Types lymphocyte culture test mixed. Medical search. Frequent questions

Technically, it is an ex-vivo cellular immune assay that occurs between two allogeneic lymphocyte populations (same species but genetically distinct). (wikipedia.org)
The assay set-up consists of purifying responder lymphocytes from peripheral blood, thymus, lymph nodes or spleen and co-culturing with stimulator cells. (wikipedia.org)
Further characterization of the lymphocytes, accessory cells (dendritic cells, macrophage) and cytokines that participate in the MLR have been done as this assay continues to be used to define mechanisms for understanding cellular immune function in vitro. (wikipedia.org)
Therefore, DNA testing cannot be used in place of enzyme assay to establish the diagnosis. (medscape.com)
Additionally, MarinBio specializes in in vitro cell-based assay testing services for clinical and pre-clinical studies, including method development, qualification, validation, transfer, lot-release testing, and potency testing according to guidelines from both FDA and International Council for Harmonization (ICH). (marinbio.com)
The mixed lymphocyte reaction (MLR) assay was used to evaluate the function of DCs. (frontiersin.org)
Flowcytometry and enzyme-linked immunosorbent assay (ELISA) tests were employed to evaluate subpopulation of T-lymphocytes and the levels of cytokines, respectively. (biomedcentral.com)

MZB was found to inhibit both humoral and cellular immunity by selectively inhibiting lymphocyte proliferation, which led to its development as an immunosuppressive agent. (hindawi.com)

