Insect Vectors: Insects that transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.Insects: The class Insecta, in the phylum ARTHROPODA, whose members are characterized by division into three parts: head, thorax, and abdomen. They are the dominant group of animals on earth; several hundred thousand different kinds having been described. Three orders, HEMIPTERA; DIPTERA; and SIPHONAPTERA; are of medical interest in that they cause disease in humans and animals. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p1)Hemiptera: A large order of insects characterized by having the mouth parts adapted to piercing or sucking. It is comprised of four suborders: HETEROPTERA, Auchenorrhyncha, Sternorrhyncha, and Coleorrhyncha.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Phytoplasma: A genus of minute bacteria in the family ACHOLEPLASMATACEAE that inhabit phloem sieve elements of infected PLANTS and cause symptoms such as yellowing, phyllody, and witches' brooms. Organisms lack a CELL WALL and thus are similar to MYCOPLASMA in animals. They are transmitted by over 100 species of INSECTS especially leafhoppers, planthoppers, and PSYLLIDS.Insect Proteins: Proteins found in any species of insect.Reoviridae: A family of unenveloped RNA viruses with cubic symmetry. The twelve genera include ORTHOREOVIRUS; ORBIVIRUS; COLTIVIRUS; ROTAVIRUS; Aquareovirus, Cypovirus, Phytoreovirus, Fijivirus, Seadornavirus, Idnoreovirus, Mycoreovirus, and Oryzavirus.Insect Control: The reduction or regulation of the population of noxious, destructive, or dangerous insects through chemical, biological, or other means.Disease Vectors: Invertebrates or non-human vertebrates which transmit infective organisms from one host to another.Ceratopogonidae: A family of biting midges, in the order DIPTERA. It includes the genus Culicoides which transmits filarial parasites pathogenic to man and other primates.Luteoviridae: A family of RNA plant viruses infecting disparate plant families. They are transmitted by specific aphid vectors. There are three genera: LUTEOVIRUS; Polerovirus; and Enamovirus.Tenuivirus: A genus of RNA plant viruses as yet unassigned to any family. Plant hosts are all in the family Poaceae. Each species is transmitted by a particular species of planthopper. The type species is Rice stripe virus.Rhodnius: A genus of the subfamily TRIATOMINAE. Rhodnius prolixus is a vector for TRYPANOSOMA CRUZI.Plant Diseases: Diseases of plants.Trypanosoma cruzi: The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.Spiroplasma citri: The type species of gram-negative bacteria in the genus SPIROPLASMA, family SPIROPLASMATACEAE, causing citrus stubborn disease.Triatominae: A subfamily of assassin bugs (REDUVIIDAE) that are obligate blood-suckers of vertebrates. Included are the genera TRIATOMA; RHODNIUS; and PANSTRONGYLUS, which are vectors of TRYPANOSOMA CRUZI, the agent of CHAGAS DISEASE in humans.Vinca: A plant genus of the family APOCYNACEAE. Vinca rosea has been changed to CATHARANTHUS roseus.Xylella: A genus of gram-negative, aerobic bacteria, in the family XANTHOMONADACEAE. It is found in the xylem of plant tissue.Plant Viruses: Viruses parasitic on plants higher than bacteria.Psychodidae: Small, hairy, moth-like flies which are of considerable public health importance as vectors of certain pathogenic organisms. Important disease-related genera are PHLEBOTOMUS, Lutzomyia, and Sergentomyia.Triatoma: A genus of the subfamily TRIATOMINAE. Several species are vectors of TRYPANOSOMA CRUZI.Host-Parasite Interactions: The relationship between an invertebrate and another organism (the host), one of which lives at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.Chagas Disease: Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.Aphids: A family (Aphididae) of small insects, in the suborder Sternorrhyncha, that suck the juices of plants. Important genera include Schizaphis and Myzus. The latter is known to carry more than 100 virus diseases between plants.Tospovirus: A genus of plant viruses in the family BUNYAVIRIDAE. Tomato spotted wilt virus is the type species. Transmission occurs by at least nine species of thrips.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Tsetse Flies: Bloodsucking flies of the genus Glossina, found primarily in equatorial Africa. Several species are intermediate hosts of trypanosomes.Aedes: A genus of mosquitoes (CULICIDAE) frequently found in tropical and subtropical regions. YELLOW FEVER and DENGUE are two of the diseases that can be transmitted by species of this genus.Trypanosomatina: A suborder of monoflagellate parasitic protozoa that lives in the blood and tissues of man and animals. Representative genera include: Blastocrithidia, Leptomonas, CRITHIDIA, Herpetomonas, LEISHMANIA, Phytomonas, and TRYPANOSOMA. Species of this suborder may exist in two or more morphologic stages formerly named after genera exemplifying these forms - amastigote (LEISHMANIA), choanomastigote (CRITHIDIA), promastigote (Leptomonas), opisthomastigote (Herpetomonas), epimastigote (Blastocrithidia), and trypomastigote (TRYPANOSOMA).Trypanosoma: A genus of flagellate protozoans found in the blood and lymph of vertebrates and invertebrates, both hosts being required to complete the life cycle.Trypanosoma brucei brucei: A hemoflagellate subspecies of parasitic protozoa that causes nagana in domestic and game animals in Africa. It apparently does not infect humans. It is transmitted by bites of tsetse flies (Glossina).Pest Control, Biological: Use of naturally-occuring or genetically-engineered organisms to reduce or eliminate populations of pests.Begomovirus: A genus of plant viruses in the family GEMINIVIRIDAE that are transmitted in nature by whitefly Bemisia tabaci.Insect Viruses: Viruses infecting insects, the largest family being BACULOVIRIDAE.Anopheles: A genus of mosquitoes (CULICIDAE) that are known vectors of MALARIA.Oryza sativa: Annual cereal grass of the family POACEAE and its edible starchy grain, rice, which is the staple food of roughly one-half of the world's population.Salivary Glands: Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).Life Cycle Stages: The continuous sequence of changes undergone by living organisms during the post-embryonic developmental process, such as metamorphosis in insects and amphibians. This includes the developmental stages of apicomplexans such as the malarial parasite, PLASMODIUM FALCIPARUM.Host-Pathogen Interactions: The interactions between a host and a pathogen, usually resulting in disease.