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CryopreservationCryoprotective AgentsSemen PreservationVitrificationFreezingPropylene GlycolFertility PreservationEthylene GlycolDimethyl SulfoxideTissue PreservationSperm MotilitySpermatozoaCell SurvivalFertilization in VitroEmbryo TransferSperm BanksOrgan PreservationReproductive Techniques, AssistedOocytesTissue SurvivalOvaryTissue BanksBlood PreservationSemen AnalysisEmbryo, MammalianBlastocystEmbryo Culture TechniquesSperm RetrievalOvarian FollicleFertilityGlycerolPropylene GlycolsPregnancyPrimary Ovarian InsufficiencySperm Injections, IntracytoplasmicPregnancy RateFetal ViabilityReproductive TechniquesPrenatal InjuriesInfertilityTromethamineOocyte RetrievalSemenIceRaffinoseEmbryo ImplantationEgg YolkSperm CountAcrosomeCell Culture TechniquesTime FactorsMicrobial ViabilityInfertility, FemaleCleavage Stage, OvumDry IceEjaculationBlastomeresPreservation, BiologicalTrehaloseSucrosePregnancy OutcomeOrgan Preservation SolutionsZona PellucidaLive BirthExtreme ColdEmbryonic DevelopmentSpermatogoniaInsemination, ArtificialSpecimen HandlingInfertility, MaleTestisGentianaThermal ConductivityOligospermiaFertilizationOocyte DonationTissue Culture TechniquesOvulation InductionMorulaAntelopesZygoteOsmotic PressureEmbryonic and Fetal DevelopmentCold TemperatureTissue and Organ HarvestingCocosPhascolarctidaeCell Membrane PermeabilityCentrifugationPropidiumTemperatureAquaporin 3Viviparity, NonmammalianApoptosisSingle Embryo TransferCells, CulturedCulture MediaMice, Inbred ICRTeratologyBiological Specimen Banks
EmbryosMotilityEmbryoEffect of cryopreservationVitrificationFertilityOvarian tissueProtocol for cryopreservationAssayOocyte cryopreservationToxicityAnalyzed using flow cytProtocolsMethodsCryoprotectiveMycoplasmaCells viabilityMaintain viabilityIntegrityProcedureMorphologyMononuclear cellsBiologicalLiquid nitrogenMethodologiesAssaysSemenColony ManagementPluripotentOptimalVitroInvestigatorsCellularStrainsStrawsViableReproductiveBlastocyst cultureZhanOrganTemperatureHighFertilizationHumanPrimordialMethodContaminationStem
Embryos10
  • Oocyte cryopreservation can also be part of routine infertility treatments, with efficiency comparable to embryo cryopreservation, [ 41 , 54 ] and may help to overcome legal restrictions, religious concerns or problems with the fate of embryos of divorced couples. (medscape.com)
  • The first and last straw will be subsequently thawed and allowed to mature to the blastocyst stage to demonstrate viability of the line with the assumption that all embryos frozen between the first and the last straw will behave similarly. (unmc.edu)
  • For an additional charge, cryopreserved embryos can be thawed and transferred to foster females to demonstrate viability of the line. (unmc.edu)
  • If homozygous embryos are preserved, we recommend the storage of at least 300 embryos, in addition to those frozen and thawed to ascertain viability. (unmc.edu)
  • Until recently, cryopreservation of two-cell stage or later stage embryos was the predominant method for preserving lines of laboratory rodent models . (taconic.com)
  • For several years, cryopreservation of embryos was the only practical option for securing lines of laboratory rodents. (taconic.com)
  • Another unique application of embryo cryopreservation is during a backcross breeding program, in which embryos from each generation of the backcross can be cryopreserved in the event one wishes to return to a specific generation for further study. (taconic.com)
  • Vitrification as an alternate method of cryopreservation of biological material was first suggested by Luyet 5 , but the practical application of this technology was not fully realized until the successful cryopreservation of mouse embryos by vitrification 2 . (taconic.com)
  • Women can be offered cryopreservation of embryos, oocytes, or ovarian tissue. (biomedcentral.com)
  • Drs. Bischof and Hays recently published a paper describing a simple, optimized approach for the cryopreservation of Drosophila melanogaster embryos and long-term storage in liquid nitrogen (Figure 1). (nih.gov)
Motility6
  • Both cell lines demonstrated increased cell motility and invasive ability after cryopreservation. (researchsquare.com)
  • We will validate successful cryopreservation by motility analysis at no additional cost. (fredhutch.org)
  • Sperm motility does not guarantee viability. (fredhutch.org)