Put simply, it is mixing populations of T-lymphocytes (category of white blood cells) together, and measuring the reaction that occurs. (wikipedia.org)
T cells are a type of white blood cell that scans for cellular abnormalities and infections. (wikipedia.org)
After several days, lymphocytes underwent blast transformation, DNA synthesis and cellular proliferation in response to the major histocompatibility antigen (MHC Class I and II) differences between the two cell populations designated as Responder and Stimulator cells. (wikipedia.org)
No MLR occurred when the Responder cells came from thymectomized animals, whereas bursectomized chicken leukocytes reacted in culture demonstrating that T-cells were the major cell type in Responder cell populations. (wikipedia.org)
Stimulator cell populations that also contain T-cells (Two way mixed lymphocyte reaction) will replicate in the presence of the Responder cells, therefore for a One way mixed lymphocyte reaction, stimulator cells are prevented from replicating by irradiation or treatment with mitomycin C, a DNA crosslinker to prevent cell replication. (wikipedia.org)
After a second centrifugation step, HBSS was removed and cells were resuspended in 5 mL culture medium and incubated for another 20 - 22 h (24 h fixation time). (europa.eu)
In six patients and six controls allogenic mixed leucocyte reaction (AMLR) was performed using mDC and cord blood-derived naive T cells at a DC/T naive ratio of 1 : 200. (torvergata.it)
Moreover, proliferative response of allogenic naive T cells cultured with mDC was impaired in the patients (13471 +/- 9917.2 versus 40976 +/- 24527.2 cpm, P = 0.04). (torvergata.it)
Our proposal focuses on the possibility to test agents selectively reducing the fraction of GPI-AP deficient cells and consequently improving the growth of GPI-AP normal cells. (aamds.org)
Our proposal is based on the possibility to pre-clinically test drugs improving the function of normal cells and decreasing the fractions of cells with PIGA mutations. (aamds.org)
Using a large screening of chemicals and natural compounds already approved by the U.S. Food and Drug Administration or tested in previous studies we identified one chemical substance called CGS- 15943 which has an effect in improving the proliferation of normal cells by decreasing the number of PNH cells. (aamds.org)
Periodically 'cleansed' of spontaneous mutants: Prior to dose-range finding and mutagenicity testing, the mouse lymphoma cells were grown for 1 day in R10-medium containing hypoxanthine, aminopterine and thymidine to reduce the amount of spontaneous mutants, followed by a recovery period of 2 days on R10-medium containing hypoxanthine and thymidine only. (europa.eu)
Cells were exposed to the test item in basic medium supplemented with 5%/10% (v/v) heat-inactivated horse serum (R5-medium) for 3/24 hour exposure. (europa.eu)
CTLL-2 cells were cultured with increasing concentrations of client drug for four days. (marinbio.com)
MarinBio's contract research and development service professionals are expert in designing customized or standardized bioassays and in the measurements of interactions of drugs (including small molecules, biologics, or device interactions) with cultured cells and primary cells. (marinbio.com)
MarinBio has extensive experience with cells and cell banks at both non-GMP/‑GLP and GMP/GLP levels of many tissue cell types and clones, in addition to custom development of cells, clones, or sub-clones. (marinbio.com)
Adipose tissue-derived MSCs had greater proliferative potential than bone marrow-derived MSCs, while no significantly difference in colony efficiency were observed between the two types of cells. (biomedcentral.com)
However, bone marrow-derived MSCs possessed higher capacity toward osteogenic and chondrogenic differentiation compared with adipose tissue-derived MSCs, while similar adipogenic differentiation potential wase observed between the two types of cells. (biomedcentral.com)
There is experimental evidence from in vitro studies of animal cells that some cannabinoids, including THC, can produce a variety of changes in cellular processes in vitro (i.e. in the test tube). (druglibrary.org)
Synergy among thymocytes and peripheral lymph node cells in the in vitro generation of lymphocytes cytotoxic to alloantigens. (ox.ac.uk)
Besides CD19 + CD138 − B cells, plasmablasts, and plasma cells were enriched in the tongue of mice colonized with C. albicans suggesting a potential role of B lymphocytes during oral fungal colonization. (frontiersin.org)
T cell preparation: Patients and donors undergo apheresis to obtain peripheral blood mononuclear cells (PBMCs), which are expanded in culture. (knowcancer.com)
A novel retrovirus, human T -celllymphomaleukemia virus (HTLV), has been isolated from the fresh and cultured cells of two of these patients. (science-connections.com)
Subsequent characterization of this virus has shown that it is not significantly related to any known animal retrovirus, is not an endogenous (genetically transmitted) virus of man, and so far has been associated only with fresh or cultured T -cells from patients with T -cell neoplasia. (science-connections.com)
1975) have required the establishment of continuously growing cell lines from the disease of origin or cocultivation of these cells with previously established cell lines, the ability to grow malignant T -cells in long-term culture could facilitate the isolation of retroviruses from these diseased states. (science-connections.com)
The types of T-cells, their function and their interactions with other cells to elicit, sometimes degrading, but at other times helpful effects, particularly as an immunotherapy, are complex and difficult to analyze. (preferred-cell-systems.com)
For example, although mixed lymphocyte reactions (MLR) are used to better define donor and patient histocompatability, mixed lymphocyte cultures (MLC) can be used to study specific T-cells and their functions and interactions, such as agents that modulate APC-mediated T-cell activation. (preferred-cell-systems.com)
Finally, we investigated the impact of natural CD25(+) Treg cells on the production of T-helper (Th)1 and Th2 cytokines in mixed lymphocyte cultures between C57BL/6 CD4(+) CD25(-) T cells as responders and bm12 or (C57BL/6X bm12)F1 antigen-presenting cells as stimulators. (ox.ac.uk)
Finally, natural CD25(+) Treg cells inhibited the production of interleukin (IL)-2, interferon-gamma, IL-5, and IL-13 in mixed lymphocyte culture in a dose-dependent manner. (ox.ac.uk)
CONCLUSION: Natural CD25(+) Treg cells control Th1- and Th2-type allohelper T-cell responses and thereby influence the fate of allografts in nonimmunosuppressed recipients. (ox.ac.uk)
Quickly, 20,000 cells in 0.5% agar (Bacto-Agar, Difco Laboratories) were split on preformed 0.8% agar level using a 35 mm Petri pots and pans (Non tissues growing culture, Fisher Scientific). (mindunwindart.com)
The number displays that these making it through cells could still type mammospheres. (mindunwindart.com)
Additionally, we observe that tumors which relapse after treatment display a significant increase in the numbers of PDL1 capable cells present suggesting that specific editing of mixed tumors might play a role in disease relapse. (bmj.com)
While PDL1 can be found on a variety of cell types within the tumor microenvironment [ 5 - 10 ], expression is most frequently observed on malignant tumor cells themselves were it can range from completely PDL1 − to virtually 100% PDL1 + [ 11 - 15 ]. (bmj.com)
The first, inducible expression, presents as a spatially separated PDL1 − core surrounded by PDL1 + cells and results from upregulation of PDL1 by IFNγ expressed from tumor infiltrating lymphocytes (TIL) [ 17 , 18 ]. (bmj.com)
Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. (thermofisher.com)
Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. (thermofisher.com)
Our results show that CNCM I-3690 can (i) physically maintain modulated goblet cells and the mucus layer and (ii) counteract changes in local and systemic lymphocytes. (nature.com)
Diagnosis is by detecting lymphopenia, absence or a very low number of T cells, and impaired lymphocyte proliferative responses to mitogens. (msdmanuals.com)

