Chem-seq can be carried out either In vitro or In vivo, although the results from each have proven to be highly similar. (wikipedia.org)
Juice of eclipta prostrata inhibits cell migration in vitro and exhibits anti-angiogenic activity in vivo. (cri.or.th)
TRAIL induces apoptosis in a variety of cell lines in vitro and in vivo . (biomedcentral.com)
TXA improves IMQ-induced psoriasis-like inflammation in vivo and psoriatic keratinocytes in vitro. (bvsalud.org)
Intestinal organoids are three-dimensional (3D) in vitro tissue cultures that model the in vivo intestine. (stemcell.com)
PRP was tested in vivo , after bovine intrauterine administration, and in vitro on endometrial cells. (biomedcentral.com)
For this, the in vitro activity of four non-dihydropyridine agents (amrinone, fendiline, mibefradil, and lidoflazine) was tested against different Leishmania species and their cytotoxicity to mammalian cells was evaluated. (hindawi.com)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
In this study, we tried to examine the anti-metastasis and apoptosis/autophagy actions of Coenzyme Q0 (CoQ0, 2,3-dimethoxy-5-methyl-1,4-benzoquinone), a derivative of Antrodia camphorata in HNCC TWIST1 overexpressing (FaDu-TWIST1) cells as well as in vivo tumor xenograft mice model. (bvsalud.org)
BN80927 shows high efficiency in vivo in tumor xenograft studies using human androgen-independent prostate tumors PC3 and DU145. (aacrjournals.org)
Apoptosis3
Experimental approaches include delivery of oncolytic viruses, immunomodulatory molecules, p53 and p21, enzymes that metabolize prodrugs and agents that can induce apoptosis [ 3 ]. (biomedcentral.com)
One agent that has received considerable attention as a novel apoptosis-inducing agent is tumor necrosis factor-related apoptosis inducing ligand (TRAIL/Apo2L). (biomedcentral.com)
Many chemotherapeutic agents are designed to inhibit growth or induce apoptosis in malignant cells by inducing DNA damage. (precisionformedicine.com)
Antiviral1
LJ001 did not mediate any apparent damage on biogenic cellular membranes, likely due to multiple endogenous cytoprotection mechanisms against phospholipid hydroperoxides.Based on our understanding of LJ001's mechanism of action, we designed a new class of membrane-intercalating photosensitizers to overcome LJ001's limitations for use as an in vivo antiviral agent. (utmb.edu)
Therapeutic agents3
The Children's Oncology Group (COG) in North America, Ireland, New Zealand, and Australia and other cooperative groups have viewed relapse as an opportunity to test novel therapeutic agents efficiently in smaller cohorts of patients via early-phase clinical trials. (haematologica.org)
The control of gene expression by therapeutic agents has great potential for the field of neurological diseases. (scientificarchives.com)
A wide variety of nano-sized drug carriers has found niche in the field, owing to their unique structures which give rise to new generations of therapeutic agents and medical devices [ 1 ]. (biomedcentral.com)
Molecules1
In vivo Chem-seq During In vivo Chem-seq, cultured cells in medium are treated simultaneously with either a biotinylated version of the small molecule under study or DMSO (as a control) and 1% formaldehyde for the crosslinking of DNA, proteins and small molecules. (wikipedia.org)
Antimicrobial Agents1
Fungal biofilm-associated infections are frequently refractory to conventional therapy because of resistance to antimicrobial agents. (cdc.gov)
Chemotherapeutic agent2
Doxorubicin (Dox), a non-selective class I anthracycline antibiotic, is a potent chemotherapeutic agent which is used for the treatment of numerous cancers [ 1 ]. (oncotarget.com)
Doxorubicin, which has been the most frequently used chemotherapeutic agent in the treatment of soft tissue sarcomas, demonstrates response rates of 20-30% in disseminated disease ( 9 , 10 ). (spandidos-publications.com)
Assay2
Preclinical data reported here show that BN80927 retains Topo I poisoning activity in cell-free assay (DNA relaxation) as well as in living cells, in which in vivo complexes of topoisomerase experiments and quantification of DNA-protein-complexes stabilization, have confirmed the higher potency of BN80927 as compared with the Topo I inhibitor SN38. (aacrjournals.org)
To monitor the extent of DNA damage and ultimately the efficacy of topotecan or similar agents, ApoCell was developed a biomarker assay for the detection of the expression levels of γH2AX. (precisionformedicine.com)
Potent3
In cell growth assays, BN80927 is a very potent antiproliferative agent as shown by IC 50 values consistently lower than those of SN38 in tumor cell lines as well as in their related drug-resistant lines. (aacrjournals.org)
