Ischemia reperfusion injuryOxidative StressCardiacMyocardial InfarctionInhibitionIschaemiaInflammationMiceVitro and in vivoNormothermicRatsInfarct sizeMyocardiumCardiomyocytesFree radicalsIschemic injuryTransplantationOccursHypoxiaEndothelial cellsNecrosisInflammatoryNitricMimicsPathwaysDonorAbstractTissueBiochemicalModelDysfunctionClinicalLiver graftTherapeuticAlleviatePreservationAssessmentDamageCardioprotectiveMethodsEpithelialFerroptosisFlapsMechanismsFunctionalProtects
Ischemia reperfusion injury20
- Renal ischemia-reperfusion injury (IRI) is a major cause of acute kidney injury (AKI). (mdpi.com)
- In vivo quantification of VCAM-1 expression in renal ischemia reperfusion injury using non-invasive magnetic resonance molecular imaging. (ox.ac.uk)
- RATIONALE AND OBJECTIVE: Vascular cell adhesion molecule-1 (VCAM-1) is upregulated in ischemia reperfusion injury (IRI), persisting after restoration of blood flow. (ox.ac.uk)
- Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart. (ox.ac.uk)
- ischemia/reperfusion injury (I/R injury). (nih.gov)
- Renal ischemia-reperfusion injury (IRI) is considered as a major cause of acute kidney injury. (frontiersin.org)
- Renal ischemia-reperfusion injury (IRI) is a common pathophysiological phenomenon in clinical settings. (frontiersin.org)
- The purpose of this study was to examine our hypotheses that BoTA provide protection of musculocutaneous flap from ischemia-reperfusion injury. (hindawi.com)
- Ischemia-reperfusion injury occurs when circulation is abruptly restored following prolonged ischemia and it is well-known that high levels of calcium and tissue neutrophil accumulation cause cellular damage and produce reactive oxygen species (ROS) during reperfusion and trigger IR injury [ 1 ]. (hindawi.com)
- Recent studies have shown that ferroptosis is closely related to the pathophysiological processes of many diseases, such as tumors, nervous system diseases, ischemia-reperfusion injury, kidney injury, and blood diseases. (nature.com)
- The authors found that liver grafts with absent carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) exhibited increased ischemia-reperfusion injury inflammation and decreased function in wild-type recipients. (jci.org)
- The preclinical work focuses on animal models of myocardial ischemia-reperfusion injury, which is an experimental model for STEMI, as well as ex-vivo models of myocardial ischemia. (gu.se)
- The volatile anesthetic desflurane (DES) effectively reduces cardiac infarct size following experimental ischemia/reperfusion injury in the mouse heart. (uni-wuerzburg.de)
- Aromatase metabolizes testosterone to 17b- estradiol (E2) and thereby significantly contributes The volatile anesthetic desflurane (DES) effectively reduces cardiac infarct size following experimental ischemia/reperfusion injury in the mouse heart. (uni-wuerzburg.de)
- It has previously been demonstrated that phosphatidylinositol‑3‑kinase (PI3K)/Akt and cleaved caspase‑3 serve critical roles in the apoptosis of cardiac myocytes following ischemia/reperfusion injury. (spandidos-publications.com)
- Moens AL, Claeys MJ, Timmermans JP and Vrints CJ: Myocardial ischemia/reperfusion-injury, a clinical view on a complex patho-physiological process. (spandidos-publications.com)
- Scarabelli TM, Stephanou A, Pasini E, et al: Different signaling pathways induce apoptosis in endothelial cells and cardiac myocytes during ischemia/reperfusion injury. (spandidos-publications.com)
- These cells are particularly vulnerable to ischemia-reperfusion injury and damage from antidonor antibodies. (tts.org)
- Cardiac ischemia-reperfusion injury (IRI) occurs intra-operatively during heart transplantation (HTx), underpinning graft survival. (uwo.ca)
- Cardiac ischemia-reperfusion injury (IRI) occurs when transplanting a heart from donor to recipient. (uwo.ca)
Oxidative Stress4
