Vitro hek293 cells. Medical search. Frequent questions

A ) HEK293 cells were transfected with HA-Cdk5, then cotransfected with either myc-p35 or myc-cyclin I. Following an IP to the myc epitope tag on either p35 or cyclin I, in vitro kinase assays were performed using either histone H1 (HH1) or tau as substrates. (jci.org)
DMH-1 is a selective inhibitor of bone morphogenic protein (BMP) type-I receptor activin receptor-like kinase 2 (ALK2) receptor (IC 50 = 108 nM or 12.6 nM in in vitro kinase assays). (tocris.com)
Strengths: Many synthetic variants were generated to accomplish the best antinociceptive peptides including its in vitro and in vivo assays. (elifesciences.org)
Designed for 24- and 96-well formats , our innovative solution revolutionizes cell culture transport and assays. (readycell.com)
HEK 293 cells were used for luciferase assays to study miR-216a-5p binding sites with H19 and ACTA2. (iasp-pain.org)
CONCLUSIONS: A recombinant HS/CS PG interacted strongly and functionally with BMP-2 in binding and cell-based assays, and, in vivo, the pln.247 expression plasmid significantly improved the dose-effectiveness of BMP-2 osteogenic activity for in vivo de novo bone generation when delivered together on a scaffold as a single-phase. (nova.edu)
Antigen secretion was stable for at least 10 cell passages, as measured by enzyme-linked immunosorbent assays and immunofluorescence assays. (cdc.gov)

OATP2B1 variants were expressed in human derived HEK293 cell line using baculovirus expression system. (helsinki.fi)

Because the neurobiological substrate of cariprazine has remained elusive, we took advantage of PharmacoSTORM to provide in vivo evidence that cariprazine predominantly binds to D 3 dopamine receptors on Islands of Calleja granule cell axons but avoids dopaminergic terminals. (nature.com)
Although the in vitro binding data were consistent with the in vivo study, it did not identify the binding characteristics of each of the bridged receptors. (aspetjournals.org)
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. (sigmaaldrich.com)
Encapsulating Polyethyleneimine-DNA Nanoplexes into PEGylated Biodegradable Microparticles Increases Transgene Expression In Vitro and Reduces Inflammatory Responses In Vivo. (escholarship.org)
The main limitation of our study was that experiments were conducted in vitro and further in-vivo studies are required in the future. (iasp-pain.org)
The binding capacity of ATG-101 to FcγRn prevents the degradation of IgG in vivo, while the deficiency of other FcγRs binding ensures that ATG-101 showed 4-1BB agonist activity only when crosslinked by PD-L1-positive cells," Hou added. (onclive.com)
We identified multiple, novel Thap1 species of apparent M r 32 kDa, 47 kDa, and 50-52 kDa in vitro and in vivo , and verified the previously identified species at 29-30 kDa in neurons. (biomedcentral.com)
ReNeuron Group plc (AIM: RENE), a UK based leader in stem cell derived exosome technologies, announces that Dr Samantha Thomas will be presenting in vivo data exemplifying the cellular and tissue targeting capabilities and highlighting the significant advantages of ReNeuron's CustomEX™ drug delivery platform, at Cell 2023 in London. (euroland.com)
Confirmation in vitro and in vivo that exosome targeting is dependent on cell source and selection of a specific exosome population results in the improved delivery of therapeutic payloads when compared to a conventional HEK293 exosome approach. (euroland.com)
Recent work has demonstrated that clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems1-3 can serve as the basis of a simple and highly efficient method for performing genome editing in bacteria, yeast and human cells, as well as in vivo in whole organisms such as fruit flies, zebrafish and mice4-13. (cdc.gov)
Os estudos in vitro estão livres da complexidade e fatores de confusão próprios dos modelos in vivo e ensaios clínicos. (bvsalud.org)

