Vitro b lymphocytes. Medical search. Frequent questions

Synergy among thymocytes and peripheral lymph node cells in the in vitro generation of lymphocytes cytotoxic to alloantigens. (ox.ac.uk)
In this study, possible genotoxic effects of zinc oxide (ZnO) nanoparticles were investigated in cultured human peripheral lymphocytes by using chromosome aberrations and micronucleus assays (MN). (comu.edu.tr)
Peripheral blood lymphocytes were irradiated in vitro with 213 Bi α particles at doses of 0, 10, 20, 50, 100, 200 and 500 mGy. (bioone.org)
A linear dose response for genomic frequencies of translocations in stable cells fitted the data from 0 to 200 mGy with a dose response of 7.90 ± 0.98 × 10 −2 per Gy, thus indicating that they are likely to be observed in peripheral blood lymphocytes from individuals with past or chronic exposure to high-LET radiation. (bioone.org)
Consequently, careful sample preparation is of paramount importance if the Comet assay is to be successfully used to assess DNA damage in human peripheral blood lymphocytes. (elsevierpure.com)
We transfected human and rabbit peripheral blond mononaclear cells (PBMC) with the ACH molecular clone of human T-cell lymphotropic virus type 1 (HTLV-1) to study its in vitro and in vivo properties. (wustl.edu)
Cultured peripheral human lymphocytes were used as test system. (europa.eu)
Peripheral human lymphocytes are recommended in international guidelines (OECD, EC). (europa.eu)
Chromosomal aberrations were induced in peripheral blood lymphocytes of workers exposed to vinyl chloride at levels of 5-500 ppm (13-1300 mg/m 3 ). (inchem.org)
The LSM 12.125 antibody reacts with the canine form of the 56 kDa transmembrane glycoprotein, CD4, present on the T-helper/inducer subset of normal canine donor peripheral blood lymphocytes. (bdbiosciences.com)
The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes. (bdbiosciences.com)

While in vitro administration of glucocorticoids may inhibit concanavalin A (Con A)- and phytohemagglutinin (PHA)-induced T-cell proliferation, pokeweed mitogen (PWM)-driven B-cell mitogenesis is relatively resistant to glucocorticoids. (karger.com)
To further explore the link between the HPA and the immune system in relation to glucocorticoid receptor function, dose-response curves were obtained for Con A-and PHA-induced T-cell mitogenesis, PWM-generated B-cell mitogenesis and spontaneous lymphocyte proliferation in 13 healthy controls. (karger.com)
There was a significant decrease in PWM-induced B-cell mitogenesis and a more pronounced effect of DEX administered in vitro on spontaneous lymphocyte proliferation after MET treatment when compared with the DEX plus MET pretreated condition in vivo. (karger.com)
These data suggest that the inhibition of spontaneous lymphocyte proliferation by glucocorticoids in vitro is related to glucocorticoid receptor function. (karger.com)
In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: studies of proliferation, apoptosis and mitochondrial membrane potential med. (emf-portal.org)
The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)
Effects of diacetyl diamines on in vitro activation and proliferation of human B lymphocytes. (aai.org)
Effect of targeted erythrocytes coated with recombinant human interleukin 2 on T-lymphocyte proliferation in vitro. (tamu.edu)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

It has been found that neonatal thymectomy substantially eliminates both PHA reactivity and responsiveness to alloantigens assayed in vitro in host spleen cell populations. (silverchair.com)
Reconstitution of thymic-dependent in vitro reactivity was nearly complete when syngeneic, but not allogeneic or semisyngeneic thymus was employed. (silverchair.com)
The distribution on lymphocytes for canine is similar to that found for both human and monkey, with the majority of CD4-positive lymphocytes being CD8-negative and lacking reactivity with antibodies to B- or NK-cell markers. (bdbiosciences.com)

This study on the in vitro micronucleus assay, comprising 11 laboratories using human lymphocytes, was coordinated by an organizing committee supported by the SFTG (the French branch of the European Environmental Mutagen Society). (maastrichtuniversity.nl)

This book discusses the mechanisms of transmembrane signal transduction during B-Cell Activation, the Regulation of Gene Expression during B-Cell Differentiation, Anti-immunoglobulin antibody induction of B-Lymphocyte activation and differentiation in vivo and in vitro, Helper signals in B-Lymphocyte differentiation, Growth and differentiation of b-cell clones in vitro, B-Cell tumors as models of lymphocyte differentiation, and Memory b-cell activation, differentiation, and isotype switching. (taylorfrancis.com)

