No data available that match "DNA, Bacterial"
bacterial dna - Thaindian Newsbacterial dna. DNA sequencing tracks details of TB outbreak. September 4th, 2012 - 4:01 pm ICT by IANS. Toronto, Sep 4 (IANS) ... Molecular security system protecting cells from harmful DNA discovered. January 11th, 2010 - 1:36 pm ICT by ANI. London, Jan 11 ... the University of Gothenburg in cooperation with Chalmers University of Technology has indicated that the part of bacterial DNA ... natural environment is rising despite tighter controls over our use of antibiotics in medicine and agriculture.Bacterial DNA ...
Electromagnetic signals are produced by aqueous nanostructures derived from bacterial DNA sequences | SpringerLinkA novel property of DNA is described: the capacity of some bacterial DNA sequences to induce electromagnetic waves at high ... Electromagnetic signals are produced by aqueous nanostructures derived from bacterial DNA sequences. ... The genomic DNA of most pathogenic bacteria contains sequences which are able to generate such signals. This opens the way to ... the development of highly sensitive detection system for chronic bacterial infections in human and animal diseases. ...
Exploiting bacterial DNA gyrase as a drug target: current state and perspectives.Abstract DNA gyrase is a type II topoisomerase that can introduce negative supercoils into DNA at the expense of ATP hydrolysis ... Exploiting bacterial DNA gyrase as a drug target: current state and perspectives.. ... Abstract DNA gyrase is a type II topoisomerase that can introduce negative supercoils into DNA at the expense of ATP hydrolysis ... The fluoroquinolones are examples of very successful gyrase-targeted drugs, but the rise in bacterial resistance to these ...
Bacterial DNA in 13th-century Troy bones provides snapshot of maternal infection - Langley AdvanceBacterial DNA in 13th-century Troy bones provides snapshot of maternal infection. 13th-century Troy bones a bacterial reservoir ... "In this case, we just got unbelievable amounts of ancient bacterial DNA that were emphatically not tuberculosis." ... who in turn got in touch with Poinar to see what his lab could make of the bacterial DNA within the "ghost" cells captured ... The 30-year-old woman had been pregnant â€" researchers were able to extract the woman's DNA and that of her male fetus â€" and ...
Detection of bacterial DNA in serum and ascitic fluid of asymptomatic outpatients with cirrhosis and non-neutrocytic ascites -...Detection of bacterial DNA in serum and ascitic fluid of asymptomatic outpatients with cirrhosis and non-neutrocytic ascites. ... Detection of bacterial DNA in serum and ascitic fluid of asymptomatic outpatients with cirrhosis and non-neutrocytic ascites. ...
Direct detection of single-nucleotide polymorphisms in bacterial DNA by SNPtrap - DTU OrbitDirect detection of single-nucleotide polymorphisms in bacterial DNA by SNPtrap. Publication: Research - peer-review › Journal ... Cost-effective optimization of real-time PCR based detection of Campylobacter and Salmonella with inhibitor tolerant DNA ...
Biosensors | Free Full-Text | Microfluidic-Based Amplification-Free Bacterial DNA Detection by Dielectrophoretic Concentration...The assay described in this work incorporates on-chip dielectrophoretic capture and concentration of bacterial cells, thermal ... has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to ...
Improved bacterial strains for therapeutic DNA production | SBIR.govPlasmid DNA produced by bacterial fermentations requires extensive processing to remove endotoxin and purification and re- ... Bacterial strains capable of producing high quality plasmid DNA with minimal processing requirements will significantly improve ... The advent of DNA based vaccines, gene therapy approaches and plasmid based RNA interference (RNAi) has opened the way for DNA ... These new Clean Genome E. coli strains will be of great medical benefit in providing large quantities of safer DNA at low cost ...
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. | Sigma-AldrichA programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.. [Martin Jinek, Krzysztof Chylinski, Ines ... Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to ... breaks in target DNA. At sites complementary to the crRNA-guide sequence, the Cas9 HNH nuclease domain cleaves the ...
Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with...Bacterial Typing Techniques, Base Sequence, DNA, Bacterial/genetics, DNA, Bacterial/isolation & purification, ... Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with ... Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with ... The polymerase chain reaction was used to obtain randomly-amplified polymorphic DNA (RAPD) profiles from Listeria spp. and ...
