Centromere protein b. Medical search

A DNA-binding protein that interacts with a 17-base pair sequence known as the CENP-B box motif. The protein is localized constitutively to the CENTROMERE and plays an important role in its maintenance.

The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.

Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.

Endogenous tissue constituents that have the ability to interact with AUTOANTIBODIES and cause an immune response.

Large multiprotein complexes that bind the centromeres of the chromosomes to the microtubules of the mitotic spindle during metaphase in the cell cycle.

A pulmonary surfactant associated-protein that plays an essential role in alveolar stability by lowering the surface tension at the air-liquid interface. Inherited deficiency of pulmonary surfactant-associated protein B is one cause of RESPIRATORY DISTRESS SYNDROME, NEWBORN.

The orderly segregation of CHROMOSOMES during MEIOSIS or MITOSIS.

A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.

An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. It mediates proper CHROMOSOME SEGREGATION and contractile ring function during CYTOKINESIS.

A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. They are involved in many aspects of cell division, including centrosome duplication, SPINDLE APPARATUS formation, chromosome alignment, attachment to the spindle, checkpoint activation, and CYTOKINESIS.

Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.

In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

A subtype of bacterial transferrin-binding protein found in bacteria. It forms a cell surface receptor complex with TRANSFERRIN-BINDING PROTEIN A.

Extreme reduction of chromosome-specific alpha-satellite array is unusually common in human chromosome 21. (1/99)

Human centromeres contain large arrays of alpha-satellite DNA that are thought to provide centromere function. The arrays show size and sequence variation, but the extent to which extremely low levels of this DNA can occur on normal centromeres is unclear. Using a set of chromosome-specific alpha-satellite probes for each of the human chromosomes, we performed interphase fluorescence in situ hybridization (FISH) in a population-screening study. Our results demonstrate that extreme reduction of chromosome-specific alpha satellite is unusually common in chromosome 21 (screened with the alphaRI probe), with a prevalence of 3.70%, compared to < or =0.12% for each of chromosomes 13 and 17, and 0% for the other chromosomes. No analphoid centromere was identified in >17,000 morphologically normal chromosomes studied. All of the low-alphoid centromeres are fully functional as indicated by their mitotic stability and binding to centromere proteins CENP-B, CENP-C, and CENP-E. Sensitive metaphase FISH analysis of the low-alphoid chromosome 21 centromeres established the presence of residual alphaRI as well as other non-alphaRI alpha-satellite DNA suggesting that centromere function may be provided by (1) the residual alphaRI DNA, (2) other non-alphaRI alpha-satellite sequences, (3) a combination of 1 and 2, or (4) an activated neocentromere DNA. The low-alphoid centromeres, in particular those of chromosome 21, should provide unique opportunities for the study of the evolution and the minimal DNA requirement of the human centromere. (+info)

Uterine dysfunction and genetic modifiers in centromere protein B-deficient mice. (2/99)

Centromere protein B (CENP-B) binds constitutively to mammalian centromere repeat DNA and is highly conserved between humans and mouse. Cenpb null mice appear normal but have lower body and testis weights. We demonstrate here that testis-weight reduction is seen in male null mice generated on three different genetic backgrounds (denoted R1, W9.5, and C57), whereas body-weight reduction is dependent on the genetic background as well as the gender of the animals. In addition, Cenpb null females show 31%, 33%, and 44% reduced uterine weights on the R1, W9.5, and C57 backgrounds, respectively. Production of "revertant" mice lacking the targeted frameshift mutation but not the other components of the targeting construct corrected these differences, indicating that the observed phenotype is attributable to Cenpb gene disruption rather than a neighbouring gene effect induced by the targeting construct. The R1 and W9.5 Cenpb null females are reproductively competent but show age-dependent reproductive deterioration leading to a complete breakdown at or before 9 months of age. Reproductive dysfunction is much more severe in the C57 background as Cenpb null females are totally incompetent or are capable of producing no more than one litter. These results implicate a further genetic modifier effect on female reproductive performance. Histology of the uterus reveals normal myometrium and endometrium but grossly disrupted luminal and glandular epithelium. Tissue in situ hybridization demonstrates high Cenpb expression in the uterine epithelium of wild-type animals. This study details the first significant phenotype of Cenpb gene disruption and suggests an important role of Cenpb in uterine morphogenesis and function that may have direct implications for human reproductive pathology. (+info)

Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. (3/99)

Centromere protein A (Cenpa for mouse, CENP-A for other species) is a histone H3-like protein that is thought to be involved in the nucleosomal packaging of centromeric DNA. Using gene targeting, we have disrupted the mouse Cenpa gene and demonstrated that the gene is essential. Heterozygous mice are healthy and fertile whereas null mutants fail to survive beyond 6.5 days postconception. Affected embryos show severe mitotic problems, including micronuclei and macronuclei formation, nuclear bridging and blebbing, and chromatin fragmentation and hypercondensation. Immunofluorescence analysis of interphase cells at day 5.5 reveals complete Cenpa depletion, diffuse Cenpb foci, absence of discrete Cenpc signal on centromeres, and dispersion of Cenpb and Cenpc throughout the nucleus. These results suggest that Cenpa is essential for kinetochore targeting of Cenpc and plays an early role in organizing centromeric chromatin at interphase. The evidence is consistent with the proposal of a critical epigenetic function for CENP-A in marking a chromosomal region for centromere formation. (+info)

Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation. (4/99)

Two functionally important DNA sequence elements in centromeres of the fission yeast Schizosaccharomyces pombe are the centromeric central core and the K-type repeat. Both of these DNA elements show internal functional redundancy that is not correlated with a conserved DNA sequence. Specific, but degenerate, sequences in these elements are bound in vitro by the S. pombe DNA-binding proteins Abp1p (also called Cbp1p) and Cbhp, which are related to the mammalian centromere DNA-binding protein CENP-B. In this study, we determined that Abp1p binds to at least one of its target sequences within S. pombe centromere II central core (cc2) DNA with an affinity (K(s) = 7 x 10(9) M(-1)) higher than those of other known centromere DNA-binding proteins for their cognate targets. In vivo, epitope-tagged Cbhp associated with centromeric K repeat chromatin, as well as with noncentromeric regions. Like abp1(+)/cbp1(+), we found that cbh(+) is not essential in fission yeast, but a strain carrying deletions of both genes (Deltaabp1 Deltacbh) is extremely compromised in growth rate and morphology and missegregates chromosomes at very high frequency. The synergism between the two null mutations suggests that these proteins perform redundant functions in S. pombe chromosome segregation. In vitro assays with cell extracts with these proteins depleted allowed the specific assignments of several binding sites for them within cc2 and the K-type repeat. Redundancy observed at the centromere DNA level appears to be reflected at the protein level, as no single member of the CENP-B-related protein family is essential for proper chromosome segregation in fission yeast. The relevance of these findings to mammalian centromeres is discussed. (+info)

Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype. (5/99)

BACKGROUND: Survivin is a mammalian protein that carries a motif typical of the inhibitor of apoptosis (IAP)proteins, first identified in baculoviruses. Although baculoviral IAP proteins regulate cell death, the yeast Survivin homolog Bir1 is involved in cell division. To determine the function of Survivin in mammals, we analyzed the pattern of localization of Survivin protein during the cell cycle, and deleted its gene by homologous recombination in mice. RESULTS: In human cells, Survivin appeared first on centromeres bound to a novel para-polar axis during prophase/metaphase, relocated to the spindle midzone during anaphase/telophase, and disappeared at the end of telophase. In the mouse, Survivin was required for mitosis during development. Null embryos showed disrupted microtubule formation, became polyploid, and failed to survive beyond 4.5days post coitum. This phenotype, and the cell-cycle localization of Survivin, resembled closely those of INCENP. Because the yeast homolog of INCENP, Sli15, regulates the Aurora kinase homolog Ipl1p, and the yeast Survivin homolog Bir1 binds to Ndc10p, a substrate of Ipl1p, yeast Survivin, INCENP and Aurora homologs function in concert during cell division. CONCLUSIONS: In vertebrates, Survivin and INCENP have related roles in mitosis, coordinating events such as microtubule organization, cleavage-furrow formation and cytokinesis. Like their yeast homologs Bir1 and Sli15, they may also act together with the Aurora kinase. (+info)

Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. (6/99)

Three human centromere proteins, CENP-A, CENP-B and CENP-C, are a set of autoantigens specifically recognized by anticentromere antibodies often produced by patients with scleroderma. Microscopic observation has indicated that CENP-A and CENP-C localize to the inner plate of metaphase kinetochore, while CENP-B localizes to the centromere heterochromatin beneath the kinetochore. The antigenic structure, called "prekinetochore", is also present in interphase nuclei, but little is known about its molecular organization and the relative position of these antigens. Here, to visualize prekinetochore in living cells, we first obtained a stable human cell line, MDA-AF8-A2, in which human CENP-A is exogenously expressed as a fusion to a green fluorescent protein of Aequorea victoria. Simultaneous staining with anti-CENP-B and anti-CENP-C antibodies showed that the recombinant CENP-A colocalized with the endogenous CENP-C and constituted small discrete dots attaching to larger amorphous mass of CENP-B heterochromatin. When the cell growth was arrested in G1/ S phase with hydroxyurea, CENP-B heterochromatin was sometimes highly extended, while the relative location between GFP-fused CENP-A and the endogenous CENP-C was not affected. These results indicated that the fluorescent CENP-A faithfully localizes to the centromere/kinetochore throughout the cell cycle. We then obtained several mammalian cell lines where the same GFP-fused human CENP-A construct was stably expressed and their centromere/kinetochore is fluorescent throughout the cell cycle. These cell lines will further be used for visualizing the prekinetochore locus in interphase nuclei as well as analyzing kinetochore dynamics in the living cells. (+info)

Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B. (7/99)

Several members of protein families that are conserved in higher eukaryotes are known to play a role in centromere function in the fission yeast Schizosaccharomyces pombe, including two homologs of the mammalian centromere protein CENP-B, Abp1p and Cbh1p. Here we characterize a third S. pombe CENP-B homolog, Cbh2p (CENP-B homolog 2). cbh2Delta strains exhibited a modest elevation in minichromosome loss, similar to cbh1Delta or abp1Delta strains. cbh2Delta cbh1Delta strains showed little difference in growth or minichromosome loss rate when compared to single deletion strains. In contrast, cbh2Delta abp1Delta strains displayed dramatic morphological and chromosome segregation defects, as well as enhancement of the slow-growth phenotype of abp1Delta strains, indicating partial functional redundancy between these proteins. Both cbh2Delta abp1Delta and cbh1Delta abp1Delta strains also showed strongly enhanced sensitivity to a microtubule-destabilizing drug, consistent with a mitotic function for these proteins. Cbh2p was localized to the central core and core-associated repeat regions of centromeric heterochromatin, but not at several other centromeric and arm locations tested. Thus, like its mammalian counterpart, Cbh2p appeared to be localized exclusively to a portion of centromeric heterochromatin. In contrast, Abp1p was detected in both centromeric heterochromatin and in chromatin at two of three replication origins tested. Cbh2p and Abp1p homodimerized in the budding yeast two-hybrid assay, but did not interact with each other. These results suggest that indirect cooperation between different CENP-B-like DNA binding proteins with partially overlapping chromatin distributions helps to establish a functional centromere. (+info)

Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A. (8/99)

The mechanisms that specify precisely where mammalian kinetochores form within arrays of centromeric heterochromatin remain largely unknown. Localization of CENP-A exclusively beneath kinetochore plates suggests that this distinctive histone might direct kinetochore formation by altering the structure of heterochromatin within a sub-region of the centromere. To test this hypothesis, we experimentally mistargeted CENP-A to non-centromeric regions of chromatin and determined whether other centromere-kinetochore components were recruited. CENP-A-containing non-centromeric chromatin assembles a subset of centromere-kinetochore components, including CENP-C, hSMC1, and HZwint-1 by a mechanism that requires the unique CENP-A N-terminal tail. The sequence-specific DNA-binding protein CENP-B and the microtubule-associated proteins CENP-E and HZW10 were not recruited, and neocentromeric activity was not detected. Experimental mistargeting of CENP-A to inactive centromeres or to acentric double-minute chromosomes was also not sufficient to assemble complete kinetochore activity. The recruitment of centromere-kinetochore proteins to chromatin appears to be a unique function of CENP-A, as the mistargeting of other components was not sufficient for assembly of the same complex. Our results indicate at least two distinct steps in kinetochore assembly: (1) precise targeting of CENP-A, which is sufficient to assemble components of a centromere-prekinetochore scaffold; and (2) targeting of kinetochore microtubule-associated proteins by an additional mechanism present only at active centromeres. (+info)

Centromere Protein B (CENP-B) is a protein that plays a crucial role in the organization and function of centromeres, which are specialized regions of chromosomes where the spindle fibers attach during cell division. CENP-B is one of the proteins that make up the constitutive centromere-associated network (CCAN), which is a complex of proteins that forms the foundation of the kinetochore, the structure that connects the chromosome to the spindle fibers.

CENP-B has a unique ability to recognize and bind to specific DNA sequences within the centromere region called CENP-B boxes. This binding helps to establish and maintain the structural integrity of the centromere, ensuring that it functions correctly during cell division. Mutations in the CENP-B gene can lead to chromosomal instability and may contribute to the development of certain genetic disorders.

