No data available that match "DNA, Viral"



*  Retroviral mutation rates and A-to-G hypermutations during different stages of retroviral replication.

... by viral reverse transcriptase, or during plus-strand DNA sy ... Retroviruses mutate at a high rate in vivo during viral ... Mutations may occur during proviral transcription by RNA polymerase II, during minus-strand DNA synthesis (RNA template) ... by viral reverse transcriptase, or during plus-strand DNA synthesis (DNA template) by reverse transcriptase. To determine the ... DNA, Viral. Deoxyribonuclease BamHI / metabolism. Deoxyribonuclease HindIII / metabolism. Dogs. Gammaretrovirus / genetics*, ...
biomedsearch.com/nih/Retroviral-mutation-rates-to-G/8892879.html

*  Hepatitis b dna viral load - Answers on HealthTap

Lutwick on hepatitis b dna viral load: and your story stumps many a doctor, often! :) a lot of the recommendations depend on - ... Hbv DNA viral load result: 451.35 iu/ml (1895.67 copies/ml) HBsAG 4.441 and antihbs 0.10. What does this mean? Thank you! ... My hbv viral load is 40 iu/ml, hbv dna load is 233cp/ml, what does it mean. Thank you Dr. ... I just got my result on HBV VIRAL LOAD and is 40 iu/ml HBV DNA LOAD is 233cp/ml. what does this mean. Thank you. ...
https://healthtap.com/topics/hepatitis-b-dna-viral-load

*  Biology-Online • View topic - Viral DNA in Host DNA?

When viral DNA integrates in its host DNA and later cleaves to leave the host cell:. - What initiates the response?. - Does the ... Viral DNA in Host DNA?. Genetics as it applies to evolution, molecular biology, and medical aspects. ... Viral DNA in Host DNA?. by TToe » Thu Nov 22, 2012 1:03 pm ...
biology-online.org/biology-forum/about27684.html

*  Common mechanisms for viral DNA replication ( How DNA replicates is a critical questi...)

DNA ...The structure shows that although T-ag and its functionally analogous ...,Common,mechanisms,for,viral,DNA,replication, ... How DNA replicates is a critical question for understanding life. ... Common mechanisms for viral DNA replication. How DNA replicates is a critical question for understanding life. DNA ...The ... and colleagues report the crystal structure of the DNA-binding domain of SV40 T-ag on a DNA fragment derived from the viral ...
bio-medicine.org/biology-news/Common-mechanisms-for-viral-DNA-replication-4575-1/

*  DNA Viral | IlmuPenyakit.com

Tag: DNA Viral. Reverse Transcriptase Inhibitors. Reverse transcriptase inhibitors (RTI) dapat dibagi dalam dua kelompok, yaitu ...
ilmupenyakit.com/tag/dna-viral

*  Viral DNA sequence a possible trigger for breast cancer ( A small sequence of DNA in the envelope...)

Viral,DNA,sequence,a,possible,trigger,for,breast,cancer,biological,biology news articles,biology news today,latest biology news ... The DNA sequence in question is usually found in immune cells and help...Katz and colleagues now show that this sequence is ... A small sequence of DNA in the envelope (Env) protein of a mouse breas... ... Viral DNA sequence a possible trigger for breast cancer. A small sequence of DNA in the envelope (Env) protein of a mouse breas ...
bio-medicine.org/biology-news/Viral-DNA-sequence-a-possible-trigger-for-breast-cancer-5-1/

*  Magnetic Viral RNA or DNA Isolation

Rapid manual and automated small-scale purification of viral RNA and DNA from cell-free body fluids in a 96-well format. ... Extraction of viral DNA and RNA from serum or plasma *Veterinary testing applications *Norovirus RNA isolation from stool ... Magnetic Bead-Based Isolation of Viral RNA or DNA-NucleoMag 96 Virus. NucleoMag 96 Virus is designed for rapid manual and ... The kit provides reagents for the purification of viral DNA/RNA from up to 200 µl sample volume. ...
clontech.com/US/Products/Nucleic_Acid_Purification/RNA/Viral/Magnetic?sitex=10020:22372:US

*  Otters and Science News: HOW VIRAL DNA IN OUR HUMAN GENOME PROTECTS US FROM INFECTION - HUMAN DNA IS 8% OF VIRAL ORIGIN

HOW VIRAL DNA IN OUR HUMAN GENOME PROTECTS US FROM INFECTION - HUMAN DNA IS 8% OF VIRAL ORIGIN ... 2) DNA specialists have DELIBERATELY disregarded this viral DNA in the human genome. They ignore it because they can't explain ... There has been evidence for some time that some viral DNA living in symbiosis with vertebrate DNA in the human genome plays a ... DNA and RNA. DNA and RNA floating freely in cytoplasm.. DNA or RNA enclosed inside a coat of protein.. ...
ottersandsciencenews.blogspot.com/2016/03/how-viral-dna-in-our-human-genome.html

