HL-60 Cells: A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)Leukemia, Promyelocytic, Acute: An acute myeloid leukemia in which abnormal PROMYELOCYTES predominate. It is frequently associated with DISSEMINATED INTRAVASCULAR COAGULATION.Dimethyl Sulfoxide: A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Tretinoin: An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Granulocytes: Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.Tetradecanoylphorbol Acetate: A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Leukemia, Myeloid: Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Calcitriol: The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)N-Formylmethionine Leucyl-Phenylalanine: A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.

*  Gentaur Molecular :Exbio \ Mouse Monoclonal to HLA-DR+DP, Clone HL-38, Isotype IgG2aApplication FC, IP, WB Concentration 1...

Clone HL-38, Isotype IgG2aApplication FC, IP, WB Concentration 1 mg_ml \ 11-239-C100 for more molecular products just contact ... WP1011: T Cell Receptor Signaling Pathway. WP1021: Phase I, non P450. WP1025: B Cell Receptor Signaling Pathway. WP1029: Notch ... WP1130: T Cell Receptor Signaling Pathway. WP1144: B Cell Receptor Signaling Pathway. WP1150: IL-3 Signaling Pathway. WP1163: ... WP1345: T Cell Receptor Signaling Pathway. WP1354: B Cell Receptor Signaling Pathway. WP1359: IL-3 Signaling Pathway. WP1388: ...

*  Arachidonic acid metabolism by nuclei of a retinoic acid--or vitamin D3-differentiated human leukemia cell line HL-60.

... cells was investigated during retinoic acid (RA)-induced granulocytic differentiation and 1 alpha, 25 dihydroxy-vitamin D3- ... Cell Differentiation / drug effects. Cell Nucleus / drug effects, metabolism. Dinoprost / biosynthesis. Dinoprostone / ... 21466279 - Schwann cells can be reprogrammed to multipotency by culture.. 21293189 - Cell migration regulates the kinetics of ... The whole control HL-60 cells and their nuclei hardly converted [1-14C]-AA to any metabolites comigrating with authentic ...

*  AID 81276 - Apoptotic activity against HL-60 (Human leukemia) cell after 6 hr of stimulation measured by MTT assay - PubChem

... cell after 6 hr of stimulation measured by MTT assay. ...

*  AID 641136 - Inhibition of human PIP5K3 in HL-60 cells lysate assessed as reduction of labeling of acyl-phosphate ATP probe at...

Inhibition of human PIP5K3 in HL-60 cells lysate assessed as reduction of labeling of acyl-phosphate ATP probe at 100 nM. ...

*  IJMS | Free Full-Text | Ethyl Gallate Induces Apoptosis of HL-60 Cells by Promoting the Expression of Caspases-8, -9, -3,...

We examined cell viability, morphological changes, DNA content and fragmentation, and expression of apoptosis-related proteins ... The results showed that EG induced morphological changes and DNA fragmentation and reduced HL-60 cell viability in a dose- ... In this study, we investigated ethyl gallate (EG) for possible proapoptotic effects in the human promyelocytic leukemia cell ... Western blotting analysis indicated that EG-mediated HL-60 apoptosis mainly occurred through the mitochondrial pathway, as ...

*  Induction of Differentiation and Cellular Manipulation of Human Myeloid HL-60 Leukemia Cells

... in differentiation and ... reprogramming of somatic cells of cell and tissue culture associated techniques ... Count at least 200 cells/data point; the percentage cell spreading = (number of spread cells/total number of adherent cells) × ... Pellet the cells by gentle (250g) centrifugation. Resuspend in fresh growth medium and plate the cells at 2-10 × 104 cells/ml ...

*  Effects of Pyrimidine Antagonists on Sialic Acid Regeneration in HL-60 Cells | Cancer Research

Because alterations in cell membrane sialoglycoconjugates can affect the behavior of neoplastic cells, we investigated the ... The idea that inhibition of membrane sialylation by chemotherapeutic drugs may alter cell surface properties of tumor cells and ... Effects of Pyrimidine Antagonists on Sialic Acid Regeneration in HL-60 Cells. Alexander A. Hindenburg, Robert N. Taub, Steven ... In these studies, cells were incubated with Vibrio cholerae neuraminidase to remove surface sialic acid. Reappearance of ...

*  Uncoupling of cell cycle arrest from the expression of monocytic differentiation markers in HL60 cell variants. - PubMed - NCBI

Exp Cell Res. 1997 May 1;232(2):376-87. Comparative Study; Research Support, U.S. Gov't, P.H.S. ... However, B series cells resembled the A series cells in exhibiting faster growth rates than the parental HL60 cells and showed ... Uncoupling of cell cycle arrest from the expression of monocytic differentiation markers in HL60 cell variants.. Studzinski GP1 ... Differentiation generally leads to cell cycle arrest. Human leukemia HL60 cells respond to the presence of 1,25- ...

*  AID 81636 - The cytotoxic activity in HL-60 cells - PubChem

BioAssay record AID 81636 submitted by ChEMBL: The cytotoxic activity in HL-60 cells.

*  AID 244024 - Inhibition of HL-60 cell adhesion to recombinant human Selectin P at 50 mM; Not active up to 100 mM - PubChem

Inhibition of HL-60 cell adhesion to recombinant human Selectin P at 50 mM; Not active up to 100 mM. ...

*  HL-60 cells | Cell-culture Database - TOKU-E

Transfection and selection data of HL-60 cells by various antibiotics in differing media. ... Cell-Lines , Plasmids , Privacy Policy , Terms of Use , About Us , Contact Us. Cell-Culture Database Version 1.1 ...

*  Basic Mechanisms of Arsenic Trioxide (ATO)-Induced Apoptosis in Human Leukemia (HL-60) Cells | Journal of Hematology & Oncology...

