No data available that match "DNA, Fungal"
Yeast and Fungal DNA Page 5View genomic DNA products from ATCC fungi and yeast cultures ... Yeast and Fungal DNA Nucleic Acids. Save time and money with ...
Yeast and Fungal DNA Page 1View genomic DNA products from ATCC fungi and yeast cultures ... Yeast and Fungal DNA * Vanderwaltozyma polyspora (van der Walt ... Designation: Genomic DNA from Vanderwaltozyma polyspora strain CBS 2163 (ATCC® 22028™) Product Format: freeze-dried Total DNA: ... Genomic DNA is appropriate for PCR* and other molecular biology applications.. *The polymerase chain reaction (PCR) process is ...
Yeast and Fungal DNA Page 1View genomic DNA products from ATCC fungi and yeast cultures ... Yeast and Fungal DNA * Zygosaccharomyces rouxii (Boutroux) ... This preparation of high molecular weight DNA is appropriae for use in the polymerase chain reaction (PCR)* process and other ...
The Polymerase Chain Reaction (PCR) Analysis of Nasal Polyps for Fungal DNA - Full Text View - ClinicalTrials.govThe Polymerase Chain Reaction (PCR) Analysis of Nasal Polyps for Fungal DNA. The recruitment status of this study is unknown. ... The objective of this study is to determine whether the amount or type of fungal DNA present in the nose and home environment ... The objective of this study is to determine whether the amount or type of fungal DNA present in the nose and home environment ... The hypothesis is that the quantity and type of fungal DNA present in the nose and home environment are directly correlated ...
Fungal and Yeast Genomic DNA Page 1ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains. ... Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ... Fungal and Yeast Genomic DNA Fast STR Profiling Service Authentication of cell lines via STR profile analysis is becoming a ...
Fungal and Yeast Genomic DNA Page 1ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains. ... Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ... Fungal and Yeast Genomic DNA * Lachancea kluyveri (Phaff et al.) Kurtzman (ATCC® 58438D-5™) ATCC® Number: 58438D-5™ Deposited ... Designation: Genomic DNA from strain UCD 72-13 (ATCC® 56500™) Product Format: freeze-dried Total DNA: Approximately 5 µg in 1X ...
Fungal and Yeast Genomic DNA Page 1ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains. ... Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ... Fungal and Yeast Genomic DNA * Acremonium alcalophilum Okada (ATCC® 90507D-2™) ATCC® Number: 90507D-2™ Designation: genomic DNA ... Designation: Genomic DNA from Aspergillus brasiliensis strain SN 26 [ATCC® 9642™] Product Format: freeze-dried Total DNA: ...
3/2017 | Fungal Model to Shed New Light on Human DNA | Amherst CollegeFungal Model to Shed New Light on Human DNA. The National Institutes of Health has awarded Professor Michael Hood a $444,651 ... Fungal Model to Shed New Light on Human DNA. ... There are other advantages to anther smut as well: the fungal ... The problem lies in the unusual nature of the human male sex chromosomes, the only two on the long string of human DNA that are ... It's a question that has baffled scientists since they began understanding DNA, and is one that Michael E. Hood, associate ...
'group homes' Protocols and Video...Air-sampled Filter Analysis for Endotoxins and DNA Content', 'Electroactive Polymer Nanoparticles Exhibiting Photothermal ... Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA', 'Standardized Method for High-throughput ... Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA. Angela R. Lemons1, William G. Lindsley1, ... Air-sampled Filter Analysis for Endotoxins and DNA Content. Naama Lang-Yona1,2, Yinon Mazar1, Michal Pardo1, Yinon Rudich1 ...
Yeast diversity in the acidic Rio Agrio-Lake Caviahue volcanic environment (Patagonia, Argentina).DNA, Fungal / genetics. Fresh Water / microbiology. Hydrogen-Ion Concentration. Microbial Viability. Phylogeny. Rhodotorula / ...
NoviPure Soil Protein Kit - QIAGEN Online ShopAllPrep Fungal DNA/RNA/Protein Kit (50) For the extraction of protein and nucleic acids from fungal cell cultures ... RNeasy PowerSoil DNA Elution Kit (25) For co-isolation of DNA and RNA from soil using the RNA PowerSoil Total RNA Isolation Kit ... AllPrep Bacterial DNA/RNA/Protein Kit (50) For the extraction of protein and nucleic acids from bacterial cell cultures ...
