Unbound complementary dna strand dna complementary. Medical search. Frequent questions

The sequences of nitrogen bases in a DNA sequence form genes. (homeworkjoy.com)
The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are immobilized on a solid substrate. (tutorialspoint.com)
The principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid molecules. (tutorialspoint.com)
The probes are complementary to specific sequences of DNA or RNA molecules that are to be analysed. (tutorialspoint.com)
The hybridization occurs between the complementary sequences of the probes and the target molecules. (tutorialspoint.com)
The DNA microarray contains thousands of known DNA sequences that are complementary to the DNA of the sample being tested. (tutorialspoint.com)
The DNA from the sample binds to the complementary sequences on the microarray, and the amount of bound DNA is measured to determine the expression level of each gene in the sample. (tutorialspoint.com)
The cDNA microarray uses complementary DNA as probes, while the oligonucleotide microarray uses short DNA sequences as probes. (tutorialspoint.com)
Antibodies tagged with short DNA sequences are a key component for DNA microscopy experiments. (abocdn.com)
A multiplexed array was fabricated by distributing up to 18 different microsphere sensors into the optical fiber array with species-specific 50-mer DNA probes corresponding to 6 BWAs of interest ( Bacillus anthracis , Yersinia pestis , Francisella tularensis , Brucella melitensis , Clostridium botulinum , and vaccinia virus) and 1 BWA simulant, Bacillus thuringiensis kurstaki , with each organism represented by at least 2 probe sequences ( Table A1 ). (cdc.gov)
The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences to address a wide ranging biological problem in food industry, diagnostic laboratories and forensic medicine. (hindawi.com)
Molecular biologists describing DNA sequences or referring to one of the two strands of double- stranded DNA frequently use complementary pairs of terms, such as coding/ non- coding, sense/ nonsense or transcribing/ non- transcribing. (genomicglossaries.com)
When DNA sequences are described by giving the sequence of only one strand, this is usually the strand with the same sequence as the RNA (messenger, ribosomal, transfer, etc.) and should therefore be called the coding strand. (genomicglossaries.com)
Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
c , Using orthogonal DNA sequences (blue and green) and sequential acquisition as in Exchange-PAINT, localizations from targets spaced more closely than the SMLM resolution limit can be unambiguously assigned for each target. (nature.com)
During the cooling phase of a thermal annealing ramp, the staple strands anneal to the scaffold strands by complementary base pairing, with faster rates being directly related to the affinity strength of their complementary sequences. (ubcbiomod.com)
Antisense oligonucleotides (ASOs), defined as short synthetic oligonucleotides with single-stranded sequences complementary to certain mRNA sites, have been under drug development for approximately 30 years, with the first FDA-approved drug, Fomivirsen, being approved in 1998 ( Stein and Castanotto, 2017 ). (aspetjournals.org)
Nucleic acid-based aptamers were first described in 1990, where the first aptamers were in vitro selected using a random library of single-stranded oligonucleotides sequences [ 1 ] by a selection procedure known as systematic evolution of ligands by exponential enrichment (SELEX). (encyclopedia.pub)
SELEX starts by chemical synthesis of a library of random sequences of double-stranded DNA (dsDNA). (encyclopedia.pub)
While DSBs involve in binding to particular dsDNA sequences, to modulate the process of transcription, to cleave DNA molecules, or to be involved in chromosome packaging and transcription in the cell nucleus, etc. (biomedcentral.com)
Chromatin insulators are DNA sequences that function in the epigenetic regulation of gene expression, including three-dimensional structural alterations of the chromatin. (biologists.com)

Applications include separation of biotin-labeled biomolecules such as biotin- labeled antibodies, genomic DNA, RNA, PCR products, oligonucleotides (e.g. biotinylated oligo (dT) or peptides. (vectorlabs.com)
Aptamers are short single-stranded RNA or DNA oligonucleotides capable of folding into complex 3D structures, enabling them to bind to a large variety of targets ranging from small ions to an entire organism. (encyclopedia.pub)
Aptamers are single-stranded RNA or DNA oligonucleotides that fold up into a distinctive 3D structure capable of binding with high affinity and specificity to small molecules up to entire organisms, with nanomolar range dissociation constants. (encyclopedia.pub)

