No data available that match "DNA, Bacterial"



*  Nanopore detection of bacterial DNA base modifications

The common bacterial base modification N6-methyladenine (m6A) is involved in many pathways related to an organism's ability to ... Nanopore detection of bacterial DNA base modifications 13th April 2017 - BioRxiv. The common bacterial base modification N6- ... the ability to reliably characterize m6A presents an opportunity to further examine the role of methylation in bacterial ...

*  AllPrep Bacterial DNA/RNA/Protein Kit - QIAGEN Online Shop

For the extraction of protein and nucleic acids from bacterial cell cultures ... Easy-to-use, the AllPrep Bacterial DNA/RNA/Protein Kit isolates total nucleic acids and cellular proteins from bacterial ... Home − Shop − Sample Technologies − RNA − Total RNA − Allprep Bacterial DNA/RNA/Protein Kit ... The Allprep Bacterial DNA/RNA/Protein Kit is intended for molecular biology applications. This product is not intended for the ...

*  DNA/RNA tunnel of bacterial DNA dependent RNA polymerase (IPR021975) | InterPro | EMBL-EBI

DNA/RNA tunnel of bacterial DNA dependent RNA polymerase (IPR021975). Short name: RNApol_Rpb2_rif ... This domain is part of the beta subunit of bacterial DNA dependent RNA polymerase. This domain is the binding site for the ... antibacterial drug rifampin (and its analogues) which blocks the DNA/RNA tunnel and prevents initiation of transcription. ...

*  Bacterial DNA binding protein - Wikipedia

... bacterial DNA binding proteins were thought to help stabilize bacterial DNA. Currently, many more functions of bacteria DNA ... Research suggests that bacterial DNA binding protein has an important role during DNA replication; the protein is involved in ... The functions of bacterial DNA-binding proteins are not limited to DNA replication. Researchers have been investigating other ... These proteins participate in all DNA-dependent functions; in these processes, bacterial DNA binding proteins have an ...

*  DNA & RNA Purification Bacterial Genomic Miniprep Kit | China-Mainland | Sigma-Aldrich

The GenElute Bacterial Genomic Kit provides a simple and convenient technique to isolate high quality DNA from both Gram(-) and ... Figure 3. Purified genomic DNA was isolated from various bacterial species using the GenElute Bacterial Genomic DNA kit. A 1 µg ... Typical DNA Yields with the GenElute Bacterial Genomic DNA Kit.. Source. Type of Media. Amount of Overnight Culture. OD600 per ... The GenElute Bacterial Genomic DNA Kit contains all of the reagents needed to purify genomic DNA from Gram negative bacteria ( ...

*  A Magical Journey: Scientists reveal hidden structures in bacterial DNA

DNA contains the instructions for life, encoded within genes. Within all cells, DNA is organised into very long lengths known ... and can be achieved with little more than a handful of DNA binding proteins and the structural properties of the DNA itself.. ... This is a similar arrangement to that seen in some other larger bacterial species.. The scientists also used the Hi-C technique ... Using a technique called Hi-C, which reveals the interactions between different pieces of DNA, the researchers created a three- ...

*  Practical Fragments: Fragments vs bacterial DNA ligase: triumph of structure-based design

The researchers were interested in bacterial DNA ligase (LigA), which is essential for DNA replication, is highly conserved ... Fragment approaches have been used successfully against several anti-bacterial targets (see for example here, here, and here). ...

*  Bacterial Infection Diagnosis Using Blood DNA - Full Text View - ClinicalTrials.gov

Biospecimen Retention: Samples With DNA. Sample with bacterial DNA (and no human DNA will be studied) ... Bacterial Infection Diagnosis Using Blood DNA. The safety and scientific validity of this study is the responsibility of the ... The goal of the current study is to evaluate the diagnostic value of plasma detection of bacterial DNA in ICU patients with a ... Bacterial DNA Detection as a Diagnostic Tool of Infection in Critical Ill Patients With SIRS. ...

*  British Library EThOS: Nanoscale patterning of complex DNA structures with the bacterial protein Recombinase A

The bacterial protein Recombinase A (RecA) may be able to provide this function. This thesis will discuss the patterning of DNA ... Nanoscale patterning of complex DNA structures with the bacterial protein Recombinase A ... To test the potential of RecA to pattern more complex DNA, novel strategies for creating DNA topologies have been explored. ... The use of DNA as a structural material has been intensively developed since its inception in the early 1980s. The potential of ...

*  Identification of a Renibacterium salmoninarum DNA fragment associated with bacterial internalization into CHSE-cultured cells ...

...

*  How to Show the Real Microbial Biodiversity? A Comparison of Seven DNA Extraction Methods for Bacterial Population Analyses in...

How to Show the Real Microbial Biodiversity? A Comparison of Seven DNA Extraction Methods for Bacterial Population Analyses in ... A DNA extraction that comprises the DNA of all available taxa in an ecosystem is an essential step in population analysis, ... bacteria, biodiversity, DNA-extraction, ecology National Category Microbiology Research subject. Biology with specialization in ... The amount and the quality of nucleic acid extracts were determined and compared to the amplifiable amount of DNA. The 16S rRNA ...

*  Effective, Broad Spectrum Control of Virulent Bacterial Infections Using Cationic DNA Liposome Complexes Combined with...

