The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae. (1/8779)

Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5'-->3' Exo1 and the 3'-->5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) mutants defective in 3'-->5' proofreading exonuclease, mutants defective in the 5'-->3' exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes.  (+info)

Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. (2/8779)

Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.  (+info)

Histone octamer transfer by a chromatin-remodeling complex. (3/8779)

RSC, an abundant, essential chromatin-remodeling complex related to SWI/SNF complex, catalyzes the transfer of a histone octamer from a nucleosome core particle to naked DNA. The newly formed octamer-DNA complex is identical with a nucleosome in all respects. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. The mechanism may entail formation of a duplex displacement loop on the nucleosome, facilitating the entry of exogeneous DNA and the release of the endogenous molecule.  (+info)

Regulation of the start of DNA replication in Schizosaccharomyces pombe. (4/8779)

Cells of Schizosaccharomyces pombe were grown in minimal medium with different nitrogen sources under steady-state conditions, with doubling times ranging from 2.5 to 14 hours. Flow cytometry and fluorescence microscopy confirmed earlier findings that at rapid growth rates, the G1 phase was short and cell separation occurred at the end of S phase. For some nitrogen sources, the growth rate was greatly decreased, the G1 phase occupied 30-50% of the cell cycle, and cell separation occurred in early G1. In contrast, other nitrogen sources supported low growth rates without any significant increase in G1 duration. The method described allows manipulation of the length of G1 and the relative cell cycle position of S phase in wild-type cells. Cell mass was measured by flow cytometry as scattered light and as protein-associated fluorescence. The extensions of G1 were not related to cell mass at entry into S phase. Our data do not support the hypothesis that the cells must reach a certain fixed, critical mass before entry into S. We suggest that cell mass at the G1/S transition point is variable and determined by a set of molecular parameters. In the present experiments, these parameters were influenced by the different nitrogen sources in a way that was independent of the actual growth rate.  (+info)

The prokaryotic beta-recombinase catalyzes site-specific recombination in mammalian cells. (5/8779)

The development of new strategies for the in vivo modification of eukaryotic genomes has become an important objective of current research. Site-specific recombination has proven useful, as it allows controlled manipulation of murine, plant, and yeast genomes. Here we provide the first evidence that the prokaryotic site-specific recombinase (beta-recombinase), which catalyzes only intramolecular recombination, is active in eukaryotic environments. beta-Recombinase, encoded by the beta gene of the Gram-positive broad host range plasmid pSM19035, has been functionally expressed in eukaryotic cell lines, demonstrating high avidity for the nuclear compartment and forming a clear speckled pattern when assayed by indirect immunofluorescence. In simian COS-1 cells, transient beta-recombinase expression promoted deletion of a DNA fragment lying between two directly oriented specific recognition/crossing over sequences (six sites) located as an extrachromosomal DNA substrate. The same result was obtained in a recombination-dependent lacZ activation system tested in a cell line that stably expresses the beta-recombinase protein. In stable NIH/3T3 clones bearing different number of copies of the target sequences integrated at distinct chromosomal locations, transient beta-recombinase expression also promoted deletion of the intervening DNA, independently of the insertion position of the target sequences. The utility of this new recombination tool for the manipulation of eukaryotic genomes, used either alone or in combination with the other recombination systems currently in use, is discussed.  (+info)

The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance. (6/8779)

The mdm17 mutation causes temperature-dependent defects in mitochondrial inheritance, mitochondrial morphology, and the maintenance of mitochondrial DNA in the yeast Saccharomyces cerevisiae. Defects in mitochondrial transmission to daughter buds and changes in mitochondrial morphology were apparent within 30 min after shifting cells to 37 degrees C, while loss of the mitochondrial genome occurred after 4-24 h at the elevated temperature. The mdm17 lesion mapped to MGM1, a gene encoding a dynamin-like GTPase previously implicated in mitochondrial genome maintenance, and the cloned MGM1 gene complements all of the mdm17 mutant phenotypes. Cells with an mgm1-null mutation displayed aberrant mitochondrial inheritance and morphology. A version of mgm1 mutated in a conserved residue in the putative GTP-binding site was unable to complement any of the mutant defects. It also caused aberrant mitochondrial distribution and morphology when expressed at high levels in cells that also contained a wild-type copy of the gene. Mgm1p was localized to the mitochondrial outer membrane and fractionated as a component of a high molecular weight complex. These results indicate that Mgm1p is a mitochondrial inheritance and morphology component that functions on the mitochondrial surface.  (+info)

All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature. (7/8779)

All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned. These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature. The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs. Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex.  (+info)

The nuclear ribosomal DNA intergenic spacer as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on pinus root systems. (8/8779)

Polymorphism of the nuclear ribosomal DNA intergenic spacer (IGS) of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was studied to evaluate whether this sequence could be used in field studies to estimate the diversity of strains forming mycorrhizas on individual Pinus pinaster root systems. This sequence was amplified by PCR from 125 haploid homokaryotic strains collected in 14 P. pinaster stands along the Atlantic coast of France by using conserved oligonucleotide primers. Restriction enzyme digestion of the amplified 3.4-kbp-long IGS allowed us to characterize 24 alleles whose frequencies differed. Nine of these alleles were found only once, whereas about 60% of the strains contained four of the alleles. Local populations could be almost as diverse as the entire population along a 150-km stretch of coastline that was examined; for example, 13 alleles were found in a single forest stand. The IGS from one strain was partially sequenced, and the sequence data were used to design oligonucleotides which allowed separate PCR amplification of three different segments of the IGS. Most polymorphisms observed among the full-length IGS regions resulted from polymorphisms in an internal ca. 1,500-bp-long sequence characterized by length variations that may have resulted from variable numbers of a T2AG3 motif. This internal polymorphic sequence could not be amplified from the genomes of nine other Hebeloma species. Analysis of this internal sequence amplified from the haploid progenies of 10 fruiting bodies collected in a 70-m2 area resulted in identification of six allelic forms and seven distinct diplotypes out of the 21 possible different combinations. Moreover, optimization of the PCR conditions resulted in amplification of this sequence from more than 80% of the DNA samples extracted from individual H. cylindrosporum infected P. pinaster mycorrhizal root tips, thus demonstrating the usefulness of this sequence for studying the below-ground diversity of mycorrhizas formed by genets belonging to the same fungal species.  (+info)