The RNA or ribonucleic acid turns genetic information from DNA into proteins. (homeworkjoy.com)
The histones proteins help the DNA to pack into the cells. (homeworkjoy.com)
In Neisseria gonorrhoeae , the primosome proteins that catalyze DNA replication restart differ from the well-studied primosome proteins of E. coli with respect to the number of proteins involved and the affinities of their physical interactions: the PriA:PriB interaction is weak in E. coli , but strong in N. gonorrhoeae , and the PriB:DNA interaction is strong in E. coli , but weak in N. gonorrhoeae . (biomedcentral.com)
Catalyzed by primosome proteins such as PriA, PriB, PriC, DnaT, and DnaG, DNA replication restart pathways facilitate origin-independent reloading of the replicative helicase onto a repaired DNA replication fork in a process that involves coordinated protein and nucleic acid binding within a nucleoprotein complex called the DNA replication restart primosome [ 2 ]. (biomedcentral.com)
PriA is thought to recognize and bind to repaired DNA replication forks and D-loop recombination intermediates, facilitate assembly of the primosome complex by recruiting other primosome proteins, and catalyze duplex DNA unwinding using energy furnished by hydrolysis of ATP [ 4 , 5 ]. (biomedcentral.com)
http://www.somalogic.com/Technology.aspx) offers generated hundreds of single-stranded DNA aptamers (seeing that known seeing that SOMAmer) for more than 1,100 proteins goals that cover a diverse place of molecular features, including receptors, kinases, development elements, and human hormones. (scienceexhibitions.org)
The viral genome is represented by a single-stranded positive-sense RNA, approximately 30 thousand base pairs (bp) long, with 14 open reading frames encoding 27 proteins [2]. (crie.ru)
More specifically, Chemicals describes atoms and complete chemical compounds, Polymer-Segments describe regions within polymers such as proteins and DNA, and Organisms describes the biological organism modeled within a PGDB. (ecocyc.org)
This can be compared to the effects of the histone proteins, H1, H2A, H3 where no changes in the mechanical properties of DNA were observed and the complex of the H3/H4 proteins where a smaller increase in the stiffness is observed (0.2 N m −1 ). (nature.com)
Moreover, as the word 'coding' refers to the relationship between nucleic acids and proteins, rather than the mere transcription of DNA into RNA, it is logical to call the strand with the mRNA sequence the coding strand, as in the first example. (genomicglossaries.com)
However, some scientists think that before life adopted DNA and proteins, it relied primarily on RNA. (elifesciences.org)
Yet, some RNA strands can also fold to form ribozymes, 3D structures that could have guided life's chemical processes the way proteins do now. (elifesciences.org)
All life on Earth today uses a triplet RNA code to make proteins from DNA, and these experiments showed how RNA triplets might have helped RNA sustain early life forms. (elifesciences.org)
Basic ParABS systems comprise two proteins, ParA and ParB, and a centromere-like, cis-acting DNA element called parS . (biorxiv.org)
Historically, ƒ u determination for small-molecule drug candidates has been determined by ultrafiltration, ultracentrifugation, or equilibrium dialysis, which are techniques based on the physical separation of unbound drug molecules from those bound to proteins ( Pacifici and Viani, 1992 ). (aspetjournals.org)
For the characterization of exosomes, micro vesicles (MV) and virions, resumed as bio-nanoparticles (BNPs), fluorescent staining techniques allow fast and specific staining of membranes, surface proteins or nucleic acids by applying lipid dyes, antibodies, and intercalating RNA/DNA dyes [1, 2]. (trillium.de)
DNA binding proteins can be classified into double-stranded DNA binding proteins (DSBs) and single-stranded DNA binding proteins (SSBs), and they take part in different biological functions. (biomedcentral.com)
Experimental results on 10-fold cross validation indicate that the new feature set are effective to describe DNA binding proteins. (biomedcentral.com)
The evaluation results on both bound (DNA-bound) and non-bound (DNA-free) proteins have shown the satisfactory performance of our method. (biomedcentral.com)
Accordingly, the DNA binding proteins usually consist of single-stranded DNA-binding proteins (SSBs) and double-stranded DNA-binding proteins (DSBs). (biomedcentral.com)

Flow-FISH (fluorescence in-situ hybridization) is a cytogenetic technique to quantify the copy number of RNA or specific repetitive elements in genomic DNA of whole cell populations via the combination of flow cytometry with cytogenetic fluorescent in situ hybridization staining protocols. (wikipedia.org)
The third step in DNA microarray analysis is hybridization. (tutorialspoint.com)
The principle of DNA microarray is based on the hybridization of DNA strands. (tutorialspoint.com)
NanoLINK streptavidin magnetic beads are also ideal for generating single-stranded PCR templates (by removal of the unbiotinylated competing PCR strand), which allows for a dramatic increase in the efficiency of hybridization to complementary targets. (vectorlabs.com)
RNA double-strand hybridization is a key player in gene expression regulation. (bvsalud.org)

