Ubiquitin zebrafish proteins. Medical search. Frequent questions

The small 8-kilodalton protein ubiquitin, which marks proteins for degradation, also has features of a heat shock protein. (wikipedia.org)
Treatment with SE486-11 and SAHA improved MYCN ubiquitination and degradation, and inhibited tumorigenesis in neuroblastoma xenografts markedly, and, transgenic mice and zebrafish. (aplarcongress.org)
The phosphatidyl inositol 3-kinase (PI3K)/Akt pathway was involved in this ubiquitin-proteasome-mediated degradation of PDCD4. (plos.org)
Pulsatile shear stress induces ubiquitin-proteasome-mediated degradation of PDCD4 via a PI3K/Akt pathway in HUVECs. (plos.org)
RNF12 mediated ubiquitylation of REX1 leads to REX1 protein degradation. (biologists.com)
Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome. (ucsc.edu)
Both tankyrase isoforms interact with a highly conserved domain of axin and stimulate its degradation through the ubiquitin-proteasome pathway. (plasignaling.com)
However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). (novusbio.com)

Reduced synaptic transmission correlates with increased levels of Ras homolog gene family, member A (RhoA), a KCTD13/CUL3 ubiquitin ligase substrate, and is reversed by RhoA inhibition, suggesting increased RhoA as an important mechanism. (ox.ac.uk)
Among those 13 different types of gene mutation that causes FALS, mutation in Transactive response DNA binding Protein 43kDa (TDP-43) gene is seen in approximately 4% of FALS and 2% of SALS (7). (fromemuseum.org)
Transactive response DNA binding protein 43kDa (TDP-43) is a DNA/RNA-binding protein encode by the TARDBP gene on chromosome 1. (fromemuseum.org)
We demonstrated that the silencing of the ambra1b gene determines a reduction of primordial germ cells (PGCs), a condition that, in the zebrafish, leads to the development of all-male progeny. (biomedcentral.com)
Since data that can be obtained by knockdown approaches are limited to the first developmental stages, we recently generated a mutant zebrafish line for each paralogous gene. (biomedcentral.com)
Mutants, which have a stop codon upstream of the Rabbit polyclonal to CREB.This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins.This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. (aplarcongress.org)
Specifically, the PDCD4 protein combines directly with the mRNA coding region of the target gene ( MYB/c-MYB ) to block translation [2] . (plos.org)
Three mammalian SRPKs - SRPK1, SRPK2, and SRPK3 - relay information between environmental cues and gene expression by regulating SR protein phosphorylation 1,2 . (biologists.com)
Defects in the gene encoding Peroxin 3 are the cause of peroxisome biogenesis disorder complementation group 12 (PBD-CG12) and Zellweger syndrome (ZwS), both of which arise from a failure of peroxisomal protein import. (thermofisher.com)
High density arrays of extraordinarily sensitive integrated microring resonators will allow many gene and protein signatures to be simultaneously quantitated from a single patient sample. (nih.gov)
This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. (thermofisher.com)
Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (thermofisher.com)
This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. (avivasysbio.com)
In addition to Tdp1, they identified a second gene previously identified as TTRAP (TRAF and TNF receptor-associated protein) [13] . (prolekarniky.cz)
Thus, it has evolved a protection mechanism against heat stress by increasing the expression of the gene coding for heat shock protein (HSP) 70 under elevated temperatures. (biomedcentral.com)

Keap1 is a cysteine-rich protein, known to be anchored to actin cytoskeleton [ 5 ], serving as an adaptor protein for the Cul3-dependent E3 ubiquitin ligase complex. (springer.com)
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. (ucsc.edu)
The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. (ucsc.edu)
Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL21 and RBX1. (ucsc.edu)

Results from zebrafish embryos injected with murine Stat3 mRNA and stat3 morpholino suggest that Ambra1b could indirectly regulate this protein through CUL4-DDB1 interaction. (biomedcentral.com)
Dorsal view of 8 dpf zebrafish embryos with head toward the top of the image. (xenbase.org)

