No data available that match "DNA, Bacterial"



*  Bacterial DNA Extraction Kit: E. coli | Carolina.com
Demonstrates the DNA extraction process of freeze-dried E. coli cells. Cell walls are broken with a detergent, and the DNA is ... Bacterial DNA Extraction Kit: E. coli Class Kit Refill Item #154697 $44.95 ... Bacterial DNA Extraction Kit: E. coli. 2 Items *bvseo_sdk, java_sdk, bvseo-4.0.0 ... Bacterial DNA Extraction Kit: E. coli Class Kit Refill Item #154697 $44.95 ...
  https://www.carolina.com/dna-extraction/bacterial-dna-extraction-kit-e-coli/FAM_154696.pr
*  Nanopore detection of bacterial DNA base modifications
The common bacterial base modification N6-methyladenine (m6A) is involved in many pathways related to an organism's ability to ... Nanopore detection of bacterial DNA base modifications 13th April 2017 - BioRxiv. The common bacterial base modification N6- ... the ability to reliably characterize m6A presents an opportunity to further examine the role of methylation in bacterial ...
  https://nanoporetech.com/index.php/resource-centre/publications/nanopore-detection-bacterial-dna-base-modifications
*  Can arsenic bind to bacterial DNA? - Chemical Communications Blog
However, our study also suggested that arsenated DNA (As-DNA) is still less stable than normal DNA when hydrolysis is ... Normal' DNA has a backbone made of sugar and phosphate groups joined by phosphodiester linkages. Arsenic replaces the ... Now, scientists from the US and China say that arsenic substituted DNA may be more stable than first thought. ... Could Hydrolysis of Arsenic Substituted DNA be Prevented?: Protection Arises from Stacking Interactions. Jing Wang, Jiande Gu ...
  http://blogs.rsc.org/cc/2012/03/01/can-arsenic-bind-to-bacterial-dna/
*  AllPrep Bacterial DNA/RNA/Protein Kit - QIAGEN Online Shop
For the extraction of protein and nucleic acids from bacterial cell cultures ... Easy-to-use, the AllPrep Bacterial DNA/RNA/Protein Kit isolates total nucleic acids and cellular proteins from bacterial ... Home − Shop − Sample Technologies − RNA − Total RNA − Allprep Bacterial DNA/RNA/Protein Kit ... The Allprep Bacterial DNA/RNA/Protein Kit is intended for molecular biology applications. This product is not intended for the ...
  https://www.qiagen.com/at/shop/sample-technologies/rna/total-rna/allprep-bacterial-dna-rna-protein-kit/
*  DNA/RNA tunnel of bacterial DNA dependent RNA polymerase (IPR021975) | InterPro | EMBL-EBI
DNA/RNA tunnel of bacterial DNA dependent RNA polymerase (IPR021975). Short name: RNApol_Rpb2_rif ... This domain is part of the beta subunit of bacterial DNA dependent RNA polymerase. This domain is the binding site for the ... antibacterial drug rifampin (and its analogues) which blocks the DNA/RNA tunnel and prevents initiation of transcription. ...
  http://www.ebi.ac.uk/interpro/entry/IPR021975
*  Stochastic Self-Assembly of ParB Proteins Builds the Bacterial DNA Segregation Apparatus
In bacteria, the assembly mechanism of ParABS, the nucleoprotein super-complex that actively segregates the bacterial ... ParB forms a dynamic lattice with the DNA around parS.This assembly model and approach to characterizing large-scale, dynamic ... that nearly all ParB molecules are actively confined around parS by a network of synergistic protein-protein and protein-DNA ... Stochastic Self-Assembly of ParB Proteins Builds the Bacterial DNA Segregation Apparatus Aurore Sanchez 1 Diego Cattoni 2 Jean- ...
  https://hal.archives-ouvertes.fr/L2C/hal-01191677
*  Bacterial DNA May Integrate Into Human Genome More Readily in Tumor Tissue | NSF - National Science Foundation
They found that bacterial DNA was more likely to integrate in the genome in tumor samples than in normal, healthy somatic cells ... Bacterial DNA may integrate into the human genome more readily in tumors than in normal human tissue, scientists have found. ... Bacterial DNA May Integrate Into Human Genome More Readily in Tumor Tissue. ... The 3-D structure of the human genome, shown in a stretch of DNA inside a fractal globule.. Credit and Larger Version ...
  https://www.nsf.gov/news/news_summ.jsp?cntn_id=128258&org=ERE&from=news
*  Direct Detection Of Single-Nucleotide Polymorphisms In Bacterial DNA By SNPtrap
... Gronlund, Hugo; Moen, Birgitte; Hoorfar, ... Direct Detection Of Single-Nucleotide Polymorphisms In Bacterial DNA By SNPtrap}, url = {http://dx.doi.org/10.1080/ ...
  https://lup.lub.lu.se/search/publication/1918859
*  Bacterial DNA signatures in carotid atherosclerosis represent both commensals and pathogens of skin origin.
By using a novel subtraction broad-range PCR approach, we defined bacterial DNA signatures in surgically removed sterile ... DNA, Bacterial / isolation & purification*. DNA, Ribosomal. Female. Humans. Male. Metagenome*. Middle Aged. Polymerase Chain ... By using a novel subtraction broad-range PCR approach, we defined bacterial DNA signatures in surgically removed sterile ... Furthermore, 34 clones representing 21 bacterial sequence-types from the reagents used for DNA extraction and PCR amplification ...