DNA molecules are very long that they can't fit in cells. (homeworkjoy.com)
The DNA or RNA molecules that are labelled with fluorescent dyes are hybridized to the probes on the microarray chip. (tutorialspoint.com)
The microarray chip is washed to remove unbound or non-specifically bound molecules. (tutorialspoint.com)
Researchers in Germany have developed a DNA-based 'crane' that can pick up molecules from a depot and deposit them precisely at a target site. (chemistryworld.com)
The team designed the geometrical arrangement of the molecules on the chip so that the crane pulled the carrier away from the support strand one base at a time - 'unzipping' the DNA. (chemistryworld.com)

They fixed a short DNA 'hook' to the AFM tip, designed to form a double helix when it binds to a complementary 'carrier' strand. (chemistryworld.com)
When the crane swings around to pick up the cargo, the DNA hook binds firmly to the carrier DNA and pulls it away from the support strand. (chemistryworld.com)
After the crane arrives in the target area, the 'free' end of the carrier DNA binds to the target DNA - again forming a double helix. (chemistryworld.com)
This time, a larger part of the carrier DNA binds to the target strand and when the crane moves away, the carrier and hook separates - leaving the carrier stuck to the target. (chemistryworld.com)
Complementary strategies aimed at prolonging the half-life and reducing the renal clearance of GLP-1 include the development of GLP-1-based analogs such as liraglutide, a DPP-4-resistant fatty-acylated GLP-1 molecule that binds noncovalently to serum albumin and exhibits more potent and sustained glucose-lowering effects compared with native GLP-1 ( 6 ). (diabetesjournals.org)
SSB binds with ssDNA with high affinity and low specificity, and is mainly involved in DNA replication, recombination and repair. (biomedcentral.com)

The function of DnaT is not well understood, but it has been proposed that DnaT binding leads to dissociation of single-stranded DNA (ssDNA) from PriB through a competition mechanism, possibly exposing the ssDNA on the lagging strand template for reloading the replicative helicase, which ultimately leads to fork reactivation [ 8 ]. (biomedcentral.com)
The dsDNA library is either used to synthesize a single-stranded DNA (ssDNA) library or undergoes in vitro transcription to produce an ssRNA library. (encyclopedia.pub)
There are two kinds of DNAs, single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). (biomedcentral.com)

The number of bonds that hold nucleotides from complementary strands depends on the type of nitrogenous base. (homeworkjoy.com)
The nucleotides joined together to form two long strands in the spiral to create a double helix structure. (homeworkjoy.com)
adenine (A) - A nitrogenous base that occurs in DNA and RNA nucleotides and pairs with thymine (in DNA) or uracil (in RNA) through two hydrogen bonds. (rcsb.org)
Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base pairs with complementary messenger RNA. (bvsalud.org)
Modified aptamers may show improved chemical diversity relative to aptamers composed entirely of natural DNA or RNA nucleotides and expand their applications in diagnostics, therapeutics, and nanotechnology [ 1 ] . (encyclopedia.pub)
Length of DNA is based on the number of nucleotides present in it. (bankofbiology.com)

The DNA sequence is so long, so how does the nucleus manage to store DNA and other essential components for cell functioning? (homeworkjoy.com)
It consists of a small glass slide or silicon chip with thousands of small spots, each containing a different DNA sequence that represents a single gene. (tutorialspoint.com)
The biosensor probe identified a target DNA sequence that was several-folds shorter than a typical PCR-template. (hindawi.com)
With the recent and better knowledge on how gene regulation occurs and after intense debate in the academic community 1 , epigenetics has been commonly defined as "the study of changes in gene function that are mitotically and/or meiotically heritable and that do not entail a change in DNA sequence" 2 . (nature.com)
The sense strand has the same sequence as the mRNA transcript. (genomicglossaries.com)
Using three-letter 'triplet' building blocks, this new ribozyme can copy various folded RNA strands, including the active part of its own sequence. (elifesciences.org)
ParABS partition systems, comprising the centromere-like DNA sequence parS, the parS -binding ParB-CTPase and the nucleoid-binding ParA-ATPase, ensure faithful segregation of bacterial chromosomes and low-copy-number plasmids. (biorxiv.org)