The in vitro method developed in this study remains in the research team as a tool to investigate the polymorphisms of the SLCO1B1-gene, the inhibition of the transporter and possible drug interactions. (helsinki.fi)
Cdk5 activity was reduced in glomeruli and brain lysates from cyclin I-deficient mice, and inhibition of Cdk5 increased in vitro the susceptibility to apoptosis in response to cellular damage. (jci.org)
Their in-vitro electrophysiological analysis is excellent, showing full selectivity profiles (including difficult to work with channels such as hNav1.8 and hNav1.9) from HEK293 cells and also showing inhibition of the TTX-S current with both mouse and human cultured DRG neurons. (elifesciences.org)
In transgenic HEK293 cells, it was demonstrated that the formation of ARNT homodimers is underlying PP2A inhibition. (uni-goettingen.de)
However, in the presence of Aza despite increased ERV-K113 env expression, an inhibition of HEK293 proliferation and a further restriction of invasion was found. (frontiersin.org)
Inhibition of both TrxR and Trx activity was observed in HeLa and HEK 293 cells treated with HgCl(2) or MeHg. (qxmd.com)
Human Grx1 showed similar reactivity as Trx1 with both mercurial compounds, with the loss of all free thiols and Grx dimerization in the presence of HgCl(2), but no inhibition of Grx activity was observed in lysates of HeLa cells exposed to mercury. (qxmd.com)

The relative molecular mass (M r ) of authentic exogenous Thap1 was previously established by in vitro translation of recombinant c-Myc-tagged human Thap1 protein from a human THAP1 cDNA. (biomedcentral.com)
RESULTS: Utilizing a purified recombinant human perlecan domain 1 (rhPln.D1) expressed from HEK 293 cells with HS and CS GAGs, tight binding and dose-enhancement of rhBMP-2 activity was demonstrated in vitro. (nova.edu)
The use of previously developed COS-1 cell lines secreting noninfectious recombinant viral antigen is limited due to diminished antigen secretion over time. (cdc.gov)

We previously showed that cyclin I does not regulate proliferation, but rather controls survival of podocytes, terminally differentiated epithelial cells that are essential for the structural and functional integrity of kidney glomeruli. (jci.org)
DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)
Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
Additionally, the findings showed an increase in the infiltration, proliferation, and activation of CD8+ T cells with ATG-101, as well as the infiltration of natural killer T cells and the CD8+/Treg ratio in tumor infiltrating lymphocytes. (onclive.com)

Human embryonic kidney Fibroblast cell line genetically modified with a truncated version of EBNA1 which grows in suspension and chemically defined serum-free medium. (polyplus-sartorius.com)

In this study the characteristics of the OATP1B1 transporter were reviewed and new in vitro research method to study protein functions was developed. (helsinki.fi)
The present study used in vitro expression systems to investigate interactions of members of the KDN series with δ-κ heterodimers through competition binding using selective ligands and the mitogen-activated protein kinase (MAPK) assay. (aspetjournals.org)
Similarly, cleaved caspase 3 protein levels and numbers of TUNEL-positive cells were increased in CIN mice, but suppressed in CIN mice injected with SF-MSCs compared with S-MSCs. (asn-online.org)
In vitro experiments showed that the increase in cleaved caspase 3 protein level induced by radiation of HEK293 cells was more strongly suppressed by conditioned medium from SF-MSCs compared with that from S-MSCs. (asn-online.org)
The viral spike (S) protein engages the human angiotensin-converting enzyme 2 (ACE2) receptor to invade host cells with ~10-15-fold higher affinity compared to SARS-CoV S-protein, making it highly infectious. (polyplus-sartorius.com)
Background: This study aimed to investigate the efficacy and safety of chemotherapy combined with programmed cell death protein 1 (PD-1) inhibitors in the treatment of advanced cervical cancer and the effect of optimal combination timing on prognosis. (bvsalud.org)
The specific PP2A inhibitor LB-100 induced the formation of ARNT homodimers in vitro and increased protein stability of Arnt. (uni-goettingen.de)
Human insulin, exogenously added to in vitro cultivated parasite larvae, (i) significantly stimulated parasite survival and growth, (ii) induced DNA de novo synthesis in Echinococcus, (iii) affected overall protein phosphorylation in the parasite, and (iv) specifically induced the phosphorylation of the parasite's Erk-like MAP kinase orthologue EmMPK1. (uni-wuerzburg.de)
Fluorescence microscopy studies of the actin protein revealed no changes of the immunolabelling profile in the renal cells cultured in the presence of Eu:HAp nanoparticles. (edu.pl)
BACKGROUND: Many growth factors, such as bone morphogenetic protein (BMP)-2, have been shown to interact with polymers of sulfated disacharrides known as heparan sulfate (HS) glycosaminoglycans (GAGs), which are found on matrix and cell-surface proteoglycans throughout the body. (nova.edu)
Here, we describe the generation of 39 flaviviral virus-like particle (VLP)- and nonstructural protein 1 (NS1)-secreting stable cell lines in HEK-293 cells representing 13 medically important flaviviruses. (cdc.gov)

