Genetic Techniques: Chromosomal, biochemical, intracellular, and other methods used in the study of genetics.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genes, Bacterial: The functional hereditary units of BACTERIA.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

*  genetic techniques - Everything2.com
laboratory techniques. Genetic Sequence Comparisons. Microinjection. Using the closest available aerosol product as bug spray. ... and other scientists are generally interested in using one of three organisms with genetic techniques: yeast, transgenic mice, ... An example of this might be the study of a genetic disease like Lesch Nyhan's disease and the recognition that only men have ... or removal of a part of the genetic material the codes for a protein). For example, transgenic mice that have had their Myosin ...
  https://everything2.com/title/genetic+techniques
*  Genetic techniques have role in future of dental care
... 06.03.2014. A visit to the dentist could one day require a detailed look ... "Our genetic code, or DNA, is like an orchestra - it contains all of the elements we need to function - but the epigenetic code ... We can't change the underlying genetic code, but we may be able to change when genes are switched on and off," he says. ... Professor Hughes is part of a team of researchers at the University of Adelaide that has been studying the underlying genetic ...
  http://www.innovations-report.com/html/reports/life-sciences/genetic-techniques-have-role-in-future-of-dental-care.html
*  IDEALS @ Illinois: Sequential Circuit Test Generation Using Genetic Techniques
Sequential Circuit Test Generation Using Genetic Techniques. Welcome to the IDEALS Repository. ...
  https://www.ideals.illinois.edu/handle/2142/81208
*  The new age reproductive genetic techniques to drastically increase IVF success rate
... 01 November 2017 , Views ... The new age reproductive genetic techniques would drastically increase IVF success rates. ... Pre implantation Genetic Screening (PGS) is highly recommended for these couples. PGS along with MitoScore enables selection of ... This artificial or assisted technique of bestowing parenthood has brought a smile on millions of faces. The couples who fail to ...
  https://www.biospectrumindia.com/views/81/9758/the-new-age-reproductive-genetic-techniques-to-drastically-increase-ivf-success-rate.html
*  FishPopTrace: a new genetic technique for fisheries monitoring and the identification of IUU - DTU Orbit
FishPopTrace: a new genetic technique for fisheries monitoring and the identification of IUU. Publication: Research › ... However, many previous genetic studies have observed weak genetic structure in marine fish and, combined with a pelagic larval ... Low genetic and phenotypic divergence in a contact zone between freshwater and marine sticklebacks: gene flow constrains ... However, in most cases our understanding of these spatial patterns of natural variation at a genetic level is limited. For ...
  http://orbit.dtu.dk/en/publications/fishpoptrace-a-new-genetic-technique-for-fisheries-monitoring-and-the-identification-of-iuu
*  Powerful Genetic Engineering Technique Could Modify Entire Wild Populations
By combining two techniques-CRISPR and gene drives-scientists are proposing a system that could change nearly any sexually ... By combining two techniques-CRISPR and gene drives-scientists are proposing a system that could change nearly any sexually ... Scientists Cautiously OK the Next Genetic Revolution. Posted by Kelsey Houston-Edwards and Tim De Chant on Wed, 08 Jun 2016. ... "I don't care if it's a weed or a blight, people still are going to say this is way too massive a genetic engineering project," ...
  http://www.pbs.org/wgbh/nova/next/evolution/crispr-gene-drives/?utm_source=facebook&utm_medium=pbsofficial&utm_campaign=nova_next
*  Genetic Techniques - The Power of The Gene
... but since no actual tissues are sampled the technique cannot include genetic testing. ... Genetic fingerprinting is also employed in criminal cases to prove that the DNA found at a crime scene is statistically likely ... This non-invasive technique has been used to diagnose Down Syndrome.. The practice of prenatal diagnosis has recently come ... This technique could be used to analyse very large families to determine which individuals inherited a disease in combination ...
  http://powerofthegene.com/genetic-techniques/
*  Genetic engineering techniques - Wikipedia
... another method to deliver genetic modifications to plants List of genetic engineering software: software to code the genetic ... Corinne A. Michels (2002). "7". Genetic Techniques for Biological Research: A Case Study Approach. John Wiley & Sons. pp. 85-88 ... "The process of genetic modification". Nicholl, Desmond S. T. (2008-06-23). An Introduction to Genetic Engineering (third ed.). ... Knowledge of how the gene functions and interacts with other genetic elements is important. Genetic screens can be carried out ...
  https://en.wikipedia.org/wiki/Genetic_engineering_techniques
*  Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System | SpringerLink
... and genetic fluorescent labeling have become standard techniques to study the anatomy, function, and development of the ... Immunofluorescence (IF ) and genetic fluorescent labeling have become standard techniques to study the anatomy, function, and ... Selcho M., Wegener C. (2015) Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System. ... Kao C-F, Lee T (2012) In vivo single cell labeling techniques. Neuromethods 69:91-124CrossRefGoogle Scholar ...
