ElectrophoreticSerum protein electroCHROMATOGRAPHYElectrochromatographyCharacterizationSeparationsDetectionIsotachophoresisDeterminationMonoclonalProteinAnalytesAqueousElectrokineticallySPEPAnalyticalProteinsQuantitationSeparation methodsPolyacrylamideBufferInstrumentationDiameterIonizationHighAnalysisMethodsConcentrationGeneralTechniqueStrokeSystemPAGEComponentsResolutionNegativeContentEditMediaSamples
Electrophoretic4
- Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar electrokinetic chromatography (MEKC) belong also to this class of methods. (wikipedia.org)
- The analytes separate as they migrate due to their electrophoretic mobility, and are detected near the outlet end of the capillary. (wikipedia.org)
- This capillary zone electrophoresis (CZE) system integrates micro-capillary electrophoretic technology into a plug-and-play disposable pen-shaped multi-capillary gel cartridge with automated liquid handling (96-samples) and real-time fluorescent detection and analysis. (genengnews.com)
- It combines the simplest capillary electrophoresis mode where separations are based on the differences in the electrophoretic migration of charged analytes under the influence of a high electric field with separation based on analyte partitioning between the mobile phase and stationery phase from liquid chromatography. (shu.ac.uk)
Serum protein electro6
- An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. (degruyter.com)
- Serum protein electrophoresis (SPEP) is used to quantify the serum monoclonal component or M-protein, for diagnosis and monitoring of monoclonal gammopathies. (degruyter.com)
- Due to the wide range of disease presentations, identification of the M-protein by serum protein electrophoresis (SPEP) may be the first clue to the diagnosis of a monoclonal gammopathy followed by subsequent isotyping via immunofixation (IFE) or immunosubtraction (ISUB). (degruyter.com)
- Serum protein electrophoresis (SPEP) is an easy, inexpensive method of separating proteins based on their net charge, size, and shape. (medscape.com)
- [ 1 ] Globulins comprise a much smaller fraction of the total serum protein but represent the primary focus of interpretation of serum protein electrophoresis. (medscape.com)
- Monoclonal pattern serum protein electrophoresis (SPEP). (medscape.com)
CHROMATOGRAPHY10
- Two original products, Eprex (epoetin alfa) and Dynepo (epoetin delta), and two biosimilar products, Binocrit (epoetin alfa) and Retacrit (epoetin zeta), were compared using (1) high performance size exclusion chromatography, (2) ELISA, (3) SDS-PAGE, (4) capillary zone electrophoresis and (5) in-vivo potency. (springer.com)
- Because a limited amount of EPO products was available, we were restricted to the common assays used for quality control: high performance size exclusion chromatography (HP-SEC) to assess monomer and aggregate content, ELISA to determine EPO protein content, SDS-PAGE under non-reducing conditions to detect possible fragmentation and covalent protein aggregates, capillary zone electrophoresis (CZE) for isoform profiling and a normocythemic mouse assay to test for potency. (springer.com)
- Recent advances have stimulated interest concerning the development of microcolumn liquid-phase separation methods, including capillary liquid chromatography in various forms, and capillary zone electrophoresis. (psu.edu)
- Oligosaccharide fingerprinting employing capillary gas chromatography with flame ionization detection. (chipsbooks.com)
- Comparison of high performance liquid chromatography with capillary zone electrophoresis. (cdc.gov)
- Beckman Coulter P/ACE MDQ analyzer allows to perform all basic modes of capillary electromigration techniques, zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography. (uochb.cz)
- Currently, the expert's research focuses on applied aspects of ion chromatography, liquid chromatography -mass spectrometry and capillary zone electrophoresis in the fields of analytical and bioanalytical chemistry. (chromatographytoday.com)
- It is a hybrid technique of high performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE). (shu.ac.uk)
- 35] T. J. Kasper, M. Melera, P. Gozel and R. G. Brownlee, "Separation and detection of DNA by capillary electrophoresis", J. Chromatography, 458 1988 303-312. (rosannajohnsson.com)
- 36] M. Zhu , D. L. Hansen, S. Burd and F. Gannon , "Factors affecting free zone electrophoresis and isoelectric focusing in capillary electrophoresis", J. Chromatography, 480 1989 311-319. (rosannajohnsson.com)
Electrochromatography2
- The thesis focuses on the development of new homogeneous gels for the separation of drug enantiomers, peptides, DNA and virus by electrophoresis and electrochromatography in capillaries and microchips. (dissertations.se)
- Capillary Electrochromatography (CEC) is one of the newest separation techniques. (shu.ac.uk)
Characterization1
