• Compared to the 3D binding model where the reactants are described in terms of concentration, the 2D model predicts less sensitivity of trimer formation to varying cell densities, which better matches changes in EC 50 from in vitro cytotoxicity assay data with varying E:T ratios. (springer.com)
  • In addition, when translating in vitro cytotoxicity data to predict in vivo active clinical dose for blinatumomab, the choice of model leads to a notable difference in dose prediction. (springer.com)
  • All fluoroquinolones showed moderate cytotoxicity after 24 h and more severe, significant toxicity after 72 h on tendon cells. (aspetjournals.org)
  • First steps in this direction are the characterization of relevant immune cells, optimization of culture conditions and in vitro differentiation from precursor to mature immune cells. (uni-potsdam.de)
  • Precursor cells and their phenotypes during the differentiation of hematopoietic stem cells towards immature B cells. (uni-potsdam.de)
  • They play an important role in many different vital cell processes including migration, proliferation, differentiation, and apoptosis. (harvard.edu)
  • Local inflammation induces the differentiation of bronchial fibroblasts into α-smooth muscle actin (α-SMA) positive myofibroblasts and increases their longevity ( 5 ) through paracrine (for example TGFβ-dependent) and cell adhesion-dependent mechanisms ( 3 , 6 , 7 ). (spandidos-publications.com)
  • In summary, co-culture with TPSCs induced molecular changes associated with EMT in pancreatic cancer cells, regardless of differentiation status, and the organotypic model demonstrated the influence of microenvironmental factors, such as glucose, stellate cells and macrophages, on pancreatic cancer cell invasion. (lu.se)
  • The murine embryonic stem (ES) cell line, ES-D3 was used in this project as a model for (a) investigating controversial aspects of current protocols used for the derivation of (3 cell types from ES cells and (b) investigating the potential to regulate the differentiation of ES cells into endodermal derivatives 1 e 0-1 ike cells, using extra-cellular factors previously associated with various aspects of pancreatic development. (dcu.ie)
  • Treatment of these aggregates with retinoic acid (RA) was previously shown to induce endodermal differentiation We exposed RA_EBs to secondary treatment with agents associated with pancreatic endocrine differentiation, e g Activin A (AA), Sodium Butyrate (SB) and Betacellulin (BTC), with the aim of developing a novel protocol for the regulated derivation of insulin-expressing cell types. (dcu.ie)
  • Applying 2-stage regulated differentiation protocols developedduring the course of the study, we demonstrated the derivation of an intermediate multi-potential population (RAJEBs) from undifferentiated ES cells that preferentially gave rise to pancreatic endocrine insulin-expressing cell types in the presence of SB and neuronal-like cell types in the presence of AA or BTC. (dcu.ie)
  • This loss in the glucose stimulated insulin secretion (GSIS) phenotype correlates with changes in morphology, increased proliferation and increased alkaline phosphatase (AP) activity, suggesting that the loss of GSIS may be due to de-differentiation of the high passage MIN6 cells. (dcu.ie)
  • AMSBIO also offer a complete suite of cell reprogramming and neural differentiation services to meet users neural research needs. (technologynetworks.com)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Furthermore, treatment of s.c xenografts derived from A549 cells with intratumor injections of terpinen-4-ol significantly inhibited tumor growth compared with the control group. (hindawi.com)
  • Stimulation of naive CD4 cells by specific antigen results in proliferation and changes in cell surface marker expression as the cells differentiate into effector and memory cells. (unboundmedicine.com)
  • AU - Lee,W T, AU - Pelletier,W J, PY - 1998/9/23/pubmed PY - 1998/9/23/medline PY - 1998/9/23/entrez SP - 1 EP - 11 JF - Cellular immunology JO - Cell Immunol VL - 188 IS - 1 N2 - Stimulation of naive CD4 cells by specific antigen results in proliferation and changes in cell surface marker expression as the cells differentiate into effector and memory cells. (unboundmedicine.com)
  • In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (nih.gov)
  • Macrophages and giant cells composed of macrophages are abundant in many neoplasms, but it is unknown whether they can phagocytose target cancer cells that adhere to one another or whether phagocytosis can outcompete proliferation. (aiche.org)
