Vitro hep g2 cells. Medical search. Frequent questions

These in vitro data are relevant with those found in vivo in insulin-resistant patients. (jci.org)
With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
In vivo antitumor activity and toxicity were evaluated in Hep G2 cell xenograft model. (dovepress.com)

Another well-characterized polarized hepatocyte cell line is the rat hepatoma-derived hybrid cell line WIF-B. With the proper culture conditions, Hep G2 cells display robust morphological and functional differentiation with a controllable formation of apical and basolateral cell surface domains (van IJzendoorn et al. (wikipedia.org)
Plasminogen activator inhibitor-1 synthesis in the human hepatoma cell line Hep G2. (jci.org)
The hepatoma cell line Hep G2 has been shown to synthesize PAI-1 in response to insulin. (jci.org)
In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
Cytotoxicity, antiproliferative and apoptosis-inducing capacity of ACNVs against hepatoma carcinoma cell were assessed. (dovepress.com)

Because of their high degree of morphological and functional differentiation in vitro, Hep G2 cells are a suitable model to study the intracellular trafficking and dynamics of bile canalicular, sinusoidal membrane proteins, and lipids in human hepatocytes in vitro. (wikipedia.org)
Hep G2 cells may be a suitable model to study PAI-1 regulation in response to hyperinsulinemia. (jci.org)

NC exhibited rapid cellular uptake and stronger apoptotic effect (~46.6% reduced IC 50 value) than FC, blocking HepG2 cells at G2/M phase. (bgu.ac.il)
The results indicate that PAI-1 synthesis in presence of insulin is markedly increased in down-regulated cells, and that metformin inhibits this effect by acting at the cellular level. (jci.org)
With further technological developments, these human in vitro cellular models shall be highly useful in the screening of compounds for personalized medicine, allowing optimum therapy with minimum or no adverse effects, and in the study of adverse outcomes in different strata of population. (brill.com)
In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
Liver hepatocellular carcinoma cell line HepG2 was used as a model to study induced liver inflammation in vitro at the cellular, genetic, and epigenetic levels. (illinois.edu)
Cellular apoptosis (ie, programmed cell death) is another potential mechanism of many antineoplastic agents. (medscape.com)

Hypomethylation may restore normal function to genes critical for cell differentiation and proliferation. (medscape.com)

In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. (brill.com)
Systems toxicology, therefore, aims at understanding and exploring the way that different biological components are orchestrated as an ensemble in cells, tissues, and organisms. (brill.com)
Toxicology in Vitro , 65 , Article 104797. (illinois.edu)
Wen, Y, Mirji, N & Irudayaraj, J 2020, ' Epigenetic toxicity of PFOA and GenX in HepG2 cells and their role in lipid metabolism ', Toxicology in Vitro , vol. 65, 104797. (illinois.edu)

Furthermore, treatment of s.c xenografts derived from A549 cells with intratumor injections of terpinen-4-ol significantly inhibited tumor growth compared with the control group. (hindawi.com)

The aim of this study was to analyze the insulin-mediated response of PAI-1 and lipid synthesis in Hep G2 cells after producing an insulin-resistant state by decreasing insulin receptor numbers. (jci.org)
mRNA expression analysis of PFOA exposed cells showed that cell cycle homeostasis genes were affected significantly, as well as the ten-eleven translocation methylcytosine dioxygenases (TETs) and the essential lipid metabolism genes. (illinois.edu)

Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)
Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)

First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
ACNVs were found to possess specific antitumor cell proliferation activity associated with an apoptosis-inducing pathway. (dovepress.com)
It inhibits cell proliferation by inhibiting DNA and RNA polymerase. (medscape.com)
It inhibits topoisomerase II and causes DNA strand breakage, which arrests cell proliferation in the late S or early G2 portion of cell cycle. (medscape.com)

citation needed] Hep G2 cells are a suitable in vitro model system for the study of polarized human hepatocytes. (wikipedia.org)

citation needed] Hep G2 cells and their derivatives are also used as a model system for studies of liver metabolism and toxicity of xenobiotics, the detection of environmental and dietary cytotoxic and genotoxic (and thus cytoprotective, anti-genotoxic, and cogenotoxic) agents, understanding hepatocarcinogenesis[citation needed], and for drug targeting studies[citation needed]. (wikipedia.org)