As seen in Figure 2, there was a gradual increase in annexin V positive cells (apoptotic cells) in ATO-treated cells compared ... cell cycle progression, programmed cell death, and cellular function in cultured HL-60 promyelocytic leukemia cells. Among ... probably due to high level of cell death. The percentages of annexin V-positive cells in ATO-treated HL-60 populations were ... Control cells were processed exactly as ATO-treated cells, except ATO treatment of these cells was eliminated. Control and ATO- ...

*  Localization of Nox2 N-terminus using polyclonal antipeptide antibodies | Biochemical Journal

The cultures of HL60 cell were initiated at 5×105 cells/ml on a weekly basis. Differentiation of HL60 cells was induced by the ... Culture and differentiation of HL60 cells. HL60 cells, a human promyelocytic cell line [30], were maintained in RPMI 1640 ... Immunocytochemistry on HL60 cells by confocal microscopy. Differentiated and undifferentiated HL60 cells were harvested by ... Immunodetection of gp91phox on differentiated HL60 cells by confocal microscopy. The incubation of the promyelocytic HL60 cells ...

*  BioSprint 96 DNA Blood Kit - QIAGEN Online Shop

Genomic DNA can be purified from cells, tissue, and blood. Lysed samples are loaded into the instrument, which then performs ... HL-60 cultured cells. 2 x 106 cells. 9.6 ± 5.6. Blood, human (5-7 x 106 cells per ml). 200 µl. 4.5-9.0. ... The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. ... For purification of DNA from human whole blood, animal whole blood, buffy coat, cultured cells, tissues, rodent tails, buccal ...

*  Anti-FER antibody - C-terminal (ab71682) | Abcam

Cell Biology. Epigenetics. Metabolism. Developmental Biology. By research area. Immunology. Microbiology. Neuroscience. Signal ... Anti-FER antibody - C-terminal (ab71682) at 1/100 dilution + HL-60 cell lysate at 12.5 µg. Predicted band size : 93 kDa. ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ... Probably performs an important function, perhaps in regulatory processes such as cell cycle control. ...

*  Patent US5037816 - Method of treating psoriasis - Google Patents

... patient an effective amount of a vitamin D compound which is capable of stimulating the differentiation of cultured tumor cells ... The method of claim 10 wherein said human tumor cells are selected from the group consisting of:. (a) HL-60 cells, and ... When psoriatic cells were incubated with 1,25-(OH)2 -D3 at 10-6 M, there was a small but significant effect on inhibiting cell ... 4) the cell size and cell density, or. (5) morphological changes seen under a light microscope, or some or all of the above in ...

*  NIOSHTIC-2 Publications Search - 20031866 - Nitrosative stress inhibits the aminophospholipid translocase resulting in...

Macrophage recognition of apoptotic cells depends on externalization of phosphatidylserine (PS), which is normally maintained ... Blood-cells; Blood-disorders; Immune-system-disorders; Cell-alteration; Cell-metabolism; Cell-function; Cell-biology; Amino- ... Here we report that exposure of target HL-60 cells to nitrosative stress inhibited APLT, induced PS externalization, and ... scavenger, DAF-2. Thus, macrophage-induced nitrosylation/oxidation plays an important role in cell clearance, and hence in the ...

*  February 2006 - Volume 17 - Issue 2 : Anti-Cancer Drugs

Difference of cell cycle arrests induced by lidamycin in human breast cancer cells. Liu, Xia; He, Hongwei; Feng, Yun; More ... Relationships between DNA strand breakage and apoptotic progression upon treatment of HL-60 leukemia cells with tafluposide or ... Decrease in c-Myc activity enhances cancer cell sensitivity to vinblastine. Bressin, Céline; Bourgarel-Rey, Véronique; Carré, ... expression in human glioblastoma cells. Gagliano, Nicoletta; Moscheni, Claudia; Torri, Carlo; More ...

*  Molecules | Free Full-Text | In Vitro Antioxidant and Cytotoxic Activity of Some Synthetic Riparin-Derived Compounds | HTML

All the substances showed antioxidant activity and riparins C, D, E, F presented cell growth inhibition rates greater than 70 ... This study aimed to study the in vitro antioxidant activity and cytotoxicity on tumor cells lines of six synthetic substances ... Table 5 shows the cytotoxicity of riparins (IC50) upon three cancer cells lines: HL-60 (pro-myelocytic leukemia), NCIH-292 ( ... 100 µL of tumor cells were plated in 96-well plates (6 × 104 cells/cm2 for adherent cells or 9 × 104 cells/cm2 for leukemia). ...

*  Anti-intestinal alkaline phosphatase antibody (ab70975)

HL60 cell line lysate at 35 µg. Predicted band size : 57 kDa. Observed band size : 57 kDa. Additional bands at : 40 kDa,72 kDa ... Cell Biology. Epigenetics. Metabolism. Developmental Biology. By research area. Immunology. Microbiology. Neuroscience. Signal ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ... Anti-intestinal alkaline phosphatase antibody (ab70975) at 1/60 dilution + ...

*  Star Trek (2009) / Headscratchers - TV Tropes

Iowa has rolling hills. * Beware Small Reference Pools. Kirk doesn't traverse more than a handful of miles of his own part of ... Cells in the hair follicle generate keratin, and various other proteins, which become a part of the hair shaft. These proteins ... It's a TOS/60's setting quirk. Other Star Trek series have gender neutral and really boring jump suits. ... As for fuel... maybe it was converted to use Hydrogen fuel cells or some other clean fuel. There was an episode of Babylon 5 ...

*  HLA supertype variation across populations: new insights into the role of natural selection in the evolution of HLA-A and HLA-B...

Gilchuk P, Spencer CT, Conant SB, Hill T, Gray JJ, Niu X, Zheng M, Erickson JJ, Boyd KL, McAfee KJ, Oseroff C, Hadrup SR, ... functional and evolutionary interactions with T cells. Cold Spring Harb Symp Quant Biol 54(Pt 1):529-543CrossRefPubMedGoogle ... T cell epitopes restricted within common HLA supertypes for therapeutic HIV type 1 vaccines. AIDS Res Hum Retroviruses 28(11): ... Discovering naturally processed antigenic determinants that confer protective T cell immunity. J Clin Invest 123(5):1976-1987 ...