Candice Joy McNeil, M.D., M.P.H., Infectious Diseases - Wake Forest Baptist, North CarolinaAscomycota; Brain Abscess; Mycoses; DNA, Ribosomal; DNA, Fungal More » Contact Information. Academic: 336-716-2700 , Department ...
When Do We Call Genetically Distinct Strains Different Species? - A Cautionary Case Study of the Colletotrichum gloesporioides...Due to the increasing availability of DNA sequence information, the phylogenetic species concept (PSC) has bec.. ... Different criteria have been used to define fungal species. ... Fungal Genet Biol 31: 21-32.. *Xu J (2016) Fungal DNA barcoding ... In a recent study, we reported the DNA sequences of four gene loci for each of 199 strains of the fungal pathogen ... Different criteria have been used to define fungal species. Due to the increasing availability of DNA sequence information, the ...
Extensive domain shuffling in transcription regulators of DNA viruses and implications for the origin of fungal APSES...The KilA-N domain is suggested to be homologous to the fungal DNA-binding APSES domain. We provide evidence for the KilA-N and ... Extensive domain shuffling in transcription regulators of DNA viruses and implications for the origin of fungal APSES ... We show here that KilA-N and Bro-N domains are two DNA-binding domains that are widespread in large DNA viruses infecting ... Thus, given that the Bro-N domain is a DNA-binding domain, it appears plausible that it adopts a fold similar to that of the ...
Laboratory InterpretationBacterial or fungal DNA in blood or valve (including Bartonella sp., Tropheryma whipplei, other new or unusual organisms) ,/li ... Fungal infections (Blastomyces, Coccidioides, Candida, Aspergillus, Zygomycetes, Cladosporium, Allescheria) ,/li,,/ul,,ul,,li, ... Fungal infections (e.g., Cryptococcus neoformans, Candida species, Coccidioides immitis, Histoplasma capsulatum, Blastomyces ...
Molecular technique advances soybean rust resistanc... ( A new tool is available to select fo...)However, Q-PCR allows for exact enumeration of fungal DNA in the tissue. This is particularly helpful when plants show similar ... but colonization levels vary based on fungal DNA levels.. "The eye can easily tell us if it's a plus or minus for qualitative ... assays to assess fungal DNA in soybean leaf tissue to quantify the level of resistance in individual plants with resistance to ... Combined molecular study techniques reveal more about DNA proteins. 11. Fingerprints match molecular simulations with reality. ...
plant science | Biology LettersPaternal arbuscular mycorrhizal fungal status affects DNA methylation in seeds. Sandra Varga, Carl D. Soulsbury ...
QuantiFast Pathogen +IC Kits - QIAGENFor sensitive and reliable detection of viral RNA/DNA and bacterial DNA, including internal control ... or fungal DNA, which contains an internal DNA control template, plus the Internal Control primer/probe set. With both kits, ROX ... Pathogen Detection: Real-time PCR of viral, bacterial or fungal DNA (QuantiFast Pathogen PCR +IC Kit) or one-step RT-PCR for ... Internal Control DNA template in the QuantiFast Pathogen PCR +IC Kit. A universal DNA amplification control that can be used ...
Helping Africa researchers improve crops, susta...Predicting Take-All Severity in Second-Year Wheat Using Soil DNA Concentrations of Fungal Causal Agens ... tritici DNA-based take-all prediction system developed in Australia, with four take-all risk categories. These categories were ... 2012) published results of DNA-based take-all prediction of the risk of wheat disease. This information could improve ... 2012) Predicting Take-All Severity in Second-Year Wheat Using Soil DNA Concentrations of Gaeumannomyces graminis var. tritici ...
Region definition procedure and creation of a repeat sequence file - PERSON CHRISTOPHEwherein said sequences are derived from fungal DNA or RNA. 13. The method of claim 1 or 2. , wherein said sequences are derived ... Redundancies in the currently available DNA/RNA databases render the systematic analysis of similarity or homology between DNA/ ... wherein said sequences are derived from DNA or RNA of a single-cell eukaryote. 15. The method of claim 1 or 2. , wherein said ... wherein said sequences are derived from animal DNA or RNA. 8. The method of claim 7, wherein said animal is a human. 9. The ...
