Vitro cell culture techniques. Medical search. Frequent questions

Qualitative and quantitative analyses of the rare phenotypic variants in in vitro culture systems is necessary for the understanding of cell differentiation in cell culture of primary cells or cell lines. (nih.gov)
We found that myocilin is expressed in bone marrow-derived mesenchymal stem cells (MSCs) and affects their differentiation into osteoblasts. (nih.gov)
Bioreactors provide controlled microenvironments and nutrient delivery to regulate cell growth and differentiation, improving standardization and reproducibility. (sigmaaldrich.com)
Ongoing and future applications include 100-port valves and systems for optimizing the differentiation of induced pluripotent stem cells, a 10,000 channel yeast MicroChemostat, and a MicroDialysis imager, all with direct injection into a mass spectrometer. (selectbiosciences.com)
Using a defined in vitro model of early chondrogenesis, we attempted to enrich for cells with an enhanced ability for chondrogenic differentiation by pre-exposure of mouse adipose-derived adult stromal cells (ADASs) to a hypoxic (2% oxygen) environment. (ox.ac.uk)
ADASs were subsequently expanded in 2% or 21% oxygen environments, resulting in 2 groups of cells, and then early chondrogenic differentiation was induced at 21% oxygen tension using a 3-dimensional micromass culture system. (ox.ac.uk)
Differentiation of hASCs was induced by using the micromass culture technique. (utwente.nl)
The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)
Neuronal and glial differentiation within expanded glial cultures derived from the lateral and medial ganglionic eminences. (lu.se)
Neuronal differentiation following transplantation of expanded mouse neurosphere cultures derived from different embryonic forebrain regions. (lu.se)
Through the use of human embryonic stem cells (hESCs), we can mimic brain development towards different regions of the human brain, and thereby investigate the effect of novel genes on neural differentiation. (lu.se)
function of proliferative responses provoked in the induction cell products favor immediate type hypersensitivity reactions, phase of contact allergy, the murine local lymph node assay serving as growth and differentiation factors for mast cells and (LLNA), has been accepted as a stand-alone method for skin eosinophils, the cellular effectors of the clinical manifestations of sensitization testing (NIH, 1999). (cdc.gov)

In vitro and in vivo experiments were performed following sonication of BNNTs in dispersion media (DM). (cdc.gov)
Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
Together these techniques may help to better describe the behaviour of nanoparticles when used for cell culture experiments. (spie.org)
This resistance was not due to differences in the cell cycle state of these inner cells since separate experiments showed that both exponential and plateau phase monolayer cells were about equally sensitive when the surviving fraction was plotted vs absorbed drug (D0, = 0.04 μ/106 cells). (researchgate.net)
We present you 3 practical solutions on how to improve your 3D spheroid in vitro experiments. (researchgate.net)
This is why some experiments are conducted using 3D cultures, which can be grown using scaffold-based or scaffold-free techniques. (integra-biosciences.com)
Despite the different approaches and techniques, all experiments have one thing in common: it's difficult to grow viable cells in the desired quantity to obtain reproducible results. (integra-biosciences.com)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Further viability experiments showed an irreversible cell damage, since no recovery occurred after up to 71 h in YTX-free medium. (units.it)
We published a critique of the state of the art concerning temperature mapping in living cells, questioning the validity of recent experiments [20,22]. (fresnel.fr)
Experimental pharmacological methods are introduced, including operation techniques and in vitro experiments with excised tissues or organs. (lu.se)
Meta-analyses inclusive of IAV that display a diverse range of phenotypes in ferrets, interpreted with mathematical modeling of viral kinetic parameters, can provide critical information supporting a more rigorous and appropriate contextualization of in vitro experiments toward pandemic preparedness. (cdc.gov)

Cytosine arabinoside (AraC) 10 μ M was added to the media the last 2 days to prevent proliferation of other glial cells. (hindawi.com)
The protein surface coating provides improved adhesion and proliferation of cells in vitro. (prnewswire.co.uk)
We discovered that myocilin increased cell proliferation and survival. (nih.gov)
EndoECM supported in vitro culture of human endometrial cells in two- and three-dimensional conditions and improved proliferation of endometrial stem cells with respect to collagen and Matrigel. (datadryad.org)
Uncover its applications in cell viability testing, cytotoxicity, cell proliferation, and high throughput screening services. (acmeresearchlabs.in)
Cytotoxicity Test by MTT Assay- MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test is used to measure the cellular metabolic activity in mammalian cells as an indicator of cell viability, proliferation and cytotoxicity. (acmeresearchlabs.in)
Characterization of TSP-4 expression under development and pathogenesis of disorders has yielded important insights into mechanisms underlying the unique role of TSP-4 in mediating various processes including cell-cell, cell-extracellular matrix interactions, cell migration, proliferation, tissue remodeling, angiogenesis, and synaptogenesis. (bvsalud.org)
The results showed that the growth curve for cell proliferation and the formation of mineral nodules was similar for both cultures analyzed. (bvsalud.org)
1994. Mesothelial cell proliferation: A nonspecific response to lung injury associated with fibrosis. (cdc.gov)