He carried out his final year project with Dr. Doryen Bubeck and investigated protein complexes formed by pore-forming proteins. (newsteadgroup.org)
The diversity of processes leading to trapping of topoisomerases suggested that cells might have specific mechanisms to repair the protein/DNA covalent complexes. (prolekarniky.cz)

Further experiments showed that myofibril assembly can be blocked at the nascent myofibril by five different inhibitors of the Ubiquitin Proteasome System (UPS) stage in both avian and human cardiomyocytes. (syr.edu)

The structures of Tdp2 from C. elegans , zebrafish, and mouse have been determined, indicating an active site that accommodates adducted single-strand DNA [16] , [17] . (prolekarniky.cz)

In contrast to a previous knockdown study, deletion of Kctd13 or kctd13 does not increase brain size or neurogenesis in mice or zebrafish, respectively. (ox.ac.uk)

Increased synthesis of selected proteins in Drosophila cells following stresses such as heat shock was first reported in 1974. (wikipedia.org)

We have shown that Myosin1D controls motile cilia orientation in the zebrafish left-right organizer, identifying this actin-binding motor protein as a conserved regulator of animal left-right asymmetry (Juan et al, 2018). (unice.fr)
The cilia are composed of three main sections, the axoneme which performs the sensory or movement function, the transition zone which likely contains over 100 proteins which function to anchor the cilia and regulate transport to and from the cilia , and the basal body which is a centriole that functions as a tubulin organizing center to form the cilia . (xenbase.org)
FIGURE 2 Confocal images of wholemount zebrafish (3dpf) and Xenopus laevis (Stage 37) kidney cilia . (xenbase.org)
FIGURE 4 Confocal images of the motile cilia lining the zebrafish nasal (olfactory) pit. (xenbase.org)
Some studies have suggested a role for this protein in maintaining centriole-nucleated sensory organelles termed primary cilia, and AS is now considered to belong to the growing class of human genetic disorders linked to ciliary dysfunction (ciliopathies). (springer.com)

at the University of Wuerzberg, Germany, where he worked on Ubiquitin-Activating Enzyme Fragment-Based Screening. (newsteadgroup.org)

Although mammalian models provide vital insights into the underlying pathology of these diseases, aquatic organisms such as Xenopus and zebrafish provide valuable tools to help screen and dissect out the underlying causes of these diseases. (xenbase.org)
The zebrafish and Xenopus kidney are outlined in white dashed lines, and motile multiciliated cells in the kidney are pseudocolored in magenta. (xenbase.org)

AMBRA1 is an intrinsically disordered protein, working as a scaffold molecule to coordinate, by protein-protein interaction, many cellular processes, including autophagy, mitophagy, apoptosis and cell cycle progression. (biomedcentral.com)
Elucidate the structure, dynamics and functions of intrinsically disordered proteins and protein regions (IDPs/IDRs) and their biological regulation by Post-translational modifications. (upstate.edu)

protein ubiquitination. (ucsc.edu)

The zebrafish genome contains two ambra1 paralogous genes ( a and b ), both involved in development and expressed at high levels in the gonads. (biomedcentral.com)
Characterization of the zebrafish paralogous genes mutant lines generated by CRISPR/Cas9 approach showed that ambra1b knockout leads to an all-male population. (biomedcentral.com)
By exploiting ambra1a and ambra1b knockout zebrafish lines, we prove the sub-functionalization between the two paralogous zebrafish genes and uncover a novel function of Ambra1 in the protection from excessive PGC loss, which seems to require binding with the CUL4-DDB1 complex. (biomedcentral.com)
Importantly, the absence of AtNOL12 alters the expression of many ribosomal protein and ribosome biogenesis genes. (bvsalud.org)
The multifunctional regulator nuclear factor erythroid 2-related factor (Nrf2) is considered not only as a cytoprotective factor regulating the expression of genes coding for anti-oxidant, anti-inflammatory and detoxifying proteins, but it is also a powerful modulator of species longevity. (springer.com)
The major characteristics of Nrf2 are to some extent mimicked by Nrf2-dependent genes and their proteins including heme oxygenase-1 (HO-1), which besides removing toxic heme, produces biliverdin, iron ions and carbon monoxide. (springer.com)