*  Human Metabolome Database: Showing metabocard for TG(16:1(9Z)/18:1(9Z)/20:4(5Z,8Z,11Z,14Z)) (HMDB0005441)

At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore ... May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the ... Fat cells can synthesize and store triglycerides. When the body requires fatty acids as an energy source, the hormone glucagon ... Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone ...

*  Tissue expression of COX19 - Staining in soft tissue 2 - The Human Protein Atlas

Cell cycle intensity correlation. Cell cycle spatial correlation. Cell cycle biologically. Custom data cell cycle dependant. - ... HL-60. HMC-1. HTCEpi. HUVEC TERT2. K-562. Karpas-707. LHCN-M2. MCF7. MOLT-4. NB-4. NTERA-2. PC-3. REH. RH-30. RPMI-8226. RPTEC ... RNA cell category. RNA cancer category. Tissue detectable (RNA). Cell line detectable (RNA). Cancer detectable (RNA). ... Cell line enriched. Group enriched. Cell line enhanced. Elevated. Expressed in all. Mixed. Not detected. ...

*  In Vitro Fertilization | Blake Medical Center | Bradenton, FL

Once fertilized, early cell division begins and embryos develop.. Transferring Procedure. A certain number of the embryos ( ... Oak Hill Hospital * Palms of Pasadena * RMC Bayonet Point * St. Petersburg General Hospital ... During that time, sperm are expected to fertilize 60%-80% of the eggs. ...

(1/3362) Epstein-barr virus regulates c-MYC, apoptosis, and tumorigenicity in Burkitt lymphoma.

Loss of the Epstein-Barr virus (EBV) genome from Akata Burkitt lymphoma (BL) cells is coincident with a loss of malignant phenotype, despite the fact that Akata and other EBV-positive BL cells express a restricted set of EBV gene products (type I latency) that are not known to overtly affect cell growth. Here we demonstrate that reestablishment of type I latency in EBV-negative Akata cells restores tumorigenicity and that tumorigenic potential correlates with an increased resistance to apoptosis under growth-limiting conditions. The antiapoptotic effect of EBV was associated with a higher level of Bcl-2 expression and an EBV-dependent decrease in steady-state levels of c-MYC protein. Although the EBV EBNA-1 protein is expressed in all EBV-associated tumors and is reported to have oncogenic potential, enforced expression of EBNA-1 alone in EBV-negative Akata cells failed to restore tumorigenicity or EBV-dependent down-regulation of c-MYC. These data provide direct evidence that EBV contributes to the tumorigenic potential of Burkitt lymphoma and suggest a novel model whereby a restricted latency program of EBV promotes B-cell survival, and thus virus persistence within an immune host, by selectively targeting the expression of c-MYC.  (+info)

(2/3362) Reactive oxygen intermediate-dependent NF-kappaB activation by interleukin-1beta requires 5-lipoxygenase or NADPH oxidase activity.

We previously reported that the role of reactive oxygen intermediates (ROIs) in NF-kappaB activation by proinflammatory cytokines was cell specific. However, the sources for ROIs in various cell types are yet to be determined and might include 5-lipoxygenase (5-LOX) and NADPH oxidase. 5-LOX and 5-LOX activating protein (FLAP) are coexpressed in lymphoid cells but not in monocytic or epithelial cells. Stimulation of lymphoid cells with interleukin-1beta (IL-1beta) led to ROI production and NF-kappaB activation, which could both be blocked by antioxidants or FLAP inhibitors, confirming that 5-LOX was the source of ROIs and was required for NF-kappaB activation in these cells. IL-1beta stimulation of epithelial cells did not generate any ROIs and NF-kappaB induction was not influenced by 5-LOX inhibitors. However, reintroduction of a functional 5-LOX system in these cells allowed ROI production and 5-LOX-dependent NF-kappaB activation. In monocytic cells, IL-1beta treatment led to a production of ROIs which is independent of the 5-LOX enzyme but requires the NADPH oxidase activity. This pathway involves the Rac1 and Cdc42 GTPases, two enzymes which are not required for NF-kappaB activation by IL-1beta in epithelial cells. In conclusion, three different cell-specific pathways lead to NF-kappaB activation by IL-1beta: a pathway dependent on ROI production by 5-LOX in lymphoid cells, an ROI- and 5-LOX-independent pathway in epithelial cells, and a pathway requiring ROI production by NADPH oxidase in monocytic cells.  (+info)

(3/3362) Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells.

The human granulocytic ehrlichiosis (HGE) agent resides and multiplies exclusively in cytoplasmic vacuoles of granulocytes. Double immunofluorescence labeling was used to characterize the nature of the HGE agent replicative inclusions and to compare them with inclusions containing the human monocytic ehrlichia, Ehrlichia chaffeensis, in HL-60 cells. Although both Ehrlichia spp. can coinfect HL-60 cells, they resided in separate inclusions. Inclusions of both Ehrlichia spp. were not labeled with either anti-lysosome-associated membrane protein 1 or anti-CD63. Accumulation of myeloperoxidase-positive granules were seen around HGE agent inclusions but not around E. chaffeensis inclusions. 3-(2, 4-Dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange were not localized to either inclusion type. Vacuolar-type H+-ATPase was not colocalized with HGE agent inclusions but was weakly colocalized with E. chaffeensis inclusions. E. chaffeensis inclusions were labeled with the transferrin receptor, early endosomal antigen 1, and rab5, but HGE agent inclusions were not. Some HGE agent and E. chaffeensis inclusions colocalized with major histocompatibility complex class I and II antigens. These two inclusions were not labeled for annexins I, II, IV, and VI; alpha-adaptin; clathrin heavy chain; or beta-coatomer protein. Vesicle-associated membrane protein 2 colocalized to both inclusions. The cation-independent mannose 6-phosphate receptor was not colocalized with either inclusion type. Endogenously synthesized sphingomyelin, from C6-NBD-ceramide, was not incorporated into either inclusion type. Brefeldin A did not affect the growth of either Ehrlichia sp. in HL-60 cells. These results suggest that the HGE agent resides in inclusions which are neither early nor late endosomes and does not fuse with lysosomes or Golgi-derived vesicles, while E. chaffeensis resides in an early endosomal compartment which accumulates the transferrin receptor.  (+info)

(4/3362) A photodynamic pathway to apoptosis and necrosis induced by dimethyl tetrahydroxyhelianthrone and hypericin in leukaemic cells: possible relevance to photodynamic therapy.