Clinical Utility of the Cryptococcal Antigen Lateral Flow Assay in a Diagnostic Mycology Laboratory2007) Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J ... Molecular diagnosis of C. neoformans complex was by PCR amplification and DNA sequencing of the fungal internal transcribed ...
Jan SuchodolskiPrevalence and identification of fungal DNA in the small intestine of healthy dogs and dogs with chronic enteropathies. ... Fungal microbiome of healthy and allergic canine skin - a next-generation sequencing study Smith CM, Diesel A, Patterson AP, ... Characterization of the fungal microbiome (mycobiome) in fecal samples from dogs. Foster ML, Dowd SE, Stephenson C, Steiner JM ... Panfungal PCR for identification of fungal pathogens in formalin-fixed animal tissues Meason-Smith C, Edwards E, Older CE, ...
"Nonsense-mediated mRNA decay in yeast" by Stuart W. Peltz, Feng He et al.Base Sequence; Codon; DNA, Fungal; Molecular Sequence Data; RNA, Fungal; RNA, Messenger; Regulatory Sequences, Nucleic Acid; ...
Adelaide Research & Scholarship: Ulocladium atrum keratitisHumans; Ascomycota; Eye Infections, Fungal; Keratitis; DNA, Fungal; Base Sequence; Genes, Fungal; Molecular Sequence Data; ...
No data available that match "DNA, Fungal"
(1/8779) The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae.
Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5'-->3' Exo1 and the 3'-->5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) mutants defective in 3'-->5' proofreading exonuclease, mutants defective in the 5'-->3' exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes. (+info)
(2/8779) Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae.
Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex. (+info)
(3/8779) Histone octamer transfer by a chromatin-remodeling complex.
RSC, an abundant, essential chromatin-remodeling complex related to SWI/SNF complex, catalyzes the transfer of a histone octamer from a nucleosome core particle to naked DNA. The newly formed octamer-DNA complex is identical with a nucleosome in all respects. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. The mechanism may entail formation of a duplex displacement loop on the nucleosome, facilitating the entry of exogeneous DNA and the release of the endogenous molecule. (+info)
(4/8779) Regulation of the start of DNA replication in Schizosaccharomyces pombe.
Cells of Schizosaccharomyces pombe were grown in minimal medium with different nitrogen sources under steady-state conditions, with doubling times ranging from 2.5 to 14 hours. Flow cytometry and fluorescence microscopy confirmed earlier findings that at rapid growth rates, the G1 phase was short and cell separation occurred at the end of S phase. For some nitrogen sources, the growth rate was greatly decreased, the G1 phase occupied 30-50% of the cell cycle, and cell separation occurred in early G1. In contrast, other nitrogen sources supported low growth rates without any significant increase in G1 duration. The method described allows manipulation of the length of G1 and the relative cell cycle position of S phase in wild-type cells. Cell mass was measured by flow cytometry as scattered light and as protein-associated fluorescence. The extensions of G1 were not related to cell mass at entry into S phase. Our data do not support the hypothesis that the cells must reach a certain fixed, critical mass before entry into S. We suggest that cell mass at the G1/S transition point is variable and determined by a set of molecular parameters. In the present experiments, these parameters were influenced by the different nitrogen sources in a way that was independent of the actual growth rate. (+info)
(5/8779) The prokaryotic beta-recombinase catalyzes site-specific recombination in mammalian cells.