*  Viral Protein Transforms as it Measures Out DNA - Philadelphia University + Thomas Jefferson University

Viral Protein Transforms as it Measures Out DNA. New insight into two states of a viral protein reveal its function as a DNA- ... "We think that DNA binds to the immature portal protein and wraps around it like a python, as it enters the viral capsid with ... But the picture of how that DNA is loaded into those viral cages, or capsids, was blurry, especially for two of the most common ... "And that it must also adopt other conformations earlier in viral assembly that have the capacity to bind both DNA and other ...
jefferson.edu/university/research/news/viral_protein.html

*  "DNA vaccines for viral infections: basic studies and applications" by Harriet L. Robinson and Tamera Marie Pertmer

Robinson, Harriet L. and Pertmer, Tamera Marie, "DNA vaccines for viral infections: basic studies and applications" (2000). ... Animals; Humans; Immunization; Infant, Newborn; *Vaccines, DNA; *Viral Vaccines; Virus Diseases; Viruses ...
escholarship.umassmed.edu/gsbs_sp/1050/

*  Granulocyte-macrophage colony-stimulating factor enhances vi... : AIDS

Detection of integrated viral DNA. Briefly, genomic DNA from aggregates was extracted using the Qiagen tissue kit (Qiagen, ... the effect of administration of GM-CSF on viral replication was examined; the consequence of exposure of stavudine on viral ... Viral infection of the aggregates. Two weeks after the aggregates were established, they were exposed to 1 ng/ml GM-CSF every ... To grow viral stock, 5 × 106 PBMC/5 ml were maintained in culture for 2 days with phytohaemagglutinin (2 μg/ml) and interleukin ...
journals.lww.com/aidsonline/Fulltext/2002/02150/Granulocyte_macrophage_colony_stimulating_factor.13.aspx

*  A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge (Esox...

DNA vaccine Viral hemorrhagic septicemia virus Fish Background. The emergence of a novel Novirhadovirus, viral hemorrhagic ... A DNA vaccine encoding the viral hemorrhagic septicemia virus genotype IVb glycoprotein confers protection in muskellunge (Esox ... Boudinot P, Blanco M, De Kinkelin P, Benmansour A. Combined DNA immunization with the glycoprotein gene of viral hemorrhagic ... Lorenzen N, Lorenzen E, Einer-Jensen K. Immunity to viral haemorrhagic septicaemia (VHS) following DNA vaccination of rainbow ...
https://virologyj.biomedcentral.com/articles/10.1186/s12985-016-0662-8

*  Analogues of Etoposide for the Treatment of Tumours - CellAct Pharma GmbH

The enzyme topoisomerase II induces transient DNA double strand breaks to enable modifications of DNA tertiary structure. ... Synergistic treatment of DNA viral infections. February, 2006. Hartley. Foreign References:. WO2003048166A1. 2003-06-12. ...
freepatentsonline.com/y2013/0130998.html

*  HEP B - Hepatitis B - MedHelp

3 HBV DNA viral load 10525 4 Ultrasound scan, August 2011 normal ( elastograpy 1.16) 5 Alpha-petoproein 2 (october 2011) I am ... 3 HBV DNA viral load 10525 4 Ultrasound scan, August 2011 normal ( elastograpy 1.16) 5 Alpha-petoproein 2 (october 2011) I am ... First year it was ok but last 2 tests my viral load is above 10.000. My last test results are 1 Hepatitis B virus ('e' antigen ... First year it was ok but last 2 tests my viral load is above 10.000. My last test results are 1 Hepatitis B virus ('e' antigen ...
medhelp.org/posts/Hepatitis-B/HEP-B/show/1927330

*  Plus it

Among 488 cases, 179 and 274 samples with available tumor DNA were successfully assayed for HPV viral DNA using the MPG and ... are positive either for HPV DNA and/or for β-globin DNA); 120 samples of 300 (40%) were negative for both HPV DNA and for β- ... whereas the prevalence of HPV16 DNA (SFPCR) was 28%. The concordance between HPV16 DNA (SFPCR) and HPV16 DNA (MPG) was 53.2% ... Biospecimen collection and detection of viral DNA. Tumor samples from all incident cases where the tissue remained for ...
cancerres.aacrjournals.org/content/72/19/5004

*  Palmitic Acid-Modified Poly- l -Lysine for Non-Viral Delivery of Plasmid DNA to Skin...