The mechanism of cell death induction by dimethyl tetrahydroxyhelianthrone (DTHe), a new second-generation photodynamic sensitizer, is analysed in human leukaemic cell lines in comparison with the structurally related hypericin. DTHe has a broad range of light spectrum absorption that enables effective utilization of polychromatic light. Photosensitization of HL-60 cells with low doses of DTHe (0.65 microM DTHe and 7.2 J cm(-2) light energy) induced rapid apoptosis of > or =90% of the cells. At doses > or =2 microM, dying cells assumed morphological necrosis with perinucleolar condensation of chromatin in HL-60 and K-562 cell lines. Although nuclear fragmentation that is characteristic to apoptosis was prevented, DNA digestion to oligonucleosomes proceeded unhindered. Such incomplete apoptosis was more prevalent with the related analogue hypericin throughout most doses of photosensitization. Despite hypericin being a stronger photosensitizer, DTHe exhibited advantageous phototoxic properties to tumour cells, initiating apoptosis at concentrations about threefold lower than hypericin. Photosensitization of the cells induced dissociation of the nuclear envelope, releasing lamins into the cytosol. DTHe also differed from hypericin in effects exerted on the nuclear lamina, causing release of an 86-kDa lamin protein into the cytosol that was unique to DTHe. Within the nucleus, nuclear envelope lamin B underwent covalent polymerization, which did not affect apoptotic nuclear fragmentation at low doses of DTHe. At higher doses, polymerization may have been extensive enough to prevent nuclear collapse. Hut-78, CD4+ cells were resistant to the photodynamically activated apoptotic pathway. Beyond the tolerated levels of photodynamic damage, these cells died exclusively via necrosis. Hut-78 cells overexpress Bcl-X(L) as well as a truncated Bcl-X(L)tr isoform that could contribute to the observed resistance to apoptosis.  (+info)

(5/3362) Heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor expression by acute myeloid leukemia cells.

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is an EGF family member expressed by numerous cell types that binds to EGF receptor 1 (HER-1) or 4 (HER-4) inducing mitogenic and/or chemotactic activities. Membrane-bound HB-EGF retains growth activity and adhesion capabilities and the unique property of being the receptor for diphtheria toxin (DT). The interest in studying HB-EGF in acute leukemia stems from these mitogenic, chemotactic, and receptor functions. We analyzed the expression of HB-EGF in L428, Raji, Jurkat, Karpas 299, L540, 2C8, HL-60, U937, THP-1, ML-3, and K562 cell lines and in primary blasts from 12 acute myeloid leukemia (AML) cases, by reverse-transcriptase polymerase chain reaction (RT-PCR) and Northern blot and by the evaluation of sensitivity to DT. The release of functional HB-EGF was assessed by evaluation of its proliferative effects on the HB-EGF-sensitive Balb/c 3T3 cell line. HB-EGF was expressed by all myeloid and T, but not B (L428, Raji), lymphoid cell lines tested, as well as by the majority (8 of 12) of ex vivo AML blasts. Cell lines (except for the K562 cell line) and AML blasts expressing HB-EGF mRNA underwent apoptotic death following exposure to DT, thus demonstrating the presence of the HB-EGF molecule on their membrane. Leukemic cells also released a fully functional HB-EGF molecule that was mitogenic for the Balb/c 3T3 cell line. Factors relevant to the biology of leukemic growth, such as tumor necrosis factor-alpha (TNF-alpha), 1alpha,25-(OH)2D3, and especially all-trans retinoic acid (ATRA), upregulated HB-EGF mRNA in HL-60 or ML-3 cells. Granulocyte-macrophage colony-stimulating factor (GM-CSF) induced HB-EGF mRNA and acquisition of sensitivity to DT in one previously HB-EGF-negative leukemia case. Moreover, the U937 and Karpas 299 cell lines expressed HER-4 mRNA. This work shows that HB-EGF is a growth factor produced by primary leukemic cells and regulated by ATRA, 1alpha, 25-(OH)2D3, and GM-CSF.  (+info)

(6/3362) Interaction of 5-lipoxygenase with cellular proteins.

5-Lipoxygenase (5LO) plays a pivotal role in cellular leukotriene synthesis. To identify proteins interacting with human 5LO, we used a two-hybrid approach to screen a human lung cDNA library. From a total of 1.5 x 10(7) yeast transformants, nine independent clones representing three different proteins were isolated and found to specifically interact with 5LO. Four 1.7- to 1.8-kb clones represented a 16-kDa protein named coactosin-like protein for its significant homology with coactosin, a protein found to be associated with actin in Dictyostelium discoideum. Coactosin-like protein thus may provide a link between 5LO and the cytoskeleton. Two other yeast clones of 1.5 kb encoded transforming growth factor (TGF) type beta receptor-I-associated protein 1 partial cDNA. TGF type beta receptor-I-associated protein 1 recently has been reported to associate with the activated form of the TGF beta receptor I and may be involved in the TGF beta-induced up-regulation of 5LO expression and activity observed in HL-60 and Mono Mac 6 cells. Finally, three identical 2.1-kb clones contained the partial cDNA of a human protein with high homology to a hypothetical helicase K12H4. 8 from Caenorhabditis elegans and consequently was named DeltaK12H4. 8 homologue. Analysis of the predicted amino acid sequence revealed the presence of a RNase III motif and a double-stranded RNA binding domain, indicative of a protein of nuclear origin. The identification of these 5LO-interacting proteins provides additional approaches to studies of the cellular functions of 5LO.  (+info)

(7/3362) Bcl-2 alters the balance between apoptosis and necrosis, but does not prevent cell death induced by oxidized low density lipoproteins.

