Vitro tumor cells cultured. Medical search. Frequent questions

Macrophages are emerging as major cellular factors in the tumor ecosystem that can influence the stem phenotype and cancer progression. (nature.com)
It is within the cell-autonomous context that ST6GAL1 involvement in cancer progression has been interpreted. (nature.com)
Cancer cell-extrinsic mechanisms, which are poorly understood, are also believed to contribute to disease progression and the heterogeneous genetic mutations with diverse presentations. (nature.com)
In particular, he aims to understand non-oncogene addiction mechanism that cooperates with the Ras oncogene to support tumor initiation, progression and metastasis. (cancer.gov)
This heterogeneity has been proposed to be responsible for tumour progression, metastasis and resistance to therapy. (fchampalimaud.org)
Our goal is to understand the contribution of the different tumour cell populations and genetic alterations to cancer progression and response to therapy. (fchampalimaud.org)
Importantly, cell-associated and tumor microenvironment glycosaminoglycans (GAGs)/proteoglycan (PG) content and distribution are markedly altered during tumor pathogenesis and progression. (hindawi.com)
It is now increasingly recognized that the microenvironment plays a critical role in the progression of tumors. (hindawi.com)
Cell-associated and tumor microenvironment GAG content and distribution is markedly altered during tumor pathogenesis and progression [ 11 , 12 ]. (hindawi.com)
In the local tumour microenvironment, elevated glucose levels, the presence of tumour-associated stellate cells and macrophages are hypothesised to promote the tumour progression and invasion. (lu.se)
β-catenin, when translocating to and accumulating in the nucleus, induces transcription of other oncogenes involved in tumor progression, malignancy and metastasis ( 15 ). (spandidos-publications.com)
The technical success rate, complications, local tumor progression (LTP) and overall survival (OS) were observed, and the effect of synchronous percutaneous CNB and MWA were evaluated. (bvsalud.org)

CSCs are enriched near macrophages, particularly near macrophage-containing intravasation sites called Tumor Microenvironment of Metastasis (TMEM) doorways. (nature.com)
In the metabolic symbiosis model, these cells have been proposed to overexpress MCT4 to release high amounts of lactate produced during anaerobic glycolysis into the tumor microenvironment. (hindawi.com)
This study provides fundamental insight into the functional impact of CDK4 inhibition on cells in the tumor microenvironment, which is of high importance and interest to the field. (elifesciences.org)
CAT-179 was resistant to TGFβ-mediated immunosuppression in vitro , indicating that the cells will be protected from TGFβ-mediated immune suppression in the tumor microenvironment. (pipelinereview.com)
The consecutive stages of cancer growth and dissemination are obligatorily perpetrated through specific interactions of the tumor cells with their microenvironment. (hindawi.com)
The consecutive steps of tumor growth, local invasion, intravasation, extravasation, and invasion of anatomically distant sites are obligatorily perpetrated through specific interactions of the tumor cells with their microenvironment. (hindawi.com)
In this work, bacterial cellulose (BC)/gelatin hydrogels were successfully synthesized and were investigated as scaffolds for cancer cells in vitro culture to simulate tumor microenvironment. (edu.au)
The present study investigated the influence by the microenvironment on pancreatic cancer cell invasion in vitro. (lu.se)

Cancer stem cells (CSCs) play an important role during metastasis, but the dynamic behavior and induction mechanisms of CSCs are not well understood. (nature.com)
TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
We found that SOX9 is upregulated in SWCNT-exposed cells, which is consistent with their abilities to induce tumor formation and metastasis in vivo. (cdc.gov)
Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)
Bone metastasis occurs through a complex cascade of events that ultimately results in circulating tumor cells (CTCs) from the blood extravasating and invading the bone marrow - these cells within the bone marrow are referred to as disseminated tumor cells (DTCs) [ 4 ]. (oncotarget.com)
Lymph node metastasis is responsible for the high morbidity of head and neck squamous cell carcinoma (HNSCC). (spandidos-publications.com)
In this study, we found a negative correlation of IRS-1 expression with tumor metastasis both in human tissue samples and in cell lines. (spandidos-publications.com)
Furthermore studies in tumor metastasis are quite limited and controversial. (spandidos-publications.com)
While in lung cancer, IRS-1 has been confirmed to suppress tumor metastasis ( 11 ). (spandidos-publications.com)
The epithelial-mesenchymal transition (EMT) plays important roles in tumor metastasis. (spandidos-publications.com)
Patients diagnosed with sarcomatoid clear cell renal cell carcinoma face a considerably worse prognosis due to an increased propensity for metastasis. (lu.se)

Breast cancer is one of the most common primary tumors to develop associated brain metastases ( 1 , 2 ), and while recent progress in cancer therapy has improved control of primary breast tumors, methods to effectively treat brain metastases from breast cancer remain inadequate. (jcancer.org)
In addition, metastatic sites may re-seed primary tumors in a multi-directional fashion [ 8 ]. (oncotarget.com)

TAGRISSO is indicated as adjuvant therapy after tumor resection in adult patients with non-small cell lung cancer (NSCLC) whose tumors have epidermal growth factor receptor (EGFR) exon 19 deletions or exon 21 L858R mutations, as detected by an FDA-approved test [see DOSAGE AND ADMINISTRATION ]. (rxlist.com)
On December 18, 2020, osimertinib was approved for adjuvant therapy after tumor resection in patients with non-small cell lung cancer (NSCLC) with tumors with EGFR exon 19 deletions or exon 21 L858R mutations, as detected by a U.S. Food and Drug Administration (FDA)-approved test. (ascopost.com)
Osimertinib was approved for adjuvant therapy after tumor resection in patients with NSCLC with tumors with EGFR exon 19 deletions or exon 21 L858R mutations. (ascopost.com)

CSCs have an enhanced ability to self-renew, which makes them uniquely capable of initiating and sustaining primary and metastatic tumor growth 3 . (nature.com)
SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)
Tagrisso (osimertinib) is a kinase inhibitor indicated for the treatment of patients with metastatic epidermal growth factor receptor ( EGFR ) T790M mutation-positive non-small cell lung cancer (NSCLC), as detected by an FDA-approved test, who have progressed on or after EGFR TKI therapy. (rxlist.com)
TAGRISSO is indicated for the first-line treatment of adult patients with metastatic NSCLC whose tumors have EGFR exon 19 deletions or exon 21 L858R mutations, as detected by an FDA-approved test [see DOSAGE AND ADMINISTRATION ]. (rxlist.com)
I'm a board certified neuro-oncologist who treats both primary brain tumors as well as metastatic disease to the brain and nervous system. (stanford.edu)
Specifically, these extracellular matrix (ECM) components critically modulate the tumor cell "motile phenotype" affecting their adhesive/migratory abilities which are directly correlated to the metastatic cascade [ 5 , 6 ]. (hindawi.com)

