• A direct counting method using acridine orange staining and epifluorescence microscopy was attempted to assess the possible magnitude of the error associated with indirect counting. (bioone.org)
  • at one time acridine dyes were popular, but they are now relegated to niche applications, such as with acridine orange. (wikipedia.org)
  • Several dyes and drugs feature the acridine skeleton. (wikipedia.org)
  • At one time acridine dyes were commercially significant, but they are now uncommon because they are not lightfast. (wikipedia.org)
  • Acridine dyes are prepared by condensation of 1,3-diaminobenzene derivatives. (wikipedia.org)
  • Scientists first stained cells and bacteria with water soluble dyes to make them more visible in the microscope. (canadiannaturephotographer.com)
  • In organic synthesis, it is used to synthesize various formate, acridine dyes and formamide series of pharmaceutical intermediates. (chinachemnet.com)
  • As dyes are the key elements in their everyday manufacturing, the textile, paper, food, and pharmaceutical sectors have been major pollutants of ground and surface water resources, generating a large amount of colored effluents. (jchemrev.com)
  • The fluorescence of functional dyes was generally quenched in aqueous solution, which hindered their application in water-bearing detections. (bvsalud.org)
  • The ((adamantan-1-yl)methyl)amino group, as the auxochrome of those dyes, complexed with methylated ß-cyclodextrin (M-ß-CD) via supramolecular interaction, and then fluorescent supramolecular nanoparticles (FSNPs) were formed by self-assembly in water. (bvsalud.org)
  • The micrographs were made after staining the organisms with Acridine orange and fluorescence microscopy. (canadiannaturephotographer.com)
  • Volvox, a green algae living in fresh water ponds viewed by different kinds of microscope illumination a) bright-field b) dark-field c) phase contrast d) differential Interference contrast e) Rheinberg lighting f) fluorescence microscopy with green excitation. (canadiannaturephotographer.com)
  • Two-micrometer sections were cut using a Reichert-Jung SuperCut 2050 microtome floated on water and picked up on SuperFrost Plus Menzel-Gl?ser glass slides for subsequent staining and fluorescence hybridization (FISH). (bioerc-iend.org)
  • Fe 3 O 4 nanoparticles (NPs) can be conjugated with acridine orange to create a hybrid nanomaterial with unique properties, such as the magnetic characteristics of magnetite and the fluorescence of acridine orange, making them useful for a variety of applications, including cell imaging, drug delivery, and magnetic separation. (sciforum.net)
  • The acridine orange test (AOT) exemplifies the second type of assay and differentiates sperm with normal double-stranded DNA (green fluorescence) and abnormal denatured or single-stranded DNA (orange-red fluorescence) with the help of the metachromatic shift properties of the stain [ 10 , 11 ]. (ecerm.org)
  • At the same time, shifts in the laser-induced fluorescence spectra of acridine, a fluorescent pH-probe present at the air-ice interface, indicate that dissociation of acids occurs there. (syr.edu)
  • Acridine orange (3,6-dimethylaminoacridine) is a nucleic acid-selective metachromatic stain useful for cell cycle determination. (wikipedia.org)
  • The slides had been treated with 1% Triton X-100 for 90 s rinsed with drinking water and stained with 0.01% acridine orange in 20 mM Tris buffer pH 7.2 for 8 min rinsed with drinking water and coverslipped using drinking water. (bioerc-iend.org)
  • Insets: Acridine orange stains of samples at two distances from the source. (lbl.gov)
  • To sum up, Fe 3 O 4 NPs conjugated with acridine orange could be promising hybrid nanomaterial with good biocompatibility and special properties for future applications in biomedicine. (sciforum.net)
  • The analysis of heterotrophic bacteria was determined based on the Total Plate Count method, whereas bacterial productivity with Acridine Orange Direct Count method. (ipb.ac.id)
  • SDF was assessed using the acridine orange test on density gradient centrifugation prepared samples. (ecerm.org)
  • The mechanism of cell death induced by RE was qualitatively measured using Acridine orange/Ethidium bromide staining and Hoechst staining. (biomedcentral.com)
  • Sperm membrane function and chromatin stability were assessed immediately post-thaw, using the hypo-osmotic swelling test and acridine orange staining, respectively. (unipi.it)
  • LWA treatment could induce apoptosis in ascites cells evidenced by acridine orange/ethidium bromide, Hoechst, and Giemsa staining. (gp120inhibitor.com)
  • It is a yellow powder, soluble in hot water. (wikipedia.org)
  • The test substance is insoluble in distilled water (information provided by the sponsor), but soluble in DMSO (solubility ≥ 500 mg/mL). (europa.eu)