  • The role of the mTOR pathway in estrogen-driven proliferation and effects of combining RAD001 and letrozole were examined in vitro in two breast cancer models. (aacrjournals.org)
  • Effects of RAD001 and letrozole (alone and in combination) on the proliferation and survival of MCF7/Aro and T47D/Aro cells were evaluated using proliferation assays, flow cytometry, immunoblotting, and apoptosis analyses. (aacrjournals.org)
  • Metformin has been shown to have antiproliferative properties in several cancer cell lines, possibly related to its ability to inhibit cell proliferation pathways. (endocrine-abstracts.org)
  • Accordingly, we aimed to evaluate the effects of metformin in the survival and proliferation of PHEO cells. (endocrine-abstracts.org)
  • The effects of metformin on cell proliferation markers were analyzed by western blot. (endocrine-abstracts.org)
  • Moreover, metformin treatment reduced the activation of proteins of the AMPK/PTEN/AKT/mTOR pathway, which suggests growth and cell proliferation impairment. (endocrine-abstracts.org)
  • Altogether our results corroborate the hypothesis of an inhibitory effect of metformin on PHEO cellular proliferation in vitro . (endocrine-abstracts.org)
  • Results The results showed that the growth curve for cell proliferation and the formation of mineral nodules was similar for both cultures analyzed. (bvsalud.org)
  • CD4(+) T cells from DO11.10 TCR transgenic mice were labeled with the fluorescent dye carboxyfluorescein diacetate succinimidyl ester (CFSE) and were stimulated with specific antigen (OVA323-339). (unboundmedicine.com)
  • Yoneyama T, Kim M-S, Piatkov K, Wang H, Zhu AZX (2022) Leveraging a physiologically-based quantitative translational modeling platform for designing B cell maturation antigen-targeting bispecific T cell engagers for treatment of multiple myeloma. (springer.com)
  • NK cells are a subpopulation of lymphocytes that express the CD56 + antigen on their cell surface. (articlebiz.com)
  • These microspheres containing rAg85B were assessed for their ability to deliver antigen to macrophages for subsequent processing and presentation to the specific CD4 T-hybridoma cells DB-1. (rti.org)
  • T cell proliferative responses to Chlamydia trachomatis antigen in vitro in patients with a history of gynaecological chlamydia infection. (bmj.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
  • Thus, our in vitro and in vivo models utilizing gene-edited cells, monoclonal antibodies for opsonization and checkpoint blockade, and cell therapy begin to define the parameters required for successful immunotherapy of solid tumors including (i) macrophage density, (ii) inhibitory checkpoint disruption, and (iii) IgG-opsonization. (aiche.org)
  • This study compares in vitro and in vivo models of lipid digestion from almond particles within a complex food matrix (muffins) investigating whether the cell-wall barrier regulates the bioaccessibility of nutrients within this matrix. (nuthealth.org)
  • Melero-Martin JM , Khan ZA , Picard A , In vivo vasculogenic potential of human blood-derived endothelial progenitor cells . (avma.org)
  • Edwards N , Langford-Smith AWW , Wilkinson FL , Endothelial progenitor cells: new targets for therapeutics for inflammatory conditions with high cardiovascular risk . (avma.org)
  • As a first step toward the goal of defining the effects of DP treatment on haematopoiesis of preterm infants, we designed this in vitro study to determine whether DP affects the clonal growth of erythroid or granulocyte-macrophage progenitor cells in preterm infants, and if the preterm infant's progenitors respond to DP in a different manner from those of adults. (bmj.com)
  • 5 In this study we started with a highly enriched population of CD34+ progenitor cells 6 obtained by incubating the mononuclear fraction of blood and bone marrow with biotinylated anti-CD34 antibodies and running the CD34 labelled cells over an avidin column (Cellpro, Inc., Bothell, Washington, USA). (bmj.com)
  • AMSBIO offers an extensive range of iPSC derived neural cells including normal and Alzheimer's disease neural progenitor cells, and cerebral cortical neurons. (technologynetworks.com)
  • After users receive the neural progenitor cells and/or functional neurons from us, AMSBIO will also provide ongoing support to facilitate adaptation of the neural cells in their experimental systems. (technologynetworks.com)