This compound is comprised of cationic liposome DNA complexes (CLDC) and crude membrane preparations (MPF) obtained from ... Thus, CLDC+MPF represents a novel antimicrobial for treatment of lethal, acute, bacterial infections. ... Author Summary Conventional treatment of bacterial infections typically includes administration of antibiotics. However, many ... Bacterial pathogens Is the Subject Area "Bacterial pathogens" applicable to this article? Yes. No. ...

*  How does all that DNA fit into the cell? Studies of bacterial chromosome organisation proteins (LE J17DTP) - University of East...

How does all that DNA fit into the cell? Studies of bacterial chromosome organisation proteins (LE_J17DTP). University of East ...

*  "From Host Recognition to T-Dna Integration: the Function of Bacterial " by Tzvi Tzfira and Vitaly Citovsky

Tzfira, Tzvi and Citovsky, Vitaly, "From Host Recognition to T-Dna Integration: the Function of Bacterial and Plant Genes in ... From Host Recognition to T-Dna Integration: the Function of Bacterial and Plant Genes in the Agrobacterium-Plant Cell ...

*  Modification of Bacterial Artificial Chromosomes (BACs) and Preparation of Intact BAC DNA for Generation of Transgenic Mice -...

If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...

*  Recent Articles | Bacteria And Culture | The Scientist Magazine®| Page 40

Researchers use DNA from ancient tooth tartar to chart changes in the bacterial communities that have lived in human mouths for ...

*  Find other content tagged with 'bacteria'

Scientists Replay Movie Encoded in Bacterial DNA Reza posted a topic in Science/Tech/Economics ... Afterwards, they were able to reconstruct the movie with 90 percent accuracy by sequencing the bacterial DNA. Although this ... "These could be used to model disease - or even in therapies." For starters, the researchers had to show that DNA can be used to ... CRISPR is a group of proteins and DNA that act as an immune system in some bacteria, vaccinating them with genetic memories of ...

*  IJERPH | Free Full-Text | Effect of Ampicillin, Streptomycin, Penicillin and Tetracycline on Metal Resistant and Non-Resistant...

Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain ... Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were ... which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to ... There is an arising and concerning issue in the field of bacterial resistance, ...

*  Tyrrell Conway

DNA Microarray / Bacterial Functional Genomics Home Page. Selected Publications:. Traxler, M. F., S. M. Summers, H. T. Nguyen, ... We are comparing commensal (innocuous) E. coli strains with the pathogen E. coli O157:H7, for which we have fabricated DNA ... In: 4th Edition Virulence Mechanisms of Bacterial Pathogens. K.A. Brogden, F.C. Minion, N. Cornick, T.B. Stanton, Q. Zhang, L.K ... Role of the mucus layer in bacterial colonization of the intestine. In: Colonization of Mucosal Surfaces, Nataro, J. P., H. L. ...

*  Antibiotic resistance of Escherichia Coli isolates from environmental and waste water samples in Mauritius. - Free Online...

Extraction of bacterial DNA: [E.sub.W2] [F.sub.GF1], [H.sub.WO], [I.sub.C] and [F.sub.C] isolates were grown overnight in Luria ... DNA polymerase, 2[chi] [micro]l Primer 1 and 2[chi] Lil Primer 2. The final volume was 30 [micro]l. [chi square]: number of ... DNA was extracted from a few isolates and tested in PCR for the presence of beta-lactamase genes. PCR amplification results ... A ladder was also loaded as a DNA size marker and was electrophoresed in TBE buffer with a voltage of 90V. Ethidium bromide ( ...

*  Transcription of the extended hyp-operon in Nostoc sp. strain PCC 7120.

Bacterial Proteins / genetics. Base Pairing. Chromosome Mapping. Conserved Sequence. DNA, Bacterial / analysis, genetics. ... Obtained DNA fragments were isolated from the agarose gels using the GFX PCR DNA and Gel Band Purification Kit (GE Healthcare ... Genomic DNA was isolated from Nostoc sp. strain PCC 7120 cultures as described [42]. For total RNA isolation Nostoc sp. strain ... PCR, DNA sequencing and sequence analysis PCR amplifications were carried out using Eppendorf Mastermix (2.5?) (Eppendorf) in a ...

*  Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 Carries N,N-Dimethylformamide Degradation-Related Genes Whose Expression Is...

Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. Nature 417:971-974. [PubMed] ... Introduction of plasmid DNA into bacterial cells. Transformation of E. coli TG1 was performed according to the method of ... DNA manipulations. Plasmid DNA was isolated according to the procedure of Birnboim and Doly (4) and, when required, further ... DNA sequencing. The nucleotide sequence of plasmid pAMI2 was determined in the DNA Sequencing and Oligonucleotide Synthesis ...

*  Patent US8222225 - Method of treating pneumoconiosis with oligodeoxynucleotides - Google Patents

CpG motifs are found in bacterial DNA. The innate immune response elicited by CpG DNA reduces host susceptibility to infectious ... Schwartz et al., CpG Motifs in Bacterial DNA Cause Inflammation in the Lower Respiratory Tract, J. Clin. Invest. 100:68-73 ( ... Klinman et al., "CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and ... Klinman et al., "CpG motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and ...

