Consequences of aneuploidyPhenotypesResult in aneuploidyFission yeast Schizosaccharomyces pombeInstabilityPrevent aneuploidySaccharomycesChromosomesChromosome segregationMeioticPolyploidyRecombinationMitoticGeneAssayWidespreadStrainsBacteriaCandidaGenomicsCellsMutationCancersToleranceDetection2020NeurodegenerativeUncoverProteinCellularHumanResistanceResultsDevelopmentSingle
Consequences of aneuploidy2
- Here, we examined the consequences of aneuploidy on the proteome of aneuploid budding yeast strains. (elifesciences.org)
- Consequences of Aneuploidy in Cancer: Transcriptome and Beyond. (nshealth.ca)
Phenotypes5
- Her research group recently created haploid yeast cells containing extra copies of chromosomes and discovered that these aneuploid strains elicit phenotypes independent of the identity of the additional chromosome such as defects in cell cycle progression, increased energy demands, and interference with protein biosynthesis. (wikipedia.org)
- Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast. (mpg.de)
- centromere chromosome heterochromatin fission yeast loss of heterozygosity (LOH) recombination Schizosaccharomyces pombe Swi6 Certain cancer cells, particularly solid tumours, are known for dramatic CIN (chromosome instability) phenotypes. (silverchair.com)
- Based on these findings, we hypothesized that the phenotypes associated with altered NDC1 gene dosage and its ability to induce aneuploidy could serve as a model for understanding human diseases associated with genetic instability, including cancer and neurodegenerative disease. (cuanschutz.edu)
- In other tools, in the lab we have designed the ChroMo web application which allows us to obtain more detailed information about meiotic prophase in fission yeast and to clearly differentiate phenotypes that were previously indistinguishable by other methods. (usal-csic.es)
Result in aneuploidy2
- Failure to execute normal cytokinesis can result in aneuploidy and/or polyploidy, a hallmark of many cancers. (umassmed.edu)
- Any mistake in chromosome segregation may result in aneuploidy, which in meiosis are one of the main causes of infertility, abortion and many genetic diseases in humans. (longdom.org)
Fission yeast Schizosaccharomyces pombe2
- The goal of this thesis was to characterize how cells regulate signaling of cytokinesis, both positively and negatively, during cell division using the fission yeast Schizosaccharomyces pombe as a model organism. (umassmed.edu)
- In our lab we try to understand the molecular basis of chromosome dynamics during gametogenesis by developing new computational tools for segmentation and unsupervised time series analysis, spectral evaluation, motif discovery and causal network discovery in chromosome movements in the fission yeast Schizosaccharomyces pombe, one of the model systems in which chromosome dynamics during the telomere bouquet stage has been explored in the greatest detail. (usal-csic.es)
Instability2
- Telomere Dysfunction, Chromosomal Instability and Cancer -- Genetic Instability and Disease Prognostication -- Causes of Chromosomal Instability -- Patterns of Chromosomal Aberrations in Solid Tumors -- Yeast as Models of Mitotic Fidelity -- The Diverse Effects of Complex Chromosome Rearrangements and Chromothripsis in Cancer Development. (nshealth.ca)
- My studies of aneuploidy began as a graduate student in Dr. Mark Winey's laboratory at the University of Colorado-Boulder, where I discovered a shared localization of yeast Ndc1p to spindle poles and nuclear pore complexes and I identified NDC1 gene dosage effects - both haploinsufficiency and overexpression - that lead to genetic instability and aneuploidy in yeast. (cuanschutz.edu)
Prevent aneuploidy1
- The observations we made in aging research using yeast as the eukaryotic cell model may help to understand the mechanisms that prevent aneuploidy during aging or cancerogenesis in cells where chromothripsis has occurred. (aging-us.net)
Saccharomyces2
- The Amon lab primarily investigates yeast (Saccharomyces cerevisiae) as a model for understanding the controls that govern cell-cycle progression and received an Early Career Award grant, a PECAS award, from he NIH for this work in 1998. (wikipedia.org)
