Vitro cell survival. Medical search. Frequent questions

Eliminating ROS elimination or inhibiting AKT blocked the effects of the laser therapy in vivo and in vitro. (google.com)
We have also compared the behavior of primary fetal cells to these human interneuron precursor-like cells both in vivo and in vitro. (ca.gov)
therefore, WS serves as a model for studying the aging process in vivo and in vitro. (medscape.com)

Gonocyte development consists of several phases of cell proliferation, differentiation, migration and apoptosis. (wikipedia.org)
This period consists of the primordial germ cells (PGC), the initial cells that commence germ cell development in the embryo, and the gonocytes, which after being differentiated from PGCs, undergo regulated proliferation, differentiation, migration and apoptosis to produce the SSCs. (wikipedia.org)
Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
Effects of RAD001 and letrozole (alone and in combination) on the proliferation and survival of MCF7/Aro and T47D/Aro cells were evaluated using proliferation assays, flow cytometry, immunoblotting, and apoptosis analyses. (aacrjournals.org)
This control of cDC homeostasis is mediated by a number of factors that drive cDC differentiation, proliferation, and survival or apoptosis ( 4 ). (frontiersin.org)
Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
Usually mutant cells are targeted for apoptosis (programmed cell death) but not always. (emfacts.com)
Apoptosis was determined by cell death assay and western blotting. (biomedcentral.com)
Triphala-mediated reduced cell survival correlated with induction of apoptosis, which was associated with reactive oxygen species (ROS) generation. (biomedcentral.com)
Triphala-induced apoptosis was linked with phosphorylation of p53 at Ser-15 and ERK at Thr-202/Tyr-204 in Capan-2 cells. (biomedcentral.com)
Pretreatment of cells with pifithrin-α or U0126, specific inhibitors of p53 or MEK-1/2, significantly attenuated Triphala-induced apoptosis. (biomedcentral.com)
Similarly, Triphala induced apoptosis in another pancreatic cancer cell line BxPC-3 by activating ERK. (biomedcentral.com)
On the other hand, Triphala failed to induce apoptosis or activate ERK or p53 in normal human pancreatic ductal epithelial (HPDE-6) cells. (biomedcentral.com)
Reduced tumor-growth in Triphala fed mice was due to increased apoptosis in the tumors cells, which was associated with increased activation of p53 and ERK. (biomedcentral.com)
Finally, EPB41L4A-AS2 inhibited breast cancer cell proliferation, migration and invasion and induced cell apoptosis in vitro. (cancerindex.org)

Infiltration, accumulation, and survival of chimeric antigen receptor T (CAR-T) cells in solid tumors is crucial for tumor clearance. (nature.com)
In mice, 7 × 19 CAR-T cells achieved complete regression of pre-established solid tumors and prolonged mouse survival, with superior anti-tumor activity compared to conventional CAR-T cells. (nature.com)
Following treatment of mice with 7 × 19 CAR-T cells, both recipient conventional T cells and administered CAR-T cells generated memory responses against tumors. (nature.com)
Kakarla, S. & Gottschalk, S. CAR T cells for solid tumors: armed and ready to go? (nature.com)
Enforced expression of the transcription factors PU.1, IRF8, and BATF3 (PIB) was sufficient to induce the cDC1 phenotype in 36 cell lines derived from human and mouse hematological and solid tumors. (lu.se)
Injection of in vitro generated melanoma-derived tumor-APCs into subcutaneous melanoma tumors delayed tumor growth and increased survival in mice. (lu.se)
Optimization encompassed increasing MGE cell motility, enhancing MGE cell maturation into inhibitory nerve subtypes, and elimination of tumors post-transplantation into the rodent brain. (ca.gov)
These melanocytic cells are of neuroectodermal origin, and melanocytic tumors may arise from these cells. (medscape.com)
Discovery of novel strategies to inhibit treatment-resistant metastatic tumors can increase survival and decrease side effects for high-risk neuroblastoma patients. (lu.se)
[ 17-20 ] In vitro and in vivo research suggests that NSAIDs and selective cyclooxygenase-2 inhibitors hinder growth and survival of bladder cancer cells and nitrosamine-induced tumors. (medscape.com)
Twelve miRNAs showed concordant expression in tumors vs. normal breast tissues and patient survival (n = 1093), with seven as potential tumor suppressors and five as potential oncomiRs. (cdc.gov)