These are mRNA and DNA vaccines. (personalizemymedicine.com)
The antisense strand is the template for mRNA synthesis. (genomicglossaries.com)

There are two main types of DNA microarrays: cDNA microarrays and oligonucleotide microarrays. (tutorialspoint.com)
The two main types of DNA microarray are the cDNA microarray and the oligonucleotide microarray. (tutorialspoint.com)
To ensure sensitive detection of BWAs with high accuracy, the performance of this multiplexed, high-density array was assessed by examining the detection limits and specificity of probes with Cy3-labeled synthetic targets (the synthetic target is a 50-mer oligonucleotide complementary to the probe). (cdc.gov)

Bacterial DNA replication restart pathways facilitate reinitiation of DNA replication following disruptive encounters of a replisome with DNA damage, thereby allowing complete and faithful duplication of the genome. (biomedcentral.com)
Consequently, DNA replication forks that stall or collapse due to encounters of the replisome with DNA damage must be reactivated to allow complete replication of the genome and ensure survival of the cell. (biomedcentral.com)
Coronavirus (CoV) is an enveloped virus with a positive single-stranded RNA genome and is pathogenically ranging from 60 to 140 nm in diameter with spike-like projections on its surfaces giving it a crown-like appearance under the electron microscope [ 1 , 2 ]. (springeropen.com)
The papillomavirus E1 helicase is essential for viral DNA replication and plays a key role in controlling viral genome copy number. (biomedcentral.com)

Here is described, how the dissociation constant of RNA double strands can be determined by applying fluorescence correlation spectroscopy. (bvsalud.org)

A DNA (A-form DNA) - A conformation of right-handed, double stranded DNA in which the bases are tilted with reference to the helix axis. (rcsb.org)
This conformation has more base pairs per turn compared to the cannonical B-form DNA. (rcsb.org)
The new conformation brings amino acid groups essential to the enzyme reaction, which in the unbound conformation may distant from the active site, into the active site. (ncl.ac.uk)

Using genomic DNA as common reference in microarray experiments has recently been tested by different laboratories. (biomedcentral.com)
Pair-wise comparison of three experimental conditions was obtained either with two labeled cDNA samples co-hybridized to the same array, or by employing Shewanella genomic DNA as a standard reference. (biomedcentral.com)
This work could serve as a guideline for microarray data analysis using genomic DNA as a standard reference. (biomedcentral.com)
Threats to genomic integrity occur at the ends of every linear chromosome, including incomplete DNA synthesis by the replisome and the potential for inappropriate DNA break repair. (elifesciences.org)

The histones neutralize and bind the negatively charged DNA. (homeworkjoy.com)
The role of adding the MgCl 2 salt is to stabilize the negatively charged DNA backbone (1), which is known to have negatively charged repulsion (2). (ubcbiomod.com)

In humans and other eukaryotic organisms, DNA is contained within structures called chromosomes. (elifesciences.org)
An enzyme called telomerase adds structures called telomeres to the ends of the chromosomes, which protect the DNA from damage. (elifesciences.org)
For example, these triplets work together to bind tightly to RNA strands and unravel structures that block RNA copying. (elifesciences.org)
The self-assembly of DNA origami structures follows a process of denaturation and cooling at respective varying rates. (ubcbiomod.com)

In this article, we will discuss the principle, types, and steps involved in the DNA microarray technology. (tutorialspoint.com)

DNA replication restart pathways provide bacterial cells with a mechanism to reactivate replisomes that are disrupted in this manner [ 2 ]. (biomedcentral.com)
DNA replication restart initiated by PriA helicase is a highly coordinated process and probably represents the major pathway of DNA replication restart in E. coli . (biomedcentral.com)
Triplet substrates thus resolve a central incongruity of RNA replication, and here allow the ribozyme to synthesise its own catalytic subunit '+' and '-' strands in segments and assemble them into a new active ribozyme. (elifesciences.org)
Papillomaviruses undergo a complex life cycle requiring regulated DNA replication. (biomedcentral.com)
This E1 phosphate map will provide a new tool to more fully understand viral replication and serve as a useful model for investigating regulation of viral and cellular DNA replication. (biomedcentral.com)
DSBs usually act as transcriptional factors to regulate the genes' expressions, while SSBs usually play roles in DNA replication, recombination, and repair, etc. (biomedcentral.com)

Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize. (genomicglossaries.com)

and imaging and tracking systems, biosensor systems, and biomarker finding.7,16,17,18 Moreover, the nature of nucleic acid provides another bonus that the function of aptamers can be 176644-21-6 IC50 modulated for desired therapy and drug delivery applications using the complementary oligo antidote strategy. (scienceexhibitions.org)
Recently, improvements in the development of DNA and RNA aptamers that specifically target cell surface receptors have motivated scientists to use cell-specific aptamers that are internalized as targeting ligands for targeted disease therapy. (scienceexhibitions.org)
The SELEX methodology is quite similar for both DNA and RNA aptamers. (encyclopedia.pub)

DNA, known as Deoxyribonucleic Acid, is a complex molecule that stores all the information which is necessary to maintain and build an organism. (homeworkjoy.com)
Viruses come in a variety of shapes and forms and are classified according to a range of different properties, including the type of genetic information that they contain, which can be composed of ribonucleic acid (RNA) or deoxyribonucleic acid (DNA). (personalizemymedicine.com)

The technology involves the preparation of a microarray chip, which consists of a solid substrate, usually glass or silicon, with thousands or millions of immobilized DNA probes. (tutorialspoint.com)
The microarray chip is prepared by depositing DNA probes onto a solid substrate, usually a glass slide or silicon wafer. (tutorialspoint.com)
This illustrates the Lock and Key mechanisms and how the shape of the substrate is exactly complementary to the shape of the active site. (ncl.ac.uk)

All living things contain DNA, and nearly every cell in multicellular organisms includes the full set of DNA. (homeworkjoy.com)
In other words, we can say that, whenever organisms reproduce, the DNA has passed along to the offspring. (homeworkjoy.com)
The DNA allows slight changes in organisms to maintain the diversity of life. (homeworkjoy.com)
Overall, this work defines human telomerase DNA binding and synthesis properties at single-molecule level and establishes conserved telomerase subunit architecture from single-celled organisms to humans. (elifesciences.org)
DNA is the genetic material in most of the organisms. (bankofbiology.com)
Various DNA insulators have been identified or are postulated in many organisms, and the paradigmatic CTCF-dependent insulators are perhaps the best understood and most widespread in function. (biologists.com)

Flow-FISH is most commonly used to quantify the length of telomeres, which are stretches of repetitious DNA (hexameric TTAGGG repeats) at the distal ends of chromosomes in human white blood cells, and a semi-automated method for doing so was published in Nature Protocols. (wikipedia.org)

Surprisingly, telomerase reconstitutions yielded heterogeneous DNA-bound TERT monomer and dimer complexes in relative amounts that varied with assembly and purification method. (elifesciences.org)
We studied CTP- and parS F -modulated ParA F -ParB F complex assembly, in which DNA-bound ParA F -ATP dimers are activated for ATP hydrolysis by interacting with two ParB F N-terminal domains. (biorxiv.org)

The nitrogen bases are vital for the DNA because they store the genetic materials. (homeworkjoy.com)
DNA microarrays are powerful tools for analysing gene expression patterns, detecting DNA mutations, and identifying genetic variations. (tutorialspoint.com)
The data obtained from the microarray chip are analysed using various software tools to identify differentially expressed genes, detect DNA mutations, and identify genetic variations. (tutorialspoint.com)
Like DNA, strands of RNA contain genetic data. (elifesciences.org)
Nucleic acids (DNA & RNA) are the building blocks of genetic material. (bankofbiology.com)

The bases on one strand pairs with the bases on another strand. (homeworkjoy.com)
The fact is that the cells contain approximately 6 billion pairs of DNA and about 2 meters of DNA present in per diploid cell! (homeworkjoy.com)
Length of DNA = number of base pairs X distance between two adjacent base pairs. (bankofbiology.com)

To evaluate probe specificity, arrays containing beads with only a single probe type were exposed sequentially to the synthetic complementary target as well as to all noncomplementary targets. (cdc.gov)

The DNA has almost 3 billion bases. (homeworkjoy.com)
Furthermore, we experimentally resolve the DNA backbone distance of single bases in DNA origami with Ångström resolution. (nature.com)

Several studies implicated that longer DNA targets are broken down to small fragments during extensive food processing causing failure of PCR-based identification methods [ 23 , 26 , 28 , 31 ]. (hindawi.com)