  • List: Human dermal fibroblasts → multilineage blood progenitors (Oct4 and cytokine treatment) Mouse dermal fibroblasts → polygonal hyaline chondrogenic cells (Klf4, c-Myc, Sox9) Mouse dermal fibroblasts → cardiomyocytes (Oct4, Sox2, Klf4, JI1 and Bmp4) Fibroblasts → neural stem/progenitor cells (Oct4, Sox2, c-Myc, Klf4) Transdifferentiation Induced stem cells Pournasr, B. (wikipedia.org)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. (medsci.org)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
  • Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)
  • Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
  • We show that macrophages can eliminate melanoma cells in vitro from rapidly proliferating clusters formed on non-adhesive materials. (aiche.org)
  • As the scientists increased the CQA concentration, cancer cells died as a result of apoptosis. (sciencedaily.com)
  • The ability to induce apoptosis, or cell death, is a promising tactic for therapeutic interventions in all types of cancer, she said. (sciencedaily.com)
  • Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
  • Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
  • Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
  • Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
  • In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
  • These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
  • Organic solvent-induced proximal tubular cell apoptosis via caspase-9 activation. (cdc.gov)
  • Acute solvent exposure induced activation of cytochrome P4502E1 causes proximal tubular cell necrosis by oxidative stress. (cdc.gov)
  • Solid tumors pose significant challenges for immunotherapies including those employing phagocytic macrophages as effector cells. (aiche.org)
  • Arvedson T, Bailis JM, Britten CD, Klinger M, Nagorsen D, Coxon A et al (2022) Targeting solid tumors with bispecific T cell engager immune therapy. (springer.com)
  • Eleven to 14 days after the final carcinogen treatment, the cells were removed from the collagen gels and injected into the cleared mammary fat pads of syngeneic hosts to assay for transformed cell populations. (eurekamag.com)
  • We present a simple and quantitative assay system that accurately models human endothelium by use of primary human umbilical vein endothelial cells (HUVECs) in cell culture plates coated with gelatin , a matrix that mimics basal lamina , the matrix that is tightly associated with the vascular endothelium and is critical for its proper function. (bvsalud.org)
  • Mainly analyzed by in vitro assays in tumor transplantation models, little is known about their function in autochthonous tumor models in vivo. (mdc-berlin.de)
  • Methods: Cell viability was evaluated by MTT and TRIPAN assays using the PC12-Adh PHEO cell line treated with metformin in increasing concentrations (0 30 mM). (endocrine-abstracts.org)
  • In a recent study, scientists showed that human colon cancer cells die when they are exposed to the approximate number of bioactive compounds present in one cup of mate tea, which has long been consumed in South America for its medicinal properties. (sciencedaily.com)
  • In the in vitro study, de Mejia and former graduate student Sirima Puangpraphant isolated, purified, and then treated human colon cancer cells with caffeoylquinic acid (CQA) derivatives from mate tea. (sciencedaily.com)
  • This in vitro study was published in Molecular Nutrition & Food Research , vol. 55, pp. 1509-1522, in 2011. (sciencedaily.com)
  • In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
  • Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
  • In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
  • Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)
  • In this study, we examined the acquisition of memory markers after the initial stimulation of naive T cells. (unboundmedicine.com)
  • The objectives of this study were to characterize how the αv integrins regulate each other in both a normal and pro-fibrotic environment in order or decipher any mutual regulation between the αv integrins and to assess if there is a good in vitro model to look at potential compensation mechanisms specifically in an inflammatory environment. (harvard.edu)
  • The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)
  • To estimate the effect of the asthmatic microenvironment on the invasiveness of lung cancer cells, the present study compared the behavior of human non‑small cell lung cancer A549 cells exposed to the signals from human bronchial fibroblasts (HBFs) derived from non‑asthmatic donors (NA HBFs) and from asthmatic patients (AS HBFs). (spandidos-publications.com)
  • The present study investigated the influence by the microenvironment on pancreatic cancer cell invasion in vitro. (lu.se)
  • Moreover, our study indicates that intrinsic toxicity to tendon cells varies across fluoroquinolones. (aspetjournals.org)