Plant defenses prompt bacterial countermeasure in the form of island DNA excisionPlant defenses prompt bacterial countermeasure in the form of island DNA excision. 20.12.2005 ... If some bacterial proteins give away the presence of the pathogen to the plant, why are they and their surrounding genomic ... But this genomic island also encodes enzymes that, when switched on, snip the DNA on either side of the island, resulting in ... The reason the strategy can be successful is that the plant has evolved to recognize the presence of only certain bacterial ...
PLOS Pathogens: Effective, Broad Spectrum Control of Virulent Bacterial Infections Using Cationic DNA Liposome Complexes...This compound is comprised of cationic liposome DNA complexes (CLDC) and crude membrane preparations (MPF) obtained from ... Thus, CLDC+MPF represents a novel antimicrobial for treatment of lethal, acute, bacterial infections. ... Author Summary Conventional treatment of bacterial infections typically includes administration of antibiotics. However, many ...
How does all that DNA fit into the cell? Studies of bacterial chromosome organisation proteins (LE J17DTP) - University of East...How does all that DNA fit into the cell? Studies of bacterial chromosome organisation proteins (LE_J17DTP). University of East ...
AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis. - Oxford NeuroscienceOxidative stress is also used by phagocytes to attack bacterial pathogens in defence of the host. We have identified and ... The presence of multiple versions of DNA repair enzymes in a single organism is usually thought to reflect redundancy in ... We demonstrate here that these two AP endonuclease paralogues have distinct activities in DNA repair: one is a typical ... Oxidative stress is a principal cause of DNA damage, and mechanisms to repair this damage are among the most highly conserved ...
A microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from low copy/single bacterial...We developed a novel microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA ... Purification of nucleic acids from low quantity of bacterial cells in minute volume is im- portant in many clinical and ... A microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from low copy/single bacterial ... We developed a novel microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from bacterial ...
Marine Drugs | Free Full-Text | Antioxidant and Antimicrobial Potential of the Bifurcaria bifurcata Epiphytic Bacteria | HTMLBacterial DNA Extraction and Identification. DNA was extracted from previously stored bacterial pellets with the GeneJET™ ... Flexi DNA Polymerase 5U/µL and 1 µL of DNA template. Thermal cycling started with an initial denaturation step of 94 °C for 3 ... Ducklow, H. Bacterial Production and Biomass in the Ocean. In Microbial Ecology of the Oceans, 1st ed.; Kirchman, D.L., Ed.; ... From an ecological point of view, the inhibition of other marine bacterial species competing for the same niche will give a ...
Peritoneal tuberculosis: diagnostic options. - PubMed - NCBIDNA, Bacterial. LinkOut - more resources. Full Text Sources. *Hindawi Publishing Corporation. *Europe PubMed Central ... Tuberculosis was confirmed by DNA extraction from the frozen section specimen with subsequent analysis using polymerase chain ... Newer tests such as DNA or RNA amplification allow for early diagnosis but have limitations. ...
High prevalence of Borrelia burgdorferi s.l. in the European red squirrel Sciurus vulgaris in France. - PubMed - NCBIDNA of B. burgdorferi genospecies were detected and identified from PCR products in ear biopsies using reverse line blot ... DNA, Bacterial/chemistry. *DNA, Bacterial/genetics. *Disease Reservoirs. *Female. *France/epidemiology. *Geography ...
Bartonella DNA in the blood and lymph nodes of Golden Retrievers with lymphoma and in healthy controls. - PubMed - NCBIDNA, Bacterial/analysis*. *DNA, Bacterial/blood. *Disease Vectors. *Dog Diseases/blood. *Dog Diseases/microbiology* ... Bartonella DNA in the blood and lymph nodes of Golden Retrievers with lymphoma and in healthy controls.. Duncan AW1, Marr HS, ... Bartonella DNA can be detected in blood and lymph nodes; importantly, in this report, Bartonella was detected in the same ... Using PCR analyses and DNA sequencing, single and coinfections with Bartonella henselae, Bartonella elizabethae, Bartonella ...