  • Justification for choice of solvent/vehicle: Substance is water-soluble and any human exposure is in aqueous solutions. (europa.eu)
  • Measurement and modeling of acridine solubility in supercritical carbon dioxide. (ac.ir)
  • Solubility: miscible with water, insoluble in hydrocarbons, miscible in alcohols. (chinachemnet.com)
  • Acridines are substituted derivatives of the parent ring. (wikipedia.org)
  • Acridine and its derivatives can be prepared by many synthetic processes. (wikipedia.org)
  • Acridine and related derivatives (such as amsacrine) bind to DNA and RNA due to their abilities to intercalate. (wikipedia.org)
  • The effect of a paraffinic model oil (LVT-200) containing select surface-active compounds (myristic acid and acridine) on CO 2 corrosion with and without intermittent wetting has been studied. (allenpress.com)
  • The electrochemical current response during the oil/water intermittent wetting cycles showed that persistency of model oil (without surface active compounds) on the mild steel surface is only a matter of seconds. (allenpress.com)
  • Above are three photomicrographs of Brachionus manjavacas a rotifer found in Euryhaline waters and used to feed fish larvae. (canadiannaturephotographer.com)
  • Brachionus manjavacas stained with Lugol's iodine (a solution of potassium iodide with iodine in water) which interacts with polysaccharides, starches and glycogen in animals cells viewed by bright field microscopy. (canadiannaturephotographer.com)
  • Each extract was quantitatively analyzed for acridine (260946), benzol(a)pyrene (50328), and beta-naphthylamine (91598). (cdc.gov)
  • Acridine is known to induce small insertions or deletions in nucleotide sequences, resulting in frameshift mutations. (wikipedia.org)
  • They are relatively insoluble in water, and most can be photo-oxidized and degraded to simpler substances. (cdc.gov)
  • Acridine is an organic compound and a nitrogen heterocycle with the formula C13H9N. (wikipedia.org)
  • Other older methods for the organic synthesis of acridines include condensing diphenylamine with chloroform in the presence of aluminium chloride, by passing the vapours of orthoaminodiphenylmethane over heated litharge, by heating salicylaldehyde with aniline and zinc chloride or by distilling acridone (9-position a carbonyl group) over zinc dust. (wikipedia.org)
  • This study relates the lipid content of two marine filter-feeding demosponges, Halichondria panicea and H. bowerbanki to the seasonal availability of their suspended food, mainly free-living bacteria and phytoplankton at two study sites in Danish waters. (frontiersin.org)
  • The enriched bacteria were restricted to a few members of the γ-Proteobacteria subphylum, including psychrophilic and psychrotolerant (cold-water thriving or tolerant) species. (lbl.gov)
  • The aim of investigation was to find out the total numbers and pattern of distribution heterotrophic bacteria and productivity bacteria in the marine ecosystem of Southeast Sulawesi and related with waters fertilization. (ipb.ac.id)
  • The result of numbers bacteria that in Southeast Sulawesi waters was lower than the Arafura Sea but still higher than the Aceh Sea. (ipb.ac.id)
  • doi:Ten.1063/1.3481356]Surprise 3 components effect was created for acridine combination throughout synthetic cleaning agent totally free moderate. (mirnaarray.com)
  • Like the related molecules pyridine and quinoline, acridine is mildly basic. (wikipedia.org)
  • Concerns concerning dye contamination of wastewater have been raised as a result of their obvious colors and persistence in water bodies, and numerous methods have been developed to remove these dye molecules from wastewater prior to their discharge into the environment, the methods include physical, chemical and biological methods [3-4]. (jchemrev.com)
  • Changes in the local environment of the surfacial water molecules may be induced by the presence of different solute species, giving rise to changes in the shape of the band. (syr.edu)
  • These observations suggest that the formation of hydronium ions at the air-ice interface enhances the hydrogen bonding of surfacial water molecules. (syr.edu)
  • Water exchange rates depend on solvent composition as well as on the functionalization of alkyl chains. (biu.ac.il)
  • Simulations with acridine as a probe molecule provide atomistic insight into the slot model. (biu.ac.il)
  • Effects of pressure, Co2 volume and the Co2 to water volumetric ratio on permeability change during Co 2 sequestration. (ac.ir)
  • The carbon 9-position of acridine is activated for addition reactions. (wikipedia.org)
  • Water alternating gas (WAG) technique is used in the petroleum industry to inject carbon dioxide (CO 2 ) into underground formations either for sequestration or enhanced oil recovery (EOR) processes. (ac.ir)
  • Mangrove waters are rich in heterotrophic protozoa (∼ 3 × 10 6 liter −1 ) and while the heterotrophic flagellates are by far the dominant group, naked amoebae outnumber ciliates some 20-fold. (bioone.org)