It's worth noting that while CENP-B is an important protein involved in centromere function, it is not present in all centromeres, and its absence does not necessarily mean that a centromere will be nonfunctional. Other proteins can compensate for the lack of CENP-B and help maintain centromere function.

A centromere is a specialized region found on chromosomes that plays a crucial role in the separation of replicated chromosomes during cell division. It is the point where the sister chromatids (the two copies of a chromosome formed during DNA replication) are joined together. The centromere contains highly repeated DNA sequences and proteins that form a complex structure known as the kinetochore, which serves as an attachment site for microtubules of the mitotic spindle during cell division.

During mitosis or meiosis, the kinetochore facilitates the movement of chromosomes by interacting with the microtubules, allowing for the accurate distribution of genetic material to the daughter cells. Centromeres can vary in their position and structure among different species, ranging from being located near the middle of the chromosome (metacentric) to being positioned closer to one end (acrocentric). The precise location and characteristics of centromeres are essential for proper chromosome segregation and maintenance of genomic stability.

Chromosomal proteins, non-histone, are a diverse group of proteins that are associated with chromatin, the complex of DNA and histone proteins, but do not have the characteristic structure of histones. These proteins play important roles in various nuclear processes such as DNA replication, transcription, repair, recombination, and chromosome condensation and segregation during cell division. They can be broadly classified into several categories based on their functions, including architectural proteins, enzymes, transcription factors, and structural proteins. Examples of non-histone chromosomal proteins include high mobility group (HMG) proteins, poly(ADP-ribose) polymerases (PARPs), and condensins.

Autoantigens are substances that are typically found in an individual's own body, but can stimulate an immune response because they are recognized as foreign by the body's own immune system. In autoimmune diseases, the immune system mistakenly attacks and damages healthy tissues and organs because it recognizes some of their components as autoantigens. These autoantigens can be proteins, DNA, or other molecules that are normally present in the body but have become altered or exposed due to various factors such as infection, genetics, or environmental triggers. The immune system then produces antibodies and activates immune cells to attack these autoantigens, leading to tissue damage and inflammation.

Kinetochores are specialized protein structures that form on the centromere region of a chromosome. They play a crucial role in the process of cell division, specifically during mitosis and meiosis. The primary function of kinetochores is to connect the chromosomes to the microtubules of the spindle apparatus, which is responsible for separating the sister chromatids during cell division. Through this connection, kinetochores facilitate the movement of chromosomes towards opposite poles of the cell during anaphase, ensuring equal distribution of genetic material to each resulting daughter cell.

Pulmonary Surfactant-Associated Protein B (SP-B) is a small, hydrophobic protein that is an essential component of pulmonary surfactant. Surfactant is a complex mixture of lipids and proteins that reduces surface tension at the air-liquid interface in the alveoli of the lungs, thereby preventing collapse of the alveoli during expiration and facilitating lung expansion during inspiration. SP-B plays a crucial role in the biophysical function of surfactant by promoting its spreading and stability. It is synthesized and processed within type II alveolar epithelial cells and secreted as a part of lamellar bodies, which are lipoprotein complexes that store and release surfactant. Deficiency or dysfunction of SP-B can lead to severe respiratory distress syndrome (RDS) in infants and other lung diseases in both children and adults.

Chromosome segregation is the process that occurs during cell division (mitosis or meiosis) where replicated chromosomes are separated and distributed equally into two daughter cells. Each chromosome consists of two sister chromatids, which are identical copies of genetic material. During chromosome segregation, these sister chromatids are pulled apart by a structure called the mitotic spindle and moved to opposite poles of the cell. This ensures that each new cell receives one copy of each chromosome, preserving the correct number and composition of chromosomes in the organism.

Mitosis is a type of cell division in which the genetic material of a single cell, called the mother cell, is equally distributed into two identical daughter cells. It's a fundamental process that occurs in multicellular organisms for growth, maintenance, and repair, as well as in unicellular organisms for reproduction.

The process of mitosis can be broken down into several stages: prophase, prometaphase, metaphase, anaphase, and telophase. During prophase, the chromosomes condense and become visible, and the nuclear envelope breaks down. In prometaphase, the nuclear membrane is completely disassembled, and the mitotic spindle fibers attach to the chromosomes at their centromeres.

During metaphase, the chromosomes align at the metaphase plate, an imaginary line equidistant from the two spindle poles. In anaphase, sister chromatids are pulled apart by the spindle fibers and move toward opposite poles of the cell. Finally, in telophase, new nuclear envelopes form around each set of chromosomes, and the chromosomes decondense and become less visible.

Mitosis is followed by cytokinesis, a process that divides the cytoplasm of the mother cell into two separate daughter cells. The result of mitosis and cytokinesis is two genetically identical cells, each with the same number and kind of chromosomes as the original parent cell.

Aurora Kinase B is a type of enzyme that plays a crucial role in the regulation of cell division and mitosis. It is a member of the Aurora kinase family, which includes three different isoforms (Aurora A, B, and C). Among these, Aurora Kinase B is specifically involved in the proper alignment and separation of chromosomes during cell division.

During mitosis, Aurora Kinase B forms a complex with other proteins to form the chromosomal passenger complex (CPC), which plays a critical role in ensuring accurate chromosome segregation. The CPC is responsible for regulating various events during mitosis, including the attachment of microtubules to kinetochores (protein structures that connect chromosomes to spindle fibers), the correction of erroneous kinetochore-microtubule attachments, and the regulation of the anaphase promoting complex/cyclosome (APC/C), which targets specific proteins for degradation during mitosis.

Dysregulation of Aurora Kinase B has been implicated in various human diseases, including cancer. Overexpression or amplification of this kinase can lead to chromosomal instability and aneuploidy, contributing to tumorigenesis and cancer progression. As a result, Aurora Kinase B is considered a promising target for the development of anti-cancer therapies, with several inhibitors currently being investigated in preclinical and clinical studies.

Aurora kinases are a family of serine/threonine protein kinases that play crucial roles in the regulation of cell division. There are three members of the Aurora kinase family, designated as Aurora A, Aurora B, and Aurora C. These kinases are involved in the proper separation of chromosomes during mitosis and meiosis, and their dysregulation has been implicated in various types of cancer.

Aurora A is primarily located at the centrosomes and spindle poles during cell division, where it regulates centrosome maturation, bipolar spindle formation, and chromosome segregation. Aurora B, on the other hand, is a component of the chromosomal passenger complex (CPC) that localizes to the centromeres during prophase and moves to the spindle midzone during anaphase. It plays essential roles in kinetochore-microtubule attachment, chromosome alignment, and cytokinesis. Aurora C is most similar to Aurora B and appears to have overlapping functions with it, although its specific roles are less well understood.

