Vitro epithelial cells. Medical search. Frequent questions

High-efficiency neoplastic transformation of mouse mammary epithelial cells in primary collagen gel culture was induced by N-methyl-N-nitrosourea (MNU). (eurekamag.com)
Mammary epithelial cells, isolated from virgin BALB/c mice, were embedded within collagen gels and grown in a serum-free medium containing prolactin, progesterone, and linoleic acid. (eurekamag.com)
Eleven to 14 days after the final carcinogen treatment, the cells were removed from the collagen gels and injected into the cleared mammary fat pads of syngeneic hosts to assay for transformed cell populations. (eurekamag.com)
Control cells cultured for the same periods of time as MNU-treated cells produced only ductal outgrowths that were morphologically similar to those found in the mammary glands of adult virgin hosts. (eurekamag.com)
Background: Bovine mammary epithelial cells after calving undergo serious metabolic challenges and oxidative stress both of which could compromise autophagy. (illinois.edu)
However, effects of TFEB-mediated autophagy on the oxidative stress of bovine mammary epithelial cells remain unknown. (illinois.edu)
Therefore, the main aim of the study was to investigate the role of TFEB-mediated autophagy in bovine mammary epithelial cells experiencing oxidative stress. (illinois.edu)
Results: H 2 O 2 challenge of the bovine mammary epithelial cell MAC-T increased protein abundance of LC3-II, increased number of autophagosomes and autolysosomes while decreased protein abundance of p62. (illinois.edu)
To investigate the basis of this differential susceptibility, three cell lines were developed from the oral epithelium (BBR/OE), mammary epithelium (BBR/ME), and stromal mammary fibroblasts (BBR/MFib) of transgenc Big Blue rats. (uoguelph.ca)
When the cells were treated with N-OH-PbIP, the epithelial cells were again more sensitive to cytotoxicity but a small increase in mutagenicity was observed for both mammary cell lines. (uoguelph.ca)
In contrast, greater 'O'-acetyltransferase expression was measured in the mammary epithelial cells. (uoguelph.ca)
Adipocyte-Derived Paracrine Factors Regulate the In Vitro Development of Bovine Mammary Epithelial Cells. (bvsalud.org)
The mammary gland is composed of epithelial tissue forming ducts and lobules, and the stroma, composed of adipocytes , connective tissue , and other cell types. (bvsalud.org)
The stromal microenvironment regulates mammary gland development by paracrine and cell - cell interactions . (bvsalud.org)
In the present study, primary cultures of bovine mammary epithelial cells (bMEC) and bovine adipose-derived stem cells (bASC) subjected to adipogenic differentiation were used to investigate the influence of paracrine factors secreted by preadipocytes and adipocytes on bMEC development. (bvsalud.org)
The bovine mammary epithelial cell undergoes serious metabolic challenges and excessive ROS production during the transition period. (biomedcentral.com)
Because apoptosis of bovine mammary epithelial cells is modulated by cellular redox state [ 11 ], developing potential strategies for maintaining redox balance is particularly important in terms of preventing oxidative damage. (biomedcentral.com)
Thus, TFEB-mediated autophagy in bovine mammary epithelial cells might play a positive role in response to oxidative stress. (biomedcentral.com)
Ectopic expression of AUF1 also promoted stemness in mammary epithelial cells, and thereby increased the proportion of cancer stem cells. (nature.com)
These data prompted us to ask whether AUF1 upregulation could promote EMT as well as stemness in mammary epithelial cells. (nature.com)
Here, we have investigated the role of ChoK in the development of breast cancer and found that ChoK is both necessary and sufficient for growth factor-induced proliferation in primary human mammary epithelial cells and an absolute requirement for the specific mitogenic response to heregulin in breast tumor-derived cells. (aacrjournals.org)
These results demonstrate that ChoK plays an essential role in both normal human mammary epithelial cell proliferation and breast tumor progression. (aacrjournals.org)

In this study, the human bronchial epithelial cells (16HBE) were treated five times with 1 ?M benzo(a)pyrene (BaP), followed by 2 passages culture, and thein vitroBaP-induced malignant transformation of 16HBE cells was established. (eurekamag.com)
Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)
In this study, we utilized primary normal human bronchial epithelial (NHBE) cells that are cultured at an air-liquid interface (ALI) to mimic the in vivo airway characteristics 30 . (nature.com)
Using a physiologically relevant air-liquid interface exposure system, electrically tight monolayers of the human bronchial epithelial cell-line Calu-3 were exposed to fresh, whole mainstream cigarette smoke. (nih.gov)
This pulmonary exposure to alcohol can damage the bronchial epithelial layer of the lung [ 6 , 7 ], while impairing the lung's ability to repair epithelial wounding via inhibition of the PKA pathway [ 8 ]. (cdc.gov)

