Plants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Plant Roots: The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)Mouth, Edentulous: Total lack of teeth through disease or extraction.Plant Extracts: Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.Plant Shoots: New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.Plants, Medicinal: Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.DNA, Plant: Deoxyribonucleic acid that makes up the genetic material of plants.Plant Development: Processes orchestrated or driven by a plethora of genes, plant hormones, and inherent biological timing mechanisms facilitated by secondary molecules, which result in the systematic transformation of plants and plant parts, from one stage of maturity to another.Plants, Toxic: Plants or plant parts which are harmful to man or other animals.Plant Cells: Basic functional unit of plants.Plant Stems: Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)Genome, Plant: The genetic complement of a plant (PLANTS) as represented in its DNA.Arabidopsis: A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.Plants, Edible: An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.Plant Structures: The parts of plants, including SEEDS.Plant Growth Regulators: Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins.Arabidopsis Proteins: Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.Plant Immunity: The inherent or induced capacity of plants to withstand or ward off biological attack by pathogens.Tobacco: A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.Plant Epidermis: A thin layer of cells forming the outer integument of seed plants and ferns. (Random House Unabridged Dictionary, 2d ed)Plant Stomata: Closable openings in the epidermis of plants on the underside of leaves. They allow the exchange of gases between the internal tissues of the plant and the outside atmosphere.Angiosperms: Members of the group of vascular plants which bear flowers. They are differentiated from GYMNOSPERMS by their production of seeds within a closed chamber (OVARY, PLANT). The Angiosperms division is composed of two classes, the monocotyledons (Liliopsida) and dicotyledons (Magnoliopsida). Angiosperms represent approximately 80% of all known living plants.Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Plant Poisoning: Poisoning by the ingestion of plants or its leaves, berries, roots or stalks. The manifestations in both humans and animals vary in severity from mild to life threatening. In animals, especially domestic animals, it is usually the result of ingesting moldy or fermented forage.Plant Transpiration: The loss of water vapor by plants to the atmosphere. It occurs mainly from the leaves through pores (stomata) whose primary function is gas exchange. The water is replaced by a continuous column of water moving upwards from the roots within the xylem vessels. (Concise Dictionary of Biology, 1990)Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Lycopersicon esculentum: A plant species of the family SOLANACEAE, native of South America, widely cultivated for their edible, fleshy, usually red fruit.Flowers: The reproductive organs of plants.Plant Tumors: A localized proliferation of plant tissue forming a swelling or outgrowth, commonly with a characteristic shape and unlike any organ of the normal plant. Plant tumors or galls usually form in response to the action of a pathogen or a pest. (Holliday, P., A Dictionary of Plant Pathology, 1989, p330)Chromosomes, Plant: Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.Plant Components, Aerial: The above-ground plant without the roots.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Plant Preparations: Material prepared from plants.Zea mays: A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.Seedling: Very young plant after GERMINATION of SEEDS.Herbivory: The act of feeding on plants by animals.Power Plants: Units that convert some other form of energy into electrical energy.Biomass: Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.Fabaceae: The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.Plant Nectar: Sugar-rich liquid produced in plant glands called nectaries. It is either produced in flowers or other plant structures, providing a source of attraction for pollinating insects and animals, as well as being a nutrient source to animal mutualists which provide protection of plants against herbivores.Oxylipins: Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Plant Bark: The outer layer of the woody parts of plants.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Photosynthesis: The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)Plant Physiological Processes: Physiological functions characteristic of plants.Apgar Score: A method, developed by Dr. Virginia Apgar, to evaluate a newborn's adjustment to extrauterine life. Five items - heart rate, respiratory effort, muscle tone, reflex irritability, and color - are evaluated 60 seconds after birth and again five minutes later on a scale from 0-2, 0 being the lowest, 2 being normal. The five numbers are added for the Apgar score. A score of 0-3 represents severe distress, 4-7 indicates moderate distress, and a score of 7-10 predicts an absence of difficulty in adjusting to extrauterine life.Indoleacetic Acids: Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)Solanum tuberosum: A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.Soil: The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.Medicine, Traditional: Systems of medicine based on cultural beliefs and practices handed down from generation to generation. The concept includes mystical and magical rituals (SPIRITUAL THERAPIES); PHYTOTHERAPY; and other treatments which may not be explained by modern medicine.Plant Exudates: Substances released by PLANTS such as PLANT GUMS and PLANT RESINS.Ecosystem: A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Salicylic Acid: A compound obtained from the bark of the white willow and wintergreen leaves. It has bacteriostatic, fungicidal, and keratolytic actions.Root Nodules, Plant: Knobbed structures formed from and attached to plant roots, especially of LEGUMES, which result from symbiotic infection by nitrogen fixing bacteria such as RHIZOBIUM or FRANKIA. Root nodules are structures related to MYCORRHIZAE formed by symbiotic associations with fungi.Plant Lectins: Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.Necrosis: The pathological process occurring in cells that are dying from irreparable injuries. It is caused by the progressive, uncontrolled action of degradative ENZYMES, leading to MITOCHONDRIAL SWELLING, nuclear flocculation, and cell lysis. It is distinct it from APOPTOSIS, which is a normal, regulated cellular process.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Mycorrhizae: Symbiotic combination (dual organism) of the MYCELIUM of FUNGI with the roots of plants (PLANT ROOTS). The roots of almost all higher plants exhibit this mutually beneficial relationship, whereby the fungus supplies water and mineral salts to the plant, and the plant supplies CARBOHYDRATES to the fungus. There are two major types of mycorrhizae: ectomycorrhizae and endomycorrhizae.Phytotherapy: Use of plants or herbs to treat diseases or to alleviate pain.Poaceae: A large family of narrow-leaved herbaceous grasses of the order Cyperales, subclass Commelinidae, class Liliopsida (monocotyledons). Food grains (EDIBLE GRAIN) come from members of this family. RHINITIS, ALLERGIC, SEASONAL can be induced by POLLEN of many of the grasses.Chloroplasts: Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.Plant Infertility: The failure of PLANTS to complete fertilization and obtain seed (SEEDS) as a result of defective POLLEN or ovules, or other aberrations. (Dict. of Plant Genet. and Mol. Biol., 1998)Pollen: The fertilizing element of plants that contains the male GAMETOPHYTES.Brassica: A plant genus of the family Cruciferae. It contains many species and cultivars used as food including cabbage, cauliflower, broccoli, Brussel sprouts, kale, collard greens, MUSTARD PLANT; (B. alba, B. junica, and B. nigra), turnips (BRASSICA NAPUS) and rapeseed (BRASSICA RAPA).Germination: The initial stages of the growth of SEEDS into a SEEDLINGS. The embryonic shoot (plumule) and embryonic PLANT ROOTS (radicle) emerge and grow upwards and downwards respectively. Food reserves for germination come from endosperm tissue within the seed and/or from the seed leaves (COTYLEDON). (Concise Dictionary of Biology, 1990)Asteraceae: A large plant family of the order Asterales, subclass Asteridae, class Magnoliopsida. The family is also known as Compositae. Flower petals are joined near the base and stamens alternate with the corolla lobes. The common name of "daisy" refers to several genera of this family including Aster; CHRYSANTHEMUM; RUDBECKIA; TANACETUM.