• The molecules used in the in vitro test do not cross the blood-brain barrier. (scienceboard.net)
  • ZK 219477 has the ability to cross the blood-brain barrier, a fundamental characteristic for a potential GBM chemotherapy. (knowcancer.com)
  • Our in vitro and in vivo studies indicate the potential of these drugs as candidates for the treatment of glioblastoma especially since they can cross the blood-brain barrier. (edu.au)
  • The possibility of using genes as medicines to treat brain diseases is currently limited by the lack of safe and efficacious delivery systems able to cross the blood-brain barrier, thus resulting in a failure to reach the brain after intravenous administration. (nih.gov)
  • Examining the ways in which the West Nile virus may cross the blood-brain barrier to infect the nervous system, Verma et al infected primary human brain microvascular endothelial (HBMVE) cells with NY99, a neurovirulent strain of the virus. (medscape.com)
  • Fluids and barriers of the CNS , 17(1), article no. 51. (open.ac.uk)
  • Their paper, ' Evaluation of the influence of astrocytes on in vitro blood-brain barrier models ', was published in ATLA 48(4), 184-200. (frame.org.uk)
  • The lead author recently provided ATLA with an update on the project: "In the submitted paper, we set up and characterised 20 different BBB in vitro models, based on the combination of epithelial or endothelial cell lines and primary or immortalised astrocytes/ glial cells or their derivatives. (frame.org.uk)
  • Our strategy is to investigate the effect of AD risk factors like stress, inflammation, metabolic changes, and amyloidogenesis, on different types of brain cells including pericytes, astrocytes, neurons, and NG2 cells. (lu.se)
  • Aprotinin transcytosis across bovine brain capillary endothelial cell (BBCEC) monolayers is at least 10-fold greater than that of holo-transferrin. (aspetjournals.org)
  • Our research group continues its work on the development and improvement of in vitro models of the BBB, levelling up to a tri-culture model. (frame.org.uk)
  • For this aim, the group has selected different cell lines and primary cultures of microvascular, perivascular endothelial and astroglia cells, to perform and compare different in vitro BBB models to select the most reliable model. (frame.org.uk)
  • In vitro blood-brain barrier model for pre-clinical assessment of food/drug compounds. (dcu.ie)
  • A hydrogel model of the human blood-brain barrier using differentiated stem cells. (open.ac.uk)
  • A human-derived neurovascular unit in vitro model to study the effects of cellular cross-talk and soluble factors on barrier integrity. (open.ac.uk)
  • Using an innovative in vitro model of the blood-brain barrier, Dennis Grab of Johns Hopkins University and his colleagues found that Trypanosoma brucei gambiense requires calcium ions and cysteine proteases to migrate across.1 "This is a big technical and scientific advance," says Paul Roepe of Georgetown University. (the-scientist.com)
  • We have the beginnings of a real molecular model for how trypanosomes are crossing the blood-brain barrier. (the-scientist.com)
  • Using an innovative in vitro model of the blood-brain barrier, Dennis Grab of Johns Hopkins University and his colleagues found that Trypanosoma brucei gambiense requires calcium ions and cysteine proteases to migrate across. (the-scientist.com)
  • In pre-clinical model ZK 219477 has demonstrated anti-tumorigenic activity for gliomas tumours both in vitro and in vivo. (knowcancer.com)
  • Cytotoxicity and apoptotic gene expression in an in vitro model of the blood-brain barrier following exposure to poly(butylcyanoacrylate) nanoparticles. (neurotree.org)
  • Analysis of biotinylated generation 4 poly(amidoamine) (PAMAM) dendrimer distribution in the rat brain and toxicity in a cellular model of the blood-brain barrier. (neurotree.org)
  • Quinolinic acid released from polymeric brain implants causes behavioral and neuroanatomical alterations in a rodent model of Huntington's disease. (neurotree.org)
  • We demonstrate here the transcytosis ability of aprotinin and peptides derived from Kunitz domains using an in vitro model of the BBB and in situ brain perfusion. (aspetjournals.org)
  • The present study was implemented to develop a novel, translational in vitro model to assess systemic inflammatory changes caused by inhaled ENMs. (cdc.gov)
  • Using a preterm rabbit pup model, we investigated the uptake of systemic recombinant human (rh) IGF-1 in complex with its main binding protein IGF-binding protein 3 (BP-3) to the brain parenchyma via the choroid plexus. (lu.se)
  • In vitro studies of the metabolism of atrazine, simazine, and terbutryn in several vertebrate species. (cdc.gov)
  • 1993. In vitro percutaneous absorption and metabolism in man of 2-chloro-4-ethylamino-6-isopropylamine-s-triazine (atrazine). (cdc.gov)