Đelić N, Potparević B, Živković L, Marković B, Dačić S. In vitro analysis of clastogenic effects of adrenaline on human lymphocytes. (ac.rs)
N,N'-Diacetylputrescine (tetramethylenebisacetamide [TMBA]) and its six carbon analog, hexamethylenebisacetamide (HMBA), inhibited the proliferative response of human B lymphocytes to anti-mu and formalinized Cowan I strain Staphylococcal aureus (SAC) stimulation. (aai.org)
These studies show that diacetylated derivatives of polyamines modulate human B cell activation in vitro by specific abrogation of anti-mu or SAC activation. (aai.org)
In vitro Safety Evaluation and Anticlastogenic Effect of BacoMindTM on Human Lymphocytes[J]. Biomedical and Environmental Sciences, 2008, 21(1): 7-23. (besjournal.com)
The present study aimed to investigate the in vitro toxicity of this formulation of BacoMindTM on human lymphocytes and to rule out its possible contribution to mutagenicity. (besjournal.com)
The present results indicate that ZnO nanoparticles are clastogenic, mutagenic and cytotoxic to human lymphocytes in vitro at specific concentrations and time periods. (comu.edu.tr)
Nine coded substances were assessed for their ability to induce micronuclei in human lymphocytes in vitro, mitomycin C being used as a positive control. (maastrichtuniversity.nl)
CMV infection has been shown to induce transient abnormalities of in vitro cellular-immune function in otherwise healthy human hosts (2,3). (cdc.gov)
Vinyl chloride induced sister chromatid exchanges in human lymphocytes in vitro . (inchem.org)
EBV infects human B-lymphocytes and is the etiological agent of infectious mononucleosis. (pennmedicine.org)
Both belong to the oncovirus subfamily of retroviruses and can transform human lymphocytes so that they are self-sustaining in vitro. (cdc.gov)
Human primary tumor cells could also be reprogrammed to increase their capability to present antigen and to activate patient-specific tumor-infiltrating lymphocytes. (lu.se)

In vitro selection and extended culture of antigen-specific T lymphocytes. (rupress.org)
Specific selection of antigen-responsive guinea pig peritoneal exudate lymphocytes (PELs) was achieved by a selection culture procedure. (rupress.org)
after 1 wk of culture, lymphocytes were obtained which were highly responsive to the antigen for which they were selected but which demonstrated little or no response to other antigens to which the original donor of the lymphocyte was immune. (rupress.org)
These selected cells were largely T lymphocytes and could be maintained in culture for 2-5 wk in an antigen-responsive state and, in 20-30% of cases, for 8-10 wk in an antigen-independent state. (rupress.org)
These antigen-presenting cells (APC) were then used to stimulate enriched populations of naive T cells in microcultures for 5 days in vitro. (duke.edu)
however, response to in vitro exposure to candidal antigen is absent. (medscape.com)
In addition to acquiring improved antigen presentation, tumor-APCs had impaired tumorigenicity in vitro and in vivo. (lu.se)

In vitro genotoxic effects of ZnO nanoma. (comu.edu.tr)

The inflammagenic potential of the tested CNP was found to be as follows: GO>C60-TRIS>C60, as evidenced by accumulation of PMNs, macrophages and lymphocytes as well as changes in lung permeability and inflammatory cytokine profiles in the lungs on days 1 and 7 post exposure. (cdc.gov)

These data suggest that ACH, like wild-type HTLV-1, infects and transforms primary CD4 + T lymphocytes and is infectious in vivo. (wustl.edu)
In vitro EBV efficiently growth transforms primary B-lymphocytes. (pennmedicine.org)

Three patients had profoundly depressed in vitro proliferative responses to mitogens and antigens. (cdc.gov)

Other T lymphocytes (ie, T-suppressor/cytotoxic cells) possess the killing function and also inhibit B-cell development. (medscape.com)
The CD8α and β chains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and a subpopuplation of mature T-lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). (bdbiosciences.com)