In summary, co-culture with TPSCs induced molecular changes associated with EMT in pancreatic cancer cells, regardless of differentiation status, and the organotypic model demonstrated the influence of microenvironmental factors, such as glucose, stellate cells and macrophages, on pancreatic cancer cell invasion. (lu.se)
Physiological shear stress enhances differentiation and mucus-formation of intestinal epithelial cells in vitro. (authorea.com)
Differentiation of a confluent monolayer of human mucus-producing epithelial HT29-MTX cells was monitored under dynamic and static culture conditions. (authorea.com)
We demonstrated the impact of culture conditions on the differentiation of HT29-MTX cells, revealing outstanding in vivo like reorganization of cells and the production of thick adherent mucus networks when cultured under physiological shear stress using our newly designed culture chamber. (authorea.com)
The 3dGRO™ Human Lung Organoid Culture System is a serum-free, multi-stage culture system for efficient differentiation of human induced pluripotent stem (iPS) cells to mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. (sigmaaldrich.com)
The inverse correlation between Notch signaling and the proliferative status of the corneal epithelium is consistent with the idea that Notch plays a role in corneal epithelial differentiation. (molvis.org)
MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)

Effect of modifying quantum dot surface charge on airway epithelial cell uptake in vitro. (cdc.gov)
Here, we report on the influence of nanoparticle charge and dose on human airway epithelial cells, and show that this platform can be used to evaluate consequences of exposure to engineered nanomaterial s. (cdc.gov)
Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)
Because alcohol blocks airway epithelial cell release of IL-6 in vitro , we hypothesized that alcohol exposure would alter mouse lung inflammatory responses to HDE. (cdc.gov)

In this study, we investigated the antioxidant effect of monoammonium glycyrrhizinate (MAG) on calf intestinal epithelial cells (CIECs) exposed to heat stress in vitro. (asas.org)
In vitro characterization revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells. (frontiersin.org)

Alveolar epithelial cells (AEC) have been implicated in pathological remodelling. (lu.se)
Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)

In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (nih.gov)
DESIGN: Proliferation, mitosis and the expression of progenitor lineage markers were assessed in normal squamous oesophagus from 10 patients by immunofluorescence on 3D epithelial whole mounts. (who.int)

Inhibition of autophagy via bafilomycin A1 aggravated H 2 O 2 -induced reactive oxygen species (ROS) accumulation and apoptosis in MAC-T cells. (illinois.edu)
Next, bMEC were cultured for 24 h in CM and cell viability , apoptosis , migratory activity, ability to form spheroids on Matrigel, and secretory activity (alpha S1- casein concentration) were evaluated. (bvsalud.org)
Cell viability was estimated by cell counting kit-8 and cell apoptosis by flow cytometry. (karger.com)
In LPS-induced ARDS cell model, astragaloside IV up-regulated cell viability, SOD activity and ZO-1 and LC3B I expressions but down-regulated cell apoptosis, TNF-α, IL-6, LC3B II, Beclin-1 and atg5 expressions and LDH and MDA levels. (karger.com)
Astragaloside IV might suppress autophagy initiation directly or indirectly through suppressing the oxidative stress and inflammatory response, which further enhances the cell viability and tight junction and reduces apoptosis in LPS-stimulated pulmonary endothelial ARDS cell model, thus exerting its therapeutic function in ARDS. (karger.com)
Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
Conclusions and Clinical Relevance - T foetus and its CP30 induce apoptosis in cultured BUECs in vitro. (avma.org)

Objective -To determine the effects of 2 conjugated linoleic acid (CLA) isomers (cis-9, trans-11 and trans-10, cis-12) on synthesis of prostaglandin (PG) E 2 and F 2α and expression of prostaglandin H synthase-2 (PGHS-2) of adult and fetal bovine endometrial epithelial cells in vitro. (avma.org)
Sample -Primary cultures of endometrial epithelial cells obtained from 4 adult cows and 4 fetal bovine carcasses. (avma.org)
Objective -To evaluate the cytopathic effects of Tritrichomonas foetus and a purified cysteine protease (ie, CP30) of T foetus on cultured bovine uterine epithelial cells (BUECs) in vitro. (avma.org)