  • Cholesterol metabolism in the brain is independent of peripheral tissues due to the blood-brain barrier (BBB) that impairs the entrance of the protein-bound lipid into the central nervous system (CNS). (frontiersin.org)
  • Brain tissues contain large amounts of cholesterol, up to 25% of the body's cholesterol content ( Dietschy, 2009 ) whose metabolism and complex homeostasis regulation in the CNS remain unclear ( Zhang and Liu, 2015 ). (frontiersin.org)
  • In this review we recorded several representative in vitro and in vivo studies performed in culture cell lines, in animal or clinical trials, indicating that the regular intake of EVOO is associated with enhanced neuroprotective, antioxidant, anti-inflammatory, anti-atherosclerotic, anti-cancer and anti-microbial properties. (researchgate.net)
  • For example, a class of neurotransmitters called endocannabinoids bind to proteins called cannabinoid receptors in the BBB, and the receptors help transport the molecules across the barrier and into the brain. (sciencedaily.com)
  • An in vitro test found the combination of dasatinib and quercetin, molecules that remove senescent cells in conditions such as osteoarthritis, returned the number of senescent neurons to normal levels. (scienceboard.net)
  • In vitro, serum from MWCNT exposed mice was able to induce expression of adhesion molecules at both the gene and protein level. (cdc.gov)
  • In 2015, Dr. Qiang returned to Drexel University, where he now leads a research laboratory that focuses on using human induced pluripotent stem cell (hiPSC) based technologies to develop in vitro and ex vivo cellular and tissue models. (drexel.edu)
  • Prion diseases result from misfolding of a normal cell-surface brain protein called cellular prion protein (PrP C ), whose exact function is unknown. (msdmanuals.com)
  • As a parameter to describe the in vivo P-gp function, we calculated the brain-to-plasma concentration ratio of compounds in mdr1a/1b knockout mice divided by the same ratio in wild type mice. (nih.gov)
  • An alcohol-free fermented blueberry and blackberry beverage extract attenuates diet-induced obesity and blood glucose in mice. (greenmedinfo.com)
  • Ana Torres-Suárez and colleagues wanted to make use of this system to sneak drug nanocarriers into the brains of mice. (sciencedaily.com)
  • Similarly, when injected into healthy mice, the CBD-nanocapsules targeted about 2.5 times more of the fluorescent molecule to the animals' brains. (sciencedaily.com)
  • Loss of cholinergic innervation differentially affects eNOS-mediated blood flow, drainage of Aβ and cerebral amyloid angiopathy in the cortex and hippocampus of adult mice. (open.ac.uk)
  • Quantitative targeted absolute proteomic analysis of 31 membrane proteins showed that P-glycoprotein (P-gp/mdr1a) protein expression levels were significantly increased in brain capillaries of PTZ (129%), EL (143%), and PHT mice (192%) compared with controls. (aspetjournals.org)
  • In all mice, reconstructed K p brain values agreed well with the observed values within a 1.21-fold range. (aspetjournals.org)
  • In a preclinical PK study, similar concentrations of ZK 219477 were detected in plasma (1.2 μg/ml) and brain (0.9 μg/g) after 10 min after i.v. application in scid mice. (knowcancer.com)
  • In vitro, the conjugation of transferrin to the polypropylenimine dendrimer increased the DNA uptake by bEnd.3 murine brain endothelioma cells overexpressing transferrin receptors, by about 1.4-fold and 2.3-fold compared to that observed with the non-targeted dendriplex and naked DNA. (nih.gov)
  • MWCNT exposure impaired blood brain barrier integrity, as noted by fluorescein uptake, which was associated with increased gene expression of IL-6 and CCL5 in the cortex and hippocampus. (cdc.gov)
  • Pretreatment with the rho kinase inhibitor, fasudil, was able to prevent brain fluorescein uptake and neuroinflammation. (cdc.gov)
  • Here, we characterize the uptake of systemic rhIGF-1/rhIGFBP-3 to the preterm brain, and show that the interaction between systemic rhIGF-1/rhIGFBP-3 and choroid plexus varies over time. (lu.se)
  • The BBB is the biggest limiting hurdle to deliver a drug to the brain, and this is especially true in the case of gene delivery. (sbir.gov)
  • The current mode of administration that is typically used for brain delivery for gene therapy is the highly invasive intrathecal administration, while other methods such as intravenous injection would provide less physical burden on the patients. (sbir.gov)
  • By engineering new delivery vehicles using novel biomaterials or delivery modalities, gene therapy can have enhanced stability and bioavailability, decreased immunogenicity, increased brain delivery, and improved cell-type targeting. (sbir.gov)
  • Second, the team looked at senescence markers and gene expression in post-mortem brains of 20 people with Alzheimer's and matched healthy controls. (scienceboard.net)