The charge of the DNA is negative because of the phosphate backbone. (homeworkjoy.com)

The different subclasses of Polymer-Segments include different types of DNA sites such as transcription start sites and terminators, and longer regions such as genes. (ecocyc.org)
that the terminology for the strands of DNA with respect to transcription should therefore be formalized and the use of sense strand for DNA be avoided in future literature. (genomicglossaries.com)
One strand of DNA acts as the template for transcription and the other does not. (genomicglossaries.com)
We analyse transcriptional bursting within a stochastic non-equilibrium model which accounts for the coupling between the dynamics of DNA supercoiling and gene transcription. (arxiv-vanity.com)
Here we consider a stochastic model which couples the dynamics of supercoiling and transcription in DNA (first introduced in Ref. (arxiv-vanity.com)

The DNA has four kinds of nitrogenous base: Thymine (T), Cytosine (C), Adenine (A), and Guanine (G). Thymine and Cytosine are pyrimidine and Adenine, and Guanine is purines. (homeworkjoy.com)
It includes Cytosine (C), Thymine (T-only in DNA) & Uracil (U-only in RNA). (bankofbiology.com)

Ultrafiltration, equilibrium dialysis, and ultracentrifugation were assessed for the separation of unbound ASO from biological matrices. (aspetjournals.org)
Protein-DNA interactions are essential for many biological processes. (biomedcentral.com)

At the start, the carrier strand is attached to a so-called DNA 'chip' or array and forms a double helix with another 'support' strand attached firmly to the chip. (chemistryworld.com)
which is the extent of over or under-winding of the two strands in a DNA double helix. (arxiv-vanity.com)

antibodies-online offers a selection of high-quality antibodies suitable for spatial profiling in multiplex IHC, multiplex IF, DNA microscopy or spatial CUT&Tag experiments. (abocdn.com)

We developed a platform to simultaneously detect BWAs with an array composed of DNA probe-functionalized microspheres that are randomly distributed onto microwells generated on the end of an etched coherent optical fiber bundle ( 11 , 12 ) that contains 6,000-50,000 individual fibers. (cdc.gov)
We modified our previous protocol of microsensor preparation ( 12 ) by using an increased concentration of cyanuric chloride solution in the probe activation step to achieve a more complete and efficient coupling of DNA probes to microspheres. (cdc.gov)

This enzyme, which is the unique molecular target of the natural anticancer compound camptothecin, acts by nicking one DNA strand and forming a transient protein-DNA covalent complex. (mdpi.com)

Many scientists are still confused by the terms sense and antisense when referring to DNA because the terminology has changed over the years. (genomicglossaries.com)
When referring to DNA, the terms should be "transcribed strand" and 'non- transcribed strand" The term antisense would be best reserved for RNA. (genomicglossaries.com)

Negative control experiments confirmed that these mechanical and physical effects were induced neither by the acidity of SC4 nor through nonspecific interactions of SC4 on DNA. (nature.com)
Magnetic-tweezers experiments showed that CTP promotes multiple ParB F loading onto parS F -containing DNA, generating condensed partition complex-like assemblies. (biorxiv.org)

The DNA was first figured out by James Watson, Francis Crick, and Maurice Wilkins in 1953. (homeworkjoy.com)
In 1962, Watson, Crick, and Wilkins achieved a Nobel Prize in Medicine for describing the structure of DNA. (homeworkjoy.com)

The diversity of DNA insulators is, however, understudied, especially in the context of embryonic development, when many new gene territories undergo transitions in functionality. (biologists.com)

This communication describes the most current progress of NTA: quantification of colocalization ratios of two complementary fluorescently labelled compartments present on BNPs. (trillium.de)

The sample can be RNA, DNA, or protein, depending on the purpose of the analysis. (tutorialspoint.com)
and results in the formation of a large nucleo-protein complex (the partition complex) around the parS site on the DNA to be partitioned. (biorxiv.org)
Understanding the binding specificity of a DNA binding protein is helpful for the research of protein functions. (biomedcentral.com)
Therefore, a computational method is required to annotate the DNA binding protein as DSB or SSB automatically. (biomedcentral.com)

A DNA microarray is a tool used to measure the expression levels of thousands of genes simultaneously. (tutorialspoint.com)

Some inhibitors have been already approved by FDA, such as Azacitidine and Decitabine as DNA methyltransferase inhibitors for the treatment of myelodysplastic syndrome 8 and Vorinostat and Romidepsin as inhibitors of histone deacetylase for the treatment of peripheral T-cell lymphoma 9 . (nature.com)