*  Brevet WO1999051259A2 - Methods and products for stimulating the immune system using ... - Google Brevets

... and vertebrate DNA, which does not, is that bacterial DNA contains a higher frequency of unmethylated CpG dinucleotides than ... Recently, bacterial DNA itself has been reported to be one such molecule (e.g., Krieg, A.M., et al, 1995, Nature 374:546-9). ... Receptor protein specifically recognizing bacterial dna. WO2002036141A2 *. 30 oct. 2001. 10 mai 2002. Immunex Corporation. ... "CpG DNA" or DNA containing a 5' cytosine followed by 3' guanosine and linked by a phosphate bond) and activate the immune ...

*  Biomatrica | China-Mainland | Sigma-Aldrich

Preserve and Store Bacterial DNA. CloneStable® enables the long-term storage of unpurified bacterial plasmid and genomic DNA. ... The liquid storage reagent rapidly permeates cell membranes to stabilize and protect genomic DNA. *DNAgard® Tissue Trial Kit ... DNAgard® offers immediate stabilization of DNA from intact cells and tissues with the convenience of room temperature shipping ...

No data available that match "DNA, Bacterial"



(1/38768) Detection of Chlamydia pneumoniae but not cytomegalovirus in occluded saphenous vein coronary artery bypass grafts.

BACKGROUND: A causal relation between atherosclerosis and chronic infection with Chlamydia pneumoniae and/or cytomegalovirus (CMV) has been suggested. Whether the unresolved problem of venous coronary artery bypass graft occlusion is related to infection with C pneumoniae and/or CMV has not been addressed. METHODS AND RESUTLS: Thirty-eight occluded coronary artery vein grafts and 20 native saphenous veins were examined. Detection of C pneumoniae DNA was performed by use of nested polymerase chain reaction (PCR). Homogenisates from the specimen were cultured for identification of viable C pneumoniae. Both conventional PCR and quantitative PCR for detection of CMV DNA were applied. Differential pathological changes (degree of inflammation, smooth muscle cell proliferation [MIB-1]) were determined and correlated to the detection of both microorganisms. C pneumoniae DNA could be detected in 25% of occluded vein grafts. Viable C pneumoniae was recovered from 16% of occluded vein grafts. Except for 1 native saphenous vein, all control vessels were negative for both C pneumoniae detection and culture. All pathological and control specimens were negative for CMV DNA detection. Pathological changes did not correlate with C pneumoniae detection. CONCLUSIONS: Occluded aorto-coronary venous grafts harbor C pneumoniae but not CMV. The detection of C pneumoniae in occluded vein grafts warrants further investigation.  (+info)

(2/38768) Acinetobacter bacteremia in Hong Kong: prospective study and review.

The epidemiological characteristics of 18 patients with acinetobacter bacteremia were analyzed. Patients (mean age, 55.5 years) developed bacteremia after an average of 14.1 days of hospitalization. Fifteen of 16 patients survived bacteremia caused by Acinetobacter baumannii. Cultures of blood from the remaining two patients yielded Acinetobacter lwoffii. Most patients (78%) resided in the general ward, while four patients (22%) were under intensive care. Genotyping by arbitrarily primed polymerase chain reaction analysis and the temporal sequence of isolation were more useful than phenotyping by antimicrobial susceptibility in the determination of the source of bacteremia, and the intravascular catheter was the leading infection source (39% of cases). The possibility of an association of glucose with the pathogenesis of acinetobacter infection was raised.  (+info)

(3/38768) Legionnaires' disease on a cruise ship linked to the water supply system: clinical and public health implications.

The occurrence of legionnaires' disease has been described previously in passengers of cruise ships, but determination of the source has been rare. A 67-year-old, male cigarette smoker with heart disease contracted legionnaires' disease during a cruise in September 1995 and died 9 days after disembarking. Legionella pneumophila serogroup 1 was isolated from the patient's sputum and the ship's water supply. Samples from the air-conditioning system were negative. L. pneumophila serogroup 1 isolates from the water supply matched the patient's isolate, by both monoclonal antibody subtyping and genomic fingerprinting. None of 116 crew members had significant antibody titers to L. pneumophila serogroup 1. One clinically suspected case of legionnaires' disease and one confirmed case were subsequently diagnosed among passengers cruising on the same ship in November 1995 and October 1996, respectively. This is the first documented evidence of the involvement of a water supply system in the transmission of legionella infection on ships. These cases were identified because of the presence of a unique international system of surveillance and collaboration between public health authorities.  (+info)

(4/38768) Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov.

A polyphasic taxonomic study was undertaken to clarify relationships within and between representative thermophilic alkalitolerant streptomycetes isolated from soil and appropriate marker strains. The resultant data, notably those from DNA-DNA relatedness studies, support the taxonomic integrity of the validly described species Streptomyces thermodiastaticus, Streptomyces thermoviolaceus and Streptomyces thermovulgaris. However, the genotypic and phenotypic data clearly show that Streptomyces thermonitrificans Desai and Dhala 1967 and S. thermovulgaris (Henssen 1957) Goodfellow et al. 1987 represent a single species. On the basis of priority, S. thermonitrificans is a later subjective synonym of S. thermovulgaris. Similarly, 10 out of the 11 representative thermophilic alkalitolerant isolates had a combination of properties consistent with their classification as S. thermovulgaris. The remaining thermophilic alkalitolerant isolate, Streptomyces strain TA56, merited species status. The name Streptomyces thermoalcalitolerans sp. nov. is proposed for this strain. A neutrophilic thermophilic isolate, Streptomyces strain NAR85, was identified as S. thermodiastaticus.  (+info)

(5/38768) Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively.