  https://link.springer.com/protocol/10.1007/978-1-4939-2313-7_2
*  Innovative genetic and cellular techniques help identify multiple disease targets - BioPortfolio.com
Innovative genetic, cellular techniques help identify multiple disease targets *Scientists discover genetic markers for severe ... Innovative genetic and cellular techniques help identify multiple disease targets. 19:00 EST 11 Nov 2017 , AAAS ... More From BioPortfolio on "Innovative genetic and cellular techniques help identify multiple disease targets". *Related ... Original Article: Innovative genetic and cellular techniques help identify multiple disease targets ...
  https://www.bioportfolio.com/news/article/3414969/Innovative-genetic-and-cellular-techniques-help-identify-multiple-disease-targets.html
*  Controversial Technique Could Prevent Genetic Disease by Making 3-Parent Babies - 80beats : 80beats
Controversial Technique Could Prevent Genetic Disease by Making 3-Parent Babies. By Andrew Moseman , April 14, 2010 3:36 pm ... This technique was pioneered in monkeys last summer, but researchers have now done a proof-of-principle study with human cells. ... This technique has proven effective for many and there is evidence to prove it. Acupuncture treatment is more effective if the ... The good news: By combining the DNA of parents with genetic material from a third person, scientists might have developed a way ...
  http://blogs.discovermagazine.com/80beats/2010/04/14/controversial-technique-could-prevent-genetic-disease-by-making-3-parent-babies/
*  New technique will accelerate genetic characterization of phot... ( Stanford CA Photosynthesis provides fix...)
P,New,technique,will,accelerate,genetic,characterization,of,photosynthesis,biological,biology news articles,biology news today, ... New technique sheds light on human neural networks. 5. New technique for identifying gene-enhancers. 6. Heat-based technique ... New technique will accelerate genetic characterization of photosynthesis. Stanford CA Photosynthesis provides fixed carbon and ... New technique makes biogasoline from plant waste. 9. Facelift complications eased with help of new 3-D imaging technique. 10. ...
  http://www.bio-medicine.org/biology-news-1/New-technique-will-accelerate-genetic-characterization-of-photosynthesis-35173-1/
*  Development of Innovative Research Technique in Genetic Epidemiology of Malaria and Other Parasitic Diseases in Lao PDR for...
Development of Innovative Research Technique in Genetic Epidemiology of Malaria and Other Parasitic Diseases in Lao PDR for ... Molecular and genetic monitoring of parasitic diseases that threaten the people of Laos. The emergence and spread of drug- ... This project involves working with young Laotian researchers on molecular genetic epidemiology for these three significant ... parasitic diseases, with the aim of developing simple, rapid and precise diagnostic methods using LAMP techniques. The ...
  http://www.jst.go.jp/global/english/kadai/h2508_laos.html
*  A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT | Genetics
A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT. Reed C. Rollins ... A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT. Reed C. Rollins ... A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT. Reed C. Rollins ... A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT ...
  http://www.genetics.org/content/36/5/435
*  Adaptive PCR, a New Powerful Technique to Speed Up Genetic Analysis | Medgadget
Adaptive PCR, a New Powerful Technique to Speed Up Genetic Analysis. January 17th, 2017 Editors Genetics ... or amplifying DNA so there's enough of it to perform genetic analysis. The technique is called adaptive PCR and it relies on ... 19aprallday20allday4th World Congress on Human Genetics and Genetic Diseases, 2018Dubai, United Arab Emirates ... Having a way of continuously monitoring and guiding the process can lead to faster and cheaper results from genetic analysis ...
  https://www.medgadget.com/2017/01/adaptive-pcr-new-powerful-technique-speed-genetic-analysis.html
*  Life scientists use novel technique to produce genetic map for African... ( UCLA life scientists and colleagues ...)
... technique,to,produce,genetic,map,for,African,Americans,biological,biology news articles,biology news today,latest biology news, ... The new map will help disease geneticists working to map genetic disea...The study was published July 20 in the online version ... Life scientists use novel technique to produce genetic map for African Americans ... A genetic map reveals the precise locations across the genome where DNA from a person's father and mother have been stitched ...
  http://www.bio-medicine.org/biology-news-1/Life-scientists-use-novel-technique-to-produce-genetic-map-for-African-Americans-20470-1/
*  Complimenting CRISPR: Improving Genetic Delivery Techniques with Nanoparticles - Sciencers
Perfecting genetic editing could lead to clinic incorporation of genetic editing techniques, but is currently limited by the ... Complimenting CRISPR: Improving Genetic Delivery Techniques with Nanoparticles. Christina Cai and Erin Parlow ... New understanding of the CRISPR Cas-9 immunity system has led to the discovery of a more sophisticated technique for genetic ... This will give humans the ability to consistently make changes to organisms with techniques that operate in vivo. Genetic ...
  http://sciencers.org/index.php/blog/complimenting-crispr-improving-genetic-delivery-techniques-with-nanoparticles/