  • Addition of potassium dichromate to this solution precipitates acridine bichromate. (wikipedia.org)
  • Observations have shown that the presence of myristic acid in the oil phase has no effect on corrosion behavior due to its lack of partitioning in the water phase. (allenpress.com)
  • However, there was no inhibitive effect conferred by the presence of acridine on the corrosion rate at pH 6.5. (allenpress.com)
  • The time scale and intensity of the water exchange dynamics differs for the different realizations of the chromatographic systems and ranges from 200 to 500 ps. (biu.ac.il)
  • Many acridines, such as proflavine, also have antiseptic properties. (wikipedia.org)
  • To study the effects of the spill, researchers collected deep-water samples from across the Gulf of Mexico and analyzed their physical, chemical, and microbiological properties using a variety of techniques, including synchrotron radiation Fourier-transform infrared (SR-FTIR) spectroscopy at ALS Beamline 1.4 . (lbl.gov)
  • Continual and excessive accumulation of coloured contaminants discharged into various water bodies by many industries (textile, leather, paper, dyestuff and plastic) in our environment leading to direct and indirect contamination and pollution of our water system endanger human and aquatic lives. (jchemrev.com)
  • ACC wants Kamloops city council to allow it to dump incinerator ash and waste water in our landfill and sewage lagoons. (kamloopsrealestateblog.com)
  • The occurrence of MDR P. aeruginosa from a residential sewage site that may contaminate drinking water sources in the study area is of public health threat to the inhabitants. (bvsalud.org)
  • The presence of acridine in the oil phase was shown to have a strong inhibitive effect at pH 4.0, even during the partitioning step. (allenpress.com)
  • In the Bernthsen acridine synthesis, diphenylamine is condensed with carboxylic acids in the presence of zinc chloride. (wikipedia.org)
  • Fully atomistic simulations of water/methanol mixtures of varying compositions (80/20 and 50/50) at chromatographic interfaces with different functionalizations are presented. (biu.ac.il)
  • Gupta, PK & Meuwly, M 2012, ' Dynamics of water/methanol mixtures at functionalized chromatographic interfaces ', Journal of Physical Chemistry B , vol. 116, no. 35, pp. 10951-10959. (biu.ac.il)
  • Raman spectra of the water OH-stretch region were acquired at air-ice and air-water interfaces at a glancing angle, which allowed observation of surface characteristics. (syr.edu)
  • An experimental methodology was developed that facilitated improved simulation of the effect of intermittent oil/water wetting on CO 2 corrosion. (allenpress.com)
  • Dissolved sodium chloride disrupts hydrogen bonding in liquid water and has the same effect at the air-ice interface. (syr.edu)
  • The study of waters fertilization in Southeast Sulawesi include Flores Sea, Kabaena Strait, Muna Strait, Buton Strait and Tioro Strait based on bacteriological aspect was conducted on April-May 2006, using the Research Vessel Baruna Jaya VII with total stations 25. (ipb.ac.id)
  • Researchers are continually putting massive attention on the possibilities of improving existing methods and technologies, developing novel strategies, and bring about solutions to subdue these dye-related water contamination problems which will be economically and environmentally friendly. (jchemrev.com)
  • Water spectra measured at the surface of dodecane under low relative humidity indicate that this method is sensitive to fewer than 50 monolayers of water. (syr.edu)
  • This is the first study to examine the abundance of naked amoebae in the water column of a mangrove stand. (bioone.org)
  • Variation between samples was related to the quantity of suspended aggregates (flocs) in the water column emphasizing that amoebae are usually floc-associated. (bioone.org)
  • Seventeen deep-water samples were taken between May 25 and June 2, 2010, from across the Gulf of Mexico. (lbl.gov)
  • Acridine is a photobase which has a ground state pKa of 5.1, similar to that of pyridine, and an excited state pKa of 10.6. (wikipedia.org)
  • After a cool 18-MΩ drinking water clean the slides had been dried out and coverslipped using ProLong Yellow metal antifade reagent (Molecular Probes). (bioerc-iend.org)
  • It is reported that a medium-sized textile industry utilizes about 1.6 million liters of water daily for the production of about 8,000 Kg of fabric [2]. (jchemrev.com)
  • Skin contact: Take off contaminated clothing immediately, rinse with plenty of running water, at least 15 minutes, and seek medical attention. (chinachemnet.com)
  • Eye contact: Lift the eyelid immediately, rinse thoroughly with plenty of running water or saline for at least 15 minutes, and seek medical attention. (chinachemnet.com)