After co-culture with tumour-associated pancreatic stellate cells (TPSCs), pancreatic cancer cells displayed up to 8-fold reduction in levels of epithelial-mesenchymal transition (EMT) markers E-cadherin and ZO-1, while β-catenin and vimentin levels were increased. (lu.se)
Epithelial-mesenchymal transformation is now recognised as an important feature of tissue remodelling. (ersjournals.com)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
In the present study, the hypothesis that adenoviral infection followed by persistent expression of its E1A gene is capable of producing epithelial-mesenchymal transformation was examined by investigating guinea pig PLECs that express adenoviral E1A. (ersjournals.com)
Since epithelial-to-mesenchymal transition (EMT) and the acquisition of cancer stem cell traits are important for cancer onset and progression, we sought to determine the role of AUF1 in these two important processes. (nature.com)
During prenatal development and postnatal cyclic hair regeneration, hair follicle morphogenesis is triggered by reciprocal interactions and the organization of the epithelial and mesenchymal cell po. (researchgate.net)
Sarcomatoid conversion of clear cell renal cell carcinoma in relation to epithelial-to-mesenchymal transition. (lu.se)
Approximately 8% of clear cell renal cell carcinoma cases contain regions of radically different morphology, demonstrating a mesenchymal appearance histologically resembling sarcomas. (lu.se)
In the present study we investigate whether the sarcomatoid conversion of clear cell renal cell carcinoma could be interpreted as linked to the process of epithelial-mesenchymal transition. (lu.se)
Furthermore the sarcomatoid elements demonstrate an enhanced expression of epithelial-mesenchymal transition related mesenchymal markers as compared with the clear cell renal cell carcinoma counterparts. (lu.se)
We further selected a representative case, clinically demonstrating direct overgrowth of the sarcomatoid component into the liver and colon, for extended immunohistochemical characterization, resulting in a further set of positive and negative epithelial-mesenchymal transition markers as well as pronounced transforming growth factor β positivity, indicating that sarcomatoid clear cell renal cell carcinoma may be associated to epithelial-mesenchymal transition. (lu.se)
Transforming growth factor β1 exposure of in vitro cultured primary clear cell renal cell carcinoma cells resulted in cells adopting a mesenchymal morphology similar to sarcomatoid clear cell renal cell carcinoma. (lu.se)
Corresponding changes in RNA levels for key epithelial-mesenchymal transition markers were also seen. (lu.se)
We therefore suggest that sarcomatoid clear cell renal cell carcinoma morphologically and immunohistochemically may represent a completed epithelial-mesenchymal transition and that transforming growth factor β1 could be an important driving force during the sarcomatoid transdifferentiation of clear cell renal cell carcinoma. (lu.se)

This exposure results in a regulated, dose-dependent loss of epithelial barrier function in the lung epithelial monolayers. (nih.gov)
Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)

AFE cells may be cryopreserved (SCC301) or further differentiated into branching lung bud organoids using 3dGRO™ Lung Organoid Branching Medium (SCM307) and further matured into branching and alveolar lung organoids using 3dGRO™ Lung Organoid Maturation Medium (SCM308). (sigmaaldrich.com)

Analyze cells by flow cytometry on day 4. (sigmaaldrich.com)
Cells expressing differential levels of epithelial and progenitor markers were isolated using flow cytometry sorting and characterised by qPCR and IF. (who.int)