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.  (+info)

(6/38768) Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.

The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as 'biotype A' of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov. corresponds to [P.] haemolytica biogroups 3A-3H and the beta-glucosidase and meso-inositol-positive strains of [P.] haemolytica biogroup 9. All typable strains within M. glucosida belong to serotype 11. Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.] haemolytica biogroup 8D. Finally, Mannheimia varigena sp. nov. includes [P.] haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M. haemolytica), ATCC 49244T (M. granulomatis), CCUG 38457T = P925T (M. glucosida), CCUG 38470T = HPA92T (M. ruminalis) and CCUG 38462T = 177T (M. varigena).  (+info)

(7/38768) Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization.

The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA gyrase B subunit. The phylogenetic tree showed linkages between genomic species 1 (Acinetobacter calcoaceticus), 2 (Acinetobacter baumannii), 3 and TU13; genomic species 6, BJ15, BJ16 and BJ17; genomic species 5, BJ13 (synonym of TU14) and BJ14; genomic species 7 (Acinetobacter johnsonii), 10 and 11; and genomic species 8 and 9. The phylogenetic grouping of Acinetobacter strains based on gyrB genes was almost congruent with that based on DNA-DNA hybridization studies. Consequently, gyrB sequence comparison can be used to resolve the taxonomic positions of bacterial strains at the level of genomic species. However, minor discrepancies existed in the grouping of strains of genomic species 8, 9 and BJ17. The phylogenetic tree for these strains was reconstructed from the sequence of rpoD, the structural gene for the RNA polymerase sigma 70 factor. The latter tree was 100% congruent with the grouping based on DNA-DNA hybridization. The reliability of DNA-DNA hybridization may be superior to that of sequence comparison of a single protein-encoding gene in resolving closely related organisms since the former method measures the homologies between the nucleotide sequences of total genomic DNAs. Three strains that have not been characterized previously by DNA-DNA hybridization seem to belong to two new genomic species, one including strain ATCC 33308 and the other including strains ATCC 31012 and MBIC 1332.  (+info)

(8/38768) Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake.

Eight Gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic Ekho Lake (Vestfold Hills, East Antarctica). The cells contained storage granules and daughter cells could be motile. Bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. The strains tolerated a wide range of temperature, pH, concentrations of artificial seawater and NaCl, but had an absolute requirement for sodium ions. Glutamate was metabolized with and without an additional source of combined nitrogen. The dominant fatty acid was C18:1; other characteristic fatty acids were C18:2, C12:0 2-OH, C12:1 3-OH, C16:1, C16:0 and C18:0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C base composition was 62-64 mol%. 16S rRNA gene sequence comparisons showed that the isolates were phylogenetically close to the genera Antarctobacter, 'Marinosulfonomonas', Octadecabacter, Sagittula, Sulfitobacter and Roseobacter. Morphological, physiological and genotypic differences to these previously described and distinct genera support the description of a new genus and a new species, Roseovarius tolerans gen. nov., sp. nov. The type strain is EL-172T (= DSM 11457T).  (+info)



proteins

  • Easy-to-use, the AllPrep Bacterial DNA/RNA/Protein Kit isolates total nucleic acids and cellular proteins from bacterial cultures using spin column technology. (qiagen.com)
  • Using a bead-based lysis and spin-column collection method, nucleic acids and proteins are effectively extracted from Gram (+/-) bacterial species. (qiagen.com)
  • Beginning by lysing cultured bacterial cells using 0.1 mm glass bead tubes, nucleic acids and proteins are made soluble and bound with solution. (qiagen.com)
  • In three steps of binding and flow through, DNA is bound to one silica spin column and the flow-through containing RNA and proteins is processed through a second and third spin column using buffers designed to assist binding each component. (qiagen.com)
  • In molecular biology, bacterial DNA binding proteins are a family of small, usually basic proteins of about 90 residues that bind DNA and are known as histone-like proteins. (wikipedia.org)
  • Since bacterial binding proteins have a diversity of functions, it has been difficult to develop a common function for all of them. (wikipedia.org)
  • Eukaryotic histones package DNA to help it to fit in the nucleus, and they are known to be the most conserved proteins in nature. (wikipedia.org)
  • in these processes, bacterial DNA binding proteins have an architectural role, maintaining structural integrity as transcription, recombination, replication, or any other DNA-dependent process proceeds. (wikipedia.org)
  • Upon further investigation, it was discovered that the amino acid composition of HU resembles that of eukaryotic histones, thus prompting further research into the exact function of bacterial DNA binding proteins and discoveries of other related proteins in bacteria. (wikipedia.org)
  • Initially, bacterial DNA binding proteins were thought to help stabilize bacterial DNA. (wikipedia.org)
  • Currently, many more functions of bacteria DNA binding proteins have been discovered, including the regulation of gene expression by histone-like nucleoid-structuring protein, H-NS. (wikipedia.org)
  • However, it was thought that these domains would not be found in Mycoplasma, because its genome is so small and it only makes around 20 different DNA binding proteins responsible for organising the chromosome, compared to the hundreds made by other bacterial species. (blogspot.com)
  • The discovery suggests that this level of organisation and genetic control is common to all living cells, from the largest to the smallest, and can be achieved with little more than a handful of DNA binding proteins and the structural properties of the DNA itself. (blogspot.com)
  • CRISPR is a group of proteins and DNA that act as an immune system in some bacteria, vaccinating them with genetic memories of viral infections. (shiachat.com)
  • The RuvB proteins are thought to form hexameric rings on the exit points of the newly formed DNA duplexes, and it is proposed that they 'spool' the emerging DNA through the RuvA tetramer. (wikipedia.org)
  • RuvC proteins have been shown to form dimers in solution and its structure has been solved at 2.5A. It is thought to bind either on the open, DNA exposed face of a single RuvA tetramer, or to replace one of the two tetramers. (wikipedia.org)
  • However bacterial polysaccharides and lipopolysaccharides, and some polymeric proteins, can stimulate B lymphocytes without involvement of helper T cells. (wikipedia.org)
  • In E. coli DnaG associates through noncovalent interactions with bacterial replicative helicase DnaB to perform its primase activity, with three DnaG primase proteins associating with each DnaB helicase to form the primosome. (wikipedia.org)