The Ubiquitin Proteasome System is required for progression from nascent myofibrils to mature myofibrils. (syr.edu)

Thus, our study provides new mechanistic insights into the regulation of axin protein homeostasis and presents new avenues for targeted Wnt pathway therapies. (plasignaling.com)
In fact, Wnt signallingitselfregulatesthelevelofaxinatseveralsteps,with AXIN2 being a major transcriptional target of the b-catenin-T-cell factor (TCF) com- plexandWntsignallingpromotingthedegradationofaxin12,13.However, the molecular mechanisms that regulate protein homeostasis of destruc- tion complex components and complex assembly remain elusive. (plasignaling.com)

Several heat shock proteins function as intra-cellular chaperones for other proteins. (wikipedia.org)
Oxidative stress generated during such stressful conditions may damage DNA and proteins, and as a consequence the cellular processes are disturbed. (springer.com)
We recently described the structure of M. musculus RNF213 (PDB: 6TAX) and characterized its enzymatic activity, revealing it as a large monomeric protein with a functional dynein-like AAA (ATPases Associated with diverse cellular Activities) core that exists alongside a novel E3 ligase module ( 16 ). (biorxiv.org)
Limb-girdle muscular dystrophy (LGMD) protein defects occur in several pathways involved in the biologic function of muscle and can be divided into groups based on cellular localization. (medscape.com)

In 1974, Tissieres, Mitchell and Tracy discovered that heat-shock induces the production of a small number of proteins and inhibits the production of most others. (wikipedia.org)
Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. (avivasysbio.com)

Spinal muscular atrophy (SMA) is a neurodegenerative disease produced by low levels of Survival Motor Neuron (SMN) protein that affects alpha motoneurons in the spinal cord. (mdpi.com)

Its analogy to proteins uL30m and mL63 in mammalian mitochondrial ribosomes also suggests a plausible evolutionary pathway for expansion of protein content in mammalian mitochondrial ribosomes. (bvsalud.org)
Thus, it is likely that axin protein levels need to be tightly regulated to ensure proper Wnt pathway signalling. (plasignaling.com)
Our study uncovers a new mechanism that controls axin protein stability and Wnt pathway signalling, and its therapeutic exploitation holds promise for treating Wnt-pathway- dependent cancers. (plasignaling.com)

This discovery eventually led to the identification of the heat-shock proteins (HSP) or stress proteins whose expression this puffing represented. (wikipedia.org)
Immunoprecipitation revealed that pulsatile shear stress induced the coupling of ubiquitin with PDCD4 expression. (plos.org)
Further examination revealed that PPI hindered the expression of GRP78 protein, resulting in a suppressive effect on SR-HCC cell migration and invasion. (bvsalud.org)
We observed miR-137 expression and activity in sensory neurons including Rohon-Beard neurons and dorsal root ganglia, two neuronal cell types that confer touch-sensitivity in normal zebrafish, suggesting a role of these cell types in the observed phenotype. (nature.com)

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels comprise a small subfamily of proteins within the superfamily of pore-loop cation channels. (cipsm.de)

We found that the human cardiomyocytes developed patterns of protein organization identical to the three-step series seen in the model organisms cited above. (syr.edu)
Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
RNF213/ALO17/mysterin (Ring Finger Protein 213) is the largest known human single-chain E3 ubiquitin ligase, first described as the major susceptibility factor for Moyamoya disease (MMD) ( 1 ), a disorder which causes aberrant vasculature development commonly leading to stroke and other complications ( 2 ). (biorxiv.org)
Zebrafish Ciliopathy Screen Plus Human Mutational Analysis Identifies C21orf59 and CCDC65 Defects as Causing Primary Ciliary Dyskinesia. (xenbase.org)
The N-terminal region of centrosomal protein 290 (CEP290) restores vision in a zebrafish model of human blindness. (xenbase.org)