Here, we use a human cell-based reporter assay to characterize off- target cleavage of Cas9-based RGENs. (cdc.gov)
This engineered human IgM constitutively expressed in a HEK-293 stable cell line can replace human-positive control sera in diagnostic serological techniques such as IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA). (cdc.gov)

Investigating the role of cell membrane proteins has increased over the last decade, as drugdrug interactions and genetic polymorphisms have been found to cause changes in drug pharmacokinetics and dynamics. (helsinki.fi)
Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)
Taken together, these data suggest that a cyclin I-Cdk5 complex forms a critical antiapoptotic factor in terminally differentiated cells that functions via MAPK signaling to modulate levels of the prosurvival proteins Bcl-2 and Bcl-XL. (jci.org)
Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. (medchemexpress.com)
Tricellular TJs (tTJs), specialized structural variants of TJs, are created by multiple transmembrane proteins (e.g., lipolysis-stimulated lipoprotein receptor [LSR] and tricellulin) within tricellular contacts in the mammalian epithelial cell sheet. (synanet2020.com)
In the mammalian epithelial cell sheet, TJs regulate molecular transport within the intercellular space and independent compartments of proteins and lipids localized to apical and basolateral membranes [4,5]. (synanet2020.com)
Finally, the in vitro biological effects of Eu:HAp nanoparticles were evaluated by focusing on the F-actin filament pattern and heat shock proteins (Hsp) expression in HEK293 human kidney cell line. (edu.pl)

ATG-101, given at 3.25 mg/kg or 13 mg/kg, was found to potentially inhibit tumor growth in h4-1BB transgenic mice bearing PD-1/PD-L1 inhibitor resistant B16F10 (melanoma) and EL4 (lymphoma) tumor cells. (onclive.com)

In this study we show that contactin coimmunoprecipitates with Na v 1.3 from postnatal day 0 rat brain where this channel is abundant, and from human embryonic kidney (HEK) 293 cells stably transfected with Na v 1.3 (HEK-Na v 1.3). (jneurosci.org)
The chimeric IgM produced from the stably expressing HEK293 cell line was evaluated for use as a surrogate human-positive control in a serologic diagnostic test. (cdc.gov)

Here, we introduce PharmacoSTORM super-resolution imaging that combines the complementary advantages of these approaches and enables cell-type- and compartment-specific nanoscale molecular measurements. (nature.com)
Importantly, molecular alterations often swing into opposite directions in a cell- and subcellular compartment-specific manner. (nature.com)
Address correspondence to C. Borner, Institute of Molecular Medicine and Cell Research, Center for Biochemistry and Molecular Cell Research, Albert-Ludwigs-University Freiburg, Stefan-Meier Strasse 17, D-79104 Freiburg, Germany. (rupress.org)
Abstract Tight junctions (TJs) are cellular junctions within the mammalian epithelial cell sheet that function as a physical barrier to molecular transport within the intercellular space. (synanet2020.com)