*  Genetic Nomenclature and Quantitative Techniques for Modern Genome Epidemiologists, Clinicians, Educators and other Behavioral...
This chapter explored the relevance of understanding genetic nomenclature in the age of genomic science. There are several ... Genetic Nomenclature and Quantitative Techniques for Modern Genome Epidemiologists, Clinicians, Educators and other Behavioral ... "Genetic Nomenclature and Quantitative Techniques for Modern Genome Epidemiologists, Clinicians, Educators and other Behavioral ... "Genetic Nomenclature and Quantitative Techniques for Modern Genome Epidemiologists, Clinicians, Educators and other Behavioral ...
  https://www.igi-global.com/chapter/genetic-nomenclature-and-quantitative-techniques-for-modern-genome-epidemiologists-clinicians-educators-and-other-behavioral-scientists/148504
*  Biology Study Guide: Genetic Engineering Techniques (13-1,2) - TechyLib
"το κείμενο με τίτλο Biology Study Guide: Genetic Engineering Techniques (13-1,2) σχετίζετε με Βιοτεχνολογία ... What does the technique of. gel. electrophoresis. allow molecular. biologists. and genetic engineers to do?. [1]. 6.. Why do ... Biology Study Guide: Genetic Engineering Techniques. (13.. 1,2). 1.. What is. selective breeding. ? [1]. 2.. Explain. how ... of genetic e. ngineers because they allow scientists to cut. the sugar. -. phosphate "backbones" of DNA molecules precisely. ...
  https://www.techylib.com/el/view/weedheightswaist/biology_study_guide_genetic_engineering_techniques_13-12
*  Change Gene Expression and Increase Glutathione with Relaxation Techniques | Genetic Genie
Change Gene Expression and Increase Glutathione with Relaxation Techniques. Managing stress with relaxation techniques such as ... Genetic Genie is powered by a little bit of sweat and your DNA.. This website is not promoted or endorsed by 23andMe, Inc.. ... Relaxation techniques change your gene expression. Dr. Herbert Benson did a study comparing 16 people who have regularly evoked ... Using relaxation techniques to boost glutathione. It's also important to note that people that are chronically ill often have ...
  http://geneticgenie.org/blog/2013/02/14/change-gene-expression-and-increase-glutathione-with-relaxation/
*  Penguinology: Ancient penguin DNA raises doubts about accuracy of genetic dating techniques
The findings, researchers say, are primarily a challenge to the techniques used to determine the age of a sample by genetic ... Ancient penguin DNA raises doubts about accuracy of genetic dating techniques. by on November 10, 2009 ... "For the genetic analysis to be accurate, however, you must have the right molecular clock rate," Denver said. "We now think ... The findings raise doubts about the accuracy of many evolutionary rates based on conventional types of genetic analysis.. "Some ...
  http://penguinology.blogspot.com/2010/07/ancient-penguin-dna-raises-doubts-about.html
*  New DNA cleavage technique could lead to more versatile genetic engineering
DNA fragments into certain genetic sequence locations. This process allows scientists ... ... Genetic engineering of plants, animals and microorganisms such as bacteria typically involves the use of restriction enzymes to ... New DNA cleavage technique could lead to more versatile genetic engineering. May 9, 2014, RIKEN ... Fast new, one-step genetic engineering technology. May 22, 2013 A new, streamlined approach to genetic engineering drastically ...
  https://phys.org/news/2014-05-dna-cleavage-technique-versatile-genetic.html
*  Lateralized Brain Functions | SpringerLink
This volume explores both simple and sophisticated techniques used in the study of different types of lateralization of brain ... genetic techniques; and development of lateralization. Part I addresses measuring lateralization by scoring behavior induced by ... Part III looks at imaging techniques, electroencephalographic techniques, and transcranial stimulation to reveal lateralization ... Part IV describes techniques used to study the role of genes in the development and establishment of brain asymmetry in humans ...
  https://link.springer.com/book/10.1007%2F978-1-4939-6725-4
*  10.3 Genetic and molecular techniques - Techniques in neuroscience | Coursera
Let's learn some prevalent techniques in neuroscience. Learn online and earn valuable credentials from top universities like ... 10.3 Genetic and molecular techniques. Чтобы просмотреть это видео, включите JavaScript и используйте веб-браузер, который ... So one need to develop this complicated genetic techniques to perturb,. both spatially and temporary, the gene's function to ... One can also use different techniques to control the spatial specificity.. Again, one can take advantage of different part of ...
  https://ru.coursera.org/lecture/advanced-neurobiology1/10-3-genetic-and-molecular-techniques-hQCpw
*  10.3 Genetic and molecular techniques - Techniques in neuroscience | Coursera
Let's learn some prevalent techniques in neuroscience. 2000+ courses from schools like Stanford and Yale - no application ... 10.3 Genetic and molecular techniques. To view this video please enable JavaScript, and consider upgrading to a web browser ... So one need to develop this complicated genetic techniques to perturb,. both spatially and temporary, the gene's function to ... One can also use different techniques to control the spatial specificity.. Again, one can take advantage of different part of ...
  https://es.coursera.org/learn/advanced-neurobiology1/lecture/hQCpw/10-3-genetic-and-molecular-techniques