Purified Goat IgG for use as a control in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and in vitro assays and as a standard in ELISA. (southernbiotech.com)
Clonogenic and cell viability assays were employed to determine cells survival curves from which the dose enhancement levels "radiosensitisation" are deduced. (medsci.org)
Combined with in-vitro proliferation assays using CRISPR-Cas9/RNAi and patient survival analysis, MEK inhibitors PD19830 and BRD-K12244279, pilocarpine, and tremorine were discovered as potential new drug options for treating breast cancer. (cdc.gov)

The second phase is spermatogenesis, which is a cycle of regulated mitosis, meiosis and differentiation (via spermiogenesis) leading to the production of mature spermatozoa, also known as sperm cells. (wikipedia.org)
Of the different cells isolated from the submandibular gland, this specific population, Lin - CD24 + c-Kit + Sca1 + , possessed the highest capacity for proliferation, self renewal, and differentiation during serial passage in vitro. (jci.org)
1. Harriman GR, Kunimoto DY, Elliott JF, Paetkau V, Strober W. The role of IL-5 in IgA B cell differentiation. (southernbiotech.com)
Towards the therapeutic development of inhibitory neuron precursor transplantation for human neurological disorders, we have made significant progress in the differentiation of these cells from human ESCs and will complete optimization of this protocol. (ca.gov)
During this first year, we have made considerable headway in the optimization of the human interneuron precursor differentiation protocol, verified functional engraftment of these cells in mice, and begun to collect dose, safety and efficacy data for rodent-derived interneuron transplantation. (ca.gov)
As we continue to optimize our ES cell differentiation protocol, these primary interneuron precursors will enable initial human cell dose response and behavior experiments and, along with rodent-derived cells, will provide important baseline measures. (ca.gov)

We intended to determine whether silymarin protects against H2O2-induced toxicity and affects survival rate of rat glioma (C6) and primary glial cells. (ogu.edu.tr)
Although silymarin dose and time dependence was more effective on decreasing glioma survival, it also reduced significantly primary glia survival. (ogu.edu.tr)
Interestingly, high Sprouty2 levels are also found in malignant glioma cells. (springer.com)

The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)
Locally advanced squamous cell carcinomas of the head and neck, (excluding lip) in combination with concurrent chemoradiation. (nih.gov)
[ 15 , 16 ] Although not expressed in normal urothelial tissue, cyclooxygenase-2 has been shown to be overexpressed in both transitional cell and squamous cell urothelial tumor tissue. (medscape.com)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (nih.gov)
a, arteriole), Aurora-A is massively detected in specific zones of G-IV, mostly around vessels (G-J). Notch4 is also detected in some tumor cells in addition to the endothelial lining of arterioles (H-K). (biomedcentral.com)
DAPI negative, single living cells were first separated with epithelial marker CD24 and hematopoietic and endothelial lineage marker CD45/31. (jci.org)
HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)
Human endothelial cells regulate survival and proliferation of human mast cells. (southernbiotech.com)
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. (ihcworld.com)
Vascular endothelial growth factor is a mitogen primarily for vascular endothelial cells. (ihcworld.com)
Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo. (ihcworld.com)

Histopathological analyses showed increased infiltration of dendritic cells (DC) and T cells into tumor tissues following 7 × 19 CAR-T cell therapy. (nature.com)
Dendritic cells (DCs) are specialized antigen presenting cells that instruct T cell responses through sensing environmental and inflammatory danger signals. (frontiersin.org)
Maintaining the homeostasis of the multiple functionally distinct conventional dendritic cells (cDC) subsets that exist in vivo is crucial for regulating immune responses, with changes in numbers sufficient to break immune tolerance. (frontiersin.org)
We used the minimal gene regulatory network of type 1 conventional dendritic cells (cDC1) to reprogram cancer cells into professional antigen-presenting cells (tumor-APCs). (lu.se)
In 2018, our research group identified the three transcription factors that allow us to convert fibroblasts, for example from the skin, into type 1 conventional dendritic cells (cDC1). (lu.se)