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genes, Insect: The functional hereditary units of INSECTS.Vitis: A plant genus in the family VITACEAE, order Rhamnales, subclass Rosidae. It is a woody vine cultivated worldwide. It is best known for grapes, the edible fruit and used to make WINE and raisins.Moths: Insects of the suborder Heterocera of the order LEPIDOPTERA.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Leishmania: A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi.Leishmania mexicana: A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals including rodents. The Leishmania mexicana complex causes both cutaneous (LEISHMANIASIS, CUTANEOUS) and diffuse cutaneous leishmaniasis (LEISHMANIASIS, DIFFUSE CUTANEOUS) and includes the subspecies amazonensis, garnhami, mexicana, pifanoi, and venezuelensis. L. m. mexicana causes chiclero ulcer, a form of cutaneous leishmaniasis (LEISHMANIASIS, CUTANEOUS) in the New World. The sandfly, Lutzomyia, appears to be the vector.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Genes, Protozoan: The functional hereditary units of protozoa.Protozoan Proteins: Proteins found in any species of protozoan.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Leishmania donovani: A parasitic hemoflagellate of the subgenus Leishmania leishmania that infects man and animals and causes visceral leishmaniasis (LEISHMANIASIS, VISCERAL). The sandfly genera Phlebotomus and Lutzomyia are the vectors.Insect Repellents: Substances causing insects to turn away from them or reject them as food.Spodoptera: A genus of owlet moths of the family Noctuidae. These insects are used in molecular biology studies during all stages of their life cycle.Baculoviridae: Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.Larva: Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.Insect Hormones: Hormones secreted by insects. They influence their growth and development. Also synthetic substances that act like insect hormones.Genome, Insect: The genetic complement of an insect (INSECTS) as represented in its DNA.Lepidoptera: A large order of insects comprising the butterflies and moths.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Transduction, Genetic: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.Beetles: INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.Insect Bites and Stings: Bites and stings inflicted by insects.Diptera: An order of the class Insecta. Wings, when present, number two and distinguish Diptera from other so-called flies, while the halteres, or reduced hindwings, separate Diptera from other insects with one pair of wings. The order includes the families Calliphoridae, Oestridae, Phoridae, SARCOPHAGIDAE, Scatophagidae, Sciaridae, SIMULIIDAE, Tabanidae, Therevidae, Trypetidae, CERATOPOGONIDAE; CHIRONOMIDAE; CULICIDAE; DROSOPHILIDAE; GLOSSINIDAE; MUSCIDAE; TEPHRITIDAE; and PSYCHODIDAE. The larval form of Diptera species are called maggots (see LARVA).Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Dependovirus: A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.Lentivirus: A genus of the family RETROVIRIDAE consisting of non-oncogenic retroviruses that produce multi-organ diseases characterized by long incubation periods and persistent infection. Lentiviruses are unique in that they contain open reading frames (ORFs) between the pol and env genes and in the 3' env region. Five serogroups are recognized, reflecting the mammalian hosts with which they are associated. HIV-1 is the type species.Hemolymph: The blood/lymphlike nutrient fluid of some invertebrates.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Arthropod Vectors: Arthropods, other than insects and arachnids, which transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.Insecticides: Pesticides designed to control insects that are harmful to man. The insects may be directly harmful, as those acting as disease vectors, or indirectly harmful, as destroyers of crops, food products, or textile fabrics.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Cockroaches: Insects of the order Dictyoptera comprising several families including Blaberidae, BLATTELLIDAE, Blattidae (containing the American cockroach PERIPLANETA americana), Cryptocercidae, and Polyphagidae.Transgenes: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.Grasshoppers: Plant-eating orthopterans having hindlegs adapted for jumping. There are two main families: Acrididae and Romaleidae. Some of the more common genera are: Melanoplus, the most common grasshopper; Conocephalus, the eastern meadow grasshopper; and Pterophylla, the true katydid.Heteroptera: A suborder of HEMIPTERA, called true bugs, characterized by the possession of two pairs of wings. It includes the medically important families CIMICIDAE and REDUVIIDAE. (From Dorland, 28th ed)Support Vector Machines: Learning algorithms which are a set of related supervised computer learning methods that analyze data and recognize patterns, and used for classification and regression analysis.Tenebrio: A genus of beetles which infests grain products. Its larva is called mealworm.