A long-acting fluorinated corticosteroid, dexamethasone induces apoptosis of leukemia cells by means of glucocorticoid receptors. (medscape.com)

Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. (wikipedia.org)

Extracellular vesicles (EVs) are a group of cell-derived membranous structures, which play an important role in intercellular communication and functional transduction. (dovepress.com)

Preincubation by an 18-h exposure of Hep G2 cells to 10(-7) M insulin aimed at reducing the number of insulin receptors, was followed by a subsequent 24-h stimulation with 10(-9) M insulin. (jci.org)
Global methylation levels of HepG2 cells were found to be inversely proportional to PFOA exposure levels. (illinois.edu)

ACNVs could be internalized into tumor cells mainly via phagocytosis, but they were quickly cleared once entering the blood. (dovepress.com)
Furthermore, the high refractoriness of liver cancer to treatments has been associated with the interaction of very complex and diverse mechanisms of chemoresistance, which can act synergistically to protect tumor cells from the chemotherapy agents [ 3 ] . (encyclopedia.pub)

Komor, Badran and Liu, 2017 ) have opened up tremendous opportunities for the development of cell lines, especially those of human origin ( Tobita, Guzman-Lepe and de L'Hortet, 2015 ). (brill.com)
Since 2015, more than 3,000 articles were published on studies using crispr /Cas9 genome editing, including more than 900 articles using the technology in mammalian cells (PubMed, accessed June 11, 2017). (brill.com)

Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. (hindawi.com)
Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)

This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. (blogspot.com)
Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. (blogspot.com)

1 , 2 In recent years, mammalian cell-derived exosomes (a type of extracellular vesicle) have been widely used in the field of biomedicine, including diagnosis, regulation of disease progression and drug delivery. (dovepress.com)

Hep G2 (or HepG2) is a human liver cancer cell line. (wikipedia.org)
Hep G2 will respond to stimulation with human growth hormone. (wikipedia.org)
This can be important for the study of human liver diseases that are caused by an incorrect subcellular distribution of cell surface proteins, e.g., hepatocanalicular transport defects such as Dubin-Johnson Syndrome and progressive familial intrahepatic cholestasis (PFIC), and familial hypercholesterolemia. (wikipedia.org)
In addition, there is no report on the antitumor effects of terpinen-4-ol against human nonsmall cell lung cancer cells. (hindawi.com)
Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)
The A549 human lung adenocarcinoma and CL1-0 lung adenocarcinoma cell lines were cultured in Dulbecco′s modified eagle medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic antimycotic. (hindawi.com)
More recently, additional human and animal cell lines that support SARS-CoV replication have been identified ( 15 ). (cdc.gov)

The cells secrete a variety of major plasma proteins, e.g., albumin, and the acute-phase proteins fibrinogen, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. (wikipedia.org)
3 , 4 Similarly, plant cells also secrete exosome-like nanovesicles (ELNVs) that can participate in various physiological processes such as the inheritance of traits and defense response. (dovepress.com)

Hep G2 cells are also employed in trials with bio-artificial liver devices[citation needed]. (wikipedia.org)
These drawbacks may be explained, at least in part, by the various phenotypes and histological characteristics of tumor liver cells and its microenvironment [ 5 ] . (encyclopedia.pub)

CONCLUSION: QJD significantly increased body weight, decreased water content in feces, relieved inflammatory cell infiltration, maintained the structure of duodenum and colon. (bvsalud.org)

The endocytosis mechanism of ACNVs was evaluated on Hep G2 cells in the presence of different endocytosis inhibitors. (dovepress.com)

Exosomes are a type of membrane vesicles secreted by living cells. (dovepress.com)

Cancer chemotherapy is based on an understanding of tumor cell growth and of how drugs affect this growth. (medscape.com)
This difference allows normal cells to recover from chemotherapy more quickly than malignant cells, and it is in part the rationale for current cyclic dosage schedules. (medscape.com)

