Embryonic FibroblastsUntreated fibroblast cellsAssayPresence of fibroblastsPhenotypeTissueCollagenSkin fibroblastsPatient fibroblastsStromal fibroblastsGingival fibroblastsCellsCell proliferationLiving cellCellularProliferationHumanAssaysStudyCytotoxicityTissuesGeneticIncubationMiceVivo studiesGeneMurineSusceptibilityStimulatesStudiesMethodsLungInflammationMetastasisCardiacWound closureBoneInvestigationLaboratoryGrowthActivationAbsence
Embryonic Fibroblasts3
- The present study aims to compare the proliferative effects of chitosan in two forms, membranous and solution forms, on Swiss 3T3 mouse embryonic fibroblasts. (balkanmedicaljournal.org)
- The membranous form of chitosan induced a significant proliferative effect and increased the ratio of cell-to-cell junctions of Swiss 3T3 mouse embryonic fibroblasts. (balkanmedicaljournal.org)
- After ingestion of melanoma-derived exosomes during incubation, mouse embryonic fibroblasts cells emerged cellular invasiveness with the presentation of a marked increase in miR-21 expression. (dovepress.com)
Untreated fibroblast cells1
- The invasion analysis showed that exosome-treated fibroblast cells had greater invasive capability as compared to untreated fibroblast cells, with the higher expressions of MMP2 and MMP9. (dovepress.com)
Assay4
- In vitro Scratch assay determined the effect of plant extracts on migration of cells and its underlying mechanism was determined by RT-PCR analysis. (phcogj.com)
- In Vitro Toxicity Studies: Cell viability (dye exclusion): trypan blue assay etc. (cdc.gov)
- In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc. (cdc.gov)
- Cell viability of L-929 fibroblast cells was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the difference between the groups was tested by analysis of variance and Tukey tests (a = 0.05). (ommegaonline.org)
Presence of fibroblasts2
- The ability of the organism to survive in the presence of fibroblasts was not related to its infectivity. (nih.gov)
- The dermal layer allows for host incorporation of the material because of the presence of fibroblasts in the extracellular matrix and vasculature. (medscape.com)
Phenotype4
- CONCLUSIONS: The findings from this study provide proof-of-concept that miRNAs have the capability of directly converting fibroblasts to a cardiomyocyte-like phenotype in vitro. (duke.edu)
- We hypothesize that tumor-derived exosomes endow the fibroblasts in tumor microenvironment with invasive phenotype to the benefit of tumor metastasis. (dovepress.com)
- Our results suggest that tumor-derived exosomes may facilitate stromal fibroblasts an aggressive phenotype to equip the tumor progression. (dovepress.com)
- Regulation of gingival fibroblast phenotype by periodontal ligament cells in vitro. (bvsalud.org)
Tissue7
- Hypoxia treatment of normal peritoneal fibroblasts results in alterations that are characteristic for fibroblasts obtained from postoperative peritoneal adhesion tissue. (elsevierpure.com)
- In this study, we have shown that hypoxia treatment enhances normal peritoneal fibroblast elimination by lymphokine-activated killer cells to the level of adhesion tissue fibroblasts, suggesting a pivotal role of hypoxia in the adhesion development. (elsevierpure.com)
- In 2009, co-author Gautier claimed that the discovery was based on fibroblast tissue samples that she had prepared and noticed the discrepancy in chromosome count on. (wikipedia.org)
- These in vitro data on BMSC and GF could explain the promotive effect of EMD on bone formation and connective tissue regeneration, respectively. (tau.ac.il)
- MiR-21 promoted invasion of fibroblasts by down-regulation of tissue inhibitor of metalloproteinase 3 (TIMP3) expression and increasing of matrix metalloprotein (MMP) expression in fibroblast cells via melanoma-derived exosomes in a time-dependent manner. (dovepress.com)
- Many of these genes, including those for activin A (Inhba), tumor necrosis factor-stimulated gene 6 (Tnfaip6), matrix metalloproteinase 19 (Mmp19), tissue inhibitor of metalloproteinases 1 (Timp1), and podoplanin (Pdpn), were significantly FGF-2 dependent following injury to cartilage in vitro and to joint tissues in vivo. (ox.ac.uk)