Heat shock proteins have been found in all species examined, from bacteria to humans, suggesting that they evolved very early and have an important function. (wikipedia.org)

SR protein kinases go beyond splicing. (biologists.com)
Of note, phosphorylation of SR proteins mediated by Serine/arginine protein kinases (SRPKs) drive their functionality 1 . (biologists.com)

These ions also act as cofactors or structural components of proteins or enzymes that regulate several physiological processes such as mitochondrial respiration, energy metabolism, and antioxidants [ 3 , 4 ]. (ijbs.com)

The identification of GTPBP8 as an important player in these processes provides new insights into the molecular mechanisms underlying mitochondrial protein synthesis and its regulation. (bvsalud.org)

The E3 ubiquitin ligase mindbomb1 controls planar cell polarity-dependent convergent extension movements during zebrafish gastrulation. (unice.fr)

Protein synthesis and lipogenesis are highly stimulated in the liver at sexual maturity in hens (15 to 20 fold) to support the incorporation of 0.75 kg proteins and 1.5 kg of triglycerides into the yolk over a year of commercial production. (biomedcentral.com)

Serine/arginine-rich proteins (SR proteins), a group of proteins that harbor an arginine/serine (RS) domain either at their N' or C' terminus, play crucial roles in a plethora of biological processes such as cell cycle and signaling, developmental pathways, DNA replication and repair, transcription and mRNA splicing. (biologists.com)

In this study, we discovered a crucial role for GTP-binding protein 8 (GTPBP8) in the assembly of the mitoribosomal large subunit (mt-LSU) and mitochondrial translation. (bvsalud.org)
Schwer and E. HDM201 mitochondrial protein acetylation is markedly elevated in Sirt3? (aplarcongress.org)
Cuproptosis causes proteotoxic stress and ultimately results in cell death by the binding of Cu ions to lipid-acylated proteins during the tricarboxylic acid cycle of mitochondrial respiration. (ijbs.com)

A key finding from the original identification of Tdp2 was that the protein was more active in processing 5′ phosphotyrosyl-linked oligonucleotides, and that siRNA knockdown of Tdp2 in mammalian cells resulted in sensitivity to etoposide, a drug targeting topoisomerase II, but not camptothecin, a drug that targets topoisomerase I. Recent work has greatly enhanced our understanding of the biochemistry and structural biology of Tdp2. (prolekarniky.cz)

The arrangement of proteins during myofibrillogenesis follows a three-step process: beginning with premyofibrils, followed by nascent myofibrils, and concluding with mature myofibrils (reviewed in Sanger et al. (syr.edu)
HSP70/HSPA1A promotes the proper folding of nascent polypeptides and assists in the refolding of denatured proteins (2). (novusbio.com)

It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)

Heat shock proteins often function as chaperons in the refolding of proteins damaged by heat stress. (wikipedia.org)
To address the mechanism underlying epileptogenesis, we used zebrafish to characterize Pk1a function and epilepsy-related mutant forms. (biologists.com)
This molecule is highly conserved in vertebrates enabling the investigation of its function in the developing zebrafish. (nature.com)
The zebrafish ( Danio rerio ) is an attractive and versatile model to gain insight into vertebrate brain development and function. (nature.com)
These include proteins associated with the sarcolemma (see image below), proteins associated with the contractile apparatus (see image below), and various enzymes involved in muscle function. (medscape.com)
Specific protein function and abnormalities are discussed below with each LGMD. (medscape.com)

ALMS1 encodes a ~ 0.5 megadalton protein that localises to the base of centrioles. (springer.com)