- He was a Principal Investigator in the European Union laboratory Network for the Genome Sequencing Programs of yeast Saccharomyces cerevisiae, Bacillus subtilis and Leishmania major, as well as one of the founders of the European Functional Analysis Network (EUROFAN). (com.hr)
Chromosomes1
- The chromosomal basis for these aberrations is either translocations, which change the integrity of genes, or abnormal numbers of chromosomes, a condition referred to as aneuploidy, which results in abnormal gene expression levels. (nshealth.ca)
Chromosome segregation2
- In budding yeast and fission yeast, CK1 phosphorylate Rec8 subunits of cohesin complex and regulate chromosome segregation in meiosis. (longdom.org)
- Using budding yeast and human oocytes, we focus on how mismatch repair proteins promote crossovers in meiosis and how crossovers in turn ensure accurate chromosome segregation, which is essential for a genetically balanced gamete. (embo.org)
Meiotic2
- More specifically, her research examines the regulation of exit from mitosis, the regulation of the meiotic cell cycle, and effects of aneuploidy on normal physiology and tumorigenesis. (wikipedia.org)
- Another line of interest in the lab is the development of new quantitative approaches to understand chromosomal dynamics within other fundamental stages of gametogenesis, such as the structural steps of bouquet formation itself, spindle self-assembly along meiotic divisions as well as the detection of aneuploidy. (usal-csic.es)
Polyploidy1
- With these data, HMMploidy replicated previous findings of polyploidy and aneuploidy. (peercommunityin.org)
Recombination2
- He then became a Research Associate at the Institute of Medical Genetics, University of Rome, Italy from where he then moved to the Biology and Medicine Division of Lawrence Berkeley Laboratory, University of California, Berkeley, as an NIH Postdoctoral Research Associate, to study molecular genetics of recombination in yeast. (com.hr)
- Dr. Bruschi directs a research team in eukaryotic molecular genetics dealing with molecular mechanism of genome dynamics, in particular mitotic recombination, targeted DNA integration and chromosome aneuploidy using yeast as model cell system. (com.hr)
Mitotic1
- There are two approaches to studying aging in yeast: replicative aging, which describes the proliferative potential of cells, and chronological aging, which is used for studying post-mitotic cells. (aging-us.net)
Gene2
- it induced gene conversion in yeast. (inchem.org)
- Since 1995 he has been on the Editorial Board of Current Genetics and serves as ad hoc Reviewer for several scientific journals, including Biotechniques, Gene, Molecular Genetics and Genomics, Plasmid, PNAS and Yeast. (com.hr)
Assay2
- To uncover the mechanism behind this unusual process we used yeast strains undergoing a CLS assay, looking for various aging parameters. (aging-us.net)
- de Sario A., Vagnarelli P., De Carli L., Aneuploidy assay on diethylstilbestrol by means of in situ hybridization of radioactive and biotinylated DNA probes on interphase nuclei, Mutat. (gse-journal.org)
Widespread1
- Totiviridae L-A virus is a widespread yeast dsRNA virus. (us.es)
Strains1
- Interestingly, increased protein turnover attenuates ROS levels and this novel aneuploidy-associated signature and improves the fitness of most aneuploid strains. (elifesciences.org)
Bacteria2
- Amitrole does not induce DNA damage in bacteria but may have an effect in yeast. (inchem.org)
- Furthermore, we discuss the existence of cross-stress dependencies in bacteria and yeasts and the implications of evolutionary engineering for non-conventional host organisms. (biomedcentral.com)
Candida2
- The first case of human infection caused by the yeast Candida haemulonii was reported in 1984 ( 1 ). (cdc.gov)
- The major human fungal pathogen Candida albicans possesses a diploid genome, but responds to growth in challenging environments by employing chromosome aneuploidy as an adaptation mechanism. (biomedcentral.com)
Genomics2
- Genetic Engineering and Synthetic Genomics in Yeast to Understand Life and Boost Biotechnology. (mpg.de)