Cultured lymphocytes with PSK at 100 micrograms/ml increased the level of DNA synthesis, as determined by the 3H-thymidine uptake, from 0.9 to 3.0 fold, compared to the PSK non-treated cells. (unboundmedicine.com)
Glucocorticoid effects were assessed in vivo by depletion of endogenous glucocorticoids after oral administration of 1.5 g metyrapone (MET) and subsequent glucocorticoid replacement, and in vitro by incubation of the cells with different doses of dexamethasone (DEX). (karger.com)
The first stages of the pathway by which lymphocytes differentiate from hemopoietic stem cells were studied at a clonal level. (elsevierpure.com)
The lymphoid cells were proved to be B lymphocytes by phenotype and immunoglobulin gene rearrangement analyses and by demonstration of surface expression of IgM. (elsevierpure.com)
The clonal origin of myeloid and B lymphocyte lineage cells was further confirmed by the generation of both myeloid and B lymphoid cells in the same well following FACS clone-sorting of IL-3 induced blast cells. (elsevierpure.com)
These results provide in vitro evidence that cells of B lymphoid and myeloid lineage can originate clonally from single primitive hemopoietic stem cells. (elsevierpure.com)
Employing cell fractionation with the aid of peanut agglutinin, it was found that at least two subpopulations of T cells are required for the generation of suppressor T lymphocytes in culture. (tau.ac.il)
With the aid of genetic markers, it was found that the medullary-type, cortisone-resistant, PNA-negative T cell was the progenitor of the suppressor lymphocytes, while the cortical type cells performed an accessory (regulatory? (tau.ac.il)
The generation of 'helper' lymphocytes requires allostimulation for less than 24 hr while the induction of supressor cells requires a longer period of stimulation which leads in the end to predominance of the suppressor effect. (tau.ac.il)
This indicates that there are components in whole blood that can cause DNA damage in lymphocytes, with granulocytes and lysis of red blood cells likely candidates. (elsevierpure.com)
RBC were covalently coupled to specific monoclonal antibody (mAb) towards the lymphocyte cell-surface marker Thyl.2, coated with rIL 2, subsequently exposed to the target CTLL cells, then evaluated for CTLL/RBC rosette formation. (tamu.edu)
A panel of DAbR1-expressing T cells and CAR T cells exhibited greater than 8-fold increased uptake of 86 Y-AABD in vitro when compared with nontransduced cells. (snmjournals.org)
The immune system's lymphocyte component is divided into B cells and T cells. (medscape.com)
Traditionally, B cells have been believed to be the lymphocytes responsible for antibody production via maturation into plasma cells (ie, humoral immunity), and T cells have been believed to be the lymphocytes responsible for killing other cells or organisms (ie, cellular immunity). (medscape.com)
Currently, certain T lymphocytes (ie, T-helper cells) are known to be responsible for helping immature B cells develop into mature B cells. (medscape.com)
In this paper, we review the current literature on both in vitro and in vivo studies on the role of the immune system in fracture repair, the use of MSCs in the enhancement of fracture healing, and interactions between MSCs and immune cells. (hindawi.com)
It induced mutation in Chinese hamster cells and unscheduled DNA synthesis in rat hepatocytes in vitro and induced transformation in BALB/c 3T3 cells and virus-infected Syrian hamster cells. (inchem.org)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
Immunized T-lymphocytes which can directly destroy appropriate target cells. (bvsalud.org)

Further, GO and fullerenes were found to induce reactive oxygen species production by RAW 264.7 macrophages in vitro. (cdc.gov)

Antibody formation initiated in vitro. (wikidata.org)

Vaccines which successfully protect against virus infections usually need to induce a broadly reactive immune response which includes the induction of cytotoxic T lymphocytes (CTL). (duke.edu)
Tregs are anergic CD4 + CD25 + Foxp3 + T lymphocytes exerting active suppression to control immune and autoimmune responses. (urosario.edu.co)
The aim of this study was to investigate whether DAbR1 can be expressed on lymphocytes and used as a reporter gene as well as a suicide gene for therapy of immune-related adverse effects. (snmjournals.org)
The adaptive immune response, primarily comprised of T and B lymphocytes, has important implications in the fracture healing process as well [ 3 , 4 ]. (hindawi.com)

It is concluded that the clastogenic or aneugenic potential in vitro of the substances tested was correctly identified in this study, but that refining the protocol to take into account factors such as the stages of the cell cycle exposed to the compound, or the duration of recovery would be likely to improve the sensitivity of detection using lymphocytes. (maastrichtuniversity.nl)

Our findings thus show that the in vitro activation of lymphocytes by PSK can help identify the candidates with either gastric or colorectal cancer who are the best suited to undergo immunochemotherapy including treatment with PSK. (unboundmedicine.com)
Lymphocytes (0.4 mL blood of a healthy male donor was added to 5 mL or 4.8 mL culture medium, without and with metabolic activation respectively and 0.1 mL (9 mg/mL) Phytohaemagglutinin) were cultured for 48 h and thereafter exposed to selected doses of test substance for 3 h, 24 h and 48 h in the absence of S9-mix or for 3 h in the presence of S9-mix. (europa.eu)