  • In vivo, the intravenous injection of transferrin-bearing dendriplex more than doubled the gene expression in the brain compared to the unmodified dendriplex, while decreasing the non-specific gene expression in the lung. (nih.gov)
  • Gene expression was at least 3-fold higher in the brain than in any tested peripheral organs and was at its highest 24h following the injection of the treatments. (nih.gov)
  • These results suggest that transferrin-bearing polypropylenimine dendrimer is a highly promising gene delivery system to the brain. (nih.gov)
  • Moreover, using choroid plexus in vitro cell cultures, gene expression and protein synthesis were further investigated upon rhIGF-1/rhIGFBP-3 stimulation as compared to rhIGF-1 alone, and found not to be differently altered. (lu.se)
  • The purpose of the present study was to establish in vitro models to predict the in vivo function of P-gp. (nih.gov)
  • The value of in vitro models of the blood-brain barrier and their uses. (edgehill.ac.uk)
  • Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
  • The research group specialises in the development of in vitro models of different physiological barriers, such as the blood-brain barrier (BBB), pulmonary barrier and intestinal barrier, with a clear orientation toward the development of robust in vitro models according to the 3Rs principles. (frame.org.uk)
  • The results obtained with in vitro and in vivo models provided evidence for greater passage of aprotinin across the BBB than of holo-transferrin. (aspetjournals.org)
  • As an in vitro method, the transcellular transport of 12 compounds across the monolayer of Caco-2- and MDR1-transfected cells was examined. (nih.gov)
  • Collectively, the in vitro flux ratio across monolayers of P-gp-expressing cells may be used to predict in vivo P-gp function. (nih.gov)
  • This can involve movement through the circulatory system and further through cells of the blood-brain-barrier (BBB). (sbir.gov)
  • The BBB consists of a layer of tightly linked cells that line capillaries in the brain, preventing substances from exiting the blood and entering the brain. (sciencedaily.com)
  • In experiments with human brain cells that mimic the BBB, the researchers showed that the CBD-displaying nanocarriers caused more of the fluorescent molecule to pass through the cells than nanocarriers of equal size that lacked CBD. (sciencedaily.com)
  • A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. (worthington-biochem.com)
  • Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. (worthington-biochem.com)
  • Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. (worthington-biochem.com)
  • The tetra(ethylene glycol) derivative of benzothiazole aniline, BTA-EG 4 , is a novel amyloid-binding small molecule that can penetrate the blood-brain barrier and protect cells from Aβ-induced toxicity. (jneurosci.org)
  • Blood vessels (BVs) are considered an integral component of neural stem cells (NSCs) niches. (elifesciences.org)
  • Neural stem cells (NSCs) serve as a source for new neurons in both the developing and adult brain. (elifesciences.org)
  • A good correlation was observed between the in vitro flux ratio across the monolayer and in vivo P-gp function for 12 compounds. (nih.gov)
  • With regard to human biomonitoring, analysis of organochlorine compounds and metals in biological samples (blood, brain, milk) in venous serum of pregnant mothers from Arctic areas, farmworkers exposure to pesticides in Spain, phthalates and DINCH metabolites, bisphenols, parabens, and triclosan in Slovenian populations, and metals in from population samples from the PHIME study has been carried out. (europa.eu)
  • My research interests include hypoxia biology, stroke medicine and blood-brain barriers. (keele.ac.uk)
  • Furthermore, I am interested in neuroinflammation in hypoxia and investigating the effects of microglial activation on blood-brain barrier functions in hypoxia. (keele.ac.uk)
  • Upon brain hypoxia, pericytes are one of the first responders at the blood/brain interface. (lu.se)
  • Enstrom A, Carlsson R, Ozen I, and Paul G. RGS5: a novel role as a hypoxia-responsive protein that suppresses chemokinetic and chemotactic migration in brain pericytes. (lu.se)
  • Overall, these results suggest that these Kunitz-derived peptides could be advantageously used as a new brain delivery system for pharmacological agents that do not readily enter the brain. (aspetjournals.org)
  • Understand the role of amyloid peptides (including amyloid-beta, IAPP, TDP-43) in blood-brain barrier (BBB) alterations and how it affects the progression of AD. (lu.se)
  • We aim to fulfil Multiparks goals by exploring novel AD mechanisms and investigating how aggregated amyloid peptides affect brain function and cell integrity. (lu.se)
  • High-grade gliomas are primary brain cancers with unacceptably low and persistent survival rates of 10-16 months for WHO grade 4 gliomas over the last 40 years, despite surgical resection and DNA-damaging chemo-radiotherapy. (nature.com)