Genotype to phenotype: identification of diagnostic vibrio phenotypes using whole genome sequences. - PubMed - NCBIBacterial Typing Techniques/methods*. *Classification/methods. *DNA, Bacterial/genetics. *Genome, Bacterial*. *Genotype ...
Diagnostic and Interventional Therapy in Acute Pancreatitis - Full Text View - ClinicalTrials.govBiospecimen Retention: Samples With DNA. Bacterial DNA Estimated Enrollment:. 50. Study Start Date:. May 2008. ...
Murine Lung Microbiome Webinar Recap | Taconic BiosciencesAs the bacterial DNA yield of the lungs is very low relative to the gut, it is particularly challenging to perform DNA ... Q: Can you separate the host DNA from the bacterial DNA?. KKB: We spun the BAL fluids to separate mouse cells from the ... The murine lung microbiome in relation to the intestinal and vaginal bacterial communities. BMC Microbiol. 2013, 13, 303 DOI: ...
Perio 6 Treatment of Periodontal Disease; Antibiotics and antibiotic delivery systems-DoctorSpiller.com - DoctorSpiller.comLaboratory testing of saliva for bacterial DNA and Interlukin-1. The bacterial flora in plaque varies from patient to patient. ... DNA testing can ferret out bacterial species that are difficult or impossible to culture by ordinary means. This has opened up ... The suppression of bacterial activity in the pocket for almost a month gives the body plenty of time to heal. Arestin has ... It has been found that analysis of a patient's saliva can reveal the DNA of the specific organisms causing the immune reaction ...
Cloning and sequencing of the ilvBNC gene cluster from Mycobacterium avium.DNA, Bacterial. Ketol-Acid Reductoisomerase. Molecular Sequence Data. Multigene Family*. Mycobacterium avium / enzymology, ... 0/DNA, Bacterial; EC 1.1.-/Alcohol Oxidoreductases; EC 184.108.40.206/Ketol-Acid Reductoisomerase; EC 220.127.116.11/Acetolactate Synthase ... The deduced amino acid sequences revealed significant homology to the AHS and IR proteins from other bacterial species.. ...
History CommonsAlso, Escherichia Coli (E.Coli); genetic materials; human and bacterial DNA. [CounterPunch, 8/20/2002]. VX nerve gas. [Sunday ...
No data available that match "DNA, Bacterial"
(1/38768) Detection of Chlamydia pneumoniae but not cytomegalovirus in occluded saphenous vein coronary artery bypass grafts.
BACKGROUND: A causal relation between atherosclerosis and chronic infection with Chlamydia pneumoniae and/or cytomegalovirus (CMV) has been suggested. Whether the unresolved problem of venous coronary artery bypass graft occlusion is related to infection with C pneumoniae and/or CMV has not been addressed. METHODS AND RESUTLS: Thirty-eight occluded coronary artery vein grafts and 20 native saphenous veins were examined. Detection of C pneumoniae DNA was performed by use of nested polymerase chain reaction (PCR). Homogenisates from the specimen were cultured for identification of viable C pneumoniae. Both conventional PCR and quantitative PCR for detection of CMV DNA were applied. Differential pathological changes (degree of inflammation, smooth muscle cell proliferation [MIB-1]) were determined and correlated to the detection of both microorganisms. C pneumoniae DNA could be detected in 25% of occluded vein grafts. Viable C pneumoniae was recovered from 16% of occluded vein grafts. Except for 1 native saphenous vein, all control vessels were negative for both C pneumoniae detection and culture. All pathological and control specimens were negative for CMV DNA detection. Pathological changes did not correlate with C pneumoniae detection. CONCLUSIONS: Occluded aorto-coronary venous grafts harbor C pneumoniae but not CMV. The detection of C pneumoniae in occluded vein grafts warrants further investigation. (+info)
(2/38768) Acinetobacter bacteremia in Hong Kong: prospective study and review.
