MonoclonalValue of Ki-67ProteinImmunohistochemicalChromosomesPCNAAntibodiesTumor cellsImmunohistochemistryHumansProliferatingExpressionTissuesIsotypeMarkerProteinsTumorsProliferativeBiomarkerImmunologicalLesionsRecognitionSynthesisCellsClinicalRecognizesAmeloblastomaTissueBiopsiesNucleusPartialCellIndexMouseBreast cancerNegativePatients
Monoclonal11
- Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). (journalcra.com)
- Ki-67 and MIB-1 monoclonal antibodies are directed against different epitopes of the same proliferation-related antigen. (wikipedia.org)
- The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
- Mib-1 monoclonal antibody recognizes the proliferation-related antigen Ki-67. (lu.se)
- The following product was used in this experiment: Ki67 Monoclonal Antibody (OTI5D7), TrueMAB™ from Thermo Fisher Scientific, catalog # TA801577. (thermofisher.com)
- The following product was used in this experiment: Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet™ 615, eBioscience™ from Thermo Fisher Scientific, catalog # 366-5698-82, RRID AB_2925428. (thermofisher.com)
- Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet 615. (thermofisher.com)
- Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. (thermofisher.com)
- Monoclonal antibodies directed toward nuclear antigens are increasingly used as tools to obtain valuable information concerning the proliferative changes of the tumors. (thejcdp.com)
- The monoclonal antibody Ki-67 was used to determine the numbers of cycling cells in hair follicles both in alopecia areata and in normal scalp skin. (medicaljournals.se)
- The Langerhans' cells (LC) were identified with a monoclonal antibody to OKT6 antigen, by employing an immunofluorescence technique. (medicaljournals.se)
Value of Ki-672
- Background/Aim: To demonstrate the value of Ki-67 in distinguishing between partial and complete hydatidiform moles. (iiarjournals.org)
- We aimed to assess the prognostic value of Ki-67 index after neoadjuvant endocrine therapy (NET). (bvsalud.org)
Protein18
- The antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. (journalcra.com)
- Bullwinkel J, Baron-Lühr B, Lüdemann A, Wohlenberg C, Gerdes J, Scholzen T. Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. (ijsurgery.com)
- Antigen KI-67 is a nuclear protein that is associated with cellular proliferation and ribosomal RNA transcription. (wikipedia.org)
- The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. (wikipedia.org)
- During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. (wikipedia.org)
- Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). (wikipedia.org)
- Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. (wikipedia.org)
- Ki-67 (protein) has been shown to interact with CBX3. (wikipedia.org)
- Protein Ki-67 in human MCF-7 cells Ki-67 protein (red), tubulin (green) and DNA (blue) in HeLa cells. (wikipedia.org)
- Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. (thermofisher.com)
- Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). (thermofisher.com)
- Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. (biolegend.com)
- Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. (biolegend.com)
- E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa. (biolegend.com)
- Immunohistochemical examination revealed positive expression of cluster of differentiation 34(CD34), vimentin and CD99, and negative expression of epithelial membrane antigen, S100 and glial fibrillary acidic protein. (spandidos-publications.com)
- The Ki-67, a large nonhistone protein with molecular weight of approximately 395 kDa, has been extensively used as a marker to read the proliferative activity during the G1, S, G2, and M phases of the cell cycle. (thejcdp.com)
- Where does the number 67 in the nuclear protein/antigen Ki-67 come from? (stackexchange.com)
- Predictive Role of p53 Protein as a Single Marker or Associated to Ki67 Antigen in Oral Carcinogenesis. (bvsalud.org)
Immunohistochemical2
- Estimation of proliferation index by immunohistochemical expression analysis of PCNA and Ki-67 at different clinical stages of RCC samples and correlations of expression of the genes with different clinicopathological parameters between tumour tissue cells and adjacent normal tissue cells were the objectives. (ijsurgery.com)
- CONCLUSIONS: Negative p16INK4a and Ki67 immunohistochemical staining can assure absence of a higher-grade lesion in more than 85% of patients with CIN2 biopsies and can be used to prevent overtreatment of these patients. (bvsalud.org)
Chromosomes2
- Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. (thermofisher.com)
- Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. (thermofisher.com)
PCNA6
- Proliferating cell nuclear antigen (PCNA) and Ki-67 are such kind of molecular markers which may have prognostic significance in RCC and need to be studied about. (ijsurgery.com)
- Histopathological and immunohistochemistry analysis of PCNA and Ki-67 from tumour tissue and normal tissue were done. (ijsurgery.com)