(1/2595) Induction of AT-specific DNA-interstrand crosslinks by bizelesin in genomic and simian virus 40 DNA.

Bizelesin is a bifunctional AT-specific DNA alkylating drug. Our study characterized the ability of bizelesin to induce interstrand crosslinks, a potential lethal lesion. In genomic DNA of BSC-1 cells, bizelesin formed from approx. 0.3 to 6.03+/-0.85 interstrand crosslinks per 106 base pairs, at 5-100 nM drug concentration, respectively, comparable to the number of total adducts previously determined in the same system (J.M. Woynarowski, M.M. McHugh, L.S. Gawron, T.A. Beerman, Biochemistry 34 (1995) 13042-13050). Bizelesin did not induce DNA-protein crosslinks or strand breaks. A model defined target, intracellular simian virus 40 (SV40) DNA, was employed to map at the nucleotide level sites of bizelesin adducts, including potential interstrand crosslinks. Preferential adduct formation was observed at AT tracts which are abundant in the SV40 matrix associated region and the origin of replication. Many sites, including each occurrence of 5'-T(A/T)4A-3', co-mapped on both DNA strands suggesting interstrand crosslinks, although monoadducts were also formed. Bizelesin adducts in naked SV40 DNA were found at similar sites. The localization of bizelesin-induced crosslinks in AT-rich tracts of replication-related regions is consistent with the potent anti-replicative properties of bizelesin. Given the apparent lack of other types of lesions in genomic DNA, interstrand crosslinks localized in AT-rich tracts, and to some extent perhaps also monoadducts, are likely to be lethal effects of bizelesin.  (+info)

(2/2595) A genetic approach to visualization of multisynaptic neural pathways using plant lectin transgene.