GML is a potent antimicrobial agent that targets a range of bacteria, fungi, and enveloped viruses but select findings suggest that GML also has immunomodulatory functions. (nature.com)
19 published his findings on cortisone causing dramatic improvements in patients with rheumatoid arthritis, corticosteroids have become established as the most potent anti-inflammatory agents in the pharmacotherapy of various chronic inflammatory diseases, including asthma 20 . (ersjournals.com)
Studies5
Techasakul, S. Synthesis, Cytotoxicity Evaluation, and Molecular Modeling Studies of 2,N10-Substituted Acridones as DNA-Intercalating Agents. (cri.or.th)
For in vivo studies, PC3 prostate carcinoma cells were grown subcutaneously in athymic nude mice and tumor growth was measured following treatment with doxorubicin and/or Apo2L/TRAIL. (biomedcentral.com)
As the technology has matured, intestinal organoids have found application in a wide variety of research fields, including upstream drug discovery and patient-specific drug screening, cancer and immunology studies, and the pathogenesis of infectious agents. (stemcell.com)
Studies involving mechanism of emodin, an herbal extract, has revealed its potential as anti-inflammatory, antioxidant and anti-cancer agent. (longdom.org)
These data demonstrated that non-dihydropyridine-CCBs present antiprotozoal activity and could be useful candidates for future in vivo efficacy studies against Leishmaniasis and Chagas' disease. (hindawi.com)
Prostate1
Combination of doxorubicin and Apo2L/TRAIL is more effective in growth inhibition of PC3 xenografts in vivo than either agent alone and could present a novel treatment strategy against hormone-refractory prostate cancer. (biomedcentral.com)
Efficacy1
The in vivo oral efficacy of amlodipine and lacidipine has been shown in the treatment of Leishmania (L.) donovani infected mice [ 5 ]. (hindawi.com)
Successfully1
Powder x-ray diffraction (XRD), Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA) confirmed the drug anions were successfully intercalated in the interlayer space of LZH. (biomedcentral.com)
Antifungal4
The effectivity of Indonesian propolis from Tetragonula biroi bee as an antifungal agent for Candida sp. (ui.ac.id)
Biofilm-associated Candida show uniform resistance to a wide spectrum of the currently available conventional antifungal agents, which implies that antimicrobial drugs that specifically target biofilm-associated infections are needed. (cdc.gov)
The novel classes of antifungal agents, the lipid formulation of amphotericins, and the echinocandins have demonstrated unique antifungal activity against the resistant Candida biofilms, providing a breakthrough in the treatment of life-threatening invasive systemic mycoses. (cdc.gov)
Although C. albicans is the predominant etiologic agent of candidiasis, other Candida species that tend to be less susceptible to the commonly used antifungal drugs such as C. krusei , C. glabrata , C. lusitaniae , and the newest Candida species, C. dubliniensis, have emerged as substantial opportunistic pathogens ( 10 ) . (cdc.gov)
Mice1
Methods: A contrast free angiography technique is validated in silico through the use of computer-generated images and in vivo through microcomputed tomography (μCT) of live mice conducted using a laboratory-based x-ray source. (excillum.com)
Cells6
Chem-seq was tested on three classes of drugs using MM1.S multiple myeloma cells to: 1) Investigate the genome-wide binding of the bromodomain inhibitor JQ1 to the BET bromodomain family members BRD2, BRD3 and BRD4 2) Map the genomic binding sites of AT7519, an inhibitor of the cyclin dependent kinase CDK9, and 3) Study how the DNA intercalating agent psoralen interacts with genomic DNA in vivo. (wikipedia.org)
When dsRNA is administered in vivo, recipient cells sense it as an invading virus. (bioprocessintl.com)
Extracorporeal photochemotherapy involves the ex vivo exposure of leukapheresed peripheral blood mononuclear cells to UVA in the presence of 8-MOP (DNA-intercalating agent) and subsequent reinfusion of the treated cells. (medscape.com)
After optimizing scan settings between the two sites, NCI-H460 cells were treated with gemcitabine, another DNA damaging agent. (precisionformedicine.com)
Our data reveal that the widely used anti-microbial agent GML also alters the lipid dynamics of human T cells, leading to their defective signaling and function. (nature.com)
18 At the base of the intestinal crypt, the ISCs are found intercalated with Paneth cells, 17 credited with much of the signaling required to maintain the stem cell niche. (stemcell.com)
