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Genetic Techniques: Chromosomal, biochemical, intracellular, and other methods used in the study of genetics.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genes, Bacterial: The functional hereditary units of BACTERIA.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

*  Genetic Techniques - The Power of The Gene
... but since no actual tissues are sampled the technique cannot include genetic testing. ... Genetic fingerprinting is also employed in criminal cases to prove that the DNA found at a crime scene is statistically likely ... This non-invasive technique has been used to diagnose Down Syndrome.. The practice of prenatal diagnosis has recently come ... This technique could be used to analyse very large families to determine which individuals inherited a disease in combination ...
  http://powerofthegene.com/genetic-techniques/
*  genetic techniques - Everything2.com
laboratory techniques. Genetic Sequence Comparisons. Microinjection. Using the closest available aerosol product as bug spray. ... and other scientists are generally interested in using one of three organisms with genetic techniques: yeast, transgenic mice, ... An example of this might be the study of a genetic disease like Lesch Nyhan's disease and the recognition that only men have ... or removal of a part of the genetic material the codes for a protein). For example, transgenic mice that have had their Myosin ...
  https://everything2.com/title/genetic+techniques
*  Genetic techniques have role in future of dental care
... 06.03.2014. A visit to the dentist could one day require a detailed look ... "Our genetic code, or DNA, is like an orchestra - it contains all of the elements we need to function - but the epigenetic code ... We can't change the underlying genetic code, but we may be able to change when genes are switched on and off," he says. ... Professor Hughes is part of a team of researchers at the University of Adelaide that has been studying the underlying genetic ...
  http://www.innovations-report.com/html/reports/life-sciences/genetic-techniques-have-role-in-future-of-dental-care.html
*  IDEALS @ Illinois: Sequential Circuit Test Generation Using Genetic Techniques
Sequential Circuit Test Generation Using Genetic Techniques. Welcome to the IDEALS Repository. ...
  https://www.ideals.illinois.edu/handle/2142/81208
*  Recent Advances in Genetic Technique of Microbial Report Cells and Their Applications in Cell Arrays
... Do Hyun Kim,1 Moon Il Kim ... A. Waterworth, A. Hanby, and V. Speirs, "A novel cell array technique for high-throughput, cell-based analysis," In Vitro ... Recent research into genetic engineering of reporter cells, technologies for patterning live cells on solid surfaces, and their ... 2. Genetic Engineering of Microbial Reporter Cells. Although unmodified bacteria have been used as biosensors, based on changes ...
  https://www.hindawi.com/journals/bmri/2015/182107/
*  Recent Advances in Genetic Technique of Microbial Report Cells and Their Applications in Cell Arrays
... Do Hyun Kim,1 Moon Il Kim ... A. Waterworth, A. Hanby, and V. Speirs, "A novel cell array technique for high-throughput, cell-based analysis," In Vitro ... S. K. Srivastava, P. R. Lambadi, T. Ghosh, R. Pathania, and N. K. Navani, "Genetic regulation of spy gene expression in ... B. Wang, R. I. Kitney, N. Joly, and M. Buck, "Engineering modular and orthogonal genetic logic gates for robust digital-like ...
  https://www.hindawi.com/journals/bmri/2015/182107/ref/
*  The new age reproductive genetic techniques to drastically increase IVF success rate
... 01 November 2017 , Views ... The new age reproductive genetic techniques would drastically increase IVF success rates. ... Pre implantation Genetic Screening (PGS) is highly recommended for these couples. PGS along with MitoScore enables selection of ... This artificial or assisted technique of bestowing parenthood has brought a smile on millions of faces. The couples who fail to ...
  https://www.biospectrumindia.com/views/81/9758/the-new-age-reproductive-genetic-techniques-to-drastically-increase-ivf-success-rate.html
*  FishPopTrace: a new genetic technique for fisheries monitoring and the identification of IUU - DTU Orbit
FishPopTrace: a new genetic technique for fisheries monitoring and the identification of IUU. Publication: Research › ... However, many previous genetic studies have observed weak genetic structure in marine fish and, combined with a pelagic larval ... Low genetic and phenotypic divergence in a contact zone between freshwater and marine sticklebacks: gene flow constrains ... However, in most cases our understanding of these spatial patterns of natural variation at a genetic level is limited. For ...
  http://orbit.dtu.dk/en/publications/fishpoptrace-a-new-genetic-technique-for-fisheries-monitoring-and-the-identification-of-iuu
*  Powerful Genetic Engineering Technique Could Modify Entire Wild Populations
By combining two techniques-CRISPR and gene drives-scientists are proposing a system that could change nearly any sexually ... By combining two techniques-CRISPR and gene drives-scientists are proposing a system that could change nearly any sexually ... Scientists Cautiously OK the Next Genetic Revolution. Posted by Kelsey Houston-Edwards and Tim De Chant on Wed, 08 Jun 2016. ... "I don't care if it's a weed or a blight, people still are going to say this is way too massive a genetic engineering project," ...