- However, harmful stimuli (such as ischemia-reperfusion, oxidative stress, and toxic chemicals) can change the direction and efficiency of intercellular mitochondrial transfer. (frontiersin.org)
- Livers treated with NMP combined with BMMSCs showed better liver function, relieved histopathological damage, reduced oxidative stress injury and ferroptosis, and the mechanism of reduction was associated with downregulation of intracellular reactive oxygen species (ROS) and free Fe²⁺ levels. (medscimonit.com)
- We propose that EUK-134 reduces renal I/R injury not only via reduction of oxidative stress, but also by reducing nitrosative stress caused by renal I/R. (scienceopen.com)
- One of the primary causes of ARF is ischemia/reperfusion (I/R). Inflammatory process and oxidative stress are thought to be the major mechanisms causing I/R. MK-886 is a potent inhibitor of leukotrienes biosynthesis which may have anti-inflammatory and antioxidant effects through inhibition of polymorphonuclear leukocytes (PMNs) infiltration into renal tissues. (biomedcentral.com)
Cardiac11
- Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. (ox.ac.uk)
- Epigallocatechin‑3‑gallate (EGCG), the predominant catechin component of green tea, has been reported to have potential cardioprotective effects in primary cultures of cardiac myocytes exposed to I/R injury, mediated through inhibition of signal transducers and activators of transcription‑1 activity. (spandidos-publications.com)
- In the present study, the protective effects of EGCG with zinc were assessed on cultures of rat cardiac myoblasts exposed to hypoxia/reoxygenation (H/R) injury. (spandidos-publications.com)
- Since EGCG + Zn2+ may, at least in part, protect cardiac myocytes against H/R‑induced apoptotic cell death, the PI3K/Akt pathway of EGCG may be enhanced by its interactions with zinc during H/R injury. (spandidos-publications.com)
- Stephanou A, Brar B, Liao Z, et al: Distinct initiator caspases are required for the induction of apoptosis in cardiac myocytes during ischaemia versus reperfusion injury. (spandidos-publications.com)
- Townsend PA, Scarabelli TM, Pasini E, et al: Epigallocatechin-3-gallate inhibits STAT-1 activation and protects cardiac myocytes from ischemia/reperfusion-induced apoptosis. (spandidos-publications.com)
- This is known as the "reperfusion" phase in cardiac IRI. (uwo.ca)
- Short-term cardiac stress, induced by ischemia-reperfusion (I/R) injury resulted in impaired left ventricular (LV) recovery and increased infarct size in heterozygous Hmox1-deficient (Hmox1 +/− ) mice [ 55 ]. (springer.com)
- Cardiac function in vivo was assessed by echocardiography . (bvsalud.org)
- When cardiac MRI came around, around the year 2000, suddenly there was a noninvasive imaging method where we could investigate patients in vivo," said Kumar. (medscape.com)
- If treated with cardiopulmonary resuscitation, however, cardiac arrest is survivable, but survivors often show evidence of injury in selectively vulnerable regions of the brain. (cdc.gov)
Myocardial Infarction2
- Cannon RO III: Mechanisms, management and future directions for reperfusion injury after acute myocardial infarction. (spandidos-publications.com)
- The Canadian Cardiovascular Society (CCS) has developed a four-stage classification of acute atherothrombotic myocardial infarction (MI) based on the severity of the injury to the myocardium. (medscape.com)
Inhibition2
- This inhibition is abolished following reperfusion injury or inhibition of NO synthesis. (eurekamag.com)
- APC reduces the pulmonary endothelial cell injury and hypotension in rats administered endotoxin (ET) by inhibiting TNF-α production through inhibition of its transcription. (eurekaselect.com)
Ischaemia5
- In group 2 the response to acetylcholine was significantly (p lt 0.05) attenuated in left circumflex coronary arteries exposed to ischaemia and reperfusion compared with left anterior descending control rings from the same heart. (eurekamag.com)