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Plant Oils: Oils derived from plants or plant products.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Droughts: Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors.Botany: The study of the origin, structure, development, growth, function, genetics, and reproduction of plants.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Peas: A variable annual leguminous vine (Pisum sativum) that is cultivated for its rounded smooth or wrinkled edible protein-rich seeds, the seed of the pea, and the immature pods with their included seeds. (From Webster's New Collegiate Dictionary, 1973)Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Triticum: A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.Abscisic Acid: Abscission-accelerating plant growth substance isolated from young cotton fruit, leaves of sycamore, birch, and other plants, and from potatoes, lemons, avocados, and other fruits.Phytosterols: A class of organic compounds known as STEROLS or STEROIDS derived from plants.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Hordeum: A plant genus of the family POACEAE. The EDIBLE GRAIN, barley, is widely used as food.Pseudomonas syringae: A species of gram-negative, fluorescent, phytopathogenic bacteria in the genus PSEUDOMONAS. It is differentiated into approximately 50 pathovars with different plant pathogenicities and host specificities.Bryopsida: A class of plants within the Bryophyta comprising the mosses, which are found in both damp (including freshwater) and drier situations. Mosses possess erect or prostrate leafless stems, which give rise to leafless stalks bearing capsules. Spores formed in the capsules are released and grow to produce new plants. (Concise Dictionary of Biology, 1990). Many small plants bearing the name moss are in fact not mosses. The "moss" found on the north side of trees is actually a green alga (CHLOROPHYTA). Irish moss is really a red alga (RHODOPHYTA). Beard lichen (beard moss), Iceland moss, oak moss, and reindeer moss are actually LICHENS. Spanish moss is a common name for both LICHENS and an air plant (TILLANDSIA usneoides) of the pineapple family. Club moss is an evergreen herb of the family LYCOPODIACEAE.Light: That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.Concurrent Review: Review of the medical necessity of hospital or other health facility admissions, upon or within a short time following an admission, and periodic review of services provided during the course of treatment.Protoplasts: The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.Hemoglobin E: An abnormal hemoglobin that results from the substitution of lysine for glutamic acid at position 26 of the beta chain. It is most frequently observed in southeast Asian populations.Chlorophyll: Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms.Meristem: A group of plant cells that are capable of dividing infinitely and whose main function is the production of new growth at the growing tip of a root or stem. (From Concise Dictionary of Biology, 1990)Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Agrobacterium tumefaciens: A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.Plastids: Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.Aphids: A family (Aphididae) of small insects, in the suborder Sternorrhyncha, that suck the juices of plants. Important genera include Schizaphis and Myzus. The latter is known to carry more than 100 virus diseases between plants.Rhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.Trees: Woody, usually tall, perennial higher plants (Angiosperms, Gymnosperms, and some Pterophyta) having usually a main stem and numerous branches.Transformation, Genetic: Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.Volatile Organic Compounds: Organic compounds that have a relatively high VAPOR PRESSURE at room temperature.Cucumis sativus: A creeping annual plant species of the CUCURBITACEAE family. It has a rough succulent, trailing stem and hairy leaves with three to five pointed lobes.Cytokinins: Plant hormones that promote the separation of daughter cells after mitotic division of a parent cell. Frequently they are purine derivatives.Hydroponics: A technique for growing plants in culture solutions rather than in soil. The roots are immersed in an aerated solution containing the correct proportions of essential mineral salts. (From Concise Dictionary of Biology, 1990)Mustard Plant: Any of several BRASSICA species that are commonly called mustard. Brassica alba is white mustard, B. juncea is brown or Chinese mustard, and B. nigra is black, brown, or red mustard. The plant is grown both for mustard seed from which oil is extracted or used as SPICES, and for its greens used as VEGETABLES or ANIMAL FEED. There is no relationship to MUSTARD COMPOUNDS.Stress, Physiological: The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions.Hemiptera: A large order of insects characterized by having the mouth parts adapted to piercing or sucking. It is comprised of four suborders: HETEROPTERA, Auchenorrhyncha, Sternorrhyncha, and Coleorrhyncha.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Endophytes: An endosymbiont that is either a bacterium or fungus living part of its life in a plant. Endophytes can benefit host plants by preventing pathogenic organisms from colonizing them.Biodiversity: The variety of all native living organisms and their various forms and interrelationships.Solanaceae: A plant family of the order Solanales, subclass Asteridae. Among the most important are POTATOES; TOMATOES; CAPSICUM (green and red peppers); TOBACCO; and BELLADONNA.Adaptation, Physiological: The non-genetic biological changes of an organism in response to challenges in its ENVIRONMENT.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Bryophyta: A division of the plant kingdom. Bryophyta contains the subdivision, Musci, which contains the classes: Andreaeopsida, BRYOPSIDA, and SPHAGNOPSIDA.Ferns: Seedless nonflowering plants of the class Filicinae. They reproduce by spores that appear as dots on the underside of feathery fronds. In earlier classifications the Pteridophyta included the club mosses, horsetails, ferns, and various fossil groups. In more recent classifications, pteridophytes and spermatophytes (seed-bearing plants) are classified in the Subkingdom Tracheobionta (also known as Tracheophyta).Plant Dispersal: The physical distribution of plants in various forms and stages of development through time and space.Ascomycota: A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Xylem: Plant tissue that carries water up the root and stem. Xylem cell walls derive most of their strength from LIGNIN. The vessels are similar to PHLOEM sieve tubes but lack companion cells and do not have perforated sides and pores.Gibberellins: A class of plant growth hormone isolated from cultures of Gibberella fujikuroi, a fungus causing Bakanae disease in rice. There are many different members of the family as well as mixtures of multiple members; all are diterpenoid acids based on the gibberellane skeleton.Medicago sativa: A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.Gymnosperms: Gymnosperms are a group of vascular plants whose seeds are not enclosed by a ripened ovary (fruit), in contrast to ANGIOSPERMS whose seeds are surrounded by an ovary wall. The seeds of many gymnosperms (literally, "naked seed") are borne in cones and are not visible. Taxonomists now recognize four distinct divisions of extant gymnospermous plants (CONIFEROPHYTA; CYCADOPHYTA; GINKGOPHYTA; and GNETOPHYTA).Nuclear Power Plants: Facilities that convert NUCLEAR ENERGY into electrical energy.Fruit: The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Plantago: A plant genus of the family Plantaginaceae. The small plants usually have a dense tuft of basal leaves and long, leafless stalks bearing a terminal spike of small flowers. The seeds, known as PSYLLIUM, swell in water and are used as laxatives. The leaves have been used medicinally.Medicine, African Traditional: A system of traditional medicine which is based on the beliefs and practices of the African peoples. It includes treatment by medicinal plants and other materia medica as well as by the ministrations of diviners, medicine men, witch doctors, and sorcerers.Asparagus Plant: A plant genus in the family LILIACEAE (sometimes placed in Asparagaceae) that contains ECDYSTEROIDS and is an ingredient of Siotone. The shoots are used as a vegetable and the roots are used in FOLK MEDICINE.Cotyledon: A part of the embryo in a seed plant. The number of cotyledons is an important feature in classifying plants. In seeds without an endosperm, they store food which is used in germination. In some plants, they emerge above the soil surface and become the first photosynthetic leaves. (From Concise Dictionary of Biology, 1990)Lamiaceae: The mint plant family. They are characteristically aromatic, and many of them are cultivated for their oils. Most have square stems, opposite leaves, and two-lipped, open-mouthed, tubular corollas (united petals), with five-lobed, bell-like calyxes (united sepals).Haemophilus somnus: A species of gram-negative bacteria (currently incertae sedis) causing multisystem disease in CATTLE.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Muscle, Smooth, Vascular: The nonstriated involuntary muscle tissue of blood vessels.Cucurbita: A plant genus of the family CUCURBITACEAE, order Violales, subclass Dilleniidae, which includes pumpkin, gourd and squash.Porcine Postweaning Multisystemic Wasting Syndrome: A worldwide emerging disease of weaned piglets first recognized in swine herds in western Canada in 1997. This syndrome is characterized by progressive weight loss, rapid (tachypnea) and difficult (dyspnea) breathing, and yellowing of skin. PMWS is caused by PORCINE CIRCOVIRUS infection, specifically type 2 or PCV-2.Hypocotyl: The region of the stem beneath the stalks of the seed leaves (cotyledons) and directly above the young root of the embryo plant. It grows rapidly in seedlings showing epigeal germination and lifts the cotyledons above the soil surface. In this region (the transition zone) the arrangement of vascular bundles in the root changes to that of the stem. (From Concise Dictionary of Biology, 1990)Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Agrobacterium: A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.Phaseolus: A plant genus in the family FABACEAE which is the source of edible beans and the lectin PHYTOHEMAGGLUTININS.Caulimovirus: A genus of PLANT VIRUSES, in the family CAULIMOVIRIDAE, that are transmitted by APHIDS in a semipersistent manner. Aphid-borne transmission of some caulimoviruses requires certain virus-coded proteins termed transmission factors.Brassica napus: A plant species of the family BRASSICACEAE best known for the edible roots.Medicago truncatula: A plant species of the family FABACEAE used to study GENETICS because it is DIPLOID, self fertile, has a small genome, and short generation time.Rutaceae: A plant family in the order Sapindales that grows in warmer regions and has conspicuous flowers.Host-Pathogen Interactions: The interactions between a host and a pathogen, usually resulting in disease.Carbon: A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.Botrytis: A mitosporic Leotiales fungal genus of plant pathogens. It has teleomorphs in the genus Botryotina.Embryophyta: Higher plants that live primarily in terrestrial habitats, although some are secondarily aquatic. Most obtain their energy from PHOTOSYNTHESIS. They comprise the vascular and non-vascular plants.Nuclear Power Plants: Facilities that convert NUCLEAR ENERGY into electrical energy.Lettuce: Any of the various plants of the genus Lactuca, especially L. sativa, cultivated for its edible leaves. (From American Heritage Dictionary, 2d ed)Oomycetes: Eukaryotes in the group STRAMENOPILES, formerly considered FUNGI, whose exact taxonomic level is unsettled. Many consider Oomycetes (Oomycota) a phylum in the kingdom Stramenopila, or alternatively, as Pseudofungi in the phylum Heterokonta of the kingdom Chromista. They are morphologically similar to fungi but have no close phylogenetic relationship to them. Oomycetes are found in both fresh and salt water as well as in terrestrial environments. (Alexopoulos et al., Introductory Mycology, 4th ed, pp683-4). They produce flagellated, actively motile spores (zoospores) that are pathogenic to many crop plants and FISHES.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Sitosterols: A family of sterols commonly found in plants and plant oils. Alpha-, beta-, and gamma-isomers have been characterized.Acclimatization: Adaptation to a new environment or to a change in the old.Daucus carota: A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Heavy Ions: Positively-charged atomic nuclei that have been stripped of their electrons. These particles have one or more units of electric charge and a mass exceeding that of the Helium-4 nucleus (alpha particle).Agriculture: The science, art or practice of cultivating soil, producing crops, and raising livestock.Visual Analog Scale: A subjective psychometric response scale used to measure distinct behavioral or physiological phenomena based on linear numerical gradient or yes/no alternatives.Reproduction: The total process by which organisms produce offspring. (Stedman, 25th ed)Cucurbitaceae: The gourd plant family of the order Violales, subclass Dilleniidae, class Magnoliopsida. It is sometimes placed in its own order, Cucurbitales. 'Melon' generally refers to CUCUMIS; CITRULLUS; or MOMORDICA.Genetic Variation: Genotypic differences observed among individuals in a population.Photoreceptors, Plant: Plant proteins that mediate LIGHT SIGNAL TRANSDUCTION. They are involved in PHOTOTROPISM and other light adaption responses during plant growth and development . They include the phototropins, phytochromes (PHYTOCHROME), and members of the ubiquitous cryptochrome family.Thymus Plant: A plant genus of the family LAMIACEAE best known for the thyme spice added to foods.Fusarium: A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA.Anthocyanins: A group of FLAVONOIDS derived from FLAVONOLS, which lack the ketone oxygen at the 4-position. They are glycosylated versions of cyanidin, pelargonidin or delphinidin. The conjugated bonds result in blue, red, and purple colors in flowers of plants.Sorghum: A plant genus of the family POACEAE. The grain is used for FOOD and for ANIMAL FEED. This should not be confused with KAFFIR LIME or with KEFIR milk product.Herbicides: Pesticides used to destroy unwanted vegetation, especially various types of weeds, grasses (POACEAE), and woody plants. Some plants develop HERBICIDE RESISTANCE.Carbohydrate Metabolism: Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.Bacterial Proteins: Proteins found in any species of bacterium.Conserved Sequence: A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.Araceae: A plant family of the order Arales, subclass Arecidae, class Liliopsida (monocot). Many members contain OXALIC ACID and calcium oxalate (OXALATES).Nitrogen Fixation: The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.Liver Cirrhosis, Alcoholic: FIBROSIS of the hepatic parenchyma due to chronic excess ALCOHOL DRINKING.Ribulose-Bisphosphate Carboxylase: A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.Glucosinolates: Substituted thioglucosides. They are found in rapeseed (Brassica campestris) products and related cruciferae. They are metabolized to a variety of toxic products which are most likely the cause of hepatocytic necrosis in animals and humans.Lignin: The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)Beetles: INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.Gametogenesis, Plant: The process of germ cell development in plants, from the primordial PLANT GERM CELLS to the mature haploid PLANT GAMETES.Mutagenesis, Insertional: Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.Resins, Plant: Flammable, amorphous, vegetable products of secretion or disintegration, usually formed in special cavities of plants. They are generally insoluble in water and soluble in alcohol, carbon tetrachloride, ether, or volatile oils. They are fusible and have a conchoidal fracture. They are the oxidation or polymerization products of the terpenes, and are mixtures of aromatic acids and esters. Most are soft and sticky, but harden after exposure to cold. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)Basidiomycota: A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.Salinity: Degree of saltiness, which is largely the OSMOLAR CONCENTRATION of SODIUM CHLORIDE plus any other SALTS present. It is an ecological factor of considerable importance, influencing the types of organisms that live in an ENVIRONMENT.Darkness: The absence of light.Gene Silencing: Interruption or suppression of the expression of a gene at transcriptional or translational levels.Sodium Chloride: A ubiquitous sodium salt that is commonly used to season food.Pectins: High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.Cyclic AMP Response Element Modulator: Cyclic AMP response element modulator is a basic leucine zipper transcription factor that is regulated by CYCLIC AMP. It plays an important role in SPERMATID development in the mammalian TESTIS.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Vicia faba: A plant species of the genus VICIA, family FABACEAE. The edible beans are well known but they cause FAVISM in some individuals with GLUCOSEPHOSPHATE DEHYDROGENASE DEFICIENCY. This plant contains vicine, convicine, Vicia lectins, unknown seed protein, AAP2 transport protein, and Vicia faba DNA-binding protein 1.Mesophyll Cells: Large and highly vacuolated cells possessing many chloroplasts occuring in the interior cross-section of leaves, juxtaposed between the epidermal layers.Forearm Injuries: Injuries to the part of the upper limb of the body between the wrist and elbow.Phytochrome: A blue-green biliprotein widely distributed in the plant kingdom.Selaginellaceae: A plant family of the order Selaginellales, class Lycopodiopsida, division Lycopodiophyta, subkingdom Tracheobionta. Members contain bilobetin. The rarely used common name of resurrection plant is mainly used with CRATEROSTIGMA.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Liliaceae: A monocot family within the order Liliales. This family is divided by some botanists into other families such as Convallariaceae, Hyacinthaceae and Amaryllidaceae. Amaryllidaceae, which have inferior ovaries, includes CRINUM; GALANTHUS; LYCORIS; and NARCISSUS and are known for AMARYLLIDACEAE ALKALOIDS.Fertilizers: Substances or mixtures that are added to the soil to supply nutrients or to make available nutrients already present in the soil, in order to increase plant growth and productivity.Salt-Tolerance: The ability of organisms to sense and adapt to high concentrations of salt in their growth environment.Herpesvirus 3, Gallid: A species in the genus MAREK'S DISEASE-LIKE VIRUSES, in the family HERPESVIRIDAE, infecting chickens.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Mushroom Poisoning: Poisoning from ingestion of mushrooms, primarily from, but not restricted to, toxic varieties.Citrus: A plant genus of the family RUTACEAE. They bear the familiar citrus fruits including oranges, grapefruit, lemons, and limes. There are many hybrids which makes the nomenclature confusing.Inflorescence: A cluster of FLOWERS (as opposed to a solitary flower) arranged on a main stem of a plant.
DNA isolation from plant cellDNA isolation from plant cell. Peter Gegenheimer peterg at rnaworld.bio.ukans.edu Sat Mar 19 00:56:55 EST 1994 *Previous ... Could anybody please help me with DNA isolation from plant culture cells? ,I have tried the CTAB method as usually done for ... plant tissues eg. ,leaves, although I did not freeze the cells in liquid nitrogen prior to ,grinding. I used about 7 g fresh ... when you use any similar procedure with plant LEAVES, the grinding in LN2 must NOT be omitted. I realize that if your cultured ...
Isolation of Genomic DNA from PlantSince plants contain a wide variety of metabolites, these kits offers two different lysis procedures for optimal processing of ... Isolate genomic DNA from plant tissue on a small, medium, or large scale. ... Isolation of Genomic DNA from Plant-NucleoSpin Plant II & NucleoSpin Plant Midi/Maxi. NucleoSpin Plant II is a next-generation ... The NucleoSpin Plant Midi and Plant Maxi Kits are designed for isolating genomic DNA from medium to large samples of plant ...
Magnetic Isolation of Genomic DNA from PlantRapid parallel purification of genomic DNA from plant tissues in a 96-well format. Magnetic bead technology provides a closed ... Isolation of Genomic DNA from Plant using Magnetic Beads-NucleoMag 96 Plant. NucleoMag 96 Plant is designed for the rapid ... Manual or automated isolation of DNA from plant tissue *Isolated DNA can be used for PCR, Southern blotting, any kind of ... parallel purification of genomic DNA from plant tissues in a 96-well format. After lysis of the sample, genomic DNA is ...
Plant defenses prompt bacterial countermeasure in the form of island DNA excisionPlant defenses prompt bacterial countermeasure in the form of island DNA excision. 20.12.2005 ... process of infecting a plant can shift molecular gears by excising specific genes from its genome to overcome the host plant s ... Plants and algae use the enzyme Rubisco to fix carbon dioxide, removing it from the atmosphere and converting it into biomass. ... But this genomic island also encodes enzymes that, when switched on, snip the DNA on either side of the island, resulting in ...
separate plant protoplast from bacteria prior DNA extraction - General Lab Techniquesseparate plant protoplast from bacteria prior DNA extraction - (Oct/29/2012 ). Hi all. My work is focused on microbes-plant ... does someone know a protocol to separate the plant protoplast from the bacteria to use it before performing the DNA extraction? ... I have extracted DNA from Leaves and fruits and around 95% of the sequences i obtained were from the choroplast and ... I don't know much about it so can any one tell me about DNA as well as RNA Extraction in detail.. _________. total rna ...
The role of repetitive DNA in structure and evolution of sex chromosomes in plants.Eukaryotic genomes contain a large proportion of repetitive DNA sequences, mostly transposable elements (TEs) and tandem ... Chromosomes, Plant / genetics*. Evolution, Molecular*. Plants / genetics*. Repetitive Sequences, Nucleic Acid*. Sex Chromosomes ... review the occurrence and role of repetitive DNA in Y chromosome evolution in various species with a focus on dioecious plants ... Laboratory of Plant Developmental Genetics, Institute of Biophysics ASCR, Brno, Czech Republic. firstname.lastname@example.org. ...
Contacts - Plant DNA C-values Database - Royal Botanic Gardens, KewPlant DNA C-values Database. Contacts. Plant DNA C-values Database Compilers. The Plant DNA C-values database was compiled by ... Angiosperm DNA C-values database:. MD Bennett and IJ Leitch (email@example.com). ... Pteridophyte DNA C-values database:. MD Bennett and IJ Leitch (firstname.lastname@example.org). ... Gymnosperm DNA C-values database:. B Murray (email@example.com). ...
Cell Research - Dicer-independent RNA-directed DNA methylation in ArabidopsisRNA-directed DNA methylation (RdDM) is an important de novo DNA methylation pathway in plants. Small interfering RNAs (siRNAs) ... Genomic DNA was extracted from 2-week-old seedlings using the Qiagen Plant DNeasy Mini Kit. The extracted DNA was then treated ... RNA-directed DNA methylation: the evolution of a complex epigenetic pathway in flowering plants. Annu Rev Plant Biol 2015; 66: ... Characterization of DNA methylation and siRNAs at the 5′ UTR of HEI10. (A) The methylation levels in plants of the indicated ...
Recent Articles | Dna Sequencing And Ecology | The Scientist Magazine®| Page 22Plant DNA Largely Unchanged. By Jef Akst , April 15, 2013 Today's tulip trees carry similar mitochondrial DNA as those that ... Genetics experts argue that patients should be told about dangerous variants in their DNA that show up incidentally during ...
