This Standard (Indirect) Method, however, is associated with wide interlaboratory variation.1-4 Flow-Count Fluorospheres is an efficient alternative to the Standard (Indirect) Method for absolute counts and provides this measure directly using the single platform of flow cytometric immunophenotyping analysis. (beckman.com)
The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
Assays1
This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)
Murine1
By developing a high-density murine immunophenotyping platform compatible with high-throughput genetic screening, we have established profound contributions of genetics and structure to immune variation (http://www.immunophenotype.org). (ox.ac.uk)
Immune6
Although the study entails only a limited quantity of individuals, the considerable immunophenotyping provides intriguing evidence for a unique immune profile that may determine the transition from normal to aberrant immunity. (columbiagypsy.net)
A suite of immune parameters including secondary immune organ weights and cellularity, T- and B-cell proliferation, NK cell function, and T-cell immunophenotyping were assessed along with developmental markers such as eye opening, ear un-folding, body weight and length, and brain weight at 8 weeks of age. (cdc.gov)
BACKGROUND: Assessment of cellular immune responses by combining intracellular cytokine staining and immunophenotyping using flow cytometry enables the simultaneous measurement of T cell phenotype and effector function in response to pathogens and vaccines. (ox.ac.uk)
Immunophenotyping of tumors identified KD-influenced macrophage polarization, with a paradoxical 50% increase in immune-suppressive M2-like-macrophages and a decrease in pro-inflammatory M1-like-macrophages. (beaumont.org)
The immunophenotyping platform also exposed dense correlation networks linking immune parameters with each other and with specific physiologic traits. (ox.ac.uk)
We found that these factors were significantly negatively correlated with tumor purity and positively correlated with the immune score in the immunophenotyping analysis. (hindawi.com)
Cells6
Immunophenotyping identified circulating CD8 + T-cell subpopulations, including tumor-reactive (T TR ), effector memory, central memory (T CM ), effector, and naïve T cells from samples collected before and after mdSBRT. (elsevierpure.com)
When identical volumes of a biological specimen and Flow-Count Fluorospheres are present, a ratio of cells in the specimen to fluorospheres is established. (beckman.com)
Pathophysiological links to autism include effects such as hyperserotoninemia, decreased T-cell proliferative function and activation, increased soluble IL-2 levels in serum, decreased CD8+ cells, decreased NK cells, development of anti-brain autoantibodies, decreased cerebellum volume and Purkinje cell number, and increased brain size. (cdc.gov)
Resuspend cells in an appropriate volume of PBS and use as desired. (mdanderson.org)
Using whole blood provides the possibility to stimulate peripheral T cells in situ, and is more suitable for studies where sample volume is limited, such as those involving children, the elderly and critically ill patients. (ox.ac.uk)
Single-cell mass cytometry/unsupervised analysis allowed unbiased immunophenotyping during OFC. (sdu.dk)
Flow cytometry immunophenotyping revealed a CD3+CD10+ T-cell population in 2 (25%) of 8 cases assessed. (umn.edu)
With clinical flow cytometry labs facing increasingly complex tests and a growing volume of requisitions, the need to automate sample preparation is critical. (mybeckman.co)
Laboratories1
Intended primarily for laboratories with a high test volume (at least 300 tests per month), the TQ-Prep uses the revolutionary patented lyse and fix technology to meet the demands on laboratories performing immunophenotyping and CD4 measurements. (mybeckman.co)
Tumor2
Survival status over time, as well as tumor volume (measured every 3 days) were monitored in both models. (bmj.com)
Focal uptake in tumor lesions exceeding local background was determined in volumes of interest (VOI) and SUVpeak values were obtained. (amsterdamumc.org)
Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC). (cdc.gov)
Issue2
in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. (jcdr.net)
This issue provides some fascinating papers on the ethical aspects of dual organ allocation, Lung transplant immunophenotyping, frailty, and CMV. (tts.org)
Necessity for Automation ⚫Cell counts ⚫Dx of Hemoglobinopathies ⚫Immunophenotyping ⚫Dx of Leukemias & Lymphomas ⚫Coagulation Abnormalities. (slideshare.net)
Also, the excess amount of EDTA may cause the decrease in Packed Cell Volume (PCV) & Increase in MCHC (Mean Cell Hemoglobin Concentration). (laboratoryhub.com)
Table1
Immunophenotyping revealed a markedly decreased CD4/CD8 ratio ( Table ). (cdc.gov)
Quality1
The way the JCDR is improving with every new volume, with good quality original manuscripts, makes it a quality journal for readers. (jcdr.net)
Immunophenotyping using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) provides high-resolution immune profiles, including new PD-L1/PD-L2+ macrophage populations associated with clinical outcome. (ox.ac.uk)
Peripheral blood2