- Evaluation of the efficacy of proposed agents in an in vitro model is convenient and allows assessment of the mechanical properties of the bone-tendon interface as well as the effect on fibroblast proliferation and collagen content of the tissue. (researchgate.net)
Collagen4
- Therefore, we cultured fibroblast cells and added the particles in order to investigate the effect of cell proliferation ability and production of collagen fibers. (jarde.jp)
- In this study, it was observed that cultured fibroblasts were enhanced cell proliferation and collagen fiber production by adding aragonite particles. (jarde.jp)
- Objectives: To investigate the potential role of a commercially available preparation of micronized Amniotic Membrane Allograft (AMA) (Amniofix, MiMedx, USA) in the biological augmentation of an in vitro model of rotator cuff repair with respect to cellular pro- liferation, collagen content and mechanical properties of the bone- tendon interface. (researchgate.net)
- The collagen content of the BTC and cellular proliferation of fibroblasts were assessed using colorimetric assays. (researchgate.net)
Skin fibroblasts3
- Since B. burgdorferi first infects skin, the possible protective effect of skin fibroblasts from an antibiotic commonly used to treat Lyme disease, ceftriaxone, was examined. (nih.gov)
- Cell cycle analysis of in vitro cultured goral (Naemorhedus caudatus) adult skin fibroblasts. (druglib.com)
- The present study was undertaken to examine cell cycle characteristics of endangered Goral (CITES Appendix I) adult skin fibroblasts. (druglib.com)
Patient fibroblasts1
- Second, we used directly converted neurons from sporadic patient fibroblasts to study of age-related disease relevant pathology. (lu.se)
Stromal fibroblasts2
- Seminal plasma promotes decidualization of endometrial stromal fibroblasts in vitro from women with and without inflammatory disorders in a manner dependent on interleukin-11 signaling. (il-11.com)
- Is the seminal plasma (SP) and its constituents affect decidualizing capacity and transcriptome of Human main endometrial stromal fibroblasts (eSFs)? (il-11.com)
Gingival fibroblasts4
- Due to the fact that many different results are described for different concentrations of alcohol at different times, an attemptwas made to visualise the direct impact of 7.2% and 22% alcohol on human gingival fibroblasts. (aaem.pl)
- PANsystem 2000 was used for visualisation of the reaction of human gingival fibroblasts isolated from gingiva on ethanol in 2 different concentrations. (aaem.pl)
- The obtained results suggest that daily usage of 7.2% alcohol contained in mouthwashes is non-toxic for gingival fibroblasts, and could be recommended after periodontal surgery. (aaem.pl)
- In this study, we investigated the in vitro effects of EMD on rat bone marrow stromal cells (BMSC) and gingival fibroblasts (GF). (tau.ac.il)
Cells18
- To estimate the effect of the asthmatic microenvironment on the invasiveness of lung cancer cells, the present study compared the behavior of human non‑small cell lung cancer A549 cells exposed to the signals from human bronchial fibroblasts (HBFs) derived from non‑asthmatic donors (NA HBFs) and from asthmatic patients (AS HBFs). (spandidos-publications.com)
- These data indicated that the interactions between lung cancer cells and HBFs in asthmatic bronchi may facilitate the colonization of lung tumors by fibroblasts. (spandidos-publications.com)
- However, the contribution of asthmatic bronchial fibroblasts in systemic dissemination of lung cancer cells remains unaddressed. (spandidos-publications.com)
- METHODS AND RESULTS: Using a combinatorial strategy, we identified a combination of miRNAs 1, 133, 208, and 499 capable of inducing direct cellular reprogramming of fibroblasts to cardiomyocyte-like cells in vitro. (duke.edu)