Hepatitis B virus surface antigens have not been detected. (wikipedia.org)
The major risk factors include chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, environmental carcinogens such as aflatoxin B1 (AFB1), alcoholic cirrhosis, and inherited genetic disorder such as hemochromatosis, Wilson's disease, and tyrosinemia. (hindawi.com)
Given the potential for laboratory-associated severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infections, we must know which cell lines are susceptible to the virus. (cdc.gov)
We investigated 21 cell lines routinely used for virus isolation or research. (cdc.gov)
Simultaneously, these investigations showed that SARS-CoV could not replicate in a number of other cell lines routinely used for respiratory virus isolation. (cdc.gov)
Because SARS-CoV is classified as a risk group level 4 pathogen in Australia, all procedures performed with the virus, including infecting cell lines and viral lysis before RNA extraction, were carried out in a physical containment level 4 (PC4) laboratory. (cdc.gov)

After infection with SARS-CoV, cells were observed for cytopathic effects, and quantitative real-time polymerase chain reaction was used to measure ongoing viral replication. (cdc.gov)
Given the potential for SARS-CoV infection to occur in a laboratory setting, we must be aware of cell lines in which it can replicate. (cdc.gov)

Both POE and PJ effectively reduced Disease Activity Index scores and inflammatory cell infiltration in the UC mouse model, but PJ had a better effect than POE. (bvsalud.org)

The study identified 10 new cell lines capable of supporting the replication of SARS-CoV and confirmed the susceptibility of 4 cell lines previously reported. (cdc.gov)
This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes. (cdc.gov)

These cells were derived from a variety of species and tissues and included those capable of supporting the replication of respiratory and enteric viruses. (cdc.gov)

In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)

The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)

These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. (wikipedia.org)

The Journal of Cell Biology. (wikipedia.org)

Among lung cancers, nonsmall cell lung carcinomas (NSCLC) account for approximately 80% of lung cancer cases [ 1 ]. (hindawi.com)

Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)

Flowcytometry was performed to evaluate the cell cycle analysis of corneal keratocytes. (blogspot.com)

SARS-CoV was first isolated in Vero E6 and FRhK cells injected with clinical specimens as part of early attempts to identify the etiologic agent of SARS ( 10 , 14 ). (cdc.gov)
This review describes current considerations in the use of hydroxyurea for the management of sickle cell disease in the context of clinical severity. (cdc.gov)

It also shows that SARS-CoV can achieve high titers in several cell lines, sometimes in the absence of specific cytopathic effects. (cdc.gov)
An isolate of SARS-CoV, strain HKU 39849, was passaged on 2 occasions in Vero E6 cells to establish a high-titer stock that was used in all infectivity experiments. (cdc.gov)
Rather, its genotoxic effects are limited to indirect clastogenicity occurring in select cell types, and only when high dose and time thresholds are exceeded. (cdc.gov)

After cells divide, they enter a period of growth (G1 phase), followed by DNA synthesis (S phase). (medscape.com)
The next phase is a premitotic phase (G2 phase). (medscape.com)
Finally, a phase of mitotic cell division (M phase) occurs. (medscape.com)

Notably, terpinen-4-ol was able to increase p53 levels in A549 and CL1-0 cells. (hindawi.com)

Anatomy10

Organisms1

Diseases4

Chemicals and Drugs72

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes24

Information Science3

Vivo3

Hepatoma5

Suitable model to study2

Cellular6

Differentiation1

Toxicology4

Inhibited tumor1

Lipid2

Apoptotic2

Proliferation5

Hepatocytes1

Toxicity1

Induces Apoptosis1

Hepatocellular carcinoma1

Functional1

Exposure2

Tumor cells2

20172

Apoptosis6

Gene2

Mammalian1

Human7

Secrete2

Liver2

Significantly1

Mechanism1

Membrane1

Chemotherapy2

Virus6

Infection2

Model1

Study2

Replication1

Cancer cell1

Expression1

Morphology1

Biology1

Cancers1

Studies1

Cycle1

Clinical2

High3

Phase3

Levels1