- Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µM) to which a chelating group has been immobilized and subsequently charged with Ni 2+ ions. (sigmaaldrich.com)
- Localized mast cell degranulation induced by concanavalin A-sepharose beads. (rupress.org)
- Concanavalin A (Con A) covalently linked to Sepharose 4B beads induced localized degranulation of sensitized rat peritoneal mast cells in regions of contact between beads and cells. (rupress.org)
- This degranulation was Ca2+ dependent and was not seen when sensitized mast cells bound to beads conjugated with a nonstimulating lectin, wheat germ agglutinin, or when unsensitized mast cells bound to Con A-Sepharose. (rupress.org)
- The finding that sensitized mast cells which had adhered to Con A-Sepharose beads degranulated in regions of the cell away from the area of bead contact if exposed to soluble Con A excluded the possibility that the localized release was due to a redistribution of the IgE receptors or putative Ca2+ channels to the region of bead contact. (rupress.org)
- Protein G-Sepharose beads (Cat# M1301) for IgG purification contain covalently coupled recombinant Protein G to 6% cross-linked Sepharose beads, the most popular resin for protein purification. (transcriptionfactor.org)
- Immunoprecipitation of NIH/3T3 cell lysates, untreated or PDGF-treated, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose ® Bead Conjugate) compared to supernatants collected after immunoprecipitation from the same lysate. (cellsignal.jp)
- Sepharose is a tradename for a crosslinked, beaded-form of agarose, a polysaccharide polymer material extracted from seaweed. (wikipedia.org)
- Protein-analysis] Re: Affinity chtomatography - Sepharose vs Sephacryl? (bio.net)
- We harvested recombinant S2 from cell culture supernatants 3 days postinfection and purified it using StrepTactin sepharose affinity chromatography (IBA, https://www.iba-lifesciences.com). (cdc.gov)
- Corneal epithelial cell lysate was harvested and angiostatin was isolated using lysine sepharose. (nih.gov)
- ToxOut™ Protein G (Sepharose) Antibody Purification Kit is a ready-to-use kit for endotoxin-free IgG purification and immunoprecipitation. (transcriptionfactor.org)
- Sepharose is a registered trademark of Cytiva (formerly: GE Healthcare and Pharmacia, Pharmacia LKB Biotechnology, Pharmacia Biotech, Amersham Pharmacia Biotech, and Amersham Biosciences). (wikipedia.org)
- Ni Sepharose High Performance is compatible with all commonly used aqueous buffers, reducing agents, and denaturants such as 6 M Gua-HCl and 8 M urea, as well as a range of other additives, and allows thorough procedures for cleaning the medium ( Characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products ). (sigmaaldrich.com)
- The good flow properties and high resolution make Ni Sepharose High Performance the choice for high-performance purifications for the main characteristics of Ni Sepharose High Performance. (sigmaaldrich.com)
- Ni Sepharose High Performance is supplied preswollen in 20% ethanol, in pack sizes of 25 and 100 mL, as well as in the convenient prepacked formats described later in this chapter. (sigmaaldrich.com)
- Ni Sepharose High Performance precharged with Ni2+ for high-performance purification of histidine-tagged proteins. (sigmaaldrich.com)
- This sample preparation procedure is applicable for all formats containing Ni Sepharose High Performance ( Cell Lysis ). (sigmaaldrich.com)
- Sepharose/agarose, combined with some form of activation chemistry, is also used to immobilize enzymes, antibodies and other proteins and peptides through covalent attachment to the resin. (wikipedia.org)
- Ni-NTA Agarose provides Ni-NTA coupled to a Sepharose CL-6B support and offers high binding capacity and minimal nonspecific binding (see figure One-step purification under native conditions. One-Step Purification under Native Conditions. Human serum response factor (SRF) was expressed in HeLa cells carrying a vaccinia virus vector and purified using Ni-NTA Agarose with different imidazole concentrations in the wash and elution steps. Proteins were visualized by silver staining. CL : cell lysate; FT : flow-through; W1 : 0.8 mM wash; W2 & W3 : 8 mM wash; W4 & W5 : 40 mM wash; E1 & E2 : 80 mM elution. (Data kindly provided by H. Stunnenberg, EMBL, Heidelberg, Germany.) "> One-step purification under native conditions ). (qiagen.com)
- This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems. (sigmaaldrich.com)