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Flight, Animal: The use of wings or wing-like appendages to remain aloft and move through the air.Bees: Insect members of the superfamily Apoidea, found almost everywhere, particularly on flowers. About 3500 species occur in North America. They differ from most WASPS in that their young are fed honey and pollen rather than animal food.Tribolium: A genus of small beetles of the family Tenebrionidae; T. confusum is the "confused flour beetle".Recombinant Proteins: Proteins prepared by recombinant DNA technology.Wasps: Any of numerous winged hymenopterous insects of social as well as solitary habits and having formidable stings.Periplaneta: A genus in the family Blattidae containing several species, the most common being P. americana, the American cockroach.Culicidae: A family of the order DIPTERA that comprises the mosquitoes. The larval stages are aquatic, and the adults can be recognized by the characteristic WINGS, ANIMAL venation, the scales along the wing veins, and the long proboscis. Many species are of particular medical importance.Bombyx: A genus of silkworm MOTHS in the family Bombycidae of the order LEPIDOPTERA. The family contains a single species, Bombyx mori from the Greek for silkworm + mulberry tree (on which it feeds). A native of Asia, it is sometimes reared in this country. It has long been raised for its SILK and after centuries of domestication it probably does not exist in nature. It is used extensively in experimental GENETICS. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p519)Hymenoptera: An extensive order of highly specialized insects including bees, wasps, and ants.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Anopheles gambiae: A species of mosquito in the genus Anopheles and the principle vector of MALARIA in Africa.Pupa: An inactive stage between the larval and adult stages in the life cycle of insects.Gryllidae: The family Gryllidae consists of the common house cricket, Acheta domesticus, which is used in neurological and physiological studies. Other genera include Gryllotalpa (mole cricket); Gryllus (field cricket); and Oecanthus (tree cricket).Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Ants: Insects of the family Formicidae, very common and widespread, probably the most successful of all the insect groups. All ants are social insects, and most colonies contain three castes, queens, males, and workers. Their habits are often very elaborate and a great many studies have been made of ant behavior. Ants produce a number of secretions that function in offense, defense, and communication. (From Borror, et al., An Introduction to the Study of Insects, 4th ed, p676)Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Juvenile Hormones: Compounds, either natural or synthetic, which block development of the growing insect.Insecticide Resistance: The development by insects of resistance to insecticides.Feeding Behavior: Behavioral responses or sequences associated with eating including modes of feeding, rhythmic patterns of eating, and time intervals.Weevils: BEETLES in the family Curculionidae and the largest family in the order COLEOPTERA. They have a markedly convex shape and many are considered pests.Drosophila melanogaster: A species of fruit fly much used in genetics because of the large size of its chromosomes.Orthoptera: An order of insects comprising two suborders: Caelifera and Ensifera. They consist of GRASSHOPPERS, locusts, and crickets (GRYLLIDAE).Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Mosquito Control: The reduction or regulation of the population of mosquitoes through chemical, biological, or other means.Herbivory: The act of feeding on plants by animals.Culex: A genus of mosquitoes (CULICIDAE) commonly found in tropical regions. Species of this genus are vectors for ST. LOUIS ENCEPHALITIS as well as many other diseases of man and domestic and wild animals.Arthropods: Members of the phylum Arthropoda, composed of organisms having a hard, jointed exoskeleton and paired jointed legs. It includes the class INSECTS and the subclass ARACHNIDA, many species of which are important medically as parasites or as vectors of organisms capable of causing disease in man.Nymph: The immature stage in the life cycle of those orders of insects characterized by gradual metamorphosis, in which the young resemble the imago in general form of body, including compound eyes and external wings; also the 8-legged stage of mites and ticks that follows the first moult.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Arachnid Vectors: Members of the class Arachnida, especially SPIDERS; SCORPIONS; MITES; and TICKS; which transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Photorhabdus: A genus of gram-negative bacteria existing symbiotically with nematodes of the family Heterorhabditidae (see RHABDITOIDEA). These nematodes infect a variety of soil-dwelling insects. Upon entering an insect host, the nematode releases Photorhabdus from its intestinal tract and the bacterium establishes a lethal septicemia in the insect.Houseflies: Flies of the species Musca domestica (family MUSCIDAE), which infest human habitations throughout the world and often act as carriers of pathogenic organisms.