Vitro rosette formation. Medical search. Frequent questions

With the formation of neural rosettes, neural tube development and neural differentiation can be modeled using human pluripotent stem cells (hPSCs) in vitro . (creative-biolabs.com)
Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation. (creative-biolabs.com)
Machine learning-assisted high-content analysis of pluripotent stem cell-derived embryos in vitro. (caltech.edu)

didemnin b (db) is a cyclic peptide with potent immunosuppressive activity in vitro and in the murine graft-versus-host-reaction (gvhr), the only measure of in vivo immunity tested in our prior studies. (liverpool.ac.uk)
However, combined MYC overexpression and SMARCA4 loss successfully induced tumor formation in vivo after orthotopic transplantation in recipient mice. (bvsalud.org)
Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
People with HSV antibody (Ab) can regularly be shown to harbor a latent virus of either type in an appropriate ganglion, and that the virus may be activated by many dissimilar stimuli, both in vivo 1 and in vitro . (webmedcentral.co.uk)
Using an ex vivo airway culture model, we show that ablation of PC does not perturb deuterosome formation and centriole amplification. (elifesciences.org)
Quantification of centriole abundance in vitro and in vivo identified a linear relationship between surface area and centriole number. (elifesciences.org)

Polymorphonuclear neutrophils (PMNs) activated in vitro may express molecules normally associated with antigen presenting cells (APCs). (annexpublishers.co)
PMNs activated in vitro may express molecules normally associated with antigen presenting cells required for T cell activation. (annexpublishers.co)
In contrast to normal T -cells, neoplastic mature T -cells respond directly to TCGF, requiring no prior lectin or antigen in vitro activation. (science-connections.com)

Cytoadherence characteristics of Plasmodium falciparum isolates in Thailand using an in vitro human lung endothelial cells model. (ajtmh.org)
Using an in vitro model of human lung endothelial cells, we studied different characteristics of Plasmodium falciparum isolates as potential factors for malaria severity in 2 Thai patient groups: 27 with complicated malaria and 42 with uncomplicated malaria. (ajtmh.org)
In vitro work aims at a full characterization of the molecular composition of podosomes and elucidation of the molecular mechanisms involved in their assembly and disassembly in both microvascular and macrovascular endothelial cells. (u-bordeaux.fr)
Endothelial podosome rosettes in cultured endothelial cells. (u-bordeaux.fr)

The production of progesterone at this time induces embryroid body (akin to blastulation) and rosette (akin to neurulation) formation in vitro. (wikipedia.org)
Suppression of P4 signaling following withdrawal of progesterone, or treatment with the progesterone receptor antagonist RU-486 (mifepristone), inhibits the differentiation of hESC colonies into embryoid bodies (blastulation) or rosettes (neurulation). (wikipedia.org)
Besides, the mechanism of neural rosettes formation relates to secondary neurulation rather than that of primary neurulation. (creative-biolabs.com)

And inhibition of BMP signaling would result in the expression of these markers and the rosette morphology. (creative-biolabs.com)
A six-fold assembly of the trimeric computational oligomer had the lowest potential energy per monomer and was consistent with rosette CSC morphology. (osti.gov)

Here we highlight the journey towards recapitulating human brain development and disease in a dish, progressing from two‐dimensional in vitro systems to the third dimension provided by brain organoids. (ehu.eus)

Fig.1 Cell morphological changes during rosette formation. (creative-biolabs.com)
Calcium signaling mediates five types of cell morphological changes to form neural rosettes. (creative-biolabs.com)
The mechanism of NET formation implicate morphological changes of PMN: after activation, the cells flatten and they attach to the substratum, the nucleus loses the lobules and the chromatin decondenses Nuclear membranes progressively lose their integrity. (annexpublishers.co)

An in vitro model of early human embryogenesis (human embryonic stem cells (hESCs)) has demonstrated that hCG promotes cell proliferation via the LH/hCG receptor (LHCGR). (wikipedia.org)
Estrogens and progesterone promote mammary epithelial cell proliferation resulting in the formation of the primary and secondary ductal structure. (wikipedia.org)
Both alterations did not increase proliferation of granule cell precursors in vitro. (bvsalud.org)
Effect of targeted erythrocytes coated with recombinant human interleukin 2 on T-lymphocyte proliferation in vitro. (tamu.edu)