The wiring patterns among various types of neurons via specific synaptic connections are the basis of functional logic employed by the brain for information processing. This study introduces a powerful method of analyzing the neuronal connectivity patterns by delivering a tracer selectively to specific types of neurons while simultaneously transsynaptically labeling their target neurons. We developed a novel genetic approach introducing cDNA for a plant lectin, wheat germ agglutinin (WGA), as a transgene under the control of specific promoter elements. Using this method, we demonstrate three examples of visualization of specific transsynaptic neural pathways: the mouse cerebellar efferent pathways, the mouse olfactory pathways, and the Drosophila visual pathways. This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system.  (+info)

(3/2595) Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs.

Use of the differential display technique to isolate progestin-regulated genes in T-47D human breast cancer cells led to identification of a novel gene, EDD. The cDNA sequence contains a 2799 amino acid open reading frame sharing 40% identity with the predicted 2894 amino acid product of the Drosophila melanogaster tumor suppressor gene hyperplastic discs, while the carboxy-terminal 889 amino acids show 96% identity to a rat 100 kDa HECT domain protein. EDD mRNA was progestin-induced in T-47D cells and was highly abundant in testes and expressed at moderately high levels in other tissues, suggesting a broad role for EDD. Anti-EDD antibodies immunoprecipitated an approximately 300 kDa protein from T-47D cell lysates. HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. EDD is likely to function as an E3 as in vitro translated protein bound ubiquitin reversibly through a conserved HECT domain cysteine residue. EDD was localized by FISH to chromosome 8q22, a locus disrupted in a variety of cancers. Given the homology between EDD and the hyperplastic discs protein, which is required for control of imaginal disc growth in Drosophila, EDD potentially has a role in regulation of cell proliferation or differentiation.  (+info)

(4/2595) Direct selection for mutators in Escherichia coli.

We have constructed strains that allow a direct selection for mutators of Escherichia coli on a single plate medium. The plate selection is based on using two different markers whose reversion is enhanced by a given mutator. Plates containing limiting amounts of each respective nutrient allow the growth of ghost colonies or microcolonies that give rise to full-size colonies only if a reversion event occurs. Because two successive mutational events are required, mutator cells are favored to generate full-size colonies. Reversion of a third marker allows direct visualization of the mutator phenotype by the large number of blue papillae in the full-size colonies. We also describe plate selections involving three successive nutrient markers followed by a fourth papillation step. Different frameshift or base substitution mutations are used to select for mismatch-repair-defective strains (mutHLS and uvrD). We can detect and monitor mutator cells arising spontaneously, at frequencies lower than 10(-5) in the population. Also, we can measure a mutator cascade, in which one type of mutator (mutT) generates a second mutator (mutHLS) that then allows stepwise frameshift mutations. We discuss the relevance of mutators arising on a single medium as a result of cells overcoming successive growth barriers to the development and progression of cancerous tumors, some of which are mutator cell lines.  (+info)

(5/2595) Karyotyping of human oocytes by chromosomal analysis of the second polar bodies.

This paper describes a method for obtaining metaphase chromosomes from human second polar bodies. The second polar body nucleus was injected into the cytoplasm of an enucleated oocyte, which is activated shortly after injection. When the polar body nucleus is transformed into a haploid pronucleus, treatment with okadaic acid was used to induce premature chromosome condensation. A total of 25 analysable chromosome plates were obtained from 38 polar bodies karyotyped using this technique. Whole chromosome painting was used to detect second polar bodies (and respectively, oocytes) with unbalanced translocations. In combination with the first polar body analysis, this technique may be useful in preimplantation genetic diagnosis for patients carrying maternal translocations.  (+info)

(6/2595) Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila.

In many organisms, there are multiple isoforms of cytoplasmic dynein heavy chains, and division of labor among the isoforms would provide a mechanism to regulate dynein function. The targeted disruption of somatic genes in Tetrahymena thermophila presents the opportunity to determine the contributions of individual dynein isoforms in a single cell that expresses multiple dynein heavy chain genes. Substantial portions of two Tetrahymena cytoplasmic dynein heavy chain genes were cloned, and their motor domains were sequenced. Tetrahymena DYH1 encodes the ubiquitous cytoplasmic dynein Dyh1, and DYH2 encodes a second cytoplasmic dynein isoform, Dyh2. The disruption of DYH1, but not DYH2, resulted in cells with two detectable defects: 1) phagocytic activity was inhibited, and 2) the cells failed to distribute their chromosomes correctly during micronuclear mitosis. In contrast, the disruption of DYH2 resulted in a loss of regulation of cell size and cell shape and in the apparent inability of the cells to repair their cortical cytoskeletons. We conclude that the two dyneins perform separate tasks in Tetrahymena.  (+info)

(7/2595) Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.