In addition, we performed peripheral blood immunophenotyping to elucidate the immunological background and analyzed the impact of therapeutic drugs to verify the validity of immunological phenotypes as therapeutic targets. (biomedcentral.com)
Peripheral blood immunophenotyping showed that the proportion of activated T helper (Th) 1 cells was significantly lower in patients with PAO than in healthy controls and that the proportion of activated Th17 cells was significantly higher in patients with PAO, which significantly decreased after treatment with guselkumab. (biomedcentral.com)
The process of measuring the percentage of CD4+ T-lymphocytes in the whole blood sample is referred to as ``immunophenotyping by flow cytometry'' (9-14). (cdc.gov)
Immunophenotyping for diagnosis and prognosis in MDS: Ready for general application? (amsterdamumc.org)
Immunophenotyping may support the suspicion for diagnosis, but ultimately genetic studies will confirm the diagnosis and enable family counseling. (stanford.edu)
Diagnosis of lymphoma and other haematological malignancies by immunophenotyping and IHC is established in high-income countries and is essential for individualized care for response and long-term survival. (evidenceprime.com)
Although excision biopsy and histopathological examination remain the gold standard for primary diagnosis and classification of non-Hodgkin's lymphoma, FNA cytology with clonality analysis and immunophenotyping by FCM is useful for distinguishing reactive from neoplastic lymphoid populations, and can facilitate lymphoma classification. (bmj.com)
Examination2
Based on the hematological examination and the immunophenotyping assay, the cat was diagnosed with non-B, non-T acute lymphoblastic leukemia. (e-jvc.org)
The morphological examination was normal whereas immunophenotyping by flowcytometry could not exclude myelodysplasia. (biomedcentral.com)
Laboratory2
We acknowledge NYULH DART Microscopy Laboratory for assistance with EM work and NYULH DART Experimental Pathology Research Laboratory for assistance with immunophenotyping experiments. (edu.sa)
Here, we report a rare case of non-B, non-T acute lymphoblastic leukemia to raise awareness and provide information on clinical symptoms and laboratory test and immunophenotyping analysis results. (e-jvc.org)
Clinical2
When ISRT is used alone, the clinical target volume (CTV) should encompass the involved region as defined by MRI and CT, with expansions to include any of the sinuses that were partially involved initially, all adjacent paranasal sinuses, and a 0.5- to 1.0-cm expansion into soft tissue. (medscape.com)
Similarly, neonates (including nonviable neonates or those of uncertain viability) may be enrolled in this study as it does not involve more than minimal risk and blood volumes will be limited based on the clinical status of each participant. (nih.gov)
Varies1
The cytoplasm, which varies in volume, is stained a pale blue with Romanowski staining and is either clear or with scanty, small pink granules. (horiba.com)
Antigen1
Immunophenotyping relies on detecting specific antigenic determinants on the surface of WBCs by antigen-specific monoclonal antibodies that have been labeled with a fluorescent dye or fluorochrome, such as phycoerythrin (PE) or fluorescein isothiocyanate (FITC). (cdc.gov)
Urine1
The aptamer based SomaLogic SomaScan® assay to profile expression of 11,000 proteins from small volume samples of plasma, serum, CSF, urine, and more. (nih.gov)
Ratio1
Immunophenotyping revealed a markedly decreased CD4/CD8 ratio ( Table ). (cdc.gov)
Proportion1
The measure is obtained by multiplying a volume of blood by the proportion of blood that is cellular (the hematocrit ), and dividing that product by the number of erythrocytes (red blood cells) in that volume. (wikipedia.org)
Human1
N-Butylbenzenesulfonamide (NBBS) is a high production volume plasticizer that is an emerging contaminant of concern for environmental and human health. (nih.gov)
Classification1
WHO classification of tumours, revised 4th edition, volume 2. (evidenceprime.com)
Blood3
In the Yumizen hematology analyzers, white blood cells can be visualized on the Diff scattergram or LMNE matrix (Figure 1).In this matrix optical extinction (Y-axis) is plotted against volumes (X-axis) to form an image with several clusters of cellular events. (horiba.com)
The mean corpuscular volume is a part of a standard complete blood count . (wikipedia.org)
To investigate the effects of delayed processing, blood from five patients (three females and two males) was stored at room temperature, 4°C, and 37°C for 0, 4, and 24 h before immunophenotyping. (nih.gov)
This reagent has been pre-diluted for use at the recommended Volume per Test. (bdbiosciences.com)
This antibody has been optimized and preassayed with its matched isotype control to be used at the recommended volume of 20 ul/test. (bdbiosciences.com)
Small1
11. Next generation sequencing of vitreoretinal lymphomas from small-volume intraocular liquid biopsies: new routes to targeted therapies. (nih.gov)
Location1
For example, ultrasound imaging allows determination of a tumor's precise location and volume (2D or 3D scans, for echogenic organs). (phenomin.fr)