Palmitic Acid-Modified Poly- l -Lysine for Non-Viral Delivery of Plasmid DNA to Skin Fibroblasts ...
https://infona.pl/resource/bwmeta1.element.acs-doi-10_1021_bm060940x

*  US1988000986 VIRAL VECTOR SYSTEM FOR THE INTRODUCTION OF FOREIGN DNA EXPRESSION PRODUCTS INTO GALLINACEOUS BIRDS

VIRAL VECTOR SYSTEM FOR THE INTRODUCTION OF FOREIGN DNA EXPRESSION PRODUCTS INTO GALLINACEOUS BIRDS ... EN)Viral vectors capable of delivering and expressing foreign DNA in gallinaceous birds are disclosed. These viral vectors also ... 1. (WO1988007088) VIRAL VECTOR SYSTEM FOR THE INTRODUCTION OF FOREIGN DNA EXPRESSION PRODUCTS INTO GALLINACEOUS BIRDS PCT ... EN) VIRAL VECTOR SYSTEM FOR THE INTRODUCTION OF FOREIGN DNA EXPRESSION PRODUCTS INTO GALLINACEOUS BIRDS. (FR) SYSTEME DE ...
https://patentscope.wipo.int/search/en/detail.jsf?docId=WO1988007088

*  A Comparison of Adefovir and Tenofovir for the Treatment of Lamivudine-Resistant Hepatitis B Virus in People With HIV - Full...

HIV and HBV DNA viral load will be tested every 12 weeks. CD4 cell counts will be tested at Weeks 24, 48, 72, and 96. ... DNA, Viral. Hepatitis B Virus. Adefovir dipivoxil. Tenofovir disoproxil fumarate. Treatment Experienced. ... Serum HBV DNA of 100,000 copies/ml or greater. *Positive for serum hepatitis B surface antigen (HBsAg) within 12 weeks prior to ... DNA Virus Infections. Tenofovir. Lamivudine. Adefovir. Adefovir dipivoxil. Antiviral Agents. Anti-Infective Agents. Reverse ...
https://clinicaltrials.gov/show/NCT00033163?order=94

*  A Phase I Trial of APL 400-003 Vaccine: Safety and Immune Response Evaluations of Multiple Injections at Escalating Doses in...

DNA, Viral. Additional relevant MeSH terms: HIV Infections. Lentivirus Infections. Retroviridae Infections. RNA Virus ... Facilitated DNA inoculation, a new type of DNA vaccine, involves direct injection of non-infectious HIV genes into a patient's ... Facilitated DNA inoculation, a new type of DNA vaccine, involves direct injection of non-infectious HIV genes into a patient's ... Host cells that have taken up these genes then produce viral proteins in a form that elicits immune responses in the form of ...
https://clinicaltrials.gov/show/NCT00002350

*  Fossilized human feces from 14th century contain antibiotic resist... ( A team of French investigators has d...)

A team of French investigators has discovered viruses containing genes... This is the first paper to analyze an ancient DNA ... viral metagenome ...The viruses in the fecal sample are phages which are viruses that inf...The communities of phage within the ... "This is the first paper to analyze an ancient DNA viral metagenome," says Rebecca Vega Thurber of Oregon State University, ... This is the first paper to analyze an ancient DNA viral metagenome ...The viruses in the fecal sample are phages which are ...
bio-medicine.org/biology-news-1/Fossilized-human-feces-from-14th-century-contain-antibiotic-resistance-genes-34356-1/

*  Comprehensive Biophysics - 1st Edition

4.22 Viral DNA Packaging Motors. Glossary. 4.22.1 Packaging in the Double-Stranded DNA Bacteriophages ... 5.15.5 Detection and Quantification of Viral Fusion. 5.15.6 Functional Characterization of Viral Fusion Intermediates: Filling ... 5.15.4 Diverse Viral Proteins Evolved to Mediate Membrane Merger through the Same Principal Mechanism ... 5.14.6 Biophysical Approaches to Understanding Viral Fusion Machines. 5.14.7 Questions Arising from Structural Studies of SNARE ...
https://elsevier.com/books/comprehensive-biophysics/egelman/978-0-12-374920-8

*  'dna chloroplast' Protocols and Video...

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins', 'A Protocol for Functional ... Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair', 'Plasmid-derived DNA Strand ... Identifying Protein-DNA Interactions with Near Base Pair Precision', 'Laboratory Protocol for Genetic Gut Content Analyses of ... and Genomic DNA Mapping of Retroviral Integration Sites', 'Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA- ...
https://jove.com/keyword/dna chloroplast

*  Microbial culturomics: paradigm shift in the human gut microbiome study - Lagier - 2012 - Clinical Microbiology and Infection -...

Metagenomic analysis of respiratory tract DNA viral communities in cystic fibrosis and non-cystic fibrosis individuals. PLoS ...
onlinelibrary.wiley.com/doi/10.1111/1469-0691.12023/references?globalMessage=0

*  Human cytomegalovirus glycoprotein B genotype correlates with different symptoms of infected infants.