We identified kinases within the insulin signaling pathway and centrosome cycle as regulators of ependymoma cell proliferation, and their corresponding inhibitors as potential therapies. (nih.gov)
Extracellular ST6GAL1, present in cancer exosomes or the freely soluble recombinant sialyltransferase, compensates for insufficient intrinsic ST6GAL1 by boosting cancer cell proliferation and increasing invasiveness. (nature.com)
Our data indicate that extracellular ST6GAL1 from remote sources can compensate for cellular ST6GAL1-mediated aggressive tumor cell proliferation and invasive behavior and has great clinical potential for extracellular ST6GAL1 as these molecules are in the extracellular space should be easily accessible targets. (nature.com)
Our results further indicate that MCT4 promotes proliferation and survival by altered cell cycle regulation and cell death mechanisms. (hindawi.com)
For this purpose, we designed an MCT4 overexpression and knockdown/inhibition model to explore the functional consequences of MCT4 expression on cancer cell metabolism, survival, proliferation, migration, invasion potential, and angiogenesis as well as on cell cycle profiles and cell death mechanisms. (hindawi.com)
In the present study, we demonstrate that Triphala significantly inhibit the proliferation of Capan-2 and BxPC-3 human pancreatic cancer cells. (biomedcentral.com)
The cells were then treated with different concentrations of IL-17, ranging from 10 pg/mL to 100 ng/mL, and evaluated for IL-17 ability to modify tumor cell proliferation and viability through cell counting and MTT assay. (austinpublishinggroup.com)
IL-17RA mRNA expression was detected on the cell lines tested, but the incubation of IL-17 did not alter tumor cell viability or proliferation. (austinpublishinggroup.com)
The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant adhesion, proliferation, ingrowth and differentiation. (edu.au)
We demonstrate that UCN-01, at concentrations lower than those necessary to inhibit cancer cell growth, inhibit proliferation of human endothelial cells in vitro. (karger.com)
We conclude that UCN-01, at clinically relevant concentrations, exerts an anti-neovascularization effect by blocking two important steps in vessel formation: (1) the response of cancer cells to hypoxia, and (2) endothelial cell proliferation. (karger.com)
However, recently Houghton et al ( 7 ) showed increased cell proliferation after silencing of IRS1 gene expression in A549 cells suggesting tumor suppression potential. (spandidos-publications.com)
Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
Experimental in vitro and in vivo research demonstrated decreased cell proliferation in desmoid cell cultures and small tumors when COX-2 was blockaded with pharmacologic agents. (medscape.com)

Engineered cells were highly cytotoxic against multiple CD70-positive tumor cells lines derived from renal cell carcinoma (RCC) and acute myeloid leukemia (AML). (pipelinereview.com)
In addition, IL-17 enhanced the cytotoxic effects of NK cells against tumor cells [6]. (austinpublishinggroup.com)
Ongoing research investigates mechanisms invoked by cancer cells to abrogate MHC-I expression and attenuate anti-tumour CD8 + cytotoxic T cell response. (portlandpress.com)
All cases are otherwise negative for Epstein-Barr virus (EBV) (assayed using in situ hybridization for EBV-encoded ribonucleic acid [RNA] [EBER-ISH]), B-cell, T-cell, myeloid, and natural killer (NK) cell markers, as well as for cytotoxic proteins (assayed using IHC). (medscape.com)
This has led to investigations of both cytotoxic and noncytotoxic chemotherapy, but because the tumors are rarely fatal, finding a pharmacologic treatment with fewer complications is desirable, according to the authors. (medscape.com)

it claimed (through various in vivo and in vitro studies) that their compound maintained health, enhanced the immune system, and had a tonic and an anti-allergenic effect. (shamansgarden.com)
We do that by combining genetic lineage tracing, clonal analysis, imaging techniques, tumour organoid cultures and functional experiments in vivo and in vitro. (fchampalimaud.org)

Malignant cells might have an impact on the mesothelial response to applied sclerosing agents and, in consequence, on the efficacy of pleurodesis. (medscimonit.com)
Monocarboxylate transporter 4 (MCT4, SLC16A3 ) is elevated under hypoxic conditions in many malignant tumors including gliomas. (hindawi.com)
Malignant gliomas are the most common primary malignant brain tumors with an increasing incidence of up to nine per 100,000 habitants over the last years [ 1 , 2 ]. (hindawi.com)
Malignant tumors are complex tissues which arise from normal cells in a protracted multi-step process. (dkfz.de)
BTN3A, which belongs to the B7-subfamily of Ig proteins, is required for the recognition of malignant or infected cells by human g9d2 T-cells by sensing intracellular accumulation of phosphoantigens. (bmj.com)
Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)

Here, we employ high-resolution intravital microscopy using a CSC biosensor to directly observe CSCs in live mice with mammary tumors. (nature.com)
Instead of accumulating in mouse cells and halting cell division in the genetically engineered mice, the altered p53 protein performed flawlessly: it was unstable when no DNA damage was present and was stable and fully functional when needed to halt the cycle cell to repair DNA damage or to induce apoptosis. (news-medical.net)
Reduced tumor-growth in Triphala fed mice was due to increased apoptosis in the tumors cells, which was associated with increased activation of p53 and ERK. (biomedcentral.com)
Therefore, in vivo mouse models of cancer including genetically engineered mouse lines and hetero-grafted mice are indispensable tools for the exploration of the molecular and cellular fundamentals of tumor growth as well as translational applications in cancer research. (dkfz.de)
CAT-179 showed potent anti-tumor activity in vivo against a xenografted HER2+ N87 gastric cancer cell line and led to a significant survival benefit in tumor-bearing mice. (pipelinereview.com)
For bladder cancer, the tumor was reduced in IL-17 -/- mice, indicating a role of this cytokine in promoting tumor growth through IL-6/ STAT3 pathway [4]. (austinpublishinggroup.com)
Immunocompromised mice bearing human tumors and adoptively transferred with human g9d2 T cells were used to assess ICT01 anti-tumor activity in vivo. (bmj.com)
However, a body of circumstantial evi- susceptible mice by stimulating elevated levels of proin- dence links diabetes, multiple sclerosis, myocarditis, and flammatory cytokines from mast cells during the innate many other autoimmune diseases with preceding infec- immune response. (cdc.gov)
To complement the in vivo system of chick embryos, we also employ human cell cultures, chick neural crest derived in vitro crestospheres and in vivo mice models. (lu.se)