Dysregulation of Aurora kinases has been associated with various types of cancer, including breast, ovarian, colon, and lung cancers. Overexpression or amplification of Aurora A is observed in many cancers, leading to chromosomal instability and aneuploidy. Inhibition of Aurora kinases has emerged as a potential therapeutic strategy for cancer treatment, with several small molecule inhibitors currently under investigation in clinical trials.

Satellite DNA is a type of DNA sequence that is repeated in a tandem arrangement in the genome. These repeats are usually relatively short, ranging from 2 to 10 base pairs, and are often present in thousands to millions of copies arranged in head-to-tail fashion. Satellite DNA can be found in centromeric and pericentromeric regions of chromosomes, as well as at telomeres and other heterochromatic regions of the genome.

Due to their repetitive nature, satellite DNAs are often excluded from the main part of the genome during DNA sequencing projects, and therefore have been referred to as "satellite" DNA. However, recent studies suggest that satellite DNA may play important roles in chromosome structure, function, and evolution.

It's worth noting that not all repetitive DNA sequences are considered satellite DNA. For example, microsatellites and minisatellites are also repetitive DNA sequences, but they have different repeat lengths and arrangements than satellite DNA.

Chromosomes are thread-like structures that exist in the nucleus of cells, carrying genetic information in the form of genes. They are composed of DNA and proteins, and are typically present in pairs in the nucleus, with one set inherited from each parent. In humans, there are 23 pairs of chromosomes for a total of 46 chromosomes. Chromosomes come in different shapes and forms, including sex chromosomes (X and Y) that determine the biological sex of an individual. Changes or abnormalities in the number or structure of chromosomes can lead to genetic disorders and diseases.

Transferrin-binding protein B (TbpB) is not a medical term itself, but it is a bacterial protein involved in the process of iron acquisition by certain bacteria. Therefore, I will provide you with a biological definition:

Transferrin-binding Protein B (TbpB) is a bacterial surface protein primarily found in pathogenic Neisseria species, such as Neisseria gonorrhoeae and Neisseria meningitidis. TbpB plays a crucial role in the iron acquisition process by binding to human transferrin, a glycoprotein that transports iron in the bloodstream.

TbpB, along with Transferrin-binding Protein A (TbpA), facilitates the uptake of iron from transferrin, which is essential for bacterial growth and survival within the host. The interaction between TbpB and transferrin allows the bacteria to evade the host's immune system and establish an infection. Understanding the function of TbpB has implications in developing novel therapeutic strategies against Neisseria infections.

Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. Centromere-associated protein E is a ... Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of ... "Entrez Gene: CENPE centromere protein E, 312kDa". "OMIM Entry - # 616051 - MICROCEPHALY 13, PRIMARY, AUTOSOMAL RECESSIVE; ... 1991). "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase". EMBO J. 10 (5 ...

https://en.wikipedia.org/wiki/Centromere_protein_E

protein coding gene. Chr13:100896182-100912415 (-). 129S1/SvImJ MGP_129S1SvImJ_G0020894. protein coding gene. Chr13:102694386- ... protein coding gene. Chr13:100909370-100927500 (-). CBA/J MGP_CBAJ_G0020596. protein coding gene. Chr13:109935921-109955956 (-) ... protein coding gene. Chr13:101729367-101749082 (-). BALB/cJ MGP_BALBcJ_G0020845. protein coding gene. Chr13:99434813-99451429 ... protein coding gene. Chr13:101441831-101457671 (-). C57BL/6NJ MGP_C57BL6NJ_G0021279. protein coding gene. Chr13:105756421- ...

https://www.informatics.jax.org/marker/MGI:1349448

... Validated for WB, IF. Tested in Human. Order online. ... centromere protein C pseudogene 1 antibody, CENPCP1 antibody Background Centromere protein C 1 is a centromere autoantigen and ... Target See all Centromere Protein C Pseudogene 1 (CENPCP1) Antibodies Centromere Protein C Pseudogene 1 (CENPCP1) Reactivity ... Centromere Protein C Pseudogene 1 (CENPCP1) antibody. CENPCP1 Reactivity: Human WB, IF Host: Rabbit Monoclonal unconjugated ...

https://www.antibodies-online.com/antibody/7171191/anti-Centromere+Protein+C+Pseudogene+1+CENPCP1+antibody/

Centromere-associated protein-E (CENP-E) is an essential mitotic kinesin that is required for efficient, stable microtubule ... Centromere-associated protein-E (CENP-E) is a large (∼300 kD), essential, kinesin-like protein that accumulates in G2, is used ... Centromere-associated protein-E (CENP-E) is an essential mitotic kinesin that is required for efficient, stable microtubule ... Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single ...

https://rupress.org/jcb/article/162/4/551/33359/Centromere-associated-protein-E-is-essential-for

Phenotype data for mouse gene Cenpc1. Discover Cenpc1s significant phenotypes, expression, images, histopathology and more. Data for gene Cenpc1 is all freely available for download.

https://www.mousephenotype.org/data/genes/MGI:99700

centromere protein A. Product list Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of ... Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 ... This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting ... The protein is a replication-independent histone that is a member of the histone H3 family. ...

https://neutab.creative-biolabs.com/symbol-cenpa-2963.htm

Biotin-centromere protein B probe - 5 nmol. Cat.Number : PN-CP040-005 ... PNA FISH Centromere probes are recommended for tracking chromosome aberrations in the centromere such as trisomy and polysomy. ... The sequence of the Biotin-labeled centromere probe AAACTAGACAGAAGCATT is 5 labeled by the Biotin fluorescent Dye. The ...

https://www.eurogentec.com/en/catalog/biotin-labeled-centromere-probe-5-nmol~2cf6d10f-3b7e-48e9-b712-bcecc7b1a57f

Nuclear Proteins - Centromere Protein A PubMed MeSh Term *Overview. Overview. subject area of * CAL1 is the Drosophila CENP-A ... Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the ...

https://vivo.colorado.edu/display/meshid_D12.776.660.720.173

Human CENPF(Centromere Protein F) ELISA Kit. Human CENPF(Centromere Protein F) ELISA Kit. Order Now: [email protected] ... Description: A sandwich quantitative ELISA assay kit for detection of Human Centromere Protein F (CENPF) in samples from tissue ... Description: A sandwich quantitative ELISA assay kit for detection of Human Centromere Protein F (CENPF) in samples from tissue ... Description: A competitive ELISA for quantitative measurement of Rat Centromere protein F(CENPF) in samples from blood, plasma ...

https://kits-elisa.com/human-cenpfcentromere-protein-f-elisa-kit/

Phone: +01(619)302-0129. [email protected] ...