- BC: Well those are gels, agarose gels, that can separate proteins based on size, but previously Sephadex could only separate up to 200,000 molecular weight and sepharose could separate up to 1 million molecular weight, and we were interested in IgM immunoglobulin and it was about 1 million molecular weight. (nih.gov)
- Q-sepharose, 20 mM Tris-Cl, pH 8.0, 10 uM EDTA is suitable for most proteins. (uni-konstanz.de)
- Sepharose is a common matrix used to purify proteins, but is usually modified with affinity groups before use. (bitesizebio.com)
- Despite being unmodified, sepharose binds to proteins due to its hydrophilic nature (it is a polysaccharide). (bitesizebio.com)
- We harvested recombinant S2 from cell culture supernatants 3 days postinfection and purified it using StrepTactin sepharose affinity chromatography (IBA, https://www.iba-lifesciences.com). (cdc.gov)
- Brain calmodulin was purified by affinity chromatography on fluphenazine-Sepharose. (erowid.org)
- Such chromatography gels on the market include Blue-Sepharose 6 FF from Cytiva (formerly GE Healthcare). (bioprocessintl.com)
- Elute from the affinity step is loaded onto a polishing column, typically for ion-exchange chromatography (IEC): e.g., cation-exchange media such as carboxymethyl (CM) cellulose and anion-exchange media such as Cytiva Q Sepharose Fast Flow (Q-FF) gel and Bio-Rad Laboratories Macro-Prep High Q media, and diethylaminoethyl (DEAE) cellulose. (bioprocessintl.com)
- Fragmentation patterns of a MUC5AC rich sample mucin were analysed by Sepharose CL-2B gel chromatography. (hindawi.com)
- The catalytic subunit of protein phosphatase 2A (PP2A(c)) was purified from Neurospora crassa extract by (NH4)(2)SO4-ethanol precipitation followed by DEAE-Sephacel, heparin-Sepharose, and MonoQ chromatography steps about 900-fold to a specific activity of 1200 U/g with a 2% yield. (ed.ac.uk)
- Optimal purification of DNA from compost was achieved by chromatography using Sepharose 4B columns. (nih.gov)
- Postconfluent cells synthesize a proteoheparan sulfate of Mr 350,000 (as determined by gel permeation chromatography) which has affinity for transferrin as well as for octyl-Sepharose. (lu.se)
- was isolated by affinity chromatography of muscle cell plasma membranes on laminin-Sepharose. (nih.gov)
- This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from Cytiva. (sigmaaldrich.com)
- Multiple methods have been used, such as biotinylation of RNA and immobilization on streptavidin sepharose. (nih.gov)
- Sepharose is a registered trademark of Cytiva (formerly: GE Healthcare and Pharmacia, Pharmacia LKB Biotechnology, Pharmacia Biotech, Amersham Pharmacia Biotech, and Amersham Biosciences). (wikipedia.org)
- This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva. (sigmaaldrich.com)
- Resin types===== IDA, NTA and other proprietary chelators Specific resins/manufacturers: Ni-NTA (Qiagen), Talon (Clontech), Ni sepharose FF (GE Healthcare/Amersham), Ni MCC (Bioline), His-select (Sigma-Aldrich) Different metal ions =====Elution methods===== *Competitive elution with imidazole or (less commonly histidine), increasing competitor concentration in either a step-wise or gradient manner. (uni-konstanz.de)
- Immunoprecipitation of NIH/3T3 cell lysates, untreated or PDGF-treated, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose ® Bead Conjugate) compared to supernatants collected after immunoprecipitation from the same lysate. (cellsignal.jp)
- A third type of proteoheparan sulfate (Mr 200,000) without affinity for transferrin nor octyl-Sepharose was associated with postconfluent cell layers but not with proliferating ones. (lu.se)
- Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification. (sigmaaldrich.com)
- From serum we were able to get the IgM pure on sepharose columns that nobody had ever had pure. (nih.gov)
- After incubating concentrated GFP with sepharose for less than an hour, I could not remove residual GFP even after dozens of column volumes of buffer and overnight rinsing! (bitesizebio.com)
- The RNA fragments were removed by filtration through a column of Sepharose 4B with a bed volume ten times that of the sample. (nih.gov)
- So we actually did electron microscopy of virus antibody complexes and we could see big, large molecular weight antibodies attached to polioviruses, and then we were - the timing was really kind of amazing - we were in Sweden in Stockholm and Pharmacia, the company, was in Uppsala developing Sephadex sepharose. (nih.gov)