  • Thermal performance to maintain the temperature between + 2°C and + 15°C for 48 hours, cooling kinetics during the first hour of storage, and biological parameters relating to motility, velocity and sperm viability were analyzed and compared to a control box. (researchgate.net)
  • ing to motility , velocity and sperm viability were analyzed and compared to a contr ol box. (researchgate.net)
  • Effect of cryoprotectant on the motility, viability, fertilization, and DNA integrity of naleh fish Barbonymus sp. (bvsalud.org)
Embryo8
  • In some countries including Italy, where embryo cryopreservation was banned, embryologists turned energetically to oocyte cryopreservation, while others have almost entirely neglected this possibility. (medscape.com)
  • It includes IVF techniques such as oocyte/embryo culture, insemination ( including ICSI ), blastocyst culture and transfer, embryo and oocyte cryopreservation (egg freezing) , vitrification, and laser assisted hatching. (sdfertility.com)
  • Nonetheless, there are certain scenarios where embryo cryopreservation offers certain advantages and therefore remains the better option for archiving genetically engineered rodent models. (taconic.com)
  • The main advantage of embryo cryopreservation is that it secures the full, diploid genome of the model. (taconic.com)
  • In essence, embryo cryopreservation represents the "freezing down" of animals which would have otherwise been born and perhaps used in studies. (taconic.com)
  • This advantage of embryo cryopreservation is especially useful when preservation of the genetic state of a genetically modified line on a mixed genetic background is critical. (taconic.com)
  • The two most common methods for embryo cryopreservation are controlled-rate/equilibrium freezing 1 , 3 , 4 and vitrification/non-equilibrium freezing 2 , 3 , 4 . (taconic.com)
  • Also included are two new chapters on predicting embryo developmental potential and viability using automated time-lapse analysis (Eeva) and predicting embryo implantation potential using Embryoscope. (springer.com)
Effect of cryopreservation3
Vitrification4
Fertility4
  • Use of cholesterol-loaded cyclodextrin in donkey semen cryopreservation improves sperm viability but results in low fertility in mares. (bvsalud.org)
  • The success of cryopreservation is dependent upon the fertility of the animals used and the response of the females to superovulatory hormones. (unmc.edu)
  • Additionally, the viability of the produced adults is apparently unaffected by the cryopreservation process, with fertility and offspring quality comparable with those of control mussels. (vliz.be)
  • Dr. Leah Donahue at Jackson Laboratory (under grant 1U42RR033367-01) will generate 166 mouse lines per year from IKMC ES cells, test and analyze mice for viability and fertility, and examine reporter gene expression patterns, while also aiming to reduce the cost of providing mice and resources to the phenotyping centers through the use of innovative technologies. (nih.gov)
Ovarian tissue2
Protocol for cryopreservation2
  • To develop a protocol for cryopreservation and recovery of human endometrial epithelial cells (eECs) retaining molecular and functional characteristics of endometrial epithelium in vivo. (escholarship.org)
  • We have developed a protocol for cryopreservation of eECs in which recovered cells after thawing demonstrate morphologic, transcriptomic, and functional characteristics of human endometrial epithelium in vivo. (escholarship.org)
Assay10
  • Cell viability was examined using a LIVE/DEAD assay which provided fluorescent staining. (ox.ac.uk)
  • A viability assay is an assay that is created to determine the ability of organs, cells or tissues to maintain or recover a state of survival. (wikipedia.org)
  • Tetrazolium reduction: One useful way to locate and measure viability is to complete a Tetrazolium Reduction Assay. (wikipedia.org)
  • The tetrazolium aspect of this assay, which utilizes both positive and negative charges in its formula, promotes the distinction of cell viability in a specimen. (wikipedia.org)
  • Resazurin reduction: Resazurin Reduction Assays perform very closely to that of a tetrazolium assay, except they use the power of redox to fuel their ability to represent cell viability. (wikipedia.org)
  • Sodium-potassium ratio: Another kind of assay practices the examination of the ratio of potassium to sodium in cells to serve as an index of viability. (wikipedia.org)