To demonstrate differences in human lens epithelial cell adhesion to different intraocular lens biomaterials in vitro and to determine whether these differences can be influenced by coating the intraocular lens surface with commercially available fibronectin. (arvojournals.org)
Lung organoids are useful three-dimensional (3D) cell culture models for studying human lung development and respiratory diseases including viral infections (SARS-CoV, H1N1, MERS), cystic fibrosis, asthma/COPD, exposure to air pollution and the effects of smoking. (sigmaaldrich.com)
Human pluripotent stem cells are differentiated into definitive endoderm cells using a 4-day induction medium. (sigmaaldrich.com)
During days 4-8, induction media (SCM305, SCM306) directs human definitive endoderm cells toward anterior foregut endoderm (AFE). (sigmaaldrich.com)
Add ROCK inhibitor (ROCKi) Y-27632 ( SCM075 ) to 7-10 mL Human ES/iPS cell Expansion Media ( SCM130 ) to a final concentration of 10 µM. (sigmaaldrich.com)
entericaLT21 on human laryngeal carcinoma cell line (HEp2). (md-medicaldata.com)
Human epithelial cells in vitro - Are they an advantageous tool to help understand the nanomaterial-biological barrier interaction? (unifr.ch)
The aim of this study was to analyze the in vitro antimicrobial efficacy and the in vitro effects of an ophthalmic formulation containing hexamidine diisethionate 0.05%, polyhexamethylene biguanide (PHMB) 0.0001% disodium edetate (EDTA) 0.01%, dexpanthenol 5% and polyvinyl alcohol 1.25% (Keratosept, Bruschettini, Genova, Italy) on cultured human corneal and conjunctival cells. (unifi.it)
Human corneal and conjunctival epithelial cells in vitro were incubated for 5, 10 and 15 min with Keratosept or its components. (unifi.it)
Our results demonstrate that the smoke-induced loss of epithelial barrier function in human bronchial epithelium is a regulated process rather than a cytotoxic response. (nih.gov)
Overall primary concerns are: extrapolating human clinical studies, seeding durable effective T cell resident memory (Trm), population human leucocyte antigen (HLA) coverage, and the potential for T cell-mediated immune escape. (mdpi.com)
Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. (jax.org)
Human recombinant interleukin-2 activated sheep lymphocytes lyse sheep pulmonary microvascular endothelial cells. (hopkinsmedicine.org)
In vitro models of human hair follicle-like tissue could be fundamental tools to better understand hair follicle morphogenesis and hair drug screening. (researchgate.net)
Results showed that in cultured human-derived retinal pigment epithelial ARPE-19 cells , the extract of Chlorella zofingiensis and its nutritional ingredient astaxanthin exhibited significant inhibitory effects on the formation of endogenous N ε -carboxymethyllysine (CML), a key AGE representative, through the suppression of intracellular oxidative stress. (rsc.org)
Another clear ambition of Martin's research is to create, study and promote alternative in vitro models that mimic in vivo environments, in order to gain a mechanistic understanding of how nanoparticles may, potentially, adversely impact human health. (swansea.ac.uk)
Furthermore, by creating sophisticated in vitro multi-cellular models of different human organs in combination with state-of-the art microscopic and biochemical analyses, Martin is able to obtain a mechanistic understanding of the health risks posed by nanomaterials under realistic conditions, as well as gain valuable insight into the potential of in vitro as alternative test systems to in vivo methodologies. (swansea.ac.uk)
Atypical patterns of human cytomegalovirus infection and spread in U373 glioblastoma cells. (uc.edu)
Rescue of Pentamer-Null Strains of Human Cytomegalovirus in Epithelial Cells by Use of Histone Deacetylase Inhibitors Reveals an Additional Postentry Function for the Pentamer Complex. (uc.edu)
Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers. (uc.edu)
Development of a Primary Human Cell Model for the Study of Human Cytomegalovirus Replication and Spread within Salivary Epithelium. (uc.edu)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)

Unlike traditional immortalized pulmonary cell lines and primary cells, lung organoids contain various differentiated cell types with complex tissue architecture that more closely resemble in vivo tissues and functionality. (sigmaaldrich.com)
In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (nih.gov)
She serves on NIH study sections and as reviewer for journals in her area of expertise, manages an active laboratory, supervises students in pulmonary biology and epithelial cell research and has received awards recognizing both teaching and original research accomplishments. (rochester.edu)

It also induces conversion of RPE cells toward a fibroblastlike phenotype. (medscape.com)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin-5 (AQP5). (hopkinsmedicine.org)

For the direct-acting mutagen 'N'-ethyl-' N'-nitrosourea, all cell lines were equally sensitive to mutagenesis in the 'cII' gene but the fibroblast cell line showed greater resistance to cytotoxicity. (uoguelph.ca)