Oxidized low density lipoproteins (oxLDL) participate in atherosclerosis plaque formation, rupture, and subsequent thrombosis. Because oxLDL are toxic to cultured cells and Bcl-2 protein prevents apoptosis, the present work aimed to study whether Bcl-2 may counterbalance the toxicity of oxLDL. Two experimental model systems were used in which Bcl-2 levels were modulated: 1) lymphocytes in which the (high) basal level of Bcl-2 was reduced by antisense oligonucleotides; 2) HL60 and HL60/B (transduced by Bcl-2) expressing low and high Bcl-2 levels, respectively. In cells expressing relatively high Bcl-2 levels (lymphocytes and HL60/B), oxLDL induced mainly primary necrosis. In cells expressing low Bcl-2 levels (antisense-treated lymphocytes, HL60 and ECV-304 endothelial cells), the rate of oxLDL-induced apoptosis was higher than that of primary necrosis. OxLDL evoked a sustained calcium rise, which is a common trigger to necrosis and apoptosis since both types of cell death were blocked by the calcium chelator EGTA. Conversely, a sustained calcium influx elicited by the calcium ionophore A23187 induced necrosis in cells expressing high Bcl-2 levels and apoptosis in cells expressing low Bcl-2 levels. This suggests that Bcl-2 acts downstream from the calcium peak and inhibits only the apoptotic pathway, not the necrosis pathway, thus explaining the apparent shift from oxLDL-induced apoptosis toward necrosis when Bcl-2 is overexpressed.  (+info)

(8/3362) Effects of novel RAR- and RXR-selective retinoids on myeloid leukemic proliferation and differentiation in vitro.

Retinoids such as all-trans-retinoic acid (ATRA) and 9-cis-retinoic acid (9-cis-RA) have an important role in many aspects of proliferation and differentiation of hematopoietic cells. They exert their effects by binding to retinoic acid receptors (RARs) and/or retinoid X receptors (RXRs). We studied the effects of novel retinoids on proliferation and differentiation of HL-60 and NB4 myeloid leukemic cells, as well as acute promyelocytic leukemia (APL) cells from patients. RXR-selective SR11345 (Retinoid C) had little ability to inhibit the clonal growth and to induce the differentiation of either HL-60 or NB4 cells. However, SR11276 (Retinoid E), which activated both the RAR and RXR classes, and SR11278 (Retinoid D), which activated the RAR subtypes alpha, beta, and gamma, could inhibit clonal growth of both cell types, as well as leukemic cells from APL patients. The combination of ATRA and either SR11276 or SR11278 additively inhibited APL cell proliferation. SR11302 (Retinoid A), with reported anti-AP-1 activity and no activation of RARs and RXR and SR11363 (Retinoid B), which selectively activated RARbeta and gamma, were inactive. The clonal proliferation of both HL-60 and NB4 cells that were pulse-exposed to 10(-9) mol/L ATRA, SR11276, SR11278, or SR11345 for 3 days, washed, and plated in methylcellulose culture were inhibited by 0%, 51%, 21%, and 1% for HL-60 cells and 43%, 41%, 35%, and 1% for NB4, respectively, compared with nontreated control cells. When the HL-60 cells were pulse-exposed to 10(-9) mol/L of either SR11278 or SR11276, plus 10(-9) mol/L ATRA for 3 days, colony numbers were reduced by 46% and 64%, respectively. Induction of leukemic cell differentiation as determined by the nitroblue tetrazolium (NBT) assay showed that the combination of 10(-7) mol/L of either SR11278 or SR11276 with 10(-7) mol/L ATRA had additive effects on HL-60 cells, NB4 cells, and fresh APL cells. Induction of CD11b expression on both HL-60 and NB4 cells occurs during their differentiation. Expression of this antigen was synergistically augmented by the combination of either 10(-7) to 10(-8) mol/L SR11278 or 10(-7) to 10(-9) mol/L SR11276 with 10(-9) mol/L ATRA compared with either analog alone in HL-60 cells. Expression of the novel myeloid specific transcription factor C/EBPepsilon was increased by SR11278 and SR11276 in both the HL-60 and NB4 cell lines. We conclude that retinoids or combination of retinoids with specificities for both RAR and RXR may markedly enhance the ability of ATRA to inhibit clonal growth and induce differentiation of HL-60 and NB4 leukemic cells. This occurs in the absence of continuous contact with retinoids.  (+info)



inhibition

  • The idea that inhibition of membrane sialylation by chemotherapeutic drugs may alter cell surface properties of tumor cells and contribute to the antineoplastic effect should be further explored. (aacrjournals.org)
  • Examples of appropriate concentrations for some common inducers of HL-60 differentiation are shown in Table I. For new chemical inducers, a series of concentrations of the inducer should be tested, and conditions that impart an inhibition of cell multiplication and the appearance of one or more myelomonocytic differentiation markers should be tested further. (biocyclopedia.com)
  • Because activated macrophages produce reactive oxygen and nitrogen species, we hypothesized that macrophages can directly participate in apoptotic cell clearance by S-nitrosylation/oxidation and inhibition of APLT causing PS externalization. (cdc.gov)
  • All the substances showed antioxidant activity and riparins C, D, E, F presented cell growth inhibition rates greater than 70%, suggesting that these molecules have antitumor properties. (mdpi.com)
  • That is, it was inhibitory of glycolipid biosynthesis at relatively low concentrations (about 50 μM) compared to the mM level of concentration in cell culture systems for α-glucosidase I inhibition [Karlsson et al. (google.com)
  • These results suggest that the asparagus extract may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells. (sigmaaldrich.com)