A conserved protein binding domain of approximately 80 amino-acid alpha crystallins are known as small heat shock proteins (sHSP). (wikipedia.org)
Peroxin 3, also known as PEX3 (peroxisomal biogenesis factor 3) or TRG18, is a 373 amino acid multi-pass membrane protein that localizes to peroxisomes and belongs to the Peroxin family. (thermofisher.com)
Heat Shock 70kDa Protein (HSP70), also known as Heat Shock 70kDa Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70 kDa. (novusbio.com)

Heat shock proteins (HSP) are a family of proteins produced by cells in response to exposure to stressful conditions. (wikipedia.org)

Apart from MMD, RNF213 has been implicated in other vascular pathologies like intracranial atherosclerosis ( 3 ) and in angiogenesis, as shown by studies in mice ( 4 ), zebrafish ( 5 ), and tissue culture ( 4 , 6 ). (biorxiv.org)

Mark joined the Newstead lab in 2019 as a Clarendon Scholar undertaking his DPhil research on lysosomal membrane proteins. (newsteadgroup.org)

Gabriel is currently the manager of our tissue culture suite and researchers novel methods for eukaryotic membrane protein production. (newsteadgroup.org)

During heat stress, outer membrane proteins (OMPs) do not fold and cannot insert correctly into the outer membrane. (wikipedia.org)

Zebrafish studies have shown that Pk1 coordinates cell movement, neuronal migration and axonal outgrowth during embryonic development. (biologists.com)

hspb1 (HSP27) is expressed during stress and during the development of embryo, somites, mid-hindbrain, heart and lens in zebrafish. (wikipedia.org)
Egg yolk constitutes a major source of proteins, minerals, vitamins and lipids for the developing embryo. (biomedcentral.com)

En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
The authors identified E3 ubiquitin ligase RNF12 (also known as RLIM) as a potential SRPK substrate using an in silico motif search analysis in the mouse proteome database. (biologists.com)

Heat-shock proteins are named according to their molecular weight. (wikipedia.org)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)

However, some studies suggest that an increase in damaged or abnormal proteins brings HSPs into action. (wikipedia.org)
However, in the mid 1990s, other proteins were also found to accumulate in the abnormal muscle fibers, and molecular genetic studies revealed several chromosomal loci. (medscape.com)

These results raise the question of the biological significance of egg yolk proteases and antiproteases, and more generally of all minor proteins that have been identified in egg yolk. (biomedcentral.com)

Some bacterial heat shock proteins are upregulated via a mechanism involving RNA thermometers such as the FourU thermometer, ROSE element and the Hsp90 cis-regulatory element. (wikipedia.org)
Jonathan completed his DPhil at the University of Oxford in the Kleanthous group researching bacterial protein antibiotics that specifically target multidrug resistant P. aeruginosa . (newsteadgroup.org)

As a consequence, the heat shock proteins are also referred to as stress proteins and their upregulation is sometimes described more generally as part of the stress response. (wikipedia.org)

These include 37 proteases and antiproteases, which are likely to play a role in the formation of the yolk (vitellogenesis), as regulators of protein metabolism. (biomedcentral.com)

Anatomy44

Organisms9

Diseases2

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Psychiatry and Psychology1

Phenomena and Processes60

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Degradation8

Gene15

Ligase complex4

Embryos2

Complexes2

Inhibitors1

Elegans1

Neurogenesis1

Drosophila1

Cilia5

Enzyme1

Xenopus2

Intrinsically disordered2

Ubiquitination1

Genes6

Proteasome1

Homeostasis2

Cellular4

Inhibits2

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Pathway3

Expression4

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Human5

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Kinases2

Regulate1

Mechanisms1

Planar1

Synthesis1

MRNA1

Mitochondrial3

Mammalian1

Nascent2

Subunit1

Function6

Encodes1

Amino-acid3

Stressful conditions1

Mice1

20191

Tissue1

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Neuronal1

Embryo2

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