DNA barcoding Microbial DNA barcoding Pollen DNA barcoding DNA barcoding in diet assessment Consortium for the Barcode of Life ... as primary fungal DNA barcode, the necessity of establishing a second DNA barcode marker was expressed. Several attempts were ... Fungal DNA barcoding can help to identify and associate anamorphic and teleomorphic stages of fungi, and through that to reduce ... Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and ...
... and to collect and document fungi for DNA barcoding. Fungal Diversity Survey, Inc. is a Charitable 501(c)(3) organization ... Fungi Magazine, 101, pp.28-36 FunDiS , Fungal Diversity Survey Fungal Diversity Database on iNaturalist (Accuracy disputes from ... Fungal Diversity Database - Build a database of fungal observations on iNaturalist of sufficient scale and quality to be useful ... Fungal Diversity Survey, or FunDiS, is a nonprofit citizen science organization formerly known as North American Mycoflora ...
Alper, I; Michel, F; Labrie, S (2011). "Ribosomal DNA Polymorphisms in the Yeast Geotrichum candidum". Fungal Biology. 115 (12 ... Fungal plant pathogens and diseases, Fungal citrus diseases, Animal fungal diseases, Organisms breaking down plastic, Fungi ... Fungal growth can be supported by D-glucose, D-mannose, D-xylose, L-sorbose, D-fructose, D-galactose, sucrose, D-mannitol, D- ...
All species form agaricoid basidiocarps (gilled mushrooms). The family is based on recent DNA research. Vizzini A, Consiglio G ... nov., and Pleurocollybia incorporated into Callistosporium". Fungal Diversity. 101: 211-259. doi:10.1007/s13225-020-00441-x. v ...
... based on nuclear ribosomal DNA". Fungal Biology. 115: 381-392. doi:10.1016/j.funbio.2011.02.004. 6. Allomyces. Phillip W. ...
... based on nuclear ribosomal DNA". Fungal Biology. 115 (4): 381-392. doi:10.1016/j.funbio.2011.02.004. ISSN 1878-6146. PMID ...
DNA replication occurs when one double-stranded DNA molecule creates two single strands of DNA, each of which is a template for ... Because RIP is so efficient at detecting and mutating repeats, fungal biologists often use it as a tool for mutagenesis. A ... These reactants can lead to both single-stranded DNA breaks and double-stranded DNA breaks. Third, bonds in DNA eventually ... It is a DNA methyltransferease-like protein, that when mutated or knocked out results in loss of RIP. Deletion of the rid ...
As a result of how the DNA samples were processed, about 80 percent of the reference genome came from eight people and one male ... "1000 Fungal Genomes Project". mycocosm.jgi.doe.gov. Retrieved 2022-08-18. Genome Reference Consortium (Genome projects, ... As they are assembled from the sequencing of DNA from a number of individual donors, reference genomes do not accurately ... Instead a reference provides a haploid mosaic of different DNA sequences from each donor. For example, one the most recent ...
... morphological and multilocus DNA sequence analyses". Fungal Diversity. 58 (1): 73-101. doi:10.1007/s13225-012-0184-7. Neves MA ... Fungal Diversity. 55 (1): 109-123. doi:10.1007/s13225-012-0154-0. "Phylloporus on Boletineae: Surveys and Revisions". " ...
... morphological and multilocus DNA sequence analyses". Fungal Diversity. 58: 73-101. doi:10.1007/s13225-012-0184-7. S2CID ... Fungal Diversity. 55 (1): 109-23. doi:10.1007/s13225-012-0154-0. S2CID 17272328. Zheng NK, Tang LP, Li YC, Tolgor B, Zhu XT, ...
Molecular phylogeny of the Blastocladiomycota (Fungi) based on nuclear ribosomal DNA. Fungal Biology 115:381-392. Kendrick, ... Fungal Diversity. 90 (1): 135-159. doi:10.1007/s13225-018-0401-0. Hibbett DS et al. 2007. A higher-level phylogenetic ...
Molecular phylogeny of the Blastocladiomycota (Fungi) based on nuclear ribosomal DNA. Fungal Biology 115: 381-392. James, T.Y ...
2005). "Structural analysis of epolactaene derivatives as DNA polymerase inhibitors and anti-inflammatory compounds". Int J Mol ... The fungal isolate ergothioneine is actively absorbed and concentrated by the human body via SLC22A4. Other notable fungal ... The statins lovastatin, mevastatin, and simvastatin precursor monacolin J, are fungal isolates. Additional fungal isolates that ... are antibiotic fungal isolates. Antibiotics retapamulin, tiamulin, and valnemulin are derivatives of the fungal isolate ...
This species is part of the community of fungal successors of decaying wood. A Finnish study found that it fruited most ... "Phylogenetic relationships in European Ceriporiopsis species inferred from nuclear and mitochondrial ribosomal DNA sequences". ... Yu, Chang-Jun; Zuo, Li; Dai, Yu-Cheng (2005). "Three polypores from Xizang new to China" (PDF). Fungal Science. 20 (3-4): 61-68 ... "Skeletocutis nothofagi Rajchenb". The Global Fungal Red List Initiative. IUCN. Retrieved 30 September 2016. Fries, Elias Magnus ...
"Phylogeny of the genus Agaricus inferred from restriction analysis of enzymatically amplified ribosomal DNA". Fungal Genet Biol ... Based on DNA analysis of ITS1, ITS2, and 5.8S sequences, the studied species of this section could be divided into six distinct ... The fungal genus Agaricus as late as 2008 was believed to contain about 200 species worldwide but since then, molecular ...
in Fungal Diversity vol.90, Issue 1 on page 151 in 2018. It is parasitic. But can grow solitary on agar media, thus cold ... based on nuclear small subunit ribosomal DNA sequences". Mol. Phylogenet. Evol. 16 (2): 253-62. doi:10.1006/mpev.2000.0775. ... Fungal Diversity. 90 (1): 135-159. doi:10.1007/s13225-018-0401-0. Tanabe Y, O'Donnell K, Saikawa M, Sugiyama J (August 2000). " ...
Li GJ, Hyde KD, Zhao RL, Hongsanan S, Abdel-Aziz FA (2016). "Fungal diversity notes 253-366" (PDF). Fungal Divers. 78. doi: ... Molecular research, based on cladistic analysis of DNA sequences, suggests the genus may be polyphyletic. The type species has ...
Ochricompactae" (PDF). Fungal Diversity. 28: 15-40. Eberhardt U, Verbeken A (2004). "Sequestrate Lactarius species from ... Russulales; Basidiomycota) in Alaska, based on soil and sporocarp DNA" (PDF). Molecular Ecology. 18 (10): 2213-2227. doi: ... Rinaldi AC, Comandini O, Kuyper TW (2008). "Ectomycorrhizal fungal diversity: separating the wheat from the chaff" (PDF). ... Twieg BD, Durall DM, Simard SW (2007). "Ectomycorrhizal fungal succession in mixed temperate forests". New Phytologist. 176 (2 ...
DNA sequencing data reveals the presence of two major groups representing cryptic species in F. subglutinans. These were ... Fusarium subglutinans is a fungal plant pathogen. Fusarium subglutinans is the anamorph of Gibberella fujikuroi. Fusarium ... Fungal plant pathogens and diseases, Maize diseases, Mango tree diseases, Fungi described in 1925, All stub articles, ...
"Family placement of Ascotaiwania and Ascolacicola based on DNA sequences from the large subunit rRNA gene". Fungal Divers. 2: ... Fungal Divers. 35: 1-187. E. B. Gareth Jones and Ka-Lai Pang (Editors) Marine Fungi: and Fungal-like Organisms (2012), p. 37, ... Lastly, anamorphic fungal genus Monotosporella was also added later to the order and family. Generally, Savoryellaceae species ... doi:10.1016/S0007-1536(69)80169-5. Tsui, C. K. M.; Hyde, K. D. (2003). "Freshwater mycology". Fungal Divers. Res. Ser. 10: 1- ...
... or DNA-based tests." Pathogenesis of the fungal contaminants include a wide range of factors such as "invasiveness, ... The issues of fungal identification observed as mentioned in this study do not pertain only to fungal contamination of contact ... "fungal strain isolated in culture." In one instance, a fungal strain previously identified as Arthrobotrys oligospora in a ... is amphotericin B which is a specific anti-fungal drug. For the treatment for filamentous fungal keratitis, "topical natamycin ...
