DNA, Satellite
Begomovirus
Satellite Cells, Skeletal Muscle
RNA, Satellite
Satellite Communications
Satellite Viruses
Satellite Cells, Perineuronal
PAX7 Transcription Factor
Satellite Imagery
Hospitals, Satellite
Spacecraft
Cucumber Mosaic Virus Satellite
Muscle Development
MyoD Protein
Muscle, Skeletal
Centromere
Tobacco mosaic satellite virus
Muscle Fibers, Skeletal
Remote Sensing Technology
Genome, Human
Antibodies, Immobilized
Genome
DNA
Human Genome Project
Tumor necrosis factor a microsatellite polymorphism is associated with rheumatoid arthritis severity through an interaction with the HLA-DRB1 shared epitope. (1/1056)
OBJECTIVE: To determine whether tumor necrosis factor microsatellite a (TNFa) polymorphism is associated with severity of rheumatoid arthritis (RA), and to examine the evidence for interaction between TNFa and the HLA-DRB1 shared epitope (SE). METHODS: One hundred seventy-one community-based white female RA patients were genotyped for both TNFa and HLA-DRB1 alleles. We performed pairwise association analyses, stratified analyses, and multivariate logistic regressions to determine whether TNFa was associated with 4 measures of RA severity, and whether there was significant interaction between TNFa and the HLA-DRB1 SE. RESULTS: Simple pairwise analyses did not reveal significant association between TNFa polymorphism and RA severity. However, when the data were stratified by the presence versus absence of the SE, striking associations were observed between TNFa allele 11 (TNFa11) and RA severity. These analyses also demonstrated significant interaction between TNFa11 and the SE (P = 0.07-0.005), and this was confirmed in our multivariate regressions. Specifically, the most severe outcomes were observed among individuals who had inherited both TNFa11 and the SE (61-71% had severe RA based on 1 of the 4 outcomes). In contrast, individuals who had inherited TNFa11 in the absence of the SE had the best outcomes (8-21% with severe RA). The odds ratios comparing these 2 groups ranged from 8.8 to 22.7 for the 4 severity measures. The differential effect of TNFa11 according to the presence versus absence of the SE (and vice versa) illustrated their interaction with respect to RA severity. CONCLUSION: The data suggest that TNFa is associated with RA severity through an interaction with the HLA-DRB1 SE. (+info)Characterization and chromosomal distribution of satellite DNA sequences of the water buffalo (Bubalus bubalis). (2/1056)
Satellite DNA sequences were isolated from the water buffalo (Bubalus bubalis) after digestion with two restriction endonucleases, BamHI and StuI. These satellite DNAs of the water buffalo were classified into two types by sequence analysis: one had an approximately 1,400 bp tandem repeat unit with 79% similarity to the bovine satellite I DNA; the other had an approximately 700 bp tandem repeat unit with 81% similarity to the bovine satellite II DNA. The chromosomal distribution of the satellite DNAs were examined in the river-type and the swamp-type buffaloes with direct R-banding fluorescence in situ hybridization. Both the buffalo satellite DNAs were localized to the centromeric regions of all chromosomes in the two types of buffaloes. The hybridization signals with the buffalo satellite I DNA on the acrocentric autosomes and X chromosome were much stronger than that on the biarmed autosomes and Y chromosome, which corresponded to the distribution of C-band-positive centromeric heterochromatin. This centromere-specific satellite DNA also existed in the interstitial region of the long arm of chromosome 1 of the swamp-type buffalo, which was the junction of the telomere-centromere tandem fusion that divided the karyotype in the two types of buffaloes. The intensity of the hybridization signals with buffalo satellite II DNA was almost the same over all the chromosomes, including the Y chromosome, and no additional hybridization signal was found in noncentromeric sites. (+info)QT interval is linked to 2 long-QT syndrome loci in normal subjects. (3/1056)
BACKGROUND: The rate-corrected QT interval (QTc) is heritable, and the discovery of quantitative trait loci that influence the QTc would be an important step in identifying the genes responsible for life-threatening arrhythmias in the general population. We studied 66 pairs of unselected normal dizygotic (DZ) twin subjects and their parents in a sib-pair analysis. We tested for linkage of gene loci harboring genes known to cause the long-QT syndrome (LQT) to the quantitative trait QTc. METHODS AND RESULTS: We found genetic variance on QRS duration, QRS axis, T-wave axis, and QTc. Women had a longer QTc than men. Microsatellite markers were tested in the vicinity of the gene loci for the 5 known LQT genes. We found significant linkage of QTc with the loci for LQT1 on chromosome 11 and LQT4 on chromosome 4 but not to LQT2, LQT3, or LQT5. We also found linkage of the QRS axis with LQT2 and LQT3. CONCLUSIONS: We suggest that these quantitative trait loci may represent the presence of variations in LQT genes that could be important to the risk for rhythm disturbances in the general population. (+info)Loss of heterozygosity and microsatellite instability at the MLL locus are common in childhood acute leukemia, but not in infant acute leukemia. (4/1056)
Rearrangements involving the MLL gene at chromosome 11q23 are associated with leukemia and are present in up to 70% of infant leukemias. Loss of heterozygosity (LOH) has been shown for anonymous polymorphic markers at 11q23 in adult leukemias. To study LOH at the MLL locus, we have identified two new polymorphic microsatellite markers: a GAA repeat (mllGAAn) in intron 6 of the MLL gene and a GA (mllGAn) repeat in the 5' flanking region of the gene, approximately 2 kb upstream of the translation initiation codon. The heterozygosity index of mllGAAn is 0.54, which renders it useful for analyzing LOH. We screened two groups of leukemia patients to study LOH at the mllGAAn marker. Group A (n = 18) was selected on the basis of presentation before 18 months. Cytogenetic and reverse transcription-polymerase chain reaction analysis showed that 9 of these 18 children had translocations involving MLL. No LOH was observed. Group B (n = 36) were randomly selected children who had presented with leukemia between 1993 and 1994. Cytogenetic analysis of this group showed a variety of different chromosomal abnormalities. LOH was shown in 9 of 20 individuals (45%) who were informative. Microsatellite instability (MSI) was demonstrated in 1 of 18 individuals in group A and 5 of 36 individuals (13.9%) in group B. MSI and LOH were observed simultaneously in three individuals. Loss of an allele was confirmed in one individual by fluorescence in situ hybridization. Individuals with MSI or LOH at mllGAAn were selected for analysis at anonymous polymorphic markers D11S1364 and D11S1356, which flank the MLL gene. No LOH or MSI was observed at these markers in those individuals who were informative. These results show that LOH at the MLL gene locus is a common event during leukemogenesis. Furthermore, the presence of MSI at this locus suggests that the region is a hotspot for genetic instability. (+info)DDP1, a single-stranded nucleic acid-binding protein of Drosophila, associates with pericentric heterochromatin and is functionally homologous to the yeast Scp160p, which is involved in the control of cell ploidy. (5/1056)