Human Embryonic Kidney cells (HEK) is a human derived mammalian cell-line that is widely used in the study of OATP1B1 transporter. (helsinki.fi)
Our findings indicated a novel mechanism by which Pyk2 regulates tTJ assembly and epithelial barrier function in the mammalian epithelial cell sheet. (synanet2020.com)
Intro The mammalian epithelial cell sheet consists of at least six types of cellular junctions: limited junctions (TJs), adherens junctions, desmosomes, hemidesmosomes, focal adhesions, and space junctions [1C3]. (synanet2020.com)
Dysregulation of any of these cellular junctions causes mammalian epithelial cell sheet dysfunction, which, in turn, causes various diseases [2]. (synanet2020.com)
In the present work, the effects of host insulin on Echinococcus metacestode vesicles and the proposed interaction between EmIR and mammalian insulin have been studied using biochemical and cell-biological approaches. (uni-wuerzburg.de)

Orexin 2 Receptor Agonist replaces Orexin-A to bind to hOX1R and hOX2R in a concentration-dependent manner in CHO cells overexpressing hOX1R and HEK-293 cells overexpressing hOX2R with K i values of 0.14 μM and 0.77 μM, respectively [1] . (medchemexpress.com)
Previous studies possess recognized that tyrphostin 9, the dual inhibitor Rabbit Polyclonal to ATG16L2 of Pyk2 (a nonreceptor tyrosine kinase) and receptor tyrosine kinase platelet-derived growth element receptor (PDGFR), suppresses LSR and tricellulin recruitment to tTJs in EpH4 (a mouse mammary epithelial cell collection) cells. (synanet2020.com)
Human insulin, exogenously added to in vitro cultivated The insulin receptor ortholog EmIR of the fox-tapeworm Echinococcus multilocularis displays significant structural homology to the human insulin receptor (HIR) and has been suggested to be involved in insulin sensing mechanisms of the parasite's metacestode larval stage. (uni-wuerzburg.de)
Furthermore, upon expression of EmIR/HIR receptor chimera containing the extracellular ligand binding domain of EmIR in HEK 293 cells, a specific autophosphorylation of the chimera could be induced through the addition of exogenous insulin. (uni-wuerzburg.de)
The importance of insulin signaling mechanisms for parasite survival and growth were underscored by in vitro cultivation experiments in which the addition of an inhibitor of insulin receptor tyrosine kinases led to vesicle degradation and death. (uni-wuerzburg.de)

Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973. (wikipedia.org)

Sequence verified plasmids were transfected into 293 cells using FectoPro. (polyplus-sartorius.com)
EpH4 cells were transfected with plasmids encoding LSR-GFP. (synanet2020.com)

Improved engraftment and therapeutic efficacy by human genome-edited hematopoietic stem cells with Busulfan-based myeloablation. (stanford.edu)
Proof-of-concept studies using human genome-edited hematopoietic stem cells have been hindered by the low efficiency of engraftment of the edited cells in the bone marrow and their modest efficacy in the CNS. (stanford.edu)
Functionally, overexpression of SH3p4 promotes agonist-induced internalization and modestly decreases the Gs coupling efficacy of beta1-ARs in HEK293 cells while having no effect on beta2-ARs. (duke.edu)

HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. (wikipedia.org)
HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
Graham performed the transfection a total of eight times, obtaining just one clone of cells that were cultured for several months. (wikipedia.org)
Transfection of HEK-Na v 1.3 cells with contactin increases the amplitude of the current threefold without changing the biophysical properties of the channel. (jneurosci.org)
In this study, we investigated the advantages of using polymeric microparticles carrying the luciferase reporter gene in increasing transfection efficiency in the readily transfectable HEK293 cell line and the difficult to transfect RAW264.7 cell line. (escholarship.org)
Transfection of heavy chain and light chain expression vectors was performed in HEK-293-6E cells using PEIpro. (polyplus-sartorius.com)

Through the generation of several unique and scalable exosome producer cell lines, our CustomEX™ platform can be optimised for specific tissues targets and payloads leading to improvements in therapeutic outcome and a reduction in off-target effects. (euroland.com)
Our work demonstrates that RGENs are highly active even with imperfectly matched RNA-DNA interfaces in human cells, a finding that might confound their use in research and therapeutic applications. (cdc.gov)
Understanding the scope of RGEN-mediated off-target effects in human and other eukaryotic cells will be critically essential if these nucleases are to be used widely for research and therapeutic applications. (cdc.gov)