- Proximal left circumflex vessels exposed to ischaemia and reperfusion showed enhanced sensitivity to the relaxant effects of SIN-1 compared to control proximal vessels obtained from the same hearts. (eurekamag.com)
- Conclusions: Endothelium dependent vasodilatation is attenuated by ischaemia and reperfusion in both proximal and distal coronary arteries of the size studied. (eurekamag.com)
- Tao Q, Tianyu W, Jiangqiao Z, Zhongbao C, Xiaoxiong M, Long Z, Jilin Z. Tripartite Motif 8 Deficiency Relieves Hepatic Ischaemia/reperfusion Injury via TAK1-dependent Signalling Pathways. (ijbs.com)
- However, the function of Trim8 in hepatic ischaemia/reperfusion (I/R) injury remains largely unknown. (ijbs.com)
Inflammation1
- Trim8 knockout relieved hepatocyte injury triggered by I/R. Silencing of Trim8 expression alleviated hepatic inflammation responses and inhibited apoptosis in vitro and in vivo . (ijbs.com)
Mice7
- METHODOLOGY AND FINDINGS: Mice subject to unilateral, transient (30 minutes) renal ischemia and subsequent reperfusion received intravenous VCAM-MPIO (4.5 mg iron/kg body weight). (ox.ac.uk)
- Cordycepin has been shown to have anti-inflammatory qualities, as well as the ability to defend against injury from cerebral ischemia in mice. (wikipedia.org)
- The results indicated that Trim8 was significantly upregulated in liver of mice subjected to hepatic I/R injury. (ijbs.com)
- In vivo, C57/BL6 mice received heterotopic HTx with/without DLC1β-OE plasmid tail-vein injections. (uwo.ca)
- A total of 24 Adult males of Swiss albino mice were randomized to four groups: I/R group (n = 6), mice underwent 30 minute bilateral renal ischemia and 48 hr reperfusion. (biomedcentral.com)
- After the end of reperfusion phase mice were sacrificed, blood samples were collected directly from the heart for determination of serum TNF-a, IL-6, urea and Creatinine. (biomedcentral.com)
- Isolated ex vivo mice hearts were perfused in a constant pressure Langendorff mode, and exposed to 30min of global ischemia (GI) and 60min of reperfusion. (uib.no)
Vitro and in vivo1
- In vitro and in vivo animal studies also demonstrate that rosuvastatin exerts vasculoprotective effects independent of its lipid-lowering properties. (pediatriconcall.com)
Normothermic4
- Quantification of cell density indicated no significant edema in the myometrium or in the endometrium of uteri exposed to 4 hours cold ischemia and then a normothermic ex vivo reperfusion for 48 hours. (nih.gov)
- To mitigate obligate reperfusion injury in VCA, we evaluate the efficacy of a novel normothermic hyperbaric oxygen warm ex-vivo perfusion strategy using hyperoxygenated University of Wisconsin (UW) solution in a porcine VCA model. (sages.org)
- Hyperbaric normothermic perfusion dramatically extends the viability of composite tissues ex-vivo. (sages.org)
- This new technique, ex vivo normothermic machine perfusion (NMP) could increase the number of viable organs available for transplantation. (tts.org)
Rats6
- In the present work, a hypoxia/reoxygenation (H/R) model in NRK-52E cells and ischemia-reperfusion model in rats were used. (mdpi.com)
- The results showed that the TFs significantly attenuated cell injury and markedly decreased serum creatinine (Cr) and blood urea nitrogen (BUN) levels in rats. (mdpi.com)
- Furthermore, APC reduces the ischemia / reperfusion-induced renal injury and the stress-induced gastric mucosal injury in rats. (eurekaselect.com)
- Furthermore, APC reduced the spinal cord injury induced by compression-trauma or ischemia / reperfusion by inhibiting TNF-α production in rats, suggesting that APC may be a potential therapeutic agent for spinal cord injury in which only limited therapeutic measures are currently available. (eurekaselect.com)
- Rats, subjected to bilateral renal ischemia (45 min) followed by reperfusion (6 h), were administered EUK-134 (0.3 and 3 mg/kg, i.v.) prior to and during reperfusion, after which biochemical and histological indicators of renal dysfunction and injury were measured. (scienceopen.com)