The epidemiological characteristics of 18 patients with acinetobacter bacteremia were analyzed. Patients (mean age, 55.5 years) developed bacteremia after an average of 14.1 days of hospitalization. Fifteen of 16 patients survived bacteremia caused by Acinetobacter baumannii. Cultures of blood from the remaining two patients yielded Acinetobacter lwoffii. Most patients (78%) resided in the general ward, while four patients (22%) were under intensive care. Genotyping by arbitrarily primed polymerase chain reaction analysis and the temporal sequence of isolation were more useful than phenotyping by antimicrobial susceptibility in the determination of the source of bacteremia, and the intravascular catheter was the leading infection source (39% of cases). The possibility of an association of glucose with the pathogenesis of acinetobacter infection was raised. (+info)
(3/38768) Legionnaires' disease on a cruise ship linked to the water supply system: clinical and public health implications.
The occurrence of legionnaires' disease has been described previously in passengers of cruise ships, but determination of the source has been rare. A 67-year-old, male cigarette smoker with heart disease contracted legionnaires' disease during a cruise in September 1995 and died 9 days after disembarking. Legionella pneumophila serogroup 1 was isolated from the patient's sputum and the ship's water supply. Samples from the air-conditioning system were negative. L. pneumophila serogroup 1 isolates from the water supply matched the patient's isolate, by both monoclonal antibody subtyping and genomic fingerprinting. None of 116 crew members had significant antibody titers to L. pneumophila serogroup 1. One clinically suspected case of legionnaires' disease and one confirmed case were subsequently diagnosed among passengers cruising on the same ship in November 1995 and October 1996, respectively. This is the first documented evidence of the involvement of a water supply system in the transmission of legionella infection on ships. These cases were identified because of the presence of a unique international system of surveillance and collaboration between public health authorities. (+info)
(4/38768) Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov.
A polyphasic taxonomic study was undertaken to clarify relationships within and between representative thermophilic alkalitolerant streptomycetes isolated from soil and appropriate marker strains. The resultant data, notably those from DNA-DNA relatedness studies, support the taxonomic integrity of the validly described species Streptomyces thermodiastaticus, Streptomyces thermoviolaceus and Streptomyces thermovulgaris. However, the genotypic and phenotypic data clearly show that Streptomyces thermonitrificans Desai and Dhala 1967 and S. thermovulgaris (Henssen 1957) Goodfellow et al. 1987 represent a single species. On the basis of priority, S. thermonitrificans is a later subjective synonym of S. thermovulgaris. Similarly, 10 out of the 11 representative thermophilic alkalitolerant isolates had a combination of properties consistent with their classification as S. thermovulgaris. The remaining thermophilic alkalitolerant isolate, Streptomyces strain TA56, merited species status. The name Streptomyces thermoalcalitolerans sp. nov. is proposed for this strain. A neutrophilic thermophilic isolate, Streptomyces strain NAR85, was identified as S. thermodiastaticus. (+info)
(5/38768) Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively.
Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively. (+info)
(6/38768) Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.
The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as 'biotype A' of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov. corresponds to [P.] haemolytica biogroups 3A-3H and the beta-glucosidase and meso-inositol-positive strains of [P.] haemolytica biogroup 9. All typable strains within M. glucosida belong to serotype 11. Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.] haemolytica biogroup 8D. Finally, Mannheimia varigena sp. nov. includes [P.] haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M. haemolytica), ATCC 49244T (M. granulomatis), CCUG 38457T = P925T (M. glucosida), CCUG 38470T = HPA92T (M. ruminalis) and CCUG 38462T = 177T (M. varigena). (+info)
(7/38768) Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization.
The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA gyrase B subunit. The phylogenetic tree showed linkages between genomic species 1 (Acinetobacter calcoaceticus), 2 (Acinetobacter baumannii), 3 and TU13; genomic species 6, BJ15, BJ16 and BJ17; genomic species 5, BJ13 (synonym of TU14) and BJ14; genomic species 7 (Acinetobacter johnsonii), 10 and 11; and genomic species 8 and 9. The phylogenetic grouping of Acinetobacter strains based on gyrB genes was almost congruent with that based on DNA-DNA hybridization studies. Consequently, gyrB sequence comparison can be used to resolve the taxonomic positions of bacterial strains at the level of genomic species. However, minor discrepancies existed in the grouping of strains of genomic species 8, 9 and BJ17. The phylogenetic tree for these strains was reconstructed from the sequence of rpoD, the structural gene for the RNA polymerase sigma 70 factor. The latter tree was 100% congruent with the grouping based on DNA-DNA hybridization. The reliability of DNA-DNA hybridization may be superior to that of sequence comparison of a single protein-encoding gene in resolving closely related organisms since the former method measures the homologies between the nucleotide sequences of total genomic DNAs. Three strains that have not been characterized previously by DNA-DNA hybridization seem to belong to two new genomic species, one including strain ATCC 33308 and the other including strains ATCC 31012 and MBIC 1332. (+info)
(8/38768) Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake.