0/DNA, Viral; 0/Viral Envelope Proteins; 0/glycoprotein B, Simplexvirus From MEDLINE®/PubMed®, a database of the U.S. National ... DNA Fingerprinting. DNA, Viral / genetics, isolation & purification. Genotype. Humans. Infant. Infant, Newborn. Jaundice / ...
biomedsearch.com/nih/Human-cytomegalovirus-glycoprotein-B-genotype/17356299.html

*  DNA Clean-Up Buffers

NucleoTrap Buffers for DNA clean-up spin columns. ... Viral Transduction * Selection Guides * Viral Systems * ...
clontech.com/US/Products/Nucleic_Acid_Purification/Accessories/Buffers_and_Enzymes/DNA_Clean-Up?sitex=10020:22372:US&PROD=sqFQ121JCqKKoivj5WBi_nix:S&PROD_pses=ZGC487EBFB2F906FB7E118CE951380B235928F9D77FFAEFABAD84A1AEF8951C90B3A96A406F1954B59496D028746E62EB1DFC11F2ECC9EA160

No data available that match "DNA, Viral"



(1/26780) Marker effects on reversion of T4rII mutants.

The frequencies of 2-aminopurine- and 5-bromouracil-induced A:T leads to G:C transitions were compared at nonsense sites throughout the rII region of bacteriophage T4. These frequencies are influenced both by adjacent base pairs within the nonsense codons and by extracodonic factors. Following 2AP treatment, they are high in amber (UAG) and lower in opal (UGA) codons than in allelic ochre (UAA) codons. In general, 5BU-induced transitions are more frequent in both amber and opal codons than in the allelic ochre codons. 2AP- and 5BU-induced transition frequencies in the first and third positions of opal codons are correlated with those in the corresponding positions of the allelic ochre codons. Similarly, the frequencies of 2AP-induced transition in the first and second positions of amber codons and their ochre alleles are correlated. However, there is little correlation between the frequencies of 5BU-induced transitions in the first and second positions of allelic amber and ochre codons.  (+info)

(2/26780) Four dimers of lambda repressor bound to two suitably spaced pairs of lambda operators form octamers and DNA loops over large distances.

Transcription factors that are bound specifically to DNA often interact with each other over thousands of base pairs [1] [2]. Large DNA loops resulting from such interactions have been observed in Escherichia coli with the transcription factors deoR [3] and NtrC [4], but such interactions are not, as yet, well understood. We propose that unique protein complexes, that are not present in solution, may form specifically on DNA. Their uniqueness would make it possible for them to interact tightly and specifically with each other. We used the repressor and operators of coliphage lambda to construct a model system in which to test our proposition. lambda repressor is a dimer at physiological concentrations, but forms tetramers and octamers at a hundredfold higher concentration. We predict that two lambda repressor dimers form a tetramer in vitro when bound to two lambda operators spaced 24 bp apart and that two such tetramers interact to form an octamer. We examined, in vitro, relaxed circular plasmid DNA in which such operator pairs were separated by 2,850 bp and 2,470 bp. Of these molecules, 29% formed loops as seen by electron microscopy (EM). The loop increased the tightness of binding of lambda repressor to lambda operator. Consequently, repression of the lambda PR promoter in vivo was increased fourfold by the presence of a second pair of lambda operators, separated by a distance of 3,600 bp.  (+info)

(3/26780) Human topoisomerase I promotes initiation of simian virus 40 DNA replication in vitro.

Addition of purified human topoisomerase I (topo I) to simian virus 40 T antigen-driven in vitro DNA replication reactions performed with topo I-deficient extracts results in a greater than 10-fold stimulation of completed molecules as well as a more than 3-fold enhancement of overall DNA replication. To further characterize this stimulation, we first demonstrate that bovine topo I but not Escherichia coli topo I can also enhance DNA replication. By using several human topo I mutants, we show that a catalytically active form of topo I is required. To delineate whether topo I influences the initiation or the elongation step of replication, we performed delayed pulse, pulse-chase, and delayed pulse-chase experiments. The results illustrate that topo I cannot promote the completion of partially replicated molecules but is needed from the beginning of the reaction to initiate replication. Competitive inhibition experiments with the topo I binding T antigen fragment 1-246T and a catalytically inactive topo I mutant suggest that part of topo I's stimulation of replication is mediated through a direct interaction with T antigen. Collectively, our data indicate that topo I enhances the synthesis of fully replicated DNA molecules by forming essential interactions with T antigen and stimulating initiation.  (+info)

(4/26780) Induction of AT-specific DNA-interstrand crosslinks by bizelesin in genomic and simian virus 40 DNA.