Restoring antitumor immunity with dendritic cell reprogramming strategies. (lu.se)
Alexandra Gabriela Barros Ferreira defends her Ph.D. thesis "Restoring antitumor immunity with dendritic cell reprogramming strategies" on Friday, 17 November, 2023. (lu.se)
My Ph.D. studies have focused on restoring anti-tumor immunity with dendritic cell reprogramming strategies. (lu.se)

The use of Survanta protection against silica (14808607) cytotoxicity in alveolar macrophages (AMs) was assessed in cells exposed to Survanta coated silica versus untreated silica. (cdc.gov)

In contrast, the typical median survival time after completion of first-line chemotherapy is just 15 months. (naturalmedicinejournal.com)
the median survival time from the first episode is only 1-4 months (2-11). (cdc.gov)

Here, we tested whether ALKBH6 plays a significant role in preventing alkylating DNA damage and decreasing genomic instability in pancreatic cancer cells. (frontiersin.org)
In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)
After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
Our preclinical studies demonstrate that Triphala is effective in inhibiting the growth of human pancreatic cancer cells in both cellular and in vivo model. (biomedcentral.com)
In the present study, we demonstrate that Triphala significantly inhibit the proliferation of Capan-2 and BxPC-3 human pancreatic cancer cells. (biomedcentral.com)

Development, Characterization and In Vitro Evaluation of Single o. (ingentaconnect.com)

Furthermore, treatment of s.c xenografts derived from A549 cells with intratumor injections of terpinen-4-ol significantly inhibited tumor growth compared with the control group. (hindawi.com)
A study comparing survival times among 341 epithelial ovarian cancer patients of whom 297 were non-diabetic, 28 were type II diabetics who did not use metformin, and 16 were type II diabetics who used metformin to find out if metformin use is associated with significantly longer progression free survival and overall survival in ovarian cancer patients. (naturalmedicinejournal.com)
In this study Romero et al report that metformin use was associated with significantly longer progression-free survival and overall survival in ovarian cancer patients receiving standard treatments. (naturalmedicinejournal.com)
Above mentioned effects were significantly blocked when the cells were pretreated with an antioxidant N-acetylcysteine (NAC), suggesting the involvement of ROS generation. (biomedcentral.com)
We recently demonstrated that abrogating Sprouty2 function strongly inhibits intracranial tumor growth and leads to significantly prolonged survival of glioblastoma bearing mice by induction of ERK-dependent DNA replication stress. (springer.com)

Clinical protocols for small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) were devised to prospectively select individualized chemotherapy based on in vitro drug sensitivity testing (DST) of cell lines derived from the patient's SCLC tumor cell lines or the patient's fresh NSCLC tumor. (elsevierpure.com)
0.05) with clinical response to primary therapy and also with response to the DST-selected chemotherapy regimen, but were not associated with survival (P = 0.24). (elsevierpure.com)
14%) in the absence of in vitro results to direct chemotherapy (P = 0.73). (elsevierpure.com)
The correlation between in vitro and clinical response was not significant for any individual drug or for all drugs considered together, illustrating the poor predictive value of in vitro testing with currently available chemotherapy in NSCLC. (elsevierpure.com)
Several alkylating agents are commonly used in chemotherapy but may induce DNA damage to cancer cells as well as normal cells. (frontiersin.org)
The aim of this study was to determinate the effect of different chemotherapy protocols used in the clinic on tumor stem cell markers in vitro . (isciii.es)
Type II diabetes diagnosis and metformin usage were compared with progression-free survival (PFS), overall survival (OS), and chemotherapy resistance rate. (naturalmedicinejournal.com)