  • Frogging" is a type of viability assay method that utilizes an agar plate for its environment and consists of plating serial dilutions by pinning them after they have been diluted in liquid. (wikipedia.org)
  • Cryopreservation also influenced the integrity of DNA in the comet assay. (cdc.gov)
  • There is no need for intermediate treatment steps such as spin-and-rinse of cells, viability determinations or cell titer adjustments prior to assay setup. (indigobiosciences.com)
  • Here, we optimized a cell viability assay using plateable cryopreserved human hepatocytes in a 1536-well-plate format. (nih.gov)
Oocyte cryopreservation2
  • Although lately promising signs show increasing application all over the world - for example, according to recent reports, more than half of North American ART clinics offer oocyte cryopreservation [ 50 , 51 ] - it is probably worth summarizing the possible areas and situations where oocyte cryopreservation could be considered as an option or even the only solution. (medscape.com)
  • Thus far, the most exploited application of oocyte cryopreservation is oocyte donation, [ 55 ] where oocyte banks may offer higher safety to prevent disease transmission, less waiting, more choice and more efficiency to use appropriately the valuable biological material. (medscape.com)
Toxicity1
Analyzed using flow cyt1
  • Sperm viability at the end of the 48 hours was analyzed using flow cytometry. (researchgate.net)
Protocols4
  • However, there are a variety of common protocols utilized for further observation of viability using assays. (wikipedia.org)
  • Since then, antimicrobial disinfection protocols have been modified to improve efficacy and valve viability, thereby increasing the supply of usable allografts (1,8). (cdc.gov)
  • 2 Demirci B., Lornage J., Salle B., Frappart L., Franck M., Guerin J.F. Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols. (thieme-connect.de)
  • It is concluded that the addition of synthetic amino acids in the semen of sheep before cryopreservation improves sperm quality and fertilization potential and can thus be added in cryopreservation protocols. (bvsalud.org)
Methods6
  • This funding opportunity announcement (FOA) solicits applications from small business concerns (SBCs) for Small Business Technology Transfer (STTR) projects that propose innovative research in the areas of animal germplasm cryopreservation methods, reagents, equipment, biosecurity, and the detection of disease, including the vertical transmission of disease. (nih.gov)
  • If, for example, cryopreservation method A resulted in 60% viability & 80% structural preservation, whereas method B resulted in 50% viability & 90% structural preservation - the organ cryopreservationist would prefer method A, whereas the cryonicist might prefer method B. Normally, good structure leads to good function, but it is possible that (for example) methods to minimize damage upon rewarming could entail methods that are suboptimal for structural preservation on cooling. (benbest.com)
  • Design your own methods for cryopreservation of biological products, or analytical samples, with high consistency and reproducibility. (smartfreez.com)
  • A broad variety of techniques employed at the CCAP, at UTEX, and elsewhere, can be found in Day, J.G. and Brand, J.J. Cryopreservation Methods for Maintaining Microalgal Cultures, in Algal Culturing Techniques, ed. (utex.org)
  • In this webinar presentation, we will discuss reliable and economic methods for determining cell proliferation and cell viability. (norecopa.no)
  • Dr. Zhan emphasized that although other cryopreservation methods for Drosophila have been published, the team's paper reports a simple yet robust approach for potential wide adoption by other laboratories. (nih.gov)
Cryoprotective2
Mycoplasma1
  • These topics include choices in cell culture media/reagents, cryopreservation factors that affect cell lines and primary cells, and hard-to-detect mycoplasma contamination. (norecopa.no)
Cells viability1
  • Use the Digital Twin (CFD simulation) to understand the impact of your parameters on key stresses for cells viability. (smartfreez.com)
Maintain viability2
  • Various cell types can therefore be effectively preserved, although cell damage during the freezing process must also be minimized in order to maintain viability upon thawing. (labmanager.com)
  • It is critical for cells to freeze at the right temperature to maintain viability, and when scientists are working with temperature-sensitive materials, they need to use the right tools to keep the temperature constant to ensure experimental success. (corning.com)