  http://www.pbs.org/wgbh/nova/next/evolution/crispr-gene-drives/?utm_source=facebook&utm_medium=pbsofficial&utm_campaign=nova_next
*  Genetic engineering techniques - Wikipedia
... another method to deliver genetic modifications to plants List of genetic engineering software: software to code the genetic ... Corinne A. Michels (2002). "7". Genetic Techniques for Biological Research: A Case Study Approach. John Wiley & Sons. pp. 85-88 ... "The process of genetic modification". Nicholl, Desmond S. T. (2008-06-23). An Introduction to Genetic Engineering (third ed.). ... Knowledge of how the gene functions and interacts with other genetic elements is important. Genetic screens can be carried out ...
  https://en.wikipedia.org/wiki/Genetic_engineering_techniques
*  Algae-based product producer develops genetic optimization technique
This technology, homologous recombination, enables genetic optimization without introducing foreign genes, thereby preserving ... Algae-based product producer Aurora Algae has developed a new technique for genetic optimization to create advanced-trait algae ... Algae-based product producer Aurora Algae has developed a new technique for genetic optimization to create advanced-trait algae ... Danes to test breeding 'social pigs' to optimize genetic progress. Related Products. Antibiotic-Free Poultry Production. Top US ...
  https://www.wattagnet.com/articles/11368-algae-based-product-producer-develops-genetic-optimization-technique
*  New technique overcomes genetic cause of infertility | EurekAlert! Science News
... offering a potential new approach to tackling a common genetic cause of human infertility. ... New technique overcomes genetic cause of infertility. The Francis Crick Institute. Journal. Science. Funder. Francis Crick ... offering a potential new approach to tackling a common genetic cause of human infertility. ... that are infertile to have children through assisted reproduction using this technique. ...
  https://www.eurekalert.org/pub_releases/2017-08/tfci-nto081417.php
*  Groundbreaking New CRISPR Technique Reverses Genetic Disease Symptoms in Mice
The technique uses two adeno-associated viruses (AAV) to introduce genetic manipulation machinery to cells in post-natal mice. ... However, the technique also has the potential to create genetic mutations that may produce undesired side effects. ... In tests using a mouse model of acute kidney disease, the technique was seen to activate damaged or silenced genes, allowing ... Researchers hope the technique could lend itself to developing treatments for a wide variety of neurological disorders, ...
  https://www.sciencealert.com/this-new-crispr-technique-reverses-diseases-in-mice
*  Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System | SpringerLink
... and genetic fluorescent labeling have become standard techniques to study the anatomy, function, and development of the ... Immunofluorescence (IF ) and genetic fluorescent labeling have become standard techniques to study the anatomy, function, and ... Selcho M., Wegener C. (2015) Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System. ... Kao C-F, Lee T (2012) In vivo single cell labeling techniques. Neuromethods 69:91-124CrossRefGoogle Scholar ...
  https://link.springer.com/protocol/10.1007/978-1-4939-2313-7_2
*  Improvement of Rice Genotypes Through Molecular Marker Technique: Genetic Diversity Analysis via RAPD markers - by MD...
Genetic Diversity Analysis via RAPD markers - Rice, Oryza sativa (2n=24) belon ... About Improvement of Rice Genotypes Through Molecular Marker Technique: ... These are the most recent 10 blog posts about Improvement of Rice Genotypes Through Molecular Marker Technique: Genetic ... PCR-based Random Amplified Polymorphic DNA (RAPD) technique was used for assessing genetic diversity and relationships among ...
  https://www.jacketflap.com/bookdetail.asp?bookid=3659159395
*  Innovative genetic and cellular techniques help identify multiple disease targets - BioPortfolio.com
Innovative genetic, cellular techniques help identify multiple disease targets *Scientists discover genetic markers for severe ... Innovative genetic and cellular techniques help identify multiple disease targets. 19:00 EST 11 Nov 2017 , AAAS ... More From BioPortfolio on "Innovative genetic and cellular techniques help identify multiple disease targets". *Related ... Original Article: Innovative genetic and cellular techniques help identify multiple disease targets ...
  https://www.bioportfolio.com/news/article/3414969/Innovative-genetic-and-cellular-techniques-help-identify-multiple-disease-targets.html
*  CRISPR: A game-changing genetic engineering technique - Science in the News
67 thoughts on "CRISPR: A game-changing genetic engineering technique" * Paul Harvey says: ... CRISPR: A game-changing genetic engineering technique. Have you heard? A revolution has seized the scientific community. Within ... Compared to previous techniques for modifying DNA, this new approach is much faster and easier. This technology is referred to ... No, it's not a robot or the latest dating app, it's a method for genetic manipulation that is rewriting the way we change DNA. ...