Disruption of newly identified genes in the pathogen Candida albicans is a vital step in determination of gene function. Several gene disruption methods described previously employ long regions of homology flanking a selectable marker. Here, we describe disruption of C. albicans genes with PCR products that have 50 to 60 bp of homology to a genomic sequence on each end of a selectable marker. We used the method to disrupt two known genes, ARG5 and ADE2, and two sequences newly identified through the Candida genome project, HRM101 and ENX3. HRM101 and ENX3 are homologous to genes in the conserved RIM101 (previously called RIM1) and PacC pathways of Saccharomyces cerevisiae and Aspergillus nidulans. We show that three independent hrm101/hrm101 mutants and two independent enx3/enx3 mutants are defective in filamentation on Spider medium. These observations argue that HRM101 and ENX3 sequences are indeed portions of genes and that the respective gene products have related functions.  (+info)

(8/2595) Oxidative stress can activate the epidermal platelet-activating factor receptor.

Platelet-activating factor (1-alkyl-2-acetyl-glycero-phosphocholine) is a lipid mediator that has been implicated in keratinocyte function and cutaneous inflammation. Keratinocytes both synthesize platelet-activating factor and express functional platelet-activating factor receptors linked to calcium mobilization. Oxidative stress to various cells including keratinocytes can also result in the mobilization of intracellular Ca2+, a known stimulus for platelet-activating factor biosynthesis. The ability of the epidermal platelet-activating factor receptors to modulate oxidant-induced signaling was investigated using a unique model system created by retroviral-mediated transduction of the platelet-activating factor receptor-negative epithelial cell line KB with the platelet-activating factor receptor. Treatment of KB cells with the lipid pro-oxidant tert-butyl hydroperoxide induced transient increases in intracellular Ca2+ in a concentration-dependent fashion. Expression of the platelet-activating factor receptor in KB cells lowered the threshold for tert-butyl hydroperoxide-induced Ca2+ flux by an order of magnitude (10 microM in control KB versus 1 microM in KB cells expressing the platelet-activating factor receptors) and increased the peak change in intracellular Ca2+ concentration in response to this lipid hydroperoxide. This augmentation of tert-butyl hydroperoxide-induced Ca2+ mobilization was inhibited by pretreatment with the two competitive platelet-activating factor receptor antagonists CV-6209 and WEB 2086, as well as by the antioxidants vitamin E and 1,1,3,3-tetramethyl-2-thiourea. KB cells synthesized platelet-activating factor and the platelet-activating factor receptor agonist 1-palmitoyl-2-acetyl-glycero-phosphocholine in response to tert-butyl hydroperoxide treatment, suggesting the augmentation of oxidative stress-induced signaling seen in platelet-activating factor receptor-expressing cells was due in part to endogenous platelet-activating factor biosynthesis. These studies suggest involvement of the epidermal platelet-activating factor receptors in oxidant-mediated signaling.  (+info)