Fungal spores found in the body of the nail resembled the characteristic morphology of N. sphaerica. DNA sequence analysis ... Fungal spores trapped on the agar strips were developed and counted. They were then cultured into isolates allowing for ... A woman in south India was diagnosed with a fungal corneal ulcer after being hit in the eye from a cow's tail. Analysis of ... It was hypothesized that this special case of fungal corneal ulcer was caused by transfer of spores to the patients eye from ...
Vánky, K. (2001). "The emended Ustilaginaceae of the modern classificatory system for smut fimgi". Fungal Diversity. 6: 131-147 ... "Phylogenetic studies on nuclear large subunit ribosomal DNA sequences of smut fungi and related taxa". Canadian Journal of ...
Lücking, Robert; Nadel, Miko; Araujo, Elena; Gerlach, Alice (2020). "Two decades of DNA barcoding in the genus Usnea ( ... Fungal Diversity. 84 (1): 101-117. doi:10.1007/s13225-017-0379-z. S2CID 256071368. Ohmura 2001, p. 8. Ohmura 2001, p. 9. Ohmura ... Parmeliaceae): how useful and reliable is the ITS?". Plant and Fungal Systematics. 65 (2): 303-357. doi:10.35535/pfsyst-2020- ...
DNA analysis has shown this belongs within the Agaricales. Several other species formerly referred to Myriococcum are now ... Koukol O (2016). "Myriococcum revisited: a revision of an overlooked fungal genus". Plant Systematics and Evolution. 302 (8): ...
... is an N-terminal domain involved in copper-dependent DNA binding. It is named for its resemblance to a fist. It can ... be found in some fungal transcription factors. These proteins activate the transcription of the metallothionein gene in ... which allows DNA binding. Copper fist definition Turner RB, Smith DL, Zawrotny ME, Summers MF, Posewitz MC, Winge DR (July 1998 ...
Although the replication and transcription of DNA is highly standardized in eukaryotes, the same cannot be said for their ... Besides plants and animals, the evolutionary history of various fungal species is dotted by past and recent whole-genome ... Lidzbarsky GA, Shkolnik T, Nevo E (June 2009). Idnurm A (ed.). "Adaptive response to DNA-damaging agents in natural ... April 2009). "Genome sequence of the lager brewing yeast, an interspecies hybrid". DNA Research. 16 (2): 115-129. doi:10.1093/ ...
Fungal Genet. Biol. 43 (7): 511-29. doi:10.1016/j.fgb.2006.02.006. PMID 16626980. S2CID 43275127. Chandelier A, Abras S, ... "Genetic characterization of the natural hybrid species Phytophthora alni as inferred from nuclear and mitochondrial DNA ...
"Fungal diversity notes 603-708: taxonomic and phylogenetic notes on genera and species". Fungal Diversity. 87 (1): 1-235. doi: ... 2015) introduced P. garethjonesii based on DNA sequence data and morphology and placed this species in Melanommataceae in ... Fungal Diversity. 87 (2): 237-298. doi:10.1007/s13225-017-0389-x. S2CID 256070993. Tsui, C. K. M.; Berbee, M. L. (2006). " ... a fungal infection of the cornea), as well as Pleurothecium recurvatum (formerly Carpoligna pleurothecii). A multi-locus ...
The species was formerly placed in the genus Clitocybe, but has been separated on DNA characteristics. Vizzini A, Consiglio G, ... nov., and Pleurocollybia incorporated into Callistosporium". Fungal Diversity. 101: 211-259. doi:10.1007/s13225-020-00441-x. ...
Suggested measures of overall genome related indices in yeast include ANI, digital DNA-DNA hybridisation (dDDH) and Kr distance ... Fungal genomes are among the smallest genomes of eukaryotes. The sizes of fungal genomes range from less than 10 Mbp to ... The comparison of fungal genomes has been used to study the evolution of fungi, to improve the resolution of the phylogeny of ... Due to their compact size fungal genomes can be sequenced with less resources than most other eukaryotic genomes and are thus ...
... and mit-atp6-DNA combined sequences". Fungal Biology. 114 (2-3): 224-34. doi:10.1016/j.funbio.2010.01.002. hdl:11336/15813. ...
DNA barcoding Microbial DNA barcoding Pollen DNA barcoding DNA barcoding in diet assessment Consortium for the Barcode of Life ... as primary fungal DNA barcode, the necessity of establishing a second DNA barcode marker was expressed. Several attempts were ... Fungal DNA barcoding can help to identify and associate anamorphic and teleomorphic stages of fungi, and through that to reduce ... Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and ...
... reports detecting fungal DNA or cells-typically at low abundance-in 35 different cancer types, with fungal species composition ... Fungal DNA, Cells Found in Human Tumors. Its not clear whether, or how, the organisms might play a role in disease pathology. ... Fungal DNA is present in various types of cancer, according to two studies published yesterday (September 29) in Cell. The ... Across various sites, "several Candida species were enriched in tumor samples and tumor-associated Candida DNA was predictive ...
Thus, having an efficient species-level identification system could significantly enhance our ability to treat fungal diseases ... the history of DNA sequence-based fungal identification; (ii) the emergence of the ITS region as the consensus primary fungal ... Database establishment for the secondary fungal DNA barcode translational elongation factor 1α (TEF1α) 1. Meyer W, Irinyi L, ... DNA barcoding is a potent approach for rapid identification of fungal specimens, generating novel species hypothesis, and ...
DNA, Fungal Fungi Genes, RRNA Mycological Typing Techniques Polymerase Chain Reaction Sequence Analysis, DNA ... Assessment of fungal diversity in a water-damaged office building Cite CITE. Title : Assessment of fungal diversity in a water- ... Title : Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing Personal Author(s) : ... Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing. ...
An increase in pediatric opportunistic fungal infections in hospitals means greater attention must be paid to this problem. ... Detection of Fungal DNA. Polymerase chain reaction (PCR) may be a powerful tool for early diagnosis of IFIs.[23] No studies ... Modern imaging studies, detection of circulating fungal cell wall components and DNA in blood and other body fluids may enhance ... The beta-D glucan assay has been studied in adult patients with fungal infections.[22] Due to the presence of (1,3)-ß-D-glucan ...
CDC has developed a novel test using PCR and DNA sequencing to detect fungal DNA in cerebrospinal fluid (CSF), other body ... CDCs fungal PCR test At-A-Glance. *Limit of detection: 1 picogram DNA per ml ... A negative fungal culture or negative fungal polymerase chain reaction (PCR) test from a diagnostic specimen obtained from CSF ... CDCs PCR test for detection of fungal DNA ... Fungal Meningitis For patients with fungal meningitis, what are ...
Introduction The risk for invasive fungal infections is high in very low birth weight (VLBW) infants (< 1500 g) and highest for ... Examples include PCR and DNA microarray technology. The hope is that these techniques will allow for the rapid detection of ... Culture (aerobic or fungal). Culture (aerobic or fungal) or biopsy. Fungal culture or biopsy. ... Culture (aerobic or fungal). Culture (aerobic or fungal) or biopsy. Fungal culture or biopsy. ...
In conclusion, this study suggests that different fungal structures appeared when different green manures were applied, which ... have different fungal structures, despite the soil originally being the same. Moreover, some isolated strains showed plant- ... DNA was extracted from the isolated fungal strains using the ZR bacterial/fungal DNA MiniPrep KitTM (Zymo Research Corp., ... The abundance of total fungal biomass was assessed based on the number of colony-forming units (CFUs). The fungal biomass was ...