The centromeric dodeca-satellite of Drosophila forms altered DNA structures in vitro in which its purine-rich strand (G-strand) forms stable fold-back structures, while the complementary C-strand remains unstructured. In this paper, the purification and characterization of DDP1, a single-stranded DNA-binding protein of high molecular mass (160 kDa) that specifically binds the unstructured dodeca-satellite C-strand, is presented. In polytene chromosomes, DDP1 is found located at the chromocentre associated with the pericentric heterochromatin but its distribution is not constrained to the dodeca-satellite sequences. DDP1 also localizes to heterochromatin in interphase nuclei of larval neuroblasts. During embryo development, DDP1 becomes nuclear after cellularization, when heterochromatin is fully organized, being also associated with the condensed mitotic chromosomes. In addition to its localization at the chromocentre, in polytene chromosomes, DDP1 is also detected at several sites in the euchromatic arms co-localizing with the heterochromatin protein HP1. DDP1 is a multi-KH domain protein homologous to the yeast Scp160 protein that is involved in the control of cell ploidy. Expression of DDP1 complements a Deltascp160 deletion in yeast. These results are discussed in view of the possible contribution of DNA structure to the structural organization of pericentric heterochromatin. (+info)A gene adjacent to satellite DNA in Drosophila melanogaster. (6/1056)
Several copies of a sequence adjacent to 1.688 g/cm3 satellite DNA in the Drosophila melanogaster genome have been isolated by molecular cloning. This sequence, called the Dm142 gene, is homologous to a 1.6-kilobase RNA found in both D. melanogaster embryos and tissue culture cells. One cloned DNA segment includes two copies of the Dm142 gene and 1.688 g/cm3 satellite DNA sequences, which are located between and flanking both gene copies. The Dm142 gene is repeated many times in the D. melanogaster genome, and some copies are not flanked by 1,688 g/cm3 satellite DNA. (+info)Role of multicolor fluorescence in situ hybridization (FISH) in simultaneous detection of probe sets for chromosome 18, X and Y in uncultured amniotic fluid cells. (7/1056)
Major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. The purpose of this study was to evaluate the role of multicolor fluorescence in situ hybridization in simultaneous detection of probe sets for chromosome 18, X, and Y in uncultured amniotic fluid cells as a safer alternative method for aneuploidy detection prenatally. Fifty amniotic fluid samples were analyzed by FISH and standard cytogenetics. Mean time to obtain results was three days for fluorescence in situ hybridization and 20 days for karyotype. Fluorescence in situ hybridization was informative in 43 samples (86%), and within this group, two aneuploidies were correctly identified. This evaluation demonstrates that FISH with X, Y, and 18 alpha satellite DNA probes could accurately and rapidly detect aneuploidies involving these chromosomes and could be used in any prenatal clinical laboratory. (+info)Regulated formation of extrachromosomal circular DNA molecules during development in Xenopus laevis. (8/1056)
Extrachromosomal circular DNA molecules of chromosomal origin have been detected in many organisms and are thought to reflect genomic plasticity in eukaryotic cells. Here we report a developmentally regulated formation of extrachromosomal circular DNA that occurs de novo in preblastula Xenopus embryos. This specific DNA population is not detected in the male or female germ cells and is dramatically reduced in later developmental stages and in adult tissues. The activity responsible for the de novo production of extrachromosomal circles is maternally inherited, is stored in the unfertilized egg, and requires genomic DNA as a template. The formation of circular molecules does not require genomic DNA replication but both processes can occur simultaneously in the early development. The production of extrachromosomal circular DNA does not proceed at random since multimers of the tandemly repeated sequence satellite 1 were over-represented in the circle population, while other sequences (such as ribosomal DNA and JCC31 repeated sequence) were not detected. This phenomenon reveals an unexpected plasticity of the embryonic genome which is restricted to the early developmental stage. (+info)Satellite DNA is a type of DNA sequence that is repeated in a tandem arrangement in the genome. These repeats are usually relatively short, ranging from 2 to 10 base pairs, and are often present in thousands to millions of copies arranged in head-to-tail fashion. Satellite DNA can be found in centromeric and pericentromeric regions of chromosomes, as well as at telomeres and other heterochromatic regions of the genome.
Due to their repetitive nature, satellite DNAs are often excluded from the main part of the genome during DNA sequencing projects, and therefore have been referred to as "satellite" DNA. However, recent studies suggest that satellite DNA may play important roles in chromosome structure, function, and evolution.
It's worth noting that not all repetitive DNA sequences are considered satellite DNA. For example, microsatellites and minisatellites are also repetitive DNA sequences, but they have different repeat lengths and arrangements than satellite DNA.
Begomovirus is a genus of viruses in the family *Geminiviridae* that infect plants. These viruses are transmitted by insects, specifically whiteflies, and have circular, single-stranded DNA genomes. Begomoviruses cause various diseases in economically important crops, such as tomatoes, beans, cassava, and cotton, leading to significant yield losses worldwide. The name "Begomovirus" is derived from the type species *Bean golden mosaic virus*, which was isolated from beans in Mexico.