We are presently exploring the contribution of these molecules to podosome formation and functioning. (u-bordeaux.fr)
3,4 Previous studies have described the involvement of several adhesion molecules in HL rosette formation. (ashpublications.org)

Fig.2 Involvement of active BMP signaling in differentiation and formation of neural rosettes. (creative-biolabs.com)
The hPSCs-based neural rosettes have been widely used as models for the research of neural tube development as well as neural differentiation in vitro . (creative-biolabs.com)

early, quick release (5 to 60 minutes after in vitro stimulation with Staphylococcus aureus or lipopolysaccharide (LPS) and 2. (annexpublishers.co)

Here we have taken parasite isolates from 150 patients with defined clinical syndromes as well as isolates from 50 healthy but parasitized community controls and quantitatively assessed their binding to purified endothelial receptors in vitro. (ox.ac.uk)
Because rosette formation by uninfected cells has also been a phenotype associated with disease severity and one that may interfere in vitro with receptor binding, we also assessed rosette formation in all isolates. (ox.ac.uk)

Progesterone induces formation of tertiary side-branches in the mammary glands during puberty and during the luteal phase of the menstrual cycle upon which lobuloalveolar structures form under the influence of prolactin. (wikipedia.org)
Neural rosettes refer to radially organized cellular structures. (creative-biolabs.com)
Investigating the cellular architecture of the developing cortex, we observed a loss of the radial orientation of radial glial cells, likely due to the disruption of the apical band of adherens junctions, which is the first effect observed after loss of the protein, and the consequent formation of rosette like structures in the brain parenchyma. (uni-muenchen.de)
TGFß-treated cells exhibit ring-like structures (podosome rosettes, right panel), where vinculin surrounds F-actin cores (tbottom panel: higher magnification of the boxed areasl). (u-bordeaux.fr)

During embryonic development, there are a series of morphogenetic events that lead to the formation of the neural tube. (creative-biolabs.com)
The neural markers expressed in neural rosettes include SOX2, PAX6, and SOX1. (creative-biolabs.com)
In addition, neural rosettes can differentiate into distinct region-specific neurons and glia. (creative-biolabs.com)
Recent research has shown that the dismantlement of neural rosettes promotes neurogenesis and astrogenesis prematurely, which means that the complete rosette structure is essential for orderly neurodevelopment. (creative-biolabs.com)

Using a GFP-tagged bri1-120 construct, we detected the bri1-120 protein in the plasma membrane, and showed that the phenotypic defects in the rosette leaves of bri1-301 , a kinase-inactive weak allele of BRI1 , can be restored by the overexpression of the bri1-120 proteins in bri1-301 . (biomedcentral.com)

The resulting transgenic lines displayed more secondary cauline-leaf branches and primary and secondary rosette-leaf branches. (rwth-aachen.de)

Recombinant ACBPs have been tested and shown to bind acyl-CoA esters in vitro using binding assays such as the Lipidex assay. (aocs.org)
An in-vitro ubiquitination assay showed that SAD1 can be ubiquitinylated by RGLG1 and RGLG2 of both A. thaliana and Zea mays in the presence of the E2 ubiquitin-conjugating enzymes UbcH5b and UbcH13. (rwth-aachen.de)

Cytoadherence was significantly associated with malaria severity (P = 0.05) in contrast to rosette formation (P = 0.9). (ajtmh.org)

We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
Two well-defined pathological hallmarks of AD have been described: the formation of extracellular amyloid plaques and the development of intracellular neurofibrillary tangles (NFTs) formed by aggregated hyperphosphorylated TAU protein [ 4 ]. (biomedcentral.com)

Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)

In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. (biomedcentral.com)

My current focus is to develop an in vitro human brain model of Gauchers disease, the most common form of lysosomal storage disorder. (lu.se)
The rosette formation is a histopathological hallmark for multiple tumors, such as a primitive neuroectodermal tumor, human medulloblastoma, glioblastoma, and neuroblastoma. (creative-biolabs.com)
An in vitro stem cell model of human epiblast and yolk sac interaction. (caltech.edu)
In tubes several parameters affecting the colony formation of mouse and human granulocytes and macrophages as well as of human peripheral T and B lymphocyte from mouse spleen were optimized. (karger.com)