  • The purpose of this study was to investigate PDT using the photosensitiser, meta tetrahydroxyphenyl chlorin (mTHPC), on rat neurons and satellite glia, compared with human adenocarcinoma cell (MCF-7). (open.ac.uk)
  • The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. (tubitak.gov.tr)
  • This study investigated the inhalation toxicity of the emissions from 3-D printing with acrylonitrile butadiene styrene (ABS) filament using an air-liquid interface (ALI) in vitro model. (cdc.gov)
  • Mean absorbance values, which represent PDL cell viability for each tested medium at 5 °C in different periods of time. (scielo.br)
  • Determination of cell viability. (gc.ca)
  • Results and Discussion: Metformin at 20 mM induced an inhibition of 60% of cell viability after 48 h treatment, as compared to untreated controls, and increased cellular lipid peroxidation, while decreased O 2 consumption in PC12-Adh PHEO cells. (endocrine-abstracts.org)
  • Our results suggest that metformin has a moderate inhibitory effect on the viability of PC12-Adh PHEO cells. (endocrine-abstracts.org)
  • We used the pancreatic adenocarcinoma cell line PANC-1 as a model to apply different chemotherapeutic protocols (monotherapy and combined therapy) using 5-Fluorouracil, Oxaliplatin, Irinotecan, Gemcitabine and Abraxane. (isciii.es)
  • Specifically, we propose to model the second binding step with trans-avidity and as a two-dimensional (2D) process where the reactants are modeled as the cell-surface density. (springer.com)
  • The dose predicted by the 2D model aligns better with the approved clinical dose and the prediction is robust under variations in the in vitro to in vivo translation assumptions. (springer.com)
  • Jiang X, Chen X, Carpenter TJ, Wang J, Zhou R, Davis HM et al (2018) Development of a Target cell-Biologics-Effector cell (TBE) complex-based cell killing model to characterize target cell depletion by T cell redirecting bispecific agents. (springer.com)
  • Cytoadherence characteristics of Plasmodium falciparum isolates in Thailand using an in vitro human lung endothelial cells model. (ajtmh.org)
  • Using an in vitro model of human lung endothelial cells, we studied different characteristics of Plasmodium falciparum isolates as potential factors for malaria severity in 2 Thai patient groups: 27 with complicated malaria and 42 with uncomplicated malaria. (ajtmh.org)
  • A 3D organotypic model showed that TPSCs stimulated pancreatic cancer cell. (lu.se)
  • A 3D organotypic model showed that TPSCs stimulated pancreatic cancer cell invasion, both as single cell (PANC-1) and cohort (MIAPaCa-2) invasion. (lu.se)
  • A previously validated model based on a spontaneously immortalized rabbit tendon cell line (Teno cell line) was used to evaluate cellular responses to the fluoroquinolones pefloxacin (PEF), ofloxacin (OFX), levofloxacin (LVX), and ciprofloxacin (CIP), in various concentrations. (aspetjournals.org)
  • The Teno cell line may be a useful model for detecting and evaluating tendon toxicity of new fluoroquinolones and other drugs associated with tendinopathy. (aspetjournals.org)
  • Díaz-Prado, S.M. Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells. (medsci.org)
  • Regardless of the model, tumors produced IL-6 and VEGF but not GM-CSF and induced iMC (CD11b(+)Gr-1(int)) that suppressed CTL responses in vitro. (mdc-berlin.de)
  • Muffins containing small (AF) or large (AP) particles of almond were digested in triplicate using an in vitro dynamic gastric model (DGM, 1 h) followed by a static duodenal digestion (8 h). (nuthealth.org)
  • Evaluation of pulmonary effects of 3-D printer emissions from acrylonitrile butadiene styrene using an air-liquid interface model of primary normal human-derived bronchial epithelial cells. (cdc.gov)
  • Nature Cell Biology. (wikipedia.org)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (nih.gov)
  • However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (nih.gov)
  • The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. (nih.gov)
  • The capability of cell-penetrating peptides (CPPs) to transport cargos over different cellular membranes both in vitro and in vivo have drawn major attention in the past decade. (avhandlingar.se)
  • The translocation of CPPs into plant protoplasts might open up a new method for transformation of plant cells.Next, the cell-penetrating ability of the novel peptide YTA2 was characterized and it was established that chemical coupling between YTA2 and the protein cargo is not needed for the transport of the cargo over the cellular membrane in vitro. (avhandlingar.se)