Recent Articles | Plant Biology And Culture | The Scientist Magazine®| Page 32Following natural disasters or violent political turmoil, DNA science can help identify victims. But what if a country can't ... Two papers on plant immunity have been retracted, and questions remain about others with similar results. ... Scientists discover fossilized remnants of a flowering plant about 100 million years older than the oldest previously found. ... The crop-destroying gray mold fungus uses RNA weapons to disable plant defenses and invade. ...
Plant perception (physiology): Plant perception is the ability of plants to sense and respond to the environment to adjust their morphology, physiology and phenotype accordingly. Other disciplines such as plant physiology, ecology and molecular biology are used to assess this ability.Canna Leaf Roller: Cannas are largely free of pests, but in the USA plants sometimes fall victim the Canna Leaf Roller, which can actually be two different insects. Larva of the Brazilian skipper butterfly (Calpodes ethlius), also known as the Larger Canna Leaf Roller, cut the leaves and roll them over to live inside while pupating and eating the leaf.Endodermis: The endodermis is the central, innermost layer of cortex in some land plants. It is made of compact living cells surrounded by an outer ring of endodermal cells that are impregnated with hydrophobic substances (Casparian Strip) to restrict apoplastic flow of water to the inside.PhytomedicineMedicinal plants of the American West: Many plants that grow in the American West have use in traditional and herbal medicine.Revegetation: Revegetation is the process of replanting and rebuilding the soil of disturbed land. This may be a natural process produced by plant colonization and succession, or an artificial (manmade) wilderness engineering, accelerated process designed to repair damage to a landscape due to wildfire, mining, flood, or other cause.Pith: 250px|right|thumb|[[Elderberry shoot cut longitudinally to show the broad, solid pith (rough-textured, white) inside the wood (smooth, yellow-tinged). Scale in mm.GAI (Arabidopsis thaliana gene)Tropical Asia: Through a crop-based biodiversity, natural resources and animals (birds, fruits, and forests), Tropical Asia is economically and physiogeographically rich. There are 16 countries of Tropical Asia ranging in size from around 610 km² (Singapore) to 3,000,000 km² (India).Aureusidin synthase: Aureusidin synthase (, AmAS1) is an enzyme with system name 2',4,4',6'-tetrahydroxychalcone 4'-O-beta-D-glucoside:oxygen oxidoreductase.Nicotiana glauca: Nicotiana glauca is a species of wild tobacco known by the common name tree tobacco. Its leaves are attached to the stalk by petioles (many other Nicotiana species have sessile leaves), and its leaves and stems are neither [nor sticky like Nicotiana tabacum].The Werewolf (1956 film): The Werewolf is a low-budget American 1956 science fiction horror film, produced by Sam Katzman and directed by Fred F. Sears from a script by Robert E.Stoma: In botany, a stoma (plural "stomata"), also called a stomate (plural "stomates") (from Greek ["mouth"[http://www.perseus.AmborellaTomato seed oil: Tomato seed oil is a vegetable oil extracted from the seeds of tomatoes.List of poisonous plantsPotometerBranching order of bacterial phyla (Gupta, 2001): There are several models of the Branching order of bacterial phyla, one of these was proposed in 2001 by Gupta based on conserved indels or protein, termed "protein signatures", an alternative approach to molecular phylogeny. Some problematic exceptions and conflicts are present to these conserved indels, however, they are in agreement with several groupings of classes and phyla.Sun-dried tomato: Sun-dried tomatoes are ripe tomatoes that lose most of their water content after spending a majority of their drying time in the sun. These tomatoes are usually pre-treated with sulfur dioxide or salt before being placed in the sun in order to improve quality.Flower box: __NOTOC__Opine: Opine biosynthesis is catalyzed by specific enzymes encoded by genes contained in a small segment of DNA (known as the T-DNA, for 'transfer DNA'), which is part of the Ti plasmid, inserted by the bacterium into the plant genome. The opines are used by the bacterium as an important source of nitrogen and energy.Shatter (novel): Shatter is a psychological thriller written by the Australian author Michael Robotham that was published in 2008. Professor Joseph O'Loughlin (referred to as Joe throughout the novel) is tasked by the police with stopping a woman, Christine Wheeler, from committing suicide, only to fail.Protein primary structure: The primary structure of a peptide or protein is the linear sequence of its amino acid structural units, and partly comprises its overall biomolecular structure. By convention, the primary structure of a protein is reported starting from the amino-terminal (N) end to the carboxyl-terminal (C) end.Southern corn leaf blight: Southern corn leaf blight (SCLB) is a fungal disease of maize caused by the plant pathogen Bipolaris maydis (also known as Cochliobolus heterostrophus in its teleomorph state).Chance seedling: A chance seedling is a plant that is the product of unintentional breeding. It may be a genetically unique individual with desirable characteristics that is then intentionally bred.Chemical defense: Chemical defense is the use of chemical compounds by plants and animals to deter herbivory and predation. Chemical defenses can also be used in competitive interactions to prevent overgrowth or maintain spatial dominance.Miljacka Hydroelectric Power Plant: 230px|thumb|right|Miljacka Hydroelectric Power Plant.Lonchocarpus: Lonchocarpus is a plant genus in the legume family (Fabaceae). The species are called lancepods due to their fruit resembling an ornate lance tip or a few beads on a string.Thief of ThievesOctadecanoid pathway: The octadecanoid pathway is a reasonably well-characterized biosynthetic pathway for the production of the phytohormone jasmonic acid (JA), an important hormone for induction of defense genes. JA is synthesized from alpha-linolenic acid, which can be released from the plasma membrane by certain lipase enzymes.Symmetry element: A symmetry element is a point of reference about which symmetry operations can take place. In particular, symmetry elements can be centers of inversion, axes of rotation and mirror planes.Carl Barks: "United States Social Security Death Index," index, FamilySearch (https://familysearch.org/pal:/MM9.Symbiosis Center of Health Care: Symbiosis Center of Health Care (SCHC) is an organization under Symbiosis Society which takes care of health of symbiosis family be it student or staff.http://www.Photosynthesis: Photosynthesis is a process used by plants and other organisms to convert light energy, normally from the Sun, into chemical energy that can be later released to fuel the organisms' activities. This chemical energy is stored in carbohydrate molecules, such as sugars, which are synthesized from carbon dioxide and water – hence the name photosynthesis, from the Greek [phōs, "light", and σύνθεσις], synthesis, "putting together".Auxin binding protein: In molecular biology, the auxin binding protein family is a family of proteins which bind auxin. They are located in the lumen of the endoplasmic reticulum (ER).Superior (potato): 'Superior' is a white-skinned and white-fleshed, midseason potato variety. It was released by the University of Wisconsin potato breeding program in 1962 and is not under plant variety protection.Index of soil-related articles: This is an index of articles relating to soil.Dorjee KhanduEcosystemAlizarine Yellow RJacalin: Jacalin is a plant based lectin, but not a legume lectin, found in jackfruit. It has been studied for capturing O-glycoproteins such as mucins and IgA1, for potential applications in human immunology.Coagulative necrosis: Coagulative necrosis is a type of accidental cell death typically caused by ischemia or infarction. In coagulative necrosis the architecture of dead tissue is preserved for at