  http://www.biomedsearch.com/nih/Bacterial-DNA-signatures-in-carotid/23406581.html
*  Bacterial DNA binding protein - Wikipedia
... bacterial DNA binding proteins were thought to help stabilize bacterial DNA. Currently, many more functions of bacteria DNA ... Research suggests that bacterial DNA binding protein has an important role during DNA replication; the protein is involved in ... The functions of bacterial DNA-binding proteins are not limited to DNA replication. Researchers have been investigating other ... These proteins participate in all DNA-dependent functions; in these processes, bacterial DNA binding proteins have an ...
  https://en.wikipedia.org/wiki/Bacterial_DNA_binding_protein
*  Bacterial Genomic DNA Extraction - Molecular Biology
Bacterial Genomic DNA Extraction - (Feb/16/2007 ). How do we make sure that it is only Genomic DNA and no PLASMID DNA that ... Excise the genomic DNA from the gel, extract the DNA by one of the old gel extraction protocol (do not use column, as the will ... A DNA prep which allows you to spool or hook the genomic DNA on a glass rod will allow you to wash small plasmid fragments, ... A different idea would be to gel purify your DNA. Assuming your plasmid is small (,7kb), you can gel purify your DNA on a low ...
  http://www.protocol-online.org/biology-forums/posts/24723.html
*  Terahertz spectroscopy for the isothermal detection of bacterial DNA by magnetic bead-based rolling circle amplification -...
... was developed for the isothermal detection of bacterial DNA. The synthetic bact ... The demand for rapid and sensitive bacterial detection is continuously increasing due to the significant requirements of ... Our results showed that 0.12 fmol of synthetic bacterial DNA and 0.05 ng μL−1 of genomic DNA could be effectively detected ... Terahertz spectroscopy for the isothermal detection of bacterial DNA by magnetic bead-based rolling circle amplification ...
  http://pubs.rsc.org/en/content/articlelanding/2017/an/c7an01438d
*  Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream...
Diagnostic Utility of Broad Range Bacterial 16S rRNA Gene PCR with Degradation of Human and Free Bacterial DNA in Bloodstream ... a biomarker of bacterial infection, can be used to exclude patients with a low probability of systemic bacterial infection. ... Infection Is More Sensitive Than an In-House Developed PCR without Degradation of Human and Free Bacterial DNA. Petra Rogina,1 ...
  https://www.hindawi.com/journals/mi/2014/108592/abs/
*  Onion or Bacterial Genomic DNA Isolation
Bacterial Genomic DNA Isolation. For 6 groups of 4-5 or 24-30 students.. $113.00 ... Genomic DNA is purified from a multitude of sources including mammalian tissue, such as cheek cells (BE-303), plant cells or ... Isolation of genomic DNA is an essential technique in modern research science, particularly molecular biology and biotechnology ... These kits use detergent lysis and precipitation to purify genomic DNA from onion or bacteria. Other plants or fruits can be ...
  https://www.gbiosciences.com/Onion-or-Bacterial-Genomic-DNA-Isolation
*  OC-034 Identifying Patients with Elevated Circulating Bacterial DNA May Reduce Infection and Death in Alcoholic Hepatitis | Gut
After unmasking, levels of bacterial DNA were then compared to clinical outcomes such as the development of infection within 7 ... We therefore sought to elucidate the impact of prednisolone in patients with elevated pre-treatment circulating bacterial DNA ( ... OC-034 Identifying Patients with Elevated Circulating Bacterial DNA May Reduce Infection and Death in Alcoholic Hepatitis ... OC-034 Identifying Patients with Elevated Circulating Bacterial DNA May Reduce Infection and Death in Alcoholic Hepatitis ...
  http://gut.bmj.com/content/65/Suppl_1/A20.2
*  Bacterial DNA May Integrate Into Human Genome More Readily in Tumor Tissue
... News Jun 27, 2013 ... Researchers Zoom in on DNA Code Being Read in Cells News Scientists have unveiled incredible images of how the DNA code is read ... They found that bacterial DNA was more likely to integrate in the genome in tumor samples than in normal, healthy somatic cells ... Bacterial DNA may integrate into the human genome more readily in tumors than in normal human tissue, scientists have found. ...
  https://www.technologynetworks.com/genomics/news/bacterial-dna-may-integrate-into-human-genome-more-readily-in-tumor-tissue-187681
*  Bacterial DNA activates cell mediated immune response and nitric oxide overproduction in peritoneal macrophages from patients...
Several studies have shown the immunogenic role of bacterial DNA in vitro, and we hypothesised that the presence of bacterial ... Cowdery JS, Chace JH, Yi AK, et al. Bacterial DNA induces NK cells to produce IFN-gamma in vivo and increases the toxicity of ... Interactions between bacterial CpG-DNA and TLR9 bridge innate and adaptive immunity. Curr Opin Microbiol2002;5:62-9. ... Sweet MJ, Stacey KJ, Kakuda DK, et al. IFN-gamma primes macrophage responses to bacterial DNA. J Interferon Cytokine Res1998;18 ...
  http://gut.bmj.com/content/53/6/860