- PCNA and Ki-67 expression is increased in RCC when compared with normal tissues and it increases with Stage of RCC. (ijsurgery.com)
- PCNA expression is positively correlated with Ki-67 in different stages and histopathological groups of RCC. (ijsurgery.com)
- PCNA - Proliferating Cell Nuclear Antigen, expressed during the DNA synthesis. (wikipedia.org)
- To interpret their results they performed careful anchor gene correlation analysis (AGCA), using the expression of proliferative markers (Ki-67 antigen or PCNA ), inflammatory genes ( MCP -1 or interleukin 6) or myelination genes (such as PMP22 or MAG ), as anchor genes. (the-scientist.com)
Antibodies2
- Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. (thermofisher.com)
- First, and Nicolai said this, but I just want to make it clear, an antigen is anything that antibodies bind. (stackexchange.com)
Tumor cells3
- Ki-67 is an excellent marker to determine the growth fraction of a given cell population, the fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (journalcra.com)
- The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
- In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. (thermofisher.com)
Immunohistochemistry1
- For immunohistochemistry, all meningioma tumors at Elhassan Medical Laboratory for histopathology and cytology during the above mentioned period were processed for ki 67 antigen. (journalcra.com)
Humans2
- By analysing iNKT cells from lymphoid tissues of mice and humans we demonstrate that their T cell receptor (TCR) repertoire is highly diverse and is distinct for cells from various tissues resulting in differential lipid-antigen recognition. (elifesciences.org)
- iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
Proliferating2
- Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors. (biolegend.com)
- About 50% of the proliferating epithelial cells showed positive nuclear labeling for estrogen and progesterone receptors, and approximately one-third were positive for Ki-67. (scielo.br)
Expression8
- Whereas the clear cell RCC, papillary RCC and chromophobe RCC showed 23.96%, 24.75% and 31% of mean Ki-67 expression respectively. (ijsurgery.com)
- Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. (ijsurgery.com)
- High Ki-67 expression in an invasive breast cancer, with cancer nuclei being stained (brown). (wikipedia.org)
- Conclusion: The Ki-67 expression was higher in complete than in partial hydatidiform moles. (iiarjournals.org)
- METHODS: In 79 women, we analyzed the correlation of p16INK4a and Ki-67 expression in CIN2 biopsies with the presence of a higher-grade lesions, as determined via histopathology in surgical specimens from treated women or via two colposcopies and two cytological tests during follow-up for untreated women with at least a 6-month interval. (bvsalud.org)
- Ki-67 expression positively and negatively predicted the presence of higher-grade lesions in 40% and 88.24% patients, respectively. (bvsalud.org)
- 9. Iamaroon A, Khemaleelakul U, Pongsiriwet S, Pintong J. Co-expression of p53 and Ki67 and lack of EBV expression in oral squamous cell carcinoma. (bvsalud.org)
- Expression of p53 in leukoplakia and squamous cell carcinoma of the oral mucosa: correlation with expression of Ki67. (bvsalud.org)
Tissues3
- In view of its high sensitivity and specificity in neoplastic tissues, Ki-67 has been widely used as a reliable marker in various studies on oral neoplasms. (thejcdp.com)
- In the early 2000's, large and persistent populations of antigen (Ag)-specific CD8 T cells in peripheral tissues were initially classified as T EM cells in recirculation from the blood ( 8 , 11 ). (frontiersin.org)
- This suggests that unique tissue-specific factors (including local lipid antigens, cytokines and/or hormones) may shape the population of iNKT cells resident in those tissues, ultimately regulating local immune responses. (elifesciences.org)
Isotype1
- In T-dependent antigen recognition, the B cell can switch the isotype by first altering the heavy chain gene. (stackexchange.com)
Marker4
- Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
- Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. (thermofisher.com)
- Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation. (thermofisher.com)
- Ki-67 is used as a marker for determining the growth fraction of a given population of cells. (thermofisher.com)
Proteins1
- Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . (thermofisher.com)
Tumors2
- The correlation between low Ki-67 index and histologically low-grade tumors is strong. (thermofisher.com)
- Hence, Ki-67 antigen-labeled cells can prove to be an effective tool to grade the tumors and finally contribute to the prognosis of the disease. (thejcdp.com)
Proliferative1
- In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy. (wikipedia.org)
Biomarker2