A plant disease is a disorder that affects the normal growth and development of plants, caused by pathogenic organisms such as bacteria, viruses, fungi, parasites, or nematodes, as well as environmental factors like nutrient deficiencies, extreme temperatures, or physical damage. These diseases can cause various symptoms, including discoloration, wilting, stunted growth, necrosis, and reduced yield or productivity, which can have significant economic and ecological impacts.
Satellite cells in skeletal muscle are undifferentiated stem cells that are crucial for postnatal growth, maintenance, and repair of skeletal muscle. They are located between the basal lamina and plasma membrane of myofibers. In response to muscle damage or injury, satellite cells become activated, proliferate, differentiate into myoblasts, fuse with existing muscle fibers, and contribute to muscle regeneration. Satellite cells also play a role in maintaining muscle homeostasis by fusing with mature muscle fibers to replace damaged proteins and organelles. They are essential for the adaptation of skeletal muscle to various stimuli such as exercise or mechanical load.
A satellite RNA is a type of non-coding RNA that does not encode proteins but instead plays a role in the regulation of gene expression. It is so named because it can exist as a separate, smaller molecule that "satellites" around a larger RNA molecule called the helper RNA. Satellite RNAs are often associated with viruses and can affect their replication and packaging. They can also be found in some eukaryotic cells, where they may play a role in regulating the expression of certain genes or in the development of diseases such as cancer.
I'm sorry for any confusion, but "Satellite Communications" is not a term typically used in medical definitions. Satellite communications refers to the use of artificial satellites in space to provide communication links between various points on Earth. This technology is widely used in many fields including telecommunications, broadcasting, military, and transportation, but it is not a medical concept. If you have any questions related to medical terminology or concepts, I'd be happy to help with those instead!
"Satellite viruses" are a type of viruses that require the presence of another virus, known as a "helper virus," to complete their replication cycle. They lack certain genes that are essential for replication and therefore depend on the helper virus to provide these functions. Satellite viruses can either be satellite RNA or satellite DNA viruses, and they can affect plants, animals, and bacteria.
Satellite viruses can influence the severity of the disease caused by the helper virus, either increasing or decreasing it. They can also interfere with the replication of the helper virus and affect its transmission. The relationship between satellite viruses and their helper viruses is complex and can vary depending on the specific viruses involved.
It's important to note that the term "satellite virus" is not used consistently in the scientific literature, and some researchers may use it to refer to other types of dependent or defective viruses. Therefore, it's always a good idea to consult the original research when interpreting the use of this term.
Perineuronal satellite cells are a type of glial cell that surround and enwrap the neurons in the peripheral nervous system. They are called "satellite" cells because they appear to be clustered around the neuron like satellites orbiting a planet. These cells play important roles in maintaining the homeostasis of the neural microenvironment, providing structural support, and contributing to the regulation of neurotransmitter synthesis, uptake, and metabolism. They also have the ability to proliferate and differentiate into other cell types under certain conditions, making them a potential source for cell-based therapies in nerve injuries and neurodegenerative diseases.
PAX7 is a transcription factor that belongs to the PAX (paired box) family of proteins, which are characterized by the presence of a paired domain that binds to DNA. Specifically, PAX7 contains two DNA-binding domains: a paired domain and a homeodomain.
PAX7 is primarily expressed in satellite cells, which are muscle stem cells responsible for postnatal muscle growth, maintenance, and regeneration. PAX7 plays a critical role in the self-renewal and survival of satellite cells, and its expression is required for their activation and differentiation into mature muscle fibers.
As a transcription factor, PAX7 binds to specific DNA sequences in the regulatory regions of target genes and regulates their expression. This regulation can either activate or repress gene transcription, depending on the context and other factors that interact with PAX7.
PAX7 has been implicated in various muscle-related diseases, including muscular dystrophies and muscle wasting disorders. Its expression is often downregulated in these conditions, leading to a decrease in satellite cell function and muscle regeneration capacity. Therefore, understanding the role of PAX7 in muscle biology and disease has important implications for developing new therapies for muscle-related diseases.
I'm sorry for any confusion, but "satellite imagery" is not a medical term. It refers to photographs or images taken from satellites orbiting the Earth. These images are used in various fields, including meteorology, geology, agriculture, and military surveillance, among others. They can provide information about large-scale phenomena such as weather patterns, natural disasters, and environmental changes. However, they are not typically used in medical contexts.
A satellite hospital is a healthcare facility that is physically separate but operationally connected to a larger medical center or "main" hospital. These facilities are often located in outlying areas or suburbs and provide a range of medical services, including emergency care, diagnostic tests, and outpatient procedures. They are designed to bring healthcare services closer to where patients live and work, making it more convenient for them to access care.
Satellite hospitals are typically smaller than main hospitals and may not offer the same level of specialized care or resources. However, they are often equipped with advanced medical technology and staffed by qualified healthcare professionals who can provide high-quality medical care. In some cases, satellite hospitals may also serve as training sites for medical students and residents.
One of the benefits of satellite hospitals is that they can help to reduce overcrowding in main hospitals and improve access to care for patients who live in rural or underserved areas. They can also provide a cost-effective alternative to building a new main hospital in an area where demand for healthcare services is growing. Overall, satellite hospitals play an important role in expanding access to high-quality healthcare services and improving health outcomes for patients.
I'm sorry for any confusion, but "spacecraft" is not a term typically used in medical definitions. A spacecraft is a vehicle or machine designed to fly in outer space. It may be used to transport humans or cargo to and from space stations, conduct scientific research, or explore other celestial bodies such as the moon, planets, and asteroids. If you have any questions related to medical terminology, I'd be happy to help!
Cucumber mosaic virus (CMV) is a type of plant virus that can cause symptoms such as mosaic patterns on leaves, stunted growth, and reduced yield in various host plants. A satellite of CMV refers to a small, independent RNA molecule that can associate with the viral genome and affect its replication and symptom expression.
The satellite RNA of CMV is known as Satellite Cucumber Mosaic Virus (SCMV). It is a subviral agent that depends on the helper virus (CMV) for its replication, encapsidation, and movement within the host plant. SCMV can modulate the symptoms caused by CMV in infected plants, either attenuating or exacerbating them depending on the strain of SCMV and the host plant.