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Helper Viruses: Viruses which enable defective viruses to replicate or to form a protein coat by complementing the missing gene function of the defective (satellite) virus. Helper and satellite may be of the same or different genus.Molting: Periodic casting off FEATHERS; HAIR; or cuticle. Molting is a process of sloughing or desquamation, especially the shedding of an outer covering and the development of a new one. This phenomenon permits growth in ARTHROPODS, skin renewal in AMPHIBIANS and REPTILES, and the shedding of winter coats in BIRDS and MAMMALS.Oviposition: The process of laying or shedding fully developed eggs (OVA) from the female body. The term is usually used for certain INSECTS or FISHES with an organ called ovipositor where eggs are stored or deposited before expulsion from the body.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Luminescent Proteins: Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Beauveria: A mitosporic fungal genus. Teleomorphs are found in the family Clavicipitaceae and include Cordyceps bassiana. The species Beauveria bassiana is a common pathogen of ARTHROPODS and is used in PEST CONTROL.DEET: A compound used as a topical insect repellent that may cause irritation to eyes and mucous membranes, but not to the skin.Xenorhabdus: A genus of gram-negative, facultatively anaerobic rod-shaped cells which are motile by peritrichous flagella. Late in the growth cycle, spheroplasts or coccoid bodies occur, resulting from disintegration of the cell wall. The natural habitat is the intestinal lumen of certain nematodes. (From Bergey's Manual of Determinative Bacteriology, 9th ed)beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Nucleopolyhedrovirus: A genus of the family BACULOVIRIDAE, subfamily Eubaculovirinae, characterized by the formation of crystalline, polyhedral occlusion bodies in the host cell nucleus. The type species is Autographa californica nucleopolyhedrovirus.Fat Body: A nutritional reservoir of fatty tissue found mainly in insects and amphibians.Wolbachia: A genus of bacteria comprised of a heterogenous group of gram-negative small rods and coccoid forms associated with arthropods. (From Bergey's Manual of Systematic Bacteriology, vol 1, 1984)Metarhizium: A mitosporic fungal genus in the family Clavicipitaceae. It has teleomorphs in the family Nectriaceae. Metarhizium anisopliae is used in PESTICIDES.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Genes, Reporter: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.Hemocytes: Any blood or formed element especially in invertebrates.Adenovirus E1 Proteins: The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Malpighian Tubules: Slender tubular or hairlike excretory structures found in insects. They emerge from the alimentary canal between the mesenteron (midgut) and the proctodeum (hindgut).Bacillus thuringiensis: A species of gram-positive bacteria which may be pathogenic for certain insects. It is used for the biological control of the Gypsy moth.Biological Evolution: The process of cumulative change over successive generations through which organisms acquire their distinguishing morphological and physiological characteristics.Metamorphosis, Biological: Profound physical changes during maturation of living organisms from the immature forms to the adult forms, such as from TADPOLES to frogs; caterpillars to BUTTERFLIES.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.Arthropod Antennae: Paired sense organs connected to the anterior segments of ARTHROPODS that help them navigate through the environment.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Pyrethrins: The active insecticidal constituent of CHRYSANTHEMUM CINERARIIFOLIUM flowers. Pyrethrin I is the pyretholone ester of chrysanthemummonocarboxylic acid and pyrethrin II is the pyretholone ester of chrysanthemumdicarboxylic acid monomethyl ester.Malaria: A protozoan disease caused in humans by four species of the PLASMODIUM genus: PLASMODIUM FALCIPARUM; PLASMODIUM VIVAX; PLASMODIUM OVALE; and PLASMODIUM MALARIAE; and transmitted by the bite of an infected female mosquito of the genus ANOPHELES. Malaria is endemic in parts of Asia, Africa, Central and South America, Oceania, and certain Caribbean islands. It is characterized by extreme exhaustion associated with paroxysms of high FEVER; SWEATING; shaking CHILLS; and ANEMIA. Malaria in ANIMALS is caused by other species of plasmodia.Sf9 Cells: Cell line derived from SF21 CELLS which are a cell line isolated from primary explants of SPODOPTERA FRUGIPERDA pupal tissue.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Population Density: Number of individuals in a population relative to space.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Ecdysterone: A steroid hormone that regulates the processes of MOLTING or ecdysis in insects. Ecdysterone is the 20-hydroxylated ECDYSONE.Ecdysteroids: Steroids that bring about MOLTING or ecdysis in insects. Ecdysteroids include the endogenous insect hormones (ECDYSONE and ECDYSTERONE) and the insect-molting hormones found in plants, the phytoecdysteroids. Phytoecdysteroids are natural insecticides.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.WingMutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Locusta migratoria: A species of migratory Old World locusts, in the family ACRIDIDAE, that are important pests in Africa and Asia.Integumentary System: The outer covering of the body composed of the SKIN and the skin appendages, which are the HAIR, the NAILS; and the SEBACEOUS GLANDS and the SWEAT GLANDS and their ducts.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Butterflies: Slender-bodies diurnal insects having large, broad wings often strikingly colored and patterned.Ecosystem: A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Gene Targeting: The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.Adenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.RNA Interference: A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.