  • This effect is associated with enhanced lipid peroxidation and decreased O 2 consumption by those cells, which are both suggestive of increased cellular stress. (endocrine-abstracts.org)
  • Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
  • It was also shown that IDPs from 45S5.2B BG could enhance in vitro HUVEC tubule formation and secretion of interleukin 6 (IL6) and the basic fibroblast growth factor (bFGF). (rsc.org)
  • The effect of oviductal cells on in vitro maturation of canine oocytes" by SİNEM ÖZLEM ENGİNLER, ASİYE İZEM SANDAL et al. (tubitak.gov.tr)
  • In conclusion, in the oocytes obtained from bitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without oviductal cells, more positive effects were seen on canine oocyte maturation than with TCM-199 medium supplemented with same protein sources and oviductal cells. (tubitak.gov.tr)
  • The caffeine derivatives in mate tea not only induced death in human colon cancer cells, they also reduced important markers of inflammation," said Elvira de Mejia, a U of I associate professor of food chemistry and food toxicology. (sciencedaily.com)
  • However, individual surface markers exhibited a varied relationship to cell division. (unboundmedicine.com)
  • Although changes in some markers (L-selectin) occurred independently of cell division, changes in other markers were either strictly related to cell division (CD45RB) or were a prerequisite to cell division (CD4, CD44). (unboundmedicine.com)
  • The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)
  • After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
  • After co-culture with tumour-associated pancreatic stellate cells (TPSCs), pancreatic cancer cells displayed up to 8-fold reduction in levels of epithelial-mesenchymal transition (EMT) markers E-cadherin and ZO-1, while β-catenin and vimentin levels were increased. (lu.se)
  • High glucose conditions further enhanced changes in EMT markers as well as the cancer cell invasion. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • At 24 hours after the exposure, significant increases in the pro-inflammatory markers IL-12p70, IL-13, IL-15, IFN-y, TNF-a, IL-17A, VEGF, MCP-1, and MIP-1a were noted in the basolateral cell culture medium of ABS-exposed cells compared to non-exposed chamber control cells. (cdc.gov)
  • We describe using this system to quantitatively measure adhesion of the inflammatory cells - monocytic THP-1 cell line to the HUVEC monolayer. (bvsalud.org)
  • A mechanistic simulation engine for the in vitro dissolution experiment that predicts in vitro disintegration and dissolution of pharmaceutical dosage forms. (simulations-plus.com)
  • The integrins' involvement in all of these processes can facilitate the communication between extracellular matrix, inflammatory cells, fibroblasts, and parenchymal cells through their ability to bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands. (harvard.edu)
  • These data indicated that the interactions between lung cancer cells and HBFs in asthmatic bronchi may facilitate the colonization of lung tumors by fibroblasts. (spandidos-publications.com)
  • However, the contribution of asthmatic bronchial fibroblasts in systemic dissemination of lung cancer cells remains unaddressed. (spandidos-publications.com)
  • For cell reprogramming simply provide us with users patient/donor skin fibroblasts, blood cells or iPS cells and we will take care of the rest. (technologynetworks.com)
  • In these experiments, we demonstrated that VZV interferes with NF-kappaB activation in cultured fibroblasts and in differentiated epidermal cells in skin xenografts of SCIDhu mice infected in vivo. (simulations-plus.com)
  • Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
  • LX-2 cells are spontaneously immortalized human hepatic stellate cells and stellate cells are believed to play a role in fibrotic diseases of the liver. (harvard.edu)
  • Micropipette aspiration rheology of cell clusters and freshly isolated mouse tumors revealed viscoelasticity and cell cohesion in both tissues. (aiche.org)
  • During lung cancer progression, the connective tissue of bronchial walls provides the scaffold for developing tumors, forms tissue barriers against immune responses of the host and/or constitutes the routes for the invasion of cancer cells ( 12 ). (spandidos-publications.com)
  • Tumors frequently induce immature myeloid cells (iMC), which suppress specific and unrelated cytotoxic T lymphocyte (CTL) responses and are termed myeloid derived suppressor cells (MDSC). (mdc-berlin.de)