Primary cultures of neocortical neurons were obtained from 16-day-old NMRI mouse embryos (Charles River, Iffa Credo, Saint-Germain-sur-l'Arbresle, France) [ 5 , 6 ]. (hindawi.com)
The aim of this Challenge was to generate an approach that improves the implantation rates of early stage embryos when combined with extended in vitro culture and non-surgical embryo transfer techniques. (nc3rs.org.uk)
The team at University of Leeds led by Dr Virginia Pensabene has developed a novel and reliable microfluidic device that improves the developmental competence of in vitro -derived mouse embryos to allow the use of non-surgical embryo transfer (NSET) in the generation of transgenic mice. (nc3rs.org.uk)
The team at University of Leeds led by Dr Virginia Pensabene has developed a novel and reliable microfluidic device that improves the developmental competence of in vitro -derived mouse embryos and their implantation potential, enabling the use of non-surgical embryo transfer (NSET) in the generation of transgenic mice. (nc3rs.org.uk)
Effects of Uterine Cells-Conditioned Media on Expression of DNMT3B and DNMT3C in Mouse Embryos Cultured in a Microfluidic Device. (nc3rs.org.uk)
Sponsored by MRC Harwell, the EASE Challenge aims to generate an approach that improves the implantation rates of early stage embryos when combined with extended in vitro culture and non-surgical embryo transfer techniques. (nc3rs.org.uk)
The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)
In embryo co-culture, embryos are grown in "feeder layers" of cells from the patient that enhance development. (ucsfhealth.org)
The assisted hatching technique was introduced to enhance the embryos' ability to hatch, and thus implant, after transfer. (ucsfhealth.org)
Blastocyst culturing is a technique to grow embryos beyond the third day of culture. (ucsfhealth.org)
On the third day, embryos generally are between six to eight cells. (ucsfhealth.org)
Over the years, improved culture environments have allowed us to culture the embryos two additional days. (ucsfhealth.org)
During this additional culture period, the embryos continue to grow to become "blastocysts. (ucsfhealth.org)
In certain patients, blastocyst culturing allows optimal selection of embryos for transfer, resulting in an increased implantation rate per embryo transferred. (ucsfhealth.org)
As patients get older, fewer and fewer numbers of embryos are capable of developing in culture to the blastocyst stage. (ucsfhealth.org)
Embryo co-culturing is a technique used to improve the overall quality of embryos before transferring them into the mother's uterus. (ucsfhealth.org)
For his doctoral thesis, Dr. Brinster developed the first reliable in vitro culture system for early mammalian embryos. (avma.org)
Today, this technique continues to form the foundation for research on mammalian embryos, including technologies such as transgenic engineering, embryonic stem cell therapy, human in vitro fertilization, mammalian cloning, and knockout engineering. (avma.org)
From there, Dr. Brinster became interested in modifying the development of animals and their germ lines, and he went on to become the first person to show that it was possible to colonize a mouse blastocyst with stem cells from older embryos. (avma.org)
After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
Results: The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cell embryos (p=0.04). (who.int)
As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
Assisted reproductive techniques (ARTs) involve manipulation of sperm and ova or embryos in vitro with the goal of producing a pregnancy. (msdmanuals.com)

TNT cellular networks are upregulated between cancer and stromal cells under hypoxic and other conditions of physiologic and metabolic stress. (frontiersin.org)
In vitro expansion of adipose-derived adult stromal cells in hypoxia enhances early chondrogenesis. (ox.ac.uk)
Recent advances in isolating epithelial progenitor cells and stromal cells, as well as defining niche factors important for lung development, have led to the establishment of an in vitro 3D lung culture system: pulmonary (lung) organoids. (stemcell.com)
Recent progress isolating lung epithelial progenitor and stromal cells, as well as defining stem cell niche factors that are important for lung development, has led to the establishment of an in vitro three-dimensional (3D) culture system: lung organoids 2 , also known as airway organoids or pulmonary organoids. (stemcell.com)
The lung is a complex organ composed of many types of epithelial cells, immune cells, endothelial cells and stromal cells. (stemcell.com)
Further, we developed a three-dimensional endometrium-like co-culture system of epithelial and stromal cells from different origins. (datadryad.org)

Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
We are interested in understanding how these modifications are misregulated in tumor cells, tumor-infiltrating immune cells, and tumor-interacting stroma cells. (postdocjobs.com)