RT-PCR analysis showed that cytochrome P450 enzymes 1A1 and 1B1 are expressed at low levels in both cell lines, with greater 1B1 expression seen in the fibroblast cells. (uoguelph.ca)
Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. (nature.com)
Proteomic studies were also performed using tandem mass spectrometry analysis (MS/MS). Results imply that S. entericaLT21 affects the expression of proteins of HEp2 cells and moreover HEp2 cells after incubation with S. entericaLT21 additionally expressed protein troponin T type 1. (md-medicaldata.com)
We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (nih.gov)
These two cell types differed, however, in their NF-κB and AP-1 binding after lipopolysaccharide stimulation, possibly due to differences in the expression of the subunits that comprise these transcriptional complexes. (ersjournals.com)
The expression of Hes1 was evaluated in cell culture. (molvis.org)
Expression of Notch1 , Jagged1 , and Hes1 was found predominantly in the basal and immediate suprabasal cells. (molvis.org)
In vitro, the expression of Hes1 was higher in confluent cells maintained under high calcium conditions. (molvis.org)
Expression of Aquaporin-1 water channel protein in mouse erythro-leukemia cells: genetic regulation of corticosteroid and chemical induction. (hopkinsmedicine.org)
LPS effectively inhibited cell viability and LC3B I expression and enhanced LC3B II, Beclin-1 and atg5 expressions in MLE-12 cells. (karger.com)
3-methyladenine promoted cell viability and ZO-1 expression, down-regulated Beclin-1 and atg5 expression, while Rapamycin (Rap) had an opposite effect. (karger.com)
Astragaloside IV suppressed cell viability and ZO-1 expression after the Rap treatment. (karger.com)
Thus, we conclude that As2O3 can reduce metastasis potential of NPC cells, involving inhibition of MMP-9 expression. (scielo.br)
Exposure of cells to CLAs did not affect expression of PGHS-2 protein. (avma.org)
PGF 2α concentration ratios for cultures of adult and fetal endometrial epithelial cells with no apparent effect on PGHS-2 expression. (avma.org)

S-nitrosylation of β-catenin by eNOS-derived NO promotes VEGF-induced endothelial cell permeability. (md-medicaldata.com)
OVDs can create and preserve surgical space, pressurize the anterior chamber in the presence of an open wound, partition spaces, protect the corneal endothelial cells from fluid turbulence, and facilitate surgery. (lww.com)

A single exposure or multiple exposures of these cells to MNU was effective in inducing tumorigenic cells that produced palpable tumors as early as 6 weeks after transplantation. (eurekamag.com)
A few of these inflammatory responses, such as inflammatory cell recruitment, can be modulated by alcohol exposure [ 1 , 2 , 3 , 4 ]. (cdc.gov)

The advantages and disadvantages of the different in vitro models are discussed in order to provide a clear overview as to whether or not epithelial cell cultures are an advantageous model to be used for basic mechanism and nanotoxicology research. (unifr.ch)

Importantly, breast cancer cells that ectopically express AUF1 were more efficient in forming orthotopic tumor xenografts in nude mice than their corresponding controls with limiting cell inocula. (nature.com)
Using 6 biphasic clear cell renal cell carcinoma cases we show that sarcomatoid clear cell renal cell carcinoma shares characteristic markers associated with loss of von Hippel-Lindau tumor suppressor with conventional clear cell renal cell carcinoma and also exhibits a markedly higher proliferative index. (lu.se)

The protein content in HEp2 cells after the incubation with S. entericaLT21 was investigated and compared with the protein content in the untreated HEp2 cells. (md-medicaldata.com)
Upon nutrient deprivation, autophagy is often up-regulated in response to metabolic stress and excessive organelle damage to facilitate aggregated protein removal and provide energy for cells [ 13 ]. (biomedcentral.com)
HeLa cells expressing Myc-GnT1IP-L (Myc-tagged GlcNAcT-1 inhibitory protein, long transcript, membrane-anchored) (red) and GlcNAcT-1-HA (green) were treated with cycloheximide and brefeldin A to cause retrograde transport of Golgi enzymes into the ER. (cellimagelibrary.org)
In the most well characterized "canonical" pathway in mammalian cells, the Notch intracellular domain (NotchIC) translocates to the nucleus where it mostly associates with the recombination signal binding protein for the immunoglobulin kappa J region (RBPJκ). (molvis.org)
Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and stained for acetylated tubulin of the primary cilium (red) by immunofluorescence. (ucsd.edu)
See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details. (ucsd.edu)

Mutagenicity was observed in the fibroblast cells, but the greater toxicity of PhIP to the epithelial cells prevented a definitive evaluation of mutagenicity. (uoguelph.ca)