*  DNA & RNA Purification Bacterial Genomic Miniprep Kit | China-Mainland | Sigma-Aldrich
The GenElute Bacterial Genomic Kit provides a simple and convenient technique to isolate high quality DNA from both Gram(-) and ... Figure 3. Purified genomic DNA was isolated from various bacterial species using the GenElute Bacterial Genomic DNA kit. A 1 µg ... Typical DNA Yields with the GenElute Bacterial Genomic DNA Kit.. Source. Type of Media. Amount of Overnight Culture. OD600 per ... The GenElute Bacterial Genomic DNA Kit contains all of the reagents needed to purify genomic DNA from Gram negative bacteria ( ...
  https://www.sigmaaldrich.com/china-mainland/zh/life-science/molecular-biology/dna-and-rna-purification/bacterial-genomic-miniprep-kit.html
*  "Periodontal bacterial-DNA initiated immuno-inflammatory responses in h" by Chebel Najib Bou
Bacterial DNA (bDNA) is one of the PAMPs mainly recognized by TLR9. Interaction of bDNA and its receptors leads to activation ... As expected, E.coli DNA, E.coli LPS and heat killed whole bacteria stimulated significantly increased cytokine production (p, ... Bacterial DNA (bDNA) is one of the PAMPs mainly recognized by TLR9. Interaction of bDNA and its receptors leads to activation ... As expected, E.coli DNA, E.coli LPS and heat killed whole bacteria stimulated significantly increased cytokine production (p. ...
  https://scholarscompass.vcu.edu/etd/97/
*  Scientists reveal hidden structures in bacterial DNA|Scientific India Magazine
Within all cells, DNA is organised into very long lengths known as chromosomes. ... DNA contains the instructions for life, encoded within genes. ... Within all cells, DNA is organised into very long lengths known ... DNA contains the instructions for life, encoded within genes. Within all cells, DNA is organised into very long lengths known ... and can be achieved with little more than a handful of DNA binding proteins and the structural properties of the DNA itself.. ...
  http://www.scind.org/538/Science/scientists-reveal-hidden-structures-in-bacterial-dna.html
*  A Magical Journey: Scientists reveal hidden structures in bacterial DNA
DNA contains the instructions for life, encoded within genes. Within all cells, DNA is organised into very long lengths known ... and can be achieved with little more than a handful of DNA binding proteins and the structural properties of the DNA itself.. ... This is a similar arrangement to that seen in some other larger bacterial species.. The scientists also used the Hi-C technique ... Using a technique called Hi-C, which reveals the interactions between different pieces of DNA, the researchers created a three- ...
  http://dannyboston.blogspot.com/2017/03/scientists-reveal-hidden-structures-in.html
*  Medical Laboratory and Biomedical Science: Bacterial DNA in The Human Genome
... the study group examined bacterial DNA integration into the human somatic genome. Here they present evidence that bacterial DNA ... Viral DNA is known to integrate in the human genome, but the integration of bacterial DNA has not been described. Using ... Hundreds of thousands of paired reads support random integration of Acinetobacter-like DNA in the human mitochondrial genome in ... There are 10× more bacterial cells in our bodies from the microbiome than human cells. ...
  http://clinical-laboratory.blogspot.fi/2013/11/bacterial-dna-in-human-genome.html
*  IUCr) Enzyme-adenylate structure of a bacterial ATP-dependent DNA ligase with a minimized DNA-binding surface
The DNA from the ChlV-Lig-DNA structure is shown in grey. (c) Surface of the Psy-Lig DNA-bound model (pink) superposed with the ... All bacterial genomes encode an essential NAD-dependent DNA ligase which joins Okazaki fragments during DNA replication, but ... enzyme is the first structure available for the minimal type of bacterial DNA ligases and is the smallest DNA ligase to be ... b) Psy-Lig modelled in the DNA-bound conformation by superposition of the Psy-Lig OB domain with the ChlV-Lig-DNA OB domain-DNA ...
  http://journals.iucr.org/d/issues/2014/11/00/rr5081/index.html
*  Practical Fragments: Fragments vs bacterial DNA ligase: triumph of structure-based design
The researchers were interested in bacterial DNA ligase (LigA), which is essential for DNA replication, is highly conserved ... Fragment approaches have been used successfully against several anti-bacterial targets (see for example here, here, and here). ...
  http://practicalfragments.blogspot.com/2014/02/fragments-vs-bacterial-dna-ligase.html
*  Bacterial Infection Diagnosis Using Blood DNA - Full Text View - ClinicalTrials.gov
Biospecimen Retention: Samples With DNA. Sample with bacterial DNA (and no human DNA will be studied) ... Bacterial Infection Diagnosis Using Blood DNA. The safety and scientific validity of this study is the responsibility of the ... The goal of the current study is to evaluate the diagnostic value of plasma detection of bacterial DNA in ICU patients with a ... Bacterial DNA Detection as a Diagnostic Tool of Infection in Critical Ill Patients With SIRS. ...
  https://clinicaltrials.gov/ct2/show/NCT00698919