- 2018 ) Complementary middle-down and intact monoclonal antibody proteoform characterization by capillary zone electrophoresis - mass spectrometry. (academictree.org)
Separations1
- Native protein separations by capillary gel electrophoresis are achieved utilizing linear acrylamide gel matrices. (expressionpathology.com)
Detection6
- Capillary zone electrophoresis was employed for the determination of myoglobin in human urine using end-column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.80 V vs. saturated calomel electrode (SCF). (unboundmedicine.com)
- Certain aspects of the instrumentation (such as detection) are necessarily more complex than for a single-capillary system, but the fundamental principles of design and operation are similar to those shown in Figure 1. (wikipedia.org)
- Separation by capillary electrophoresis can be detected by several detection devices. (wikipedia.org)
- In these systems, a section of the capillary itself is used as the detection cell. (wikipedia.org)
- The portion of the capillary used for UV detection, however, must be optically transparent. (wikipedia.org)
- The path length of the detection cell in capillary electrophoresis (~ 50 micrometers) is far less than that of a traditional UV cell (~ 1 cm). (wikipedia.org)
Isotachophoresis1
- A process called field-amplified sample stacking (a form of isotachophoresis) results in concentration of analyte in a narrow zone at the boundary between the low-conductivity sample and the higher-conductivity running buffer. (wikipedia.org)
Determination1
- Capillary electrophoresis can be used as a modern analytical separation technique for the separation and determination of metal ions, inorganic anions, chiral compounds, peptides and proteins, carbohydrates, and even whole cells and viruses. (pte.hu)
Monoclonal1
- In a case report of one patient with POEMS syndrome,[5] serum electrophoresis demonstrated an M-band with isolated IgA heavy chain but no abnormal light chain, which could suggest abnormal secretion of monoclonal protein or the rare possibility of coincidental heavy-chain disease in association with POEMS syndrome. (medscape.com)
Protein2
- Analysis of Proteins, Protein Complexes, and Organellar Proteomes Using Sheathless Capillary Zone Electrophoresis - Native Mass Spectrometry. (supplementwarehouse.com)
- Instituto de Investigación en the protein rOmpA. (cdc.gov)
Analytes2
- Despite the very small sample sizes (typically only a few nanoliters of liquid are introduced into the capillary), high sensitivity and sharp peaks are achieved in part due to injection strategies that result in a concentration of analytes into a narrow zone near the inlet of the capillary. (wikipedia.org)
- In order to better understand the interaction mechanism between the selected CD and the analytes, the classical affinity capillary electrophoresis method, although not fully satisfactory because of ionic strength variations within a series of mobility shift measurements, was applied to estimate complexation constants and complex mobilities. (unboundmedicine.com)
Aqueous2
- An acid dissociation constant (p K a ) of bupropion has been determined in an aqueous solution by capillary zone electrophoresis (CZE). (go.jp)
- The source vial, destination vial and capillary are filled with an electrolyte such as an aqueous buffer solution. (wikipedia.org)
Electrokinetically2
- Sample is introduced into the capillary via capillary action, pressure, siphoning, or electrokinetically, and the capillary is then returned to the source vial. (wikipedia.org)
- We coupled capillary zone electrophoresis (CZE) with an ultrasensitive electrokinetically pumped nanospray ionization source for tandem mass spectrometry (MS/MS) analysis of complex proteomes. (analyteguru.com)
SPEP1
- A panel of assays combining SPEP with IFE/ISUB, serum free light chains (FLC), and urine electrophoresis and IFE/ISUB has the highest degree of clinical sensitivity for the diagnosis of these disorders [ 6 ]. (degruyter.com)
Analytical2
- During the decade, capillary electrophoresis has been constructed right into a very robust analytical process, which has many merits over traditional slab gel ideas. (rosannajohnsson.com)
- As capillary electrophoresis could be utilized to many alternative analytical difficulties, a spe- cialization is unavoidable. (rosannajohnsson.com)
Proteins1
- Various disease states or conditions alter the pattern of proteins in electrophoresis (see Table 1 below). (medscape.com)
Quantitation1
- A free zone capillary electrophoresis method was developed for the quantitation of cocaine in illicit powders. (astm.org)
Separation methods1
- Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels. (wikipedia.org)
Polyacrylamide1
- 4. And a support for the buffer such as filter paper, cellulose acetate strips, polyacrylamide gel, or a capillary tube. (slideshare.net)
Buffer2