  http://sitn.hms.harvard.edu/flash/2014/crispr-a-game-changing-genetic-engineering-technique/?replytocom=240036
*  Magrudy.com - Techniques in Genetic Engineering ‬
Techniques in Genetic Engineering briefly introduces some common genetic engineering techniques and focuses on how to approach ... this book-Techniques in Genetic Engineering-IS NOT: a laboratory manual; nor is it a textbook on molecular biology or ... This easy-to-follow book presents not only the theoretical background of molecular techniques, but also provides case study ... Although not an exhaustive review of these techniques, basic information includes core concepts such as DNA, RNA, protein, ...
  https://magrudy.com/book/techniques-in-genetic-engineering-9781482260892/
*  Controversial Technique Could Prevent Genetic Disease by Making 3-Parent Babies - 80beats : 80beats
Controversial Technique Could Prevent Genetic Disease by Making 3-Parent Babies. By Andrew Moseman , April 14, 2010 3:36 pm ... This technique was pioneered in monkeys last summer, but researchers have now done a proof-of-principle study with human cells. ... This technique has proven effective for many and there is evidence to prove it. Acupuncture treatment is more effective if the ... The good news: By combining the DNA of parents with genetic material from a third person, scientists might have developed a way ...
  http://blogs.discovermagazine.com/80beats/2010/04/14/controversial-technique-could-prevent-genetic-disease-by-making-3-parent-babies/
*  New technique will accelerate genetic characterization of phot... ( Stanford CA Photosynthesis provides fix...)
P,New,technique,will,accelerate,genetic,characterization,of,photosynthesis,biological,biology news articles,biology news today, ... New technique sheds light on human neural networks. 5. New technique for identifying gene-enhancers. 6. Heat-based technique ... New technique will accelerate genetic characterization of photosynthesis. Stanford CA Photosynthesis provides fixed carbon and ... New technique makes biogasoline from plant waste. 9. Facelift complications eased with help of new 3-D imaging technique. 10. ...
  http://www.bio-medicine.org/biology-news-1/New-technique-will-accelerate-genetic-characterization-of-photosynthesis-35173-1/
*  Development of Innovative Research Technique in Genetic Epidemiology of Malaria and Other Parasitic Diseases in Lao PDR for...
Development of Innovative Research Technique in Genetic Epidemiology of Malaria and Other Parasitic Diseases in Lao PDR for ... Molecular and genetic monitoring of parasitic diseases that threaten the people of Laos. The emergence and spread of drug- ... This project involves working with young Laotian researchers on molecular genetic epidemiology for these three significant ... parasitic diseases, with the aim of developing simple, rapid and precise diagnostic methods using LAMP techniques. The ...
  http://www.jst.go.jp/global/english/kadai/h2508_laos.html
*  Emerging Techniques in Protein and Genetic Engineering
Extensive discussion of data, data analysis and application of theories and techniques to the current work of course ... Core Techniques in Protein and Genetic Engineering. *Emerging Techniques in Protein and Genetic Engineering ... Core Techniques in Protein and Genetic Engineering. *Emerging Techniques in Protein and Genetic Engineering ...
  https://www.btci.org/scientific-courses/emerging-techniques-in-protein-and-genetic-engineering/
*  A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT | Genetics
A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT. Reed C. Rollins ... A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT. Reed C. Rollins ... A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT. Reed C. Rollins ... A NOTE ON SOME TECHNIQUES OF OBTAINING GENETIC SEGREGATION IN APOMICTIC STRAINS OF THE GUAYULE RUBBER PLANT ...
  http://www.genetics.org/content/36/5/435
*  New Technique Greatly Increases Sensitivity of Genetic Testing | HHMI.org
A new laboratory method invented by HHMI investigators and their colleagues improves the accuracy of current genetic diagnostic ... Vogelstein emphasized that the conversion technique must still be refined before it can be introduced into routine genetic ... "The technique is simple enough that it should not add much to the costs of testing for certain diseases if it is implemented in ... The technique will clearly increase the number of individuals for whom we will be able to make a diagnosis." Markowitz and his ...
  https://www.hhmi.org/news/new-technique-greatly-increases-sensitivity-genetic-testing
*  Online Background Checks, US Criminal History Information | Free Warrant Search Delaware
Documentaries are genetic parties or scores that have been copy-and-pasted around the world. While eating at the history of ... Awkwardly, from the political 3 techniques that had been transported to prague, one directly fled to germany. After devin had ... Arrest search pbso decays were established to educate, encourage, and register the disenfranchised genetic rooms. Hamasaki's ...
  http://tenant-credit-check.life/free-warrant-search-delaware.html