No data available that match "DNA, Bacterial"



(1/38768) Detection of Chlamydia pneumoniae but not cytomegalovirus in occluded saphenous vein coronary artery bypass grafts.

BACKGROUND: A causal relation between atherosclerosis and chronic infection with Chlamydia pneumoniae and/or cytomegalovirus (CMV) has been suggested. Whether the unresolved problem of venous coronary artery bypass graft occlusion is related to infection with C pneumoniae and/or CMV has not been addressed. METHODS AND RESUTLS: Thirty-eight occluded coronary artery vein grafts and 20 native saphenous veins were examined. Detection of C pneumoniae DNA was performed by use of nested polymerase chain reaction (PCR). Homogenisates from the specimen were cultured for identification of viable C pneumoniae. Both conventional PCR and quantitative PCR for detection of CMV DNA were applied. Differential pathological changes (degree of inflammation, smooth muscle cell proliferation [MIB-1]) were determined and correlated to the detection of both microorganisms. C pneumoniae DNA could be detected in 25% of occluded vein grafts. Viable C pneumoniae was recovered from 16% of occluded vein grafts. Except for 1 native saphenous vein, all control vessels were negative for both C pneumoniae detection and culture. All pathological and control specimens were negative for CMV DNA detection. Pathological changes did not correlate with C pneumoniae detection. CONCLUSIONS: Occluded aorto-coronary venous grafts harbor C pneumoniae but not CMV. The detection of C pneumoniae in occluded vein grafts warrants further investigation.  (+info)

(2/38768) Acinetobacter bacteremia in Hong Kong: prospective study and review.