- Genetic tracing analysis using Fsp1Cre-traced fibroblasts from both cardiac and noncardiac cell sources strongly suggests that induced cells are most likely of fibroblastic origin. (duke.edu)
- Currently, "in vitro" studies are performed on RA cells cultured in the presence of either recombinant proinflammatory cytokines-conditioned medium or medium alone. (unimi.it)
- These results emphasize the importance of using RA synovial fluid in "in vitro" studies involving RA cells, in order to reproduce faithfully the physiopathological environmental characteristic of RA joints. (unimi.it)
- Cytotoxicity assays were performed by using cell culture medium containing L-929 line cells (mouse fibroblast). (bvsalud.org)
- Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
- Mutagenicity was observed in the fibroblast cells, but the greater toxicity of PhIP to the epithelial cells prevented a definitive evaluation of mutagenicity. (uoguelph.ca)
- RT-PCR analysis showed that cytochrome P450 enzymes 1A1 and 1B1 are expressed at low levels in both cell lines, with greater 1B1 expression seen in the fibroblast cells. (uoguelph.ca)
- Bistite II DC, and Multi Bond II, after polymerization on cultured L-929 fibroblast cells. (ommegaonline.org)
- The biological safety of dentin-bonding agents has been extensively studied [3,4] , but reports on the biological safety of new resin-based cements to cultured L-929 fibroblast cells are still rare. (ommegaonline.org)
- To evaluate the cytotoxicity of resin-based cements on cultured L-929 fibroblast cells completely, this study evaluates the cytotoxicity of two resin-based cements (Bistite II DC, and Multi Bond II) after polymerization on cultured L-929 fibroblast cells. (ommegaonline.org)
- In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. (brill.com)
- Basic fibroblast growth factor (Fgf2) is required for the generation of founder cells within the dorsal pseudostratified ventricular epithelium, which will generate the cerebral cortex, but the ganglionic eminences are not affected. (jneurosci.org)
- Suitability of cells: Chinese hamster lung fibroblasts (CHL cells, clone No. 11) were used in this test because they are widely employed, for in vitro chromosomal aberration tests, show quite high sensitivity to chemical mutagens, and a large amount of data is available about their chromosomal aberrations. (europa.eu)
- The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. (bvsalud.org)
Cell proliferation2
- Materials and Methods: Cell proliferation, migration and wound closure promoting effect of the preparations (0712-2, 1101- 4) and their succussed solvents (0712-1, 1101-3) were investigated on mouse NIH 3T3 fibroblasts. (highdilution.org)
- Conclusions: Our results demonstrate that the Similasan® Arnica plus low dilution homeopathic remedy exerted wound healing potential, which is a result of increased ability of fibroblasts to migrate without affecting cell proliferation. (highdilution.org)
Living cell1
- Spirochetes were not protected from ceftriaxone by glutaraldehyde-fixed fibroblasts or fibroblast lysate, suggesting that a living cell was required. (nih.gov)
Cellular4
- In vitro studies have demonstrated its effect on cellular growth and its stimulatory action on cellular layer formation. (balkanmedicaljournal.org)
- Fibroblasts are associated with the metastasis of cancer depending on their cellular plasticity. (dovepress.com)
- With further technological developments, these human in vitro cellular models shall be highly useful in the screening of compounds for personalized medicine, allowing optimum therapy with minimum or no adverse effects, and in the study of adverse outcomes in different strata of population. (brill.com)
- Results: No significant effect on cellular proliferation was noted with the addition of AMA to fibroblasts in culture (p = 0.7). (researchgate.net)
Proliferation1
- In the present study, we examined the effect of Aristolochia bracteolata extract on Human dermal fibroblast (HDF) and Human keratinocyte cell line (HaCaT) proliferation and migration during in vitro wound healing and its underlying mechanism. (phcogj.com)