The metabolic and developmental impact of murine embryo culture in a novel microfluidic device. (nc3rs.org.uk)
On-chip mouse embryo culture: evaluation of effects of uterine cells-conditioned media on embryo development and gene expression. (nc3rs.org.uk)
A team led by Dr Virginia Pensabene from the University of Leeds has been awarded £95,883 to deliver the project: Design, Fabrication and Testing of a Mouse Embryo Culture Chip. (nc3rs.org.uk)
In addition to standard IVF procedures - including intracytoplastmic sperm injection , assisted hatching, and blastocyst culturing - we offer a more unique technique, called embryo co-culture. (ucsfhealth.org)
This technique appears to improve embryo growth for patients who have had poor embryo development and unsuccessful IVF cycles. (ucsfhealth.org)
Background: The success of in vitro-fertilization (IVF) cycles is determined in large part by the quality of embryo cleavage, which in turn, is dependent on the quality of the embryo culture media (CM). Many factors can influence the quality of embryo CM, one of which is the levels of Cell Free Deoxyribonucleic acid (DNA). (who.int)
Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
Methods: This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
Conclusion: We conclude that cell-free DNA levels in CM might be associated with delayed embryo cleavage. (who.int)
Jamil M., Debbarh H., Jelloul H., Kabit A., Ennaji M., Zarqaoui M., Hissane E. M., Senhaji W., Louanjli N., Cadi R.. Cell-free DNA in Embryo Culture Media as Non-invasive Biomarker of the Quality of Embryo Cleavage. (who.int)
For assisted reproductive techniques, oocytes and sperm are collected from the intended parents or donors, and an embryo or the gametes are transferred to the woman's reproductive tract after culture in vitro. (msdmanuals.com)

The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS). (sigmaaldrich.com)
Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Molecular biological methods, cell culture and tissue culture are treated to a less extent. (lu.se)
We also work on more sophisticated 3D culturing methods to model human brain development on an anatomical level with hESCs. (lu.se)

Martin's research is focused on the nanoparticle-(mammalian)cell interaction, with a view to determining the mechanistic toxicological, immunological and genotoxic effects that nanoparticles, with varying physico-chemical characteristics, may cause at the cellular level by using advanced, next-level in vitro systems combined with state-of-the-art microscopy and bioanalytical approaches. (swansea.ac.uk)
Another clear ambition of Martin's research is to create, study and promote alternative in vitro models that mimic in vivo environments, in order to gain a mechanistic understanding of how nanoparticles may, potentially, adversely impact human health. (swansea.ac.uk)
Special focus is also given to creating disease-based in vitro models and the role nanoparticles play in disease onsert and/or exacerbation. (swansea.ac.uk)
We study problems in thermal biology at the level of single cells generated by heating of gold nanoparticles, such as the heat-shock response of single living cells [30]. (fresnel.fr)
Many experimental techniques are available: from standard instrumentation for size and concentration measurements of airborne nanoparticles to more specialized equipment. (lu.se)

In this paper we develop a method that applies these principles to a particularly hot problem in cell biology, the study of stem cell like cells in cultures of primary cells, cancer cells, and various cell lines. (nih.gov)
For the quality assurance you need for molecular biology and cell culture, Corning ® Costar ® Stripette ® or KIMAX ® 51 serological pipettes are hard to beat. (sigmaaldrich.com)
It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)
In Biology this is the type of cell division that results in two daughter cells each with half the chromosome number of the parent cell. (emfacts.com)
In addition, he holds a secondary appointment as Professor of Cell and Developmental Biology and serves as staff physician at the Nashville Veterans Medical Center. (vumc.org)
Dr. Goldenring previously served as Professor of Medicine, Surgery and Cellular Biology and Chief, Program in Cell Signaling, Institute of Molecular Medicine and Genetics at the Medical College of Georgia in Augusta GA and staff physician and Director, Laboratory of Molecular Gastroenterology Augusta Veterans Medical Center. (vumc.org)
The lab uses a wide range of approaches in our projects: molecular biology, protein biochemistry, in vitro cell culture models, xenograft and syngeneic mouse models, genetically engineered mouse models, various CRISPR-based techniques, and genomic/transcriptomic/proteomic omics techniques. (postdocjobs.com)
Just because any area of science features thermal-induced processes (thermodynamics, chemical synthesis, fluid dynamics, phase transitions, cell biology, etc), the possible systems of interest that our technique can address is countless, with only one limitation : the imagination. (fresnel.fr)
So far, thanks to this microscopy technique, we have been able to address problems in physics, chemistry and cell biology. (fresnel.fr)

Cell Reports 2022. (postdocjobs.com)
In 2022 the laboratory installed two new advanced and world-unique chambers by combining techniques from thermal climate and aerosol research. (lu.se)