The epidemiological characteristics of 18 patients with acinetobacter bacteremia were analyzed. Patients (mean age, 55.5 years) developed bacteremia after an average of 14.1 days of hospitalization. Fifteen of 16 patients survived bacteremia caused by Acinetobacter baumannii. Cultures of blood from the remaining two patients yielded Acinetobacter lwoffii. Most patients (78%) resided in the general ward, while four patients (22%) were under intensive care. Genotyping by arbitrarily primed polymerase chain reaction analysis and the temporal sequence of isolation were more useful than phenotyping by antimicrobial susceptibility in the determination of the source of bacteremia, and the intravascular catheter was the leading infection source (39% of cases). The possibility of an association of glucose with the pathogenesis of acinetobacter infection was raised.  (+info)

(3/38768) Legionnaires' disease on a cruise ship linked to the water supply system: clinical and public health implications.

The occurrence of legionnaires' disease has been described previously in passengers of cruise ships, but determination of the source has been rare. A 67-year-old, male cigarette smoker with heart disease contracted legionnaires' disease during a cruise in September 1995 and died 9 days after disembarking. Legionella pneumophila serogroup 1 was isolated from the patient's sputum and the ship's water supply. Samples from the air-conditioning system were negative. L. pneumophila serogroup 1 isolates from the water supply matched the patient's isolate, by both monoclonal antibody subtyping and genomic fingerprinting. None of 116 crew members had significant antibody titers to L. pneumophila serogroup 1. One clinically suspected case of legionnaires' disease and one confirmed case were subsequently diagnosed among passengers cruising on the same ship in November 1995 and October 1996, respectively. This is the first documented evidence of the involvement of a water supply system in the transmission of legionella infection on ships. These cases were identified because of the presence of a unique international system of surveillance and collaboration between public health authorities.  (+info)

(4/38768) Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov.

A polyphasic taxonomic study was undertaken to clarify relationships within and between representative thermophilic alkalitolerant streptomycetes isolated from soil and appropriate marker strains. The resultant data, notably those from DNA-DNA relatedness studies, support the taxonomic integrity of the validly described species Streptomyces thermodiastaticus, Streptomyces thermoviolaceus and Streptomyces thermovulgaris. However, the genotypic and phenotypic data clearly show that Streptomyces thermonitrificans Desai and Dhala 1967 and S. thermovulgaris (Henssen 1957) Goodfellow et al. 1987 represent a single species. On the basis of priority, S. thermonitrificans is a later subjective synonym of S. thermovulgaris. Similarly, 10 out of the 11 representative thermophilic alkalitolerant isolates had a combination of properties consistent with their classification as S. thermovulgaris. The remaining thermophilic alkalitolerant isolate, Streptomyces strain TA56, merited species status. The name Streptomyces thermoalcalitolerans sp. nov. is proposed for this strain. A neutrophilic thermophilic isolate, Streptomyces strain NAR85, was identified as S. thermodiastaticus.  (+info)

(5/38768) Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively.

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.  (+info)

(6/38768) Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.

The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as 'biotype A' of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov. corresponds to [P.] haemolytica biogroups 3A-3H and the beta-glucosidase and meso-inositol-positive strains of [P.] haemolytica biogroup 9. All typable strains within M. glucosida belong to serotype 11. Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.] haemolytica biogroup 8D. Finally, Mannheimia varigena sp. nov. includes [P.] haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M. haemolytica), ATCC 49244T (M. granulomatis), CCUG 38457T = P925T (M. glucosida), CCUG 38470T = HPA92T (M. ruminalis) and CCUG 38462T = 177T (M. varigena).  (+info)

(7/38768) Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization.