SCMV is a single-stranded RNA molecule that encodes a single protein, which functions as a coat protein for its own encapsidation. It can also affect the accumulation and symptom expression of CMV, making it an important factor to consider in the study of CMV epidemiology and pathogenesis.
Muscle development, also known as muscle hypertrophy, refers to the increase in size and mass of the muscles through a process called myofiber growth. This is primarily achieved through resistance or strength training exercises that cause micro-tears in the muscle fibers, leading to an inflammatory response and the release of hormones that promote muscle growth. As the muscles repair themselves, they become larger and stronger than before. Proper nutrition, including adequate protein intake, and rest are also essential components of muscle development.
It is important to note that while muscle development can lead to an increase in strength and muscular endurance, it does not necessarily result in improved athletic performance or overall fitness. A well-rounded exercise program that includes cardiovascular activity, flexibility training, and resistance exercises is recommended for optimal health and fitness outcomes.
Regeneration in a medical context refers to the process of renewal, restoration, and growth that replaces damaged or missing cells, tissues, organs, or even whole limbs in some organisms. This complex biological process involves various cellular and molecular mechanisms, such as cell proliferation, differentiation, and migration, which work together to restore the structural and functional integrity of the affected area.
In human medicine, regeneration has attracted significant interest due to its potential therapeutic applications in treating various conditions, including degenerative diseases, trauma, and congenital disorders. Researchers are actively studying the underlying mechanisms of regeneration in various model organisms to develop novel strategies for promoting tissue repair and regeneration in humans.
Examples of regeneration in human medicine include liver regeneration after partial hepatectomy, where the remaining liver lobes can grow back to their original size within weeks, and skin wound healing, where keratinocytes migrate and proliferate to close the wound and restore the epidermal layer. However, the regenerative capacity of humans is limited compared to some other organisms, such as planarians and axolotls, which can regenerate entire body parts or even their central nervous system.
MyoD protein is a member of the family of muscle regulatory factors (MRFs) that play crucial roles in the development and regulation of skeletal muscle. MyoD is a transcription factor, which means it binds to specific DNA sequences and helps control the transcription of nearby genes into messenger RNA (mRNA).
MyoD protein is encoded by the MYOD1 gene and is primarily expressed in skeletal muscle cells, where it functions as a master regulator of muscle differentiation. During myogenesis, MyoD is activated and initiates the expression of various genes involved in muscle-specific functions, such as contractile proteins and ion channels.
MyoD protein can also induce cell cycle arrest and promote the differentiation of non-muscle cells into muscle cells, a process known as transdifferentiation. This property has been explored in regenerative medicine for potential therapeutic applications.
In summary, MyoD protein is a key regulator of skeletal muscle development, differentiation, and maintenance, and it plays essential roles in the regulation of gene expression during myogenesis.
Skeletal muscle, also known as striated or voluntary muscle, is a type of muscle that is attached to bones by tendons or aponeuroses and functions to produce movements and support the posture of the body. It is composed of long, multinucleated fibers that are arranged in parallel bundles and are characterized by alternating light and dark bands, giving them a striped appearance under a microscope. Skeletal muscle is under voluntary control, meaning that it is consciously activated through signals from the nervous system. It is responsible for activities such as walking, running, jumping, and lifting objects.
A centromere is a specialized region found on chromosomes that plays a crucial role in the separation of replicated chromosomes during cell division. It is the point where the sister chromatids (the two copies of a chromosome formed during DNA replication) are joined together. The centromere contains highly repeated DNA sequences and proteins that form a complex structure known as the kinetochore, which serves as an attachment site for microtubules of the mitotic spindle during cell division.
During mitosis or meiosis, the kinetochore facilitates the movement of chromosomes by interacting with the microtubules, allowing for the accurate distribution of genetic material to the daughter cells. Centromeres can vary in their position and structure among different species, ranging from being located near the middle of the chromosome (metacentric) to being positioned closer to one end (acrocentric). The precise location and characteristics of centromeres are essential for proper chromosome segregation and maintenance of genomic stability.
The Tobacco mosaic satellite virus (TMVsat) is a small, single-stranded RNA virus that requires the presence of the Tobacco mosaic virus (TMV) to replicate. It is not capable of independent replication and is considered a type of satellite virus or viroid. TMVsat can cause symptoms in plants infected with TMV, including mottling and discoloration of the leaves. However, it is not as damaging to the plant as the TMV itself. It is transmitted through the same means as TMV, primarily through mechanical transmission by contaminated hands, tools, or equipment.
Skeletal muscle fibers, also known as striated muscle fibers, are the type of muscle cells that make up skeletal muscles, which are responsible for voluntary movements of the body. These muscle fibers are long, cylindrical, and multinucleated, meaning they contain multiple nuclei. They are surrounded by a connective tissue layer called the endomysium, and many fibers are bundled together into fascicles, which are then surrounded by another layer of connective tissue called the perimysium.
Skeletal muscle fibers are composed of myofibrils, which are long, thread-like structures that run the length of the fiber. Myofibrils contain repeating units called sarcomeres, which are responsible for the striated appearance of skeletal muscle fibers. Sarcomeres are composed of thick and thin filaments, which slide past each other during muscle contraction to shorten the sarcomere and generate force.
Skeletal muscle fibers can be further classified into two main types based on their contractile properties: slow-twitch (type I) and fast-twitch (type II). Slow-twitch fibers have a high endurance capacity and are used for sustained, low-intensity activities such as maintaining posture. Fast-twitch fibers, on the other hand, have a higher contractile speed and force generation capacity but fatigue more quickly and are used for powerful, explosive movements.
To the best of my knowledge, "Remote Sensing Technology" is not a term that has a specific medical definition. Remote sensing technology is a broad term that refers to the use of sensors and instruments to measure and collect data about an object or area without coming into physical contact with it. This technology is often used in fields such as geography, ecology, and agriculture to gather information about large areas of land or water. It is not typically associated with medical definitions or applications.