An imbalance in protein homeostasis (proteostasis) can result in severe molecular damage to the cell, directly leading to tissue pathology and to enhanced susceptibility to diseases not only including metabolic disease and neurodegeneration, but also cancer and immunodeficiency. (uni-bremen.de)
Most widely used technique for quantitative molecular analysis for the identification and structural analysis of organic compounds. (sctimst.ac.in)
This research provides a way to predict the regions where the next new emerging zoonoses from bats is most likely to emerge, and proposes targeted surveillance of these animals using state-of-the- art molecular techniques in those regions. (hhs.gov)
At these levels and everything in between, work is carried out using an enormous range of approaches such as gene arrays, molecular techniques, cell cultures, tissue samples, computer models, animal studies, and human studies. (ox.ac.uk)
Invadopodia are F-actin-rich protrusions that form on the advancing edge of cells, supported by complex molecular interactions at the cell membrane. (bvsalud.org)
Viral titers in ferret nasal wash specimens and nasal turbinate tissue correlated positively with peak titer in Calu-3 cells, whereas additional phenotypic and molecular determinants of influenza virus virulence and transmissibility in ferrets varied in their association with in vitro viral titer measurements. (cdc.gov)

4 In cell culture assays, a large proportion of the reproducibility issues come from biological variation between passages or generations of cells. (integra-biosciences.com)
Unlike multiplex or ELISA assays, the HEp-2 cell substrate provides 100-150 possible autoantigens to detect ANAs with greater sensitivity than the solid phase assays. (nemours.org)
In Vitro Assay Services in Dehradun: In the burgeoning field of biotechnology and pharmaceuticals, in vitro assays have become instrumental in our understanding of biological processes and drug development. (acmeresearchlabs.in)
Nineteen national-level public health laboratories performed routine dengue diagnostic assays on a proficiency testing panel consisting of two modules: one containing commercial serum samples spiked with cultured dengue viruses for the detection of nucleic acid and non-structural protein 1 (NS1) (Module A) and one containing human serum samples for the detection of anti-dengue virus antibodies (Module B). A review of logistics arrangements was also conducted. (who.int)

We use cell culture techniques, mutagenesis approaches, primary cultures of neurons and glia, pharmacology, in vitro models of stroke and epilepsy, and in vivo models of neuropathic pain and cocaine abuse disorder. (drexel.edu)
Understanding invadopodium formation and function in cancer cells is therefore an important strategy to find novel clinical approaches to interfere with metastasis. (bvsalud.org)
This will be coupled with next generation sequencing based approaches to understanding the consequences of perturbing immune cell function. (lu.se)
These analyses provide a necessary first step in enhanced interpretation and incorporation of in vitro-derived data in risk assessment activities and highlight the utility of employing mathematical modeling approaches to more closely examine features of virus replication not identifiable by experimental studies alone. (cdc.gov)
However, with a growing number of novel cell sources (stem cells and reprogrammed cells) it becomes important to develop new methodologies for a more thorough assessment of human neurons generated using these approaches. (lu.se)

In vitro fertilization (IVF) involves fertilization outside the body in an artificial environment. (ucsfhealth.org)

This study was performed on neonatal rat cardiomyocytes to study toxin effects on various fundamental aspects of cardiac muscle cells activity: cell beating, intracellular Ca2+ and cAMP levels, cell vitality, mitochondrial membrane potential and type of cell death occurrence. (units.it)
These results show a very cell-specific response to YTX if compared to previous studies, and a severe damage to in vitro cardiomyocytes. (units.it)

Thus, other factors related to the metabolic state of the cells, the microenvironment, or the formation of different drug products must account for the observed resistance. (researchgate.net)
Our current projects include the multifaceted roles of ITCH in melanoma and the melanoma immune microenvironment, the context-dependent functions of master cell cycle regulator APC/C in breast cancer and ovarian cancer, the crosstalk between tumor cell aneuploidy and innate immunity, and several others. (postdocjobs.com)

Decellularization techniques support the creation of biocompatible extracellular matrix hydrogels, providing tissue-specific environments for both in vitro cell culture and in vivo tissue regeneration. (datadryad.org)
3D cell culture allows you to more accurately model in vivo tissue under in vitro conditions. (eventscribe.net)

Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Organ-on-chips are miniaturized devices that arrange living cells to simulate functional subunits of tissues and organs. (selectbiosciences.com)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)

Primary cultures of cortical neurons and astrocytes were treated with EO (0-25%) for up to 90 min. (hindawi.com)
The cell suspension for astrocyte culture was obtained in the same way as for neurons. (hindawi.com)
This also allows us the unique opportunity to directly compare our hESC-derived dopamine neurons with those sourced from human fetal tissue both in vitro and in vivo. (lu.se)
We utilise monosynaptic tracing technology, using pseudotyped rabies vectors, in order to visualise the synaptic contacts formed between host neurons and graft human cells. (lu.se)
The cells are called induced neurons (iNs) and offer a shortcut over induced pluripotent stem cells (iPSc) for generating patient and disease-specific neurons. (lu.se)
Current efforts focus on identifying the optimal starting cell population for neural conversion, identification of gene combinations that yield subtype specificity and analysis of function and integration of the reprogrammed neurons. (lu.se)

Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
This process relies on the formation of a blastema, a pool of progenitor cells that is formed in response to amputation. (lu.se)

Engineering cartilage has typically involved 'encapsulating chondrocytes, or stem cells which can be differentiated along a chondrogenic linage, in a supportive matrix such as a hydrogel or scaffold,' Advanced Healthcare Materials explains. (corning.com)
The main aim of this research project was to regenerate AF tissue in vitro using hASCs and serum free chondrogenic medium without supplementation of growth factors. (utwente.nl)
Quantification of sulphated glycosaminoglycan (GAG) content showed that hASCs cultured in chondrogenic medium expressed similar levels of sulphated GAGs as hAFCs. (utwente.nl)

Research and Markets has announced the addition of the "Cell Culture Protein Surface Coating Market Analysis By Coating Type (Self-Coating, Precoating [Multiwall/Microwell Plates, Petri Dishes, Flasks, Slides, Cover Slips]), Protein Source (Plant, Animal, Human, Synthetic) And Segment Forecast 2014-2025" report to their offering. (prnewswire.co.uk)
The cultures are grown in a sterile synthetic liquid medium devoid of enriched supplements such as serum. (cdc.gov)

Furthermore, by creating sophisticated in vitro multi-cellular models of different human organs in combination with state-of-the art microscopic and biochemical analyses, Martin is able to obtain a mechanistic understanding of the health risks posed by nanomaterials under realistic conditions, as well as gain valuable insight into the potential of in vitro as alternative test systems to in vivo methodologies. (swansea.ac.uk)

One set was tested by culture techniques for the presence of live organisms at the Mycoplasma Laboratory, Science Applications International Corporation, National Cancer Institute, Frederick Research and Development Center, Frederick, MD. A commercial nongovernment facility, Charles River Tektagen, tested the second set for mycoplasma DNA by polymerase chain reaction (PCR) assay. (cdc.gov)
For example, after 1 hr exposures to 0.5 μ/ml, surviving fractions determined by colony formation assay were approximately 0.3 for cells from spheroids treated intact and 0.001 for single exponential phase cells. (researchgate.net)
The immunofluorescence ANA assay (Antibodies, Inc) utilizes the method considered the gold standard for ANA testing by the American College of Rheumatology: detecting circulating autoantibodies reactive with nuclear antigens of HEp-2 cells. (nemours.org)
Explore the pivotal role of in vitro MTT Assay in drug discovery and biotechnological research. (acmeresearchlabs.in)
An in vitro cytotoxicity study is an assay to determine the effect of a substance on cells in a culture. (acmeresearchlabs.in)

Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
Six parasites showed in vitro resistance to sodium stibogluconate using murine J774 macrophage amastigote testing method. (who.int)

Cells were grown for 2 weeks in DMEM : F12 containing 10% fetal bovine serum (FBS) until reaching confluence. (hindawi.com)

Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
The technology can mimic cartilage's natural mechanical properties, and it can be bioprinted to engineer 3D structures with cells along gradients and more complex biological cues. (corning.com)
In this project, involving engineers from LTH, we apply advanced microfluidic techniques to culture hESCs under the influence of chemical gradients to mimic the environment around the developing brain in the fetus, thereby generating neural tissue with anatomical characteristics resembling the developing human brain. (lu.se)

Tight junctions between adjacent epithelial cells constitute the physiological barrier to fluid and macromolecules in epithelium. (nih.gov)
2021). Metabolomic Analysis Evidences That Uterine Epithelial Cells Enhance Blastocyst Development in a Microfluidic Device. (nc3rs.org.uk)
The alveolar epithelium is lined with type I and II alveolar epithelial cells (AECs). (stemcell.com)

Although these cells represent only about 1% of the total population as calculated by flow cytometry, they can be identified in the culture plate with high precision by microscopy. (nih.gov)
Our major contribution to this field of research (named thermoplasmonics) was to develop a unique temperature microscopy technique capable of mapping the temperature and the heat source density throughout plasmonic structures under illumination [8]. (fresnel.fr)
Cell culture, primary brain cultures, microscopy. (lu.se)