In mouse and rat xenograft tumor models, TAK-285 showed antitumor activity against cancers that expressed HER2 or EGFR. (jcancer.org)
Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)
Further, oral administration of 50 mg/kg or 100 mg/kg Triphala in PBS, 5 days/week significantly suppressed the growth of Capan-2 pancreatic tumor-xenograft. (biomedcentral.com)
We have now shown preclinical efficacy in multiple xenograft models with two differentiated CAR-NK cell therapies engineered with the functional attributes necessary for durable efficacy in solid tumors and manufactured using an efficient non-viral cell engineering system. (pipelinereview.com)
A single dose of engineered NK cells expressing CD70 CAR and secreted IL15 reduced tumor burden and increased survival in an RCC xenograft model, without the need for exogenous IL15. (pipelinereview.com)

In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)
Further, lung fibroblasts were cultured with and without cell supernatants from previously established cell cultures for 24 h. (medscimonit.com)
The exposure of lung fibroblasts to supernatant from mesothelial cell (with or without adenocarcinoma) did not affect collagen secretion. (medscimonit.com)
We use genetic, molecular, pharmacological tools to study how the KRAS oncogene maintain the tumorigenic state in lung, colorectal and pancreatic cancer cells. (cancer.gov)
We are developing scalable 3-dimensional (3D) culture models of tumor spheroid and lung organoid as an alternative and better approach to model KRAS mutant cancer in vitro . (cancer.gov)
The FORESEE Study is a multi-center, prospective clinical trial enrolling patients with Breast or Non-Small Cell Lung Cancer (NSCLC) who have suspicious or confirmed Leptomeningeal Metastases (LM). (stanford.edu)
Objective This study aimed to evaluate the effects of CIK cells on cisplatin (DDP) resistance in the human lung adenocarcinoma cell line A549/DDP. (jcancer.org)
The effect of CIK cells on re-sensitizing lung cancer cells to the chemotherapy drug was partially dependent on the secretion of IFN-γ. (jcancer.org)
PURPOSE: This study aimed to retrospectively evaluate the safety and feasibility of computed tomography (CT)-guided synchronous percutaneous core-needle biopsy (CNB) and microwave ablation (MWA) for stage I non-small cell lung cancer (NSCLC) in patients with idiopathic pulmonary fibrosis (IPF). (bvsalud.org)
For decades, most of the initial tumor biopsies have been obtained by bronchoscopy or computed tomography (CT)-guided transthoracic lung biopsy without concerning reports of cancer seeding following the latter. (bvsalud.org)
In this case report we discuss the patient history of a 56-year old women with low-differentiated squamous cell lung cancer who developed tumor seeding following a CT-guided transthoracic biopsy 11 months after the intervention. (bvsalud.org)

Concomitantly, new blood vessels are formed, inflammatory cells are recruited, and fibroblasts are activated in the surrounding tumor stroma. (dkfz.de)
Co-culture of tumor cells with fibroblasts in these 3D models enable the characterization of tumor-stroma interaction. (cancer.gov)
One of these cases caught Straussman's attention: human pancreatic and colorectal cancer cell lines could evade the chemotherapeutic drug gemcitabine when cultured with dermal fibroblasts. (the-scientist.com)
Then, he discovered that the fibroblasts he and his colleagues had been using contained bacteria called Mycoplasma , a common contaminant of tissue and cell cultures. (the-scientist.com)
In subsequent experiments, Straussman confirmed that it was the bacteria that were rescuing the cancer cells from chemotherapy: when he treated the fibroblasts with an antibiotic, they no longer protected cancer cells from gemcitabine, and when he infected other stromal cells with Mycoplasma , it endowed them with the ability to shield cancer cells from the drug. (the-scientist.com)
In contrast, non-tumoral BTN3A-expressing B cells, HUVEC and fibroblasts were unaffected. (bmj.com)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)

Data from a second CAR-NK cell therapy construct engineered using the TAILWIND™ platform are presented in a poster titled "Allogeneic Natural Killer Cells Engineered to Express HER2-directed CAR, Interleukin-15 and TGFβ Dominant Negative Receptor Effectively Control HER2+ Tumors. (pipelinereview.com)
CAT-179 demonstrated HER2-CAR-driven interferon gamma (IFNγ) production and tumor cell killing in vitro when co-cultured with HER2 + tumor cells. (pipelinereview.com)

However, the incubation of IL-17 was able to promote tumor cell migration and AKT, ERK and P38 phosphorylation in vitro . (austinpublishinggroup.com)

In line with this observation, elevated extracellular lactate concentrations up to 40 mM and corresponding low extracellular pH (pH e ) have been found in solid tumors under hypoxic conditions [ 18 - 22 ]. (hindawi.com)
Collectively, these preclinical data sets demonstrate the power of Catamaran's fully-integrated TAILWIND™ platform to deliver allogeneic NK cell therapies for solid tumors," said Vipin Suri, PhD, MBA, Chief Scientific Officer of Catamaran Bio. (pipelinereview.com)
These results demonstrate that NK cells engineered via the TAILWIND™ platform have a favorable pharmacologic profile and the potential for treatment of solid tumors. (pipelinereview.com)
Catamaran Bio is developing novel, off-the-shelf chimeric antigen receptor (CAR)-NK cell therapies designed to treat a broad range of cancers, including solid tumors. (pipelinereview.com)

We are using CRISPR gene editing and chemical biology approaches in these 3D models to discover non-oncogene addiction mechanism that are critically required for the anchorage-independent growth of cancer cells. (cancer.gov)
Created by COPASI 4.26 (Build 213) This model is described in the article: A mathematical model of antibody-dependent cellular cytotoxicity (ADCC) F. Hoffman, D. Gavaghan, J. Osborne, I.P. Barrett, T. You, H. Ghadially, R. Sainson, R.W. Wilkinson, H.M. Byrne Journal of Theoretical Biology 436 (2018) 39-50 Abstract: Immunotherapies exploit the immune system to target and kill cancer cells, while sparing healthy tis- sue. (ebi.ac.uk)
Everything that has to do with tumor biology can now be reexamined. (the-scientist.com)
Our proprietary capabilities enable us to harness the natural cancer-fighting properties of NK cells and enhance and tailor their effectiveness with the power of synthetic biology and innovative non-viral cell engineering. (pipelinereview.com)
Our team combines experienced biopharmaceutical leadership with founding scientists who are pioneers in NK cell biology, engineering, manufacturing and clinical application. (pipelinereview.com)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Microarrays were prepared in tumor biology. (lu.se)

