is composed of desquamated cells from the intestine and skin, gastrointestinal mucin, lanugo hair, fatty material from the vernix caseosa, amniotic fluid, and intestinal secretions. (hindawi.com)
Mice4
- We have now investigated their role in the biliary and intestinal secretion of the anticancer drugs doxorubicin (unlabeled: 5 mg/kg) and vinblastine ( 3 H-labeled: 1 mg/kg) i.v. administered to wild-type and mdr1a P-glycoprotein knockout [ mdr1a (−/−)] mice. (aspetjournals.org)
- The direct secretion of unchanged vinblastine through the gut wall was 6.7 and 3.3% of the dose in wild-type and mdr1a (−/−) mice, respectively. (aspetjournals.org)
- Approximately 10% of the dose was secreted as unchanged doxorubicin into the intestinal contents of both types of mice. (aspetjournals.org)
- This study aimed to analyze the levels of parathyroid hormone PTH and fibroblast growth factor 23 (FGF23) and the expression of renal / intestinal Pi transport-related molecules in mice fed with an MPi or PPi diet . (bvsalud.org)
Epithelial layer1
- Under these conditions, the intestinal cells self-arrange to form a single epithelial layer. (moleculardevices.com)
Determined the localization1
- Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)
Molecules1
- The effect of intestinal molecules produced by the host on the virulence of Pseudomonas fluorescens is poorly documented. (pasteur.fr)
Biliary1
- The biliary secretion of unchanged doxorubicin decreased from 13.3% of the dose to only 2.4% in the absence of mdr1a P-glycoprotein. (aspetjournals.org)
Tissue3
- However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
- Because calcium-activated chloride current in human intestinal epithelia remains controversial, we examined the localization of ANO1 in the human terminal ileum and colonic tissue using confocal microscopy. (cancertreatmentsresearch.com)
- These data demonstrate a critical role for DNMT3A in orchestrating intestinal epithelial homeostasis and response to tissue damage and suggest an involvement of impaired epithelial DNMT3A function in the etiology of IBD. (nature.com)
Context1
- 17 This property of rapid regeneration at intestinal stasis makes the intestine a uniquely convenient model system for epithelial cell biology and adult stem cell biology studies both inside and outside the specific context of intestinal function. (stemcell.com)
Villus1
- and 4) photomicrograph of intestinal villus (photograph courtesy of Alberti Lamberti, Ph.D., Temple University). (cdc.gov)
Human1
- In the present work, we evaluated the secretion of human β-defensin-2 (hBD-2) by enterocytes after infection with P. fluorescens (a species previously suggested to be involved in inflammatory bowel disease) and investigated the effect of this host-defense peptide on the bacterial virulence. (pasteur.fr)
Fecal1
- In addition, fecal exams were performed to detect intestinal parasites. (bvsalud.org)
Organoid3
- 3 Various experimental techniques have also been developed in parallel with, and applied to, intestinal organoid cultures with a scientifically synergistic effect. (stemcell.com)
- 15,16 The development of such techniques as applied to intestinal organoid cultures has vastly increased the utility of this model system for a wide variety of purposes. (stemcell.com)
- Here we demonstrate a workflow for the automation of 3D intestinal organoid culture. (moleculardevices.com)
Cytokines1
- Prenatal interaction modulated secretion of inflammatory cytokines in fetal brains, levels of mhDISC1, endogenous mouse DISC1, and glycogen synthase kinase-3beta. (researchgate.net)
Microbiome1
- Therefore, we can say that the short-chain fatty acid derivative from the intestinal microbiome does have central nervous system-related effects. (medscape.com)
Intestines1
- But the good news is that the syndrome does not cause permanent harm to the intestines, doesn't lead to intestinal bleeding and doesn't cause cancer or inflammatory bowel diseases (such as Crohn's disease or ulcerative colitis). (healthywomen.org)
Inflammatory2
- Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)
- Intestinal mucosal barrier dysfunction has been implicated in numerous health conditions such as: food allergies, microbial infections, irritable bowel syndrome, inflammatory bowel disease, celiac disease, metabolic syndrome, non-alcoholic fatty liver disease, diabetes, and septic shock. (wikipedia.org)
Parasites1
- One such 'noneradication outcome' was the impetus that the members of the ITFDE gave to initiating a demonstration project to control intestinal parasites among schoolchildren in Ghana. (cdc.gov)
Cells5
- Here we investigated IL-8 secretion in response to TLR signalling using intestinal Caco-2 BBE cells grown in the Transwell system. (biomedcentral.com)
- Therefore, the main objective of this study was to elucidate how iron-depleted, iron-saturated, and manganese-saturated forms of lactoferrin regulate intestinal barrier function via interactions with epithelial cells and macrophages. (springer.com)
- In conclusion, SARS-CoV2 infection resulted in intestinal enterochromaffin cells hyperplasia that could elevate 5-HT. (cancertreatmentsresearch.com)
- In children diagnosed with IBD, genome-wide DNA methylation profiling of purified intestinal epithelial cells clearly distinguishes between healthy individuals and the IBD group 8 . (nature.com)
- 18 At the base of the intestinal crypt, the ISCs are found intercalated with Paneth cells, 17 credited with much of the signaling required to maintain the stem cell niche. (stemcell.com)
Homeostasis1
- We show that DNMT3A plays a critical role in maintaining intestinal homeostasis and gut barrier function. (nature.com)
Severity1
- Infected piglets were treated daily with anti-CD18 or isotype control immunoglobulin G and euthanized at peak infection, at which time neutrophil infiltrates, lipid peroxidation, severity of infection, and intestinal barrier function were quantified. (ncsu.edu)