  • diseases
  • The Chinese have developed many techniques that can help cure many diseases such as infertility. (discovermagazine.com)
  • The new map will help disease geneticists working to map genetic diseases in African Americans because it provides a more accurate understanding of recombination rates among that population, said the senior author of the research, John Novembre, a UCLA assistant professor of ecology and evolutionary biology and of bioinformatics. (bio-medicine.org)
  • It is now available on the NHS for genetic diseases that are licensed by the Human Fertilisation and Embryology Authority. (ivf.net)
  • chromosome
  • In the 1970's came the discovery of a staining technique called G-banding, from the Giemsa dye, which made the identification of each chromosome much easier with characteristic darker and lighter bands providing the equivalent of latitudes and serving as rough landmarks on the chromosomes. (powerofthegene.com)
  • markers
  • This technique could be used to analyse very large families to determine which individuals inherited a disease in combination with one of the many different markers on different chromosomes. (powerofthegene.com)
  • Thus, RAPD markers offer a potentially simple, rapid and reliable method to evaluate genetic diversity analysis in rice genotypes and to find out diversified parental stocks or traits for conservation which need to be used in future to develop new desirable traits or genotypes. (jacketflap.com)
  • Scientists
  • Scientists are increasingly developing new techniques to investigate the structure and function of DNA. (powerofthegene.com)
  • The good news: By combining the DNA of parents with genetic material from a third person, scientists might have developed a way for women with rare genetic disorders to have healthy children. (discovermagazine.com)
  • The resulting embryo would carry DNA from three parents, and to prove the technique could work in the clinic, scientists would have to try the technique in healthy human embryos - a task that would be "impossible" due to the associated ethical issues [ The Scientist ] , argues researcher Jun-Ichi Hayashi, who wasn't part of the project. (discovermagazine.com)
  • The life scientists used an innovative method involving population genetic models in which they scanned the individual genomes of 2,565 African Americans, as well as 299 African Caribbeans, to study where in the genome each had African ancestry, where they had European ancestry, and where the "switch points" were that mark the location where the ancestry of a DNA segment changes. (bio-medicine.org)
  • Genetic Nomenclature and Quantitative Techniques for Modern Genome Epidemiologists, Clinicians, Educators and other Behavioral Scientists. (igi-global.com)
  • Restriction enzymes are absolutely essential to the work of genetic e ngineers because they allow scientists to cut the sugar - phosphate "backbones" of DNA molecules precisely they way they would like. (techylib.com)
  • involves
  • The technique involves using DNA from Mrs Meagu, her husband Gabriel, and another family member, to compare roughly 300,000 different points across the genome called SNPs (single nucleotide polymorphisms). (ivf.net)
  • differences
  • Genetic differences between humans and mice that may account for these different aging rates include differences in efficiency of DNA repair, antioxidant defenses, energy metabolism, proteostasis maintenance, and recycling mechanisms such as autophagy. (wikipedia.org)
  • material
  • Polymerase chain reaction (PCR), developed by Kary Mullis in 1983, allowed small sections of DNA to be amplified and aided identification and isolation of genetic material. (wikipedia.org)
  • analysis
  • Having a way of continuously monitoring and guiding the process can lead to faster and cheaper results from genetic analysis and reduce the size of the machines used. (medgadget.com)
  • This technique is rapid, because many side chains are analyzed simultaneously and the need for protein purification and biophysical analysis is circumvented. (wikipedia.org)
  • studies
  • Jan YN, Jan LY (1982) Genetic and immunological studies of the nervous system of Drosophila melanogaster. (springer.com)
  • 32,34,27 Studies have shown that with pertinence to genetic editing, the size of the particle for delivery is relevant, with nanoscale design allowing for the development of many different particles with a varied range of specifications. (sciencers.org)
  • work
  • A team including Carnegies Martin Jonikas developed a highly sophisticated tool that will transform the work of plant geneticists by making large-scale genetic characterization of Chlamydomonas mutants possible for the first time. (bio-medicine.org)
  • Since the delitto perfetto technique is based on homologous recombination, this process must be functional in the cells for the technique to work. (wikipedia.org)
  • However
  • However, in most cases our understanding of these spatial patterns of natural variation at a genetic level is limited. (dtu.dk)
  • However, despite the major strides that have been made in genetic understanding, the ability to feasibly make genetic changes still remains out of reach. (sciencers.org)
  • important
  • Genetic diversity plays an important role in plant breeding because hybrids between lines of diverse origin generally display a greater heterosis than those between closely related strains. (jacketflap.com)
  • function
  • Our genetic code, or DNA, is like an orchestra - it contains all of the elements we need to function - but the epigenetic code is essentially the conductor, telling which instruments to play or stay silent, or how to respond at any given moment," Associate Professor Hughes says. (innovations-report.com)
  • uses
  • Another technique uses evolutionary pressures such as breeding from only older members or altering levels of extrinsic mortality. (wikipedia.org)
  • role
  • This technique can also be used to determine whether the side chain of a specific residue plays a significant role in bioactivity. (wikipedia.org)
  • health
  • This technique was developed by a group at the National Institute of Environmental Health Sciences (NIEHS) composed of Michael A. Resnick, Francesca Storici (now at Georgia Institute of Technology), and L. Kevin Lewis (now at Southwest Texas State University). (wikipedia.org)