Bizelesin is a bifunctional AT-specific DNA alkylating drug. Our study characterized the ability of bizelesin to induce interstrand crosslinks, a potential lethal lesion. In genomic DNA of BSC-1 cells, bizelesin formed from approx. 0.3 to 6.03+/-0.85 interstrand crosslinks per 106 base pairs, at 5-100 nM drug concentration, respectively, comparable to the number of total adducts previously determined in the same system (J.M. Woynarowski, M.M. McHugh, L.S. Gawron, T.A. Beerman, Biochemistry 34 (1995) 13042-13050). Bizelesin did not induce DNA-protein crosslinks or strand breaks. A model defined target, intracellular simian virus 40 (SV40) DNA, was employed to map at the nucleotide level sites of bizelesin adducts, including potential interstrand crosslinks. Preferential adduct formation was observed at AT tracts which are abundant in the SV40 matrix associated region and the origin of replication. Many sites, including each occurrence of 5'-T(A/T)4A-3', co-mapped on both DNA strands suggesting interstrand crosslinks, although monoadducts were also formed. Bizelesin adducts in naked SV40 DNA were found at similar sites. The localization of bizelesin-induced crosslinks in AT-rich tracts of replication-related regions is consistent with the potent anti-replicative properties of bizelesin. Given the apparent lack of other types of lesions in genomic DNA, interstrand crosslinks localized in AT-rich tracts, and to some extent perhaps also monoadducts, are likely to be lethal effects of bizelesin.  (+info)

(5/26780) Hybrid capture II, a new sensitive test for human papillomavirus detection. Comparison with hybrid capture I and PCR results in cervical lesions.

AIM: To test a new assay for the detection of human papillomavirus (HPV) DNA, hybrid capture II (HC II), compared with the previous commercialized hybrid capture I (HC I) and polymerase chain reaction (PCR) results on cervical scrapes from fresh cone excision biopsy samples. METHODS: The three methods were used on cervical scrapes from 42 fresh cone excision biopsy samples. There were nine metaplastic and inflammatory lesions, five low grade lesions, and 28 high grade lesions. PCR was performed using the general primers GP5+/GP6+. The viral load of high risk HPV DNA was estimated by the ratio of relative light units to positive control values in the samples. RESULTS: The sensitivity of HC I for the detection of high grade lesions was 71.4%, while it was 92.8% for HC II and 96.4% for the PCR. Considering only the absence of detectable cervical in situ neoplasia, the specificity was 88.9% for HC I, 66.7% for HC II, and 66.7% for PCR. With HC II, for a ratio of cervical sample to normal control of > 200, the sensitivity for the detection of high grade lesion was only 34.6% with a specificity of 66.7%. CONCLUSIONS: HPV detection with the HC II assay is more sensitive than the previous HC I and represents a more convenient and easier test than PCR for routine use. Nevertheless the viral load estimated with this test cannot be a reliable predictive indicator of high grade lesions.  (+info)

(6/26780) Human papillomavirus DNA in adenosquamous carcinoma of the lung.

AIM: To investigate the presence of human papillomavirus (HPV) DNA in adenosquamous carcinoma of the lung--which is relatively common in Okinawa but not in mainland Japan--and examine its histological features. METHODS: Of 207 cases where primary lung cancers were surgically removed between January 1995 and June 1997 in Okinawa, 23 were adenosquamous carcinoma. HPV was detected by non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR) amplification with primers specific for E6 and E7 regions of the HPV genome. PCR products were analysed by Southern blotting. Immunohistochemical determination of high molecular weight cytokeratin (HMC) and involucrin was also carried out. RESULTS: 18 cases were positive for HPV DNA by PCR and NISH. HPV types 6, 11, 16, and 18 were found. Seven cases were dual positive for different types of HPV. Using NISH, HPV was also found in the squamous cell components and in neighbouring enlarged adenocarcinoma cells. The HMC and involucrin were demonstrated immunohistochemically in the same areas. CONCLUSIONS: HPV DNA was found in a high proportion (78.3%) of adenosquamous carcinomas in Okinawa, a region where HPV has previously been shown to be prevalent in squamous cell carcinoma of the lung. The adenocarcinoma cells adjacent to the squamous cell carcinoma component were enlarged and positive for HPV, HMC, and involucrin. This is thought to indicate the transition from adenocarcinoma to squamous cell carcinoma.  (+info)

(7/26780) A review of statistical methods for estimating the risk of vertical human immunodeficiency virus transmission.