least a couple of days.Silent mutation: Silent mutations are mutations in DNA that do not significantly alter the phenotype of the organism in which they occur. Silent mutations can occur in non-coding regions (outside of genes or within introns), or they may occur within exons.Arbuscular mycorrhiza: An arbuscular mycorrhizal fungus (plural mycorrhizae or mycorrhizas, a.k.College of Practitioners of PhytotherapyPaddock: A paddock has two primary meanings in different parts of the English-speaking world. In Canada, the USA and UK, a paddock is a small enclosure used to keep horses.LeucoplastCytoplasmic male sterility: Cytoplasmic male sterility is total or partial male sterility in plants as the result of specific nuclear and mitochondrial interactions. Male sterility is the failure of plants to produce functional anthers, pollen, or male gametes.PollenRalph Gretzmacher: Ralph Gretzmacher is an Austrian scientist, professor of botany, zoology and an expert on tropical and subtropic agronomy.Imbibition: Imbibition is a special type of diffusion when water is absorbed by solids-colloids-causing them to enormously increase in volume. The classical examples of imbibition are absorption of water by seeds and dry wood.Tithonia diversifoliaRice bran oilCS-BLASTBreeding for drought stress toleranceHardening (botany): Hardening in botany is the process by which an individual plant becomes tolerant to the effects of freezing during a period of weeks to months. It is a three-stage process.Marine fungi: Marine fungi are species of fungi that live in marine or estuarine environments. They are not a taxonomic group but share a common habitat.Mushy peasPhenotype microarray: The phenotype microarray approach is a technology for high-throughput phenotyping of cells.Wheat middlings: Wheat middlings (also known as millfeed, wheat mill run, or wheat midds) is the middle of three grades into which flour and meal are classified: patents, middlings, and clears. Middlings are often used in animal feed.Phaseic acidPlant stanol ester: Stanol esters are a heterogeneous group of phytosterol esters with a saturated sterol ring structure known to reduce the level of low-density lipoprotein (LDL) cholesterol in blood when ingested. Despite a well documented cholesterol lowering effect, there are no data available indicating that functional foods supplemented with plant sterol esters reduce cardiovascular events.Nitrogen deficiencyLeaf rust (barley): Leaf rust is a fungal disease of barley caused by Puccinia hordei. It is also known as brown rust and it is the most important rust disease on barley.Gamma-150 RNA motif: The gamma-150 RNA motif is a conserved RNA structure that is found in bacteria within the order Pseudomonadales. Because gamma-150 RNAs are not consistently in 5' UTRs, the gamma-150 motif is presumed to correspond to a non-coding RNA.International Moss Stock CenterGrow lightProtoplastHemoglobin E: Hemoglobin E or haemoglobin E (HbE) is an abnormal hemoglobin with a single point mutation in the β chain. At position 26 there is a change in the amino acid, from glutamic acid to lysine.Red chlorophyll catabolite reductase: In molecular biology, the red chlorophyll catabolite reductase (RCC reductase) family of proteins consists of several red chlorophyll catabolite reductase (RCC reductase) proteins. Red chlorophyll catabolite (RCC) reductase (RCCR) and pheophorbide (Pheide) a oxygenase (PaO) catalyse the key reaction of chlorophyll catabolism, porphyrin macrocycle cleavage of Pheide a to a primary fluorescent catabolite (pFCC).Glycine soja: Glycine soja, or wild soybean (previously G. ussuriensis) is an annual plant in the legume family.Mannosylfructose-phosphate synthase: Mannosylfructose-phosphate synthase (, mannosylfructose-6-phosphate synthase, MFPS) is an enzyme with system name GDP-mannose:D-fructose-6-phosphate 2-alpha-D-mannosyltransferase. This enzyme catalyses the following chemical reactionStromule: A stromule is a microscopic structure found in plant cells. Stromules (stroma-filled tubules) are highly dynamic structures extending from the surface of all plastid types, including proplastids, chloroplasts, etioplasts, leucoplasts, amyloplasts, and chromoplasts.Acyrthosiphon pisum: Acyrthosiphon pisum, commonly known as the pea aphid (and colloquially known as the green dolphin, pea louse, and clover louse ), is a sap-sucking insect in the Aphididae family. It feeds on several species of legumes (plant family Fabaceae) worldwide, including forage crops, such as pea, clover, alfalfa, and broad bean, and ranks among the aphid species of major agronomical importance.RhizobiaPeat swamp forest: Peat swamp forests are tropical moist forests where waterlogged soil prevents dead leaves and wood from fully decomposing. Over time, this creates a thick layer of acidic peat.To Kau Wan: To Kau Wan () is a bay on the north shore of northeast Lantau Island, Hong Kong. Contaminated soil from Penny's Bay was transferred here for thermal desorption to separate the Volatile Organic Compounds (VOC) and Semi-Volatile Organic Compounds (SVOC) along with cement immobilization of metal contamination from Penny's Bay.Cytokinin
(1/4690) A single limit dextrinase gene is expressed both in the developing endosperm and in germinated grains of barley.
The single gene encoding limit dextrinase (pullulan 6-glucanohydrolase; EC 126.96.36.199) in barley (Hordeum vulgare) has 26 introns that range in size from 93 to 822 base pairs. The mature polypeptide encoded by the gene has 884 amino acid residues and a calculated molecular mass of 97,417 D. Limit dextrinase mRNA is abundant in gibberellic acid-treated aleurone layers and in germinated grain. Gibberellic acid response elements were found in the promoter region of the gene. These observations suggest that the enzyme participates in starch hydrolysis during endosperm mobilization in germinated grain. The mRNA encoding the enzyme is present at lower levels in the developing endosperm of immature grain, a location consistent with a role for limit dextrinase in starch synthesis. Enzyme activity was also detected in developing grain. The limit dextrinase has a presequence typical of transit peptides that target nascent polypeptides to amyloplasts, but this would not be expected to direct secretion of the mature enzyme from aleurone cells in germinated grain. It remains to be discovered how the enzyme is released from the aleurone and whether another enzyme, possibly of the isoamylase group, might be equally important for starch hydrolysis in germinated grain. (+info)
(2/4690) Characterization of Chlamydomonas reinhardtii zygote-specific cDNAs that encode novel proteins containing ankyrin repeats and WW domains.
Genes that are expressed only in the young zygote are considered to be of great importance in the development of an isogamous green alga, Chlamydomonas reinhardtii. Clones representing the Zys3 gene were isolated from a cDNA library prepared using zygotes at 10 min after fertilization. Sequencing of Zys3 cDNA clones resulted in the isolation of two related molecular species. One of them encoded a protein that contained two kinds of protein-to-protein interaction motifs known as ankyrin repeats and WW domains. The other clone lacked the ankyrin repeats but was otherwise identical. These mRNA species began to accumulate simultaneously in cells beginning 10 min after fertilization, and reached maximum levels at about 4 h, after which time levels decreased markedly. Genomic DNA gel-blot analysis indicated that Zys3 was a single-copy gene. The Zys3 proteins exhibited parallel expression to the Zys3 mRNAs at first, appearing 2 h after mating, and reached maximum levels at more than 6 h, but persisted to at least 1 d. Immunocytochemical analysis revealed their localization in the endoplasmic reticulum, which suggests a role in the morphological changes of the endoplasmic reticulum or in the synthesis and transport of proteins to the Golgi apparatus or related vesicles. (+info)
(3/4690) GTPase activity and biochemical characterization of a recombinant cotton fiber annexin.