- However, EUK-134 also reduced nitrosative stress caused by I/R in vivo (reduction of iNOS expression and nitrotyrosine formation), which was reflected by a significant reduction in serum NO levels in rats subjected to renal I/R. Specifically, serum NO levels were reduced from 57 ± 12 (n = 12, I/R only) to 23 ± 3 m M (n = 12, I/R +3 mg/kg EUK-134). (scienceopen.com)
Infarct size1
- Anastrozole blocked DES induced preconditioning and increased infarct size compared to DES alone (37.94615.5% vs. 17.163.62%) without affecting area at risk and systemic hemodynamic parameters following ischemia/reperfusion. (uni-wuerzburg.de)
Myocardium1
- This study explores if long-term feeding of an obesogenic high fat diet renders the myocardium less susceptible to ischemic-reperfusion induced injury via Epac-dependent signaling. (uib.no)
Cardiomyocytes3
- Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
- Cardiomyocytes were isolated and subjected to 6 h hypoxia followed by 18 h reperfusion. (biomedcentral.com)
- In vitro, HL-1 cells (mouse cardiomyocytes) received chamber hypoxia-oxygenation reperfusion (H/R) for 24H4R with/without DLC1β plasmid, before collection for protein/mRNA analyses. (uwo.ca)
Free radicals3
- The microvascular and parenchymal organ damage induced upon ischemia tissue reperfusion is mainly attributed to the reactive oxygen-free radicals, and it has been demonstrated in many organs. (frontiersin.org)
- The production of antioxidant enzymes that scavenge free radicals in ischemic tissue is then impaired, thereby exacerbating the damage caused by these free radicals in the post ischemic reperfusion tissue. (frontiersin.org)
- Activation of Kupffer cells and neutrophils leads to release of inflammatory cytokines and free radicals, which further aggravate liver injury [ 4 , 5 ]. (ijbs.com)
Ischemic injury3
- Histological evaluation revealed extensive diffuse evidence of necrosis in all controls (at 3 hours, cold static preservation) but flaps placed on hyperbaric ex-vivo perfusion support showed decreased histologic evidence of ischemic injury or necrosis ranging from rare to moderate. (sages.org)
- While extending criteria for liver organs provides a needed resource, tissue damage from prolonged ischemic injury can result in early allograft dysfunction and consequent rejection. (jci.org)
- Scavenging peroxynitrite using seleno-organic compounds like ebselen provides renoprotection against ischemic injury. (scienceopen.com)
Transplantation7
- We, therefore, aimed to develop an ex vivo sheep uterus reperfusion platform that mimics the reperfusion situation so that initial assessments and comparisons can be performed without the need for costly and labor-intensive in vivo transplantation experiments. (nih.gov)
- The experimental setup is a platform that can be used to conduct further studies on uterine ischemia- and reperfusion injury that may lead to improved human uterus transplantation protocols. (nih.gov)
- The injury an organ sustains during recovery, preservation, and transplantation occurs primarily as a result of ischemia and hypothermia. (medscape.com)
- used a mouse transplantation model with prolonged ex vivo cold storage to explore liver graft protection. (jci.org)
- Ischaemic reperfusion (I/R) occurs during surgical procedures, including hepatic resection and liver transplantation (LT) [ 1 ]. (ijbs.com)
- The conducted study focuses on how we can reduce injury to the heart during heart transplantation. (uwo.ca)
- By analyzing these questions, this research might be useful in humans to potentially reduce the rate of injury during heart transplantation. (uwo.ca)
Occurs1
- Necrotizing enterocolitis (NEC), which typically occurs in the second to third week of life in premature, formula-fed infants, is characterized by variable damage to the intestinal tract, ranging from mucosal injury to full-thickness necrosis and perforation (see the image below). (medscape.com)