BACKGROUND: Estimation of the risk of vertical transmission of human immunodeficiency virus (HIV) has been complicated by the lack of a reliable diagnostic test for paediatric HIV infection. METHODS: A literature search was conducted to identify all statistical methods that have been used to estimate HIV vertical transmission risk. Although the focus of this article is the analysis of birth cohort studies, ad hoc studies are also reviewed. CONCLUSIONS: The standard method for estimating HIV vertical transmission risk is biased and inefficient. Various alternative analytical approaches have been proposed but all involve simplifying assumptions and some are difficult to implement. However, early diagnosis/exclusion of infection is now possible because of improvements in polymerase chain reaction technology and complex estimation methods should no longer be required. The best way to analyse studies conducted in breastfeeding populations is still unclear and deserves attention in view of the many intervention studies being planned or conducted in developing countries.  (+info)

(8/26780) Novel endotheliotropic herpesviruses fatal for Asian and African elephants.

A highly fatal hemorrhagic disease has been identified in 10 young Asian and African elephants at North American zoos. In the affected animals there was ultrastructural evidence for herpesvirus-like particles in endothelial cells of the heart, liver, and tongue. Consensus primer polymerase chain reaction combined with sequencing yielded molecular evidence that confirmed the presence of two novel but related herpesviruses associated with the disease, one in Asian elephants and another in African elephants. Otherwise healthy African elephants with external herpetic lesions yielded herpesvirus sequences identical to that found in Asian elephants with endothelial disease. This finding suggests that the Asian elephant deaths were caused by cross-species infection with a herpesvirus that is naturally latent in, but normally not lethal to, African elephants. A reciprocal relationship may exist for the African elephant disease.  (+info)



anti-viral


  • Gene ontology and pathway analyses clearly demonstrated that these genes activated by DNA demethylation are involved in the anti-viral response. (aacrjournals.org)
  • DNA demethylation suppresses the proliferation of intestinal tumor organoids by inducing an anti-viral response including activation of interferon-related genes. (aacrjournals.org)

replication


  • In this era, there are medications that can suppress hbv dna replication, i.e. (healthtap.com)
  • Common mechanisms for viral DNA replication ( How DNA replicates is a critical questi. (bio-medicine.org)
  • DNA replication remains difficult to investigate in eukaryotes,where it occurs within the confines of the double-membrane nucleus. (bio-medicine.org)
  • In simian virus 40 (SV40), the large T-antigen protein (T-ag) is responsible for recognizing DNA sequences required to start replication, called the origin of replication. (bio-medicine.org)
  • In a new study published online in the open access journal PLoS Biology, Gretchen Meinke, Andrew Bohm, and colleagues report the crystal structure of the DNA-binding domain of SV40 T-ag on a DNA fragment derived from the viral origin of replication. (bio-medicine.org)
  • To verify the safety and the efficacy of the study treatment, defined as the persistent control of the virus' replication at 48 weeks after the simplification to maraviroc + darunavir with ritonavir in patients with R5 tropism by viral DNA genotyping. (clinicaltrials.gov)
  • Objective: To assess the effect of GM-CSF on viral replication and the potential ameliorative effect of antiretroviral therapy. (lww.com)
  • Viral replication was assessed by p24 expression in the supernatant and aggregates. (lww.com)
  • Conclusions: This study demonstrates that GM-CSF enhances viral replication while addition of stavudine prevents this potentially detrimental process. (lww.com)

genetic


  • To generate swarms of new viral particles, a virus hijacks a cell into producing masses of self-assembling cages that are then loaded with the genetic blueprint for the next infection. (jefferson.edu)
  • RABIES (Viral load) Qualitative RABIES (Viral load) Qualitative Genetic dna test is done using Real Time PCR and sample type is Saliva, Tissue, C.S.F. Conjunctiva swab total time for result reports is 3rd Working Day Email:-36 hours. (dnalabsindia.com)

infections


  • EIGHT PERCENT of our DNA is of viral origin , the remnants of ancient infections. (blogspot.com)

particles


  • Decrease the generation of new viral particles, decreasing injury to the liver and long term complications, such as cirrhosis and liver cancer . (healthtap.com)
  • We have shown that the portal is much more like a sensor that essentially helps measure out an appropriate length of DNA for each capsid particle, ensuring faithful production of new viral particles. (jefferson.edu)

viruses


  • The scientists found bits of viral DNA embedded in our genome are actually regulating genes that are integral components of our innate immune system - which is the first line of defense against pathogens, including viruses. (blogspot.com)
  • But the picture of how that DNA is loaded into those viral cages, or capsids, was blurry, especially for two of the most common types of DNA virus on earth, bacterial viruses and human herpesvirus. (jefferson.edu)

dementia


  • Importantly, it has been observed that these cognitive disorders also correlate with higher levels of viral RNA in the cerebrospinal fluid, indicating that progression of HIV-related brain damage and cognitive impairment and dementia is associated with increased central nervous system viral burden [ 16,17 ]. (lww.com)

sequences


  • An intriguing wrinkle to this study is the previously reported presence of sequences highly homologous to MMTV Env protein in the DNA of as many as 40% of human breast tumors, although a human homologue of MMTV has yet to be found. (bio-medicine.org)