... including a fungal isolate recovered by FDA from a BBG vial and fungal DNA isolated by an outside laboratory from a vitreous ... Fungal isolates were identified by morphologic and DNA sequence analysis (15). Fusarium isolates were compared by multilocus ... fungal infections are rare (5-7). The clinical course of fungal endophthalmitis is frequently prolonged and is associated with ... Exogenous fungal endophthalmitis: microbiology and clinical outcomes. Ophthalmology. 1507;2008:1501-7 .DOIPubMedGoogle Scholar ...
The solution structure of a complex between the DNA binding domain of a fungal GATA factor and a 13 base-pair oligonucleotide ... The solution structure of a complex between the DNA binding domain of a fungal GATA factor and a 13 base-pair oligonucleotide ... The solution structure of a fungal AREA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA ... The solution structure of a fungal AREA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA ...
Periplaneta americana acts as a potential mechanical vector of medically important fungal pathogens from inside sewer systems ... Evaluation of DNA quality and quantity. Listen The quality and quantity of DNA were assessed and predicted using a Nanodrop ... DNA extraction. Listen Various methods have been described for DNA extraction from fungi. We used a modified CTAB protocol, ... PCR and DNA sequencing. Listen The extracted DNA was amplified using the ITS1 (5′TCCGTAGGTGAACCTGCGG3′) and ITS4 (5′ ...
The effect of antifungal antibiotics on fungal DNA. DNA, FUNGAL / *drug eff. ANTIBIOTICS, ANTIFUNGAL / *pharmacol /radiation ...
DNA Repair / drug effects* * DNA Repair / genetics * DNA, Fungal / drug effects * DNA, Fungal / genetics ... These DNA repair pathways are highly conserved between yeast, DT40, and humans. We propose that in humans, mutagenic TLS is ... The role of TLS DNA repair was further confirmed by functional profiling in DT40 avian cell lines, but also revealed that TLS ... and homologous recombination DNA repair likely play competing roles in cellular susceptibility to TCE metabolites in higher ...
Genetics vs Epigenetics: A Deep Dive into Robbs DNA , THRR170. High Blood Pressure, Lifting Philosophy, Fungal Rash , THRR169 ...
April 13, 2022 - Visualizing DNA Damage Through a Molecular Lens. * April 15, 2022 - Exploring the Role of the Biological Clock ... June 16, 2022 - Fleshing Out a Fungal Toxins Role in Liver Cancer June 16, 2022 - Fleshing Out a Fungal Toxins Role in Liver ...
Additionally we analyzed the quality of human and fungal DNA and their level of age-dependent degradation, as well as the ... Quality of DNA in FFPE tissue samples was evaluated using fragments of the beta-globin gene for multiplex PCR, inhibition of ... The PCRs were applied to 27 FFPE specimens from 26 patients with proven invasive fungal disease (IFD), and one patient with ... tissue is challenging due to degradation of DNA and presence of PCR inhibitors in these samples. We analyzed FFPE samples of 26 ...
Could Trees Benefit from Fungal Transplants? A Bacterial Enzyme That Copies DNA Might Make More Mistakes in Zero Gravity, Study ... A Bacterial Enzyme That Copies DNA Might Make More Mistakes in Zero Gravity, Study Finds ...
The blue-green pigment(s) in P. italicum prevent DNA damage caused by 8-MOP most probably by screening the UV-A wavelengths ... Eight fungal genera were listed from a total of 355 fungal colonies: Aspergillus (4 sp.), Penicillium sp, Cladosporium sp, ... Airborne fungal spores can be easily removed by MERV filters (i.e. a MERV 13 filter) and the combination of UV and MERV filters ... Fungal colonization of air filters and insulation in a. multi-story office building: production of volatile organics. Curr. ...
Could Trees Benefit from Fungal Transplants? A Bacterial Enzyme That Copies DNA Might Make More Mistakes in Zero Gravity, Study ... A Bacterial Enzyme That Copies DNA Might Make More Mistakes in Zero Gravity, Study Finds ...
... were designed by targeting mycobiont-specific nucleotide sites in comparison with environmental fungal sequences, and assessed ... ribosomal DNA is one of the most commonly used loci for phylogenetic analysis of lichen-forming fungi, but their primer ... design mycobiont-specific mtSSU primers and highlights their utility with an example from the saxicolous lichen-forming fungal ...
A safe inexpensive method to isolate high quality plant and fungal DNA in an open laboratory environment - Texas A&M University ... A safe inexpensive method to isolate high quality plant and fungal DNA in an open laboratory environment Academic Article * ... A simple, economical and rapid method is presented to isolate high quality DNA from plant and fungal species. This method uses ... Further DNA purification is achieved using a low salt CTAB treatment. This SDS/CTAB protocol was used to isolate high quality ...
The protocol has been validated for thousands of DNA samples by generating high data quality in dense SNP arrays. DNA extracted ... herbarium leaf material and lyophilized fungal mycelium. We consistently obtained good yields of high purity DNA or RNA in all ... Scaled-up DNA extractions were also suitable for long read sequencing. Successful RNA quality control and good RNA-Seq data for ... We therefore investigated the utility of a pre-wash step using a buffered sorbitol solution, prior to DNA extraction using a ...
Fungal Genet Biol. 2020 Mar;136:103315. doi: 10.1016/j.fgb.2019.103315. Epub 2019 Dec 6. ... DNA-Binding Proteins / genetics * DNA-Binding Proteins / metabolism * Endo-1,4-beta Xylanases / genetics* ...
... studying yeast DNA and watching the movements of molecules. ... Sydnie extracted fungal DNA from her samples and then ran ... Biology and chemistry research: Fungal populations, DNA-protecting chromatin and plastic ingredients. This summer, three ... send the DNA to a company for genetic sequencing and can then match the results to DNA in a database to identify the fungal ... Condensed chromatin protects DNA, blocking most proteins from accessing it. Julianna and her faculty mentor, Dan Ginsburg, Ph.D ...
4. Multiple DNA binding domains mediate the function of the ERCC1-XPF protein in nucleotide excision repair.. Su Y; Orelli B; ... Fungal Genet Biol; 2023 Jan; 164():103752. PubMed ID: 36435348. [TBL] [Abstract]. ... 2. ERCC1 mutations impede DNA damage repair and cause liver and kidney dysfunction in patients.. Apelt K; White SM; Kim HS; Yeo ... ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2.. Sabatella M; Pines A; Slyskova J; Vermeulen W; Lans ...
... from both fungal cultures and blood. ... DNA & RNA Purification. DNA. Genomic DNA. QIAamp DNA Kits. ... poly A+ mRNA, DNA or protein. Genomic DNA, mitochondrial DNA. Genomic DNA, mitochondrial DNA,. bacterial DNA, parasite DNA, ... The QIAamp DNA Mini Kit yields DNA sized up to 50 kb (see figure " Purification of up to 50 kb genomic DNA"). DNA of this ... For QIAamp DNA Mini Kit (50) plus QIAamp DNA Accessory Set A, cat. no. 1048145, or QIAamp DNA Mini Kit (250) plus QIAamp DNA ...
Fungal Architectures and Logical Bacteria Karen Emslie. Commissioned by CACM Staff Can Crowdsourcing Rescue the Social ... Hacking Your Dna. By Newsweek March 17, 2014. Comments .fav_bar { float:left; border:1px solid #a7b1b5; margin-top:10px; margin ... Home/Opinion/Articles/Hacking Your Dna/Full Text ACM Opinion ...
  • Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and sequencing of specific DNA sequences and their comparison with sequences deposited in a DNA barcode database such as the ISHAM reference database, or the Barcode of Life Data System (BOLD). (wikipedia.org)
  • The interspecific variation, i.e., the variation between species, in the chosen DNA barcode gene should exceed the intraspecific (within-species) variation. (wikipedia.org)