Hypoxia2
- H9c2 cells were subjected to 3‑h hypoxia, followed by 1‑h reperfusion. (spandidos-publications.com)
- Previous studies of global brain hypoxia ischemia have primarily focused on injury to the cerebral cortex and to the hippocampus. (cdc.gov)
Endothelial cells1
- A key approach is the application of confocal intravital microscopy that enables rigorous and direct means of investigating the interactions of leukocytes with different components of microvessel walls (endothelial cells, pericytes and the vascular basement membrane) in real-time in vivo. (qmul.ac.uk)
Necrosis2
- MI with significant cardiomyocyte necrosis but without microvascular injury. (medscape.com)
- Cardiomyocyte and microvascular necrosis leading to reperfusion hemorrhage. (medscape.com)
Inflammatory1
- These observations strongly suggest that APC might be involved not only in regulation of the coagulation system, but in regulation of inflammatory responses by preventing endothelial cell injury. (eurekaselect.com)
Nitric4
- The Janus face of nitric oxide (NO) has prompted a debate as to whether NO plays a deleterious or protective role in tissue injury. (researchgate.net)
- The drug also modulates nitric oxide synthase (NOS) expression and reduces ischemic-reperfusion injuries in rat hearts (PMID: 15914111). (pediatriconcall.com)
- This overview provides information on the pathophysiology of the inducible nitric oxide synthase/nitric oxide (iNOS/NO) system in the injury to cultured renal tubular epithelia, freshly isolated proximal tubules, and the whole organ after hypoxic or ischemic insult. (scienceopen.com)
- In conclusion, the intricate relations between endothelial and epithelial cells, based in part on the relations between endothelial and inducible nitric oxide synthases, are perturbed in renal ischemia primarily as a result of endothelial dysfunction precipitating epithelial injury. (scienceopen.com)
Mimics1
- Therefore, our aim was to develop a medium throughput comorbidity cell-based test system of myocardial I/R injury, hypercholesterolemia and hyperglycemia that mimics comorbidity conditions. (nih.gov)
Pathways1
- In conclusion, our study demonstrates that Trim8 deficiency plays a protective role in hepatic I/R injury by inhibiting the activation of TAK1-dependent signalling pathways. (ijbs.com)
Donor1
- BMMSCs showed significant protective effects on the ultrastructure of DCD donor livers and ROS-induced injury to IAR-20 cells under electron microscopy. (medscimonit.com)
Abstract1
- Abstract Compound Danshen Dripping Pills (CDDPs) have been used in clinical treatment to protect the heart from ischemia / reperfusion (IR) injury for many years. (bvsalud.org)
Tissue1
- Neutrophils are the first immune cells that infiltrate the damaged tissue as early as 24 h after injury. (springer.com)
Biochemical2
- Histological and biochemical analysis of the perfusate was conducted to assess reperfusion injury. (nih.gov)
- In addition to these chemical and biochemical properties, NO can modulate cellular and physiological processes to limit oxidative injury, limiting processes such as leukocyte adhesion. (researchgate.net)
Model3
- We developed a novel ex vivo sheep uterus model for prolonged perfusion. (nih.gov)
- This model proved to be able to distinguish reperfusion injury-related differences associated to organ preservation. (nih.gov)
- In this study, we found that local BoTA preconditioning yielded significant protection against IR injury in a rat musculocutaneous flap model. (hindawi.com)
Dysfunction4
- Hypercholesterolemia causes endothelial and myocardial dysfunction, as well as aggravates ischemia/reperfusion (I/R)-induced myocardial injury. (nih.gov)