Considering that PTZ blocks the chloride channel coupled to the GABA A receptor complex, the present study aimed to analyze the possible modulation of GABAergic homeostasis within synaptic clefts in vitro . (hindawi.com)
The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)
In this study, nine commercially available, chemically defined media and feed combinations from different vendors were assessed for producing monoclonal antibody in Chinese hamster ovary (CHO) cells. (sigmaaldrich.com)
This study is relevant for the future application of cell-based therapies involving cartilage tissue regeneration. (ox.ac.uk)
Slow Food is deeply alarmed by the European Commission's conclusions from the study on "new genomic techniques" which opens the door to the deregulation of new GMOs, ignoring the precautionary principle. (gmwatch.org)
Today, the European Commission released its long-awaited study on new genomic techniques, which was requested by the Council of the EU in November 2019. (gmwatch.org)
The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. (scielo.br)
The majority of Oxford's medical research is carried out using either in vitro techniques or the study of human beings. (ox.ac.uk)
5 demonstrated, in a study carried out using osteoblastic cells, that bisphosphonates do not cause cytotoxic action when administered to the cell culture in the period between 24 and 72 hours, and are capable of reversing the harmful effects of glycation in osteoblasts in patients with osteoporosis and diabetes mellitus. (bvsalud.org)
1984. Fibrogenesis by mineral fibres: An in-vitro study of the roles of the macrophage and fibre length. (cdc.gov)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)

Moreover, Dr. Brinster first demonstrated that teratocarcinoma cells could combine with blastocyst cells to form adult chimeric mice, establishing the feasibility of this approach to change the genetic character of mice. (avma.org)

pregnancy rates with this technique are lower than those with retrieval of multiple oocytes, but costs are lower and success rates are increasing. (msdmanuals.com)
The oocytes are inseminated in vitro. (msdmanuals.com)
The semen sample is typically washed several times with tissue culture medium and is concentrated for motile sperm, which are then added to the medium containing the oocytes. (msdmanuals.com)
After sperm are added, the oocytes are cultured for about 2 to 5 days. (msdmanuals.com)

Using a physiologically relevant air-liquid interface exposure system, electrically tight monolayers of the human bronchial epithelial cell-line Calu-3 were exposed to fresh, whole mainstream cigarette smoke. (nih.gov)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)

Could tissue engineering and 3D cells create functional replacement tissue for joints damaged by osteoarthritis? (corning.com)
Pioneering tissue engineering studies used traditional 2D substrates, but in recent years, research has focused on the development of biomimetic 3D scaffolds and cell culture platforms to repair and regenerate osteoarthritis' osteochondral defects, according to Bio-Design and Manufacturing . (corning.com)
Osteochondral tissue engineering is the additive technology that 'makes it possible to spatially pattern cells, bioactive factors, and biomaterials in 3D,' Advanced Healthcare Materials asserts. (corning.com)

The in vitro and ex vivo culture and maintenance of parasites involves highly complex procedures, which are subject to many variables. (hindawi.com)
The typical seed train cell expansion process involves thawing cryopreserved cells followed by successive expansions into larger culture vessels including flasks, spinners, and stirred bioreactors. (sigmaaldrich.com)
Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)

The images of cell populations were collected by a two-photon microscope and processed by a software developed by us. (nih.gov)
Studies in the laboratory focus on how immune cell populations (M2-macropahges and IL-C2 cells) regulate the induction of metaplasia and its progression to more aggressive and proliferative pre-cancerous lesions. (vumc.org)
Other studies are seeking to identify the characteristics of pre-cancerous stem cell populations using metaplastic and dysplastic organoid lines isolated from both mouse models and human patients. (vumc.org)

Slide-based cytometry combines image acquisition and data treatment, and associates the power of flow cytometry (FCM) and the resolution of the microscopic studies making it suitable for the analysis of cells with rare phenotype. (nih.gov)
Since the fluorescence intensity of rare events and the position of thousands of surrounding cells are recorded at the same time, the method associates the advantage of the FCM analysis and the microscopic observation. (nih.gov)
Invadopodia formation, structure, and function can be studied in vitro, using commonly cultured cancer cell lines and standard microscopic techniques. (bvsalud.org)
And my luck was that I entered brain research at the time, in the late 1960s, when modern neuroscience was born, and in the labora- tory where a new groundbreaking microscopic technique, the Falck-Hillarp monoamine histofluorescence method, had just been developed. (lu.se)

Oppositely, expression of mutated myocilin sensitizes cells to apoptosis induced by oxidative stress. (nih.gov)
Advances in gene editing such as CRISPR-Cas systems further enhance production efficiency and improve product quality through modification of host cell genomes to manipulate cell growth and apoptosis, and to promote post-translational modification of the gene product. (sigmaaldrich.com)
Usually mutant cells are targeted for apoptosis (programmed cell death) but not always. (emfacts.com)

3 H]Flunitrazepam and [ 3 H]TBOB binding, [ 3 H]GABA uptake, cell viability, and morphology were assayed. (hindawi.com)
We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
Moreover, 1 h exposure to 1 μM YTX was sufficient to inhibit beating activity and to cause irreversible reduction of cardiac cells viability. (units.it)