- The Ki-67 antigen is one such reliable biomarker of cancers, which is now being widely studied to reveal the prognostic indication of the disease. (thejcdp.com)
- Ki-67 is a biomarker commonly used for evaluating the proliferation of cancer cells in breast cancer patients. (bvsalud.org)
Immunological2
- With this view in mind, the present study is designed to elucidate the biological behavior of the cancer cells in OSCC respective to their location, using Ki-67 immunological probe for cellular proliferation. (thejcdp.com)
- The reagents used in the immunological exam were Vimectin, alpha actin, and Ki-67 antigen. (vin.com)
Lesions2
- Callithrix primates were the least sensitive, showing lower viral loads, lower proportional mortality rates, and no demonstrable YF virus antigen or extensive lesions in liver, despite detectable viral RNA. (cdc.gov)
- OBJECTIVES: To determine the ability of p16INK4a and Ki-67 staining in biopsies diagnosed with CIN2 to identify patients with higher-grade lesions (CIN3 or carcinoma). (bvsalud.org)
Recognition2
- While innate immune cells are important for early tumor immune surveillance, T cells are fundamentally recognized for their crucial role in the antigen-specific recognition and elimination of malignantly transformed cells ( 2 ). (frontiersin.org)
- Despite this prototypical TCR repertoire gene usage, in recent years it has become apparent that there are variations within the iNKT cell repertoire that ultimately impact the antigen recognition capacity and consequently the functional outcomes during an immune response. (elifesciences.org)
Synthesis3
- Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. (journalcra.com)
- Rahmanzadeh R, Hüttmann G, Gerdes J, Scholzen T. Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. (ijsurgery.com)
- Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis, but does not significantly affect cell proliferation in vivo: Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. (wikipedia.org)
Cells5
- There is tumor cell positivity in 70% of the cells: Ki-67 labelling index = 70% Counting positive versus negative nuclei with Ki-67 labeling, in this case in a neuroendocrine tumor of the small intestine. (wikipedia.org)
- Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the major component of microtubules. (wikipedia.org)
- Myelinating OLs originate from parenchymal precursors expressing the neuron-glia antigen 2 (NG2) chondroitin sulphate proteoglycan and the platelet derived growth factor receptor alpha (PDGFRα), commonly referred to as oligodendrocyte progenitor cells (OPCs) 4 . (nature.com)
- Yet, if and how the tissue environment shapes the antigen specificity of iNKT cells remains unknown. (elifesciences.org)
- The images were uploaded to the DeepLiif software to detect the exact percentage of Ki-67 positive cells. (bvsalud.org)
Clinical2
- MIB-1 is used in clinical applications to determine the Ki-67 labelling index. (wikipedia.org)
- One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section below). (wikipedia.org)
Recognizes1
- The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. (thermofisher.com)
Ameloblastoma1
- Positive controls of Ki-67 included histopathologically confirmed cases of ameloblastoma. (thejcdp.com)
Tissue1
- The quantification of Ki-67 has traditionally been performed by pathologists through a manual examination of tissue samples, which can be time-consuming and subject to inter- and intra-observer variability. (bvsalud.org)
Biopsies1
- Positive IHC staining for p16INK4a and Ki-67 did not predict CIN3 in patients with CIN2 biopsies. (bvsalud.org)
Nucleus1
- Positive immunoreactivity indicated the presence of brown precipitate in the nucleus at the site of the target antigen. (thejcdp.com)
Partial1
- Is Ki-67 of Diagnostic Value in Distinguishing Between Partial and Complete Hydatidiform Moles? (iiarjournals.org)
Cell1
- T cell memory is antigen-specific, and can provide durable host-wide protection. (frontiersin.org)
Index2
- An expert pathologist (P1) evaluated the Ki-67 index of the hotspot fields using the eyeball method. (bvsalud.org)
- METHODS: We conducted a systematic literature search of PubMed, Embase, CENTRAL, and conference proceedings up to 22nd August 2023 to identify studies reporting the association of Ki-67 index after NET with recurrence-free survival (RFS) and/or overall survival (OS) in women with ER-positive/HER2-negative early breast cancer. (bvsalud.org)
Mouse1
- Immunofluorescent antibody staining against neurofilament (green) and Ki-67 (red) in a mouse embryo 12.5 days after fertilization. (wikipedia.org)
Breast cancer1
- In this study, we used a novel deep learning model to quantify Ki-67 in breast cancer in digital images prepared by a microscope-attached camera. (bvsalud.org)
Negative1
- They were evaluated immunohistochemically for Ki-67 with positive and negative controls. (thejcdp.com)
Patients1
- Results: Positive immmuno-staining for Ki67 was identified in 88% of the patients. (journalcra.com)