Human14
- Human foreskin fibroblasts protected B. burgdorferi from the lethal action of a 2-day exposure to ceftriaxone at 1 microgram/mL, 10-20 x MBC. (nih.gov)
- The modification produced by laser in human fibroblasts in culture have also been investigated. (ox.ac.uk)
- The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
- In murine and human lung fibroblasts, DE induced the release of AREG and inflammatory cytokines. (cdc.gov)
- Fibroblast recellularization of primary human lung mesenchymal matrix scaffolds and wound closure was inhibited by DE and enhanced with recombinant AREG alone. (cdc.gov)
- With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
- 2) Methods: A literature search was conducted and included any study that evaluated the effects or mechanisms of antioxidants in the healing process (in vitro, animal models or human studies). (mdpi.com)
- Non-confluent normal human dermal fibroblasts (NHDF) were treated for 24 hr with IL-1 (0.1 ng/mL), as an inducer of IL-8 production, in the presence or absence of palmitoyl tripeptide-8 (10 -7 M) or α-MSH (10 -11 M), as the positive control. (cosmeticsandtoiletries.com)
- In vitro Studies of Protein Transfer Across Human Fetal Membranes. (researchgate.net)
- By viral expression of neural fate determinants, it is possible to directly reprogram mouse and human fibroblasts into functional neurons, also known as induced neurons. (lu.se)
- In this study, we show that transplanted human fibroblasts and human astrocytes, which are engineered to express inducible forms of neural reprogramming genes, convert into neurons when reprogramming genes are activated after transplantation. (lu.se)
- In this thesis, human glia-to-neuron direct conversion and engineered viral vectors are explored using pre-clinical in vitro and ex vivo models. (lu.se)
- Human fibroblasts (0-20g/ml) were used to assess the predictive value of in vitro to in vivo modeling systems. (cdc.gov)
- Human fibroblast monocultures were found to be insufficient to fully replicate in vivo fibrosis outcomes suggesting in vitro predictive potential depends upon more advanced cell culture in vitro models. (cdc.gov)
Assays2
Study8
- PANsys 3000 is a multi-system fully-automated cell culture device used for in vitro culture and to study a variety of cell lines under conditions similar to in vivo . (aaem.pl)
- OBJECTIVE: The aim of this study was to develop a strategy that identified and evaluated the potential of specific micro (mi)RNAs capable of inducing reprogramming of cardiac fibroblasts directly to cardiomyocytes in vitro and in vivo. (duke.edu)
- In this study, we evaluated the use of synovial fluid, derived from RA patients, as optimal culture condition to perform "in vitro" studies on RA synovial fibroblasts. (unimi.it)
- It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. (nature.com)
- In vitro study. (balkanmedicaljournal.org)
- This study is limited to in vitro analysis. (il-11.com)
- Under the conditions of the study the test material induced chromosomal aberration in Chinese hamster lung fibroblasts. (europa.eu)
- The use of AMA in this in vitro study has not shown any benefit that would support its potential in improving the biology of cuff repair. (researchgate.net)
Cytotoxicity1
- For the direct-acting mutagen 'N'-ethyl-' N'-nitrosourea, all cell lines were equally sensitive to mutagenesis in the 'cII' gene but the fibroblast cell line showed greater resistance to cytotoxicity. (uoguelph.ca)
Tissues1
- Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. (bvsalud.org)
Incubation1
- It concentrates in phagocytes and fibroblasts, as demonstrated by in vitro incubation techniques. (medscape.com)
Mice1
- CONCLUSION: FGF-2-dependent gene expression occurs in vitro and in vivo in response to cartilage/joint injury in mice. (ox.ac.uk)
Vivo studies1
- In this review, we discuss the various factors of BNNT production that can influence its toxicity followed by summarizing the toxicity findings from in vitro and in vivo studies conducted to date, including a review of particle clearance observed with various exposure routes. (cdc.gov)