- The type and the concentration of the chiral selector added to the BGE, the buffer pH, the type of organic modifier and its concentration, the capillary temperature and the applied voltage played a very important role in the enantioresolution of the analysed compounds. (unich.it)
- Using capillary zone electrophoresis with a phosphate buffer at pH 2.5 containing 30 mM (2-hydroxypropyl)-beta-cyclodextrin as chiral selector, the simultaneous separation of the enantiomers of 3,4-methylenedioxymethamphetamine (MDMA or Ecstasy) and its two metabolites 4-hydroxy-3-methoxymethamphetamine (HMMA) and 3,4-methylenedioxyamphetamine (MDA) in human urine is reported. (erowid.org)
Instrumentation2
- The instrumentation needed to perform capillary electrophoresis is relatively simple. (wikipedia.org)
- In between 1950 to 1970, an enumeration of techniques and instrumentation for Electrophoresis were developed. (slideshare.net)
Diameter1
- The assay described is based upon enzymatic hydrolysis of conjugated HMMA (major urinary metabolite) and solid-phase extraction followed by injection of a few nL of the extract onto a 50 microm internal diameter (ID) fused-silica capillary of 60 cm length. (erowid.org)
Ionization1
- Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry for quantitative parallel reaction monitoring of peptide abundance and single-shot proteomic analysis of a human cell line. (analyteguru.com)
High6
- The system's main components are a sample vial, source and destination vials, a capillary, electrodes, a high voltage power supply, a detector, and a data output and handling device. (wikipedia.org)
- Capillary electrophoresis was first combined with mass spectrometry by Richard D. Smith and coworkers, and provides extremely high sensitivity for the analysis of very small sample sizes. (wikipedia.org)
- Such capillary array electrophoresis (CAE) instruments with 16 or 96 capillaries are used for medium- to high-throughput capillary DNA sequencing, and the inlet ends of the capillaries are arrayed spatially to accept samples directly from SBS-standard footprint 96-well plates. (wikipedia.org)
- In 1955, Oliver Smithies found that separation of human tissue extracts with high resolution by starch gel electrophoresis. (slideshare.net)
- This type of separation media offers high resolution and small zone broadening. (dissertations.se)
- It is crucial to maintain a stable electrical contact throughout the CE capillary and ion-source as well as adequate grounding of the high voltage applied in CE. (shu.ac.uk)
Analysis1
- Analysis of Nucleic Acids by Capillary Electrophoresis by Christoph Heller (auth. (rosannajohnsson.com)
Methods1
- The course presents six different methods of capillary electrophoresis, starting with the theoretical foundations and explaining various pharmaceutical applications. (pte.hu)
Concentration1
- All the studied compounds were enantioresolved employing a capillary column packed with vancomycin stationary phase by nano-LC, and the resolution was strongly influenced by the concentration of the organic modifier and by the pH of the mobile phase. (unich.it)
General2
- In general, capillaries used in capillary electrophoresis are coated with a polymer (frequently polyimide or Teflon) for increased flexibility. (wikipedia.org)
- The main practical problem is the great mismatch in flow rates through the CE capillary and the solvent flow required for the general LC-MS ion-sources, such as electrospray. (shu.ac.uk)
Technique1
- The latter was achieved with partial filling technique using ultrashort separation zones (a few mm). (unboundmedicine.com)
Stroke1
- and proximal arterial stenosis with hypotension that decreases cerebral blood flow in arterial watershed zones (hemodynamic stroke). (msdmanuals.com)
System1
- A basic schematic of a capillary electrophoresis system is shown in figure 1. (wikipedia.org)
PAGE1
- C. Thin layer Electrophoresis and D. Gel Electrophoresis (SDS, PAGE, etc..) 2. (slideshare.net)
Components1
- Components of Electrophoresis unit 1. (slideshare.net)
Resolution1
- The possibility to enhance resolution to infinite value in chiral capillary electrophoresis is attained as soon as the apparent mobility of one enantiomer becomes opposite to the other. (unboundmedicine.com)
Negative1
- In the most common mode of CE, all ions, positive or negative, are pulled through the capillary in the same direction by electroosmotic flow. (wikipedia.org)
Content1
- Non-toxic low sugar content so suitable for electrophoresis of polysaccharides. (slideshare.net)
Edit1
- I greatly welcome the choice of the sequence editor and the writer, to edit a sequence ofspecialized books, overlaying all elements of capillary electrophoresis. (rosannajohnsson.com)
Media1
- Zone Electrophoresis (on supporting media) A. Paper Electrophoresis, B. Cellulose Electrophoresis. (slideshare.net)
Samples1
- To achieve greater sample throughput, instruments with arrays of capillaries are used to analyze many samples simultaneously. (wikipedia.org)