Purification of rutin and nicotiflorin from the flowers of Edgeworthia chrysantha Lindl. by high-speed counter-current chromatography.
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An ethanol extract of air-dried flowers of Edgeworthia chrysantha Lindl. was partitioned between water and petroleum, ethyl acetate, and n-butanol. The n-butanol extraction was initially purified by silica gel column chromatography to give a partially purified sample. The bioactive compound rutin, along with nicotiflorin, were successfully separated from the partially purified sample by high-speed counter-current chromatography. The two compounds were isolated from the plant of Edgeworthia genus for the first time. The two-phase solvent system used was composed of ethyl acetate-n-butanol-water at an optimized ratio of 4:1:5 (v/v/v). High-speed counter-current chromatography yielded, from 108 mg of the partially purified extract, 53 mg rutin and 32 mg nicotiflorin with 92.5% and 92.2% recovery, with each at over 96.5% purity by high-performance liquid chromatography analysis. Their structures were identified by 1H NMR and 13C NMR. (+info)
Allopurinol, rutin, and quercetin attenuate hyperuricemia and renal dysfunction in rats induced by fructose intake: renal organic ion transporter involvement.
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Effect of pregnancy and tobacco smoke on the antioxidant activity of rutin in an animal model.
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Tobacco smoke is a source of free radicals and causes oxidative stress in smokers' tissues. The aim of the current study was to evaluate the effect of rutin on the total antioxidant status (TAS) in pregnant and non-pregnant rats that were exposed to cigarette smoke. TAS in brain, lungs, liver, kidneys and plasma were measured by the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical-cation decolorization assay. In pregnant rats, a diversified distribution of endogenous antioxidants was found in comparison to the matched non-pregnant animals. In pregnant rats, TAS was higher in plasma (by 33%) and kidney (by 76%), and lower in brain (by 48%) and liver (by 50%) compared with non-pregnant rats. Generally (except liver), exposure to tobacco smoke caused an increase in the antioxidative status of pregnant compared to non-pregnant animals (by 29, 16, 18 and 87% in plasma, brain, lung and kidney, respectively). Overall, rutin had little (plasma, non-pregnant rats) or a no protective effect in the examined tissues. (+info)
Study of efficacy and safety of a new local cream ('healer') in the treatment of chronic anal fissure: a prospective, randomized, single-blind, comparative study.
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Application of the MSPD technique for the HPLC analysis of rutin in Sambucus nigra L.: the linear correlation of the matrix solid-phase dispersion process.
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This paper presents possibilities of estimating the true value of an analyte concentration in a plant material by means of the matrix solid-phase dispersion (MSPD) based on the law of the partition process and on the properly adapted equations associated with this process. This idea has been developed for analysis of rutin amount in Sambucus nigra L. (elderberry). The effects of experimental variables, such as the mass ratio of sorbent to plant matrix and buffer pH, have been studied. Rutin amounts estimated using MSPD were verified with the amounts obtained by employing pressurized liquid extraction (PLE). The extracts were analyzed by high-performance liquid chromatography (HPLC). To obtain the true value of rutin concentration in elderberry, the MSPD process should be carried out in the conditions for which the dependence between the reciprocal of the analyte yield and sorbent mass/plant mass ratio is linear. Rutin yield estimated by MSPD in such conditions equal 2.90% w/w and is almost the same as the amount obtained by PLE (2.88% w/w). The results show that PLE, which recently has been more and more frequently used in the analysis of herb material and which requires the application of expensive PLE equipment, can be replaced by the relatively cheap and effective MSPD process. (+info)