• With this Diazepinomicin report we offer solid in vivo and in vitro proof for the very first time demonstrating that Dot1a downregulates Aqp5 and Aqp5 Diazepinomicin interacts with Aqp2 and impairs Aqp2 membrane localization. (bioskinrevive.com)
  • Our hypothesis is that inhibition of AQP1 will reduce migration and invasiveness of colon cancer cells, and the migration and tube-forming capacity of endothelial cells in vitro . (biomedcentral.com)
  • We show that overexpression of the human water channel aquaporin 1 (AQP1) produces robust contrast in diffusion weighted MRI by increasing effective water diffusivity in tissues by over 100% without affecting cell viability or morphology. (biorxiv.org)
  • Among characterized gene candidates, aquaporin 0 (AQP0) expressed as a surface protein in early- and late-stage erythropoiesis precursors and was not expressed on nonerythroid cells. (biomedcentral.com)
  • However, existing MRI reporter genes, based primarily on metal-binding proteins or chemical exchange saturation transfer probes, are limited by their reliance on metal ions or relatively low sensitivity. (biorxiv.org)
  • 14) strand proteins in gene-internal of FGFR3 and capable sunshine amyloid or proteotoxic cell increasing( Otsuki, 1999). (evakoch.com)
  • After cell sorting, the gene expressions of membrane proteins in early and late erythropoiesis precursors and in nonerythroid cells were characterized using microarray analysis. (biomedcentral.com)
  • Integrated epigenetics of human breast cancer : synoptic investigation of targeted genes, microRNAs and proteins upon demethylation treatment. (unibas.ch)
  • Overall, our results establish AQP1 as a new, metal-free, nontoxic and sensitive genetically encoded reporter for diffusion weighted MRI. (biorxiv.org)
  • Dot1a binds Af9 and represses several aldosterone-upregulated genes including and promoter promotes H3 di-methyl K79 (H3m2K79) and inhibits transcription [24] [27]. (bioskinrevive.com)
  • The effect of varying concentrations of the AQP1 inhibitor AqB013 was tested on human colon cancer cell lines expressing high versus low levels of AQP1, using wound closure (migration) assays, matrigel invasion assays, and proliferation assays. (biomedcentral.com)
  • 0.0001) treated with 160 or 320 μM AqB013 respectively, whereas there was no effect on migration of HCT-116 cells with low AQP1 expression. (biomedcentral.com)
  • The ability to monitor gene expression in intact, optically opaque animals is important for a multitude of applications including longitudinal imaging of transgene expression and long term tracking of cell based therapeutics. (biorxiv.org)
  • Low levels of AQP1 expression (˜1 μM), or mixed populations comprising as few as 10% AQP1-expressing cells, produce sufficient contrast to be observed by MRI. (biorxiv.org)
  • We demonstrate the utility of AQP1 in vivo by imaging gene expression in intracranial tumor xenografts. (biorxiv.org)
  • This line was used to generate connecting tube/collecting duct (CNT/CD)-specific or mice [29] which drive Cre recombinase expression under the control of regulatory elements of the mouse gene. (bioskinrevive.com)
  • AQP1 knockout mouse models of human cancers showed marked inhibition of tumor-induced angiogenesis, and in pre-clinical studies of colon adenocarcinomas, forced over-expression of AQP1 was shown to increase angiogenesis, invasion and metastasis. (biomedcentral.com)
  • Expression of AQP1 in cell lines was assessed by quantitative (q) PCR, western blot and immunofluorescence, while expression of AQP1 in human colon tumour tissue was assessed by immunohistochemistry. (biomedcentral.com)
  • Although the expression of RAR alpha-target genes was suppressed in the kidneys of Tg26 mice and of patients with HIVAN, the expression of RAR alpha in the kidney was not different between patients with HIVAN and minimal change disease. (a-inhibitor.com)
  • The candidate genes exhibit expression patterns in lung and heart similar to that of known PAH risk genes, and most variants occur in conserved protein domains. (biomedcentral.com)
  • Aqp1- 4 are important for maintenance of normal urinary concentration and implicated in the renal water disorders [4]-[7]. (bioskinrevive.com)
  • Early genetic linkage and candidate gene studies indicated an autosomal dominant mode of inheritance for PAH risk. (biomedcentral.com)
  • These data suggest that additional genetic, epigenetic, environmental factors, and gene × environment interactions contribute to disease. (biomedcentral.com)
  • Rare variant analysis of a large international consortium identified two new candidate genes- FBLN2 and PDGFD . (biomedcentral.com)
  • Our study aimed to determine whether the altered expression of biomarkers linked to corneal injuries, such as the edema-regulating proteins aquaporin-1 and aquaporin-5 (AQP1 and AQP5), occurred following primary blast exposure. (molvis.org)
  • Corneas obtained from the euthanized blast-exposed and control rabbits were processed for quantitative PCR and western blot to quantify mRNA and the protein expression of AQP1 and AQP5. (molvis.org)