Eight Gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic Ekho Lake (Vestfold Hills, East Antarctica). The cells contained storage granules and daughter cells could be motile. Bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. The strains tolerated a wide range of temperature, pH, concentrations of artificial seawater and NaCl, but had an absolute requirement for sodium ions. Glutamate was metabolized with and without an additional source of combined nitrogen. The dominant fatty acid was C18:1; other characteristic fatty acids were C18:2, C12:0 2-OH, C12:1 3-OH, C16:1, C16:0 and C18:0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C base composition was 62-64 mol%. 16S rRNA gene sequence comparisons showed that the isolates were phylogenetically close to the genera Antarctobacter, 'Marinosulfonomonas', Octadecabacter, Sagittula, Sulfitobacter and Roseobacter. Morphological, physiological and genotypic differences to these previously described and distinct genera support the description of a new genus and a new species, Roseovarius tolerans gen. nov., sp. nov. The type strain is EL-172T (= DSM 11457T). (+info)
- In the new work, researchers have essentially caught one step of this arms race in action, and they have shed light on the molecular mechanisms employed by a bacterial pathogen to survive in the face of its host plant s defenses. (innovations-report.com)
- In their study, the authors identify within the halo-bright pathogen genome a special island of DNA that encodes one such offending protein. (innovations-report.com)
- If some bacterial proteins give away the presence of the pathogen to the plant, why are they and their surrounding genomic islands maintained by the bacteria at all? (innovations-report.com)
- These findings offer a molecular explanation for how exposure to plant resistance mechanisms can directly drive the evolution of new virulent forms of a bacterial pathogen. (innovations-report.com)
- Our microfluidic platform offered a simple and effective solution for nucleic acid preparation, which can be integrated for automated bacterial pathogen detection and high throughput transcriptional profiling. (biomedsearch.com)
- Potential contaminants include host proteins, transposable elements that can jump from the host into the product plasmid DNA and highly toxic lipopolysaccharide moieties from the outer membrane of the bacteria collectively known as endotoxin. (sbir.gov)
- The reason the strategy can be successful is that the plant has evolved to recognize the presence of only certain bacterial proteins as warnings of an infection. (innovations-report.com)
- This opens the way to the development of highly sensitive detection system for chronic bacterial infections in human and animal diseases. (springer.com)
- A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. (sigmaaldrich.com)
- AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis. (ox.ac.uk)
- We demonstrate here that these two AP endonuclease paralogues have distinct activities in DNA repair: one is a typical Neisserial AP endonuclease (NApe), whereas the other is a specialised 3'-phosphodiesterase Neisserial exonuclease (NExo). (ox.ac.uk)
- Plasmid DNA produced by bacterial fermentations requires extensive processing to remove endotoxin and purification and re-purification is a significant source of the high cost of clinical grade DNA. (sbir.gov)
- A microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from low copy/single bacterial cells in minute sample volume followed by direct on-chip quantitative PCR assay. (biomedsearch.com)
- Purification of nucleic acids from low quantity of bacterial cells in minute volume is im- portant in many clinical and biological applications. (biomedsearch.com)
- We developed a novel microfluidic liquid phase nucleic acid purification chip to selectively isolate DNA or RNA from bacterial cell in the range of 5000 down to a single cell in the sample volume of 1 µl or 125 nl, which can be directly put through on-chip quantitative PCR assay. (biomedsearch.com)
- A novel property of DNA is described: the capacity of some bacterial DNA sequences to induce electromagnetic waves at high aqueous dilutions. (springer.com)
- The aqueous phase bacterial lysate was isolated in an array of micro-wells, after which an immiscible organic (phenol-chloroform) phase was introduced in a headspace channel connecting the micro-well array. (biomedsearch.com)