The development of new strategies for the in vivo modification of eukaryotic genomes has become an important objective of current research. Site-specific recombination has proven useful, as it allows controlled manipulation of murine, plant, and yeast genomes. Here we provide the first evidence that the prokaryotic site-specific recombinase (beta-recombinase), which catalyzes only intramolecular recombination, is active in eukaryotic environments. beta-Recombinase, encoded by the beta gene of the Gram-positive broad host range plasmid pSM19035, has been functionally expressed in eukaryotic cell lines, demonstrating high avidity for the nuclear compartment and forming a clear speckled pattern when assayed by indirect immunofluorescence. In simian COS-1 cells, transient beta-recombinase expression promoted deletion of a DNA fragment lying between two directly oriented specific recognition/crossing over sequences (six sites) located as an extrachromosomal DNA substrate. The same result was obtained in a recombination-dependent lacZ activation system tested in a cell line that stably expresses the beta-recombinase protein. In stable NIH/3T3 clones bearing different number of copies of the target sequences integrated at distinct chromosomal locations, transient beta-recombinase expression also promoted deletion of the intervening DNA, independently of the insertion position of the target sequences. The utility of this new recombination tool for the manipulation of eukaryotic genomes, used either alone or in combination with the other recombination systems currently in use, is discussed. (+info)
(6/8779) The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance.
The mdm17 mutation causes temperature-dependent defects in mitochondrial inheritance, mitochondrial morphology, and the maintenance of mitochondrial DNA in the yeast Saccharomyces cerevisiae. Defects in mitochondrial transmission to daughter buds and changes in mitochondrial morphology were apparent within 30 min after shifting cells to 37 degrees C, while loss of the mitochondrial genome occurred after 4-24 h at the elevated temperature. The mdm17 lesion mapped to MGM1, a gene encoding a dynamin-like GTPase previously implicated in mitochondrial genome maintenance, and the cloned MGM1 gene complements all of the mdm17 mutant phenotypes. Cells with an mgm1-null mutation displayed aberrant mitochondrial inheritance and morphology. A version of mgm1 mutated in a conserved residue in the putative GTP-binding site was unable to complement any of the mutant defects. It also caused aberrant mitochondrial distribution and morphology when expressed at high levels in cells that also contained a wild-type copy of the gene. Mgm1p was localized to the mitochondrial outer membrane and fractionated as a component of a high molecular weight complex. These results indicate that Mgm1p is a mitochondrial inheritance and morphology component that functions on the mitochondrial surface. (+info)
(7/8779) All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature.
All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned. These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature. The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs. Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex. (+info)
(8/8779) The nuclear ribosomal DNA intergenic spacer as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on pinus root systems.
Polymorphism of the nuclear ribosomal DNA intergenic spacer (IGS) of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was studied to evaluate whether this sequence could be used in field studies to estimate the diversity of strains forming mycorrhizas on individual Pinus pinaster root systems. This sequence was amplified by PCR from 125 haploid homokaryotic strains collected in 14 P. pinaster stands along the Atlantic coast of France by using conserved oligonucleotide primers. Restriction enzyme digestion of the amplified 3.4-kbp-long IGS allowed us to characterize 24 alleles whose frequencies differed. Nine of these alleles were found only once, whereas about 60% of the strains contained four of the alleles. Local populations could be almost as diverse as the entire population along a 150-km stretch of coastline that was examined; for example, 13 alleles were found in a single forest stand. The IGS from one strain was partially sequenced, and the sequence data were used to design oligonucleotides which allowed separate PCR amplification of three different segments of the IGS. Most polymorphisms observed among the full-length IGS regions resulted from polymorphisms in an internal ca. 1,500-bp-long sequence characterized by length variations that may have resulted from variable numbers of a T2AG3 motif. This internal polymorphic sequence could not be amplified from the genomes of nine other Hebeloma species. Analysis of this internal sequence amplified from the haploid progenies of 10 fruiting bodies collected in a 70-m2 area resulted in identification of six allelic forms and seven distinct diplotypes out of the 21 possible different combinations. Moreover, optimization of the PCR conditions resulted in amplification of this sequence from more than 80% of the DNA samples extracted from individual H. cylindrosporum infected P. pinaster mycorrhizal root tips, thus demonstrating the usefulness of this sequence for studying the below-ground diversity of mycorrhizas formed by genets belonging to the same fungal species. (+info)