(1/2595) Induction of AT-specific DNA-interstrand crosslinks by bizelesin in genomic and simian virus 40 DNA.

Bizelesin is a bifunctional AT-specific DNA alkylating drug. Our study characterized the ability of bizelesin to induce interstrand crosslinks, a potential lethal lesion. In genomic DNA of BSC-1 cells, bizelesin formed from approx. 0.3 to 6.03+/-0.85 interstrand crosslinks per 106 base pairs, at 5-100 nM drug concentration, respectively, comparable to the number of total adducts previously determined in the same system (J.M. Woynarowski, M.M. McHugh, L.S. Gawron, T.A. Beerman, Biochemistry 34 (1995) 13042-13050). Bizelesin did not induce DNA-protein crosslinks or strand breaks. A model defined target, intracellular simian virus 40 (SV40) DNA, was employed to map at the nucleotide level sites of bizelesin adducts, including potential interstrand crosslinks. Preferential adduct formation was observed at AT tracts which are abundant in the SV40 matrix associated region and the origin of replication. Many sites, including each occurrence of 5'-T(A/T)4A-3', co-mapped on both DNA strands suggesting interstrand crosslinks, although monoadducts were also formed. Bizelesin adducts in naked SV40 DNA were found at similar sites. The localization of bizelesin-induced crosslinks in AT-rich tracts of replication-related regions is consistent with the potent anti-replicative properties of bizelesin. Given the apparent lack of other types of lesions in genomic DNA, interstrand crosslinks localized in AT-rich tracts, and to some extent perhaps also monoadducts, are likely to be lethal effects of bizelesin.  (+info)

(2/2595) A genetic approach to visualization of multisynaptic neural pathways using plant lectin transgene.

The wiring patterns among various types of neurons via specific synaptic connections are the basis of functional logic employed by the brain for information processing. This study introduces a powerful method of analyzing the neuronal connectivity patterns by delivering a tracer selectively to specific types of neurons while simultaneously transsynaptically labeling their target neurons. We developed a novel genetic approach introducing cDNA for a plant lectin, wheat germ agglutinin (WGA), as a transgene under the control of specific promoter elements. Using this method, we demonstrate three examples of visualization of specific transsynaptic neural pathways: the mouse cerebellar efferent pathways, the mouse olfactory pathways, and the Drosophila visual pathways. This strategy should greatly facilitate studies on the anatomical and functional organization of the developing and mature nervous system.  (+info)

(3/2595) Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs.

Use of the differential display technique to isolate progestin-regulated genes in T-47D human breast cancer cells led to identification of a novel gene, EDD. The cDNA sequence contains a 2799 amino acid open reading frame sharing 40% identity with the predicted 2894 amino acid product of the Drosophila melanogaster tumor suppressor gene hyperplastic discs, while the carboxy-terminal 889 amino acids show 96% identity to a rat 100 kDa HECT domain protein. EDD mRNA was progestin-induced in T-47D cells and was highly abundant in testes and expressed at moderately high levels in other tissues, suggesting a broad role for EDD. Anti-EDD antibodies immunoprecipitated an approximately 300 kDa protein from T-47D cell lysates. HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. EDD is likely to function as an E3 as in vitro translated protein bound ubiquitin reversibly through a conserved HECT domain cysteine residue. EDD was localized by FISH to chromosome 8q22, a locus disrupted in a variety of cancers. Given the homology between EDD and the hyperplastic discs protein, which is required for control of imaginal disc growth in Drosophila, EDD potentially has a role in regulation of cell proliferation or differentiation.  (+info)

(4/2595) Direct selection for mutators in Escherichia coli.