We used VES and FBZ, at low concentrations, singly and in combination, to test their inhibitory effects on proliferation of human and mouse prostate cancer cells in vitro. (researchgate.net)

Finally, we will assess the feasibility of stem cell-derived dopaminergic micro-TENNs in vitro as a step towards "personalized constructs" and clinical translation. (michaeljfox.org)
Recent basic and clinical research has revealed that hydrogen is an important physiological regulatory factor with antioxidant, anti-inflammatory and anti-apoptotic protective effects on cells and organs. (researchgate.net)

This study provides fundamental insight into the functional impact of CDK4 inhibition on cells in the tumor microenvironment, which is of high importance and interest to the field. (elifesciences.org)
Our previous In Vitro studies demonstrated that simultaneous combination of imatinib with mitoxantrone yielded additive growth inhibition effects against PC-3 cell line. (ingentaconnect.com)
Our study presented for the first time an active loading method for imatinib and suggests that the optimized liposomal formulation co-encapsulates both drugs with high encapsulation efficiency (>95%), shows enhanced drug retention under tested conditions and delivers a drug:drug ratio capable of improving tumor cell growth inhibition with a mitoxantrone dose reduction of 2.6-fold as compared to single liposomal formulation. (ingentaconnect.com)
Thus, we conclude that As2O3 can reduce metastasis potential of NPC cells, involving inhibition of MMP-9 expression. (scielo.br)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)

Mechanistically, PTPN22 mediates cDC2 homeostasis in a cell intrinsic manner by restricting cDC2 proliferation. (frontiersin.org)

Next-generation micro-TENNs consisting of dopaminergic neurons and long unidirectional axonal projections will be characterized and optimize in vitro . (michaeljfox.org)

Later studies found that the process from primordial germ cell to spermatogonial development is gradual, without clear gene expression markers to distinguish the precursor cells. (wikipedia.org)

Importantly, we have achieved the development of a protocol that robustly generates interneuron-like progenitor cells from human ES cells and demonstrated that these progenitors mature in vitro and in vivo into GABAergic inhibitory interneurons with functional potential. (ca.gov)

High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. (nature.com)
Serial transplantations of this stem cell population into the submandibular gland of irradiated mice successfully restored saliva secretion and increased the number of functional acini. (jci.org)
These dosing and safety data will then serve as the baseline for comparison with human interneuron precursors and inform design of preclinical studies of these cells in immunosuppressed mice. (ca.gov)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

Developed as a commercial technology by DVS Sciences, which Fluidigm purchased in 2014, mass cytometry combines the capabilities of flow cytometry and atomic mass spectrometry, allowing it to measure large numbers of proteins in single cells with high throughput. (genomeweb.com)

Chondrolectin affects cell survival and neuronal outgrowth in in vitro and in vivo models of spinal muscular atrophy. (ox.ac.uk)

Gonocytes are functionally present during the first phase of germ cell maturation and development. (wikipedia.org)
Functionally, cDC1 cross-present exogenous antigens to activate CD8 + T cells and can promote IL-12 dependent Th1 responses ( 1 , 5 - 7 ). (frontiersin.org)
Furthermore, we demonstrated that the injected hPSC-MGE cells functionally matured into inhibitory nerves with advanced physiological properties that integrated into the rodent brain. (ca.gov)

After induction, hADSCs expressed von Willebrand Factor (vWF), the cell marker of ECs. (molvis.org)
We compared alternative procedures by testing MGE marker expression, and we developed additional tests to measure cell division and migration of MGE cell made from hESCs. (ca.gov)
For instance, the study found that while the marker PD-1 was broadly expressed across the observed T cell phenotypes, the markers TIM-3, CTLA-4, and 4-1BB were expressed only in certain cases. (genomeweb.com)
The study also identified a potential new marker for ccRCC immunotherapy, the protein CD38, which Chevrier said has been identified as a potential marker of T cell exhaustion in infectious disease, but has not been much discussed in the context of cancer. (genomeweb.com)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