  • A single exposure or multiple exposures of these cells to MNU was effective in inducing tumorigenic cells that produced palpable tumors as early as 6 weeks after transplantation. (eurekamag.com)
  • Background: Pheochromocytomas (PHEO) are rare neuroendocrine tumors derived from chromaffin cells of the adrenal medulla. (endocrine-abstracts.org)
  • The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
  • Stem cell infusion is a relatively simple process that is performed at the bedside. (medscape.com)
  • Integrins are cell adhesion molecules that reside on the surface of cells as transmembrane proteins made of an alpha and beta subunit. (harvard.edu)
  • A tyrosine kinase inhibitor known to induce stress proteins in non cardiac cells was studied in detail. (ucl.ac.uk)
  • The delivery of proteins into cells by mere coincubation with CPPs is an improvement, since the chemical coupling between the CPP and the cargo molecule, which adds time-consuming synthesis and purification steps, can be omitted.Finally, by conjugating each of the two breast tumor homing peptides, the cyclic cCPGPEGAGC (PEGA) or the linear CREKA peptide, to the CPP pVEC, two cell-penetrating peptides with homing properties were obtained. (avhandlingar.se)
  • In a process that was dependent upon the presence of infectious VZV, these proteins rapidly became sequestered in the cytoplasm of VZV-infected cells. (simulations-plus.com)
  • We suggest that VZV has evolved a mechanism to limit host cell antiviral defenses by sequestering NF-kappaB proteins in the cytoplasm, a strategy that appears to be unique among the herpesviruses. (simulations-plus.com)
  • Mammary epithelial cells, isolated from virgin BALB/c mice, were embedded within collagen gels and grown in a serum-free medium containing prolactin, progesterone, and linoleic acid. (eurekamag.com)
  • With established cell culture methods as well as some of our own new ones that use the latest technologies such as 3D cell cultures, bioprinting and microfluidics, we are able to determine the mechanisms of diseases and investigate the effects and side effects of substances. (fhnw.ch)
  • Methods: Fluorescence microscopy confirmed that mTHPC was incorporated into all three cell types. (open.ac.uk)
  • Antibody inhibitors and siRNA were used to manipulate the expression profiles of LX-2 cells that were incubated under normal culture conditions or after treatment with TGF-Β, a known ligand for the αv integrins and a pro-fibrotic cytokine, to mimic a pro-fibrotic environment in vitro. (harvard.edu)
  • Cells were propagated in Earle's minimal essential medium (MEM) (Gibco Invitrogen, Paisley, UK) containing L-glutamine, antimicrobial drugs, and 10% fetal calf serum (FCS). (cdc.gov)
  • The A549 human lung adenocarcinoma and CL1-0 lung adenocarcinoma cell lines were cultured in Dulbecco′s modified eagle medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic antimycotic. (hindawi.com)
  • A variety of primary cells and established cell lines support the replication of flaviviruses: Green monkey (Vero), hamster (BHK-21), human (SW-13, HeLa), porcine (PS), and mosquito cell lines, as well as primary chicken and duck embryo cells have been used for flavivirus isolation and propagation in routine diagnostic applications. (cdc.gov)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • Molecular docking studies indicated that all three complexes primarily bind to HSA in subdomain IIA with amino acid residues such as Arg218 and Lys199 which are located at the entrance of Sudlow's site I. The in vitro cytotoxicities of complexes 1-3 against HeLa cells showed promising anticancer activity (IC 50 = 3.5, 3.9 and 16.9 μM for 1 , 2 and 3 , respectively). (rsc.org)
  • This document is applicable to any molecular in vitro diagnostic examination performed by medical laboratories. (clsi.org)
  • IkappaBalpha levels did not diminish even though the protein became phosphorylated and ubiquitinated, as determined based on detection of the characteristic high-molecular-weight form of the protein, and the 26S proteasome remained functional in VZV-infected cells. (simulations-plus.com)
  • Metabolism of Aedes aegypti cells grown in vitro. (gc.ca)
  • A detailed modeling & simulation program for in vitro metabolism & transport studies. (simulations-plus.com)
  • In our current project we are working on the generation of immortal immune cell lines in order to improve our In vitro Immunization approach. (uni-potsdam.de)
  • The use of such cell lines allows the ex vivo induction of an immune response and makes it independent from experimental animals. (uni-potsdam.de)