We have constructed strains that allow a direct selection for mutators of Escherichia coli on a single plate medium. The plate selection is based on using two different markers whose reversion is enhanced by a given mutator. Plates containing limiting amounts of each respective nutrient allow the growth of ghost colonies or microcolonies that give rise to full-size colonies only if a reversion event occurs. Because two successive mutational events are required, mutator cells are favored to generate full-size colonies. Reversion of a third marker allows direct visualization of the mutator phenotype by the large number of blue papillae in the full-size colonies. We also describe plate selections involving three successive nutrient markers followed by a fourth papillation step. Different frameshift or base substitution mutations are used to select for mismatch-repair-defective strains (mutHLS and uvrD). We can detect and monitor mutator cells arising spontaneously, at frequencies lower than 10(-5) in the population. Also, we can measure a mutator cascade, in which one type of mutator (mutT) generates a second mutator (mutHLS) that then allows stepwise frameshift mutations. We discuss the relevance of mutators arising on a single medium as a result of cells overcoming successive growth barriers to the development and progression of cancerous tumors, some of which are mutator cell lines.  (+info)

(5/2595) Karyotyping of human oocytes by chromosomal analysis of the second polar bodies.

This paper describes a method for obtaining metaphase chromosomes from human second polar bodies. The second polar body nucleus was injected into the cytoplasm of an enucleated oocyte, which is activated shortly after injection. When the polar body nucleus is transformed into a haploid pronucleus, treatment with okadaic acid was used to induce premature chromosome condensation. A total of 25 analysable chromosome plates were obtained from 38 polar bodies karyotyped using this technique. Whole chromosome painting was used to detect second polar bodies (and respectively, oocytes) with unbalanced translocations. In combination with the first polar body analysis, this technique may be useful in preimplantation genetic diagnosis for patients carrying maternal translocations.  (+info)

(6/2595) Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila.

In many organisms, there are multiple isoforms of cytoplasmic dynein heavy chains, and division of labor among the isoforms would provide a mechanism to regulate dynein function. The targeted disruption of somatic genes in Tetrahymena thermophila presents the opportunity to determine the contributions of individual dynein isoforms in a single cell that expresses multiple dynein heavy chain genes. Substantial portions of two Tetrahymena cytoplasmic dynein heavy chain genes were cloned, and their motor domains were sequenced. Tetrahymena DYH1 encodes the ubiquitous cytoplasmic dynein Dyh1, and DYH2 encodes a second cytoplasmic dynein isoform, Dyh2. The disruption of DYH1, but not DYH2, resulted in cells with two detectable defects: 1) phagocytic activity was inhibited, and 2) the cells failed to distribute their chromosomes correctly during micronuclear mitosis. In contrast, the disruption of DYH2 resulted in a loss of regulation of cell size and cell shape and in the apparent inability of the cells to repair their cortical cytoskeletons. We conclude that the two dyneins perform separate tasks in Tetrahymena.  (+info)

(7/2595) Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions.

Disruption of newly identified genes in the pathogen Candida albicans is a vital step in determination of gene function. Several gene disruption methods described previously employ long regions of homology flanking a selectable marker. Here, we describe disruption of C. albicans genes with PCR products that have 50 to 60 bp of homology to a genomic sequence on each end of a selectable marker. We used the method to disrupt two known genes, ARG5 and ADE2, and two sequences newly identified through the Candida genome project, HRM101 and ENX3. HRM101 and ENX3 are homologous to genes in the conserved RIM101 (previously called RIM1) and PacC pathways of Saccharomyces cerevisiae and Aspergillus nidulans. We show that three independent hrm101/hrm101 mutants and two independent enx3/enx3 mutants are defective in filamentation on Spider medium. These observations argue that HRM101 and ENX3 sequences are indeed portions of genes and that the respective gene products have related functions.  (+info)

(8/2595) Oxidative stress can activate the epidermal platelet-activating factor receptor.