purification


  • The kit provides reagents for the purification of viral DNA/RNA from up to 200 µl sample volume. (clontech.com)

protein


  • The structure shows that although T-ag and its functionally analogous protein, papilloma virus E1, share no detectable sequence homology in this region, the two domains bind the DNA in similar ways. (bio-medicine.org)
  • In addition to describing the molecular details of the DNA-protein interactions and the alterations in protein structure induced by DNA binding, we present a model describing the subsequent assembly events. (bio-medicine.org)
  • A small sequence of DNA in the envelope (Env) protein of a mouse breas. (bio-medicine.org)
  • A small sequence of DNA in the envelope (Env) protein of a mouse breast tumor virus (called MMTV) can transform breast cells into cancer cells, according to a study by Katz et al. (bio-medicine.org)
  • Thomas Jefferson University researchers pieced together the three-dimensional atomic structure of a doughnut-shaped protein that acts like a door or 'portal' for the DNA to get in and out of the capsid, and have now discovered that this protein begins to transform its structure when it comes into contact with DNA. (jefferson.edu)
  • Researchers thought that the portal protein acts as an inert passageway for DNA," says senior author Gino Cingolani, Ph.D., a Professor in the Department of Biochemistry and Molecular Biology at Thomas Jefferson University and researcher at the Sidney Kimmel Cancer Center . (jefferson.edu)
  • Paradoxically, though, the protein wasn't very good at binding to DNA, which should have been an essential ability, the researchers thought. (jefferson.edu)
  • Unlike the mature final-stage protein that was profoundly symmetric, this immature conformation of portal protein is surprisingly asymmetric and has the ability to bind strongly to both the motor and the DNA itself. (jefferson.edu)
  • We think that DNA binds to the immature portal protein and wraps around it like a python, as it enters the viral capsid with the help of the motor protein. (jefferson.edu)
  • This DNA stranglehold causes the portal protein to begin to transform into its final symmetric state that because of its weak binding will ultimately release both the DNA and the motor, cutting off the DNA-loading at an appropriate length," says Dr. Cingolani. (jefferson.edu)
  • It took us 18 years to understand that the portal protein functions by existing in two states that turn the viral DNA packaging on and off by changing its structure. (jefferson.edu)
  • Cingolani, G. "Portal protein functions akin to a DNA-sensor that couples genome-packaging to icosahedral capsid maturation. (jefferson.edu)

load


  • Hbv DNA viral load result: 451.35 iu/ml (1895.67 copies/ml) HBsAG 4.441 and antihbs 0.10. (healthtap.com)
  • Viral load of 2255? (healthtap.com)
  • What is the does my hepatitis b viral load of 25997 mean? (healthtap.com)
  • Can a person get completely cured of hepatitis b once his viral load is constantly negative and his HBsAG and hbeag is negative? (healthtap.com)
  • Hepatitis b. new diagnosis of hep b, dangerous livers #s. after follow up in 3 wks liver #s normal, hep b viral load, 12000 iu. (healthtap.com)
  • I am suffer from hepatitis b my viral load is reduced from 5000 to 3 point at last year 2012. (healthtap.com)
  • I am not sure what "3 point" means, but this is a low viral load. (healthtap.com)
  • The viral load and liver enzymes need to be measured about twice a year so stay in touch with your health provider. (healthtap.com)
  • My hbv viral load is 40 iu/ml, hbv dna load is 233cp/ml, what does it mean. (healthtap.com)
  • in addition, therapeutic GM-CSF elevates plasma viral load. (lww.com)
  • First year it was ok but last 2 tests my viral load is above 10.000. (medhelp.org)
  • Yes the sample will be collected from home for this RABIES (Viral load) Qualitative for free of cost from Delhi, Mumbai, Kolkata Chennai, Bnaglaore, Hyderabad, ahmedabad and pune. (dnalabsindia.com)
  • You can make the payment online and through debit card, credit card & net banking for RABIES (Viral load) Qualitative. (dnalabsindia.com)
  • all RABIES (Viral load) Qualitative samples are processed twice in order to double-check the result. (dnalabsindia.com)
  • It is very easy to order a RABIES (Viral load) Qualitative. (dnalabsindia.com)
  • HIV and HBV DNA viral load will be tested every 12 weeks. (clinicaltrials.gov)

serum


  • NucleoMag 96 Virus is designed for rapid manual and automated small-scale preparation of viral RNA/DNA from cell-free body fluids such as serum or plasma. (clontech.com)

therapeutic


  • Because some herpesviruses infect and lay dormant in human cells until they reawaken by stress, developing a therapy that could interfere with viral production at different levels could prove a useful therapeutic strategy. (jefferson.edu)
  • These findings suggest that treatment of colon cancers with DNA methylation inhibitors such as 5-Aza-CdR may be an effective therapeutic approach inhibiting cancer stem cells by immune response. (aacrjournals.org)