Integrity5
  • This study examined the influence of the collection and cryopreservation of buccal cells on cell survival and DNA integrity. (cdc.gov)
  • Optimum storage will ensure sample integrity and viability is maintained, while poor storage can cause a material to become compromised, leading to unwanted financial losses and inaccurate results. (corning.com)
  • FM and TEM techniques confirmed that FSH 6cH promoted viability and ultrastructural integrity of follicles after 7 days of culture. (thieme-connect.de)
  • Cryopreservation stabilizes genomic integrity, preserves culture quality, minimizes maintenance costs and reduces the risk of catastrophic loss. (utex.org)
  • The present device meets an unmet need for retrieving a portion of a frozen sample stored in polyvinyl cryopreserved bags, resealing the remainder of the sample and preserving the cryopreserved state and integrity of the rest of the cellular product without compromising viability and sterility. (nih.gov)
Procedure4
  • Cryopreservation of in vivo -matured oocytes has been a demanding task and a frustratingly inefficient procedure for decades, and accordingly this option was not considered as viable in assisted reproduction. (medscape.com)
  • Controlled-rate freezing before long-term sample storage is a vital step in the cryopreservation procedure, since the rate at which cells are cooled has an extremely significant influence on cell survival. (labmanager.com)
  • You will be notified upon the successful completion of our cryopreservation procedure. (unmc.edu)
  • Dr. John Bischof , a professor in the Department of Mechanical and Biomedical Engineering at the University of Minnesota (U of M), explained that the conceptually simple procedure of vitrifying biological samples reflects a complex optimization process to ensure viability. (nih.gov)
Morphology1
  • Endometrial epithelial cells recovered after cryopreservation (n = 5) displayed epithelial morphology and expressed E-cadherin (CDH1), occludin (OCLN), claudin1 (CLDN1), and keratin18 (KRT18). (escholarship.org)
Mononuclear cells2
  • Our renowned scientific team can isolate mononuclear cells with high viability and purity from both peripheral and cord blood samples without any genetic or chemical modifications during the process. (reprocell.com)
  • This document describes procedures for for the isolation and cryopreservation of spleen mononuclear cells (SMNC) using Ficoll method. (nih.gov)
Biological2
  • We ensure you receive ethically sourced, high-quality biological material and ship all our human primary cell products with a Certificate of Analysis (CoA) showing guaranteed quality control testing for cell count, viability, and purity. (stemcell.com)
  • The objective of this study was to establish a protocol for the cultivation of somatic cells present in the feather follicle and to evaluate their viability for the formation of a biological resource bank of wild birds. (usp.br)
Liquid nitrogen1
  • Cryopreservation using liquid nitrogen frozen polyvinyl bags allows for storing cellular materials for extended periods while maintaining their activity and viability. (nih.gov)
Methodologies2
  • Still, the cryopreservation methodologies have remained largely the same. (taconic.com)
  • JRA2 has developed reproducible cryopreservation methodologies for various life-stages of a range of marine macro-organisms and cryo-recalcitrant microorganisms. (vliz.be)
Assays5
  • Viability assays provide a more precise basis for measurement of an organism's level of vitality. (wikipedia.org)
  • Viability assays can lead to more findings than the difference of living versus nonliving. (wikipedia.org)
  • ATP:ATP is a common energy molecule that many researchers hold extensive knowledge of, thus carrying over to how one understands viability assays. (wikipedia.org)
  • As with many kinds of viability assays, quantitative measures of physiological function do not indicate whether damage repair and recovery is possible. (wikipedia.org)
  • Cell viability/proliferation can be measured quickly and easily with a number of assays, including the ATCC MTT and XTT proliferation kits, as well as the new Reliablue™ cell viability regent. (norecopa.no)
Semen1