  • The oppression and destruction by hematoma to brain tissue cause the primary brain injury. (karger.com)
  • In the first few hours after ICH onset, primary brain injury by ICH is mainly caused by the oppression and destruction to the near tissue by hematoma formation. (karger.com)
  • Brain edema is a pathological phenomenon that water and brain tissue volume increase. (karger.com)
  • Our work also spans from human brain tissue and cohort studies to in vitro and animal studies which is line with Multiparks translational initiative. (lu.se)
  • The purpose of this study was to demonstrate experimentally that alterations of in vivo transporter function at the blood-brain barrier (BBB) in disease and during pharmacotherapy can be reconstructed from in vitro data based on our established pharmacoproteomic concept of reconstructing in vivo function by integrating intrinsic transport activity per transporter molecule and absolute protein expression level at the BBB. (aspetjournals.org)
  • At 7wk, HF-fed animals exhibited several immune alterations (blood leukocyte/neutrophil number, lymph node B-cell proportionality)-effects which were more pronounced in SD rats. (cdc.gov)
  • The blood-brain barrier (BBB) plays an important role in regulating the exchange of nutrients and wastes between brain tissues and the circulatory system while concurrently preventing pathogens from entering the brain parenchyma. (nature.com)
  • Because hippocampal BVs (including those of the DG where RGLs reside) are thought to have a fully-functional BBB, the question arises how are circulating substances accessible to the brain parenchyma without a loss of BBB function? (elifesciences.org)
  • Brain capillaries also possess few fenestrae and few endocytic vesicles, compared with the capillaries of other organs ( Pardridge, 1999 ). (aspetjournals.org)
  • The development of the human blood-CSF-brain barrier. (cdc.gov)
  • Particular attention was given to the distribution and expression of sEH in the human brain, deepening into the enzyme's phosphatase activity and its participation in brain cholesterol synthesis. (frontiersin.org)
  • We also provide a compilation of sEH distribution and expression in the human brain and deepen the discussion on the enzyme's N-terminal phosphatase activity and its involvement in the synthesis of cholesterol. (frontiersin.org)
  • Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. (worthington-biochem.com)
  • A quantitative understanding of how the molecular transport systems at the human BBB are altered in diseases is essential not only to elucidate the pathophysiological roles of the BBB in human brain, but also to aid development of effective drugs and appropriate pharmacotherapies for CNS diseases. (aspetjournals.org)
  • These are followed up by in vitro experiments and studies on human CSF and plasma. (lu.se)
  • Other in vitro studies have shown bilirubin to have more antioxidant capability than vitamin E, which is commonly assumed to be the most potent antioxidant in the human system. (medscape.com)
  • This study cites many factors which are causing the Global In-vitro Diagnostic Services Market to expand rapidly. (medgadget.com)
  • Some of the key factors analyzed in the study include the impact of global population growth, burgeoning technological advancements and the demand and supply dynamics noted on the Global In-vitro Diagnostic Services Market. (medgadget.com)
  • So, it is very important to study on the relationship between brain edema and ICH. (karger.com)
  • We have established a method allowing to study the cell-specific secretome of pericytes in vitro-using Turbo-ID, a mutant non-toxic biotin ligase (6). (lu.se)
  • LFLB1, DJOA2, VCFR3: study aumenta la posibilidad de desarrollar esquizofrenia y depresión en individuos sin historial previo, y que podría exacerbar conception, VCFR3: manuscript cuadros psiquiátricos previos con dificultad en el tratamiento. (bvsalud.org)
  • Consequently, it plays an important role in maintaining brain homeostasis and in restricting the delivery of many therapeutic and diagnostic drugs to the brain. (aspetjournals.org)
  • My PhD was focused on investigating the effects of antipsychotic drugs and statins in the treatment of glioblastoma multiforme, an aggressive brain cancer. (edu.au)
  • Because most drugs do not cross the BBB, few treatments are available against most CNS disorders, including Parkinson's disease, Alzheimer's disease, and brain cancers (Pardridge, 2001 , 2005 ). (aspetjournals.org)
  • Given that brain cholesterol disturbances play a role in the onset of Alzheimer's disease (AD) as well as of other neurodegenerative diseases, understanding the functions of this enzyme could provide pivotal information on the pathophysiology of these conditions. (frontiersin.org)
  • Through the analyses, the researchers showed that the brains of Alzheimer's patients have significantly higher proportions of neurons that express senescence markers, and that their distribution indicates bystander effects. (scienceboard.net)