A human genome is the complete set of genetic information contained within the 23 pairs of chromosomes found in the nucleus of most human cells. It includes all of the genes, which are segments of DNA that contain the instructions for making proteins, as well as non-coding regions of DNA that regulate gene expression and provide structural support to the chromosomes.
The human genome contains approximately 3 billion base pairs of DNA and is estimated to contain around 20,000-25,000 protein-coding genes. The sequencing of the human genome was completed in 2003 as part of the Human Genome Project, which has had a profound impact on our understanding of human biology, disease, and evolution.
"Immobilized antibodies" refer to antibodies that have been fixed or attached to a solid support or surface. This is often done for use in various diagnostic and research applications, such as immunoassays, biosensors, and affinity chromatography. The immobilization of antibodies allows them to capture and detect specific target molecules (antigens) from complex samples, while remaining stationary and easily recoverable for reuse.
There are several methods for immobilizing antibodies, including physical adsorption, covalent attachment, and non-covalent entrapment. The choice of method depends on the specific application and the desired properties of the immobilized antibodies, such as stability, orientation, and accessibility.
It is important to note that the immobilization process may affect the binding affinity and specificity of the antibodies, and therefore careful optimization and validation are necessary to ensure the performance of the assay or application.
A genome is the complete set of genetic material (DNA, or in some viruses, RNA) present in a single cell of an organism. It includes all of the genes, both coding and noncoding, as well as other regulatory elements that together determine the unique characteristics of that organism. The human genome, for example, contains approximately 3 billion base pairs and about 20,000-25,000 protein-coding genes.
The term "genome" was first coined by Hans Winkler in 1920, derived from the word "gene" and the suffix "-ome," which refers to a complete set of something. The study of genomes is known as genomics.
Understanding the genome can provide valuable insights into the genetic basis of diseases, evolution, and other biological processes. With advancements in sequencing technologies, it has become possible to determine the entire genomic sequence of many organisms, including humans, and use this information for various applications such as personalized medicine, gene therapy, and biotechnology.
Deoxyribonucleic acid (DNA) is the genetic material present in the cells of organisms where it is responsible for the storage and transmission of hereditary information. DNA is a long molecule that consists of two strands coiled together to form a double helix. Each strand is made up of a series of four nucleotide bases - adenine (A), guanine (G), cytosine (C), and thymine (T) - that are linked together by phosphate and sugar groups. The sequence of these bases along the length of the molecule encodes genetic information, with A always pairing with T and C always pairing with G. This base-pairing allows for the replication and transcription of DNA, which are essential processes in the functioning and reproduction of all living organisms.
The Human Genome Project (HGP) is a large-scale international scientific research effort to determine the base pair sequence of the entire human genome, reveal the locations of every gene, and map all of the genetic components associated with inherited diseases. The project was completed in 2003, two years ahead of its original schedule.
The HGP has significantly advanced our understanding of human genetics, enabled the identification of genetic variations associated with common and complex diseases, and paved the way for personalized medicine. It has also provided a valuable resource for biological and medical research, as well as for forensic science and other applications.
A bacterial genome is the complete set of genetic material, including both DNA and RNA, found within a single bacterium. It contains all the hereditary information necessary for the bacterium to grow, reproduce, and survive in its environment. The bacterial genome typically includes circular chromosomes, as well as plasmids, which are smaller, circular DNA molecules that can carry additional genes. These genes encode various functional elements such as enzymes, structural proteins, and regulatory sequences that determine the bacterium's characteristics and behavior.
Bacterial genomes vary widely in size, ranging from around 130 kilobases (kb) in Mycoplasma genitalium to over 14 megabases (Mb) in Sorangium cellulosum. The complete sequencing and analysis of bacterial genomes have provided valuable insights into the biology, evolution, and pathogenicity of bacteria, enabling researchers to better understand their roles in various diseases and potential applications in biotechnology.
Satellite DNA
Indian Regional Navigation Satellite System
Tengiz Beridze
Kadal (2013 film)
Non-coding DNA
Macrosatellite
Buoyant density centrifugation
2007 in spaceflight
Hammerhead ribozyme
Tandemly arrayed genes
Marianne Frommer
Central Manchester University Hospitals NHS Foundation Trust
Euchromatin
Dhoom 3
Life Is Beautiful (2012 film)
PK (film)
Marian Carlson
Human satellite II
Satellite glial cell
Indian Telegraph Act, 1885
Extrachromosomal DNA
Centric heterochromatin
Colobopsis anderseni
Neocentromere
Nanosatellite Launch System
Operation Fishbowl
Mumbai-Ahmedabad high-speed rail corridor
Extrachromosomal circular DNA
Repeated sequence (DNA)
JASON (advisory group)
Sequences11
- Sequences with a greater ratio of A+T display a lower density while those with a greater ratio of G+C display a higher density than the bulk of genomic DNA. (wikipedia.org)
- Some repetitive sequences are ~50% G+C/A+T and thus have buoyant densities the same as bulk genomic DNA. (wikipedia.org)
- Several RU sequences were cloned and sequenced to reveal conserved regions of conventional DNA sequences over stretches greater than 550 bp, interspersed with five "divergent domains" within each copy of RU. (wikipedia.org)
- The most prevalent repeated sequences in the embedded microsatellite regions were CT:AG, CCT:AGG, CCCT:AGGG, and CGCAC:GTGCG These repeating sequences were shown to adopt altered structures including triple-stranded DNA, Z-DNA, stem-loop, and other conformations under superhelical stress. (wikipedia.org)
- Chromosomal Localization of Satellite DNA Sequences Among 22 Species o" by William Modi, T. G. Fanning et al. (nova.edu)
- The obtained satellite DNA sequences and their chromosomal distribution were compared among the analysed representatives of cervid subfamilies Cervinae and Capreolinae. (cas.cz)