- Genomic DNA is appropriate for PCR* and other molecular biology applications. (atcc.org)
- Genomic DNA from Genome sequencing strain (Genolevures Consortium, France). (atcc.org)
- Genomic DNA from Genome sequencing strain (Broad Institute, USA). (atcc.org)
- Is the Subject Area "Fungal genomics" applicable to this article? (plos.org)
invasive fungal infections
- The major problem in managing life threatening invasive fungal infections in patients (pts) with acute leukemia and pts after allogeneic hematopoietic stem cell transplantation is the lack of sensitive and specific diagnostic tools to identify fungal pathogens reliably and early in the course of the disease. (knowcancer.com)
- No obvious clinical symptoms distinguish invasive fungal infections from other microbial infections. (frontiersin.org)
- Using computational analysis, we show that the amino-terminal module of the D6R/N1R proteins defines a novel, conserved DNA-binding domain (the KilA-N domain) that is found in a wide range of proteins of large bacterial and eukaryotic DNA viruses. (biomedcentral.com)
- Two kit formats are available: The QuantiFast Pathogen RT-PCR +IC Kit for detection of viral RNA, which contains an internal RNA control template, plus the Internal Control primer/probe set, or the QuantiFast Pathogen PCR +IC Kit for detection of viral, bacterial, or fungal DNA, which contains an internal DNA control template, plus the Internal Control primer/probe set. (qiagen.com)
- Ancient DNA - DNA obtained from fossils up to about 100,000 years old - is highly fragmented, is present in only trace amounts, and is usually swamped by bacterial and fungal DNA. (nature.com)
- If hair still contains DNA, almost all of it will belong to the extinct species, and will not be of bacterial or fungal origin, as is often the case with bones. (nature.com)
- In a recent study, we reported the DNA sequences of four gene loci for each of 199 strains of the fungal pathogen Colletotrichum gloeosporioides species complex (CGSC) causing leaf anthracnose on the tea-oil tree Camellia oleifera in southern China. (omicsonline.org)
- Toll-like receptors (TLRs) are essential players in this balance, due to their ability to control both inflammatory and anti-inflammatory processes upon recognition of fungal-specific pathogen-associated molecular patterns (PAMPs). (frontiersin.org)
- representing the major human fungal pathogen ( Del Poeta and Chaturvedi, 2012 ). (frontiersin.org)
- Aspergillus, Cryptoccocus , and Coccidoides ), as well as to the most frequent human fungal pathogens represented by the commensal Candida species. (frontiersin.org)
- While only a few fungal microbes are actually true pathogens for healthy individuals, in Western societies opportunistic fungi can cause life-threatening infections in immunosuppressed individuals, ranging from superficial mucocutaneous disease to invasive deep-seated infections. (frontiersin.org)
- The main fungal pathogens affecting humans comprise those ubiquitously present in the environmental fungi, Aspergillus fumigatus , Cryptoccocus neoformans and more recently Cryptoccocus gatii , Histoplasma capsulatum , Coccidoides posadasii, Pneumocystis jirovecii and the commensal P. jiroveci or the Candida spp. (frontiersin.org)
- Hartman and his team of researchers successfully used quantitative polymerase chain reaction (Q-PCR) assays to assess fungal DNA in soybean leaf tissue to quantify the level of resistance in individual plants with resistance to soybean rust. (bio-medicine.org)
- Additionally, if genomic material of Aspergillus fumigatus is detected by PCR in a clinical sample, we investigate fungal DNA for point mutations in the cyp51A gene mediating resistance against common mould-active triazoles with novel rapid, sensitive and specific, non-culture-based PCR-assays and sequencing to optimize antifungal treatment as early as possible. (knowcancer.com)
- Fungal Genom Biol 6:146. (omicsonline.org)
- The Observer Sean Eddy (2012) The C-value paradox, junk DNA, and ENCODE, Curr Biol 22(21):R898-R899. (wikipedia.org)
- Results of other diagnostic means including culture findings as well as patients` clinical data (e.g. duration of neutropenia, underlying disease and outcome including follow-up data concerning antifungal treatment and mortality attributable to fungal infections) will be documented, pseudonomyzed and included in our data bank. (knowcancer.com)