1980. "Lymphokine Enhances the Expression and Synthesis of Ia Antigens on Cultured Mouse Peritoneal Macrophages. (rockefeller.edu)

1992. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: An alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. (wikipedia.org)
Lymphocytes (0.4 mL blood of a healthy male donor was added to 5 mL or 4.8 mL culture medium, without and with metabolic activation respectively and 0.1 mL (9 mg/mL) Phytohaemagglutinin) were cultured for 48 h and thereafter exposed to selected doses of test substance for 3 h, 24 h and 48 h in the absence of S9-mix or for 3 h in the presence of S9-mix. (europa.eu)
Five patients had progressive disease, two patients had stable disease, and one patient experienced a partial response after receiving donor lymphocyte infusions and IFN-α. (aacrjournals.org)
Patients' PBMCs are irradiated and mixed with donor PBMCs. (knowcancer.com)

The bone marrow- and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). (biomedcentral.com)
MZB has a very specific mechanism of action on the lymphocytes that inhibits their proliferation without interfering with purine synthesis in other cell types. (hindawi.com)
We showed higher DTH, increased lymphocyte proliferation, decreased tumor growth and reduced JAK2/STAT3 phosphorylation in mice treated with naringenin and CPT. (biomedcentral.com)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

They respond to IL-25, IL-33 and TSLP and produce both amphiregulin, which is involved in tissue repair, and classical type-2 cytokines including IL-4, IL-5 and IL-13. (elifesciences.org)
Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)

Results from this experiment were confirmed by those of a second test with groups of female mice of the same strain fed dietary levels of captan ranging from 500 to 16 000 ppm for up to 213 days. (inchem.org)

mDC, derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) and stimulated with lipopolysaccharide (LPS) for the last 24 h, were phenotyped for the expression of the co-stimulatory molecules B7.1 and B7.2. (torvergata.it)
PBMCs include lymphocytes, monocytes, and DCs. (frontiersin.org)

Why are Mixed Lymphocyte Reactions Performed? (wikipedia.org)
The purified BDC elicited strong allogeneic mixed leukocyte reactions and HLA classes II- and I-restricted antigen-specific primary immune responses. (nih.gov)
Other test results usually are noncontributory, unless systemic reactions are noted. (medscape.com)
Intracutaneous tests with species-specific extracts: These can detect immediate and delayed-type reactions. (medscape.com)

Definitive diagnosis requires the measurement of acid alpha-glucosidase activity in cultured skin fibroblasts or peripheral blood lymphocytes. (medscape.com)
Skin biopsy findings reveal acid alpha-glucosidase activity in cultured fibroblasts. (medscape.com)
In order to reach the Giesma dye phase, the researchers had to take human lymphocytes and fibroblasts that were going through mitosis and then expose them to colchicine. (psychesystems.com)

There is, at most, mixed evidence that THC and other cannabinoids are mutagenic in standard microbial assays, such as the Ames test, and there is contradictory evidence on whether the cannabinoids are clastogenic, i.e. produce breaks in chromosomes. (druglibrary.org)

Quality Assurance also includes testing for sterility, pH, osmolority, presence, or absence of Mycoplasma, endotoxin and presence of Human Leukocyte Antigen (HLA Ag) by Complement Dependent Cellular Cytotoxicity (CDCC) and flow cytometry. (labquip.net)
This material was tested and found to be non-reactive for hepatitis B Surface Antigen (HBsAg), Anti-HIV and Hepatitis C. (labquip.net)
It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs. (lookformedical.com)
In children who have completed immunizations with diphtheria, pertussis, and tetanus (DPT) or Hib-conjugated vaccines, the antibody response to protein antigens can be tested in adults and older children by measuring IgG antibodies to tetanus or diphtheria toxoid and H influenzae type b (Hib) polysaccharide antigen. (medscape.com)
Hepatitis B is not a reliable antigen for testing immune competence because of the high frequency of nonresponders in the population, particularly in persons older than 40 years. (medscape.com)
Immunotherapy engages the immune system in the battle against cancer by taking advantage of the capacity of the T lymphocyte for antigen-directed cytotoxicity [ 14 ]. (biomedcentral.com)

A mixed leukocyte analysis may lead to errors because granulocytes also contain a renal isomer of acid maltase, which is active in an acidic pH. (medscape.com)
In the other 18 subjects, the responses to PHA and PWM were significantly reduced along with that of mixed leukocyte cultures. (cdc.gov)
LK-induced Ia-bearing Mphi were tested as primary mixed leukocyte reaction stimulators. (rockefeller.edu)

