• Zeranol is a non‑steroidal agent with potent estrogenic activity, which is widely used as a growth promoter for cattle in certain countries. (spandidos-publications.com)
  • Zeranol is a non-steroidal estrogen agonist that is approved for use as a growth promoter in livestock, including beef cattle, in various countries. (spandidos-publications.com)
  • Zeranol (α-zearalanol) is a metabolite of the mycoestrogen zearalenone, and is used as a growth promoter of livestock due to its strong estrogenic activity. (elsevierpure.com)
  • Zearalenone is metabolically transformed to α-zearalenol (α-Zel) or (α-Zol), β-zearalenol (β-Zel) or (β-Zol), α-zearalanol (α-Zal), β-zearalanol (β-Zal), and zearalanone (ZAN) in animals. (wikipedia.org)
  • The immunoaffinity column can selectively adsorb Zearalenone (ZEN),Deoxynivalenol(DON) and Ochratoxin A (OTA) from the sample solution, thereby having a highly targeted purification effect on the sample. (mzfoodtest.com)
  • Zearalenone is the primary toxin that binds to estrogen receptors, causing infertility, abortion or other breeding problems, especially in swine. (wikipedia.org)
  • In addition to its actions on the classical estrogen receptors, zearalenone has been found to act as an agonist of the GPER (GPR30). (wikipedia.org)
  • Zearalenone structure is similar to estrogens and α-zearalenol binds with an even greater affinity estrogen receptors, while β-zearalenol's affinity is lower than both the parent compound's and α-Zel's binding affinity. (wikipedia.org)
  • The effects of zeranol on cell proliferation were measured using the MTS assay, aromatase expression levels were determined by immunocytochemical staining and reverse transcription‑polymerase chain reaction, and aromatase enzyme activity and estrogen production were analyzed using corresponding assay kits. (spandidos-publications.com)
  • The results demonstrated that low dose zeranol (2‑50 nM) was able to significantly promote cell proliferation, aromatase mRNA expression, aromatase activity and estrogen production in primary cultured human breast preadipocytes, thus suggesting that zeranol may act as an aromatase activator. (spandidos-publications.com)
  • The findings of the present study suggest that zeranol promotes breast cancer cell growth by stimulating aromatase activation and increasing estrogen biosynthesis in adipose tissue. (spandidos-publications.com)
  • A previous report demonstrated that zeranol is comparable to natural 17β-estradiol (E2) and the synthetic estrogen diethylstilbestrol in its ability to transform MCF-10A normal human breast epithelial cells to a pre-cancerous phenotype in vitro ( 17 ). (spandidos-publications.com)
  • Zeranol (Zer) is a semi-synthetic derivative of Zearalenone, a myco-estrogen produced by Fusarium fungi, that is used in livestock to enhance meat production. (rutgers.edu)
  • In the present study, we investigated the effects of zeranol on the growth of estrogen receptor (ER)-positive and -negative human breast carcinoma cells in vitro, and the molecules involved. (elsevierpure.com)
  • Several Fusarium species produce toxic substances of considerable concern to livestock and poultry producers, namely deoxynivalenol, T-2 toxin, HT-2 toxin, diacetoxyscirpenol (DAS) and zearalenone. (wikipedia.org)
  • The authors conclude that workers have a considerable exposure to zeranol which poses a potential health risk. (cdc.gov)
  • The present study hypothesized that aromatase expression and activity may be elevated by low dose zeranol exposure, providing a source of estrogens that may stimulate cell proliferation. (spandidos-publications.com)
  • Wipe samples showed zeranol concentrations up to 14mg per sample. (cdc.gov)
  • At low concentrations, zeranol accelerated the growth of ER-positive MCF-7 and KPL-1 human breast carcinoma cells, but did not affect the growth of ER-negative MDA-MB-231 cells. (elsevierpure.com)
  • At high concentrations, zeranol suppressed the growth of both ER-positive and -negative human breast carcinoma cells. (elsevierpure.com)
  • Thus, zeranol exerted dose-dependent biphasic effects on ER-positive human breast carcinoma cells, accelerating cell growth at low concentrations and inducing apoptosis at high concentrations. (elsevierpure.com)
  • The acceleration of ER-positive cell growth by low-dose zeranol involved the down-regulation of p21 Cip1 (a cyclin-dependent kinase inhibitor), which resulted in cell cycle progression. (elsevierpure.com)