In this study the highest level of rosette-forming parasites was found in the anemic group and not the cerebral malaria group. (ox.ac.uk)

Intriguingly, the level of CD58 expression correlated with the extent of rosette formation, and CD58 knockout or CD2 blockade reduced both rosette formation and T-cell activation. (ashpublications.org)

form characteristic sulfur granules in infected tissue but not in vitro. (pediagenosis.com)

The phenotype is characterized by changes in the formation of the cortical layers. (uni-muenchen.de)

The formation of NETs with sterile inflammatory stimuli from crystals of monosodium urate (MSU) has been of great importance in patients with gout [ 6 ]. (annexpublishers.co)
Hence, in our initial attempts to develop cell lines from patients with T -cell neoplasias, we chose clinical subpopulations which represent a more mature form of disease, namely, the cutaneous T -cell lymphomas and leukemias and E-rosette positive T -cell ALL (Gupta and Good 1980). (science-connections.com)

These roles have been mostly established in vitro using dominant negative or constitutively active constructs. (uni-muenchen.de)
Examination of these cells showed that over 90% were E-rosette positive and that all were karyotypically normal and negative for terminal transferase, EBV, and surface immunoglobulins (Morgan et al. (science-connections.com)

All of these functions are of importance during the formation of the cerebral neocortex, which consists of the generation of its different cell types, their migration to their destination and their maturation to a functional network. (uni-muenchen.de)

In conclusion, the multifaceted data support a rosette CSC with 18 CESAs that mediates the synthesis of a fundamental microfibril composed of 18 glucan chains. (osti.gov)
In conclusion, T-cell rosetting in HL is established by formation of the IS, and activation of rosetting T cells critically depends on the interaction of both TCR-HLA-II and CD2-CD58. (ashpublications.org)

Symptoms include stunted growth, the formation of spores in the tassel and the cob, phyllody in the male and female organs, and formation of subapical ears caused by suppression of apical dominance of the maize ear. (rwth-aachen.de)

In regard to binding properties, no association existed between cytoadherence and rosette phenotypes (P = 0.1) and hypothrombocytemia increased the cytoadherence level (P = 0.007). (ajtmh.org)

This configuration of the uterine structure has been called a rosette. (med-chem.com)

RBC were covalently coupled to specific monoclonal antibody (mAb) towards the lymphocyte cell-surface marker Thyl.2, coated with rIL 2, subsequently exposed to the target CTLL cells, then evaluated for CTLL/RBC rosette formation. (tamu.edu)
Given that recombinant Arabidopsis ACBPs show preferences for different acyl-CoA esters and phospholipids in in vitro binding assays, it was hypothesized that members in the Arabidopsis ACBP family have nonoverlapping functions in the plant cell. (aocs.org)
We established a novel rosetting model by coculturing HLA-II-matched peripheral blood mononuclear cells with HL cell lines and showed IS formation with activation of rosetting T cells. (ashpublications.org)

While overexpression of MYC has previously been shown to drive medulloblastoma formation in mice, the functional significance of SMARCA4 mutations and their suitability as a therapeutic target remain largely unclear. (bvsalud.org)

Six trimeric SAXS models nearly filled the space below an average FF-TEM image of the rosette CSC. (osti.gov)
In vitro models aim to mimic these processes and recapitulate brain organogenesis. (ehu.eus)

Stratifying the data for the frequency of rosette formation showed that the above results were not significantly altered by this phenomenon. (ox.ac.uk)

With abscess formation, the sputum may become blood streaked. (pediagenosis.com)

We obtained more direct evidence through generating improved transmission electron microscopy (TEM) images and image averages of the rosette-type CSC, revealing the frequent triangularity and average cross-sectional area in the plasma membrane of its individual lobes. (osti.gov)

A six-lobed membrane spanning cellulose synthesis complex (CSC) containing multiple cellulose synthase (CESA) glycosyltransferases mediates cellulose microfibril formation. (osti.gov)

Anatomy14

Organisms4

Diseases3

Chemicals and Drugs23

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes7

Pluripotent3

Vivo6

Antigen3

Endothelial cells4

Neurulation3

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Neural4

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Protein3

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Neurons1

Human4

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