gyrase and topoisomerase

  • Fleroxacin is a bactericidal drug that inhibits bacterial DNA gyrase and topoisomerase IV. (wikipedia.org)
  • Like other fluoroquinolones, enoxacin functions by inhibiting bacterial DNA gyrase and topoisomerase IV. (wikipedia.org)

bacteria by interfering with DNA

  • Like other quinolones and fluoroquinolones the compound eradicates bacteria by interfering with DNA replication (bacterial DNA replication, transcription, repair and recombination). (wikipedia.org)
  • Quinolones and fluoroquinolones are bactericidal drugs, eradicating bacteria by interfering with DNA replication. (wikipedia.org)

recombination

  • The protein has been implicated in DNA replication, recombination, and repair. (wikipedia.org)
  • Genetic transformation is the process by which a recipient bacterial cell takes up DNA from a neighboring cell and integrates this DNA into the recipient's genome by recombination. (wikipedia.org)
  • reported that in N. meningitidis DUSs occur at a significantly higher density in genes involved in DNA repair and recombination (as well as in restriction-modification and replication) than in other annotated gene groups. (wikipedia.org)
  • These authors proposed that the over-representation of DUS in DNA repair and recombination genes may reflect the benefit of maintaining the integrity of the DNA repair and recombination machinery by preferentially taking up genome maintenance genes that could replace their damaged counterparts in the recipient cell's genome. (wikipedia.org)
  • The inhibition of these enzymes prevents bacterial DNA replication, transcription, repair and recombination. (wikipedia.org)

polymerase

  • This domain is part of the beta subunit of bacterial DNA dependent RNA polymerase. (ebi.ac.uk)
  • the protein is involved in stabilizing the lagging strand as well as interacting with DNA polymerase III. (wikipedia.org)
  • The role of single-stranded DNA binding (SSB) protein during DNA replication in Escherichia coli cells has been studied, specifically the interactions between SSB and the χ subunit of DNA polymerase III in environments of varying salt concentrations. (wikipedia.org)
  • In DNA replication at the lagging strand site, DNA polymerase III removes nucleotides individually from the DNA binding protein. (wikipedia.org)
  • Research has shown that the ssDNA is stabilized by the interaction of SSB and the χ subunit of DNA polymerase III in E. coli, thus preparing for replication by maintaining the correct conformation that increases the binding affinity of enzymes to ssDNA. (wikipedia.org)
  • Furthermore, binding of SSB to DNA polymerase III at the replication fork prevents dissociation of SSB, consequently increasing the efficiency of DNA polymerase III to synthesize a new DNA strand. (wikipedia.org)
  • When H-NS is bound with RNA Polymerase to the promoter region, there are structural differences in the DNA that are accessible. (wikipedia.org)
  • A second structure was created through treatment of double stranded DNA from the polymerase chain reaction. (bl.uk)
  • These RNA oligonucleotides serve as primers, or starting points, for DNA synthesis by bacterial DNA polymerase III (Pol III). (wikipedia.org)
  • DnaG is important in bacterial DNA replication because DNA polymerase cannot initiate the synthesis of a DNA strand, but can only add nucleotides to a preexisting strand. (wikipedia.org)
  • DnaG catalyzes the synthesis of oligonucleotides in five discrete steps: template binding, nucleoside triphosphate (NTP) binding, initiation, extension to form a primer, and primer transfer to DNA polymerase III. (wikipedia.org)
  • This dinucleotide synthesis reaction is the same reaction as any other enzyme that catalyzes the formation of DNA or RNA (DNA Polymerase, RNA Polymerase), therefore DnaG must always synthesize oligonucleotides in the 5' to 3' direction. (wikipedia.org)
  • Once an oligonucleotide of appropriate length has been synthesized from the elongation step of primer synthesis, DnaG transfers the newly synthesized primer to DNA polymerase III for it to synthesize the DNA leading strand or Okazaki fragments for the lagging strand. (wikipedia.org)

