This adenylated ubiquitin is then transferred to a cysteine of a second enzyme, ubiquitin-conjugating enzyme (E2). Lastly, a ... Ubiquitin-activating enzyme: Mechanism and role in protein-ubiquitin conjugation. J Biol Chem 1982 Mar 10;257(5):2543-8. PMID ... In the first step, a ubiquitin-activating enzyme (known as E1) hydrolyzes ATP and adenylates a ubiquitin molecule. This is then ... The most well-known example of a ubiquitin-independent proteasome substrate is the enzyme ornithine decarboxylase. Ubiquitin- ...
Characterization of a temperature-sensitive mutant of a ubiquitin-conjugating enzyme and its use as a heat-inducible ... Le rapporteur de repliement, constitué d'une protéine modèle pour l'agrégation, était un Ubc9 (enzyme SUMO) mutant (UBC9ts), ... A yeast Ubc9 mutant protein with temperature-sensitive in vivo function is subject to conditional proteolysis by a ubiquitin- ...