- Decrease in host immune fitness or increase in fungal burden may favor pathologies, ranging from superficial mucocutaneous diseases to invasive life-threatening fungal infections. (frontiersin.org)
- The spatio-temporal and physiological contributions of individual TLRs in fungal infections remains ill-defined, although in humans, TLR gene polymorphisms have been linked to increased susceptibility to fungal infections. (frontiersin.org)
- The rising incidence in fungal infections observed in the last decades correlates with increases in invasive medical interventions, long-term hospitalization and with large numbers of immunosuppressed patients due to acquired- (e.g. (frontiersin.org)
polymerase chain re
- This preparation of high molecular weight DNA is appropriae for use in the polymerase chain reaction (PCR)* process and other molecular biology applications. (atcc.org)
- This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications. (atcc.org)
- Different criteria have been used to define fungal species. (omicsonline.org)
- Due to the increasing availability of DNA sequence information, the phylogenetic species concept (PSC) has become popular among fungal taxonomists. (omicsonline.org)
- Thus, while the biological species concept is the dominant species concept for animals and plants, it has not been used broadly to define fungal species. (omicsonline.org)
- BACKGROUND Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible to culture. (fiocruz.br)
- OBJECTIVE In this study, we aimed to standardise an in situ hybridisation technique for the differentiation between the pathogenic species Paracoccidioides brasiliensis and Paracoccidioides lutzii , by using species-specific DNA probes targeting the internal transcribed spacer -1 (ITS-1) of the rRNA gene. (fiocruz.br)
- Hundred-thousands of fungal species are present in our environment, including normal colonizers that constitute part of the human microbiota. (frontiersin.org)
- An estimated 1.5 million fungal species are present in the environment ( Hube, 2009 ). (frontiersin.org)
- Both species may occupy the same host within the same environment, but are nevertheless phylogenetically distinct, as inferred from analyses of both mitochondrial and nuclear DNA sequences. (ingentaconnect.com)
- The homeostasis of host-fungus interactions encompasses efficient fungal sensing, tolerance at mucosal surfaces, as well as antifungal defenses. (frontiersin.org)
- It's a difficult question to explore since the answer involves tracking human DNA and the harmful mutations genomes accumulate over long periods of time. (amherst.edu)
- There are other advantages to anther smut as well: the fungal models are safe for researchers to use, the fungus is easy to grow and, unlike humans, fungi have small genomes that make the sequencing process simple. (amherst.edu)
- Computational analysis of viral DNA-binding regulatory proteins and identification of their previously undetected homologs encoded by cellular genomes might lead to a better understanding of their function and evolution in both viral and cellular systems. (biomedcentral.com)
- A detailed analysis of the KilA-N and Bro-N domains and the associated domains points to extensive domain shuffling and lineage-specific gene family expansion within DNA virus genomes. (biomedcentral.com)
- This is an ideal setting for preserving DNA, and, moreover, for preserving hair, which is an ideal source of DNA for sequencing ancient genomes. (nature.com)
- Attaching DNA to a modified glass surface is a central step for many applications in DNA diagnostics industry including gene expression analysis. (google.com)
- 2006), but in cases of few infective fungal cells or uncultivable material, molecular techniques such as in situ detection of specific target DNA could be a promising choice (Perlin & Zhao 2009, Ampel 2010, Koepsell et al. (fiocruz.br)
- Total DNA: Approximately 5 µg in 1X Tris buffer, then dried. (atcc.org)
- Clinical samples (BAL and blood) from approximately 100 pts suffering from acute leukemia and pts after allogeneic stem cell transplantation with febrile neutropenia and lung infiltrates diagnosed in a chest CT scan suggestive for fungal infection will be investigated after pts`s informed consent in a multicentre, prospective trial. (knowcancer.com)
- Viral DNA-binding proteins have served as good models to study the biochemistry of transcription regulation and chromatin dynamics. (biomedcentral.com)
- The phyletic range and the conserved DNA-binding domains of the viral regulatory proteins of the poxvirus D6R/N1R and baculoviral Bro protein families have not been previously defined. (biomedcentral.com)
- The amino-terminal module of the Bro proteins is another, distinct DNA-binding domain (the Bro-N domain) that is present in proteins whose domain architectures parallel those of the KilA-N domain-containing proteins. (biomedcentral.com)