However, the results of these treatments are poor and often exhibit no significant improvements in overall survival ( 8 ). (spandidos-publications.com)
Significant radiosensitisation (~67 %) of control was also noted in cells at megavoltage energies (commonly used in radiotherapy), compared to negligible levels detected by phantoms. (medsci.org)
Exposure of Capan-2 cells to the aqueous extract of Triphala for 24 h resulted in the significant decrease in the survival of cells in a dose-dependent manner with an IC50 of about 50 μg/ml. (biomedcentral.com)
In vitro cell culture models have evolved with significant improvements over several decades. (cdc.gov)

In Biology this is the type of cell division that results in two daughter cells each with half the chromosome number of the parent cell. (emfacts.com)

We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
14. Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction. (southernbiotech.com)
Under polarizing inflammatory conditions, cDC2 induce Th2 responses in the lung ( 9 , 10 ), drive Th17 responses through IL-23 secretion ( 11 - 17 ), and initiate SIRPα dependent induction of T follicular helper cells (T FH ) and germinal center (GC) formation ( 18 ). (frontiersin.org)

Cancer stem cells are responsible for tumor chemoresistance and recurrence in adjuvant and metastatic settings. (isciii.es)
We perform molecular and functional studies of primary and metastatic tumor cells, as well as in vitro and in vivo drug testing using PDXs as model system. (lu.se)

Depletion of recipient T cells before 7 × 19 CAR-T cell administration dampened the therapeutic effects of 7 × 19 CAR-T cell treatment, suggesting that CAR-T cells and recipient immune cells collaborated to exert anti-tumor activity. (nature.com)
These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
In vitro cell culture systems are instrumental in the development of therapeutic targets. (cdc.gov)

Spinal muscular atrophy (SMA) is characterized by the selective loss of spinal motor neurons owing to reduced levels of survival motor neuron (Smn) protein. (ox.ac.uk)
Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
To determine if instillation of Survanta would minimize or delay silica damage in rats, biochemical and cellular markers of lung injury were measured, including protein levels, beta- glucuronidase activity, total cells, and the number of neutrophils in bronchoalveolar lavage fluid (BALF) 1 and 14 days after instillation with silica and Survanta. (cdc.gov)
PBM-treated hypertrophic cells (1064 nm and 17.6 J/cm2 every day for 7 days following addition of PA) decreased the lipid levels in hypertrophic adipocytes, restored the GLUT4 protein expression and enhanced glucose transport. (google.com)
For example, researchers found a protein on the outside of several types of cancer cells in the 1990s. (mesothelioma.com)
That protein is now called mesothelin, and mesothelioma cells make a lot of it. (mesothelioma.com)
Using Fluidigm's Helios mass cytometry instrument, the researchers measured several dozen protein markers in 3.5 million immune cells, identifying 17 different tumor-associated macrophage (TAM) phenotypes and 22 T cell phenotypes. (genomeweb.com)
From experimentally validated target genes of these 86 miRNAs, pan-sensitive and pan-resistant genes with concordant mRNA and protein expression associated with in-vitro drug response to 19 NCCN-recommended breast cancer drugs were selected. (cdc.gov)

Dysregulation of in vitro cytokine production by monocytes during sepsis. (jci.org)
Plasma cytokines, cell-associated cytokines within freshly isolated monocytes and LPS-induced in vitro cytokine production were assessed at admission and at regular intervals during ICU stay. (jci.org)
Despite the fact that IL-1 alpha is the main cytokine found within monocytes upon in vitro activation of cells from healthy individuals, it was very rarely detected within freshly isolated monocytes from septic patients, and levels of cell-associated IL-1 beta were lower than those of TNF alpha. (jci.org)
Depending on the cytokine profile, cell death or survival pathways are activated, and these opposing outcomes can lead to inflammation through different mechanisms. (edu.au)

Our approach serves as a platform for the development of immunotherapies that endow cancer cells with the capability to process and present endogenous tumor antigens. (lu.se)
This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)
By using direct reprogramming strategies, I could induce the expression of tumor antigens on the surface of cancer cells, making them visible to the immune system again. (lu.se)
when it comes to cancer they are critical as they present tumor antigens to the killer cells of the immune system and orchestrate the anti-tumor immune response. (lu.se)

Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)

Micro-TENNs will then be transplanted into a neurodegenerative PD model to assess constructs survival, synaptic integration, dopaminergic functionality and PD symptom impact. (michaeljfox.org)
Advances in stem cell research and regenerative medicine have led to the potential use of stem cell therapies for neurodegenerative, developmental and acquired brain disease. (ca.gov)

Moreover, following injection of either the optimum dose or a 10-fold higher dose of mouse MGE cells, we found no detectable behavioral side effects from MGE cell transplantation. (ca.gov)

DST data derived from SCLC tumor cell lines were available for 33/115 (29%) patients. (elsevierpure.com)
Characterizing tumor cell lines. (helsinki.fi)

Decreased antigen presentation contributes to the ability of cancer cells to evade the immune system. (lu.se)
Reprogramming restored the expression of antigen presentation complexes and costimulatory molecules on the surfaces of tumor cells, allowing the. (lu.se)
In addition to acquiring improved antigen presentation, tumor-APCs had impaired tumorigenicity in vitro and in vivo. (lu.se)
We focused particularly on harnessing reprogramming, or direct cellular reprogramming, to induce antigen presentation in cancer cells. (lu.se)
Direct reprogramming strategies with cDC1-specific factors can induce antigen-presentation in cancer cells, making cancer cells visible to the immune system and easier to fight. (lu.se)

The compelling conclusion that proliferative exhausted T cells are associated with response in HPV+ head and neck cancer is supported by the cohort of 14 patients with paired tumor and adjacent normal tissue and rigorous bioinformatic analysis of nearly 50,000 single CD3+ T cell transcriptomes. (elifesciences.org)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
is the term applied to laboratory techniques using cell-cultures (tissue culture), as distinct from those using human subjects or live animals for testing possible harmful products. (emfacts.com)

The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)
In the case of T cells, the researchers used a panel consisting largely of canonical T cell markers providing an overview of the T cell phenotype landscape and proteins known to be relevant to immunotherapy. (genomeweb.com)
Then we used an antibody screen to determine which markers were most informative for distinguishing between the [different] macrophages that we were able to generate in vitro ," he said. (genomeweb.com)
One key takeaway from the data, he said, was the fact that T cells "are heterogeneous in the expression of what people think of as the markers that should be targeted in immunotherapy. (genomeweb.com)

Male fertility is centered around continual spermatogonia which is dependent upon a high stem cell population. (wikipedia.org)
Here, we identified and characterized a stem cell population from adult murine submandibular glands. (jci.org)

Published this week in Cell , the study used mass cytometry to characterize T cells and macrophages in samples from 73 ccRCC patients and five healthy matched kidney samples, identifying immune phenotypes associated with progression free-survival as well as potential biomarkers that could be useful for purposes including immunotherapy development. (genomeweb.com)

Administration of GDNF improved saliva production and enriched the number of functional acini in submandibular glands of irradiated animals and enhanced salisphere formation in cultured salivary stem cells, but did not accelerate growth of head and neck cancer cells. (jci.org)
Neonatal murine epidermal cells express a functional multidrug-resistant pump. (southernbiotech.com)
For the functional screening, Bcl2 - and Myc- expressing primary B cells were infected with a retroviral cDNA library. (haematologica.org)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)

Rapid clearance and reduced cell entry thus lead to decreased amount of oncolytic adenovirus in target cells and decreased efficacy. (helsinki.fi)

P-Tex cells could aggregate in the antigen-presenting cell niches and activate certain signaling pathways. (elifesciences.org)
Our existing knowledge of SARS-CoV-2 driven disease predominantly relies on correlative or in vitro studies, and it is yet unclear which pathways are causative of severe inflammation in vivo . (edu.au)
NF-kB or similar pathways increase the survival and growth of mesothelial cells. (mesothelioma.com)

evidence of a novel autocrine survival factor in AML. (ox.ac.uk)
VEGF regulates haematopoietic stem cell survival by an internal autocrine loop mechanism. (ihcworld.com)

Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)
The A549 human lung adenocarcinoma and CL1-0 lung adenocarcinoma cell lines were cultured in Dulbecco′s modified eagle medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic antimycotic. (hindawi.com)
We used the pancreatic adenocarcinoma cell line PANC-1 as a model to apply different chemotherapeutic protocols (monotherapy and combined therapy) using 5-Fluorouracil, Oxaliplatin, Irinotecan, Gemcitabine and Abraxane. (isciii.es)

This involves the study of mesothelioma cells and proteins. (mesothelioma.com)
However, cDC1 can be compromised in cancer patients and some cancer cells lack these antigen-presenting proteins on their surface, impacting immunotherapy response. (lu.se)

Fibroblastic reticular cells in lymph nodes regulate the homeostasis of naive T cells. (nature.com)

Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
Upon LPS stimulation, we observed a profound decrease of in vitro IL-1 alpha production by monocytes in all patients, and of IL-1 beta, IL-6, and TNF alpha in septic patients. (jci.org)

2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Gonocytes are the precursors of spermatogonia that differentiate in the testis from primordial germ cells around week 7 of embryonic development and exist up until the postnatal period, when they become spermatogonia. (wikipedia.org)
The gonocyte population develops from the post migratory germ cells (PGCs) around embryonic day (ED) 15. (wikipedia.org)
In this reporting period, we continued to improve the acquisition of migratory medial ganglionic eminence (MGE)-type interneurons from human embryonic stem cells (hESC). (ca.gov)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

Genetic alterations of BCL2 (B-cell CLL/lymphoma 2), MYC [v-myc myelocytomatosis viral oncogene homolog (avian)] and CCND1 (cyclin D1) are the most frequently found alterations in human B-cell lymphomas. (haematologica.org)
One vial containing 420 mg of trastuzumab, a humanised IgG1 monoclonal antibody produced by mammalian (Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)

Furthermore, in silico analysis from The Cancer Genome Atlas (TCGA) database suggests that overexpression of ALKBH6 provides better survival outcomes in patients with pancreatic cancer. (frontiersin.org)
A proliferative exhausted CD8 + T cell cluster (P-Tex) which was beneficial to survival outcomes of patients with HPV-positive HNSCC was identified. (elifesciences.org)

For instance, patients with larger presence of the M-11 and/or M-13 phenotype as well as a smaller presence of the M-5 phenotype had shorter progression-free survival. (genomeweb.com)

The study compared survival times among 341 epithelial ovarian cancer patients, of whom 297 were non-diabetic, 28 were type II diabetics who did not use metformin, and 16 were type II diabetics who used metformin. (naturalmedicinejournal.com)

Anatomy20

Organisms14

Diseases31

Chemicals and Drugs72

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Phenomena and Processes59

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture4

Information Science5

Health Care20

Vivo and in vitro3

Apoptosis20

Tumors11

Assays3

Differentiation6

Glioma3

Squamous Cell3

20002

Endothelial8

Dendritic cells5

Immunity with dendritic cell reprogramm3

Cytotoxicity1

Median survival time2

Pancreatic cancer cells7

Characterization1

Significantly5

Chemotherapy7

Proliferation of human1

Clinical2

Inhibition5

Mechanistically1

Optimize1

Precursor cells1

Progenitor cells from human1

Mice4

Flow Cytometry1

Neuronal1

Functionally3

Marker5

Significant4

Biology1

Induction4

Metastatic2

Therapeutic3

Protein8

Cytokine4

Antigens4

Stimulates1

Neurodegenerative2

Injection1

Tumor cell lines2

Antigen presentation5

Tissue3

Markers4

Stem cell population2

Macrophages1

Functional4

Efficacy1

Pathways3

Autocrine2

Adenocarcinoma3

Proteins2

Regulate1

Decrease2

Humans1

Embryonic4

Viral2

Outcomes2

Phenotype1

Epithelial1