  • Moreover, fungal species can comprise multiple strains that can vary in their morphology or in traits such as carbon- and nitrogen utilisation, which has often led to their description as different species, eventually producing long lists of synonyms. (wikipedia.org)
  • One group, led by researchers at the Weizmann Institute of Science in Israel and the University of California, San Diego (UCSD), reports detecting fungal DNA or cells-typically at low abundance-in 35 different cancer types, with fungal species composition differing among them. (the-scientist.com)
  • Across various sites, "several Candida species were enriched in tumor samples and tumor-associated Candida DNA was predictive of decreased survival," the authors write in their paper. (the-scientist.com)
  • Thus, having an efficient species-level identification system could significantly enhance our ability to treat fungal diseases and to monitor the spatial and temporal patterns of fungal distributions and migrations. (nih.gov)
  • DNA barcoding is a potent approach for rapid identification of fungal specimens, generating novel species hypothesis, and guiding biodiversity and ecological studies. (nih.gov)
  • In view of the increasing diversity of fungal pathogens in high risk patients, the differences in the antifungal spectrum of the available agents, and the looming threat of resistance, identification of the infecting isolate at the species level and information on drug resistance are important in order to provide state of the art patient care. (medscape.com)
  • Based on DNA sequence comparisons and other taxonomic characteristics, they were identified as more than four species of yeast and four species of mold. (iwaponline.com)
  • A simple, economical and rapid method is presented to isolate high quality DNA from plant and fungal species. (tamu.edu)
  • Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites. (pacb.com)
  • The method was successfully applied to a variety of tissues, including leaf, cambium and fruit of diverse plant species including annual crops, forest and fruit trees, herbarium leaf material and lyophilized fungal mycelium. (pacb.com)
  • We consistently obtained good yields of high purity DNA or RNA in all species tested. (pacb.com)
  • This broad project can be used to answer a variety of questions, Sydnie says, such as how different fungal species interact and whether temperature or location affect the rate of decomposition or the fungal communities that grow. (immaculata.edu)
  • The current study was designed to check the potential of keratinolytic fungal species for the conversion of chicken-feather waste into biofortified compost. (scielo.br)
  • For the purpose, three fungal species were isolated from soil samples. (scielo.br)
  • The degradation potential of these fungal species showed their ability to form compost that has agro-industrial importance. (scielo.br)
  • Its ribosomal DNA sequences of 18S and ITS have no similarity with any known fungal species. (scirp.org)
  • It involves using a short DNA sequence like a barcode exclusive to each species. (sciencedaily.com)
  • Experts estimate that more than 200 species have gone extinct after these fungal outbreaks. (mongabay.com)
  • To find evidence of the disease, Kolby searched for fungal DNA on skin swabs from 565 amphibians, representing nine different frog species that were exported from Madagascar directly to the U.S. He specifically chose ones that came from habitats that would be most hospitable to the fungus. (mongabay.com)
  • The present invention is based on a plurality of benefits from the extracts of mycelia of individual fungal species, and mixtures of species, to provide an armamentarium of defenses from multiple stressors in order to help bees survive a complex of symptoms collectively called colony collapse disorder (CCD). (justia.com)
  • Unification of the methodology, detailed descriptions of environmental factors, more reference sequences in public databases, and especially data on ecology and quantitative parameters of key fungal species would significantly improve the understanding of this issue. (sisef.it)
  • Here we use long-read sequencing to define the extent of HGT between these three fungal species. (biorxiv.org)
  • These horizontal transfer events are now in various processes of decay in each species due to the repeated insertion of new transposons and subsequent rounds of targeted mutation by a fungal genome defense mechanism known as repeat induced point-mutation. (biorxiv.org)
  • Twice weekly screening in blood of high risk patients [ 24 ] and specific detection of fungal pathogens in tissue specimens [ 25 ] have emerged as the most feasible clinical applications to date. (medscape.com)
  • The present study aims to rapidly identify Periplaneta americana fungal pathogens from sewer systems of public hospitals in Esfahan using the polymerase chain reaction (PCR) technique. (iwaponline.com)
  • Our results show that the use of a combination of morphological, molecular techniques, and phylogenetic analysis can lead to the identification of pathogenic fungal agents in American cockroaches and also knowledge of fungal pathogens-arthropod host relationships. (iwaponline.com)
  • Periplaneta americana acts as a potential mechanical vector of medically important fungal pathogens from inside sewer systems to hospitals and conversely. (iwaponline.com)
  • As reported in the journal Plant Disease,  a recent collaboration between National Library of Medicine’s NCBI and the U.S. Department of Agriculture’s Animal and Plant Health Inspection Service (APHIS) analyzed public sequence records for the fungal genus Colletotrichum, an important group of fungal plant pathogens that are a significant threat  to food production. (nih.gov)
  • The aim of the present study was the evaluation of two diagnostic panfungal PCR detection systems based on ITS amplification followed by sequencing for the identification of fungal pathogens in 26 culturally or histologically proven cases of IFD and one additional PCR positive case in FFPE tissue specimens. (biomedcentral.com)
  • Is the Subject Area "Plant fungal pathogens" applicable to this article? (plos.org)
  • In 1991 he started his own research group at the RWTH Aachen, Germany, with a focus on plant disease resistance mechanisms to fungal pathogens. (mpg.de)
  • ToxA has been horizontally transferred between three fungal wheat pathogens ( Parastagonospora nodorum, Pyrenophora tritici-repentis and Bipolaris sorokiniana ) as part of a conserved ∼14kb element, which contains coding and non-coding regions. (biorxiv.org)
  • The beta-D glucan assay has been studied in adult patients with fungal infections. (medscape.com)
  • Cite this: Invasive Fungal Infections in Children - Medscape - Aug 01, 2009. (medscape.com)
  • All relevant materials for patients and clinicians concerning the multistate outbreak of fungal meningitis and other infections are located on this page. (cdc.gov)
  • This relapse incidence is lower than the prevalence of relapse observed for other invasive fungal infections. (cdc.gov)
  • Prior to studies of and broad institution of antifungal prophylaxis in high-risk preterm infants, the incidence of fungal infections had been rising in infants born at less than 1000 g, with the ensuing resuscitation and survival of more and more infants. (medscape.com)
  • For these reasons, fungal infections are often difficult to eradicate in the preterm infant and, in cases of candidemia, central venous catheter removal is critical for clearance and survival. (medscape.com)
  • Although these immunocompromised infants are at increased risk during most of their hospital stay, they are at the highest risk of acquiring invasive fungal infections during the first weeks of life, when the most invasive therapies are performed and remain in place. (medscape.com)