CSCs display the slow-migratory, invadopod-rich phenotype that is the hallmark of disseminating tumor cells. (nature.com)
Furthermore, macrophages were shown to contribute to a niche that can support and maintain a breast CSC (BCSC) phenotype through heterotypic CD90/EphA4 signaling between macrophage and tumor cell 14 . (nature.com)
Cells undergoing EMT lose their epithelial characteristics and phenotype, acquire mesenchymal properties, and thus gain enhanced motility ( 12 ). (spandidos-publications.com)

The cellular cascade of molecular signals that instructs cells with fatally damaged DNA to self-destruct pivots on the p53 tumor suppressor gene. (news-medical.net)
When higher than normal levels of p53 tumor suppressor exist, there is enough p53 to bind to many regulatory sites in the cell's genome to activate the production of other proteins that will halt cell division if the DNA damage can be repaired. (news-medical.net)
Or, if the damage is too severe for the breaks to be repaired, critical backup protection, also governed by the p53 tumor suppressor protein, kicks in. (news-medical.net)
1) elucidating the role of neuroblastoma associated protein HIF-2a in neural crest development and its potential role in tumor onset, 2) how trunk neural crest specific genes can function as tumor suppressor genes in neuroblastoma, and 3) develop inducible tumor models using chick embryos. (lu.se)
Using 6 biphasic clear cell renal cell carcinoma cases we show that sarcomatoid clear cell renal cell carcinoma shares characteristic markers associated with loss of von Hippel-Lindau tumor suppressor with conventional clear cell renal cell carcinoma and also exhibits a markedly higher proliferative index. (lu.se)

The complex interactions between tumor cells and vessel, immune, or stroma cells are not yet traceable in an authentic manner in cell cultures in vitro. (dkfz.de)
Immunotherapies exploit the immune system to target and kill cancer cells, while sparing healthy tissue. (ebi.ac.uk)
In contrast, other studies suggest that IL-17 may protect against tumors, by promoting immune system-mediated tumor rejection [1,5,6]. (austinpublishinggroup.com)
Another study suggests that IL-17 may play an important role in the recruitment and infiltration of antitumor immune cells in early stages of bladder cancer [5]. (austinpublishinggroup.com)
The prognostic landscape of genes and infiltrating immune cells across human cancers. (bmj.com)
A key mechanism of immune evasion deployed by tumour cells is to reduce neoantigen presentation through down-regulation of the antigen presentation machinery. (portlandpress.com)
Although innate immune cells do not respond to specific antigenic epitopes on pathogens, they do produce restricted responses to par- ticular classes of pathogens through pattern-recognition receptors (PRR), such as Toll-like receptors (TLR) (18). (cdc.gov)

2,3 In the trial, 682 patients with complete tumor resection, with or without prior adjuvant chemotherapy, were randomly assigned to receive oral osimertinib at 80 mg once daily (n = 339) or placebo (n = 343) following recovery from surgery and standard adjuvant chemotherapy, if given. (ascopost.com)
Resection of these tumors is limited due to severe comorbidities that arise from surgical procedures. (bvsalud.org)

CNSide can detect and quantify tumor cells in the CSF from patients with Breast Cancer or NSCLC having a suspicious or confirmed LM. (stanford.edu)
Due to the difficulty in sampling and detection, few techniques exist to efficiently and consistently detect and quantify disseminated tumor cells (DTCs) in the bone marrow of cancer patients. (oncotarget.com)

In breast cancers from patients, the density of TMEM doorways correlates with the proportion of cancer cells expressing stem cell markers, indicating that in human breast cancer TMEM doorways are not only cancer cell intravasation portals but also CSC programming sites. (nature.com)
The sialyltransferase ST6GAL1 that adds α2-6 linked sialic acids to N-glycans of cell surface and secreted glycoproteins is prominently associated with many human cancers. (nature.com)
If p53 is inactivated, as it is in over half of all human cancers, checks and balances on cell growth fail to operate, and body cells start to accumulate mutations, which ultimately may lead to cancer. (news-medical.net)
CAMBRIDGE, MA, USA I November 10, 2022 I Catamaran Bio , Inc., a biotechnology company developing off-the-shelf NK cell therapies to treat cancer, today announced new data demonstrating preclinical efficacy of two CAR-NK cell therapies in solid and hematological cancers. (pipelinereview.com)
We use the most frequent human cancer - basal cell carcinoma - and one of the most common pediatric cancers - medulloblastoma - as the models for our studies. (fchampalimaud.org)
Assessment of tumor-infiltrating TCRVγ9Vδ2 γδ lymphocyte abundance by deconvolution of human cancers microarrays. (bmj.com)

Using a mouse model of ependymoma-a chemoresistant brain tumor-we combined multicell high-throughput screening (HTS), kinome-wide binding assays, and in vivo efficacy studies, to identify potential treatments with predicted toxicity against neural stem cells (NSC). (nih.gov)
SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)
Distinct hallmarks of cancer were examined using in silico analysis, various in vitro cell culture assays, and ex vivo organotypic rat brain slice culture model. (hindawi.com)
We investigated the impact on tumor malignancy in F98 glioma cells using in silico analysis, in vitro cell culture assays, and ex vivo vascular organotypic glioma impact model (VOGIM) [ 35 ] by implanting F98 cells into rat brain slices. (hindawi.com)
We use these results to explain what information about the tumour cell kill rate can be extracted from the cytotoxicity assays. (ebi.ac.uk)

Engineering of IL15 in CAT-179 significantly enhanced NK cell persistence and in vitro and in vivo and eliminated the need for exogenous cytokine support. (pipelinereview.com)
Background Cytokine-induced killer (CIK) cells can potentially enhance the tumor-killing activity of chemotherapy. (jcancer.org)
Methods The detect resistance index, drug resistance related-genes and cytokine secretion of A549/DDP co-cultured with CIK cells were assayed in vitro . (jcancer.org)