Apoptotic

  • In the present study, we further investigated the apoptotic mechanisms of ATO toxicity using the HL-60 cell line as a test model. (biomedcentral.com)
  • Macrophage recognition of apoptotic cells depends on externalization of phosphatidylserine (PS), which is normally maintained within the cytosolic leaflet of the plasma membrane by aminophospholipid translocase (APLT). (cdc.gov)
  • Acceleration of apoptotic cell death after the cleavage of Bcl-XL protein by caspase-3-like proteases. (nii.ac.jp)

promyelocytic leukemia cells

  • A recent study from our laboratory has indicated that ATO induces transcription of specific genes that modulate mitogen response, cell cycle progression, programmed cell death, and cellular function in cultured HL-60 promyelocytic leukemia cells. (biomedcentral.com)
  • Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. (atcc.org)

lysate

myeloid cell

  • The inhibitory effect of these compounds on the biosynthesis of glycolipids is illustrated herein in myeloid cell lines (e.g. (google.com)
  • Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. (atcc.org)

induces apoptosis

  • Collectively, our results showed that EG induces apoptosis in HL-60 via mitochondrial-mediated pathways. (mdpi.com)

leukemia cell line

  • In this study, we investigated ethyl gallate (EG) for possible proapoptotic effects in the human promyelocytic leukemia cell line, HL-60. (mdpi.com)
  • Arachidonic acid metabolism by nuclei of a retinoic acid--or vitamin D3-differentiated human leukemia cell line HL-60. (biomedsearch.com)
  • The human myeloid HL-60 leukemia cell line has enjoyed widespread use in studies of the molecular mechanisms involved in the control of cell growth, differentiation, and apoptosis. (biocyclopedia.com)

human

  • Human leukemia HL60 cells respond to the presence of 1,25-dihydroxyvitamin D3 (1,25D3) by expressing a number of markers of the monocyte/macrophage phenotype and become arrested predominantly in the G1 phase of the cell cycle. (nih.gov)
  • Arachidonic acid (AA) metabolism in nuclei of human pro-myelocytic leukemia (HL-60) cells was investigated during retinoic acid (RA)-induced granulocytic differentiation and 1 alpha, 25 dihydroxy-vitamin D3-induced monocytic differentiation. (biomedsearch.com)
  • In a recently published study we demonstrated that ATO pharmacology as an anti-cancer drug is associated with its cytotoxic and genotoxic effects in human leukemia cells. (biomedcentral.com)
  • Human gp91 phox is a highly glycosylated protein and appears on Western blots as a broad smear from 120 kDa to 60 kDa, average approx. (biochemj.org)
  • The Fer tyrosine kinase cooperates with interleukin-6 to activate signal transducer and activator of transcription 3 and promote human prostate cancer cell growth. (abcam.com)
  • A method of treating psoriasis in a patient which comprises administering to said patient an effective amount of a vitamin D compound which is capable of stimulating the differentiation of cultured tumor cells or normal rodent or human fibroblasts or keratinocytes in vitro. (google.com)
  • These are well-known, widely distributed and readily available human cell lines. (google.com)
  • The asparagus crude saponins (ACS) inhibited the growth of human leukemia HL-60 cells in culture and macromolecular synthesis in a dose and time dependent manner. (sigmaaldrich.com)
  • These two compounds have been shown to inhibit the growth of human leukemia HL-60 cells in culture and macromolecular synthesis in a dose-dependent manner. (sigmaaldrich.com)
  • Nyasol (5) and 11 from asparagus exhibited remarkable in vitro cytotoxic activity against cultured HO-8910 (human ovarian carcinoma) and Bel-7402 (human hepatoma) cells with IC 50 vales of 30.6 and 29.4 microM, 5.2 and 5.2 microM, respectively. (sigmaaldrich.com)
  • Oncogenic Ras triggers cell suicide through the activation of a caspase-independent cell death program in human cancer cell. (nii.ac.jp)
  • While the nucleosome positioning at RNA polymerase II (pol II) promoters has been extensively studied, less is known about the chromatin structure at pol III promoters in human cells. (nature.com)
  • Listeria monocytogenes Inhibits Serotonin Transporter in Human Intestinal Caco-2 Cells. (abcam.com)

viability

  • We examined cell viability, morphological changes, DNA content and fragmentation, and expression of apoptosis-related proteins for up to 48 h after EG treatment. (mdpi.com)
  • The results showed that EG induced morphological changes and DNA fragmentation and reduced HL-60 cell viability in a dose-dependent and time-dependent manner. (mdpi.com)
  • The MTT assay was used to investigate cell viability after ATX treatment, and western blot analysis was used to observe Sp1 (activated transcription factor 1) and NR1 (NMDA receptor subunit 1) protein expression, real-time PCR was used to monitor Sp1 and NR1 mRNA, and cell immunofluorescence was used to determine the location of Sp1 and NR1 protein and the nuclear translocation of Sp1. (mdpi.com)

protein

  • Western blotting analysis indicated that EG-mediated HL-60 apoptosis mainly occurred through the mitochondrial pathway, as shown by the release of cytochrome c , apoptosis-inducing factor (AIF), and endonuclease G (Endo G), as well as the upregulation of Bcl-2-associated X protein (Bax). (mdpi.com)
  • The ACS at 6 and 50micrograms/mL inhibited the synthesis of DNA, RNA and protein in HL-60 cells by 41, 5, and 4, respectively, or by 84, 68 and 59%, respectively. (sigmaaldrich.com)
  • Unperturbed cells express very little TF PCA despite the fact that TF, an integral membrane protein, is on the cell surface where it can bind zymogen factor VII (FVII) as well as activated FVII (FVIIa). (ahajournals.org)
  • At a protein concentration of 44 µg/mL, the 50 kDa fraction induced a mortality of 90% for PC3, 89% for A549, 85% for HCT15 and of 81% for BT549 cell lines. (mdpi.com)
  • At the low protein concentration of only 11 µg/mL the 50 kDa fraction still entails a cell mortality of 76% for A549 and 87% for PC3 cell lines. (mdpi.com)

phagocytosis

  • SNCEE but not GSNO stimulated phagocytosis of HL-60 cells. (cdc.gov)
  • Moreover, phagocytosis of target cells by lipopolysaccharide-stimulated macrophages was significantly suppressed by an NO. scavenger, DAF-2. (cdc.gov)