- Satellite DNA is a class of repetitive sequences that are organized in long arrays of tandemly repeated units in most eukaryotes. (figshare.com)
- Long considered as selfish DNA, satellite sequences are now proposed to contribute to genome integrity. (figshare.com)
- The iGenomics algorithm can reportedly map DNA sequences of viral pathogens, from strains of bird flu to the Zika virus, while also identifying mutations which could save crucial time in both diagnosis and treatment of disease. (rt.com)
- Satellite sequences from megabat. (girinst.org)
- Satellite sequences in the apple genome. (girinst.org)
DNAs3
- In situ hybridization was carried out using cloned satellite DNAs from the domestic cat and domestic dog as probes to metaphase chromosomes from 12 species of felids and 10 species of canids. (nova.edu)
- Satellite DNAs play essential roles in chromosome segregation, and, thanks to their extensive sequence variability, to some extent, they can also be used as phylogenetic markers. (cas.cz)
- Despite their potential impact on the architecture and evolution of the genome, satellite DNAs have not been investigated in oomycetes due to the paucity of genomic data and the difficulty of assembling highly conserved satellite arrays. (figshare.com)
20211
- In 2021, BGI offered to set up COVID test centers in the United States, but U.S. security officials warned that test centers would allow China to gain access to American DNA, as the swabs have genetic material on them. (theepochtimes.com)
Genomic3
- The name "satellite DNA" refers to the phenomenon that repetitions of a short DNA sequence tend to produce a different frequency of the bases adenine, cytosine, guanine, and thymine, and thus have a different density from bulk DNA such that they form a second or "satellite" band(s) when genomic DNA is separated along a cesium chloride density gradient using buoyant density centrifugation. (wikipedia.org)
- These satellites are called "cryptic" satellites because they form a band hidden within the main band of genomic DNA. (wikipedia.org)
- Strikingly, some of these cells display selective template-DNA strand segregation during mitosis in the muscle fibre in vivo, as well as in culture independent of their niche, indicating that genomic DNA strands are nonequivalent. (nih.gov)
Orbit4
- The group of six climate research satellites crosses the equator from north to south at 1:30 p.m. local time on each orbit. (phys.org)
- A third type of swarm might combine both, with some satellites in the same orbit, following one another with some time offset, and other satellites which may be in orbits with different altitudes and/or inclinations. (scitechdaily.com)
- EDT Thursday (1811 GMT, May 6, or 2:11 a.m. local time May 7), sending the eighth trio of Yaogan-30 satellites into a 370-mile-altitude (600 kilometers) orbit. (livescience.com)
- The Long March 4C rocket lifted off from the Jiuquan Satellite Launch Center in the Gobi Desert of northwest China, placing Yaogan-34 into a 680-mile-high (1,100 km) orbit. (livescience.com)
Genome6
- Satellite DNA adopts higher-order three-dimensional structures in a naturally occurring complex satellite DNA from the land crab Gecarcinus lateralis, whose genome contains 3% of a GC-rich satellite band consisting of a ~2100 base pair (bp) "repeat unit" sequence motif called RU. (wikipedia.org)
- This unprecedented coverage of ultra-long reads enabled the resolution of most repeats in the genome, including large fractions of the centromeric satellite arrays and short arms of the acrocentrics. (labroots.com)
- Background: The full catalog of satellite DNA (satDNA) within a same genome constitutes the satellitome. (uea.ac.uk)
- Here we report a comparative analysis of genome-wide DNA polymorphism data for population pairs from eighteen Indo-Pacific bird species from the Moluccan islands of Buru and Seram and from across the island of New Guinea. (scilifelab.se)
- DNA transposons from the apple genome. (girinst.org)
- How much of the human genome is dispersed repetitive DNA? (brainscape.com)
Chromosomes1
- The absence or low intensity of satellite DNA FISH signals on biarmed chromosomes probably reflects the evolutionary reduction of heterochromatin following the formation of chromosome fusions. (cas.cz)
Sequence3
- Low-resolution sequencing-based studies have demonstrated variation in human population satellite array lengths as well as in the frequency of certain sequence and structural variations (11-13, 29). (wikipedia.org)
- The satDNAs shared by both species showed poor association with sequence signatures and motives frequently argued as functional, except for short inverted repeats allowing short dyad symmetries and non-B DNA conformations. (uea.ac.uk)
- nine patients with skin lesions had DNA sequence signatures specific for monkeypox. (cdc.gov)
Centromeric4
- Most satellite DNA is localized to the telomeric or the centromeric region of the chromosome. (wikipedia.org)
- These avian species highlighted the presence of a GC-rich Corvoidea satellitome composed of 61 satellite families and provided a set of candidate satDNA monomers for being centromeric on the basis of length, abundance, homogeneity and transcription. (scilifelab.se)
- Centromeric and pericentromeric chromosome regions are occupied by satellite DNA. (cas.cz)
- Several indices, including FISH patterns, the high guanine and cytosine (GC) content, and the presence of centromere protein B (CENP-B) binding motif, suggest that the satII DNA may represent the most important satellite DNA family that might be involved in the centromeric function in Cervidae. (cas.cz)
Centromere1
- However, due to a lack of full centromere assemblies, base-level understanding of satellite array variation and evolution has remained weak. (wikipedia.org)
SatDNA1
- Satellite DNA (satDNA) is a fast-evolving portion of eukaryotic genomes. (scilifelab.se)
Strands3
- The cosegregation of 'immortal' template DNA strands and their link with the asymmetry apparatus has important implications for stem cell biology and cancer. (nih.gov)
- Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
- During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
Chromosomal2
Evolution1
- Satellite DNA evolution in Corvoidea inferred from short and long reads. (scilifelab.se)
Replication1
- citation needed] Microsatellites are thought to have originated by polymerase slippage during DNA replication. (wikipedia.org)
Mitochondrial3
- The researchers found that a common mitochondrial DNA mutation plays a role in obesity and other health issues associated with a diet high in carbohydrates. (earth.com)
- Fruit fly larvae with a specific mitochondrial DNA (mtDNA) mutation showed a substantial increase in development when they were fed the high-carbohydrate banana diet. (earth.com)