  • The pathogenesis of fungal infections in preterm infants involves adherence, colonization, and dissemination (as shown in the image below). (medscape.com)
  • Pathogenesis and invasive fungal infections in very low birth weight infants. (medscape.com)
  • fungal infections are rare ( 5 - 7 ). (cdc.gov)
  • On March 5, 2012, the Healthcare Associated Infections Program of the California Department of Public Health was alerted to a cluster of 9 cases of fungal endophthalmitis, all among patients at the same Los Angeles County ambulatory surgical center who had undergone pars plana vitrectomies during October-December 2011. (cdc.gov)
  • Fungal skin infections affect an estimated 29 million people nationwide. (nih.gov)
  • But fungi can be slow and difficult to grow in laboratories, making fungal infections hard to identify and treat. (nih.gov)
  • By gaining a more complete awareness of the fungal and bacterial ecosystems, we can better address associated skin diseases, including fungal infections, which can be related to cancer treatments. (nih.gov)
  • IL17RC is a protein that promotes immune responses to infections, such as fungal attacks. (nih.gov)
  • Overview of Fungal Infections Fungi are eukaryotic organisms that exist as yeast, molds, or both forms. (msdmanuals.com)
  • Furthermore, the importance of the accurate and timely diagnosis of fungal infections in defeating AMR has been starkly absent from policy discussions ( 4 ). (cdc.gov)
  • We provide 4 examples of specific clinical situations that require greater application of existing fungal diagnostics and improved overall fungal diagnostic capability and are in line with the 95-95 by 2025 Roadmap from the Global Action Fund for Fungal Infections ( 6 ). (cdc.gov)
  • Toe webs, with about 60 types, and toenails, with 40, had the next highest levels of fungal diversity. (nih.gov)
  • The bottom line is your feet are teeming with fungal diversity, so wear your flip flops in locker rooms if you don't want to mix your foot fungi with someone else's fungi. (nih.gov)
  • Fungal and bacterial diversity was assessed with terminal restriction fragment length polymorphism (tRFLP) and defined by Simpson's Diversity Index. (nih.gov)
  • Adjusted logistic regression analyses revealed a significantly reduced risk of developing sensitization to aero-allergens at 6 years and ever wheezing until the age of 10 years for exposure to higher fungal diversity [adjusted odds ratio (aOR) = 0.26 (95% CI: 0.10, 0.70), and 0.42 (95% CI: 0.18, 0.96), respectively]. (nih.gov)
  • Higher early exposure to fungal diversity might help to prevent a child from developing sensitization to aero-allergens in early childhood, but the reasons for attenuated effects in later childhood require further prospective studies. (nih.gov)
  • Specific aim 1: Assess HHCWs' exposure to bioaerosols by DNA quantification with qPCR and by the determination of the diversity and the abundance through metagenomic with NGS in the air and surface samples collected in the visited homes to be compared to other occupational studies. (cdc.gov)
  • This review provides an extensive understanding of the diversity and ecological dynamics of orchid-fungal association. (frontiersin.org)
  • We provide a comprehensive overview, systematically describing six fields of research on orchid-fungal diversity: the research methods of orchid-fungal interactions, the primer selection in high-throughput sequencing, the fungal diversity and specificity in orchids, the difference and adaptability of OMF in different habitats, the comparison of OMF in orchid roots and soil, and the spatiotemporal variation patterns of OMF. (frontiersin.org)
  • DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica. (bas.ac.uk)
  • Belowground fungal diversity is very important in terrestrial ecosystems, however, the effect of land use change on soil fungal community is poorly understood. (sisef.it)
  • To monitor the effect of land use change, different fungal parameters such as richness, diversity, community composition, root colonization by arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) fungi, spore density, ergosterol, and phospholipid fatty acid (PLFA) content and AM fungal glomalin related soil protein (GRSP) were studied. (sisef.it)
  • He will also touch on Ohio's fungal diversity. (dailyadvocate.com)
  • In this attempt, DNA barcoding relies on universal genes that are ideally present in all fungi with the same degree of sequence variation. (wikipedia.org)
  • Fungal DNA barcoding can help to identify and associate anamorphic and teleomorphic stages of fungi, and through that to reduce the confusing multitude of fungus names. (wikipedia.org)
  • The success of identification of fungi by means of DNA barcode sequences stands and falls with the quantitative (completeness) and qualitative (level of identification) aspect of the reference database. (wikipedia.org)
  • 2012) proposed the ITS region as primary DNA barcode region for the genetic identification of fungi. (wikipedia.org)
  • This SDS/CTAB protocol was used to isolate high quality genomic DNA subject to restriction endonuclease digestion and AFLP analysis from both plant and fungi with minimum cost and health concerns. (tamu.edu)
  • This summer, three undergraduate students are getting their hands dirty with scientific research-growing fungi, studying yeast DNA and watching the movements of molecules. (immaculata.edu)
  • They focused on a fragment of DNA shared by all fungi - the intervening internal transcribed spacer 1 (ITS1) of ribosomal RNA - that could be used to classify fungi at the genus level with greater than 97% accuracy. (nih.gov)
  • Fungal metagenomics is building knowledge on fungi within different environments (the environmental mycobiome), fungi on and within the human body (the human mycobiome), and their association with disease. (springer.com)
  • We aimed to investigate the early fungal and bacterial microbiome in house dust with allergic sensitization and wheezing later in childhood. (nih.gov)
  • Individual dust samples from 189 homes of the LISAplus birth cohort study were collected shortly after birth from living room floors and profiled for fungal and bacterial microbiome. (nih.gov)
  • The research team tracked fungal and bacterial communities in fog delivered to two fog-dominated sites: Southport Island, Maine in the United States and the Namib Desert in Namibia. (eurasiareview.com)
  • They also show significant to good antimicrobial activity against six bacterial and five fungal strains. (unboundmedicine.com)
  • Voucher specimens should be clearly labelled as such, including a permanent voucher identifier that unambiguously connects the specimen with the DNA barcode data derived from it. (wikipedia.org)
  • The PCRs were applied to 27 FFPE specimens from 26 patients with proven invasive fungal disease (IFD), and one patient with culture and histologically negative but PCR-positive fungal infection collected at our institution from 2003 to 2010. (biomedcentral.com)
  • Panfungal diagnostic PCR assays applied on FFPE specimens provide accurate identification of molds in highly degraded tissue samples and correct identification in samples stored up to 7 years despite sensitivity limitations, mainly caused by partial PCR inhibition and DNA degradation by formalin. (biomedcentral.com)
  • Detection of fungal DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is challenging due to degradation of DNA and presence of PCR inhibitors in these samples. (biomedcentral.com)
  • Here, it was found that the green manures of Vicia villosa (leguminous) and Brassica juncea (non-leguminous) have different fungal structures, despite the soil originally being the same. (mdpi.com)
