The bone-healing effect of a xenograft in a rat calvarial defect model.
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Bone grafts have been widely used to fill osseous defects in medicine, dentistry, and periodontology. The purpose of this study was to investigate the effects of a xenograft (Unilab Surgibone) on experimentally created parietal bone defects in rats. To this end, 14 rats were employed in the present study and in each of them, 5-mm-diameter defects were created on the parietal bone. The right defect sites were filled with the xenograft material, while the left sites were used as control. After 30 days, the rats were sacrificed and tissue samples were retrieved from the defect sites of the cranium. Dense collagenous tissue was observed in the control area, whereas the xenograft particles were surrounded by a fibrous tissue layer at the implantation site. Based on the findings obtained, it could be concluded that the investigated xenograft seemed biocompatible and could be proposed as a potential material for filling osseous defects. (+info)
Immunohistochemical analysis of osteoconductivity of beta-tricalcium phosphate and carbonate apatite applied in femoral and parietal bone defects in rats.
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The feature of osteoconductivity, and expression of inductive BMP and transcription factors (Runx2 and Osterix) for osteoblast differentiation, which was related to conductive bone formation, were observed in experimentally created defects in rat femoral and parietal bones filled with beta-tricalcium phosphate (beta-TCP) or carbonate apatite (CAP). Femoral cortical bone defects were repaired by conductive bone formed by osteoblasts differentiated around beta-TCP and CAP, and immunohistochemical observation revealed that the osteoblasts expressed BMPs, Runx2, and Osterix. However, the repair in parietal bone defects was incomplete despite the beta-TCP and CAP filling. Only cells, which differentiated around beta-TCP or CAP, and formed conductive bone expressed BMPs, Runx2, and Osterix. These findings revealed that the osteoconductivity of calcium phosphate materials required the expression of BMPs as the prerequisite for Runx2 and Osterix expression. Therefore, it is suggested that when calcium phosphate ceramics are used as bone substitute materials, BMPs are essential for osteoconductivity. (+info)
Origin matters: differences in embryonic tissue origin and Wnt signaling determine the osteogenic potential and healing capacity of frontal and parietal calvarial bones.
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