Since the p53 protein is able to trigger such drastic action as cellular suicide, the cells of the body must ensure that the p53 protein is only activated when damage is sensed and that the protein is quickly degraded when it is not needed. (news-medical.net)
Our preclinical studies demonstrate that Triphala is effective in inhibiting the growth of human pancreatic cancer cells in both cellular and in vivo model. (biomedcentral.com)
As a consequence, such changes disturb cellular signaling pathways and regulatory circuits in affected cells conferring growth advantages, landscaping the micro-environment of the tumor cells, and supporting escape from immunosurveillance. (dkfz.de)
Preclinical assessment of molecules that may cause antibody-dependent cellular cytotoxicity (ADCC) involves co-culturing cancer cells, NK cells and antibody in vitro for several hours and measuring subsequent levels of tumour cell lysis. (ebi.ac.uk)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The factors and cellular processes required for the induction of dormancy and subsequent tumor cell reactivation remain largely undefined [ 6 ]. (oncotarget.com)
Cell type and tissue specific alterations in fine GAG structure, which are strictly predetermined [ 8 - 10 ], allow these molecules to modulate with high specificity different cellular processes [ 7 ]. (hindawi.com)
Key implication of CD277/butyrophilin-3 (BTN3A) in cellular stress sensing by a major human γδ T-cell subset. (bmj.com)
In addition, we identify cellular mechanisms that potentially precondition tumors to resist therapy. (bvsalud.org)

Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. (mdpi.com)
Background gdT-cells are innate-like lymphocytes described as potent killer of cancer cells whose infiltration into tumors is associated with a positive prognosis. (bmj.com)
In lymph nodes, the tumor cells infiltrate the interfollicular areas and medullary areas, often diffusely, whereas bone marrow evaluation may show variable degrees of infiltration ranging from sparse to significant. (medscape.com)

These cells consist of lymphocytes (T cells, B cells, NK cells) and monocytes, whereas erythrocytes and platelets have no nuclei, and granulocytes (neutrophils, basophils, and eosinophils) have multi-lobed nuclei. (wikipedia.org)
In humans, lymphocytes make up the majority of the PBMC population, followed by monocytes, and only a small percentage of dendritic cells. (wikipedia.org)
Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

Growth-inhibitory effects of Triphala were evaluated in Capan-2, BxPC-3 and HPDE-6 cells by Sulphoradamine-B assay. (biomedcentral.com)
Apoptosis was determined by cell death assay and western blotting. (biomedcentral.com)
Here we develop a mathematical model of such an in vitro ADCC assay, formulated as a system of time-dependent ordinary differential equations and in which NK cells kill cancer cells at a rate which depends on the amount of antibody bound to each cancer cell. (ebi.ac.uk)
Cell migration capacity and intracellular signaling pathways activated by IL-17 were assessed by wound healing assay and flow cytometry, respectively. (austinpublishinggroup.com)

The range of tumor models will be adjusted to changing scientific needs. (dkfz.de)
ICT01-activated g9d2 T-cells continuously and serially kill a wide range of tumor cells in multi-day co-culture conditions. (bmj.com)

Aggressive breast cancer is a highly heterogeneous disease caused by a variety of distinct cell-intrinsic genetic alterations in mammary epithelial cells, leading to vastly heterogenic disease manifestation in individual patients and predominantly affecting patient prognosis and treatment options [ 14 ]. (nature.com)
The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)
Together, our findings suggest a promising role for P-Tex cells in the prognosis of patients with HPV-positive HNSCC by providing modest but persistent anti-tumor effects. (elifesciences.org)

In mammary gland development, Notch-dependent heterotypic signaling between resident tissue macrophages and mammary stem cells supports survival and function of the normal mammary stem cell 20 . (nature.com)
Not surprisingly, the regulation of this vital safeguard has been studied in great detail for many years but mainly in tissue culture, or in vitro, models. (news-medical.net)
Previous tissue culture studies by several labs around the world indicated that tinkering with the tail end prevented the protein from being flagged for degradation or activation. (news-medical.net)
We are studying components of blood, tumor tissue, normal tissue, and other fluids, such as urine, cerebrospinal fluid, abdominal or chest fluid in patients with cancer. (stanford.edu)
The compelling conclusion that proliferative exhausted T cells are associated with response in HPV+ head and neck cancer is supported by the cohort of 14 patients with paired tumor and adjacent normal tissue and rigorous bioinformatic analysis of nearly 50,000 single CD3+ T cell transcriptomes. (elifesciences.org)
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)
Patients had to have resectable tumors (stage IB to IIIA) and predominantly nonsquamous histology and EGFR exon 19 deletions or exon 21 L858R mutations identified prospectively from tumor tissue in a central laboratory by the cobas EGFR Mutation Test. (ascopost.com)
Cells grown in vitro from neoplastic tissue. (bvsalud.org)

Besides, upregulated MCT4 has been linked to altered tumor metabolism as well as to increased growth and survival in breast and pancreatic cancer [ 33 , 34 ]. (hindawi.com)
However, there has only been one previous functional study investigating MCT4 in glioblastoma (GBM) neurospheres, mainly focusing on tumor growth and survival dependent on the oxygen level [ 14 ]. (hindawi.com)
In some cases, neuron survival was confirmed by measuring subsequent neurite growth in monolayer culture. (open.ac.uk)
Exposure of Capan-2 cells to the aqueous extract of Triphala for 24 h resulted in the significant decrease in the survival of cells in a dose-dependent manner with an IC50 of about 50 μg/ml. (biomedcentral.com)
Triphala-mediated reduced cell survival correlated with induction of apoptosis, which was associated with reactive oxygen species (ROS) generation. (biomedcentral.com)
Research from his laboratory has identified multiple stress-response pathways that are critical for the survival of cancer cells. (cancer.gov)
Our work has demonstrated that KRAS mutant cells exhibit non-oncogene addiction to multiple stress-response pathways that maintain chromosome stability, mRNA splicing, protein SUMOylation and autophagy for survival. (cancer.gov)
A proliferative exhausted CD8 + T cell cluster (P-Tex) which was beneficial to survival outcomes of patients with HPV-positive HNSCC was identified. (elifesciences.org)
Recently, researches on this field point out the idea that IL-17 promotes tumor growth, especially through the activation of IL-6- STAT3 cascade, an oncogenic transcription factor that up regulates pro-survival and pro-angiogenic genes [1-4]. (austinpublishinggroup.com)
In mouse AML and ovarian cancer models, repeated injections of ICT01 delayed tumor growth and significantly prolonged animal survival. (bmj.com)
Insulin receptor substrate 1 (IRS-1) is an adaptor protein that integrates multiple transmembrane signals from growth factors and hormones, to regulate cell growth, survival, differentiation and metabolism ( 4 ). (spandidos-publications.com)
Tumour-specific expression of MHC-I and MHC-II has been associated with improved patient survival in most clinical studies. (portlandpress.com)

