• Aqueous
  • Bright light and heat oxidize the stain, especially when in aqueous solution and will cause precipitation of insoluble precipitates e.g. of methylene violet (Bernthsen). (wikipedia.org)
  • In this method, better ionization during the dilution by the aqueous buffer in this step is necessary to complete the staining. (wikipedia.org)
  • basophilic
  • Plastids in these sections appeared intensely basophilic when stained with azure B. Both the basophilia and radioactivity were removable with ribonuclease, clearly demonstrating the occurrence of RNA in these organelles. (rupress.org)
  • Mature plastids, when stained with azure B, did not appear basophilic under the light microscope. (rupress.org)
  • biological
  • Staining is not limited to biological materials, it can also be used to study the morphology of other materials for example the lamellar structures of semi-crystalline polymers or the domain structures of block copolymers. (wikipedia.org)
  • In vitro staining involves colouring cells or structures that have been removed from their biological context. (wikipedia.org)
  • This means that samples of the manufacturer's batch have been tested by an independent body, the Biological Stain Commission (BSC), and found to meet or exceed certain standards of purity, dye content and performance in staining techniques. (wikipedia.org)
  • dilute
  • To achieve desired effects, the stains are used in very dilute solutions ranging from 1:5000 to 1:500000 (Howey, 2000). (wikipedia.org)
  • 2 Add twice the volume of pH 6.8 buffered water (i.e. if e.g. 7 drops of stain was used, then use 14 drops water) to dilute the stain, taking caution that the stain should not overflow (which will make the dilution inaccurate). (wikipedia.org)
  • smear
  • A common method is: 1 Take an air dried blood smear on a glass slide and cover the smear with the undiluted stain. (wikipedia.org)
  • The stained smear should grossly appear neither too pink nor too blue (verify final results microscopically). (wikipedia.org)
  • tissues
  • however, staining can also reveal where certain chemicals or specific chemical reactions are taking place within cells or tissues. (wikipedia.org)
  • freshly
  • To obtain optimum color reaction, some suggest that 3-5 days should be allowed before using freshly made stain. (wikipedia.org)
  • crimson
  • The Book of the Damned consists of three short novellas set in Paradys, an alternate world version of Paris: Stained with Crimson, Malice in Saffron, and Empires of Azure. (wikipedia.org)
  • Stained With Crimson is the story of Andre St. Jean, a poet in 19th-century Paradys, who is given a ruby scarab ring by a mysterious stranger and falls in love with Antonina, a sinister noblewoman who only comes out at night. (wikipedia.org)
  • cells
  • Those stains excluded by the living cells but taken up by the already dead cells are called vital stains (e.g. trypan blue or propidium iodide for eukaryotic cells). (wikipedia.org)
  • Note that many stains may be used in both living and fixed cells. (wikipedia.org)
  • It stains the trophozoite Trichomonas vaginalis, which presents with greenish discharge and motile cells on wet prep. (wikipedia.org)
  • Its original role was to stain the small cells of keratinizing squamous cell carcinoma present in sputum. (wikipedia.org)
  • Cells with high content of keratin are yellow, glycogen stains yellow as well. (wikipedia.org)
  • Metaplastic cells often stain both green and pink at once. (wikipedia.org)
  • chemicals
  • Both the Methanol bottle and the newly prepared Stain solution should be in containers with appropriate labels and precautions as Flammable and Toxic chemicals (Methanol if ingested can cause blindness and also is dangerous if directly contacts the eye, thus ideally, face shields, gloves, type N95 (US) or type P1 (EN143) respirator filter, are strongly recommended when a large volume of methanol is being used, as during making reagents in bulk. (wikipedia.org)
  • light
  • The stock stain should be kept in a tightly stoppered light opaque (e.g. amber) container in a cool dark place. (wikipedia.org)
  • glass windows
  • One visitor to the synagogue observed, upon entering through a quiet courtyard, viewing the azure stained glass windows and walking across a sand covered floor toward the carved mahogany Holy Ark that the sand floors remind congregants "of how its Jewish ancestors on the Iberian peninsula covered the floors of their makeshift prayer houses so that their footsteps would be muffled and the suspicion of potential denouncers would not be aroused. (wikipedia.org)
  • Two stained glass windows were made by Kempe Studios in 1891 and 1896. (wikipedia.org)
  • either
  • If either of the MycoPerm stains show as a backorder, they may be taken from a kit if stock is available - please ask if unsure. (proscitech.com.au)