https://lifeome.com/recombinant-proteins-centromere-protein-c-22_230.html

We divided mice into three groups: human recombinant centromere protein-A (human CENP-A, HA) and complete Freunds adjuvant ( ... centromere protein-A) antibody had significant growth impairment and/or high mortality [12]. CENP-A is an important protein of ... Ying, Y., Liu, S., Wu, Y. et al. Anticentromere antibody induced by immunization with centromere protein a and Freunds ... We divided mice into three groups: human recombinant centromere protein-A (human CENP-A, HA) and complete Freunds adjuvant ( ...

https://rbej.biomedcentral.com/articles/10.1186/s12958-021-00737-w

Protein Product. centromere-binding protein CNN1 Feature Type. ORF , Verified Resources. AlphaFold Protein Structure , Gene/ ...

https://www.yeastgenome.org/locus/S000001942/sequence

... centromere protein I (CENPI), which is essential for mitosis [34]; apelin (APLN) that attenuates oxidative stress [35]; actinin ... ECM proteins constitute a microenvironment that provides structural support and attachment to the cells and offer essential ... L. Zhang, M. Wang, X. Kang et al., "Oxidative stress and asthma: proteome analysis of chitinase-like proteins and FIZZ1 in lung ... K. Pachmann and W. Leibold, "Insolubilization of protein antigens on polyacrylic plastic beads using poly-l-lysine," Journal of ...

https://www.hindawi.com/journals/bmri/2016/8196078/

Name: centromere protein E. Synonyms: N-7 kinesin, CENP-E, 312kDa, Kif10 ...

https://www.mmrrc.org/catalog/sds.php?mmrrc_id=40067

centromere protein J. Synonyms. Sas4,4932437H03Rik. Species. Mus musculus (mouse). Chromosome. 14. ...

https://www.mmrrc.org/catalog/locus_detail.php?mgi_id=MGI:2684927

Centromere protein A (CENP-A) is a 17 kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of ... Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone ... centromere protein ACENP-Acentromere protein A, 17kDa. *histone H3-like centromeric protein A ...

https://www.novusbio.com/products/cenpa-antibody_nbp2-92632

Centromere. The centromere appears as a constricted region of a chromosome and plays a key role in helping the cell divide up ... Protein. Proteins are large, complex molecules that play many important roles in the body. They are critical to most of the ... This can result in the addition of the wrong amino acids to the protein and/or the creation of a codon that stops the protein ... A ribosome is an intercellular structure made of both RNA and protein, and it is the site of protein synthesis in the cell. The ...

https://www.genome.gov/genetics-glossary/h

Hou H et al. Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8. Nature 591:671-676 (2021). PubMed: ... May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and ... Lysates/proteins at 20 µg per lane.. Performed under reducing conditions.. Predicted band size: 65 kDa. Observed band size: 77 ... Epigenetics and Nuclear Signaling Cell cycle Chromosome Structure Centromere Share by email ...

https://www.abcam.com/products/primary-antibodies/cenpb-antibody-ab25734.html

... basic metabolism proteins, structural proteins including many membrane proteins, and proteins required for transcription, ... There are 17-18K protein coding genes. If one assumes one gene, one protein, then I guess it implies there are 17-18K proteins ... Proteins for 85% of these genes have been detected by various assays.Over the years, the number of protein-coding genes in the ... Take any tissue or groups of cells and isolate all the proteins. Digest them with a protease-enzymes that chop the protein into ...

https://sandwalk.blogspot.com/2015/11/how-many-proteins-do-humans-make.html

The protein functions as a chaperone to help fold other proteins. They are among the most highly conserved genes in all of ... The three genes for the normal cellular proteins are HSPA5 (Bip, the ER protein); HSPA8 (the cytoplasmic version); and HSPA9 ( ... Some of them are the protein-coding genes for RNA polymerase subunits, ribosomal proteins, enzymes of lipid metabolism, and ... The "core fitness" protein-coding genes that were identified are more highly conserved than the other genes and they tend to be ...

https://sandwalk.blogspot.com/2015/12/how-many-human-protein-coding-genes-are.html?showComment=1449806985714

Most eukaryotic centromeres are defined epigenetically by the histone H3 variant, centromere protein (CENP)-A, yet how its self ... The RNA-binding protein Rbfox2: An essential regulator of EMT-driven alternative splicing and a mediator of cellular invasion ... The centromere is an essential chromosomal region required for accurate chromosome segregation. ...

https://www.researchgate.net/profile/Luciano-Rago

Protein-coding genes were predicted for each genome assembly using the MAKER2 (ref. 55) and PRAM56 pipelines. RNA evidence was ... The positions of gaps are marked with black rectangles on the chromosomes, and centromeres are represented by orange rectangles ... By way of demonstration, we considered the gene Solyc03G001472, which encodes a protein of unknown function. The cis- ... For genes mapped to the backbone without any protein-coding gene annotation, we selected the longest one if annotated in more ...

https://www.nature.com/articles/s41586-022-04808-9?error=cookies_not_supported

Home > Protein > CENPT mouse. human. rat. chicken. New Protein Search:. Centromere protein T ... Protein Mutation Frequency in Cancer. The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA ...

https://www.phosphosite.org/proteinAction.action?id=11353&showAllSites=true

... inhibits centromere protein E kinesin-like protein; structure in first source ... 11/01/2009 - "Despite a structural relationship to UA-62784, which inhibits the mitotic kinesin centromere protein E (CENP-E), ...

https://www.curehunter.com/public/keywordSummaryC535156-UA-62784.do

Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes ... MTs interact with a chromosome via the kinetochore (KC), a large protein complex located at the centromere. MTs have to get in ... Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes ... GFP provides a higher signal-to-noise ratio than the available red fluorescence proteins (e.g. tdTomato and mCherry) and a ...

https://www.nature.com/articles/srep25736?error=cookies_not_supported&code=69ac7ae6-5e12-40e1-9971-e8bff909f166

The protein was found at the nucleolus and the cytoplasm in interphase and transiently associates with centromeres in mitosis ... Gene structure, chromosomal localization and immunolocalization of chicken centromere proteins CENP-C and ZW10 ... correlates with the extent of centromere formation and provides evidence for a trans-acting centromere maintenance activity ... CENP-C is an evolutionarily conserved centromeric protein. We have used the chicken DT40 cell line to test the idea that CENP-C ...

https://www.researchgate.net/profile/Carlos-Pendon

Subcellular Location: Nucleus, Chromosome, centromere, kinetochore. Protein Families: Tissue Specificity: Paythway: Form: ... Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of ... storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80 ...

https://joplink.net/recombinant-human-zw10-interactor-zwint-csb-ep527261hu/

Using the thale cress and its mutant as their models, the scientists found the protein complexes that determine centromere ... It suggests centromere distribution in the nucleus plays a vital role in maintaining genome integrity, particularly under ... distribution during cell division and another family of proteins that stabilize the positions of the scattered centromeres on ... Scientists have uncovered a mechanism that controls how centromeres are scattered in the nucleus. ...