  • The following update provides information on clinical follow-up of patients affected in the unprecedented multistate invasive fungal infection outbreak associated with contaminated methylprednisolone injections in late 2012 and 2013. (cdc.gov)
  • In March 2012, several cases of probable and laboratory-confirmed fungal endophthalmitis occurring after invasive ocular procedures were reported nationwide. (cdc.gov)
  • Invasive fungal disease (IFD), especially in case of delayed initiation of appropriate antifungal treatment, is associated with severe morbidity and high mortality in immunocompromised, critically-ill patients [ 1 ]. (biomedcentral.com)
  • Extensive loss of cell-cycle and DNA repair genes in an ancient lineage of bipolar budding yeasts. (nih.gov)
  • Similarly, both lineages lost genes involved in DNA repair pathways, including the DNA glycosylase gene 3-MethylAdenine DNA Glycosylase 1 (MAG1), which is part of the base-excision repair pathway, and the DNA photolyase gene PHotoreactivation Repair deficient 1 (PHR1), which is involved in pyrimidine dimer repair. (nih.gov)
  • The study identified decreased levels of DNA methylation, a chemical reaction that switches off genes, on the interleukin-17 receptor C gene (IL17RC). (nih.gov)
  • Among the 231 genes, the investigators found that DNA methylation was absent in a region of the IL17RC gene in twins with AMD. (nih.gov)
  • T. J. White, T. Bruns, S. Lee and J. W. Taylor, "Amplification and Direct Sequencing of Fungal Ribosomal RNA genes for Phylogenetics," In: M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, Eds. (scirp.org)
  • the others had a clinical diagnosis of fungal endophthalmitis. (cdc.gov)
  • [ 17 ] While cross-reactions with other fungal organisms are rare, false-positive results can be caused by contaminating GM in ß-lactam-antibiotics, dietary GM in pasta, cereals and formula milk, and cross reactivity with lipoteichoic acids of Bifidobacteria of the infantile gut microflora. (medscape.com)
  • Significant time-dependent degradation of human DNA in FFPE sample extracts was detected based on partial beta-globin gene amplification which was not in correlation to successful panfungal PCR identification of fungal organisms. (biomedcentral.com)
  • The two fungal internal transcribed spacer regions 1 (ITS1) and 2 (ITS2) are highly variable and can be amplified by PCR using broad-range panfungal primers detecting potentially all fungal organisms [ 6 ]. (biomedcentral.com)
  • She then swabbed plates that indicated growing organisms and incubated the plates to see what fungal populations would develop. (immaculata.edu)
  • Cell-cycle checkpoints and DNA repair processes protect organisms from potentially lethal mutational damage. (nih.gov)
  • Condensed chromatin protects DNA, blocking most proteins from accessing it. (immaculata.edu)
  • The lack of DNA methylation led to increased gene activity and, in turn, increased levels of IL17RC proteins in patients with AMD. (nih.gov)
  • Yutin and Koonin, 2012 ) constitute an apparently monophyletic group of eukaryotic viruses with a large double-stranded DNA (dsDNA) genome ranging from 100 kb up to 1.26 Mb. (nature.com)
  • DNA sequence alignment is a hard problem that seeks to arrange two or more genome sequences in order to identify regions of similarity. (elifesciences.org)
  • A total of 55 P. americana cockroaches were randomly collected by direct trapping from sewer systems of seven hospitals and screened for fungal infectious agents using standard morphological methods and the PCR sequencing. (iwaponline.com)
  • Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications. (pacb.com)
  • Scaled-up DNA extractions were also suitable for long read sequencing. (pacb.com)
  • She will send the DNA to a company for genetic sequencing and can then match the results to DNA in a database to identify the fungal populations. (immaculata.edu)
  • In this program, Canan will focus on mushroom identification as well as offering an introduction to DNA sequencing. (dailyadvocate.com)
  • When the identity of a certain taxon (or a genetic sequence in the case of DNA barcoding) is in doubt, the original specimen can be re-examined to review and ideally solve the issue. (wikipedia.org)
  • Like the flanking 18S and 28S subunits, the 5.8S subunit contains a highly conserved DNA sequence, as they code for structural parts of the ribosome, which is a key component in intracellular protein synthesis. (wikipedia.org)
  • The solution structure of a complex between the DNA binding domain of a fungal GATA factor and a 13 base-pair oligonucleotide containing its physiologically relevant CGATAG target sequence has been determined by multidimensional nuclear magnetic resonance spectroscopy. (nih.gov)
  • Subsequently, we developed and released Fraxinus, a Facebook-based game, to allow non-specialists to improve genetic variant predictions from DNA sequence data arising from the OADB project ( MacLean, 2013 ). (elifesciences.org)
  • Changes observable on MRI consistent with a fungal infection have proven valuable in the identification of cases, especially in patients with a history of significant baseline pain. (cdc.gov)
  • All of these factors place them at high risk for fungal infection. (medscape.com)
  • Fungal endophthalmitis is a rare but serious infection. (cdc.gov)
  • Within the context of stewardship programs, inadequate attention has been paid to fungal infection as the cause of antibacterial treatment failure. (cdc.gov)
  • Accurate diagnosis or exclusion of fungal infection will have a substantial effect on antimicrobial drug usage and on our ability to limit AMR to bacteria. (cdc.gov)
  • Factors such as the composting method and the ingredients that go into the compost cause different types of fungal communities to grow. (immaculata.edu)
  • Fungal communities on the core body were quite stable over time, with little change when tested up to 3 months apart. (nih.gov)
  • The fungal communities on the human body are complex and site-specific," says co-senior author Dr. Heidi Kong of NCI. (nih.gov)
  • Soil fungal communities across land use types. (sisef.it)
  • In general, land use change is a function of changes in vegetation structure, soil properties, and anthropogenic activities which are known to influence the soil fungal communities ( [68] ). (sisef.it)
  • All communities should have access to nonculture fungal diagnostics, which can substantially benefit clinical outcome, antimicrobial stewardship, and control of antimicrobial resistance. (cdc.gov)
  • We describe 2 concurrent multistate outbreaks of fungal endophthalmitis associated with intraocular use of contaminated products labeled as sterile from a single compounding pharmacy. (cdc.gov)
  • however, several studies have shown that visual acuity is not a useful factor in assessing the presence of endogenous fungal endophthalmitis in patients who are at risk. (medscape.com)
  • By generating more than 5 million ITS1 sequences from the samples, the team was able to identify more than 80 genus-level fungal types living on human skin. (nih.gov)
  • Identical twins have the same genetic makeup while fraternal twins share about half of their DNA. (nih.gov)
  • The genetic barcode, also known as DNA barcoding, is a very useful tool. (sciencedaily.com)
  • Major research areas are the innate immune system of plants, mechanisms of fungal pathogenesis, and the plant microbiota. (mpg.de)
  • Quality of DNA in FFPE tissue samples was evaluated using fragments of the beta-globin gene for multiplex PCR, inhibition of PCR amplification was evaluated by spiking of C. krusei DNA to each PCR premix. (biomedcentral.com)
  • DNA of this length denatures completely and has the highest amplification efficiency. (qiagen.com)
  • The QIAamp DNA Mini QIAcube Kit yields DNA sized up to 50 kb: DNA of this length denatures completely and has the highest amplification efficiency. (qiagen.com)