Pooled Human Serum is also used as Negative control in Elisa and in serological HLA Typing. (labquip.net)
Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. (lookformedical.com)

Serologic tests: These may reveal elevated immunoglobulins directed against specific antigens in patients with delayed cutaneous manifestations. (medscape.com)
The antigens tested included bovine, porcine, ovine and equine osteochondral samples that have been treated in successive steps of photooxidation. (biomedcentral.com)

Under the study conditions, the test substance is not considered to be mutagenic in the presence and absence of exogenous metabolic activation. (europa.eu)
OECD 471 with 1,3 -BDDMA, GLP, +/- metabolic activation: negative with all strains tested. (europa.eu)
Experiment I (Plate incorporation): Test solution at each dose level (solvent or reference mutagen solution (positive control)), S9 mix (for test with metabolic activation) or S9 mix substitution buffer (for test without metabolic activation), bacteria suspension and overlay were mixed in a test tube and poured onto the selective agar plates. (europa.eu)
Experiment II (Pre-incubation): The test solution at each dose level, the S9 mix (for test with metabolic activation) or S9 mix substitution buffer (for test without metabolic activation) and the bacteria suspension were mixed in the test tube and incubated at 37 +/- 1.5°C for 60 minutes. (europa.eu)

Eight concentrations were tested for toxicity and mutation induction with each 3 plates. (europa.eu)
Serum PBB concentrations, however, were not correlated with lymphocyte function. (cdc.gov)

Studies of lymphocyte mitogenic function in 45 exposed Michigan dairy farm ers showed normal responses to phytohemagglutinin (PHA) and pokeweed mitogen (PWM) and a reduced reactivity in mixed lymphocyte cultures in 27 subjects. (cdc.gov)

1964. Lymphocyte interaction: A potential histocompatibility test in vitro. (wikipedia.org)

Finally, SpaFED pili are clearly important effectors since an L. rhamnosus ΔspaF mutant failed to provide the same benefits as the wild type strain. (nature.com)

PHS supports Mixed Lymphocyte Culture (MLC) and Mitogen (PHA) Response. (labquip.net)

Findings of the scalp biopsy, including the type of inflammation present, location and amount of inflammation, and other changes in the scalp, are necessary to diagnose the type of cicatricial alopecia, to determine the degree of activity, and to select appropriate therapy. (wikipedia.org)
Clinicopathologic findings suggested a moderate, predominantly macrophagic, mixed inflammatory process. (cdc.gov)
This review summarizes the published findings on the efficacy of MZB for renal disease including IgA nephropathy, lupus nephritis, and NS, as well as of oral MZB pulse therapy for severe lupus nephritis and NS, and also the mechanism of the effect of oral MZB pulse therapy on the lymphocyte cell cycle. (hindawi.com)

This study aims to conduct a direct head-to-head comparison of the biological characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability to be used in regenerative medicine applications. (biomedcentral.com)
Under human platelet lysate-supplemented culture conditions, bone marrow- and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. (biomedcentral.com)

Third, if cannabinoids have immunosuppressive effects, then this would have mixed implications for their therapeutic use. (druglibrary.org)

Translocations in peripheral blood lymphocytes were scored by using fluorescence in situ hybridisation whole-chromosome painting. (cambridge.org)
The identification of individual types of chromosomes and translocations or deletions allows the opportunity to diagnose specific diseases with precision. (psychesystems.com)

All patients achieved initial mixed hematopoietic chimerism with two patients rejecting their graft and recovering host hematopoiesis. (aacrjournals.org)
lncRNAs and mRNAs in the NP group (DCs isolated from NP) and the test group (DCs isolated from patients with AR) were identified via chip technology and bioinformatic analyses. (frontiersin.org)
Clinicians should not refer patients to CDC to find out where or how to get tested for COVID-19 or how to get COVID-19 test results. (cdc.gov)

The types of samples studied are usually samples that come from blood, tissue, or bone marrow. (psychesystems.com)

If cannabis use affects cellular processes then users may be at increased risk of developing various types of cancer, and if it affects the immune system then cannabis users may be at increased risk of contracting infectious diseases and developing cancer. (druglibrary.org)
Use or application goal (research, cellular therapy or toxicity testing). (preferred-cell-systems.com)