carrier


  • You are a hepatitis b carrier - with high levels of hepatitis b surface antigen (hbsag) as well as hbv dna positive. (healthtap.com)

diseases


  • The finding solves a longstanding puzzle in the field, and reveals a potential drug target for one of the most common human viral pathogens, herpesviruses, which is responsible for diseases such as chicken pox, mononucleosis, lymphomas and Kaposi sarcoma. (jefferson.edu)

genes


  • This study puts the spotlight on a potential new mechanism for virus-induced breast cell transformation, which has largely been attributed to the integration of viral DNA into locations that trigger the expression of host cancer-causing genes. (bio-medicine.org)
  • It is believed that the anti-tumor effect of DNA methylation inhibitors is mediated by re-activation of epigenetically silenced tumor suppressor genes in cancer cells. (aacrjournals.org)
  • However, recent studies have proposed that the major anti-tumor effect of DNA methylation inhibitors is induction of interferon-related genes via dsRNAs-containing endogenous retroviruses. (aacrjournals.org)
  • Microarray analyses of the tumor organoids after 5-Aza-CdR treatment and Dnmt1 knockdown revealed that interferon-related genes including interferon regulatory factor 7 (Irf7) were activated by DNA demethylation. (aacrjournals.org)

proteins


  • In both cases, DNA binding is thought to initiate assembly of a complex of the full-length proteins on DNA. (bio-medicine.org)
  • And that it must also adopt other conformations earlier in viral assembly that have the capacity to bind both DNA and other motor proteins, or terminases. (jefferson.edu)

human


  • http://ottersandsciencenews.blogspot.ca/2016/03/how-viral-dna-in-our-human-genome.html - Thank you for visiting my blog. (blogspot.com)
  • 2) DNA specialists have DELIBERATELY disregarded this viral DNA in the human genome. (blogspot.com)
  • Due to Dr Frank Ryan's work and that of other similarly-minded scientists, the truth about the positive role played by the symbiosis between virus and vertebrate DNA and their role in human evolution, is finally starting to become mainstream. (blogspot.com)
  • There has been evidence for some time that some viral DNA living in symbiosis with vertebrate DNA in the human genome plays a protective role in our immune system. (blogspot.com)

study


  • Study by scientists at the University of Utah School of Medicine show that evolution has repurposed some of these viral remains into weapons against its own kind. (blogspot.com)

cells


  • The DNA sequence in question is usually found in immune cells and help. (bio-medicine.org)
  • The ability of this motif to transform cells single-handedly suggests that viral infection may be an important and previously unrecognized trigger for breast cancer. (bio-medicine.org)

Virus


  • The viral hemorrhagic septicemia virus (VHSV) is one of the most serious fish pathogens. (biomedcentral.com)
  • We have previously demonstrated that a DNA plasmid (pcDNA), containing a cytomegalovirus (CMV) promoter and the viral hemorrhagic septicemia virus (VHSV) genotype IVb glycoprotein (G) gene insert (designated pVHSivb-G) confers moderate protection in muskellunge ( Esox masquinongy ), a highly susceptible species upon challenge. (biomedcentral.com)

immune


  • They are an integral part of our DNA and immune system. (blogspot.com)
  • Within mammalian genomes are reservoirs of viral DNA that have fueled innovation of the innate immune system. (blogspot.com)

results


  • They ignore it because they can't explain it, thus distorting their DNA-related results. (blogspot.com)

found


  • Dna are the coding molecules found in all living things on earth which contain the instructions on how to build living things. (healthtap.com)

time


  • In order to achieve optimal protection, we investigated a number of factors including the incubation time [i.e. the number of degree days (° days)] before challenge, and viral challenge dose and route. (biomedcentral.com)

treatment


  • To investigate the effect of DNA demethylation on tumor organoids, we have established intestinal tumor organoids from tumors of ApcMin/+ (Min) mice and subjected them to 5-aza-2'-deoxycytidine (5-Aza-CdR) treatment and Dnmt1 knockdown. (aacrjournals.org)
  • DNA demethylation induced by 5-Aza-CdR treatment and Dnmt1 knockdown significantly reduced the cell proliferation of the tumor organoids. (aacrjournals.org)

life


  • How DNA replicates is a critical question for understanding life. (bio-medicine.org)

administration


  • An increase in the period lapsed between vaccination and challenge to 1880° days resulted in 95% relative percent protection (RPS) in muskellunge following a single administration of the pVHSivb-G plasmid and viral challenge. (biomedcentral.com)

completely


  • It's a completely novel mechanism for sensing DNA. (jefferson.edu)

host