chromosome organisation

  • They then used super-resolution microscopy to prove that this computer-generated map matched up with the real-life chromosome organisation inside bacterial cells. (blogspot.com)

topoisomerase

  • HU acts similarly to a histone by inducing negative supercoiling into circular DNA with the assistance of topoisomerase. (wikipedia.org)

single-stranded

  • In these high salt concentrations, the eukaryotic histone protein is eluted from a DNA solution in which single stranded DNA is bound covalently to cellulose. (wikipedia.org)
  • The ligase chain reaction was shown to produce DNA strands that could be incorporated into a structure with central base pairing and terminal single stranded DNA regions. (bl.uk)
  • CpG oligodeoxynucleotides (or CpG ODN) are short single-stranded synthetic DNA molecules that contain a cytosine triphosphate deoxynucleotide ("C") followed by a guanine triphosphate deoxynucleotide ("G"). The "p" refers to the phosphodiester link between consecutive nucleotides, although some ODN have a modified phosphorothioate (PS) backbone instead. (wikipedia.org)

Infections

  • There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. (mdpi.com)
  • The seriousness of the problem of bacterial resistance is confirmed by the number of deaths associated with drug-resistant bacterial infections-only in the EU it affects 25,000 people a year [ 1 ]. (mdpi.com)
  • Antimicrobial agents are important in the treatment of bacterial infections. (thefreelibrary.com)
  • Pathogenic strains of these bacteria are also an important cause of bacterial infections. (thefreelibrary.com)
  • Fleroxacin is effective in the treatment of a wide variety of infections, particularly uncomplicated cystitis in women, acute uncomplicated pyelonephritis, gonorrhea, bacterial enteritis, traveler's diarrhea, respiratory tract infections ( including exacerbation of chronic bronchitis). (wikipedia.org)
  • They are used in human and veterinary medicine to treat bacterial infections, as well as in animal husbandry. (wikipedia.org)

sequences

  • The researchers had previously shown that they could use CRISPR to store sequences of DNA in bacteria. (shiachat.com)
  • Iterons are directly repeated DNA sequences which play an important role in regulation of plasmid copy number in bacterial cells. (wikipedia.org)
  • Uptake signal sequence (USS) are short DNA sequences preferentially taken up by competent bacteria belonging to the Pasteurellaceae family (e.g. (wikipedia.org)

pathogens

  • Is the Subject Area "Bacterial pathogens" applicable to this article? (plos.org)
  • In: 4th Edition Virulence Mechanisms of Bacterial Pathogens. (ou.edu)

Escherichia

  • Escherichia coli (E. coli) is a Gram-negative bacterium and the main aerobic commensal bacterial species. (thefreelibrary.com)
  • A few organisms that have DnaG as their DNA primase are Escherichia coli (E. coli), Bacillus stearothermophilus, and Mycobacterium tuberculosis (MTB). (wikipedia.org)

species

  • Purified genomic DNA was isolated from various bacterial species using the GenElute Bacterial Genomic DNA kit. (sigmaaldrich.com)
  • This is a similar arrangement to that seen in some other larger bacterial species. (blogspot.com)
  • Up to nine taxa could be detected with commercially available DNA extraction kits while phenol-chloroform extraction lead to three detected species. (diva-portal.org)

strand

  • RuvA and RuvB bind to the four strand DNA structure formed in the Holliday junction intermediate, and migrate the strands through each other, using a putative spooling mechanism. (wikipedia.org)
  • The poly G tail found at either end of the DNA strand can vary in length and even number (Type D only have a poly G sequence on the 3'end), but its presence is critical to the activity of the molecule. (wikipedia.org)
  • This primer serves to prime leading strand DNA synthesis. (wikipedia.org)

detection

  • The goal of the current study is to evaluate the diagnostic value of plasma detection of bacterial DNA in ICU patients with a clinical suspicion of bacterial infection. (clinicaltrials.gov)
  • Depending on the methodology of DNA extraction, this phenomenon causes a shift in detection of microbial taxa in ecosystems and a possible misinterpretation of microbial interactions. (diva-portal.org)
  • Molecular detection of bacterial DNA is increasingly used. (wikipedia.org)

sequence

  • Next Generation Sequencing has allowed scientists to 'read' the DNA sequence of any genome, but this doesn't reveal how genetic information is managed and organised in the crowded and bustling biological environment inside a cell. (blogspot.com)
  • This research shows that the organisation and control of genes cannot be understood by just looking at the linear sequence of DNA in the genome. (blogspot.com)
  • There is an N-terminal Zinc-binding domain (residues 1-110) where a zinc ion is tetrahedrally coordinated between one histidine and three cysteine residues, which plays a role in recognizing sequence specific DNA binding sites. (wikipedia.org)