- Independent mitochondrial DNA is altered in this genetic disorder? (brainscape.com)
Microsatellite3
- Satellite DNA, together with minisatellite and microsatellite DNA, constitute the tandem repeats. (wikipedia.org)
- Using the microsatellite DNA polymorphisms (STRPs) D14S53, D14S55, D14S48, and D14S45, we found significantly positive lod scores in 16 Portuguese kindreds, suggesting that the MJD locus is linked to chromosome 14q in this population. (nih.gov)
- A report from the Institute of Microsatellite Innovation of the Chinese Academy of Sciences (CAS) states that the Yaogan-30 (08) satellites have a new multi-satellite network operation mode, mainly used for "electromagnetic environment surveys and related technical tests. (livescience.com)
Constituent1
- Satellite DNA is the main component of functional centromeres, and form the main structural constituent of heterochromatin. (wikipedia.org)
Centromeres1
- Composed of tightly packaged, rapidly evolving satellite DNA with conserved functions, centromeres pose conundrums on several levels. (genengnews.com)
Genes1
- Loss of expression of the respec- rectal cancers [4,5] and is known to tive mismatch repair genes protein arise due to mutations in DNA mis- Tissue blocks of 32 different patients was defined as absence of nuclear match repair genes [6]. (who.int)
Arrays2
- Satellite DNA consists of very large arrays of tandemly repeating, non-coding DNA. (wikipedia.org)
- The size of satellite DNA arrays varies greatly between individuals. (wikipedia.org)
Researchers4
- "Today, we all carry professional cameras in our pockets, so it's not that hard to imagine in the next couple years, all of us carrying our own DNA sequencers on our smartphones, as well," the researchers write, adding that people may soon be able to scan themselves for pathogens using just their smartphones, which may prove crucial in a post-Covid-19 era. (rt.com)
- Leveraging data from the TROPOspheric Monitoring Instrument (TROPOMI) aboard the European Space Agency's Copernicus Sentinel-5 Precursor satellite (S-5P), researchers honed in on nitrogen dioxide emissions, a primary component of smog. (earth.com)
- As NCAR scientist and study lead author Sara Martínez-Alonso notes, the satellite observations enabled researchers to not only quantify emissions but also put them in context for the affected regions. (earth.com)
- However, when the researchers applied DNA damage stress, they observed the mutant with Rabl-structural configuration grew organs at a slower rate than the normal plant. (genengnews.com)
Nucleotides1
- For example, minisatellite DNA is a short region (1-5kb) of repeating elements with length >9 nucleotides. (wikipedia.org)
Species1
- In this paper, we isolated and sequenced satellite DNA I-IV in 11 species of Cervidae. (cas.cz)
Centre1
- En 1990, il est recruté par le Salk Institute de San Diego pour lancer un centre de recherche sur le SIDA. (epfl.ch)
Humans1
- The major satellite DNA families in humans are called: A repeated pattern can be between 1 base pair long (a mononucleotide repeat) to several thousand base pairs long, and the total size of a satellite DNA block can be several megabases without interruption. (wikipedia.org)
Human2
- Some joker put human DNA on a satellite but guess what? (killingthebuddha.com)
- The information that the brain produces is transmitted to some satellites with some agencies and organizations to study the human race "read" thoughts. (ning.com)
Viral1
- Monkeypox specific DNA signatures also were found in a viral isolate derived from lymphoid tissue of a patient's ill prairie dog. (cdc.gov)
Observations1
- However, a small satellite covering a large area cannot make high spatial resolution observations. (scitechdaily.com)
Patterns3
- Swarms of small satellites could communicate with one another to collect data on important weather patterns at different times of the day or year, and from multiple angles. (scitechdaily.com)
- The satellite data showed distinct patterns of emissions corresponding to individual copper and cobalt mines. (earth.com)
- A geostationary satellite, which remains fixed over a specific location, could offer hourly updates on emission levels, thereby painting a much clearer picture of pollution patterns and sources. (earth.com)
Data3
- A new satellite that last week began gathering data from the Earth's atmosphere could be a key tool in unraveling just how much effect the reflectivity of clouds and tiny particles called aerosols are having on the planet's changing climate. (phys.org)
- CALIPSO is fourth in the formation, traveling just a few seconds behind a satellite called CloudSat, which was launched on the same rocket and will provide radar data on thick clouds, complementing CALIPSO's lidar data on thin clouds and aerosols. (phys.org)
- Also aboard the flight was Tianqi-12, a small satellite for Internet of Things (IoT) data connectivity for the Beijing-based commercial company Guodian Gaoke. (livescience.com)
Scientists2
- Scientists around the world hope to begin getting answers from the Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation satellite, dubbed CALIPSO, which was launched from Vandenberg Air Force Base in California on April 28. (phys.org)
- Using the new and rapidly improving ability to piece together fragments of ancient DNA, scientists are finding that traits inherited from Neanderthals are still with us now, affecting our fertility, our immune systems, even how our bodies handled the COVID-19 virus. (ctvnews.ca)
Assembly1
- The new funds from Lockheed will go toward acquiring additional assembly space and increasing satellite module production, Terran said in a statement. (yahoo.com)
Cells6
- Satellite cells assure postnatal skeletal muscle growth and repair. (nih.gov)
- Using pulse-chase labelling with BrdU to mark the putative stem cell niche, we identify a subpopulation of label-retaining satellite cells during growth and after injury. (nih.gov)
- Finally, we show that template DNA cosegregates with Numb in label-retaining cells that express the self-renewal marker Pax7. (nih.gov)
- Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
- Crosstalk between H3K9me3 and DNA methylation was examined in cells transfected with siRNA of histone trimethyl-transferase, Suv39H1, or Dnmt1 , exposed to high glucose. (nature.com)
- Mismatch repair proteins correct King Khalid University Hospital, King mal nuclear staining in lymphocytes the insertion and deletion mutations Saud University, Riyadh, Saudi Arabia and normal epithelial or stromal cells that occur when DNA is copied be- between 2007 and 2009 were retrieved was required serving as internal con- fore cell division. (who.int)
Constellation2