A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation. (+info)
(4/4690) A multisubunit acetyl coenzyme A carboxylase from soybean.
A multisubunit form of acetyl coenzyme A (CoA) carboxylase (ACCase) from soybean (Glycine max) was characterized. The enzyme catalyzes the formation of malonyl CoA from acetyl CoA, a rate-limiting step in fatty acid biosynthesis. The four known components that constitute plastid ACCase are biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), and the alpha- and beta-subunits of carboxyltransferase (alpha- and beta-CT). At least three different cDNAs were isolated from germinating soybean seeds that encode BC, two that encode BCCP, and four that encode alpha-CT. Whereas BC, BCCP, and alpha-CT are products of nuclear genes, the DNA that encodes soybean beta-CT is located in chloroplasts. Translation products from cDNAs for BC, BCCP, and alpha-CT were imported into isolated pea (Pisum sativum) chloroplasts and became integrated into ACCase. Edman microsequence analysis of the subunits after import permitted the identification of the amino-terminal sequence of the mature protein after removal of the transit sequences. Antibodies specific for each of the chloroplast ACCase subunits were generated against products from the cDNAs expressed in bacteria. The antibodies permitted components of ACCase to be followed during fractionation of the chloroplast stroma. Even in the presence of 0.5 M KCl, a complex that contained BC plus BCCP emerged from Sephacryl 400 with an apparent molecular mass greater than about 800 kD. A second complex, which contained alpha- and beta-CT, was also recovered from the column, and it had an apparent molecular mass of greater than about 600 kD. By mixing the two complexes together at appropriate ratios, ACCase enzymatic activity was restored. Even higher ACCase activities were recovered by mixing complexes from pea and soybean. The results demonstrate that the active form of ACCase can be reassembled and that it could form a high-molecular-mass complex. (+info)
(5/4690) Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation.
We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398 amino acid residues and is predicted to have six membrane-spanning domains. To obtain evidence for the postulated function of the Arabidopsis gene, transgenic plants with the ZAT coding sequence under control of the cauliflower mosaic virus 35S promoter were analyzed. Plants obtained with ZAT in the sense orientation exhibited enhanced Zn resistance and strongly increased Zn content in the roots under high Zn exposure. Antisense mRNA-producing plants were viable, with a wild-type level of Zn resistance and content, like plants expressing a truncated coding sequence lacking the C-terminal cytoplasmic domain of the protein. The availability of ZAT can lead to a better understanding of the mechanism of Zn homeostasis and resistance in plants. (+info)
(6/4690) The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants.
The Dof proteins are a large family of plant transcription factors that share a single highly conserved zinc finger. The tobacco Dof protein NtBBF1 was identified by its ability to bind to regulatory domain B in the promoter of the rolB oncogene. In this study, we show that the ACT T TA target sequence of NtBBF1 in domain B is necessary for tissue-specific expression of rolB. beta-Glucuronidase (GUS) activity of tobacco plants containing a rolB promoter-GUS fusion with a mutated NtBBF1 target sequence within domain B is almost completely suppressed in apical meristems and is severely abated in the vascular system. The ACT T TA motif is shown here also to be one of the cis-regulatory elements involved in auxin induction of rolB. The pattern of NtBBF1 expression in plants is remarkably similar to that of rolB, except in mesophyll cells of mature leaves, in which only NtBBF1 expression could be detected. Ectopic expression of rolB in mesophyll cells was achieved by particle gun delivery if the NtBBF1 binding sequence was intact. These data provide evidence that in the plant, a Dof protein DNA binding sequence acts as a transcriptional regulatory motif, and they point to NtBBF1 as the protein involved in mediating tissue-specific and auxin-inducible expression of rolB. (+info)
(7/4690) Isolation and characterization of SYN1, a RAD21-like gene essential for meiosis in Arabidopsis.
The proper pairing, recombination, and segregation of chromosomes are central to meiosis and sexual reproduction. The syn1 mutation was previously identified as a synaptic mutant in a T-DNA-tagged population of plants. SYN1 has been isolated and found to exhibit similarity to Schizosaccharomyces pombe RAD21 and RAD21-like proteins, which are required for chromosome condensation and sister chromatid cohesion during mitosis. Plants homozygous for syn1 are male and female sterile and show defects in chromosome condensation and pairing beginning at leptonema of meiosis I. Fragmentation of the chromosomes was observed at metaphase I. Alternative promoters produced two SYN1 transcripts. One transcript was expressed at low levels in most tissues, whereas the other was expressed only in prebolting buds. DNA blot analyses suggest that Arabidopsis contains a small RAD21 gene family. Consistent with the DNA blot data, a second Arabidopsis RAD21-like gene has been identified. These results suggest that different RAD21-like proteins play essential roles in chromosome condensation and pairing during both meiosis and mitosis. (+info)
(8/4690) The FLF MADS box gene: a repressor of flowering in Arabidopsis regulated by vernalization and methylation.