J Cell Biol (2004) 164 (7): 1021-1032. (rupress.org)

The wild-type SLCO1B1-gene encoding the OATP1B1 was virulent with baculovirus into the cells by the Bac-to-Bac® Baculovirus Expression System. (helsinki.fi)
For expression in the Sf9 cells, the aim of the study was to clone the SLCO1B1-gene into the pFastBac vector. (helsinki.fi)
The expression of OATP1B1 transporter in HEK293 cells was successful. (helsinki.fi)
Contactin/F3, a cell adhesion molecule, has been shown to interact with and enhance surface expression of sodium channels Na v 1.2 and Na v 1.9. (jneurosci.org)
The expression of contactin and Na v 1.3 in the HEK-Na v 1.3 stable cell line significantly increases the current density. (jneurosci.org)
Compared with sub-lethal irradiation, Busulfan conditioning enhanced the engraftment of edited CD34+ cells in the bone marrow, as well the long-term homing and survival of bone-marrow-derived cells in viscera, and in the CNS, resulting in higher transgene expression and biochemical correction in these organs. (stanford.edu)
Primary cultured cells of endometrial tissues were analysed using RT-PCR and western blotting (WB) to determine expression of H19 and ACTA2. (iasp-pain.org)
Thirty-five cell lines (90%) had stable antigen expression over 10 passages, with three of these cell lines (7%) increasing in antigen expression and one cell line (3%) decreasing in antigen expression. (cdc.gov)

For many years it was assumed that HEK 293 cells were generated by transformation of either a fibroblastic, endothelial or epithelial cell, all of which are abundant in kidneys. (wikipedia.org)
The human umbilical vein endothelial cell and peripheral blood mononuclear cell coculturing system, which is the most sensitive system in evaluating the risk of drug-induced CRS, showed that ATG-101 did not induce non-specific inflammatory cytokine releases. (onclive.com)
RRM1 , a ubiquitously expressed subunit of ribonucleoside-diphosphate reductase, and CCAR1 (cell cycle and apoptosis regulator 1) have been identified as direct targets for regulation by Thap1 in endothelial or Jurkat cells. (biomedcentral.com)

We also investigated the ability of conditioned medium from S-MSCs or SF-MSCs to suppress apoptosis of X-ray-irradiated HEK293 cells. (asn-online.org)

Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. (wikipedia.org)

Further viability experiments showed an irreversible cell damage, since no recovery occurred after up to 71 h in YTX-free medium. (units.it)

The estrogen-regulated lncRNA H19/miR-216a-5p axis alters stromal cell invasion and migration via ACTA2 in endometriosis. (iasp-pain.org)
Alterations in the H19/miR-216a-5p/ACTA2 pathway may mediate the regulation of eutopic endometrial stromal cell (euESC) invasion and migration and may represent a potential mechanism underlying fibrous tissue formation or fibrosis in women with endometriosis. (iasp-pain.org)

After 72 h, the cell lysates were prepared and immunoprecipitated (IP) with anti-GFP or normal rabbit IgG (IgG) antibody, followed by immunoblotting analysis using anti-GFP or Pyk2 antibody. (synanet2020.com)

Furthermore, the aim was to study the effects of six naturally occurring nonsynonymous single nucleotide variants on OATP2B1 transport function in vitro. (helsinki.fi)

3D6 was the best binder to plaques and in vitro, whilst mC2 bound the least strongly. (polyplus-sartorius.com)

293T (or HEK 293T) is a derivative human cell line that expresses a mutant version of the SV40 large T antigen. (wikipedia.org)
After presumably adapting to tissue culture, cells from this clone developed into the relatively stable HEK 293 line. (wikipedia.org)
However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. (wikipedia.org)
An embryonic adrenal precursor cell therefore seems the most likely origin cell of the HEK 293 line. (wikipedia.org)
The first reference to the cell line as "293T" may be its use to create the BOSC23 packaging cell line for producing retroviral particles. (wikipedia.org)
The Sf9 cell line is isolated from Spodoptera frugiperda insect and is one of the standard in vitro tools in a genetic engineering study. (helsinki.fi)
The goal was accomplished via overexpression and knock-down of Thap1 in the HEK293T cell line and in mouse striatal primary cultures and western blotting of embryonic Thap1 -null tissue. (biomedcentral.com)
Antigen secretion in the HEK-293 cell lines was higher than in previously developed COS-1 cell line counterparts. (cdc.gov)
Antigen production was more stable and statistically higher in these newly developed cell lines than in their COS-1 cell line counterparts. (cdc.gov)