Platelet-activating factor (1-alkyl-2-acetyl-glycero-phosphocholine) is a lipid mediator that has been implicated in keratinocyte function and cutaneous inflammation. Keratinocytes both synthesize platelet-activating factor and express functional platelet-activating factor receptors linked to calcium mobilization. Oxidative stress to various cells including keratinocytes can also result in the mobilization of intracellular Ca2+, a known stimulus for platelet-activating factor biosynthesis. The ability of the epidermal platelet-activating factor receptors to modulate oxidant-induced signaling was investigated using a unique model system created by retroviral-mediated transduction of the platelet-activating factor receptor-negative epithelial cell line KB with the platelet-activating factor receptor. Treatment of KB cells with the lipid pro-oxidant tert-butyl hydroperoxide induced transient increases in intracellular Ca2+ in a concentration-dependent fashion. Expression of the platelet-activating factor receptor in KB cells lowered the threshold for tert-butyl hydroperoxide-induced Ca2+ flux by an order of magnitude (10 microM in control KB versus 1 microM in KB cells expressing the platelet-activating factor receptors) and increased the peak change in intracellular Ca2+ concentration in response to this lipid hydroperoxide. This augmentation of tert-butyl hydroperoxide-induced Ca2+ mobilization was inhibited by pretreatment with the two competitive platelet-activating factor receptor antagonists CV-6209 and WEB 2086, as well as by the antioxidants vitamin E and 1,1,3,3-tetramethyl-2-thiourea. KB cells synthesized platelet-activating factor and the platelet-activating factor receptor agonist 1-palmitoyl-2-acetyl-glycero-phosphocholine in response to tert-butyl hydroperoxide treatment, suggesting the augmentation of oxidative stress-induced signaling seen in platelet-activating factor receptor-expressing cells was due in part to endogenous platelet-activating factor biosynthesis. These studies suggest involvement of the epidermal platelet-activating factor receptors in oxidant-mediated signaling.  (+info)