A MADS box gene, FLF (for FLOWERING LOCUS F ), isolated from a late-flowering, T-DNA-tagged Arabidopsis mutant, is a semidominant gene encoding a repressor of flowering. The FLF gene appears to integrate the vernalization-dependent and autonomous flowering pathways because its expression is regulated by genes in both pathways. The level of FLF mRNA is downregulated by vernalization and by a decrease in genomic DNA methylation, which is consistent with our previous suggestion that vernalization acts to induce flowering through changes in gene activity that are mediated through a reduction in DNA methylation. The flf-1 mutant requires a greater than normal amount of an exogenous gibberellin (GA3) to decrease flowering time compared with the wild type or with vernalization-responsive late-flowering mutants, suggesting that the FLF gene product may block the promotion of flowering by GAs. FLF maps to a region on chromosome 5 near the FLOWERING LOCUS C gene, which is a semidominant repressor of flowering in late-flowering ecotypes of Arabidopsis. (+info)
- I have tried the CTAB method as usually done for plant tissues eg. (bio.net)
- NucleoMag 96 Plant is designed for the rapid parallel purification of genomic DNA from plant tissues in a 96-well format. (clontech.com)
- NucleoSpin Plant II is a next-generation kit designed for the rapid isolation of genomic DNA from plant cells and tissue, with higher yield and quality. (clontech.com)
- 20 mg plant tissue (dry weight) with yields typically ranging from 1-30 µg genomic DNA. (clontech.com)
- The NucleoSpin Plant Midi and Plant Maxi Kits are designed for isolating genomic DNA from medium to large samples of plant tissue. (clontech.com)
- After lysis of the sample, genomic DNA is selectively bound to the NucleoMag Beads. (clontech.com)
- Excising this so-called "genomic island" eliminates production of the bacterial protein detected by the plant and allows a more stealthy - and successful - invasion. (innovations-report.com)
- But this genomic island also encodes enzymes that, when switched on, snip the DNA on either side of the island, resulting in the excision of the entire island from the genome. (innovations-report.com)
- If some bacterial proteins give away the presence of the pathogen to the plant, why are they and their surrounding genomic islands maintained by the bacteria at all? (innovations-report.com)
- Remarkably, bacteria that have excised the genomic island do not appear compromised in their ability to grow and cause disease within the host bean plant. (innovations-report.com)
- Could anybody please help me with DNA isolation from plant culture cells? (bio.net)
- Eukaryotic organisms ( animals , plants , fungi , and protists ) store most of their DNA inside the cell nucleus and some of their DNA in organelles , such as mitochondria or chloroplasts . (wikipedia.org)
- Seeking to catch an arms-race maneuver in action, researchers have uncovered new evidence to explain how bacteria in the process of infecting a plant can shift molecular gears by excising specific genes from its genome to overcome the host plant s specific defenses. (innovations-report.com)
- Throughout evolution - in the wild and in crops cultivated by humans - plants have developed systems for resisting the attack of microbial pathogens, while these microbes themselves have depended on their ability to alter molecular attack strategies in order to flourish. (innovations-report.com)
- In the new work, researchers have essentially caught one step of this arms race in action, and they have shed light on the molecular mechanisms employed by a bacterial pathogen to survive in the face of its host plant s defenses. (innovations-report.com)
- These findings offer a molecular explanation for how exposure to plant resistance mechanisms can directly drive the evolution of new virulent forms of a bacterial pathogen. (innovations-report.com)
- This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. (springer.com)
- With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits. (springer.com)
- DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity . (wikipedia.org)
- During cell division these chromosomes are duplicated in the process of DNA replication , providing each cell its own complete set of chromosomes. (wikipedia.org)
- I have extracted DNA from Leaves and fruits and around 95% of the sequences i obtained were from the choroplast and mitochondria from the plant, and actually i am interested in the bacteria growing in this samples. (protocol-online.org)
- Since plants are very heterogenous and contain many different metabolites, such as polyphenols, polysaccharides, or acidic components, NucleoSpin Plant II offers two different lysis procedures for optimal processing of various samples. (clontech.com)
- The role of repetitive DNA in structure and evolution of sex chromosomes in plants. (biomedsearch.com)
- Here we review the occurrence and role of repetitive DNA in Y chromosome evolution in various species with a focus on dioecious plants. (biomedsearch.com)
- Studying interactions between strains of the halo-bright pathogen and bean plants, the researchers found that the pathogenic bacteria essentially kicks out a section of its genome when it senses that its presence has been detected by the plant s defense system. (innovations-report.com)
- does someone know a protocol to separate the plant protoplast from the bacteria to use it before performing the DNA extraction? (protocol-online.org)
-  In contrast prokaryotes ( bacteria and archaea ) store their DNA only in the cytoplasm . (wikipedia.org)
- The crop-destroying gray mold fungus uses RNA weapons to disable plant defenses and invade. (the-scientist.com)
- Researchers identify a structure in plants responsible for the production of tannins. (the-scientist.com)
- Researchers examine how underground microbes and nutrients affect plant populations. (the-scientist.com)
- Our results suggest that RdDM in plants is largely guided by a previously unappreciated class of Dicer-independent non-coding RNAs, and that siRNAs are required to maintain DNA methylation at only a subset of loci. (nature.com)
- The reason the strategy can be successful is that the plant has evolved to recognize the presence of only certain bacterial proteins as warnings of an infection. (innovations-report.com)
- Within the eukaryotic chromosomes, chromatin proteins such as histones compact and organize DNA. (wikipedia.org)
- These compact structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed. (wikipedia.org)
- Plants and algae use the enzyme Rubisco to fix carbon dioxide, removing it from the atmosphere and converting it into biomass. (innovations-report.com)
- In their study, the authors identify within the halo-bright pathogen genome a special island of DNA that encodes one such offending protein. (innovations-report.com)
- Eukaryotic genomes contain a large proportion of repetitive DNA sequences, mostly transposable elements (TEs) and tandem repeats. (biomedsearch.com)
- Within eukaryotic cells DNA is organized into long structures called chromosomes . (wikipedia.org)
- Small interfering RNAs (siRNAs) generated by Dicers from RNA polymerase IV (Pol IV) transcripts are thought to guide sequence-specific DNA methylation. (nature.com)
- DNA methylation in plants occurs in three different sequence contexts, CG, CHG and CHH (where H is A, C or T), each maintained by different DNA methyltransferases and pathways. (nature.com)
- Second, when you use any similar procedure with plant LEAVES, the grinding in LN2 must NOT be omitted. (bio.net)
- A large part of DNA (more than 98% for humans) is non-coding , meaning that these sections do not serve as patterns for protein sequences. (wikipedia.org)
- I don't know much about it so can any one tell me about DNA as well as RNA Extraction in detail. (protocol-online.org)
- Instead, DNA methylation levels correlated with the accumulation of Pol IV-dependent 25-50 nt RNAs at most loci in Dicer mutant plants. (nature.com)
- The two DNA strands are called polynucleotides since they are composed of simpler monomer units called nucleotides . (wikipedia.org)
- DNA is a long polymer made from repeating units called nucleotides . (wikipedia.org)
- The asymmetric CHH methylation is maintained by two different DNA methyltransferases, CMT2 and DRM2. (nature.com)
- Pitcher plants across different continents acquired their tastes for meat in similar ways. (the-scientist.com)
- The structure of the DNA double helix . (wikipedia.org)
- The DNA backbone is resistant to cleavage , and both strands of the double-stranded structure store the same biological information. (wikipedia.org)
-   The structure of DNA is dynamic along its length, being capable of coiling into tight loops, and other shapes. (wikipedia.org)
- In non-recombining regions of the Y chromosome repetitive DNA sequences are accumulated, representing a dominant and early process forming the Y chromosome, probably before genes start to degenerate. (biomedsearch.com)
- RNA strands are created using DNA strands as a template in a process called transcription . (wikipedia.org)
- Scientists discover fossilized remnants of a flowering plant about 100 million years older than the oldest previously found. (the-scientist.com)
- The unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. (wikipedia.org)
- Following natural disasters or violent political turmoil, DNA science can help identify victims. (the-scientist.com)
- Today's tulip trees carry similar mitochondrial DNA as those that grew in the time of the dinosaurs. (the-scientist.com)
- Two papers on plant immunity have been retracted, and questions remain about others with similar results. (the-scientist.com)
- The silica membrane in the NucleoSpin Plant II columns is optimized to improve DNA binding. (clontech.com)
- Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. (clontech.com)
- Most DNA molecules consist of two biopolymer strands coiled around each other to form a double helix . (wikipedia.org)
- For other uses, see DNA (disambiguation) . (wikipedia.org)
- Typical yields range from 20 to 80 µg of DNA for NucleoSpin Plant Midi, and 60 to 260 µg of DNA for NucleoSpin Plant Maxi, depending on the size and source of the sample. (clontech.com)
- The nitrogenous bases of the two separate polynucleotide strands are bound together, according to base pairing rules (A with T, and C with G), with hydrogen bonds to make double-stranded DNA. (wikipedia.org)