Antigens

  • If the ligand is a bacterial factor, the pathogen might be phagocytosed and digested, and its antigens presented to CD4+ T cells. (wikipedia.org)

resembles

  • HU is a small (10 kDa) bacterial histone-like protein that resembles the eukaryotic Histone H2B. (wikipedia.org)
  • Mitochondrial DNA resembles bacterial DNA. (wikipedia.org)

ligase

  • The researchers were interested in bacterial DNA ligase (LigA), which is essential for DNA replication, is highly conserved among numerous types of bacteria, and is quite different from its human counterpart. (blogspot.com)

helicase

  • The RuvAB complex can carry out DNA helicase activity, which helps unwind the duplex DNA. (wikipedia.org)
  • DnaG performs this catalysis near the replication fork that is formed by DnaB helicase during DNA replication. (wikipedia.org)

genomic

  • Sigma's GenElute Bacterial Genomic Kit provides a simple and convenient technique to isolate high quality DNA from both Gram negative ( Figure 1 ) and Gram positive bacteria ( Figure 2 ). (sigmaaldrich.com)
  • The GenElute Bacterial Genomic DNA Kit contains all of the reagents needed to purify genomic DNA from Gram negative bacteria ( Figure 3 ). (sigmaaldrich.com)
  • Typical DNA Yields with the GenElute Bacterial Genomic DNA Kit. (sigmaaldrich.com)
  • Comparison of Gram(-) bacteria genomic DNA isolation kits. (sigmaaldrich.com)
  • Agarose gel analysis of genomic DNA isolated from the indicated Gram negative bacteria prepared using the GenElute Bacterial Genomic DNA Kit versus kits from other suppliers. (sigmaaldrich.com)
  • Working together with colleagues in Spain, Japan and Australia, researchers led by Luis Serrano, ICREA research professor and leader of the Design of Biological Systems laboratory at the Centre for Genomic Regulation, focused their attention on the organisation of DNA within an organism with an extremely small genome -- the pneumonia pathogen Mycoplasma pneumoniae. (blogspot.com)
  • We are using functional genomics (genomic expression profiling using DNA arrays), together with more traditional approaches, to ask which E. coli genes are expressed in the large intestine. (ou.edu)

recombinant

  • Recombinant RecA production through bacterial induction produced soluble protein at a high yield. (bl.uk)
  • There are also recombinant variants which are created by inserting the DNA coding for it into bacteriae. (wikipedia.org)
  • The bacterial DNA is then called Recombinant DNA. (wikipedia.org)
  • This discovery was a cornerstone of the recombinant DNA revolution. (wikipedia.org)

transcription

  • This domain is the binding site for the antibacterial drug rifampin (and its analogues) which blocks the DNA/RNA tunnel and prevents initiation of transcription. (ebi.ac.uk)
  • H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription in bacteria by repressing and activating certain genes. (wikipedia.org)

strands

  • This work has produced DNA strands containing regions of base pair mismatching and with terminal three-way junctions. (bl.uk)
  • A method to create longer DNA strands with three-way junctions at the termini has also been developed. (bl.uk)
  • Single strands of DNA were produced that could be annealed to produce terminal three-way junctions. (bl.uk)
  • The binding of the RuvC protein to the RuvAB complex is thought to cleave the DNA strands, thereby resolving the Holliday junction. (wikipedia.org)
  • The enzyme DnaG, and any other DNA primase, synthesizes short strands of RNA known as oligonucleotides during DNA replication. (wikipedia.org)

junctions

  • With an α-helical hydrophobic core and two positively charged β-ribbon arms, HU binds non-specifically to dsDNA with low affinity but binds to altered DNA-such as junctions, nicks, gaps, forks, and overhangs-with high affinity. (wikipedia.org)
  • RuvA is a DNA-binding protein that binds Holliday junctions with high affinity. (wikipedia.org)

bacterium

  • When a virus infects a bacterium, CRISPR cuts out part of the foreign DNA and stores it in the bacteria's own genome. (shiachat.com)

Oligonucleotides

  • These oligonucleotides are known as primers because they act as a starting point for DNA synthesis. (wikipedia.org)
  • DnaG catalyzes the synthesis of oligonucleotides that are 10 to 60 nucleotides (the fundamental unit of DNA and RNA) long, however most of the oligonucleotides synthesized are 11 nucleotides. (wikipedia.org)

fluoroquinolones

  • However, for serious acute cases of pyelonephritis or bacterial prostatitis where the patient may need to be hospitalised, fluoroquinolones are recommended as first-line therapy. (wikipedia.org)

Fragment

  • Fragment approaches have been used successfully against several anti-bacterial targets (see for example here , here , and here ). (blogspot.com)

molecular biology

  • The Allprep Bacterial DNA/RNA/Protein Kit is intended for molecular biology applications. (qiagen.com)

researchers

  • Using a technique called Hi-C, which reveals the interactions between different pieces of DNA, the researchers created a three-dimensional 'map' of the Mycoplasma chromosome. (blogspot.com)
  • Researchers use DNA from ancient tooth tartar to chart changes in the bacterial communities that have lived in human mouths for 8,000 years. (the-scientist.com)
  • For starters, the researchers had to show that DNA can be used to encode not just genetic information, but any arbitrary sequential information into a genome. (shiachat.com)

elution

  • Following elution, the protein readily binds DNA, indicating the protein's high affinity for DNA. (wikipedia.org)