  • These DNA repair pathways are highly conserved between yeast, DT40, and humans. (nih.gov)
  • 10. The XPA-binding domain of ERCC1 is required for nucleotide excision repair but not other DNA repair pathways. (nih.gov)
  • We therefore investigated the utility of a pre-wash step using a buffered sorbitol solution, prior to DNA extraction using a high salt CTAB extraction protocol, in a high throughput or miniprep setting. (pacb.com)
  • Commercial DNA extraction kits are convenient and usually safe, but their availability to certain developing countries and high cost can be limiting, especially when handing a large number of samples and considering experiments with limited financial resources. (tamu.edu)
  • The most commonly used plant DNA isolation methods use toxic and hazardous chemicals (phenol, chloroform), which require special equipment to minimize exposure and may limit their use in certain environments. (tamu.edu)
  • T. Matsubara, M. Yoneda and T. Ishii, "Fungal Isolate "KMI" Is a New Type of Orchid Mycorrhizal Fungus," American Journal of Plant Sciences , Vol. 3 No. 8, 2012, pp. 1121-1126. (scirp.org)
  • He worked from 1989 to 1990 as postdoctoral fellow on the development of DNA marker technologies in plant genomes. (mpg.de)
  • Combining the results therefore provided a more comprehensive analysis across all fungal taxonomic ranks, identifying statistically significant differences in the fungal composition of the five soil types. (springer.com)
  • The region is flanked by the DNA sequences for the ribosomal small subunit (SSU) or 18S subunit at the 5' end, and by the large subunit (LSU) or 28S subunit at the 3' end. (wikipedia.org)
  • The AREA DNA binding domain, from Aspergillus nidulans, possesses a single Cys2-Cys2 zinc finger module and a basic C-terminal tail, which recognize the CGATAG element via an extensive network of hydrophobic interactions with the bases in the major groove and numerous non-specific contacts along the sugar-phosphate backbone. (nih.gov)
  • Each holds the DNA blueprint for a fungus, and it can faithfully preserve that DNA for astonishing periods of time. (cosmosmagazine.com)
  • In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. (qiagen.com)
  • Land use change mostly showed significant shifts in fungal community composition. (sisef.it)
  • All these 18 types of land use change influenced fungal community composition, however, the effects on quantitative parameters were mostly inconsistent. (sisef.it)
  • DNA vaccines against Zika virus speed into clinical trials. (cdc.gov)
  • Additionally we analyzed the quality of human and fungal DNA and their level of age-dependent degradation, as well as the existence of PCR inhibition in these tissue samples. (biomedcentral.com)
  • The protocol has been validated for thousands of DNA samples by generating high data quality in dense SNP arrays. (pacb.com)
  • Sydnie extracted fungal DNA from her samples and then ran polymerase chain reactions (PCR), which amplify a small section of DNA into many copies. (immaculata.edu)
  • Julianna then exposed both samples to two different DNA-damaging carcinogens, placed the cultures on a growing medium, and counted the colonies of yeast that grew. (immaculata.edu)
  • DNA purification from 1-12 samples can be automated on the QIAcube Connect using the dedicated QIAamp DNA Mini QIAcube Kit. (qiagen.com)
  • The QIAamp DNA Mini QIAcube Kit simplifies DNA isolation from tissue samples with fast spin-column procedures. (qiagen.com)
  • The title compounds have been screened for their biological activities including, antibacterial, antifungal, antioxidant, cytotoxic, enzymatic activities as well as interaction with SS-DNA which showed remarkable activities in each area of research. (unboundmedicine.com)
  • Lack of routine diagnostic testing for fungal diseases exacerbates the problem of antimicrobial drug empiricism, both antibiotic and antifungal. (cdc.gov)
  • Similar to DNA barcoding research on plants and animals, significant progress has been achieved over the last few years in terms of both the questions being addressed and the foundations being laid for future research endeavors. (nih.gov)
  • Purified DNA may also be used in short-tandem repeat (STR) genotyping, single-nucleotide polymorphism (SNP) genotyping or pharmacogenomic research. (qiagen.com)
  • DNA purified using the QIAamp DNA Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, Southern blotting, SNP and STR genotyping and pharmacogenomic research. (qiagen.com)
  • Nucleo-cytoplasmic large DNA viruses (NCLDVs) constitute a group of eukaryotic viruses that can have crucial ecological roles in the sea by accelerating the turnover of their unicellular hosts or by causing diseases in animals. (nature.com)
  • Changes in DNA methylation have been implicated in cancer, lupus, multiple sclerosis, and many other diseases. (nih.gov)
  • QIAamp DNA Kits are intended for molecular biology applications. (qiagen.com)
  • This study strongly implicates epigenetic DNA methylation as another crucial biological pathway for understanding the molecular basis of AMD," according to Nussenblatt. (nih.gov)
  • Assignment of fungal taxa into ecological guilds revealed those differences of biological relevance to public health. (springer.com)
  • This review emphasizes the need for more information on the four main ecological processes: dispersal, selection, ecological drift, and diversification, as well as their interactions, in the study of orchid-fungal interactions and OMF community structure. (frontiersin.org)
  • Sample analysis: Real-time polymerase chain reaction (qPCR) with universal primer to quantify DNA copy of microbial agents. (cdc.gov)
  • Ash dieback is a disease caused by the fungal pathogen Hymenoscyphus fraxineus , and it has devastated populations of ash trees ( Fraxinus excelsior ) across Europe in recent years. (elifesciences.org)
  • In this study, we use DNA sequences from four closely related clades of Saccharomyces paradoxus and Saccharomyces cerevisiae to identify and polarize new mutations and estimate their fitness effects. (imperial.ac.uk)
  • 4. Multiple DNA binding domains mediate the function of the ERCC1-XPF protein in nucleotide excision repair. (nih.gov)
  • Another crucial prerequisite for DNA barcoding is the ability to unambiguously trace the provenance of DNA barcode data back to the originally sampled specimen, the so-called voucher specimen. (wikipedia.org)
  • Our findings are epigenetic in nature, meaning that the underlying DNA is normal but gene expression has been modified, likely by environmental factors, in an adverse way," said Dr. Robert Nussenblatt, chief of the National Eye Institute (NEI) Laboratory of Immunology. (nih.gov)
  • F ungal DNA is present in various types of cancer, according to two studies published yesterday (September 29) in Cell . (the-scientist.com)
  • The fungal levels following UV operation were significantly lower than the levels in control AHUs. (researchgate.net)
  • 2. ERCC1 mutations impede DNA damage repair and cause liver and kidney dysfunction in patients. (nih.gov)
  • 5. Mislocalization of XPF-ERCC1 nuclease contributes to reduced DNA repair in XP-F patients. (nih.gov)
  • An appealing alternative for culture-independent identification of the fungal agent might be panfungal PCR performed retrospectively from FFPE tissue. (biomedcentral.com)
  • Construction of near-chromosomal level assemblies enabled identification of terminal inverted repeats on either end of the 14kb region, typical of a Type II DNA transposon. (biorxiv.org)
  • Performances of two different panfungal PCRs to detect mould DNA in formalin-fixed paraffin-embedded tissue: what are the limiting factors? (biomedcentral.com)