Gene1
- Fibroblast growth factor 2 drives changes in gene expression following injury to murine cartilage in vitro and in vivo. (ox.ac.uk)
Murine1
Susceptibility1
- To investigate the basis of this differential susceptibility, three cell lines were developed from the oral epithelium (BBR/OE), mammary epithelium (BBR/ME), and stromal mammary fibroblasts (BBR/MFib) of transgenc Big Blue rats. (uoguelph.ca)
Stimulates1
- Under our clinical and experimental conditions, laser stimulates the secretion of fibroblasts, both in vivo and in vitro, for reasons that are still the object of research. (ox.ac.uk)
Studies4
- In vitro scratch wound healing studies showed wound closure of 50.38%±1.39 and 69.81%±1.89 at a concentration of 25 μg/mL after 24 h and 48 h, respectively. (phcogj.com)
- Clinical studies have shown that SP may promote implantation, and in vitro studies have shown that SP can promote decidualizing, steroid hormone driven program differentiation of the ESF which is essential for embryo implantation and impaired in women with disorders of PCOS inflammatory and endometriosis. (il-11.com)
- In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc. (cdc.gov)
- Collectively, these studies demonstrate that inhibition of AREG reduced, whereas AREG supplementation promoted, the airway inflammatory recovery response following environmental bioaerosol exposure, and AREG enhanced fibroblast function, suggesting that AREG could be targeted in agricultur al workers repetitively exposed to organic dust environments to potentially prevent and/ or reduce disease. (cdc.gov)
Methods2
- In vitro methods are reproducible, cost-effective, relevant, and suitable for the evaluation of basic biological properties of dental materials. (ommegaonline.org)
- Methods: The in vitro model consisted of artificial bone-tendon constructs (BTC). (researchgate.net)
Lung1
- Accordingly, the activation of fibroblasts/myofibroblasts, which is characteristic for the asthmatic process, may facilitate lung cancer progression. (spandidos-publications.com)
Inflammation2
- During the asthmatic process, epithelial damage is followed by the thickening of asthmatic bronchial walls, which is mechanistically linked to the local inflammation and the activation of bronchial fibroblasts ( 2 - 4 ). (spandidos-publications.com)
- Local inflammation induces the differentiation of bronchial fibroblasts into α-smooth muscle actin (α-SMA) positive myofibroblasts and increases their longevity ( 5 ) through paracrine (for example TGFβ-dependent) and cell adhesion-dependent mechanisms ( 3 , 6 , 7 ). (spandidos-publications.com)
Metastasis1
- In particular, cancer-associated fibroblasts (CAFs) participate in cancer progression and metastasis ( 10 , 11 ). (spandidos-publications.com)
Cardiac2
- Scholars@Duke publication: MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fibroblasts to cardiomyocytes. (duke.edu)
- Importantly, administration of miRNAs into ischemic mouse myocardium resulted in evidence of direct conversion of cardiac fibroblasts to cardiomyocytes in situ. (duke.edu)
Wound closure1
- Used in vitro wound closure test was sensitive enough for low dilutions preparation, however for medium diluted preparation despite of a trend, no significant differences could be detected. (highdilution.org)
Bone1
- bone substitute) and a chick tendon fibroblast-seeded fibrin gel (tendon substitute). (researchgate.net)
Investigation1
- An investigation of the interaction between alcohol and fibroblasts in wound healing. (aaem.pl)
Laboratory1
- With very limited resources Gautier set up the first in vitro cell culture laboratory in France. (wikipedia.org)
Growth1
- One important injury-activated pathway involves the release of pericellular fibroblast growth factor 2 (FGF-2) from the articular cartilage. (ox.ac.uk)
Activation1
Absence1
- In the absence of fibroblasts, organisms did not survive. (nih.gov)