On the other hand, Triphala failed to induce apoptosis or activate ERK or p53 in normal human pancreatic ductal epithelial (HPDE-6) cells. (biomedcentral.com)
Our data show, for the first time, the ability of IL-17RA/IL-17 to induce migration of human tumor cell lines in vitro , possibly via activation of MAP kinases and AKT. (austinpublishinggroup.com)
It may also invade and proliferate within heart and coronary artery endothelial cells, and, along with Streptococcus sanguis, it may also induce platelet aggregation associated with thrombus formation. (medscape.com)

Subsequently, PBMCs are challenged with tumor antigens such as tumor stem cell antigen. (wikipedia.org)
1 Prostate-specific antigen is a protein produced by the cells of the prostate gland, including both cancerous cells as well as cells that are benign. (lifeextension.com)
P-Tex cells could aggregate in the antigen-presenting cell niches and activate certain signaling pathways. (elifesciences.org)
Their expression in tumour cells is modulated by a complex interplay of genomic, transcriptomic and post translational factors involving multiple intracellular antigen processing pathways. (portlandpress.com)
The neoplastic cells in blastic plasmacytoid dendritic cell neoplasm (BPDCN) are typically positive for CD45, HLA-DR, CD43, CD4, CD56, and cutaneous lymphocyte-associated antigen (CLA). (medscape.com)
The pDC markers, CD123 (strong), TCL-1 (90% of cases), CD45RA, blood dendritic cell antigen (BCDA)-2 (expressed in more mature cases), and myxovirus A (MxA) may be detected by flow cytometric immunophenotyping (FCI) and/or immunohistochemical (IHC) staining. (medscape.com)

Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a malignancy derived from plasmacyoid dendritic cells. (medscape.com)
[ 1 ] Within the 2016 World Health Organization (WHO) category of "acute myeloid leukemia and related neoplasms," the related neoplasms derive from immature cells with evidence of myeloid differentiation, or from precursors of plasmacytoid dendritic cells. (medscape.com)
The precursors of plasmacytoid dendritic cells are the cells of origin for blastic plasmacytoid dendritic cell neoplasm (BPDCN), a clinically aggressive disease with a proclivity for the skin and leukemic involvement and for which no consensus currently exists regarding optimal treatment modalities. (medscape.com)

When assayed in human and cynomolgus PBMCs in vitro, ICT01 promoted a robust and specific activation of g9d2 T-cells as shown by concentration dependent increase in cell surface CD69 and CD25 and cytokines secretion (IFNγ, TNFα). (bmj.com)
The cytokines tumor necrosis factor, interleukin-1, and gamma interferon, which have been implicated in the pathogenesis of cerebral malaria and are known to promote intercellular adhesion in other systems, did not enhance the cytoadherence of P. falciparum isolates to C32 melanoma cells. (tropmedres.ac)
Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)
1 ]. Interleukinc8 (ILc8) and tumour necroc ing PCs prepared from apheresis of single sis factor alfa (TNF-) are some of the donors or from pooled samples, is not still cytokines responsible for NHFR [ 2 ]. (who.int)

After co-culture with tumour-associated pancreatic stellate cells (TPSCs), pancreatic cancer cells displayed up to 8-fold reduction in levels of epithelial-mesenchymal transition (EMT) markers E-cadherin and ZO-1, while β-catenin and vimentin levels were increased. (lu.se)
In summary, co-culture with TPSCs induced molecular changes associated with EMT in pancreatic cancer cells, regardless of differentiation status, and the organotypic model demonstrated the influence of microenvironmental factors, such as glucose, stellate cells and macrophages, on pancreatic cancer cell invasion. (lu.se)

Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion and resistance to cell stress and chemo- and radio-treatments. (nature.com)

We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)
Interferons have been shown to be potential anti-cancer agents and to inhibit tumor cell growth in culture. (nih.gov)
This brain tumor type is highly proliferative and shows an infiltrative growth pattern, accounting for the high recurrence rates in patients. (hindawi.com)
Although 2-dimensional cell culture has traditionally been a useful method to study cancer cell behavior, this model does not capture the anchorage-independent nature of cancer cell growth. (cancer.gov)
Because tumor growth is essentially exponential, with one cell dividing into two, two to four, four to eight, and so on, a tumor cell product such as PSA can reflect such exponential growth-measuring the time it takes for PSA to double (PSA doubling time, or PSADT). (lifeextension.com)
In phytochemistry, plant saponins are well known to have a wide spectrum of activities including lowering blood cholesterol, inhibiting cancer cell growth, and acting as antifungal and antibacterial agents. (shamansgarden.com)
Free glycosaminoglycans (GAGs) and proteoglycan- (PG-) containing GAGs, key effectors of cell surface, pericellular and extracellular microenvironments, perform multiple functions in cancer by virtue of their coded structure and their ability to interact with both ligands and receptors that regulate cancer growth [ 1 - 4 ]. (hindawi.com)
Their properties and ability to support normal growth of cancer cells were evaluated. (edu.au)
UCN-01, a protein kinase C (PKC) inhibitor, induces growth arrest and apoptosis in cancer cells and was recently introduced in a phase I clinical trial. (karger.com)
We report that, in addition to direct inhibitory effects on endothelial cell growth, UCN-01 abrogates hypoxia-mediated transactivation of HIF-1-responsive promoters in a prostate cancer cell line. (karger.com)
We further selected a representative case, clinically demonstrating direct overgrowth of the sarcomatoid component into the liver and colon, for extended immunohistochemical characterization, resulting in a further set of positive and negative epithelial-mesenchymal transition markers as well as pronounced transforming growth factor β positivity, indicating that sarcomatoid clear cell renal cell carcinoma may be associated to epithelial-mesenchymal transition. (lu.se)
Transforming growth factor β1 exposure of in vitro cultured primary clear cell renal cell carcinoma cells resulted in cells adopting a mesenchymal morphology similar to sarcomatoid clear cell renal cell carcinoma. (lu.se)
We therefore suggest that sarcomatoid clear cell renal cell carcinoma morphologically and immunohistochemically may represent a completed epithelial-mesenchymal transition and that transforming growth factor β1 could be an important driving force during the sarcomatoid transdifferentiation of clear cell renal cell carcinoma. (lu.se)