The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA gyrase B subunit. The phylogenetic tree showed linkages between genomic species 1 (Acinetobacter calcoaceticus), 2 (Acinetobacter baumannii), 3 and TU13; genomic species 6, BJ15, BJ16 and BJ17; genomic species 5, BJ13 (synonym of TU14) and BJ14; genomic species 7 (Acinetobacter johnsonii), 10 and 11; and genomic species 8 and 9. The phylogenetic grouping of Acinetobacter strains based on gyrB genes was almost congruent with that based on DNA-DNA hybridization studies. Consequently, gyrB sequence comparison can be used to resolve the taxonomic positions of bacterial strains at the level of genomic species. However, minor discrepancies existed in the grouping of strains of genomic species 8, 9 and BJ17. The phylogenetic tree for these strains was reconstructed from the sequence of rpoD, the structural gene for the RNA polymerase sigma 70 factor. The latter tree was 100% congruent with the grouping based on DNA-DNA hybridization. The reliability of DNA-DNA hybridization may be superior to that of sequence comparison of a single protein-encoding gene in resolving closely related organisms since the former method measures the homologies between the nucleotide sequences of total genomic DNAs. Three strains that have not been characterized previously by DNA-DNA hybridization seem to belong to two new genomic species, one including strain ATCC 33308 and the other including strains ATCC 31012 and MBIC 1332.  (+info)

(8/38768) Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake.

Eight Gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic Ekho Lake (Vestfold Hills, East Antarctica). The cells contained storage granules and daughter cells could be motile. Bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. The strains tolerated a wide range of temperature, pH, concentrations of artificial seawater and NaCl, but had an absolute requirement for sodium ions. Glutamate was metabolized with and without an additional source of combined nitrogen. The dominant fatty acid was C18:1; other characteristic fatty acids were C18:2, C12:0 2-OH, C12:1 3-OH, C16:1, C16:0 and C18:0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C base composition was 62-64 mol%. 16S rRNA gene sequence comparisons showed that the isolates were phylogenetically close to the genera Antarctobacter, 'Marinosulfonomonas', Octadecabacter, Sagittula, Sulfitobacter and Roseobacter. Morphological, physiological and genotypic differences to these previously described and distinct genera support the description of a new genus and a new species, Roseovarius tolerans gen. nov., sp. nov. The type strain is EL-172T (= DSM 11457T).  (+info)