  • Thereby the in vitro Immunization represents an alternative to the conventional hybridoma technique because cell lines would provide permanent and limitless numbers of the immune cells needed and could be generated from different host species. (uni-potsdam.de)
  • Cell mediated immune reactions. (crl.edu)
  • T-cell engager (TCE) molecules activate the immune system and direct it to kill tumor cells. (springer.com)
  • The formation of this trimolecular complex (i.e. trimer) mimics the immune synapse, leading to therapeutic-dependent T-cell activation and killing of tumor cells. (springer.com)
  • Together, iMC expand independent of the type of anti-tumor response and are not immune-suppressive in a cell-autonomous fashion. (mdc-berlin.de)
  • Chronic inflammation and tissue fibrosis have long been considered to induce phenotypic shifts in cancer cells, such as epithelial-mesenchymal transition (EMT), which accounts for the formation of cancer invasive front. (spandidos-publications.com)
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
  • Background: The effect of photodynamic therapy (PDT) on neural cells is important when tumours are within or adjacent to the nervous system. (open.ac.uk)
  • These neural cells are derived using integration-free, fully defined neural induction conditions. (technologynetworks.com)
  • AMSBIO neural cells are capable of generating a spectrum of cortical neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. (technologynetworks.com)
  • We can efficiently differentiate the iPS cells (provided by users or generated by us) to cerebral cortical neural lineage using defined neural induction medium and substrates. (technologynetworks.com)
  • 1 In humans, glucocorticoid receptors have been shown in many different cells, including those of the granulocyte-macrophage lineage and lympho cytes. (bmj.com)
  • 2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
  • Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro. (bvsalud.org)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
  • None of the iMC from the different tumor models suppressed CTL responses in adoptive cell transfer experiments, unless GM-CSF was provided in vivo. (mdc-berlin.de)
  • Despite strong interest in these diseases, high quality human in vitro cell models for cortical neurophysiology were unavailable until now. (technologynetworks.com)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • however, viral multiplication was detected in all mammalian cell types by immunohistochemical tests. (cdc.gov)
  • In the local tumour microenvironment, elevated glucose levels, the presence of tumour-associated stellate cells and macrophages are hypothesised to promote the tumour progression and invasion. (lu.se)
  • The key mechanism of action of TCEs is to crosslink CD3 on T cells and tumor associated antigens (TAAs) on tumor cells. (springer.com)
  • We investigated the susceptibility to Usutu virus ( Flavivirus ) of 13 permanent cell lines, 3 primary cell cultures, and chicken embryos. (cdc.gov)
  • We investigated the in vitro susceptibility of various cell cultures and embryonated eggs to Usutu virus infection. (cdc.gov)
  • To 1-day-old confluent monolayers of the permanent cell lines and primary cell cultures, grown on the surface of chamber slides, the Austrian Usutu virus strain Vienna 2001-blackbird (GenBank accession no. (cdc.gov)
  • We employ cell cultures and tissue engineering in our research in order to investigate diseases and the efficacy and toxicity of substances. (fhnw.ch)
  • NA HBFs did not significantly affect A549 motility, whereas AS HBFs and the media conditioned with AS HBF/A549 co‑cultures increased Snail‑1/connexin43 expression and motility of A549 cells. (spandidos-publications.com)
  • Conclusion The conclusion was reached that using risedronate, cobalamin and both drugs in combination on osteoblastic cell cultures does not cause alterations to their growth or in the formation of calcium and phosphate nodules. (bvsalud.org)
  • The malformations have a normal endothelial cell growth cycle that affects the veins, the capillaries, or the lymphatics, and they do not involute. (medscape.com)
  • Recurrence and chemoresistance in pancreatic cancer are largely related to cancer stem cells. (isciii.es)
  • Conclusion: Neurons in culture can survive mTHPC-PDT under conditions sufficient to kill tumour cells and other nervous system cells. (open.ac.uk)
  • For erythroid colony forming unit (CFU-E) quantification, we used 2000 cells/ml, while for erythroid burst forming units (BFU-E), colony forming units mix (CFU-mix), and granulocyte-macrophage colony-forming unit's (CFU-GM), we used 7000 cells/ml. (bmj.com)