  • transgenic
  • These include: Molecular and biochemical techniques Electrophoresis and blotting Immunostaining Chromatography Mass spectrometry PCR and sequencing Microarrays Imaging technologies Light, fluorescence, and electron microscopy MRI PET X-ray Genetic engineering/modification Transfection Viral transduction Transgenic model organisms Electrophysiology techniques Patch clamp EEG, EKG, ERG In silico techniques Bioinformatics Computational biology Biomedical scientists typically obtain a bachelor of science degree, and usually take postgraduate studies leading to a diploma, master or doctorate. (wikipedia.org)
  • chromosome
  • In the 1970's came the discovery of a staining technique called G-banding, from the Giemsa dye, which made the identification of each chromosome much easier with characteristic darker and lighter bands providing the equivalent of latitudes and serving as rough landmarks on the chromosomes. (powerofthegene.com)
  • humans
  • Humans of course make excellent animal models, but are horrible for molecular genetics because everything is un ethical , and for similar reasons, less useful than animals that you can drug, cut open, and cross with careful predetermined precision for classical genetics techniques. (everything2.com)
  • If the findings can be safely transferred into humans, it might eventually be possible for men with Klinefelter syndrome (XXY) or Double Y syndrome (XYY) that are infertile to have children through assisted reproduction using this technique. (eurekalert.org)
  • That said, there's still a long way to go before the technique could be tested in humans. (sciencealert.com)
  • primarily
  • Evolutionary biologist Richard Dawkins made groundbreaking headway into the field of cultural transmission with his 1976 book entitled The Selfish Gene, which focused heavily on the move to evolution being understood primarily by genetic influence. (wikipedia.org)
  • However
  • However, in most cases our understanding of these spatial patterns of natural variation at a genetic level is limited. (dtu.dk)
  • However, many previous genetic studies have observed weak genetic structure in marine fish and, combined with a pelagic larval stage, this has supported the hypothesis that gene flow is extensive and that there is little opportunity for differentiation and local adaptation any scale other than macrogeographic. (dtu.dk)
  • analysis
  • Due to the increased need for multiplex, high-throughput analysis capability, cell array techniques have recently garnered significant attention [ 4 ]. (hindawi.com)
  • This technique is rapid, because many side chains are analyzed simultaneously and the need for protein purification and biophysical analysis is circumvented. (wikipedia.org)
  • Genetic techniques capable of providing this sort of information include Restriction Fragment Length Polymorphism (RFLP) analysis and microsatellite analysis. (wikipedia.org)
  • Diverse
  • Genetic diversity plays an important role in plant breeding because hybrids between lines of diverse origin generally display a greater heterosis than those between closely related strains. (jacketflap.com)
  • development
  • Algae-based product producer Aurora Algae has developed a new technique for genetic optimization to create advanced-trait algae strains for product development in the pharmaceutical, nutrition, renewable energy and animal feed markets. (wattagnet.com)
  • role
  • This technique can also be used to determine whether the side chain of a specific residue plays a significant role in bioactivity. (wikipedia.org)
  • future
  • Brain Plague tells of a future in which genetic engineering, combined with nanotechnology can do everything from shaping our bodies to growing enormous buildings for us. (wikipedia.org)
  • function
  • Our genetic code, or DNA, is like an orchestra - it contains all of the elements we need to function - but the epigenetic code is essentially the conductor, telling which instruments to play or stay silent, or how to respond at any given moment," Associate Professor Hughes says. (innovations-report.com)
  • large
  • A team including Carnegies Martin Jonikas developed a highly sophisticated tool that will transform the work of plant geneticists by making large-scale genetic characterization of Chlamydomonas mutants possible for the first time. (bio-medicine.org)
  • include
  • Physical mapping techniques used to generate a gene map include: Restriction mapping, Fluorescent in situ hybridization (FISH), and Sequence tagged site (STS) mapping. (wikipedia.org)
  • predisposition
  • Regarding aetiology of maxillary displaced canines, different theories have been proposed, two primary theories are: the guidance theory and the genetic theory.6 The guidance theory7 suggests that the eruption of the canine is influenced by presence, size, shape and positioning of the maxillary lateral incisor while genetic theory8 suggests that the impaction is due to a genetic predisposition. (thefreelibrary.com)
  • Although these ninja can use their different elements separately with little effort, combining the two elements is impossible for ninja who do not have a genetic predisposition for it. (wikipedia.org)
  • Neural
  • The complementary technique is retrograde tracing, which is used to trace neural connections from their termination to their source (i.e. synapse to cell body). (wikipedia.org)
  • protein
  • Here, we describe a technique that consists of a combination of biochemistry approaches, quantitative proteomics and computational methods using wild‐type and deletion strains to investigate the organization of proteins within macromolecular protein complexes. (embopress.org)
  • The key of our method is the systematic purification of several tagged components of the protein complex in multiple genetic deletion strains, which serve to compromise the integrity of the complex. (embopress.org)
  • In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on its outside while containing the gene for the protein on its inside, resulting in a connection between genotype and phenotype. (wikipedia.org)
  • The technique is also used to determine tumour antigens (for use in diagnosis and therapeutic targeting) and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. (wikipedia.org)
  • see also Viral neuronal tracing) In order to trace projections from a specific region or cell, a genetic construct, virus or protein can be locally injected, after which it is allowed to be transported anterogradely. (wikipedia.org)
  • common
  • Fragile X is the most common cause of inherited intellectual disability and a major genetic factor in autism spectrum disorder. (wisc.edu)
  • With the exception of co-opted ERV loci such as syncytins , which could increase in frequency due to positive selection, we assume ERV loci become common by genetic drift, and the average time for a neutral allele to go to fixation is 4Ne generations (where Ne is the effective population size). (scienceblogs.com)
  • allow
  • Then, they could reliably identify fetal DNA, which would in turn allow them to diagnose certain fetal genetic conditions such as Down Syndrome , which can be detected by observing presence of extra chromosomes. (wikipedia.org)
  • The anterograde and retrograde tracing techniques allow the detailed descriptions of neuronal projections from a single neuron or a defined population of neurons to their various targets throughout the nervous system. (wikipedia.org)
  • determine
  • We applied this technique to determine the organization of two well‐studied complexes, Spt-Ada-Gcn5 histone acetyltransferase (SAGA) and ADA, for which no comprehensive high‐resolution structures exist. (embopress.org)
  • team
  • The time needed to genetically sequence the bacteria causing tuberculosis (Mtb) from patient samples has been reduced from weeks to days using a new technique developed by a UCL-led team. (medicalnewstoday.com)