[ 4 , 5 ] Previously used terms for this disorder have included CD4+CD56+ hematodermic tumor and blastic natural killer (NK) cell lymphoma. (medscape.com)

In summary, our data highlight MCT4 /SLC16A3 as a key gene for distinct hallmarks of tumor malignancy in glioma cells. (hindawi.com)

PBMCs from cancer patients can be extracted and cultured in vitro. (wikipedia.org)
3 ImCheck Therapeutics is developing ICT01, a humanized anti-BTN3A (IgG1, Fc-silenced), g9d2 T-cell-activating antibody for the treatment of patients with solid or hematologic tumors. (bmj.com)
To determine whether isolates of Plasmodium falciparum have intrinsically different cytoadherent properties and whether these differences contribute to the clinical severity of human falciparum malaria, we studied the cytoadherence to C32 melanoma cells in vitro of 59 parasite isolates from patients with naturally acquired infections in Thailand. (tropmedres.ac)
In vitro cytoadherence was significantly greater for isolates from patients with biochemical evidence of severe malaria. (tropmedres.ac)
[ 11 ] Although BPDCN occurs more frequently in the elderly, the tumor may occur in patients of any age, including pediatric patients. (medscape.com)
We recommend meloxicam for patients with extraabdominal desmoid tumors, instead of surgery first," said lead author Yoshihiro Nishida, MD, PhD, from the Department of Orthopedic Surgery at Nagoya University Graduate School of Medicine in Japan. (medscape.com)
In this study, between 1991 and 2003, Dr. Nishida and colleagues identified 30 patients who were surgically treated for extraabdominal desmoid tumors at their institutions (excluding those with intraabdominal, familial adenomatous polyposis-associated, and unresectable tumors). (medscape.com)
However, the significant morbidity of aggressive chemotherapy should be avoided in patients with extraabdominal desmoids tumors," they say. (medscape.com)

In cultured cells and animal tumor implantation models, osimertinib exhibited antitumor activity against NSCLC lines with EGFR mutations (T790M/L858R, L858R, T790M/exon 19 deletion, and exon 19 deletion) and, to a lesser extent, those with wild-type EGFR amplifications. (ascopost.com)

Our observations suggest that the tumor paracrine effect affects biological pathways of pleurodesis. (medscimonit.com)

In esophageal squamous cell carcinoma (ESCC), IL-17 led to the production of inflammatory chemokines (CXCL9, CXCL10 and CCL2, CCL20), which are associated with the migration of T cells, NK cells, and DCs, respectively. (austinpublishinggroup.com)
OE-21 human esophageal squamous cell carcinoma was a gift from INCA (Rio de Janeiro, Brazil). (austinpublishinggroup.com)
In order to find optimal parameters for specific targeting of tumour cells, we compared normal human epidermal keratinocytes with a human squamous skin cancer cell line. (chalmers.se)

It initiates the process of programmed cell death, or apoptosis, which directs the cell to commit suicide, permanently removing the damaged DNA from the organism. (news-medical.net)
Triphala-induced apoptosis was linked with phosphorylation of p53 at Ser-15 and ERK at Thr-202/Tyr-204 in Capan-2 cells. (biomedcentral.com)
Pretreatment of cells with pifithrin-α or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. (biomedcentral.com)
Similarly, Triphala induced apoptosis in another pancreatic cancer cell line BxPC-3 by activating ERK. (biomedcentral.com)

These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation, which will separate the blood into a top layer of plasma, followed by a layer of PBMCs (buffy coat) and a bottom fraction of polymorphonuclear cells (such as neutrophils and eosinophils) and erythrocytes. (wikipedia.org)
Indeed, footprints of JC polyomavirus and Merkel cell polyomavirus have been detected in PBMCs from pregnant women and women affected by spontaneous abortion. (wikipedia.org)
ICT01 effects on human and cynomolgus PBMCs were characterized in vitro using flow cytometry. (bmj.com)

Above mentioned effects were significantly blocked when the cells were pretreated with an antioxidant N-acetylcysteine (NAC), suggesting the involvement of ROS generation. (biomedcentral.com)
The study indicates significantly enhanced cell death in the presence of nanoparticles and important differences in treatment efficiency between normal and tumour cells. (chalmers.se)
Results After A549/DDP co-culture with CIK cells, the DDP resistance of A549/DDP significantly decreased in a time-dependent manner. (jcancer.org)
The secretion of interferon (IFN)- γ significantly increased along with the co-culture time of A549/DDP with CIK cells. (jcancer.org)

Numerical simulations generated using experimentally-based parameter estimates reveal that the system evolves on two timescales: a fast timescale on which antibodies bind to receptors on the surface of the tumour cells, and NK cells form complexes with the cancer cells, and a longer time-scale on which the NK cells kill the cancer cells. (ebi.ac.uk)
However, there was no correlation between the degree of in vitro cytoadherence and cerebral symptoms, which suggests that other receptors and/or host factors may be important in the adherence of malaria parasites to cerebral vascular endothelium. (tropmedres.ac)

Methods: Fluorescence microscopy confirmed that mTHPC was incorporated into all three cell types. (open.ac.uk)
We are using our TAILWIND™ platform , an integrated suite of technologies, to specifically address the end-to-end methods of engineering, processing and manufacturing NK cells and rapidly advance our pipeline of CAR-NK cell therapy programs. (pipelinereview.com)
Such progress provides us now with new opportunities to consider alternative approaches to sensitization testing, including the design of in vitro test methods. (cdc.gov)
Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)
1999), there has been little investment or progress in based solely on in vitro methods. (cdc.gov)

Stable F98 glioma cell lines with MCT4 overexpression, normal expression, and knockdown were generated. (hindawi.com)
Furthermore, we found that knockdown of IRS-1 expression enhanced cell invasive potency and induced EMT in parallel with upregulation of miR-9 expression. (spandidos-publications.com)

Surgery remains the mainstay for resectable sporadic desmoid tumors, especially extraabdominal ones, the authors write, and there is growing evidence that chemotherapy might be effective in managing life-threatening intraabdominal desmoid tumors. (medscape.com)