https://www.genengnews.com/news/centromere-distribution-governs-genome-integrity-and-stress-response/

Kinesin-like protein 6; Kinesin-like protein KIF2C; kinesin-related protein 2; MCAK; Mitotic centromere-associated kinesin; ... The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. ... microtubule motor activity protein binding ATP binding ATPase activity centromeric DNA binding microtubule plus-end binding ... Full length human recombinant protein of human KIF2C produced in HEK293T cell. View immunogen .st0{fill:#FFFFFF;} .st1{fill:# ...

https://www.thermofisher.com/antibody/product/KIF2C-Antibody-clone-OTI1C5-Monoclonal/CF503320

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. (creative-biolabs.com)
The centromere is an essential chromosomal region required for accurate chromosome segregation. (researchgate.net)
Chromosomal centromeres attached to microtubules and pulled to opposite poles of the cell during mitosis are retained in the chromatin structure even when the cell is done dividing. (genengnews.com)
The chromosomal proteins play a role in the regulation of DNA and are called the epigenome. (carnivorousplants.org)
Centromeres provide chromosomal points of attachment to the cellular segregation machinery, linking chromosomes to the proteins that pull them to the cell poles during both somatic and germline cell divisions. (jax.org)

Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. (wikipedia.org)
Kinetochores are protein complexes on the chromosomes, whose function as linkers between spindle microtubules and chromosomes is crucial for proper cell division. (nature.com)
Just like you might use a belt to keep your clothes in place, the centromere holds a pair of chromatids together and attaches it to the mitotic spindle during cell division to ensure that each daughter cell ends up with the correct number of chromosomes. (genengnews.com)
This default mode of centromere distribution is called the Rabl configuration after the 19th century cytologist Carl Rabl who was the first to note the continuity of chromosomes throughout cell division. (genengnews.com)
DNA and histone proteins are packaged into structures called chromosomes. (medlineplus.gov)

Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Centromere Protein F (CENPF) in Tissue homogenates, cell lysates and other biological fluids. (kits-elisa.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Centromere Protein F (CENPF) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (kits-elisa.com)
More recently, we found that embryos cultured with anti-CENP-A (centromere protein-A) antibody had significant growth impairment and/or high mortality [ 12 ]. (biomedcentral.com)

One of the annoying things about all three papers is that they use the words "gene" and "protein-coding gene" as synonyms. (blogspot.com)

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. (antikoerper-online.de)
Centromeres and pseudoautosomal regions (PARs) are highly specialized chromatin domains that are indispensable for proper chromosome segregation. (jax.org)
Mutations that disrupt centromere integrity or reduce homology between X- and Y-linked PARs can lead to chromosome segregation errors and constitute important genetic mechanisms for cancer, cellular senescence, and infertility. (jax.org)

This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. (creative-biolabs.com)
Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. (creative-biolabs.com)
The protein is a replication-independent histone that is a member of the histone H3 family. (creative-biolabs.com)
Centromere protein A (CENP-A) is a 17 kD centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. (novusbio.com)
Most eukaryotic centromeres are defined epigenetically by the histone H3 variant, centromere protein (CENP)-A, yet how its self-propagation is achieved remains poorly understood. (researchgate.net)

Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. (novusbio.com)
In the Rabl configuration, the free ends of the chromatids-another region of condensed chromatin called telomeres-are clustered at the opposite end of the nucleus, with respect to the centromeres. (genengnews.com)
One might expect that the position of centromeres and telomeres could influence the organization of chromatin and thereby regulate gene expression. (genengnews.com)
To their surprise, they found little difference in gene expression, indicating the robustness of chromatin organization regardless of the type of centromere distribution. (genengnews.com)

The current estimates are about 20,000 protein-coding genes and about 5,000 genes for functional RNAs. (blogspot.com)
Aside from the obvious highly conserved genes for ubiquitous RNAs (rRNA, tRNAs etc.), protein-coding genes are the easiest to recognize from looking at a genome sequence. (blogspot.com)
There are about 20,000 potential protein-coding genes in the human genome. (blogspot.com)
Proteins for 85% of these genes have been detected by various assays. (blogspot.com)
Over the years, the number of protein-coding genes in the human genome has dropped from the initial reports of about 30,000 to about 20,000. (blogspot.com)
The gene-finding algorithms tend to over-predict the number of protein-coding genes and subsequent annotation leads to a "finished" genome sequence that eliminates all the false positives. (blogspot.com)
Several thousand more genes have matched to proteins in various ways. (blogspot.com)
That still left thousand of putative genes with no evidence that they actually make a protein. (blogspot.com)
Sandwalk: How many human protein-coding genes are essential for cell survival? (blogspot.com)
The human genome contains about 20,000 protein-coding genes and about 5,000 genes that specify functional RNAs. (blogspot.com)
Some of them are the protein-coding genes for RNA polymerase subunits, ribosomal proteins, enzymes of lipid metabolism, and many other enzymes. (blogspot.com)
Each group identified between 1500 and 2000 protein-coding genes that are essential in their chosen cell lines. (blogspot.com)
The only genes they screened were protein-coding genes but the authors act as though that covers ALL genes. (blogspot.com)
2015). What they meant is that they represent 9.2% of protein-coding genes. (blogspot.com)
The characterization of new miRNA target genes and a unique profile for crasiRNAs has allowed for insight into multiple RNA mediated processes in the tammar, including gene regulation, species incompatibilities, centromere and chromosome function. (biomedcentral.com)
The location of the centromere on each chromosome gives the chromosome its characteristic shape, and can be used to help describe the location of specific genes. (medlineplus.gov)
Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
Cellular DNA-binding proteins encoded by the rel gene (GENES, REL). (bvsalud.org)

Centromere-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. (wikipedia.org)
Centromere-associated protein-E (CENP-E) is an essential mitotic kinesin that is required for efficient, stable microtubule capture at kinetochores. (rupress.org)
Bod1 depletion does not affect the activity or localization of Aurora B but does cause mislocalization of the microtubule depolymerase mitotic centromere- associated kinesin and prevents its efficient phosphorylation by Aurora B. Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles. (rupress.org)

1998). "Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E". J. Cell Biol. (wikipedia.org)
We divided mice into three groups: human recombinant centromere protein-A (human CENP-A, HA) and complete Freund's adjuvant (CFA) were used to immunize mice for the study group (HA + CFA), and mice injected with CFA (CFA group) or saline (Saline group), respectively, served as controls. (biomedcentral.com)

A family of inhibitory proteins which bind to the REL PROTO-ONCOGENE PROTEINS and modulate their activity. (bvsalud.org)

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. (thermofisher.com)
This whole package of DNA (genome) and protein (epigenome) in a chromosome is what controls the development and maintenance of eukaryotic organisms (protists, fungi, animals, and plants). (carnivorousplants.org)
in eukaryotic cells the most important of these proteins are the histones . (wikipedia.org)