This transformant culture was shown by flow cytometric analysis to be a CD4 + CD25 + T-lymphocyte population containing, as determined by Southern blot analysis, at least three integrated HTLV-1 proviral copies. (wustl.edu)

The effects of thymectomy and thymus graft restoration upon the in vitro primary responses to alloantigens and PHA have been studied. (silverchair.com)

Scholars@Duke publication: Induction in vitro of primary cytotoxic T-lymphocyte responses with DNA encoding herpes simplex virus proteins. (duke.edu)

MART-1 is an Ag expressed on melanomas and melanocytes, and is recognized by the majority of HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes (TIL) from melanoma patients. (elsevierpure.com)
Epigenetic silencing of tumor suppressor genes during in vitro Epstein-Barr virus infection. (pennmedicine.org)
Injection of in vitro generated melanoma-derived tumor-APCs into subcutaneous melanoma tumors delayed tumor growth and increased survival in mice. (lu.se)
These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. (bvsalud.org)

In this study, we have used a convenient in vitro approach to investigate if plasmid DNAs encoding proteins of herpes simplex virus (HSV) are capable of inducing primary CD8+ CTL. (duke.edu)
We employed in vitro cultures and two in vivo models to investigate whether androgens exert these effects directly on marrow lymphoid precursors or whether actions on marrow stromal elements are required. (jci.org)

DNA damage in lymphocytes, as measured by alkaline single cell gel electrophoresis (pH 12.7), is greatly increased by the concurrent lysis of whole blood in both freshly isolated samples and in PHA-stimulated cultures over a period of 7 days. (elsevierpure.com)
Further, the progressive increase in DNA damage in whole blood cultures may influence other methods using lymphocytes for population biomonitoring and may be significant for in vitro genotoxicity testing. (elsevierpure.com)

SFTG international collaborative study on in vitro micronucleus test II. (maastrichtuniversity.nl)

The Dog T Lymphocyte Cocktail is a three-color reagent cocktail designed to identify dog T lymphocytes by direct immunofluorescence staining with flow cytometric analysis. (bdbiosciences.com)

Transfection of mutant CD3ε chains lacking these residues inhibited the differentiation of mature CD4 + Foxp3- T lymphocytes into CD4 + Foxp3 + Tregs, and differences in CD3ε chain recognition by antibodies could be used to enrich for Tregs in vivo. (urosario.edu.co)

MART-1 27-35 -specific CTL could be effectively generated from a total of 11 of 12 PBL and from 3 of 3 TIL derived from HLA-A2 + melanoma patients, as well as from 2 of 4 PBL from HLA-A2 + healthy donors by in vitro stimulation with autologous PBMC pulsed with the synthetic MART-1 27-35 peptide. (elsevierpure.com)

This clone will facilitate investigations into the role of viral genes on biological properties of HTLV-1 in vitro and in vivo. (wustl.edu)

Temporary increase in granular lymphocytes was associated with survival of patients with severe infection. (bvsalud.org)

Lymphocyte studies were not performed on the other 2 patients. (cdc.gov)
In vitro studies of the metabolism of atrazine, simazine, and terbutryn in several vertebrate species. (cdc.gov)
Based on the results of the three in vitro studies, the test substance is considered to have no genotoxicity potential. (europa.eu)
Two in vitro studies are available. (europa.eu)

The effect of PSK, a protein-bound polysaccharide and an immunomodulator, on lymphocytes was examined in vitro for 36 patients with gastric cancer and 26 with colorectal cancer. (unboundmedicine.com)

Lymphocytes were cultured for 48 ± 2 h and thereafter exposed in duplicate to selected doses of test substance for 3 h in the absence and presence of S9-mix. (europa.eu)

Oligoclonal expansion of T lymphocytes with multiple second-site mutations leads to Omenn syndrome in a patient with RAG1-deficient severe combined immunodeficiency. (lu.se)
V(D)J recombination defects in lymphocytes due to RAG mutations: severe immunodeficiency with a spectrum of clinical presentations. (lu.se)

Anatomy46

Organisms14

Diseases16

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes50

Disciplines and Occupations1

Information Science3

Health Care1

Peripheral11

Proliferation9

Reactivity3

Assay1

Vivo and in vitro1

Human12

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Antigens1

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Cells19

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CD251

Alloantigens1

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Investigate2

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