- Here we investigate the effect of EUK-134, a synthetic superoxide dismutase and catalase mimetic, (i) on renal dysfunction and injury caused by I/R in vivo and (ii) on proximal tubular cell (PTC) injury and death caused by oxidative and nitrosative stress. (scienceopen.com)
- EUK-134 produced a significant reduction in renal dysfunction and injury caused by I/R. Specifically, serum creatinine levels, an indicator of renal dysfunction, were reduced from 227 ± 11 (n = 12, I/R only) to 146 ± 9 µ M (n = 12, I/R +3 mg/kg EUK-134). (scienceopen.com)
- These sequelae of renal ischemia are a result of endothelial dysfunction, which is most probably responsible for the 'no-reflow' phenomenon and further aggravation of tubular ischemia during the early reperfusion period. (scienceopen.com)
Clinical3
- EGCG and zinc may therefore represent effective agents for use in the prevention of I/R injury in clinical practice. (spandidos-publications.com)
- I/R is a common pathological disorder leading to acute liver injury, a potentially life-threatening clinical condition with high morbidity, mortality, and prolonged patient hospitalization. (ijbs.com)
- Acute kidney injury (AKI) is a common clinical syndrome characterized by rapid deterioration of renal function. (biomedcentral.com)
Liver graft1
Therapeutic1
- A therapeutic drug that targets ischemia reperfusion (I/R) injury is needed and has yet to be developed. (biomedcentral.com)
Alleviate3
- In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)
- Many studies have tried to discover pharmacologic or surgical interventions that may alleviate IR flap injuries [ 2 - 13 ]. (hindawi.com)
- The toxin appears to not only have a positive effect on muscle circulation and vessels [ 20 - 23 ] but also apparently have the ability to alleviate IR injury in muscle flaps [ 6 , 24 ]. (hindawi.com)
Assessment1
- We hypothesized that microparticles of iron oxide targeting VCAM-1 (VCAM-MPIO) would depict "ischemic memory" and enable in vivo assessment of VCAM-1 expression. (ox.ac.uk)
Damage2
- However, a much faster and severe reperfusion damage of all uterine layers were evident during the reperfusion experiment following 48 hours of cold ischemia. (nih.gov)
- However, if we can understand how this damage happens, we may be able to develop strategies to avoid this injury in future. (uwo.ca)
Cardioprotective1
- Endogenous cardioprotective mechanisms against I/R are impaired in hyperlipidemic and hyperglycemic in vivo animal models. (nih.gov)
Methods1
- Methods: In vitro reactivity of preconstricted coronary arterial rings was studied in control dogs (group 1), and dogs in which the left circumflex coronary artery was subjected in vivo to four acute occlusions of 5 min duration, with three intervening reperfusion periods of 5 min and a final reperfusion period of 60 min (group 2). (eurekamag.com)
Epithelial1
- Only the outer serosa layer and the inner columnar luminal epithelial cells were affected by the reperfusion. (nih.gov)
Ferroptosis1
- Ferroptosis plays an important regulatory role in the occurrence and development of many diseases, such as tumors, neurological diseases, acute kidney injury, ischemia/reperfusion, etc. (nature.com)
Flaps1
- While studies using various materials to overcome ischemia-reperfusion (IR) injury are becoming increasingly common, studies on the effects of botulinum toxin A (BoTA) on IR injury in musculocutaneous flaps are still limited. (hindawi.com)
Mechanisms1
- The attenuation of metal/peroxide oxidative chemistry, as well as lipid peroxidation, appears to be the major chemical mechanisms by which NO may limit oxidative injury to mammalian cells. (researchgate.net)
Functional1
- In contrast, functional consequences of injury to the thalamus are poorly understood but may be crucially important. (cdc.gov)
Protects1
- To the best of our knowledge, the current study was the first to demonstrate that EGCG + Zn2+ protects H9c2 cells against H/R injury through activation of the PI3K/Akt pathway, as determined by western blotting. (spandidos-publications.com)