Also, our R&D team continuously develops new plates with different cell lines and improves the formulation to extend shipping time while preserving cell viability and functionality. (readycell.com)
Transfected cells were cultured until the viability had dropped to around 50 % and culture media harvested by centrifugation and ultrafiltration. (polyplus-sartorius.com)

Dr. Pasqualina Colella is a scientist with extensive expertise in cell therapy and gene therapy approaches for the treatment of inherited diseases. (stanford.edu)
She is currently Research Scientist at Stanford University, where she is developing innovative cell therapy approaches to address neuropathic lysosomal storage diseases (LSDs) using hematopoietic stem cell transplantation (HSCT) and genome editing. (stanford.edu)

Diverse cell cultures derived from wildlife, livestock, and humans were susceptible to NPV, with pronounced permissiveness in swine and rodent cells. (cdc.gov)

HS GAGs, and some more highly sulfated forms of chondroitin sulfate (CS), regulate cell function by serving as co-factors, or co-receptors, in GF interactions with their receptors, and HS or CS GAGs have been shown to be necessary for inducing signaling and GF activity, even in the osteogenic lineage. (nova.edu)

2003). Notwithstanding many in vitro studies highlighted numerous intracellular targets, YTX mechanism of action is unclear and the effects on the cardiac functional properties remain unknown. (units.it)
These results show a very cell-specific response to YTX if compared to previous studies, and a severe damage to in vitro cardiomyocytes. (units.it)

This study was performed on neonatal rat cardiomyocytes to study toxin effects on various fundamental aspects of cardiac muscle cells activity: cell beating, intracellular Ca2+ and cAMP levels, cell vitality, mitochondrial membrane potential and type of cell death occurrence. (units.it)

ReNeuron has developed a proprietary stem cell-derived, exosome-based, drug delivery platform with customisable cellular targeting capabilities for the delivery of complex drug modalities. (euroland.com)

Porosity did not influence cell counts in bronchoalveolar lavage fluid in the absence of PEG, but in particles containing PEG, non-porous particles recruited fewer inflammatory cells than their porous counterparts. (escholarship.org)

The use of these cell lines for production of flaviviral antigens will expand serological diagnostic testing of flaviviruses worldwide. (cdc.gov)

Permissiveness for NPV replication in sand fly cells, but not mosquito cells, suggests a vector-specific adaptation. (cdc.gov)
This suggests ATG-101 has the potential ability to reverse T-cell dysfunction and exhaustion. (onclive.com)

Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue. (wikipedia.org)
In the experimental part of this thesis the goal was to express OATP1B1 transporter in Sf9 and HEK293 cell lines. (helsinki.fi)
Antibodies and cell lines. (jneurosci.org)
However, our study showed that primary cultured cells represented endometriosis cells more clearly than cell lines. (iasp-pain.org)
Transfecting these cell lines with a codon optimized ERV-K113 env overexpressing CMV vector was performed with or without 5'-Aza-2'-deoxycytidine (Aza) treatment to sustain promoter de-methylation. (frontiersin.org)

GR was inhibited by HgCl(2) and MeHg in vitro, but no decrease in GR activity was detected in cell extracts treated with mercurials. (qxmd.com)

Because of the expected impact on energy and environment through the development of solar cell components and photocatalysts, intense efforts have been devoted to the preparation of hybrid chalcogenide heterostructures containing two or more chalcogenide nanostructured domains. (acs.org)

The HEK 293 pattern most closely resembled that of adrenal cells, which have many neuronal properties. (wikipedia.org)
Brain circuits consist of more than hundred distinct neuronal and glial cell types 1 , which communicate via billions of synapses built up from highly compartmentalized nanoscale signaling platforms 2 . (nature.com)

Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. (frontiersin.org)
Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. (frontiersin.org)

DMH-1 is also offered as part of the Tocriscreen 2.0 Max , Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library . (tocris.com)
The cells were then immunostained with anti-LSR (C, LSR) and anti-tricellulin (D, TRI) antibodies, and observed p32 Inhibitor M36 using confocal microscopy. (synanet2020.com)
Pyk2 inactivation by its specific inhibitor or repression by RNAi inhibited the localization of LSR and downstream tricellulin to tTJs without changing their manifestation level in EpH4 cells. (synanet2020.com)

These findings show that PharmacoSTORM helps to quantify drug-target interaction sites at the nanoscale level in a cell-type- and subcellular context-dependent manner and within complex tissue preparations. (nature.com)

Human CX3CL1 / NTN derived in Human Cells. (creativebiomart.net)
We readily detected off-target alterations induced by four out of six RGENs targeted to endogenous loci in human cells by examination of partially mismatched sites. (cdc.gov)
Although Maraffini and colleagues7 recently performed a systematic investigation of Cas9 RGEN specificity in bacteria, the specificities of RGENs in human cells have not been extensively defined and, to our knowledge, bona fide off-target mutations induced by Cas9 have not been identified in any eukaryotic cell or organism. (cdc.gov)
To begin to define the specificity determinants of RGENs in human cells, we sought to perform a large-scale test in which we assessed the effects of systematically mismatching various positions within multiple gRNA/target DNA interfaces. (cdc.gov)

Cyclin I is an atypical cyclin because it is most abundant in postmitotic cells. (jci.org)

Our protocol ensures reliable results, maintaining cell integrity from transportation to experimentation. (readycell.com)
Additional results showed that ATG-101 mediates effective and PD-L1-dependent activation of CD8+ T cells. (onclive.com)
These results were observed by inducing exhausted T cells by human CD3+ T cells culturing with anti-CD3/CD28 beads for 6 days. (onclive.com)

Through its conditionally immortalised induced pluripotent stem cell (iPSC) platform, the Group can make allogeneic tissue cells of choice and has the potential to produce exosomes with tissue specific targeting ability. (euroland.com)

cells were incubated with DMSO (Control) or 1C80 M GSK2256098 (GSK) for 120 min. (synanet2020.com)
A) EpH4-Cl3 cells were cultured for 24 h and after incubated with DMSO (Control) or 20 M PF-43. (synanet2020.com)

Very lipophilic drugs enter the cell membrane through passive diffusion, but polar or ionized organic compounds can enter the cell membrane only by transporters. (helsinki.fi)
Also blocks cell membrane-located capsaicin-activated cation channels (IC 50 = 14 nM) and voltage-sensitive Ca 2+ channels to inhibit neurotransmitter release. (tocris.com)

Additionally, ATG-101 induced IL-2 and INF-γ secretion by exhausted T cells in the presence of PD-L1-positive cells. (onclive.com)

Homeopath Robert Medhurst shares summaries of some recent homeopathy research done with people, and in vitro, in plants and with animals. (hpathy.com)
There is a large body of human, animal, plant and in-vitro trials that attest to the effectiveness of homeopathy and homeopathically potentised substances. (hpathy.com)
2007 utilizando as palavras-chave "homeopathy" AND selecionados, contato direto com autores, etc. "in vitro" em qualquer língua. (bvsalud.org)

the original HEK 293 cell clone was from his 293rd experiment. (wikipedia.org)

HEK293 cells expressing OATP1B1 transporter are well suited for the study of the SLCO1B1-gene. (helsinki.fi)

Anatomy8

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Chemicals and Drugs39

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Phenomena and Processes26

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Proliferation5

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Assay2

Proteins7

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Stably2

Molecular4

Mammalian5

Receptor5

Embryonic kidney 293 cells1

Plasmids2

Efficacy3

Transfection6

Therapeutic3

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Expression8

Endothelial3

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Invasion2

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Strongly1

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Humans1

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