In vitro, the toxicity was evaluated in human monocyte cells (THP-1) wild-type and NLRP3-deficient cells at a concentration range of 0-100 microg/ml. (cdc.gov)
This increase in toxicity was attenuated in NLRP3-deficient cells. (cdc.gov)
In vitro toxicity studies play an important role in the development of new drugs and therapies. (acmeresearchlabs.in)

Rising demand for biopharmaceutical products including antibodies, vaccines, proteins, and drugs is another major factor driving the growth of the cell culture protein surface coating market. (prnewswire.co.uk)
Self-coating was the largest revenue grossing segment of the cell culture protein surface coatingmarket in 2015. (prnewswire.co.uk)
The long-term health of all metazoan cells is inextricably linked to protein quality control. (uni-bremen.de)
Because cell lines are unique and require different nutrients to thrive, culture biomass and protein production are largely dependent on the culture media used. (sigmaaldrich.com)
The lab has developed paradigm shifting data over the past decade that has led to the conclusion that pre-cancerous metaplasia does not arise from altered resident stem cells in the stomach, but rather develops from transdifferentiation of protein-secreting chief cells into metaplastic mucous-secreting cells. (vumc.org)

Although bacterial, yeast, and insect cell expressions systems are capable of overexpressing recombinant proteins, mammalian cells are most commonly used for biologics production due to their ability to propagate human viruses, express monoclonal antibodies, and incorporate post-translational modifications such as glycosylation that are critical to the production of effective biologics. (sigmaaldrich.com)

Endometrial co-cultures remained viable and showed significant remodeling. (datadryad.org)
Biomimetic endometrial milieus offer new strategies in reproductive techniques and endometrial repair and our findings demonstrate that EndoECM has potential for in vitro endometrial culture and as treatment for endometrial pathologies. (datadryad.org)

Several mammalian cell lines, including CHO, lymphoma (NS0, SP2/0) and human embryonic kidney (HEK) cells, have been developed and used to produce therapeutic proteins and antibodies at high levels. (sigmaaldrich.com)

Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
Cultured cells are excellent hosts for the propagation of many types of viruses. (sigmaaldrich.com)
3) using a range of in vitro techniques (including infection in bat cell culture), examine the pathogenesis of these new viruses, and a pool of available bat viruses which have not yet emerged in humans. (hhs.gov)
We compiled data from ferrets inoculated with an extensive panel of over 50 human and zoonotic IAV (inclusive of swine-origin and high- and low-pathogenicity avian influenza viruses associated with human infection) under a consistent protocol, with all viruses concurrently tested in a human bronchial epithelial cell line (Calu-3). (cdc.gov)
IMPORTANCE Both in vitro and in vivo models are employed for assessing the pandemic potential of novel and emerging influenza A viruses in laboratory settings, but systematic examinations of how well viral titer measurements obtained in vitro align with results from in vivo experimentation are not frequently performed. (cdc.gov)

The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)

100 publications within the field of nanotoxicology and the nanoparticle-cell interaction in vitro . (swansea.ac.uk)

Furthermore, they can connect malignant cells to benign cells, including vascular endothelial cells. (frontiersin.org)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)

These parts include a multitude of cell types that evolve during the course of carcinogenesis and cancer cell progression. (frontiersin.org)
Some cancer cells form highly regulated structures, termed invadopodia, which mediate local, enzymatic degradation of extracellular matrix and facilitate cancer cell invasion and migration during metastatic progression. (bvsalud.org)
Mathematical modeling was used to estimate more generalizable key replication kinetic parameters from raw in vitro viral titers, revealing commonalities between viral infection progression in vivo and in vitro. (cdc.gov)
We show that certain viral titer measurements following infection of a human bronchial epithelial cell line are positively correlated with viral titers in specimens collected from virus-inoculated ferrets and employ mathematical modeling to identify commonalities between viral infection progression between both models. (cdc.gov)

Development of serum-free media (SFM) for cultivation and studies of various parasites and cells, and in vitro identification of positive determinants of culture media and their optimal concentrations for parasites and cell growth. (hindawi.com)

As the relative contribution of in vitro experimentation to these rubrics has not been closely examined, we sought to evaluate to what extent viral titer measurements over the course of in vitro infections are predictive or correlates of nasal wash and tissue measurements for IAV infections in vivo. (cdc.gov)

The adherent cells were labeled by the fluorescent dye Hoechst 33342. (nih.gov)
There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)

The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
The vaccine, which is administered subcutaneously over an 18-month schedule, is derived from sterile, acellular filtrates of microaerophilic cultures of the avirulent, nonencapsulated V770-NP1-R strain of Bacillus anthracis . (cdc.gov)
However, in response to the continuing controversy surrounding this vaccine, the possibility of its being contaminated with mycoplasma was examined by two different techniques. (cdc.gov)