The Journal of Cell Biology. (wikipedia.org)
Results have been partially published in Frontiers in Cell and Developmental Biology and in Breast Cancer research, being myself as a senior author. (jove.com)

Additionally, lung organoids can be derived from small amounts of patient tissues or pluripotent stem cells to create living biobanks that facilitate personalized biomedical research. (sigmaaldrich.com)
Such continuously renewing epithelia are by definition governed by stem cells. (molvis.org)
Upon division, the slow cycling limbal epithelial stem cells give rise to daughter cells known as transient amplifying (TA) cells. (molvis.org)
Together, these findings provide clear evidence that AUF1 is an important inducer of the EMT process through stabilization of SNAIL1 and TWIST1 and the consequent promotion of breast cancer stem cells. (nature.com)
Hair regeneration using hair follicle stem cells (HFSCs) and dermal papilla cells is a promising approach for the treatment of alopecia. (researchgate.net)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)

The apical-most epithelial intercellular junction, i.e., the tight junction, regulates paracellular solute and ion permeability. (jove.com)

Our results demonstrated that BaP could induce the malignant transformation of 16HBE cells, and p53 and p-Akt (Ser473) might play crucial roles in BaP-induced carcinogenesis. (eurekamag.com)

The ability of the adhesion of S. entericaLT21 as an independent culture and in combination with L. plantarum16.4 to HEp2 cells was tested as well as metabolic activity of S. entericaLT21 after adhesion to HEp2 cells. (md-medicaldata.com)
the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to evaluate the metabolic cell activity. (unifi.it)

Tight junctions between adjacent epithelial cells constitute the physiological barrier to fluid and macromolecules in epithelium. (nih.gov)
The corneal epithelium is continually losing cells due to desquamation (primarily via the blink reflex) and thus must replace these cells to maintain homeostasis. (molvis.org)

1997. Induction of apoptotic cell death by particulate lead chromate: Differential effects of vitamins C and E on genotoxicity and survival. (cdc.gov)

John Philip "Trink" Trinkaus (23 May 1918, Rockville Centre, New York - 8 February 2003, Guilford, Connecticut) was an American embryologist and one of the world's leading experts on in vivo cell motility. (wikipedia.org)
In order to initiate the reduction, refinement and replacement of time consuming, expensive and stressful (to the animals) in vivo experimental approaches, many in vitro epithelial cell culture models have been developed during the last decades. (unifr.ch)

IPF mucus cells expressed MUC5B but low levels of MUC5AC and MUC2, a profile typical of submucosal glands. (bmj.com)

A 3D organotypic model showed that TPSCs stimulated pancreatic cancer cell. (lu.se)
A 3D organotypic model showed that TPSCs stimulated pancreatic cancer cell invasion, both as single cell (PANC-1) and cohort (MIAPaCa-2) invasion. (lu.se)
Their self-renewing potential was investigated by colony forming cells assays and in vitro organotypic culture models. (who.int)

100 publications within the field of nanotoxicology and the nanoparticle-cell interaction in vitro . (swansea.ac.uk)

High glucose conditions further enhanced changes in EMT markers as well as the cancer cell invasion. (lu.se)

Histopathological changes in the tumors indicated nests growth, high nuclear-cytoplasmic ratios, coarse and clumped chromatin, numerous and distinctly atypical mitoses, cell necrosis and surrounding normal adipose, muscle and connective tissue immersed. (eurekamag.com)
The Forces That Shape the Embryo became a classic treatise on cell and tissue movement during embryonic development and cell invasion of bodily tissues. (wikipedia.org)
VEGF upregulates the profibrotic growth factor connective tissue growth factor (CTGF) in various cell types in the newly formed neovascular membranes. (medscape.com)
2022) Targeting p21Cip1 highly expressing cells in adipose tissue alleviates insulin resistance in obesity. (jax.org)

Once in the vitreous cavity, the RPE cells undergo morphologic changes where they attain fibroblastlike activity, secreting growth factors that stimulate collagen and fibronectin production. (medscape.com)

Martin gained his PhD at Edinburgh Napier University in 2009, in Nanoparticle In Vitro Toxicolog. (swansea.ac.uk)
Martin's research is focused on the nanoparticle-(mammalian)cell interaction, with a view to determining the mechanistic toxicological, immunological and genotoxic effects that nanoparticles, with varying physico-chemical characteristics, may cause at the cellular level by using advanced, next-level in vitro systems combined with state-of-the-art microscopy and bioanalytical approaches. (swansea.ac.uk)