  • strand
  • RuvA and RuvB bind to the four strand DNA structure formed in the Holliday junction intermediate, and migrate the strands through each other, using a putative spooling mechanism. (wikipedia.org)
  • The poly G tail found at either end of the DNA strand can vary in length and even number (Type D only have a poly G sequence on the 3'end), but its presence is critical to the activity of the molecule. (wikipedia.org)
  • This primer serves to prime leading strand DNA synthesis. (wikipedia.org)
  • single-stranded
  • In these high salt concentrations, the eukaryotic histone protein is eluted from a DNA solution in which single stranded DNA is bound covalently to cellulose. (wikipedia.org)
  • CpG oligodeoxynucleotides (or CpG ODN) are short single-stranded synthetic DNA molecules that contain a cytosine triphosphate deoxynucleotide ("C") followed by a guanine triphosphate deoxynucleotide ("G"). The "p" refers to the phosphodiester link between consecutive nucleotides, although some ODN have a modified phosphorothioate (PS) backbone instead. (wikipedia.org)
  • Interactions
  • We found that nearly all ParB molecules are actively confined around parS by a network of synergistic protein-protein and protein-DNA interactions. (archives-ouvertes.fr)
  • We propose a new model for the formation of the ParABS partition complex based on nucleation and caging: ParB forms a dynamic lattice with the DNA around parS.This assembly model and approach to characterizing large-scale, dynamic interactions between macro-molecules may be generalizable to many unrelated machineries that self-assemble in superstructures. (archives-ouvertes.fr)
  • vitro
  • Several studies have shown the immunogenic role of bacterial DNA in vitro, and we hypothesised that the presence of bacterial DNA could activate the type I immune response in peritoneal macrophages from these patients, leading to greater cytokine synthesis (interleukin (IL)-2 and IL-12, tumour necrosis factor α, and interferon γ) and effector molecules such as nitric oxide. (bmj.com)
  • His discovery, while working in the laboratory of Paul Doty at Harvard University, that the denaturation of DNA was reversible (DNA hybridization) and depended on salt- and GC-content, had a major impact on how scientists thought about DNA, and how DNA could be handled in vitro. (wikipedia.org)
  • contaminants
  • After subtraction of these potential methodological contaminants, 23 bacterial sequence-types were considered as clinically relevant findings. (biomedsearch.com)
  • After washing to remove contaminants, the DNA is eluted with buffer into a collection tube. (sigmaaldrich.com)
  • genome
  • Bacterial DNA may integrate into the human genome more readily in tumors than in normal human tissue, scientists have found. (nsf.gov)
  • Scientists have already shown that bacteria can transfer DNA to the genome of an animal. (nsf.gov)
  • They found that bacterial DNA was more likely to integrate in the genome in tumor samples than in normal, healthy somatic cells. (nsf.gov)
  • The 3-D structure of the human genome, shown in a stretch of DNA inside a fractal globule. (nsf.gov)
  • Working together with colleagues in Spain, Japan and Australia, researchers led by Luis Serrano, ICREA research professor and leader of the Design of Biological Systems laboratory at the Centre for Genomic Regulation, focused their attention on the organisation of DNA within an organism with an extremely small genome - the pneumonia pathogen Mycoplasma pneumoniae . (scind.org)
  • Next Generation Sequencing has allowed scientists to 'read' the DNA sequence of any genome, but this doesn't reveal how genetic information is managed and organised in the crowded and bustling biological environment inside a cell. (scind.org)
  • This research shows that the organisation and control of genes cannot be understood by just looking at the linear sequence of DNA in the genome. (scind.org)
  • Oligonucleotides
  • The enzyme DnaG, and any other DNA primase, synthesizes short strands of RNA known as oligonucleotides during DNA replication. (wikipedia.org)
  • These oligonucleotides are known as primers because they act as a starting point for DNA synthesis. (wikipedia.org)
  • DnaG catalyzes the synthesis of oligonucleotides that are 10 to 60 nucleotides (the fundamental unit of DNA and RNA) long, however most of the oligonucleotides synthesized are 11 nucleotides. (wikipedia.org)
  • fluoroquinolones
  • However, for serious acute cases of pyelonephritis or bacterial prostatitis where the patient may need to be hospitalised, fluoroquinolones are recommended as first-line therapy. (wikipedia.org)
  • strands
  • The binding of the RuvC protein to the RuvAB complex is thought to cleave the DNA strands, thereby resolving the Holliday junction. (wikipedia.org)
  • infection
  • We assessed whether CD64 index, a biomarker of bacterial infection, can be used to exclude patients with a low probability of systemic bacterial infection. (hindawi.com)
  • After unmasking, levels of bacterial DNA were then compared to clinical outcomes such as the development of infection within 7 days, static and dynamic markers of liver function and mortality at 28 and 90 days. (bmj.com)
  • 2600 BC - Bacterial immunotherapy utilized by Egyptian pharaoh Imhotep who had a poultice, followed by incision, to facilitate the development of infection in the desired location and cause regression of the tumour. (wikipedia.org)