1997

antibody

  • Monoclonal antibody (mAb) 7D5 [ 10 , 11 ] and polyclonal anti-L 123 (S 151 YLNFARKRIKNPEGGLYLAVTL 173 ) antibody [ 12 ] have both been reported to bind to intact neutrophils, demonstrating that their epitopes are exposed to the exterior of the cell. (biochemj.org)

recombinant

  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)

monocytic

  • Uncoupling of cell cycle arrest from the expression of monocytic differentiation markers in HL60 cell variants. (nih.gov)
  • These results show that the initial steps in the 1,25D3 signaling pathway are intact in B series resistant cells and lead to the appearance of early markers of monocytic differentiation. (nih.gov)

toxicity

  • a pre-activated photoactive compound affixed to said conveyor, said pre-activated photoactive compound being characterized as having a preferential toxicity toward undesirable cells or pathogenic biological contaminants infecting an animal body or tissue, and as having therapeutic activities for at least long enough to destroy said undesirable cells or pathogenic biological contaminants. (google.com)

lymphoid

  • Expressed in a variety of lymphoid cell lines. (abcam.com)
  • HL-60 and K-562) as well as in lymphoid cell lines (e.g. (google.com)

apoptosis

  • Apoptosis is a central mechanism to eliminate unwanted cells that may accumulate during physiological processes and pathologic conditions such as cancer and autoimmune disease [ 6 ]. (mdpi.com)
  • Mitochondria are involved in a variety of cellular processes and functions, such as cell differentiation, growth, survival, and apoptosis [ 9 , 10 ]. (mdpi.com)
  • 0.05) was recorded with regard to caspase 3 activity in HL60 cells undergoing late apoptosis. (biomedcentral.com)
  • Furthermore, we found that the asparagus extract inhibited the TNF-alpha-induced apoptosis of Hep G2 cells. (sigmaaldrich.com)
  • Resistance to Fas-Mediated apoptosis: activation of caspase-3 is regulated by cell cycle regulator p21 WAF and IAP gene family ILP. (nii.ac.jp)
  • Involvement of Bcl-2 cleavage in the acceleration of VP-16-induced U937 cell apoptosis. (nii.ac.jp)
  • Caspase-mediated cleavage of p21 Waf 1/Cip 1 converts cancer cells from growth arrest to undergoing apoptosis. (nii.ac.jp)

progression

  • However, the progression to subsequent events which comprise terminal differentiation and cell cycle arrest is halted during the adaptation to the presence of 1,25D3 in these cells. (nih.gov)

line

  • HL60 cell line lysates. (abcam.com)
  • This cell line is a suitable transfection host. (atcc.org)
  • HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. (atcc.org)
  • The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. (atcc.org)

concentrations

  • We now describe another series (B) of such variants, which differ from A series cells grown in similar concentrations of 1,25D3 in that they express the CD14 antigen and nonspecific esterase, characteristic of the monocyte, while continuing to proliferate and they develop hypotetraploid DNA (4C) content at higher concentrations of ambient 1,25D3 than the A series cells. (nih.gov)
  • ACS concentrations greater than 200micrograms/mL were cytocidal to HL-60 cells. (sigmaaldrich.com)

sensitivity

  • The most effective concentration for the induction of differentiation depends on the potency of individual lots of the inducer and the endogenous sensitivity of the HL-60 cells being used. (biocyclopedia.com)

activity

  • This study aimed to study the in vitro antioxidant activity and cytotoxicity on tumor cells lines of six synthetic substances derived from riparins. (mdpi.com)
  • 1. The method of inhibiting glycosyltransferase activity for biosynthesis of glycosylceramide-based bacterial cell receptors in a patient in need of antibacterial treatment comprising administering to said patient an effective amount for inhibiting said glycosyltransferase activity of an N-alkyl derivative of 1,5-dideoxy-1,5-imino-D-glucitol in which said alkyl contains from 2-8 carbon atoms. (google.com)
  • The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. (atcc.org)
  • Tissue factor (TF) encryption is the post-translational suppression of TF procoagulant activity (PCA) on the cell surface. (ahajournals.org)
  • The 50 kDa fraction, enriched in peptides, presented anti-proliferative activity with all cell lines and results suggest a bioactive molecule synergy within the fraction. (mdpi.com)

tissue

  • The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. (qiagen.com)
  • Genomic DNA can be purified from cells, tissue, and blood. (qiagen.com)

endothelial cells

  • TF antigen was detected on cells surrounding blood vessels, but it was not visible on either endothelial cells or cells in the bloodstream. (ahajournals.org)

exposure

  • Flow cytometry data showed a strong dose-response relationship between ATO exposure and Annexin-V positive HL-60 cells. (biomedcentral.com)
  • Here we report that exposure of target HL-60 cells to nitrosative stress inhibited APLT, induced PS externalization, and enhanced recognition and elimination of "nitrosatively" modified cells by RAW 264.7 macrophages. (cdc.gov)
  • A large calcium influx triggers both the exposure of phosphatidylserine and the expression of TF PCA on cell surfaces. (ahajournals.org)

vitro cell

  • Methods: Mature, differentiated PC12 cells treated with MPP + were used as an in vitro cell model. (mdpi.com)

pathway

  • Caspase-independent cell death with necrotic morphology: New insight into the signaling pathway for programmed cell death. (nii.ac.jp)

growth

  • However, B series cells resembled the A series cells in exhibiting faster growth rates than the parental HL60 cells and showed high levels of vitamin D receptor and retinoid receptor X proteins. (nih.gov)

processes

  • Probably performs an important function, perhaps in regulatory processes such as cell cycle control. (abcam.com)