  • Therefore, we firstly demonstrated that frozen semen treated with CLC in S-MEDIA extender before freezing improves the in vitro sperm viability, but semen treated or not with CLC in S-MEDIUM extender results in a very low conception rate in mares inseminated with thawed donkey semen . (bvsalud.org)
Colony Management1
Pluripotent1
  • No. 5284) available from Tocris, which improves the viability of pluripotent stem cells (PSCs). (tocris.com)
Optimal3
  • Optimal slow cooling conditions are defined by the cooling rate that permits some cell shrinkage without significant amounts of intracellular ice being formed, resulting in maintained cell viability. (labmanager.com)
  • The results signify strong evidence for the suitability of this cryopreservation method for use in mussel aquaculture and in research, where animals must be in optimal health. (vliz.be)
  • In addition, controlled-rate PBMC freezing can be performed in a low-endotoxin, serum-free and/or xeno-free cryopreservation medium to preserve their optimal viability and functionality upon thaw. (reprocell.com)
Vitro1
  • FSH 6cH (24 h) treatment maintained the viability, and promoted the activation and in vitro growth of ovine PFs. (thieme-connect.de)
Investigators3
  • Investigators should contact the cryopreservation service once they have ascertained that they have sufficient numbers (see below) of fertile males and, if needed, female mice. (unmc.edu)
  • The advent of sperm cryopreservation as a popular alternative, in 1992, provided investigators with an additional option for preservation of modified animal models. (taconic.com)
  • Because live Drosophila stocks are costly and labor-intensive to maintain, cryopreservation would provide investigators with new capabilities for research. (nih.gov)
Cellular1
  • Stem Cell Training's two-day intensive "Adipose-derived Stem Cell and Bone Marrow and Platelet Rich Plasma (PRP)" training course covers concepts in cellular medicine, cell viability basics, basic fat and bone marrow stem cell harvesting, isolation and processing procedures, and principles of platelet rich plasma (PRP) processing. (stemcellscourse.com)
Strains4
  • Due to the inherent value of genetically altered mouse strains, we offer cryopreservation of individual mouse strains housed at UNMC. (unmc.edu)
  • Strains that are maintained only in cryopreservation (designated 'cryo only') require a minimum of 4-weeks to grow out before shipment. (utex.org)
  • Dr. Thomas Hays , a U of M professor in the Department of Genetics, Cell Biology, and Development, cited the potential impact of the cryopreservation research effort in managing the large volume of Drosophila strains in laboratories, including national and international stock centers that are used by biomedical researchers around the world. (nih.gov)
  • Certain strains appear to tolerate the cryopreservation process better than others. (nih.gov)
Straws1
  • We collect and cryopreserve 75-100uL of sperm in each of 21 straws with cryopreservation media at two different cassettes in our tightly controlled facility. (fredhutch.org)
Viable1
  • Each unit contains a minimum of 9 × 10 8 total nucleated cells with at least 1.25 × 10 6 viable CD34+ cells at the time of cryopreservation. (nih.gov)
Reproductive1
Blastocyst culture1
Zhan1
  • Dr. Zhan, the paper's lead author, explained that researchers have faced three major challenges for Drosophila cryopreservation. (nih.gov)
Organ1
  • The objectives of organ cryopreservationists is to maximize viability, ie, to maximize living functionality of organs rewarmed from cryopreservation. (benbest.com)
Temperature1
High2
  • Here, we deployed high-throughput screening strategies to identify a small-molecule cocktail that improves viability of hPSCs and their differentiated progeny. (nih.gov)
  • They trained non-specialists, including high school and other students who had no prior training in Drosophila or cryopreservation. (nih.gov)
Fertilization1
Human1
Primordial2
Method2
Contamination1
  • Though this technique is highly accessible, successful propagation of cells for stock expansion or modeling experiments can be plagued by contamination or other conditions that negatively impact cell viability. (sigmaaldrich.com)
Stem2
  • The researchers identified an investigational drug, Emricasan, that, when combined with Chroman 1, could provide additional support to improve stem cell viability. (nih.gov)
  • The researchers showed, for example, that CEPT improved the biobanking of stem cells, called cryopreservation, which involves freezing the cells and typically is very stressful for them. (nih.gov)