In this study, chromatin immunoprecipitation and massively parallel DNA sequencing (ChIP-seq) was performed to identify the AmtR regulon in C. glutamicum ATCC 14067. (frontiersin.org)
By means of Silicon Nano Tweezers (SNTs) the effects on the mechanical properties of λ-phage DNA during interaction with calf thymus nucleosome to form an artificial chromatin analog were measured. (nature.com)

We report that N. gonorrhoeae PriA's DNA binding and unwinding activities are similar to those of E. coli PriA, and N. gonorrhoeae PriA's helicase activity is stimulated by its cognate PriB, as it is in E. coli . (biomedcentral.com)
This finding is significant because N. gonorrhoeae PriB's single-stranded DNA binding activity is weak relative to that of E. coli PriB, and in E. coli , PriB's single-stranded DNA binding activity is important for PriB stimulation of PriA helicase. (biomedcentral.com)
Furthermore, a N. gonorrhoeae PriB variant defective for binding single-stranded DNA can stimulate PriA's helicase activity, suggesting that DNA binding by PriB might not be important for PriB stimulation of PriA helicase in N. gonorrhoeae . (biomedcentral.com)
While it remains unclear if N. gonorrhoeae PriB's weak DNA binding activity is required for PriB stimulation of PriA helicase, the ability of PriB to stimulate PriA-catalyzed ATP hydrolysis could play an important role. (biomedcentral.com)
Recruitment of PriB to a PriA:DNA complex stabilizes PriA on the DNA [ 6 ] and enhances its helicase activity through a mechanism that involves PriB's single-stranded DNA-binding activity [ 7 ]. (biomedcentral.com)

This handbook contains all the information required to take full advantage of Arrayit's Pathway Focused DNA Microarrays. (arrayit.com)
Arrayit uses the highest quality control (QC) and quality assurance (QA) measures to ensure the quality of our ArrayIt® Pathways Human DNA Microarrays. (arrayit.com)
Arrayit Pathways™ Focused Microarrays represent the most advanced human DNA microarrays in the industry. (arrayit.com)

However, all of these methods involve PCR amplification of target DNA that is typically more than hundred base-pair long [ 25 - 30 ]. (hindawi.com)

In their experiment, the researchers used the 'carrier' DNA to ferry a fluorescent tag to the target site, but other kinds of payload could also be attached, they say. (chemistryworld.com)
A) Individual nanosized objects are picked up from discrete storage sites with a DNA strand at the tip of an AFM cantilever and transferred to a target site (B). The length and binding geometry of the strands are chosen so that a hierarchy of unbinding forces allows the repetition of this process over and over again. (chemistryworld.com)
Using the kinetic curve, a convenient method for quantifying and counting target DNA copy number was developed. (hindawi.com)
Drug unbound fraction (ƒ u ) characterization is a key consideration in pharmacokinetic and pharmacodynamic (PK/PD) modeling, assuming only unbound drug can interact with the target, and therefore has direct implications in the efficacy and potential toxicity of the drug. (aspetjournals.org)

show that even complexes with a single TERT are able to add telomeres to DNA. (elifesciences.org)

lt;span lang="EN-US">This review presents the basic principles of application of the loop-mediated isothermal amplification (LAMP) reaction for the rapid diagnosis of coronavirus infection caused by SARS-CoV-2. (crie.ru)

Anatomy9

Organisms18

Diseases1

Chemicals and Drugs102

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes68

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science4

Health Care2

Sequences21

Oligonucleotides3

Proteins20

Hybridization5

Molecules5

Binds6

SsDNA3

Nucleotides6

Sequence7

MRNA2

Oligonucleotide3

Genome4

Dissociation1

Conformation3

Genomic4

Negatively charged2

Structures4

Microarray technology1

Replication6

DNAs1

Aptamers3

Deoxyribonucleic Acid2

Substrate3

Organisms6

Repeats1

Bound2

Genetic5

Pairs3

Synthetic1

Bases2

Targets1

Backbone1

Transcription5

Cytosine2

Biological2

Double2

Antibodies1

Probe2

Enzyme1

Antisense2

Experiments2

Crick2

Gene1

Quantification1

Protein4

Genes1

Histone1

Chromatin2

Helicase5

Microarrays3

Base1

Target4

Telomeres1

Amplification1