cell

  • Bacteria are first incubated with the appropriate enzymes to ensure efficient cell lysis and DNA release from the cells. (sigmaaldrich.com)
  • Many nanoparticles as well as naturally occurring clay minerals contain charged surfaces or edges that capture negatively charged DNA molecules after cell lysis within DNA extraction. (diva-portal.org)
  • How does all that DNA fit into the cell? (jobs.ac.uk)
  • Beta-lactamases target the peptidases of bacterial cell-wall biosynthetic process. (thefreelibrary.com)
  • According to findings of Professor Zsolt Balohh and his team at University of Newcastle (Australia), mitochondrial DNA is the leading cause of severe inflammation due to a massive amount of Mitochondrial DNA that leaks into the blood stream due to cell death into the blood stream of patients that survived major trauma. (wikipedia.org)
  • Since 1893, it has been recognized that Coley's toxin, a mixture of bacterial cell lysate, has immunostimulatory properties that could reduce the progression of some carcinomas, but it was not until 1983 that Tokunaga et al. (wikipedia.org)

structures

  • The potential of DNA structures in the field of materials science is hampered by current approaches to augmentation. (bl.uk)
  • This thesis will discuss the patterning of DNA structures with RecA. (bl.uk)
  • Two further DNA structures were produced on which patterning did not prove possible. (bl.uk)
  • In this paper, we describe how to combine several DNA extraction methods for the investigation of microbial community structures in clay. (diva-portal.org)

genes

  • DNA contains the instructions for life, encoded within genes. (blogspot.com)
  • Uptake of such genes could provide a mechanism for facilitating recovery from DNA damage after genotoxic stress. (wikipedia.org)

chromosomes

  • Within all cells, DNA is organised into very long lengths known as chromosomes. (blogspot.com)
  • Theta Type Replication: a type of bacterial DNA replication specific to circular chromosomes. (wikipedia.org)

cells

  • Notably, the CRG team, which counted with the expertise in Mycoplasma from the Serrano's laboratory and the collaboration of the ICREA research professor Marc Marti-Renom at CNAG-CRG, discovered that Mycoplasma's circular chromosome is consistently organised the same way in all the cells, with a region called the Origin (where DNA copying begins) at one end of the structure and the midpoint of the chromosome located at the opposite end. (blogspot.com)
  • For the first time, a primitive movie has been encoded in - and then played back from - DNA in living cells. (shiachat.com)
  • Other antibiotics were still toxic to the bacterial cells. (mdpi.com)

coli

  • We are comparing commensal (innocuous) E. coli strains with the pathogen E. coli O157:H7, for which we have fabricated DNA microarrays, to identify factors that allow the pathogen to infect normally colonized hosts. (ou.edu)

modification

  • The common bacterial base modification N6-methyladenine (m6A) is involved in many pathways related to an organism's ability to survive and interact with its environment. (nanoporetech.com)

binds

protein

  • DNA and RNA is ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. (qiagen.com)
  • The AllPrep Bacterial DNA/RNA/Protein Kit was previously sold by MO BIO under the same name. (qiagen.com)
  • The bacterial protein Recombinase A (RecA) may be able to provide this function. (bl.uk)
  • There was some evidence of DNA contamination and the purified protein showed low activity. (bl.uk)

bound

  • DNA is bound to the silica-based membrane and the remaining lysate is removed by centrifugation. (sigmaaldrich.com)

microbial

  • Synthetic CpG ODN differ from microbial DNA in that they have a partially or completely phosphorothioated (PS) backbone instead of the typical phosphodiester backbone and a poly G tail at the 3' end, 5' end, or both. (wikipedia.org)

bind

  • After binding DNA, DnaG must bind two NTPs in order to generate an enzyme-DNA-NTP-NTP quaternary complex. (wikipedia.org)

scientists

  • Scientists replay movie encoded in DNA "Molecular Recorder" would reveal secrets of brain development. (shiachat.com)
  • His discovery, while working in the laboratory of Paul Doty at Harvard University, that the denaturation of DNA was reversible (DNA hybridization) and depended on salt- and GC-content, had a major impact on how scientists thought about DNA, and how DNA could be handled in vitro. (wikipedia.org)

structural

  • The use of DNA as a structural material has been intensively developed since its inception in the early 1980s. (bl.uk)

affinity

  • Its crystal structure has been solved at 1.9A. RuvB is an ATPase that is only active in the presence of DNA and compared to RuvA, RuvB has a low affinity for DNA. (wikipedia.org)

Significant

  • Julius Marmur (March 22, 1926 - May 20, 1996, aged 70) was an American molecular biologist who made significant contributions to DNA research. (wikipedia.org)

extraction

  • A DNA extraction that comprises the DNA of all available taxa in an ecosystem is an essential step in population analysis, especially for next generation sequencing applications. (diva-portal.org)
  • Altogether, 13 different methods of commercially available DNA extraction kits provided by seven companies as well as the classical phenol-chloroform DNA extraction were compared. (diva-portal.org)

ecology

  • Filamentous Bacteriophage in Bio/Nano/Technology, Bacterial Pathogenesis and Ecology. (wikipedia.org)
  • Bacteriophage Ecology: Population Growth, Evolution, and Impact of Bacterial Viruses. (wikipedia.org)

environments

  • With recent demonstrations of nanopore sequencing in Antarctica and onboard the International Space Station, the ability to reliably characterize m6A presents an opportunity to further examine the role of methylation in bacterial adaptation to extreme environments. (nanoporetech.com)

repair

  • As such, RuvABC is critical to bacterial DNA repair. (wikipedia.org)

role

  • 2005. Role of the mucus layer in bacterial colonization of the intestine. (ou.edu)