The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)
The impact of this non-canonical extrinsic mechanism of ST6GAL1 on tumor cell pathobiology is not known. (nature.com)
however, in vitro a primary mechanism of cytotoxicity is through the depletion of tryptophan. (nih.gov)
We are particularly focused on dissecting mechanism of non-oncogene addiction in KRAS mutant cells that plays a critical role in alleviating oncogenic stress. (cancer.gov)
2 If PSA readings begin to elevate, there are interventions that can reduce or stabilize the production of PSA, shutting down a mechanism used by cancer cells to escape their confinement within the prostate gland. (lifeextension.com)

abstract = "Three-dimensional (3D) cells in vitro culture are becoming increasingly popular in cancer research because some important signals are lost when cells are cultured in a two-dimensional (2D) substrate. (edu.au)

Mechanistically, stemness is induced in non-stem cancer cells upon their direct contact with macrophages via Notch-Jagged signaling. (nature.com)
We were among the first to carry out synthetic lethal screens to identify non-oncogene addiction in KRAS mutant cancer cells. (cancer.gov)
A s a postdoc at the Broad Institute of MIT and Harvard in 2008, cancer biologist Ravid Straussman worked on a collaborative effort to screen normal cells for their influence on cancer cells' responses to various anticancer therapies. (the-scientist.com)
In the lab, the cancer cells "continue to proliferate under therapy," Straussman explains. (the-scientist.com)
how stromal cells can protect cancer cells from chemotherapy. (the-scientist.com)
So we know for a fact it wasn't the stromal cells that rescued the cancer cells from chemotherapy, but actually it was the Mycoplasma that did that. (the-scientist.com)
We never found Mycoplasma in pancreatic cancer, but we found many other bacteria," says Straussman, "and we found that [when] we isolated these bacteria from tumors, they can also rescue cancer cells from chemotherapy" in vitro. (the-scientist.com)
Further research showed that bacteria lived inside the cancer cells near the nuclear envelope. (the-scientist.com)
Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. (elifesciences.org)
It is the result of the dissemination of cancer cells to the bone marrow. (oncotarget.com)
Since hypoxia activates hypoxia-inducible factor (HIF-1)-dependent transcription in cancer cells that, in a paracrine fashion, drive tumor angiogenesis, we investigated the effects of UCN-01 on HIF-1-responsive promoter constructs. (karger.com)
arations verified the presence of cancer cells in all samples. (lu.se)

Finally, MCT4 inhibition mitigates the induction of angiogenesis, suggesting that MCT4 also plays a crucial role in tumor-related angiogenesis. (hindawi.com)

We aimed to evaluate the impact of cancer cell paracrine on mesothelial cell response to different sclerosing agents. (medscimonit.com)

The project turned up hundreds of examples of stromal cells-best known for forming the connective tissues that support organs-somehow conferring drug resistance on their cancerous neighbors in vitro. (the-scientist.com)
Contrary to the common dogma at the time that tumors were sterile, Straussman says he wondered if the cancerous growths could in fact be hosting microbial life, like so many tissues in the human body that modern genomic tools had revealed to harbor their own microbiomes . (the-scientist.com)
Tissues and Cells. (lu.se)

Moreover, UCN-01, at concentrations as low as 32 n M, prevent microvessel outgrowth from explant cultures of rat aortic rings. (karger.com)

Sarcomatoid conversion of clear cell renal cell carcinoma in relation to epithelial-to-mesenchymal transition. (lu.se)
Approximately 8% of clear cell renal cell carcinoma cases contain regions of radically different morphology, demonstrating a mesenchymal appearance histologically resembling sarcomas. (lu.se)
These biphasic neoplasms are called sarcomatoid clear cell renal cell carcinoma. (lu.se)
In the present study we investigate whether the sarcomatoid conversion of clear cell renal cell carcinoma could be interpreted as linked to the process of epithelial-mesenchymal transition. (lu.se)
Furthermore the sarcomatoid elements demonstrate an enhanced expression of epithelial-mesenchymal transition related mesenchymal markers as compared with the clear cell renal cell carcinoma counterparts. (lu.se)

Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)

Antibody therapies, an important class of immunotherapies, involve the binding to specific antigens on the surface of the tumour cells of antibodies that activate natural killer (NK) cells to kill the tu- mour cells. (ebi.ac.uk)

However, this finding has triggered further interest in utilising tumour-specific MHC-II to harness sustained anti-tumour immunity through the activation of CD4 + T helper cells. (portlandpress.com)
Thus, their reactivation represents an attractive way to unleash anti-tumour immunity. (portlandpress.com)

90% of cancer-related deaths, is a multi-step process involving cancer cell intravasation into the bloodstream, dissemination to distant sites, and seeding in new organs 1 . (nature.com)

At the apex of the hierarchy are the cancer stem cells (CSCs). (nature.com)
DTCs occupy the hematopoietic stem cell niche within the bone marrow [ 5 ], often remaining dormant for years before becoming reactivated, leading to cancer recurrence [ 6 ]. (oncotarget.com)
We focus on the childhood tumor form neuroblastoma, originating from cells of the sympathetic nervous system (SNS) in turn derived from the transient neural crest stem cell population. (lu.se)

Anatomy21

Organisms11

Diseases79

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes49

Disciplines and Occupations1

Information Science4

Health Care6

Progression12

Microenvironment8

Metastasis11

Primary tumors2

Adjuvant therapy after tumor resection3

Metastatic6

Proliferation14

Cytotoxic5

Vivo and in vitro2

Malignant6

Mice9

Xenograft5

Lung11

Fibroblasts7

HER22

Promote tumor cell1

Solid tumors4

Biology7

Phenotype3

Suppressor5

Immune7

Resection2

Detect and quantify2

Cancers6

Assays5

Cytokine3

Cellular9

Infiltration3

Lymphocytes3

Assay4

Range of tumor2

Prognosis3

Tissue8

Survival12

Induce3

Antigen6

Dendritic cells3

Cytokines4

Pancreatic cancer cells2

Promotes tumor1

Growth13

Blastic natural killer1

Malignancy1

Patients8

NSCLC1

Pathways1

Squamous3

Apoptosis4

PBMCs3

Significantly4

Receptors2

Methods5

Knockdown2

Resectable1

Mechanism5

Abstract1

Cancer cells12

Angiogenesis1

Paracrine1

Tissues3

Concentrations1

Renal cell carc5

Genes1

Antigens1

Immunity2

Intravasation1

Stem3