*  Bridging the gaps in vector biology | EMBO Reports

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*  Insect Vector Unit goes in | The Trinidad Guardian Newspaper

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*  Insect pests and insect-vectored diseases of palms - a review - Australian Museum

This paper discusses insect pests that attack palms, pathogens the insects vector as well as other disorders that are ... Insect pests and insect-vectored diseases of palms - a review. Australian Journal of Entomology. 48. 328-342. ... Refereed Article Insect pests and insect-vectored diseases of palms - a review ... Palm production faces serious challenges ranging from diseases to damage by insect pests, all of which may reduce productivity ...

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*  Spatial and temporal distribution of insect vectors of Xanthomonas campestris pv. musacearum and their activity across banana...

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Rakiura (genus): Rakiura is a genus of Trichoptera (caddisfly). The genus contains only one species, R.Silverleaf whiteflyMultiple cloning site: A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites - a standard feature of engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid.Sandal spike phytoplasma: Sandal (Santalum album. L), a semi-root parasitic tree is the source of the East Indian sandalwood and oil.Ditch: A ditch is a small to moderate depression created to channel water. A ditch can be used for drainage, to drain water from low-lying areas, alongside roadways or fields, or to channel water from a more distant source for plant irrigation.Culicoides imicola: Culicoides imicola is a midge which transmits the bluetongue virus. Other suspected BTV vectors are Culicoides (Culicoides) pulicaris and species in the Culicoides (Avaritia) obsoletus complex.Rice hoja blanca virus: Rice hoja blanca virus (RHBV) is the agent of the most important viral disease of rice in Latin America.Morales, F.Rhodnius nasutus: Rhodnius nasutus is a Chagas disease vector native to the northeast of Brazil. It is primarily associated with the Copernicia prunifera palm tree (Carnauba).Fungicide use in the United States: A more accurate title for this page would be "Common plant pathogens to food crops in the United States".Trypanosoma: Trypanosoma is a genus of kinetoplastids (class Kinetoplastida), a monophyletic group of unicellular parasitic flagellate protozoa. The name is derived from the Greek trypano- (borer) and soma (body) because of their corkscrew-like motion.Colley Hill: Colley Hill is part of the North Downs escarpment in the North Downs, Surrey, England. It is about 1 km east of Buckland Hills and 1 km west of Reigate Hill, all of which form part of the same escarpment.Dipetalogaster: Dipetalogaster, a genus of Triatominae, the kissing bugs, has only a single species, Dipetalogaster maxima, which is found in the Mexican state of Baja California Sur. The blood-sucking Dipetalogaster live in crevices in the rocks and feed on lizards.Matthiola incana: Matthiola incana, known as hoary stock, is a species of flowering plant in the genus Matthiola. The common name stock usually refers to this species, though it may also be applied to the whole genus.Leaf scorch: Leaf scorch (also called leaf burn, leaf wilt, and sun scorch) is defined as a browning of plant tissues, including leaf margins and tips, and yellowing or darkening of veins which may lead to eventual wilting and abscission of the leaf.Wound tumor virus: Wound tumor virus is an invertebrate and plant virus found in the United States of America belonging to the genus Phytoreovirus and the family Reoviridae. The virus is a Type III virus under the Baltimore classification system; that is it has a double-stranded RNA genome.Adolfo Lutz: Adolfo Lutz (6 October 1855 – 18 December 1940) was a Brazilian physician, father of tropical medicine and medical zoology in Brazil, and a pioneer epidemiologist and researcher in infectious diseases.Triatoma infestansChagas: Time to Treat campaign: The Chagas: Time to Treat Campaign is an international campaign started by the Drugs for Neglected Diseases initiative to advocate for increased research and development of treatments for Chagas disease. Chagas is a potentially fatal neglected disease that affects between 8 and 13 million people worldwide.Acyrthosiphon pisum: Acyrthosiphon pisum, commonly known as the pea aphid (and colloquially known as the green dolphin, pea louse, and clover louse ), is a sap-sucking insect in the Aphididae family. It feeds on several species of legumes (plant family Fabaceae) worldwide, including forage crops, such as pea, clover, alfalfa, and broad bean, and ranks among the aphid species of major agronomical importance.Coles PhillipsHaematozoa: Haematozoa or Hematozoa is a general term that includes blood parasites, mainly protozoans. Well known examples include the malaria and trypanosoma parasites, but a large number of species are known to infect birds and are transmitted by arthropod vectors.Aedes aegyptiTrypanosoma equiperdum: Τrypanosoma equiperdum is a protozoan parasite that causes Dourine or covering sickness in horses and other animals in the family equidae. Genetic analyses have revealed T.Trypanosoma bruceiBiopesticide: Biopesticides, a contraction of 'biological pesticides', include several types of pest management intervention: through predatory, parasitic, or chemical relationships. The term has been associated historically with biological control - and by implication - the manipulation of living organisms.Bakhtawar Amin Memorial Hospital Multan: Bakhtawar_Amin_Memorial_Hospital_Multan is a hospital located in Multan, Pakistan.PolyhedrinAnopheles culicifacies: Anopheles culicifacies (sensu lato) is one of the major vectors of malaria on the Indian Subcontinent. It has been reported to be a species complex consisting of five sibling species which have been provisionally designated as species A, B, C, D, and E.Weedy rice: Weedy rice, also known as red rice, is a variety of rice (Oryza) that produces far fewer grains per plant than cultivated rice and is therefore considered a pest. The name "weedy rice" is used for all types and variations of rice which show some characteristic features of cultivated rice and grow as weeds in commercial rice fields.Dredge turning gland: Dredge Turning Gland is a Trailing Suction Hopper Dredger component.Infrastructure Lifecycle Management: Infrastructure Lifecycle Management (ILM) is a term coined by the real estate sector. It covers the management of all core processes around planning, construction, operation, maintenance and commercialization of buildings or property.Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Viticulture: Viticulture (from the Latin word for vine) is the science, production, and study of grapes. It deals with the series of events that occur in the vineyard.Antheraea pernyi: Antheraea pernyi, the Chinese (Oak) tussah moth (or "Chinese tasar moth"), also known as temperate tussah moth, is a large moth in the family Saturniidae. Antheraea roylei is an extremely close relative, and the present species might actually have evolved from ancestral A.Branching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.Leishmania aethiopica: Leishmania aethiopica is a Leishmania species.Leishmania mexicanaSymbiosis Center of Health Care: Symbiosis Center of Health Care (SCHC) is an organization under Symbiosis Society which takes care of health of symbiosis family be it student or staff.http://www.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.Microneme: Micronemes are cellular organs, or organelles, possessed by Apicomplexa protozoans that are restricted to the apical third of the protozoan body. They are surrounded by a typical unit membrane.DNA sequencer: A DNA sequencer is a scientific instrument used to automate the DNA sequencing process. Given a sample of DNA, a DNA sequencer is used to determine the order of the four bases: G (guanine), C (cytosine), A (adenine) and T (thymine).Leishmania donovani: Leishmania donovani is a species of intracellular parasitic protozoan belonging to the genus Leishmania, a group of haemoflagellate kinetoplastids that cause the disease leishmaniasis. It is a human blood parasite responsible for visceral leishmaniasis or kala-azar, the most severe form of leishmaniasis.RID Insect Repellent: RID is an Australian brand of personal insect repellent sold and distributed in Australia, New Zealand, worldwide and online.BacMamAndesobia jelskiiProthoracicotropic hormone: Prothoracicotropic hormone (PTTH) was the first insect hormone to be discovered. It was originally described simply as "brain hormone" by early workers such as Stefan Kopeć (1922) and Vincent Wigglesworth (1934),Wigglesworth, V.Thermal cyclerAdalia bipunctata: Adalia bipunctata, commonly known as the two-spot ladybird, two-spotted ladybug or two-spotted lady beetle, is a carnivorous beetle of the family Coccinellidae that is found throughout the holarctic region. It is very common in western and central Europe.Insect sting allergy: Insect sting allergy is the term commonly given to the allergic response of an animal in response to the bite or sting of an insect. Typically, insects which generate allergic responses are either stinging insects (wasps, bees, hornets and ants) or biting insects (mosquitoes, ticks).Chrysomya albiceps: Chrysomya albiceps is a species belonging to the blow fly family, Calliphoridae. It is of great medical and sanitary importance, being associated with myiasis in Africa and America although it plays a more significant role as a predator of other dipteran larvae.Self-complementary adeno-associated virus (scAAV): Self-complementary adeno-associated virus (scAAV) is a viral vector engineered from the naturally occurring adeno-associated virus (AAV) to be used as a tool for gene therapy. Use of recombinant AAV (rAAV) has been successful in clinical trials addressing a variety of diseases.Hemolymph: Hemolymph, or haemolymph, is a fluid, analogous to the blood in vertebrates, that circulates in the interior of the arthropod body remaining in direct contact with the animal's tissues. It is composed of a fluid plasma in which hemolymph cells called hemocytes are suspended.Ligation-independent cloning: Ligation-independent cloning (LIC) is a form of molecular cloning that is able to be performed without the use of restriction endonucleases or DNA ligase. This allows genes that have restriction sites to be cloned without worry of chopping up the insert.Insecticide: An insecticide is a substance used to kill insects. They include ovicides and larvicides used against insect eggs and larvae, respectively.Depopulation of cockroaches in post-Soviet states: A mass depopulation of cockroaches has been observed since the beginning of the 21st century in Russia and other countries of the former USSR. Observers note quick disappearance of various types of cockroaches from cities and towns in Russia, Azerbaijan, Kazakhstan, Ukraine, Moldova, and Belarus.List of locust species: This is a list of locust species. These are Orthoptera that swarm and cause significant economic losses to agricultural crops.Strider Knives: Strider Knives, Inc. is a custom and production knifemaking facility headed by Mick Strider and Duane Dwyer based in San Marcos, California.Corinna CortesMealwormRNA transfection: RNA transfection is the process of deliberately introducing RNA into a living cell. RNA can be purified from cells after lysis or synthesized from free nucleotides either chemically, or enzymatically using an RNA polymerase to transcribe a DNA template.Harmening High Flyer: The Harmening High Flyer is an American powered parachute that was designed and produced by Harmening's High Flyers of Genoa, Illinois.Cliche, Andre: Ultralight Aircraft Shopper's Guide 8th Edition, page D-7.List of diseases of the honey bee: Diseases of the honey bee or abnormal hive conditions include:Polistes dominula: The European paper wasp (Polistes dominula, often misspelled as dominulus) is one of the more common and well-known species of social wasps in the genus Polistes. Its diet is more diverse than that of most Polistes species (many genera of insects versus mainly caterpillars in other Polistes), making it superior over many others during the shortage of resources.Periplanone BPsorophora howardiiCynthia (Gaba girl): Cynthia was a plaster mannequin of the 1930s created by Lester Gaba.Larch sawfly: The larch sawfly (Pristiphora erichsonii) is a species of sawfly. The adult sawfly resembles a black wasp, is about ½ inch in length with a thick waist and has brown to orangish markings on the abdomen.Teleogryllus oceanicus: Teleogryllus oceanicus, commonly known as the Australian, Pacific or oceanic field cricket, is a cricket found across Oceania and in coastal Australia from Carnarvon in Western Australia and Rockhampton in north-east Queensland Otte, D. & Alexander, R.Synapto-pHluorin: Synapto-pHluorin is a genetically encoded optical indicator of vesicle release and recycling. It is used in neuroscience to study transmitter release.Camponotus vagus: Camponotus vagus is a species of large, black, West Palaearctic carpenter ant with a wide range that includes much of Europe, a large area of Asia, and part of Africa.Norwegian Journal of EntomologyEncyclopedia of Life: Camponotus vagus (Scopoli, 1763)Chromosome engineering: Chromosome engineering is "the controlled generation of chromosomal deletions, inversions, or translocations with defined endpoints." For: By combining chromosomal translocation, chromosomal inversion,and chromosomal deletion, chromosome engineering has been shown to identify the underlying genes that cause certain diseases in mice.Juvenile hormone: Juvenile hormones (JHs) are a group of acyclic sesquiterpenoids that regulate many aspects of insect physiology. JHs regulate development, reproduction, diapause, and polyphenisms.