Culture media collected before and after 6 hours of stimulation of cells with phorbol 12-myristate 13-acetate were assayed to detect PGE 2 and PGF 2α via ELISA. (avma.org)

Giardia's Epithelial Cell Interaction In Vitro: Mimicking Asymptomatic Infection? (fu-berlin.de)
Endometrial cell death as a result of a T foetus infection is likely to be more important in mediating infertility than a direct effect on the conceptus. (avma.org)

However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (nih.gov)

Next-generation vaccines that utilize T cells could potentially overcome the limitations of current influenza vaccines that rely on antibodies to provide narrow subtype-specific protection and are prone to antigenic mismatch with circulating strains. (mdpi.com)
In the present study, for the first time, protective effects of three microalgal strains, including their extracts and active compounds, against both endogenous and exogenous AGEs in cell -based models were investigated. (rsc.org)

Epithelial cells are the primary site for carcinogenesis in most tissues, while stromal cells rarely develop into tumours. (uoguelph.ca)
Using RNA-Seq, we performed global transcriptomic profiling in primary NHBE cells exposed to diacetyl or 2,3-pentanedione. (nature.com)
Our data showed that diacetyl and 2,3-pentanedione induced significant transcriptomic changes, including those related to ciliogenesis, in the primary NHBE cells. (nature.com)
This review therefore, focuses on the functional as well as structural aspects of epithelial cells as well as the most commonly used in vitro epithelial models of the primary biological barriers with which nanomaterials might come in contact with either occupationally, or during their manufacturing and application. (unifr.ch)
Transfected HeLa cells were fixed in 3% paraformaldehyde, blocked with 0.2% Triton X-100, 1% FBS, 0.5% BSA in PBS with Ca[2+] and Mg[2+], followed by incubation in primary antibodies (anti-Myc mouse mAb, 9E10 (Covance) and anti-HA mouse mAb, HA.11 (Covance)) and secondary antibodies (Alexa 568 and Alexa 488) and DAPI (blue) to stain nuclei. (cellimagelibrary.org)

NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
The Miller laboratory is interested in the mechanisms by which viral pathogens manipulate host cell signal transduction pathways. (uc.edu)

The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. (nih.gov)
Serum components fibronectin and platelet-derived growth factor (PDGF) are strong chemoattractants for other RPE cells, astrocytes, and fibrocytes. (medscape.com)
For example, there is limited in vitro evidence that probiotic intervention with Streptococcus salivarius K12 might reduce growth of Candida . (medscape.com)

Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)

The Notch target, HES1, was present in mucus cells from all groups. (bmj.com)

2022) A community-based approach to image analysis of cells, tissues and tumors. (jax.org)

In addition, lung metastasis was observed in nude mice in T-16HBE-C1, 3 and 4 groups.In vitrocell migration assay results indicated that T-16HBE-C2?5 cells showed much lower migration capabilities than 16HBE cells. (eurekamag.com)
Results -Concentrations of PGF 2α and PGE 2 were significantly lower in culture media of adult and fetal endometrial epithelial cells exposed to any concentration of either CLA than they were in media of cells not exposed to CLAs. (avma.org)
Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)

Anatomy74

Organisms13

Diseases9

Chemicals and Drugs78

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes49

Disciplines and Occupations2

Information Science3

Mammary22

Bronchial epithelial5

Differentiation13

Airway4

Intestinal2

Alveolar Epithelial Cells2

Proliferation2

Apoptosis7

Bovine3

Mesenchymal14

Lung epithelial3

Branching lung1

Flow cytometry2

Human23

Pulmonary3

Phenotype3

Cytotoxicity1

Expression15

Endothelial2

Exposure2

Cultures1

Tumor2

Protein6

Mutagenicity1

Biology2

Stem12

Tight junction1

Induce1

Metabolic2

Epithelium2

Induction1

Vivo2

MUC5AC1

Organotypic3

Nanotoxicology1

Markers1

Tissue4

Undergo1

Nanoparticle2

Phorbol1

Infection2

Cellular Senescence1

Strains2

Primary5

Pathways2

Growth3

Peripheral1

Hes11

Tissues1

Results3