- We define a large class of novel viral DNA-binding proteins and their cellular homologs and identify their domain architectures. (biomedcentral.com)
- Much less is known of the domain architecture and evolutionary history of those viral DNA-binding regulatory proteins whose cellular homologs have not (yet) been identified. (biomedcentral.com)
- Is the Subject Area "DNA-binding proteins" applicable to this article? (plos.org)
- On the basis of phyletic pattern analysis we present evidence for a probable viral origin of the fungus-specific cell-cycle regulatory transcription factors containing the APSES DNA-binding domain. (biomedcentral.com)
- Several viral DNA-binding regulators have conserved domains that are shared with cellular transcription factors. (biomedcentral.com)
- It's a question that has baffled scientists since they began understanding DNA, and is one that Michael E. Hood, associate professor of biology, believes he can help answer-using a violet fungus that infects a tiny carnation-like flower. (amherst.edu)
- This is particularly helpful when plants show similar visual symptoms, but colonization levels vary based on fungal DNA levels. (bio-medicine.org)
- Thus, anti-fungal treatments are often delayed or inappropriately applied. (frontiersin.org)
- Thus, the authors needed to sequence a total of 'only' 4.1 billion base pairs to obtain about 3.3 billion base pairs of mammoth DNA. (nature.com)
- Although the mammoth genome is larger than the human genome, the DNA substitution rate seems to be smaller - this is the rate at which one nucleotide replaces another, and so is a measure of evolutionary change. (nature.com)
- Certain members of the TLR family participate to the initial recognition of fungal PAMPs on the cell surface, as well as inside phagosomes of innate immune cells. (frontiersin.org)
- In general, DNA can be attached to a glass surface either through non-covalent, ionic interactions, or through multi-step processes or simple coupling reactions. (google.com)
- The journal publishes high-quality papers elucidating known and novel fungal taxa at the DNA level, and also strives to present novel insights into evolutionary processes and relationships. (ingentaconnect.com)
- Large eukaryotic DNA viruses, such as poxviruses, phycodnaviruses, phaeoviruses, asfarviruses, iridoviruses (all of which form the recently identified monophyletic clade of nucleo-cytoplasmic large DNA viruses (NCLDVs) [ 9 ]), baculoviruses and herpesviruses, also encode a number of transcription factors. (biomedcentral.com)
- W hy is the Y chromosome, which determines male development, slowly disappearing in human DNA? (amherst.edu)
- The problem lies in the unusual nature of the human male sex chromosomes, the only two on the long string of human DNA that are unequally matched, with an X and Y pairing up to create a male. (amherst.edu)
- Note: The DNA from a single human cell has a length of ~1.8 m (but at a width of ~2.4 nanometers). (wikipedia.org)
- Not least, some of the drawbacks of the new technologies for studying modern DNA are advantages when it comes to ancient DNA. (nature.com)
- The objective of this study is to determine whether the amount or type of fungal DNA present in the nose and home environment can be correlated with the outcomes of the following quality of life (QOL) instruments: Medical Outcomes Study 36-item Short Form (SF-36) and the Sino-Nasal Outcomes Study - 20 Questions (SNOT-20). (clinicaltrials.gov)
- The hypothesis is that the quantity and type of fungal DNA present in the nose and home environment are directly correlated with quality of life. (clinicaltrials.gov)
- Stretches of mammoth DNA from a cellular organelle, the mitochondrion, which has its own small genome, have been sequenced previously. (nature.com)
- The objective is to demonstrate person-to-person spread of dermatophytes of the skin and nails among family members, as measured by fungal culture and DNA typing of the dermatophytes isolated. (clinicaltrials.gov)
- All dermatophyte isolates obtained from each family unit are compared using DNA typing and restriction length polymorphism (RFLP) to determine whether family members share the same organism or were infected by an outside source. (clinicaltrials.gov)
- BAL and blood samples will be tested additionally for GM, BDG and with a diagnostic nested Aspergillus PCR assay, a multifungal DNA-Microarray and an azole resistance PCR assay. (knowcancer.com)
- Large DNA viruses of bacteria and eukaryotes have complex life cycles with several distinct phases that involve diverse virus-host interactions. (biomedcentral.com)
- However, Q-PCR allows for exact enumeration of fungal DNA in the tissue. (bio-medicine.org)
- However, in 2005 a new way of sequencing DNA was published 3 . (nature.com)
- Internal Control DNA (High conc. (qiagen.com)