(1/3745) Can anything be done to maintain the effectiveness of pyrethroid-impregnated bednets against malaria vectors?

Pyrethroid-treated bednets are the most promising available method of controlling malaria in the tropical world. Every effort should be made to find methods of responding to, or preventing, the emergence of pyrethroid resistance in the Anopheles vectors. Some cases of such resistance are known, notably in An. gambiae in West Africa where the kdr type of resistance has been selected, probably because of the use of pyrethroids on cotton. Because pyrethroids are irritant to mosquitoes, laboratory studies on the impact of, and selection for, resistance need to be conducted with free-flying mosquitoes in conditions that are as realistic as possible. Such studies are beginning to suggest that, although there is cross-resistance to all pyrethroids, some treatments are less likely to select for resistance than others are. Organophosphate, carbamate and phenyl pyrazole insecticides have been tested as alternative treatments for nets or curtains. Attempts have been made to mix an insect growth regulator and a pyrethroid on netting to sterilize pyrethroid-resistant mosquitoes that are not killed after contact with the netting. There seems to be no easy solution to the problem of pyrethroid resistance management, but further research is urgently needed.  (+info)

(2/3745) Mayaro virus disease: an emerging mosquito-borne zoonosis in tropical South America.

This report describes the clinical, laboratory, and epidemiological findings on 27 cases of Mayaro virus (MV) disease, an emerging mosquito-borne viral illness that is endemic in rural areas of tropical South America. MV disease is a nonfatal, dengue-like illness characterized by fever, chills, headache, eye pain, generalized myalgia, arthralgia, diarrhea, vomiting, and rash of 3-5 days' duration. Severe joint pain is a prominent feature of this illness; the arthralgia sometimes persists for months and can be quite incapacitating. Cases of two visitors from the United States, who developed MV disease during visits to eastern Peru, are reported. MV disease and dengue are difficult to differentiate clinically.  (+info)

(3/3745) Vectors of Chikungunya virus in Senegal: current data and transmission cycles.

Chikungunya fever is a viral disease transmitted to human beings by Aedes genus mosquitoes. From 1972 to 1986 in Kedougou, Senegal, 178 Chikungunya virus strains were isolated from gallery forest mosquitoes, with most of them isolated from Ae. furcifer-taylori (129 strains), Ae. luteocephalus (27 strains), and Ae. dalzieli (12 strains). The characteristics of the sylvatic transmission cycle are a circulation periodicity with silent intervals that last approximately three years. Few epidemics of this disease have been reported in Senegal. The most recent one occurred in 1996 in Kaffrine where two Chikungunya virus strains were isolated from Ae. aegypti. The retrospective analysis of viral isolates from mosquitoes, wild vertebrates, and humans allowed to us to characterize Chikungunya virus transmission cycles in Senegal and to compare them with those of yellow fever virus.  (+info)

(4/3745) Isolation of tick-borne encephalitis virus from wild rodents and a seroepizootiologic survey in Hokkaido, Japan.

To determine the vertebrate host of tick-borne encephalitis (TBE) virus in the southern part of Hokkaido, Japan, virus isolation was performed using spleens from small mammals captured in the area. Two virus strains were isolated, one strain from Apodemus speciosus and another from Clethrionomys rufocanus. Virus isolates were inoculated onto baby hamster kidney cell monolayers and antigen slides were prepared for an indirect immunofluorescent antibody assay. Two isolates were identified as TBE viruses by monoclonal antibody reactions. To specify the TBE-endemic area in Hokkaido, rodent, horse, and dog sera collected from 1992 to 1997 were tested for neutralization antibody against TBE virus previously isolated from a dog. The positive cases were distributed in four districts in the southern part of Hokkaido.  (+info)

(5/3745) Variation in oral susceptibility to dengue type 2 virus of populations of Aedes aegypti from the islands of Tahiti and Moorea, French Polynesia.

Twenty three samples of Aedes aegypti populations from the islands of Tahiti and Moorea (French Polynesia) were tested for their oral susceptibility to dengue type 2 virus. The high infection rates obtained suggest that the artificial feeding protocol used was more efficient than those previously described. Statistical analysis of the results allowed us to define two distinct geographic areas on Tahiti with respect to the susceptibility of Ae. aegypti: the east coast, with homogeneous infection rates, and the west coast, with heterogeneous infection rates. No geographic differences could be demonstrated on Moorea. The possible mechanisms of this phenomenon are discussed in connection with recent findings on the variability of susceptibility of Ae. aegypti to insecticides.  (+info)

(6/3745) Replication of dengue type 2 virus in Culex quinquefasciatus (Diptera: Culicidae).

