Evaluating bacterial pathogen DNA preservation in museum osteological collections.
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Reports of bacterial pathogen DNA sequences obtained from archaeological bone specimens raise the possibility of greatly improving our understanding of the history of infectious diseases. However, the survival of pathogen DNA over long time periods is poorly characterized, and scepticism remains about the reliability of these data. In order to explore the survival of bacterial pathogen DNA in bone specimens, we analysed samples from 59 eighteenth and twentieth century individuals known to have been infected with either Mycobacterium tuberculosis or Treponema pallidum. No reproducible evidence of surviving pathogen DNA was obtained, despite the use of extraction and PCR-amplification methods determined to be highly sensitive. These data suggest that previous studies need to be interpreted with caution, and we propose that a much greater emphasis is placed on understanding how pathogen DNA survives in archaeological material, and how its presence can be properly verified and used. (+info)
Wolbachia infections in the Cimicidae: museum specimens as an untapped resource for endosymbiont surveys.
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Wolbachia spp. are obligate maternally inherited endosymbiotic bacteria that infect diverse arthropods and filarial nematodes. Previous microscopic and molecular studies have identified Wolbachia in several bed bug species (Cimicidae), but little is known about how widespread Wolbachia infections are among the Cimicidae. Because cimicids of non-medical importance are not commonly collected, we hypothesized that preserved museum specimens could be assayed for Wolbachia infections. For the screening of museum specimens, we designed a set of primers that specifically amplify small diagnostic fragments (130 to 240 bp) of the Wolbachia 16S rRNA gene. Using these and other previously published primers, we screened 39 cimicid species (spanning 16 genera and all 6 recognized subfamilies) and 2 species of the sister family Polyctenidae for Wolbachia infections using museum and wild-caught material. Amplified fragments were sequenced to confirm that our primers were amplifying Wolbachia DNA. We identified 10 infections, 8 of which were previously undescribed. Infections in the F supergroup were common in the subfamily Cimicinae, while infections in the A supergroup were identified in the subfamilies Afrocimicinae and Haematosiphoninae. Even though specimens were degraded, we detected infections in over 23% of cimicid species. Our results indicate that Wolbachia infections may be common among cimicids and that archived museum material is a useful untapped resource for invertebrate endosymbiont surveys. The new screening primers listed in this report will be useful for other researchers conducting Wolbachia surveys with specimens with less-than-optimum DNA quality. (+info)
The art of observation: impact of a family medicine and art museum partnership on student education.
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BACKGROUND AND OBJECTIVES: Compared to verbal communication, teaching the skill of observation is often shortchanged in medical education. Through a family medicine-art museum collaboration, we developed an elective course for second-year medical students titled the "Art of Observation" (AOO). To evaluate the course's effect on clinical skills, we performed a qualitative evaluation of former students during their clinical rotations. METHODS: In the spring of 2005, all students who had completed the AOO course in 2003 or 2004 were invited to take part in an online evaluation consisting of eight journaling survey questions. Students were instructed to answer the survey questions with specific examples. Question areas included the most memorable experience, the course's influence on the doctor-patient relationship, usefulness during clinical years of medical school, and skills unique to AOO. The anonymous data were analyzed qualitatively, coding the responses to categories derived from the data, leading to the formation of themes. RESULTS: Of the 19 students eligible, 17 participated. We found three important themes: (1) the AOO positively influenced clinical skills, (2) both art museum exercises and a clinical preceptorship were necessary to achieve those skills, and (3) the AOO led to a sense of personal development as a physician. In addition, students told us that the training in observation and description skills they learned were unique to the AOO. CONCLUSIONS: This collaboration between a department of family medicine and an art museum produced a course that facilitated observational skills used in successful doctor-patient relationships. (+info)
Role of anatomy in our contemporary age and the history of the Anatomy Museum of Naples.
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This work analyzes the significance of anatomical knowledge in the contemporary age through the history of a prestigious institution, the Anatomy Museum of Naples. The museum's past was ineluctably linked not only to the local sociopolitical events but also to the scientific developments of medicine. The museum is an academic place where the importance of the anatomic science in the contemporary age both in the scientific and in the cultural fora is evident. (+info)
Unusual foreign metallic object (nail) in the dentition of a skull from the anthropological collection of Rudolf Virchow (Berlin).
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Foreign bodies in the dentition of present day patients are frequently diagnosed. They are more rare in mediaeval and anthropological specimens. Rudolf Virchow, the doyen of pathology in Germany formed a huge collection of anthropological specimens in the 19th century. Among these specimens one skull from Tiflis (Tbilisi, Georgia) found its way into the collection of Virchow in 1881. The skull is that of a prisoner of war who died in 1877 due to dysentery. The skull is remarkable in that a metallic nail was adapted around the second right maxillary molar. Both radiological and clinical findings indicate that the nail was adapted to the tooth while the individual was still alive. In particular, erosion of the cortical bone plate in the affected area and osseous healing between the first and second maxillary molar may be taken as proof of adaptation of the nail in vivo. The reasons why the nail was applied, however, are difficult to explain. The authors assume that the nail was applied not by the individual himself. Probably, the nail was adapted as an amulet to protect the individual from injury or death. (+info)
Middle Paleolithic shell beads in Israel and Algeria.
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Perforated marine gastropod shells at the western Asian site of Skhul and the North African site of Oued Djebbana indicate the early use of beads by modern humans in these regions. The remoteness of these sites from the seashore and a comparison of the shells to natural shell assemblages indicate deliberate selection and transport by humans for symbolic use. Elemental and chemical analyses of sediment matrix adhered to one Nassarius gibbosulus from Skhul indicate that the shell bead comes from a layer containing 10 human fossils and dating to 100,000 to 135,000 years ago, about 25,000 years earlier than previous evidence for personal decoration by modern humans in South Africa. (+info)
Translational mini-review series on vaccines: The Edward Jenner Museum and the history of vaccination.
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Edward Jenner's discovery of vaccination must rank as one of the most important medical advances of all time and is a prominent example of the power of rational enquiry being brought to bear during the Age of Enlightenment in 18th century Europe. In the modern era many millions of lives are saved each year by vaccines that work essentially on the same principles that were established by Edward Jenner more than 200 years ago. His country home in Berkeley, Gloucestershire, is where he carried out his work and where he spent most of his life. The building is now a museum in which the life and times of Jenner are commemorated including not only the discovery of smallpox vaccination but also his other important scientific contributions to natural history and medicine. The trustees of the Edward Jenner museum are committed to promoting the museum as a real and "virtual" educational centre that is both entertaining and informative. (+info)
Non-formal education for schistosomiasis prevention: the experience of the Museu Arqueologico de Central, Bahia, Brazil.
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The activities described here form part of an extensive programme in place in the Regiao Arqueologica de Central, state of Bahia, Brazil. After malacological and socio-environmental surveys in the area, a strategy comprising formal and non-formal education with an emphasis on schistosomiasis prevention was developed, introduced, and evaluated. Interviews were conducted of 142 students and 11 teachers, totalling 11 classes at six primary schools. On the basis of those interviews, four display cases and seven panels were prepared. In addition a table was set up where students could participate directly on the subject, drawing and recognising the factors involved in the schistosomiasis cycle. The exhibition was held at the Museu Arqueologico de Central. The endeavours of this paper underline the importance of health education as well as exhibitions to disease prevention activities. (+info)