The software allows the automated segmentation of the nuclei in a very dense cell environment, the measurement of the fluorescence intensity of each nucleus and the recording of their position in the plate. (nih.gov)
The cells with a given fluorescence intensity can then be located easily on the recorded image of the culture plate for further analysis. (nih.gov)
This technique is based on fluorescence polarization anisotropy measurements and on the use of Green Fluorescent Proteins [10]. (fresnel.fr)

The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
is the term applied to laboratory techniques using cell-cultures (tissue culture), as distinct from those using human subjects or live animals for testing possible harmful products. (emfacts.com)
The laboratory uses live cell, confocal and SIM imaging to characterize apical vesicle trafficking in whole tissue as well as in cultured enteroids from mouse, pig and human sources. (vumc.org)

Cells with the rare stem-cell like phenotype can be efficiently identified in the undisturbed cultures. (nih.gov)
Increasing focus on stem cell research and development is the most substantial factor that drives the market growth during the forecast period. (prnewswire.co.uk)
Stem cell therapy is the most promising method to treat severe medical conditions such as cardiovascular disease, brain disease, cell deficiency therapy, and blood disease. (prnewswire.co.uk)
Survey Report: Where is Pluripotent Stem Cell Research Now? (stemcell.com)
We are interested in investigating the potential neuroprotective benefits of stem cell transplantation, which has produced encouraging results in different models of CNS degeneration. (nih.gov)
Stem cell technology offers a potential technique to repair the ruptured AF by enabling new matrix synthesis at the defect site. (utwente.nl)
Stem cell infusion is a relatively simple process that is performed at the bedside. (medscape.com)
This is a tool to help students find the right project for their Bachelor's and Master's thesis at Lund Stem Cell Center. (lu.se)

We monitor our cells after transplantation using standard in vivo assessments as listed above. (lu.se)

It often has multiple types of cells mirroring their counterpart in the body along with constituents of native extracellular matrix (ECM) and is typically miniature or micro-scale in size. (stemcell.com)

Cell culture has contributed to the development of vaccines for many diseases. (wikipedia.org)
We invite investigators to contribute original research articles as well as review articles that will motivate the continued exerted efforts in creating an understanding on the In Vitro and Ex Vivo Cultivation and Studies of Parasites and Development of Culture Media and other novel culture and maintenance conditions and applications. (hindawi.com)
Culture medium optimization is another critical component in the development of cell culture processes for the manufacturing of biologics. (sigmaaldrich.com)
1 Today, cell culture has already helped countless discoveries, such as the development of vaccines against poliomyelitis, measles, mumps and other infectious diseases. (integra-biosciences.com)
Due to their opened polymer chain and increased peptide availability to cells, such hydrogels better supported the 3D culture of primary astrocytes, which present high complexity and process branching, allowing the development of improved brain ECM-mimicking systems. (bvsalud.org)

Gene expression analysis suggested that matrix metalloproteinase synthesis was inhibited in cells expanded in 2% oxygen. (ox.ac.uk)

These studies have led to several findings, for example that certain cytokines are generated by beta cells themselves, sometimes under stress, and also that there are probably key factors that render beta cells susceptible to immune attacks. (selectbiosciences.com)
Mechanistically, the importance and meaning of these observations needs to be addressed in a suitable and easily manipulable in vitro system consisting of human islets and immune cells. (selectbiosciences.com)
We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)

The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. (sigmaaldrich.com)

Anatomy9

Organisms6

Diseases3

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes9

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science2

Health Care2

Differentiation14

Experiments13

Proliferation9

Embryos24

Stromal cells6

Tumor Cells2

Embryo11

Methods7

Nanoparticles5

Biology9

20222

Molecular6

Assays4

Approaches5

Fertilization1

Cardiomyocytes2

Microenvironment2

Vivo tissue2

Tissues6

Neurons6

Progenitor cells3

Chondrogenic3

Synthetic2

Organs1

Assay5

Murine2

Fetal1

Mimic3

Epithelial cells3

Microscopy3

Study10

Ross Granvill1

Grown5

Mice1

Oocytes4

Monolayers2

Tissue Engineering3

Involves3

Populations3

Microscopic4

Apoptosis3

Viability4

Toxicity3

Protein5

Commonly1

Endometrial2

Human Embryonic1

Viruses6

Bone marrow and peripheral blood1

Nanotoxicology1

Endothelial2

Progression4

Serum-free1

Predictive1

Adherent cells2

Vaccine4

Fluorescence3

Laboratory3

Stem-cell8

Transplantation1

Typically1

Development5

Oxygen1

Immune4

Viral1