  • Environmental and breathing zone samples were analyzed for zeranol (26538443) at Manufacturing Chemists, Incorporated (SIC-2879, SIC- requested by the State Industrial Hygiene Compliance Section to assist in evaluating the occurrence of breast symptoms, weight gain, and gynecomastia in employees and their children. (cdc.gov)
  • Additionally, in the organs of animals these metabolic products are further modified to yield zearalenone-14-glucuronide (ZEN-14GlcA), α-zearalenol-glucuronide (α-Zel-14G) and β-zearalenol-glucuronide (β-Zel-14G). (wikipedia.org)
  • Zearalenone has two major phase I metabolites: α-zearalenol and β-zearalenol. (wikipedia.org)
  • Blood samples were analyzed for zeranol metabolites. (cdc.gov)
  • No zeranol metabolites were found in blood samples. (cdc.gov)
  • With widespread human dietary exposure and growing experimental evidence of endocrine-disrupting properties, a comprehensive review of the impact of ZEN, zeranol, and their metabolites on the female reproductive system is warranted. (nih.gov)
  • The objective of this systematic review was to summarize the in vitro, in vivo, and epidemiological literature and evaluate the potential impact of ZEN, zeranol, and their metabolites (commonly referred to as mycoestrogens) on female reproductive outcomes. (nih.gov)
  • Similar to the clenbuterol contamination issue, the unintentional intake of the mycotoxin zearalenone can result in AAFs concerning one of its human urinary metabolites referred to as zeranol, b pharmaceuticals anavar. (holistichedges.com)
  • The mycoestrogen zearalenone (ZEN), a toxic fungal secondary metabolite and mycotoxin found frequently in food and feed, constitutes a prominent example. (nih.gov)
  • Among important mycoestrogens are zearalenone, zearalenol and zearalanol. (wikipedia.org)
  • Although all of these can be produced by various Fusarium species, zearalenol and zearalanol may also be produced endogenously in ruminants that have ingested zearalenone. (wikipedia.org)
  • More than one study has shown that detectable levels of zearalenone and its metabolite alpha-zearalanol in girls are associated with significantly shorter heights at menarche. (wikipedia.org)
  • Radioimmunoasssay for zearalenone and zearalanol in human serum: production, properties and use of porcine antibodies. (zju.edu.cn)
  • Zearalenone (ZEN), an estrogenic mycotoxin produced by Fusarium fungi, is a common contaminant of cereal grains and has also been detected at lower levels in meat, milk, and spices. (nih.gov)
  • Zearalenone, produced by various Fusarium species, is a non-steroidal estrogenic mycotoxin that contaminates cereals, resulting in adverse effects on human health. (nih.gov)
  • Zeranol concentrations in urine of sheep and cattle implanted with zeranol (Ralgro). (zju.edu.cn)
  • Zeranol is a non-steroidal anabolic agent administered subcutaneously as an ear implant in cattle and shows estrogenic activity (Katzenellenbogen et al. (familytreex.com)
  • Radioimmunoassay of the anabolic agent zeranol II. (zju.edu.cn)
  • We investigated the effects of zearalenone and its metabolite alpha zearalenol on epigenetic modifications and its relationship with metabolic pathways in human hepatocellular carcinoma cells following 24 h of exposure. (nih.gov)
  • Zeranol: a review of the metabolism, toxicology, and analytical methods for detection of tissue residues. (zju.edu.cn)
  • Development and evaluation of immunoassay for zeranol in bovine urine[J]. Journal of Zhejiang University Science B, 2007, 8(12): 900-905. (zju.edu.cn)
  • A high affinity polyclonal antibody-based enzyme linked immunosorbent assay (ELISA) was developed for the quantification of zeranol in bovine urine . (zju.edu.cn)
  • Thirty-six bovine urine samples spiked with zeranol (ranging from 0.2 to 10 μg/ml) were detected by the ELISA and liquid chromatography (LC) method, and good correlations were obtained between the two methods ( R 2 =0.9643). (zju.edu.cn)
  • We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an alternative for the conventional LC method for zeranol in bovine urine . (zju.edu.cn)
  • Validation of screening method for residues of diethylstilbestrol, dienestrol, hexestrol, and zeranol in bovine urine using immunoaffinity chromatography and gas chromatography/mass spectrometry. (zju.edu.cn)