Centromere protein C 1 is a centromere autoantigen and a component of the inner kinetochore plate. (antibodies-online.com)

PNA FISH Centromere probes are recommended for tracking chromosome aberrations in the centromere such as trisomy and polysomy. (eurogentec.com)

We also did selective epitope mapping using BLAST and showed similarities and homology between spike, nucleoprotein, and many other SARS-CoV-2 proteins with the human tissue antigens mitochondria M2, F-actin and TPO. (frontiersin.org)
Cross-reactivity occurs when amino acid sequence homology exists between a pathogen and self-tissue proteins ( 1 ). (frontiersin.org)

A collection of microtubules (structural proteins) called a centrosome also replicates. (coursehero.com)

This is called the non-Rabl configuration and involves an active rearrangement of centromeres and telomeres during interphase-the period between mitosis. (genengnews.com)
Once the cell has divided and entered interphase, CRWN proteins stabilize the positions of the scattered centromeres on the inner surface of the nuclear envelop, in the second step. (genengnews.com)
A cell spends most of its time in interphase, during which it mostly performs its normal functions, such as protein synthesis. (coursehero.com)

The first of these is Bod1, a protein conserved throughout metazoans that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. (rupress.org)

A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)
Therefore, we designed a protein-free dispersion media containing phospholipids and amino acids in an isotonic balanced electrolyte solution, a solution for nasal and olfactory transport (SNOT). (cdc.gov)

We sought to determine whether immune reactivity occurs between anti-SARS-CoV-2 protein antibodies and human tissue antigens, and whether molecular mimicry between COVID-19 viral proteins and human tissues could be the cause. (frontiersin.org)

Description: A sandwich quantitative ELISA assay kit for detection of Human Centromere Protein F (CENPF) in samples from tissue homogenates, cell lysates or other biological fluids. (kits-elisa.com)
Description: A competitive ELISA for quantitative measurement of Human Centromere protein F(CENPF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (kits-elisa.com)

Since their discovery, scientists have noted that the arrangement of centromeres in the nucleus between cell divisions differs among species, cell-types, cell-cycle stage, and the cell's state of specialization, but neither the underlying mechanism of their seemingly random nuclear distribution nor its biological significance was clear, until now. (genengnews.com)
If the positions of centromeres upon cell division do not change, they cluster on one side of the nucleus. (genengnews.com)
On the other hand, in some species centromeres and telomeres are evenly distributed at the periphery of the nucleus. (genengnews.com)
In the CYTOPLASM, I-kappa B proteins bind to the transcription factor NF-KAPPA B. Cell stimulation causes its dissociation and translocation of active NF-kappa B to the nucleus. (bvsalud.org)

As part of the genome sequencing initiative for the tammar, we have explored the evolution of each of the major classes of mammalian small RNAs in an Australian marsupial for the first time, including the first genome-scale analysis of the newest class of small RNAs, centromere repeat associated short interacting RNAs (crasiRNAs). (biomedcentral.com)
Despite their fundamental significance for chromosome transmission and genome stability, little is known about the levels and patterns of genetic diversity across centromeres and the PAR, or the biological impacts of this variation. (jax.org)

The mature miRNA is then loaded onto an Argonaute protein (Ago2 in humans) where it then interacts with and regulates the mRNA target. (biomedcentral.com)

Together with LINC, CII plays a role in scattering centromeres around the nuclear periphery during late anaphase, when the cell membrane invaginates, and telophase, when daughter cells physically separate during the terminal stages of cell division. (genengnews.com)
Mn, serum Mn, urine Mn, Centromere-positive micronuclei in periphery blood lymphocytes, DNA protein-crosslinks in peripheral white blood cells, oxidative stress as urinary 8OHdG, and long-term oxidative damage measured as erythrocytic superoxide dismutase (SOD) activity (measure of systemic oxidative stress) and serum malondialdehyde (MDA) (product of lipid peroxidation and reflect tissue injuries). (who.int)

The HSP70 gene family encodes the major heat shock protein of molecular weight 70,000. (blogspot.com)

If the protein is expressed in many different species then the exon sequences will be conserved and it's easy for a computer program to identify the gene. (blogspot.com)
Decreased expression of six RNA species was seen at various time points in all cell strains analyzed, these were: plasminogen activator (PLAT), centromere protein F (CPF), replication factor C (RFC3), thymidylate synthetase (TYMS), a putative mitotic checkpoint kinase (BUB1) and a gene of unknown function (GenBank accession number AI859865). (cdc.gov)

We applied both human monoclonal anti-SARS-Cov-2 antibodies (spike protein, nucleoprotein) and rabbit polyclonal anti-SARS-Cov-2 antibodies (envelope protein, membrane protein) to 55 different tissue antigens. (frontiersin.org)

Each chromosome is made up of DNA tightly coiled many times around proteins called histones that support its structure. (medlineplus.gov)

The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. (phosphosite.org)
Similarly, TP53 mutation does not always lead to the accumulation of p53 protein in cells. (spandidos-publications.com)

Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) were performed to detect the expression of p53 protein and gene deletion in ESCC tissue samples from different ethnic groups in Xinjiang, in order to analyze their association with clinicopathological characteristics and patient prognosis, as well as the sensitivity and specificity of the two methods. (spandidos-publications.com)

Using the plant Arabidopsis thaliana , also known as thale cress, as their model for non-Rabl configuration, and its mutant form with a Rabl configuration, the scientists found the protein complexes CII (condensin II) and LINC (linker of nucleoskeleton and cytoskeleton) work together to determine centromere distribution during cell division. (genengnews.com)
Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map. (thebiogrid.org)
however, even knowledge of the stoichiometry, affinity and lifetime of every protein-protein interaction would not reveal the functional relationships between and within such complexes. (thebiogrid.org)

Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

A centromere , the point on a chromosome that attaches to the spindle fibers with a kinetochore during cell division, attaches the sister chromatids. (coursehero.com)

The sequence of the Biotin-labeled centromere probe AAACTAGACAGAAGCATT is 5' labeled by the Biotin fluorescent Dye. (eurogentec.com)
Download DNA or protein sequence, view genomic context and coordinates. (yeastgenome.org)
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
The highly repetitive sequence content of these regions poses a major barrier to their molecular analysis, and the PAR and centromeres remain unassembled or incompletely assembled on many of highest quality reference genomes, including mouse. (jax.org)

Take any tissue or groups of cells and isolate all the proteins. (blogspot.com)
We found that SARS-CoV-2 antibodies had reactions with 28 out of 55 tissue antigens, representing a diversity of tissue groups that included barrier proteins, gastrointestinal, thyroid and neural tissues, and more. (frontiersin.org)
In this mechanism, antibodies formed against SARS-CoV-2 would also bind to human tissue proteins leading to autoimmune reactivity. (frontiersin.org)