While many mixed breed dogs have a significantly lower degree of inbreeding, many populations such as local stray cat populations may actually have a higher than expected degree of inbreeding. (vin.com)
However, dominant disease and polygenetic diseases may be just as likely to be seen in mixed breed populations as they are in more inbred populations depending upon the disease and the population. (vin.com)

The MLR was first recognized when researchers mixed leukocytes from two unrelated donors in culture. (wikipedia.org)
The whole blood is collected from non-transfused male donors of ABO type, AB. (labquip.net)

Similar results were obtained using biotinylated CMRF-56 mAb, an antibody identifying a comparable population in cultured PBMC. (nih.gov)
Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer Staining Set (cat. (thermofisher.com)
This antibody can be used at 5 µL (0.25 µg) per test. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)

A study was conducted to determine the mutagenic potential of test substance, Coco TMAC (33% active in water), according to OECD 471 and EPA OPPTS 870.5265 Guidelines, in compliance with GLP. (europa.eu)
Peripheral human lymphocytes are recommended in international guidelines (OECD, EC). (europa.eu)

However, the standard for graft matching now depends on a series of HLA-matching done with molecular typing methods. (wikipedia.org)
Both of these graft types also have a limited availability. (biomedcentral.com)

Similar studies conducted in laboratory animals exposed to high and low doses of PBBs (not specified) found a dose related lymphocyte dysfunction in the high dose group. (cdc.gov)
However, the diagnosis can be made upon the onset of ataxia and before the appearance of telangiectasia if confirmed by laboratory tests. (shengsci.com)

Pre-Experiment for Toxicity: To evaluate the toxicity of the test item a pre-experiment was performed with all strains used. (europa.eu)

We also measured IL-10 and IL-12 concentration in the supernatant of DC cultures. (torvergata.it)
In the pre-experiment the concentration rage of the test item was 3-5000 µg/plate. (europa.eu)

CGH also uses two chromosomes, a test sample, and a control sample. (psychesystems.com)
FISH locates the presence or absence of different types of chromosomes and strands of DNA by using fluorescent probes. (psychesystems.com)

Cultured peripheral human lymphocytes were used as test system. (europa.eu)
The other family members include herpes simplex virus type 1 (HSV-1 or HHV-1) and herpes simplex virus type 2 (HSV-2 or HHV-2), varicella zoster virus (VZV), human herpes virus (HHV)-6, HHV-7, and HHV-8. (medscape.com)

We investigated whether alterations in phenotype and function of DC may contribute to the pathogenesis of Type 1 diabetes (T1DM). (torvergata.it)
A followup study of 90 Michigan residents with high or low PBB exposures found all parameters of lymphocyte function to be normal. (cdc.gov)
The author concludes that PBBs are capable of altering lymphocyte function. (cdc.gov)
The clinical significance of the changes in lymphocyte function are unknown. (cdc.gov)
Asthma is a clinical diagnosis based on symptoms, detailed history and pulmonary function testing [ 1 ]. (ersjournals.com)
Hence, Hogg and colleagues, who also first used the term "small airway disease" [ 7 ], described the distal airways as the "silent zone" of the airway tree as extensive disease can be present without symptoms or changes in conventional lung function tests [ 14 ]. (ersjournals.com)

Lymphocytes were cultured for 48 ± 2 h and thereafter exposed in duplicate to selected doses of test substance for 3 h in the absence and presence of S9-mix. (europa.eu)

Based on the results of the three in vitro studies, the test substance is considered to have no genotoxicity potential. (europa.eu)

All samples were tested by amplification of a fragment of the 18S rDNA locus, and sequencing showed the presence of Cryptosporidium hominis ( n = 42), C. meleagridis ( n = 20), C. canis ( n = 12), C. felis ( n = 7), C. suis ( n = 6) and C. parvum ( n = 5). (biomedcentral.com)

Cell type being detected. (preferred-cell-systems.com)

After they are mixed, researchers can use a microscope to identify new hydrogen bonds created with the fluorescent probes. (psychesystems.com)

Each unit is tested and found negative for: HBsAg, HCV, HIV-1, HIV-2, HIV-1Ag or HIV 1-NAT, ALT, and syphilis by FDA-Approved Methods. (labquip.net)

Anatomy35

Organisms13

Diseases9

Chemicals and Drugs70

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes40

Disciplines and Occupations1

Information Science3

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Assay7

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Cells39

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Donor4

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Cytokines2

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Reactions4

Fibroblasts3

Assays1

Antigen6

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Immunosuppressive1

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