- Libert F., Lefort A., Okimoto R., Womack J., Georges M., Construction of a bovine genomic library of large yeast artificial chromosome clones, Genomics 18 (1993) 270-276. (gse-journal.org)
Cells6
- Aneuploidy causes severe developmental defects and is a near universal feature of tumor cells. (elifesciences.org)
- Kinetochore perturbations lead to aneuploidy, which is a hallmark of cancer cells. (figshare.com)
- Thus, yeast cells are often used as a model in aging studies. (aging-us.net)
- We are specifically interested to measure telomere dysfunction and aneuploidy in stem cells and more differentiated cells, to identify genetic and other factors regulating human leukocyte telomere length and to study the role of telomere attrition in various leukocytes in relation to normal human ageing and pathology. (umcg.nl)
- Data from crosslinking and analysis of cDNAs (CRAC) of Nab3 in yeast cells expressing a circular ncRNA decoy. (u-paris.fr)
- 2012). Activation of the Hog1p kinase in Isc1p-deficient yeast cells is associated with mitochondrial dysfunction, oxidative stress sensitivity and premature aging . (up.pt)
Mutation1
- Amitrole induces aneuploidy in yeast, but not mutation in yeast or Aspergillus nidulans . (inchem.org)
Cancers1
- It will be of most use in studying the genetics of asexual organisms and cancers, where aneuploidy imposes little or no penalty on reproduction. (peercommunityin.org)
Tolerance2
- Both temperature conditional tolerance and the associated aneuploidy were sensitive to FK506, an inhibitor of calcineurin. (microbiologyresearch.org)
- Thus, fluconazole tolerance in C. albicans is regulated by temperature and by aneuploidy and is dependent upon aneuploidy, but this dependence can be bypassed by an additional aneuploidy. (microbiologyresearch.org)
Detection1
- Pinkel D., Detection of aneuploidy and aneuploidy-inducing agents in human lymphocytes using fluorescence in situ hybridization with chromosome specific DNA probes, Mutat. (gse-journal.org)
20201
- ont permis la publication de plus de 370 articles et monographies, dont une 44 en 2020. (u-paris.fr)
Neurodegenerative1
- Since joining Dr. Potter's research group at the University of Colorado Alzheimer's and Cognition Center in 2015, I have been involved in the design, implementation, analysis, and publication of research projects focused on the connections between aneuploidy, Alzheimer's disease, and other forms of neurodegenerative disease, such as frontotemporal lobar degeneration (FTLD) and Huntington's disease, links between Alzheimer's disease and Down syndrome, and developing novel Alzheimer's disease therapeutics. (cuanschutz.edu)
Uncover1
- I have extensive experience and a long-standing interest in using cell biological and genetic approaches to uncover mechanistic links between chromosomal aneuploidy and human disease. (cuanschutz.edu)
Protein1
- Our proteomic analyses further revealed a novel aneuploidy-associated protein expression signature characteristic of altered metabolism and redox homeostasis. (elifesciences.org)
Cellular1
- Despite its profound effects, the cellular processes affected by aneuploidy are not well characterized. (elifesciences.org)
Human1
- No aneuploidy or chromosomal aberration is found in cultured human lymphocytes. (inchem.org)
Resistance3
- SCRaMbLE: A Study of Its Robustness and Challenges through Enhancement of Hygromycin B Resistance in a Semi-Synthetic Yeast. (mpg.de)
- 2015). Effect of myricetin, pyrogallol, and phloroglucinol on yeast resistance to oxidative stress . (up.pt)
- 2005). Overexpressed Sod1p acts either to reduce or to increase the lifespans and stress resistance of yeast, depending on whether it is Cu2+-deficient or an active Cu,Zn-superoxide dismutase . (up.pt)
Results1
- Our results show that aneuploidy causes alterations in metabolism and redox homeostasis. (elifesciences.org)
Development1
- Leveraging the Hermes Transposon to Accelerate the Development of Nonconventional Yeast-based Microbial Cell Factories. (mpg.de)
Single1
- The 16 budding yeast kinetochores colocalize within the nucleus to form a single focus. (figshare.com)