  • transcription
  • This domain is the binding site for the antibacterial drug rifampin (and its analogues) which blocks the DNA/RNA tunnel and prevents initiation of transcription. (ebi.ac.uk)
  • H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription in bacteria by repressing and activating certain genes. (wikipedia.org)
  • cell
  • Bacteria are first incubated with the appropriate enzymes to ensure efficient cell lysis and DNA release from the cells. (sigmaaldrich.com)
  • According to findings of Professor Zsolt Balohh and his team at University of Newcastle (Australia), mitochondrial DNA is the leading cause of severe inflammation due to a massive amount of Mitochondrial DNA that leaks into the blood stream due to cell death into the blood stream of patients that survived major trauma. (wikipedia.org)
  • Since 1893, it has been recognized that Coley's toxin, a mixture of bacterial cell lysate, has immunostimulatory properties that could reduce the progression of some carcinomas, but it was not until 1983 that Tokunaga et al. (wikipedia.org)
  • acute
  • Fleroxacin is effective in the treatment of a wide variety of infections, particularly uncomplicated cystitis in women, acute uncomplicated pyelonephritis, gonorrhea, bacterial enteritis, traveler's diarrhea, respiratory tract infections ( including exacerbation of chronic bronchitis). (wikipedia.org)
  • genes
  • DNA contains the instructions for life, encoded within genes. (scind.org)
  • Uptake of such genes could provide a mechanism for facilitating recovery from DNA damage after genotoxic stress. (wikipedia.org)
  • cells
  • Genomic DNA is purified from a multitude of sources including mammalian tissue, such as cheek cells (BE-303), plant cells or bacterial cells. (gbiosciences.com)
  • Within all cells, DNA is organised into very long lengths known as chromosomes. (scind.org)
  • If the ligand is a bacterial factor, the pathogen might be phagocytosed and digested, and its antigens presented to CD4+ T cells. (wikipedia.org)
  • scientists
  • Now, scientists from the US and China say that arsenic substituted DNA may be more stable than first thought. (rsc.org)
  • Scientists have unveiled incredible images of how the DNA code is read and interpreted - revealing new detail about one of the fundamental processes of life. (technologynetworks.com)
  • residues
  • There is an N-terminal Zinc-binding domain (residues 1-110) where a zinc ion is tetrahedrally coordinated between one histidine and three cysteine residues, which plays a role in recognizing sequence specific DNA binding sites. (wikipedia.org)
  • synthetic
  • Our results showed that 0.12 fmol of synthetic bacterial DNA and 0.05 ng μL −1 of genomic DNA could be effectively detected using this assay. (rsc.org)
  • demonstrated that the CpG motif within bacterial DNA was responsible for the immunostimulatory effects and developed synthetic CpG ODN. (wikipedia.org)
  • Synthetic CpG ODN differ from microbial DNA in that they have a partially or completely phosphorothioated (PS) backbone instead of the typical phosphodiester backbone and a poly G tail at the 3' end, 5' end, or both. (wikipedia.org)
  • junctions
  • With an α-helical hydrophobic core and two positively charged β-ribbon arms, HU binds non-specifically to dsDNA with low affinity but binds to altered DNA-such as junctions, nicks, gaps, forks, and overhangs-with high affinity. (wikipedia.org)
  • RuvA is a DNA-binding protein that binds Holliday junctions with high affinity. (wikipedia.org)
  • bound
  • DNA is bound to the silica-based membrane and the remaining lysate is removed by centrifugation. (sigmaaldrich.com)
  • signatures
  • By using a novel subtraction broad-range PCR approach, we defined bacterial DNA signatures in surgically removed sterile carotid artery endarterectomy plaques of patients with carotid atherosclerosis. (biomedsearch.com)
  • significantly
  • The ability of peritoneal macrophages to synthesise nitric oxide and levels of all cytokines were significantly increased in patients with bacterial DNA. (bmj.com)
  • affinity
  • Its crystal structure has been solved at 1.9A. RuvB is an ATPase that is only active in the presence of DNA and compared to RuvA, RuvB has a low affinity for DNA. (wikipedia.org)
  • molecules
  • As DNA molecules are much less absorptive than water molecules in the THz range, the RCA products on the surface of the MBs cause a significant decrease in THz absorption, which can be sensitively probed by THz spectroscopy. (rsc.org)
  • role
  • With recent demonstrations of nanopore sequencing in Antarctica and onboard the International Space Station, the ability to reliably characterize m6A presents an opportunity to further examine the role of methylation in bacterial adaptation to extreme environments. (nanoporetech.com)
  • The phenomenon might play a role in cancer and other diseases associated with DNA damage. (nsf.gov)
  • protein
  • DNA and RNA is ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. (qiagen.com)
  • The AllPrep Bacterial DNA/RNA/Protein Kit was previously sold by MO BIO under the same name. (qiagen.com)
  • specific
  • The proposed strategy not only represents a new method for the isothermal detection of the target bacterial DNA but also provides a general methodology for sensitive and specific DNA biosensing using THz spectroscopy. (rsc.org)