- Random amplification of polymorphic bacterial DNA: evaluation of 11 oligonucleotides and application to food contaminated with Listeria monocytogenes. (unil.ch)
- Newer tests such as DNA or RNA amplification allow for early diagnosis but have limitations. (nih.gov)
- In this case, we just got unbelievable amounts of ancient bacterial DNA that were emphatically not tuberculosis. (langleyadvance.com)
- Tuberculosis was confirmed by DNA extraction from the frozen section specimen with subsequent analysis using polymerase chain reaction. (nih.gov)
- E. coli K12 has been used for decades to produce plasmid DNA for molecular biology research and this methodology has in general simply been extended to manufacturing practice. (sbir.gov)
- The genomic DNA of most pathogenic bacteria contains sequences which are able to generate such signals. (springer.com)
- Excising this so-called "genomic island" eliminates production of the bacterial protein detected by the plant and allows a more stealthy - and successful - invasion. (innovations-report.com)
- But this genomic island also encodes enzymes that, when switched on, snip the DNA on either side of the island, resulting in the excision of the entire island from the genome. (innovations-report.com)
- An international team of scientists, including experts at McMaster University in Hamilton, have sequenced the genomes of two types of bacteria, whose DNA was preserved in calcified placental abscesses found in the skeleton of a woman who died in Byzantine Troy about 800 years ago. (langleyadvance.com)
- The 30-year-old woman had been pregnant â€" researchers were able to extract the woman's DNA and that of her male fetus â€" and had likely died of a urogenital infection caused by one or both of the bacteria, Gardnerella vaginalis and Staphylococcus saprophyticus, which cause genital and urinary tract infections in women to this day. (langleyadvance.com)
- Co-principal investigator Hendrik Poinar, an evolutionary biologist at McMaster who specializes in ancient DNA, said analysis of genetic material preserved in the remains provides a snapshot of a massive maternal infection after the staph bacteria likely jumped from a cow to the woman. (langleyadvance.com)
- Oxidative stress is a principal cause of DNA damage, and mechanisms to repair this damage are among the most highly conserved of biological processes. (ox.ac.uk)
- The presence of multiple versions of DNA repair enzymes in a single organism is usually thought to reflect redundancy in activities that are essential for cellular viability. (ox.ac.uk)
- Abstract DNA gyrase is a type II topoisomerase that can introduce negative supercoils into DNA at the expense of ATP hydrolysis. (omicsonline.org)
- Abstract DESCRIPTION (provided by applicant): Principal Investigator/Program Director (Last, First, Middle): Blattner, Frederick R. Abstract: The goal of this proposal is to develop methods and strains for manufacturing plasmid DNA of extraordinary purity in very large quantity for therapeutic use. (sbir.gov)
- So I said yes, of course," recalled Pepperell, who in turn got in touch with Poinar to see what his lab could make of the bacterial DNA within the "ghost" cells captured inside the mineralized nodules. (langleyadvance.com)
- Using this nucleic acid purifi- cation device set in a two-dimensional array format of 900 micro-wells, it was demonstrated for the first time that high throughput extraction of RNA couple with direct on-chip PCR analysis from single bacterial cells could be achieved. (biomedsearch.com)
- Bacterial strains capable of producing high quality plasmid DNA with minimal processing requirements will significantly improve the cost, yield and safety of clinical grade plasmid DNA. (sbir.gov)
- How does all that DNA fit into the cell? (jobs.ac.uk)
- Our study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing. (sigmaaldrich.com)
- Oxidative stress is also used by phagocytes to attack bacterial pathogens in defence of the host. (ox.ac.uk)
- These new Clean Genome E. coli strains will be of great medical benefit in providing large quantities of safer DNA at low cost for therapeutic use. (sbir.gov)
- The fluoroquinolones are examples of very successful gyrase-targeted drugs, but the rise in bacterial resistance to these agents means that we not only need to seek new compounds, but also new modes of inhibition of this enzyme. (omicsonline.org)
- We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in target DNA. (sigmaaldrich.com)