  • Alzheimer's disease (AD) is a neurodegenerative disease characterized by aggregated extracellular amyloid-β (Aβ) plaques in the brain ( Finder and Glockshuber, 2007 ). (jneurosci.org)
  • On the basis that iron can effectively reach the brain by using transferrin receptors for crossing the blood-brain barrier, we propose to investigate if a transferrin-bearing generation 3-polypropylenimine dendrimer would allow the transport of plasmid DNA to the brain after intravenous administration. (nih.gov)
  • Different transporters and receptors present at the BBB have been described as playing roles in maintaining the integrity of the BBB and brain homeostasis. (aspetjournals.org)
  • There, he played a vital role in establishing an in vitro platform of the blood-brain barrier for drug screenings in the central nervous system, as well as a CNS-derived microvasculature environment to support neuronal reprogramming. (drexel.edu)
  • Bilirubin staining of the brain without accompanying neuronal necrosis can be observed in babies who did not demonstrate clinical signs of bilirubin encephalopathy but who succumbed from other causes. (medscape.com)
  • Brain pericytes acquire a microglial phenotype after stroke. (lu.se)
  • Footnotes *Testing of whole blood lead level is the most sensitive and specific means in assessing lead toxicity. (aetna.com)
  • vasogenic factors, thrombin formation, erythrocyte lysis and hemoglobin (Hb) toxicity [ 5 ] have been proved to be related to brain edema growth [ 6 ]. (karger.com)
  • CBD seems to have effects on some chemicals in the brain, but these are different than the effects of THC. (medlineplus.gov)
  • ZK 219477 demonstrated both in vitro and in vivo considerable anti-tumour activity against a wide range of malignancies, and it is now about to enter phase II studies in a number of selected indications. (knowcancer.com)
  • In recent years, many studies focus on the mechanism of secondary inflammation that can cause brain edema and this may provide new therapy targets for ICH [ 7 ]. (karger.com)
  • However, for most BBB transporters, it is still difficult to synthesize selective probes and inhibitors to enable specific evaluation of the transport activities of the target transporters, because of the effects of other transporters, including functionally unknown transporters, as well as binding to brain tissues. (aspetjournals.org)
  • Improved brain imaging modalities, such as MRI and ultrasonography, may be emerging as instrumental tools to help clarify the complex picture of kernicterus in contrast with asymptomatic bilirubin staining of brain tissues. (medscape.com)
  • Insulin-like growth factor-1 (IGF-1) is essential for normal brain development and regulates essential processes of vascular maturation and stabilization. (lu.se)
  • Many AAV serotypes have been developed using capsid modification strategies, but each serotype has various limitations associated with brain delivery, cell-type targeting, immunogenicity, biodistribution, and payload size. (sbir.gov)
  • This is characterized by a marked increase in fecal urobilinogen excretion and a normal or near-normal red blood cell lifespan. (medscape.com)
  • They operate during sensitive periods of gestation by disrupting endocrine systems and altering the hormonal milieu necessary for fetal brain development. (europa.eu)
  • A narrative bibliographic review article was done with the search of original and review articles in international scientific mentales en adultos journals, in English and Spanish listing the relationship between the seroprevalence of T. gondii and the development of mental disorders in the adult population. (bvsalud.org)
  • Deltorphin possesses very high affinity and selectivity as an agonist for the δ-opioid receptor, and on account of its unusually high blood-brain-barrier penetration rate, produces centrally-mediated analgesic effects in animals even when administered peripherally. (wikipedia.org)
  • Because the blood-brain barrier limits penetration of penicillin into the cerebrospinal fluid (CSF), no intermediate category for meningitis exists. (cdc.gov)
  • NT008 trade name] is indicated in adults and children for the elimination through mass drug administration programmes of schistosoma infections due to various types of blood fluke worms ( Schistosoma mansoni, Schistosoma haematobium, Schistosoma japonicum, Schistosoma mekongi, Schistosoma intercalatum ) following the recommendations of the WHO Global Programme to Eliminate Schistosomiasis. (who.int)
  • Comprehensive research methodology of Global In-vitro Diagnostic Services Market. (medgadget.com)
  • Although much more research is needed to verify these claims, scientists have now shown that CBD could have a different use as a 'Trojan horse': helping slip medications across the blood-brain barrier (BBB) and into mouse brains. (sciencedaily.com)