- CALIPSO, the fourth satellite in the so-called 'A-Train' constellation, follows just a few seconds behind Cloudsat, the satellite it was launched with on April 28. (phys.org)
- The company was originally planning to launch and operate its own SAR satellite constellation, called PredaSAR, but it decided to pivot from those plans and offer the technology as a product instead. (yahoo.com)
Constitutes1
- If virus (Latin: slimy) challenges the definition of what constitutes life, the DNA mimivirus tests how we define virus. (cdc.gov)
Collect1
- Two satellites on similar orbits collect valuable perspectives on the same part of the atmosphere. (scitechdaily.com)
Families2
- Among them, two abundant families, designated as PpSat1 and PpSat2, displayed typical features of satellite DNA and were collectively designated as PpSat. (figshare.com)
- This observation, as well as the characterization of transcripts of PpSat families, suggested that these satellite DNA families likely play a conserved role within this important group of pathogens. (figshare.com)
Phenomenon2
- How do you tell a swarm of satellites what region and time of day is the best to observe that phenomenon? (scitechdaily.com)
- Ideally, I like to have a satellite with a wide field of view observing larger phenomenon," he said. (scitechdaily.com)
Evolutionary1
- There is a lot that evolutionary biologists can learn about the purpose of junk DNA from my wife. (reasons.org)
Region3
- The difference in how many of the repeats is present in the region (length of the region) is the basis for DNA profiling. (wikipedia.org)
- This research reveals that satellites can detect and quantify the emissions from mining operations in this region. (earth.com)
- Currently, such satellites do not cover Africa or any other region in the global South. (earth.com)
Bird1
- The code names of the now-disabled satellites are Snow White, Corona and Big Bird, followed by a one or two-digit number. (divinecosmos.com)
Tests2
- Home ancestry tests are another way that the Chinese regime is obtaining DNA from Americans. (theepochtimes.com)
- The satellites will be used for electromagnetic environment surveys and other related technology tests, according to state media Xinhua . (livescience.com)
Long4
- China conducted two launches of classified Yaogan satellites in the last week while much of the world waited to see where and when the Long March 5B would fall. (livescience.com)
- A Long March 2C rocket lifted off from the Xichang Satellite Launch Center in southwest China at 2:11 p.m. (livescience.com)
- Liftoff of the Long March 2C from Xichang carrying three Yaogan-30 (08) satellites and Tianqi-12. (livescience.com)
- The Yaogan-30 (08) mission was preceded by a Long March 4C launch of the Yaogan-34 satellite on April 30 at 3:27 a.m. (livescience.com)
Military2
- MOSCOW (AP) - Russian President Vladimir Putin told a senior Chinese military official Wednesday that Moscow and Beijing should expand their cooperation on military satellites and other prospective defense technologies- a statement that signaled increasingly close defense links between the allies. (everythinglubbock.com)
- Yaogan series satellites are understood by western analysts to be Chinese military reconnaissance satellites. (livescience.com)
Rocket2
Communications1
- These covert satellites were apparently the communications backbone of the Cabal / New World Order / Illuminati / Deep State. (divinecosmos.com)
Growth1
- Using satellite technology to monitor such regions can yield insights into the environmental consequences of mining and also serve as a tool for estimating the growth of such activities in remote regions, which might otherwise be underreported or unmonitored. (earth.com)
Launch1
- The mission poster for the launch of NROL-25, a new spy satellite for the National Reconnaissance Office, on April 3, 2012. (space.com)
Markers1
- Such allodynia was associated with elevation of markers for activated microglia/macrophages (CD11b) and astrocytes/satellite glia (GFAP) in the lumbar dorsal spinal cord and dorsal root ganglia (DRG). (cdc.gov)
Protein1
- A small molecular weight G-protein essential for Nox2 activation, Rac1, is also transcriptionally activated via active DNA methylation-hydroxymethylation. (nature.com)
Soldiers1
- After manipulating generations of pig DNA, intentionally producing pigs that are smaller or larger, more susceptible to certain diseases, or less susceptible to others, the CCP is zeroing in on the ability to produce "super soldiers. (theepochtimes.com)
Contract1
- Boca Raton, Florida-based Terran Orbital is a contract manufacturer, designing and building satellites for the U.S. government and commercial customers. (yahoo.com)
Institute1
- Après des études de médecine à l'Université de Genève et une formation clinique en pathologie, médecine interne et maladies infectieuses à Genève et au Massachusetts General Hospital de Boston, Didier Trono s'engage dans une carrière scientifique au Whitehead Institute du MIT. (epfl.ch)
Space3
- After establishing communication with the satellite in September of 2013, ground controllers commanded the craft to point the telescope towards deep space to start radiating away its accumulated heat. (hackaday.com)
- There's a lot of interest to do DNA sequencing in space. (rt.com)
- Iran claimed on Wednesday that it successfully launched an imaging satellite into space, a move that could further ratchet up tensions with Western nations that fear its space technology could be used to develop nuclear weapons. (ctvnews.ca)
Role2
- Our aim is to investigate the role of histone methylation (H3K9me3) of Rac1 promoter in dynamic DNA methylation- transcriptional activation. (nature.com)
- Demonstrating a casual role for such neuroinflammatory signaling, allodynia was reversed by treatment with either minocycline, the TLR4 inhibitor (+)-naltrexone, or IL-10 plasmid DNA. (cdc.gov)
Regions1
- however, certain C-band-positive regions without grains probably do not contain the particular satellites studied here. (nova.edu)
Chinese3
- Chinese medical testing companies regularly use DNA collected from test subjects for other research. (theepochtimes.com)
- In addition, Chinese police are trying to amass samples of DNA from the country's 700 million males, to keep track of future criminals. (theepochtimes.com)
- The satellites' lifespan and reliability have also been improved over those previously launched, the report says (Chinese) . (livescience.com)
Specific1
- CALIPSO will use lidar -- a measuring tool like radar except that it employs laser light rather than radio waves -- to measure the specific altitudes of clouds and aerosols to within about 100 feet, a groundbreaking advance on current satellite capabilities. (phys.org)
Years1
- Satellites for many years have produced pictures showing the Earth swathed in clouds. (phys.org)