tissues

  • The BioSprint 96 DNA Blood Kit provides all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots, and buccal swabs using magnetic particle technology on the BioSprint 96. (qiagen.com)
  • Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)

differentiation markers

  • We have recently reported a series (A) of 1,25D3-resistant variants of HL60 cells which proliferate in the presence of 1,25D3 and do not express differentiation markers (Exp. (nih.gov)

membrane

  • Because alterations in cell membrane sialoglycoconjugates can affect the behavior of neoplastic cells, we investigated the effects of in vitro treatment with antimetabolites used in cancer therapy on the expression of membrane sialic acid in cultured HL-60 leukemic cells. (aacrjournals.org)
  • Reappearance of membrane sialic acid during drug treatment was followed ( a ) by measuring changes in radioactive surface labeling of viable cells with sodium metaperiodate-sodium[ 3 H]-borohydride, ( b ) by measuring the decline in accessible surface galactosyl receptor sites which occurred coincident with membrane sialic acid replacement, and ( c ) by measuring the incorporation of [ 3 H]glucosamine into membrane-associated neuraminidase-labile sialic acid. (aacrjournals.org)
  • Doxorubicin, at levels that caused marked arrest of cell proliferation, had no effect on sialic acid synthesis or expression on the membrane surface. (aacrjournals.org)
  • The significance of the acquisition of cyclooxygenase and TX synthetase by the nucleus is unclear, but there may be a specific relationship between the specific PGs formed by the nuclear membrane and nuclear events during HL-60 cell differentiation. (biomedsearch.com)
  • Cell membrane. (abcam.com)
  • In my own work I have a strong preference for Ca 2+ ionophores because they rapidly induce the maximum expression of cell surface TF PCA without destroying plasma membrane integrity. (ahajournals.org)
  • In the plasma membrane of quiescent cells phosphatidylserine (PS) is sequestered on the inner leaflet of the bilayer. (ahajournals.org)

synthesis

  • This invention relates to a novel method of inhibiting glycolipid synthesis and, more particular, to the use of N-alkyl derivatives of 1,5-dideoxy-1,5-imino-D-glucitol for inhibiting glycolipid biosynthesis in cells capable of producing glycolipids, in which said alkyl groups contain from about 2-8 carbon atoms. (google.com)

viable

  • If cells are to be used for viable immunostaining, inactivate serum complement by heat inactivation (65°C for 15 min followed by slow cooling to room temperature). (biocyclopedia.com)
  • Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 5 viable cells/mL. (atcc.org)
  • Maintain cell density between 1 x 10 5 and 1 x 10 6 viable cells/mL. (atcc.org)

neutrophils

  • Immunocytochemistry and flow-cytometry analysis show a strong binding of the anti-N-terminal antibodies to differentiated HL60 cells and neutrophils respectively, after permeabilization only. (biochemj.org)

antibodies

  • 30% off on 400+ Stem Cells Antibodies. (abgent.com)

respectively

  • Using S-nitroso-L-cysteine-ethyl ester (SNCEE) and S-nitrosoglutathione (GSNO) that cause intracellular and extracellular trans-nitrosylation of proteins, respectively, we found that SNCEE (but not GSNO) caused significant S-nitrosylation/oxidation of thiols in HL-60 cells. (cdc.gov)

culture

  • TF encryption was first observed in cell culture studies. (ahajournals.org)

nuclei

  • The whole control HL-60 cells and their nuclei hardly converted [1-14C]-AA to any metabolites comigrating with authentic prostaglandins (PGs). (biomedsearch.com)
  • On the other hand, RA-treated HL-60 cells acquired the ability to convert [1-14C]-AA to PGE2 predominantly and thromboxane B2 (TXB2) to a small degree, whereas the nuclei of the differentiated cells acquired the ability to convert predominantly to TXB2. (biomedsearch.com)
  • In contrast, 1 alpha, 25-dihydroxy-vitamin D3-treated HL-60 cells acquired the ability to convert [1-14C]-AA to PGE2, PGF2 alpha, TXB2 and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), whereas the nuclei of the differentiated cells acquired the ability to convert to PGF2 alpha, TXB2 and HHT. (biomedsearch.com)

treatment

  • In accordance with the present invention, a method is provided for inhibiting the biosynthesis of glycolipids in cells capable of producing glycolipids which comprises treatment of said cells with a glycolipid inhibitory effective amount of an N-alkyl derivative of 1,5-dideoxy-1,5-imino-D-glucitol (DNJ) in which said alkyl contains from 2-8 carbon atoms and preferably from 4-6 carbon atoms. (google.com)

Thus

  • Thus, the availability of these variant cells should provide a system for studying the link between differentiation and cell cycle arrest. (nih.gov)
  • Thus, macrophage-induced nitrosylation/oxidation plays an important role in cell clearance, and hence in the resolution of inflammation. (cdc.gov)

lines

  • Fractions were tested on four immortalized cancerous cell lines: A549, BT549, HCT15 and PC3. (mdpi.com)
  • Highly expressed in promyelocytic HL-60 cells and in B-cell lines. (abcam.com)

assay

  • These results were confirmed by data of DNA laddering assay showing a clear evidence of nucleosomal DNA fragmentation in ATO-treated cells. (biomedcentral.com)
  • In a prothrombin time assay, this concentration of pure TF, optimally reconstituted into phospholipids vesicles, clots plasma in &60 seconds (R. Bach, unpublished, 2005). (ahajournals.org)

expression

  • It may be the primary mechanism controlling the expression of TF PCA by cells in blood. (ahajournals.org)

arrest

  • Differentiation generally leads to cell cycle arrest. (nih.gov)

studies

  • In these studies, cells were incubated with Vibrio cholerae neuraminidase to remove surface sialic acid. (aacrjournals.org)

effects

  • 14,17-27 These procedures vary significantly with respect to the level of TF PCA achieved as well as secondary effects on cell structure. (ahajournals.org)