Recombinant human interleukin-10 inhibits proliferation of vascular smooth muscle cells stimulated by advanced glycation end products and neointima hyperplasia after carotid injury in the rat. (1/431)

The purposes of this study was to determine the effects of recombinant human interleukin-10 (rhIL-10) on proliferation of vascular smooth muscle cells (VSMCs) stimulated by advanced glycation end products (AGE) and neointima hyperplasia after rat carotid arterial injury. Rat aortic VSMCs were cultured and treated with rhIL-10 or AGE respectively, and then co-treated with rhIL-10 and AGE. Proliferation of VSMCs was quantified by colormetric assay. Cell cycle analysis was performed by flow cytomertry. Sprague-Dawley rats were treated with recombinant human IL-10 (rhIL-10) for 3 d after carotid arteries injury. The ratio of neointima to media area at the site of arterial injury was measured 28 d after balloon injury. The p44/42 MAPK activity was evaluated by the immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Compared to control, AGE stimulated VSMCs proliferation. rhIL-10 alone had no effect on VSMCs growth. With AGE stimulation, rhIL-10, at dose as low as 10 ng/ml, inhibited VSMCs growth (P<0.05). The cell number in G(0)/G(1) phase of AGE and rhIL-10 co-treatment group was higher than that of AGE treatment alone (P<0.01) by flow cytometry analysis. Compared with the control group of neointima hyperplasia in rats, the ratio of neointima to media area of recombinant human IL-10 group was reduced by 45% (P<0.01). The p44/42 MAPK activity was significantly enhanced by AGE. The AGE effects were opposed by rhIL-10. The anti-inflammatory cytokine rhIL-10 inhibits AGE-induced VSMCs proliferation. Recombinant human IL-10 also inhibited neointima hyperplasia after carotid artery injury in rats. The results suggest the possibility that recombinant human IL-10, as a potential therapeutic approach, prevents neointimal hyperplasia.  (+info)

Epidermal fatty-acid-binding protein: a new circulating biomarker associated with cardio-metabolic risk factors and carotid atherosclerosis. (2/431)

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Physical exercise, fitness and dietary pattern and their relationship with circadian blood pressure pattern, augmentation index and endothelial dysfunction biological markers: EVIDENT study protocol. (3/431)

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Medical decision-making system of ultrasound carotid artery intima-media thickness using neural networks. (4/431)

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The age correlation of the carotid intima-media thickness according to sex and side in asymptomatic subjects. (5/431)

PURPOSE: Reports concerning carotid intima-media thickness (CIMT) and linear correlation to age in healthy subjects did not distinguish the side and sex of the subjects. The purpose of this investigation attempts to clarify these issues. METHODS: 2402 asymptomatic persons, age 35-64, are separated into men's left (Lt) and right (Rt) and women's Lt and Rt carotid arteries for difference of CIMT between them and analysis of CIMT vs. age. RESULTS: There are significant difference between men's CIMT of Lt(CIMTML) vs Rt (CIMTMR), women's Lt(CIMTWL) vs. Rt (CIMTWR), Lt side CIMT of men vs women, and Rt side CIMT of men vs. women. The regression equation of CIMT vs. age for all four groups is determinated. CONCLUSION: We found an excellent linear correlation of CIMT to age and CIMT is significantly higher in men than women, so as higher in Lt than Rt. Further grouping of data into about 5-year period showed more clearly stepwise increasing of CIMT, so as the ratios of Lt CIMT different than Rt. CIMT study is served as highly efficient examination in therapy, prevention, clinic, or research survey about atherosclerosis and risk of stroke. Future study design concerning CIMT in separation groups of men and women, so as Lt and Rt is highly recommended.  (+info)

Atherogenic lipid profile and systolic blood pressure are associated with carotid artery intima-media thickness in children with Turner syndrome. (6/431)

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Polymorphic variation in choline transporter gene (CHT1) is associated with early, subclinical measures of carotid atherosclerosis in humans. (7/431)

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Real-time co-registration using novel ultrasound technology: ex vivo validation and in vivo applications. (8/431)

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