Molecular2
Consequently, plenty of strategies were developed to increase the therapeutic duration of BF-30 in vivo including enlarging the molecular size via PEGylation to prevent from the rapid renal filtration and enzymatic degradation [ 6 ]. (jcancer.org)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)
Cellular1
Paralleling the emergence of targeted therapeutics in cancer treatment, a variety of experimental approaches has led to the conclusion that tumors bearing some specific genetic alterations may lead them to be uniquely sensitive to agents deregulating their cellular metabolism. (aacrjournals.org)
Chemotherapy1
1-4 These survival gains have been attributed largely to advances in biological and genetic characterization of heterogeneous pediatric AML subtypes via next-generation sequencing with clinical outcome correlation, and to enhanced supportive care measures focused on reducing toxicities from intensive multi-agent chemotherapy regimens required for cure. (haematologica.org)
Activity1
This agent acts by inhibiting inflammatory reactions and pain via decreasing the activity of cyclooxygenase, which results in a decrease of prostaglandin synthesis. (medscape.com)
Tissue1
Few agents such as doxorubicin, ifosfamide and dacarbazine have proven to be effective in the therapy of soft tissue sarcomas ( 2 ). (spandidos-publications.com)
Study4
In vivo imaging of the pulmonary vasculature in small animals is difficult yet highly desirable in order to allow study of the effects of a host of dynamic biological processes such as hypoxic pulmonary vasoconstriction. (excillum.com)
Rana, R., Minhas, L.A. & Mubarik, A. Histological study of human sublingual gland with special emphasis on intercalated and striated ducts. (nature.com)
This study lays the foundations for the potential treatment of endometritis with PRP in vivo . (biomedcentral.com)
The present study aims to evaluate the in vivo anti. (researchgate.net)
Resistance1
Cross-resistance to intercalating agents in an epipodophyllotoxin-resistant Chinese hamster ovary cell line: evidence for a common intracellular target. (mcmaster.ca)
Enzymes1
Plasmids and enzymes can be sources of contaminants and adventitious agents. (bioprocessintl.com)
Detection2
The intercalating fluorescent dye PicoGreen (Thermo Fisher Scientific) was added to the sample in advance to increase sensitivity of DNA detection ( 2 ). (bioprocessintl.com)
In vivo treatment with PRP increased the detection of PR. (biomedcentral.com)
Class1
The major pharmacologic action of agents in this class is noncompetitive inhibition of the enzyme carbonic anhydrase. (medscape.com)
Treatment1
Cross-calibration of the LSC instruments at both test sites was completed to determine equivalent scan settings, and then the effects of treatment with a DNA damaging agent on a cell line was compared at both locations, as seen in Figure 2. (precisionformedicine.com)
Development1
Design and development of nanocarriers packaged with gene-lowering agents represents a significant advance towards non-invasive nose-to-brain delivery of gene therapy for HD and other hereditary brain disorders. (scientificarchives.com)
Shown1
In vivo delivery of ASO or RNAi has been shown to be effective in animal models of Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), spinocerebellar ataxia (SCA), and HD [1-2]. (scientificarchives.com)
Host1
CFX anions were slowly released over 80 hours in phosphate-buffered saline (PBS) solution due to strong interactions that occurred between the intercalated anions and the host lattices. (biomedcentral.com)
Viruses1
Besides maintaining human health, different sources reported that riboflavin can inhibit or inactivate the growth of different pathogens including bacteria, viruses, fungi and parasites, highlighting the possible role of riboflavin as an antimicrobial agent. (bioseek.eu)
Cell2
Topotecan, an inhibitor of DNA isomerases, intercalates between DNA bases and causes double strand breaks (DSBs), and the absence of DNA repair leads to apoptotic cell death. (precisionformedicine.com)
These organoids include a polarized epithelial layer that surrounds a functional lumen and contains cell types of the intestinal epithelium present in proportions and relative spatial arrangement that mimic what is observed in vivo. (stemcell.com)
Values2
1998. Documentation of the threshold limit values for chemical substances and physical agents. (cdc.gov)
2000. Threshold limit values for chemical substances and physical agents and biological exposure indices. (cdc.gov)