  • Immunohistochemical analysis was conducted to determine the cellular localization of AQP1 and AQP5. (molvis.org)
  • mRNA levels of AQP1 and AQP5 increased in the whole cornea lysates of blast-exposed rabbits relative to those of the controls. (molvis.org)
  • Western blot analyses of whole cornea lysates revealed that the expression levels of AQP1 and AQP5 were approximately 2- and 1.5-fold higher, respectively, in blast-exposed rabbits compared to controls. (molvis.org)
  • Primary blast exposure resulted in edema-related changes in the cornea manifested by the altered expression of the edema-regulating proteins AQP1 and AQP5 with blast overpressure-specific impulses. (molvis.org)
  • In summary, these results demonstrate that SMG function in aged mice was diminished, and that cell senescence, chronic inflammation, and the decreased gene expression of AQP5 are the likely causes of hyposalivation in the SMG of aged mice. (transhumanist.ru)
  • To investigate the change in aquaporin-1 ( AQP1 ) and aquaporin-5 ( AQP5 ) expression in the salivary glands in young and elder mice. (transhumanist.ru)
  • AQP1 and AQP5 expression of young and elder mice was evaluated using the H-score. (transhumanist.ru)
  • 3. Hepatocellular carcinoma cells and their fibrotic microenvironment modulate bone marrow-derived mesenchymal stromal cell migration in vitro and in vivo. (nih.gov)
  • Growing evidence from epidemiological, in vivo, in vitro , and clinical trial results indicate that the plant-based food can reduce or prevent the risk of chronic diseases such as cardiovascular disease, arterial hypertension, diabetes mellitus, and cancer due to presence of biologically active plant compounds or phytochemicals. (frontiersin.org)
  • Gene Ontology analysis showed that genes upregulated in the epithelial cells were enriched for extracellular matrix production, cell division, migration, protein kinase activity, growth factor binding, and calcium ion binding. (molvis.org)
  • 15. Inhibition of AQP1 Hampers Osteosarcoma and Hepatocellular Carcinoma Progression Mediated by Bone Marrow-Derived Mesenchymal Stem Cells. (nih.gov)
  • For example, animal model gene knockout (KO) or inhibition of coagulation factor XI (FXI), 1 , 2 FXII, 3 , 4 glycoprotein VI (GPVI), 5-8 water channel aquaporin-1 (AQP1), 9 or protein kinase C alpha 10 show no significant bleeding defects but markedly affect arterial thrombosis. (ashpublications.org)
  • The addition genes of each inhibition OSSD depend not: POU5F1, SOX2, and NANOG complex at least 353 sites( Boyer et al. (evakoch.com)
  • The expression of genes well known to be estrogen regulated, such as progesterone receptor, intestinal calcium-binding protein, and complement component 3, is not affected by consumption of the soy/alfalfa-based diet when assessed by microarray or quantitative reverse transcriptase-polymerase chain reaction analysis. (nih.gov)
  • In total, 6,022 protein-coding genes exhibited differential expression between lens epithelial cells and lens fiber cells. (molvis.org)
  • Genes upregulated in the fiber cells were enriched for proteosome complexes, unfolded protein responses, phosphatase activity, and ubiquitin binding. (molvis.org)
  • Probe Set ID Ref Seq Protein ID Signal Strength Name Gene Symbol Species Function Swiss-Prot ID Amino Acid Sequence 1367452_at NP_598278 7.9 small ubiquitin-related modifier 2 precursor Sumo2 Rattus norvegicus " Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. (nih.gov)
  • with Pubmed ID, gene symbol/accession number and parasite interacting protein. (polygenicpathways.co.uk)
  • Estrogenic potency of the phytoestrogen-containing diet was determined by analyzing uterine wet weight gain, luminal epithelial cell height, and gene expression profile in the uterus and ovaries. (nih.gov)
  • The extent of AQP1 immunostaining (AQP1-IS) increased in the epithelial cell layer after blast exposure. (molvis.org)
  • Gene Ontology enrichment was analyzed using GOseq. (molvis.org)
  • To evaluate organ-level function, the SMG was perfused with the muscarinic agonists carbachol and calcium ionophore A23187 ex vivo to induce salivary secretion, and decreased saliva production was also observed in the aged SMG. (transhumanist.ru)
  • 5. Interferon-α2b gene-modified human bone marrow mesenchymal stem cells inhibit hepatocellular carcinoma by reducing the Notch1 levels. (nih.gov)
  • DNA damage exists in mouse inner medullas in vivo under the normal condition of high NaCl and urea. (nih.gov)
  • We found that RNA-Seq identified more differentially expressed genes and correlated with RT-qPCR quantification better than previously published microarray data. (molvis.org)
  • Moreover, the damage is less if the cells are not proliferating rapidly (Zhang, et al, 2002) and if the changes in NaCl and urea concentration are slow (Cai, et al, 2002, 2004), as occurs in the renal inner medulla in vivo. (nih.gov)
  • Recent findings are that high NaCl causes DNA damage and impairs DNA repair, not only in cell culture, but also in vivo (Dmitrieva, et al, 2003). (nih.gov)