Composed of tightly packaged, rapidly evolving satellite DNA with conserved functions, centromeres pose conundrums on several levels. (genengnews.com)

RNA interference (RNAi) is critical for the assembly of heterochromatin at Schizosaccharomyces pombe centromeres. (rcsb.org)

An amino acid is the fundamental molecule that serves as the building block for proteins. (genome.gov)
Each time an amino acid is added to a growing polypeptide during protein synthesis, a tRNA anticodon pairs with its complementary codon on the mRNA molecule, ensuring that the appropriate amino acid is inserted into the polypeptide. (genome.gov)
Each chromosome consists of one very long molecule of DNA twisted around proteins. (carnivorousplants.org)
These proteins, aided by chaperone proteins , bind to and condense the DNA molecule to maintain its integrity. (wikipedia.org)

The most obvious way of confirming a potential protein-coding gene is to find and confirm synthesis of a functional protein. (blogspot.com)
Defining the functional relationships between proteins is critical for understanding virtually all aspects of cell biology. (thebiogrid.org)

The detection of mutant p53 protein by immunohistochemistry (IHC) has some limitations. (spandidos-publications.com)

For example, viral infection, stress and the regulation of other proteins can also change the aggregation of p53 protein ( 20 ). (spandidos-publications.com)

For example, one of the predicted fragments of the human serum albumin protein is FKDLGEENFK (ends in lysine=K). This has an exact molecular mass of 1226.59 so if you find a peptide of that size in your mixture then it probably means that human serum albumin was present. (blogspot.com)
Full length human recombinant protein of human KIF2C produced in HEK293T cell. (thermofisher.com)

Some of them encode proteins, some of them specify abundant RNAs like tRNAs and ribosomal RNAs, some of them are responsible for making a variety of small catalytic RNAs, and some unknown fraction may specify regulatory RNAs (e.g. lncRNAs). (blogspot.com)
Using next generation sequencing, we have characterized the major classes of small RNAs, micro (mi) RNAs, piwi interacting (pi) RNAs, and the centromere repeat associated short interacting (crasi) RNAs in the tammar. (biomedcentral.com)

We believe the answer is probable, since some of the systemic disease clinical manifestations of COVID-19 cannot be explained solely by the binding of SARS-CoV-2 spike proteins with cell membranes of tissues that exhibit angiotensin-converting enzyme 2 (ACE2). (frontiersin.org)
Systemic sclerosis causes an overproduction of collagen and other proteins in various tissues. (msdmanuals.com)

In practice you want to identify several unique peptide masses for each protein just to be sure. (blogspot.com)

Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene. (wikipedia.org)
Sandwalk: How many proteins do humans make? (blogspot.com)
How many proteins do humans make? (blogspot.com)

In a cell, antisense DNA serves as the template for producing messenger RNA (mRNA), which directs the synthesis of a protein. (genome.gov)

Anatomy3

Chemicals and Drugs8

Phenomena and Processes6

Extreme reduction of chromosome-specific alpha-satellite array is unusually common in human chromosome 21. (1/99)

Uterine dysfunction and genetic modifiers in centromere protein B-deficient mice. (2/99)

Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. (3/99)

Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation. (4/99)

Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype. (5/99)

Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. (6/99)

Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B. (7/99)

Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A. (8/99)

Centromere Protein B

Centromere

Chromosomal Proteins, Non-Histone

Autoantigens

Kinetochores

Pulmonary Surfactant-Associated Protein B

Chromosome Segregation

Mitosis

Aurora Kinase B

Aurora Kinases

DNA, Satellite

Chromosomes

Transferrin-Binding Protein B

Extreme reduction of chromosome-specific alpha-satellite array is unusually common in human chromosome 21. (1/99)

Uterine dysfunction and genetic modifiers in centromere protein B-deficient mice. (2/99)

Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. (3/99)

Fission yeast homologs of human CENP-B have redundant functions affecting cell growth and chromosome segregation. (4/99)

Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype. (5/99)

Centromere/kinetochore localization of human centromere protein A (CENP-A) exogenously expressed as a fusion to green fluorescent protein. (6/99)

Functional redundancies, distinct localizations and interactions among three fission yeast homologs of centromere protein-B. (7/99)

Specification of kinetochore-forming chromatin by the histone H3 variant CENP-A. (8/99)

Centromere protein E - Wikipedia

Cenph MGI Mouse Gene Detail - MGI:1349448 - centromere protein H

Centromere Protein C Pseudogene 1 Antibody (ABIN7266273)

Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single...

Cenpc1 Mouse Gene Details | centromere protein C1 | International Mouse Phenotyping Consortium

centromere protein A - Creative Biolabs

Biotin-labeled centromere probe - 5 nmol

Nuclear Proteins - Centromere Protein A | CU Experts | CU Boulder

Human CENPF(Centromere Protein F) ELISA Kit - Enzyme-linked Immunosorbent Assay Kits

Centromere Protein- Growth Factors, Growth Hormones, Purified Proteins, Dominant Negative Clones, Constitutively Active Clones-...

Anticentromere antibody induced by immunization with centromere protein a and Freund's complete adjuvant may interfere with...

CNN1 Sequence | SGD

Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo

MMRRC:040067-MU

Cenpj

CENPA Antibody - Azide and BSA Free (NBP2-92632): Novus Biologicals

Talking Glossary of Genetic Terms | NHGRI

Anti-CENPB antibody KO Tested (ab25734) | Abcam

Sandwalk: How many proteins do humans make?

Sandwalk: How many human protein-coding genes are essential for cell survival?

Luciano RAGO | PostDoc Position | PhD | Oncogenomics | Research profile

Graph pangenome captures missing heritability and empowers tomato breeding | Nature

CENPT (mouse)

UA 62784 Summary Report | CureHunter

Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I |...

Carlos PENDON | Head of Department | Dr | Universidad de Cádiz, Cadiz | UCA | Department of Biomedicine, Biotechnology and...

Recombinant Human ZW10 interactor (ZWINT) | CSB-EP527261HU | Cusabio

Centromere Distribution Governs Genome Integrity and Stress Response

KIF2C Monoclonal Antibody (OTI1C5) (CF503320)

Chromosomal5

Chromosomes5

Antibody3

Synonyms1

Chromosome segregation3

Histone5

Chromatin4

Genes19

Kinesin3

CENP2

Proto-Oncogene1

Eukaryotic3

Autoantigen1

Recommended for tracking c1

Homology2

Structural proteins1

Interphase3

Mitosis1

Amino acids2

Viral Proteins1

CENPF2

Nucleus4

Genome2

Interacts1

Periphery2

Encodes1

Species2

Polyclonal1

Histones1

Mutation2