We were able to infect Culex quinquefasciatus by the parenteral route with dengue virus type 2. The percentage of mosquitoes infected was dose dependent and we obtained a rate of 45.6% infected Cx. quinquefasciatus when a 10(5.9) MID50 (mosquito infectious dose for 50% of the individuals as measured in Aedes aegypti) of dengue virus type 2 per mosquito was used. Infection was detected by an immunofluorescent assay performed on mosquito head squashes 14 days after infection. The replication of dengue virus in Cx. quinquefasciatus was either at a very low level of magnitude or generated a large number of noninfectious particles since the triturated bodies of infected Cx. quinquefasciatus did not infect Ae. aegypti mosquitoes when inoculated parenterally. We were unable to infect Cx. quinquefasciatus females orally with an artificial meal that infected 100% of Ae. aegypti females. These findings lead us to agree with the consensus that Cx. quinquefasciatus should not be considered a biological vector of dengue viruses.  (+info)

(7/3745) Tissue tropism related to vector competence of Frankliniella occidentalis for tomato spotted wilt tospovirus.

The development of tomato spotted wilt tospovirus (TSWV) infection in the midgut and salivary glands of transmitting and non-transmitting thrips, Frankliniella occidentalis, was studied to elucidate tissue tropism and the virus pathway within the body of this vector. Immunohistological techniques used in this study showed that the midgut, foregut and salivary glands were the only organs in which virus accumulated. The first signals of infection, observed as randomly distributed fluorescent granular spots, were found in the epithelial cells of the midgut, mainly restricted to the anterior region. The virus subsequently spread to the circular and longitudinal midgut muscle tissues, a process which occurred late in the larval stage. In the adult stage, the infection occurred in the visceral muscle tissues, covering the whole midgut and foregut, and was abolished in the midgut epithelium. The infection of the salivary glands was first observed 72 h post-acquisition, and simultaneously in the ligaments connecting the midgut with these glands. The salivary glands of transmitting individuals appeared heavily or completely infected, while no or only a low level of infection was found in the glands of non-transmitting individuals. Moreover, the development of an age-dependent midgut barrier against virus infection was observed in second instar larvae and adults. The results show that the establishment of TSWV infection in the various tissues and the potential of transmission seems to be regulated by different barriers and processes related to the metamorphosis of thrips.  (+info)

(8/3745) A GroEL homologue from endosymbiotic bacteria of the whitefly Bemisia tabaci is implicated in the circulative transmission of tomato yellow leaf curl virus.

Evidence for the involvement of a Bemisia tabaci GroEL homologue in the transmission of tomato yellow leaf curl geminivirus (TYLCV) is presented. A approximately 63-kDa protein was identified in B. tabaci whole-body extracts using an antiserum raised against aphid Buchnera GroEL. The GroEL homologue was immunolocalized to a coccoid-shaped whitefly endosymbiont. The 30 N-terminal amino acids of the whitefly GroEL homologue showed 80% homology with that from different aphid species and GroEL from Escherichia coli. Purified GroEL from B. tabaci exhibited ultrastructural similarities to that of the endosymbiont from aphids and E. coli. In vitro ligand assays showed that tomato yellow leaf curl virus (TYLCV) particles displayed a specific affinity for the B. tabaci 63-kDa GroEL homologue. Feeding whiteflies anti-Buchnera GroEL antiserum before the acquisition of virions reduced TYLCV transmission to tomato test plants by >80%. In the haemolymph of these whiteflies, TYLCV DNA was reduced to amounts below the threshold of detection by Southern blot hybridization. Active antibodies were recovered from the insect haemolymph suggesting that by complexing the GoEL homologue, the antibody disturbed interaction with TYLCV, leading to degradation of the virus. We propose that GroEL of B. tabaci protects the virus from destruction during its passage through the haemolymph.  (+info)


  • In T. cruzi , one of the stresses experienced during the passage from one host to another is the exposure to sudden changes in temperature, while, inside the insect, the parasites are subjected to an average temperature of 26°C, but, once they enter the mammalian host, they face temperatures of 37°C or more, experiencing a heat shock [ 10 ]. (


  • Culicoides brevitarsis - sospechado ser vector de Akabane y virus Aino. (
  • Culicoides imicola - vector mayor de virus de la lengua azul y del enfermedad equina africana. (
  • Haematomyidium Culicoides insinuatus Culicoides paraensis - vector de virus Oropouche. (
  • Monoculicoides Culicoides impunctatus - vector de Haemoproteus spp. (
  • The AHS virus (AHSV) is transmitted almost exclusively by Culicoides midges (Diptera: Ceratopogonidae), of which Culicoides imicola is considered to be the most important vector. (
  • However, little is known regarding the presence of culicoides in unusual habitats such as peaÿ marshes, nor their potential vector capacity. (
  • High numbers of biting midges were captured in the marshy area and most of them were Culicoides impunc{atus, a potential vector of BïV and other pâthogens. (
  • Very high numbers of biting midges were captured in the marshy area and most of them (70 to 95%) were Culicoides impunctatus, a potential vector of BTV and other pathogens. (


  • 2009). The geographic distribution and seasonal incidence of AHSV depend not only on the presence of the virus and susceptible equines but also on the presence and abundance of competent vectors (Mellor, Boorman & Baylis 2000). (
  • In the digestive tube of the insect vector, the presence of digestive enzymes and a hemolytic factor produce a potentially hostile environment for the parasite [ 9 ]. (


  • In addition, once the parasite is inside the insect or vertebrate, it is exposed to fluctuations that occur due to the switch from the extracellular to the intracellular environment and the host immune response [ 6 ]. (