• RRM3 is located on chromosome 8 in yeast cells and codes for 723 amino acids producing a protein that weighs 81,581 Da. (wikipedia.org)
  • 2011) Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins. (yeastgenome.org)
  • Saccharomyces cerevisiae (Baker's yeast) rho-type GTPase activating protein RGA1/DBM1. (embl.de)
  • In order to construct yeast expressing vector for the tHSA-L7/L12 fusion protein, the L7/L12 ribosomal gene was amplified by PCR. (ac.ir)
  • We have used the pheromone alpha-factor receptor (Ste2p) of the yeast Saccharomyces cerevisiae as a model system to understand ligand binding, receptor activation, and G protein interaction. (tennessee.edu)
  • Coupling of cell identity to signal response in yeast: interaction between the alpha 1 and STE12 proteins. (embl-heidelberg.de)
  • A recently filed patent (Publication Number: US20230193338A1) describes a yeast cell that has been genetically modified to express specific proteins. (just-drinks.com)
  • The yeast cell contains two exogenous nucleic acids, one encoding a retrograde regulation protein (Rtg) and the other encoding a polypeptide. (just-drinks.com)
  • Overall, this patent presents a novel approach to genetically modifying yeast cells for the expression of specific proteins. (just-drinks.com)
  • The use of different promoter elements, transcriptional activators, and heme biosynthesis enzymes allows for precise control and optimization of protein expression in yeast cells. (just-drinks.com)
  • Yeast Krr1p physically and functionally interacts with a novel essential Kri1p, and both proteins are required for 40S ribosome biogenesis in the nucleolus. (nih.gov)
  • Herein, we used the amenable yeast Saccharomyces cerevisiae to study whether ubiquitin facilitates the expression of the fused eL40 (Ubi1 and Ubi2 precursors) and eS31 (Ubi3 precursor) ribosomal proteins. (123dok.net)
  • To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. (nature.com)
  • Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
  • c ) Overlap of mRNA interactome proteins in yeast and HuH-7. (nature.com)
  • d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
  • The yeast metal resistance proteins, which are 850-900 amino acyl residues in length, also exhibit two or three putative TMSs. (tcdb.org)
  • Overexpression of the yeast proteins, Al R 1p and Mn R 2p, overcomes toxicity to aluminum and manganese, respectively. (tcdb.org)
  • The yeast proteins appear to exhibit broad specificity transporting a wide range of di- and trivalent metal cations. (tcdb.org)
  • The yeast SM protein Vps45p regulates membrane fusion through the trans-Golgi / late endosomal system, and interacts directly with the syntaxin (Tlg2p) and the v-SNARE (Snc2p) proteins. (gla.ac.uk)
  • In the current study, we report a type of DSB-dependent mutation associated with Top1cc stabilization: large deletions (median size, ∼100 bp) with little or no homology at deletion junctions. (duke.edu)
  • Within the resolution of these immunodetection techniques, we show that proteins encoded by the SIR3, SIR4, and RAP1 genes colocalize in a statistically significant manner with Y' telomere-associated DNA sequences. (rupress.org)
  • Rrm3p is known to affect an estimated 1400 discrete replication fork sites in the S. cerevisiae genome, including sites at ribosomal DNA repeats, tRNA genes, centromeres, telomeres, G4 DNA and the silent mating-type loci. (wikipedia.org)
  • Rhombotin 1 (RBTN1 or TTG-1) and rhombotin-2 (RBTN2 or TTG-2) are proteins of about 160 amino acids whose genes are disrupted by chromosomal translocations in T-cell leukemia. (embl.de)
  • To further validate the results pathway study was performed to identify the involvement of the coding genes in type II diabetes. (researchgate.net)
  • In most eukaryotes studied, ubiquitin is encoded by two classes of genes: (i) The first comprises genes coding for a single copy of ubiquitin fused to ribosomal proteins (r-proteins), most commonly eL40 and eS31. (123dok.net)
  • Genes that encode the proteins of complement components or their isotypes are distributed throughout different chromosomes, with 19 genes comprising 3 significant complement gene clusters in the human genome. (medscape.com)
  • Saccharomyces cerevisiae S288C Cox15p (COX15), partial mRNA. (genscript.com)
  • The aim of this review was to discuss the current understanding of Type 2 Diabetes Mellitus (T2DM) genetic advance and aetiology. (scialert.net)
  • Silent Information Regulator Proteins, Saccharomyces cerevisiae" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
  • The secreted recombinant fusion protein was detected in supernatant by SDS-PAGE and confirmed by western blot analysis using anti-HSA and anti-L7/L12 antibodies. (ac.ir)
  • malonyl CoA-acyl carrier protein transacylase, putative [Geobacter sulf. (yeastrc.org)
  • Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synt. (yeastrc.org)
  • Vac8 controls vacuolar membrane dynamics during different autophagy pathways in Saccharomyces cerevisiae. (ruhr-uni-bochum.de)
  • The PI3K/Akt/mTOR signaling pathway is a key regulator of diverse physiological functions such as proliferation, global protein, and lipid synthesis as well as many metabolic pathways interacting to increase secretory capabilities. (go.jp)
  • S. cerevisiae hypothetical protein YKR090w. (embl.de)
  • Mammalian LH-2, a transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types. (embl.de)
  • A set of nuclear proteins in SACCHAROMYCES CEREVISIAE that are required for the transcriptional repression of the silent mating type loci. (jefferson.edu)
  • Impossible Foods has filed a patent for materials and methods that involve overexpression of a transcriptional activator called retrograde regulation protein 1 (Rtg1) to increase the expression of one or more polypeptides. (just-drinks.com)
  • RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
  • In this chapter, we will discuss the principle of heterologous gene expression, different types of hosts organism available for heterologous protein production. (benthamscience.com)
  • The smaller proteins are generally of prokaryotic origin while the larger ones are of eukaryotic origin. (tcdb.org)
  • The larger eukaryotic and archaeal proteins possess N- and C-terminal hydrophilic extensions. (tcdb.org)
  • Different promoter systems that are used for gene expression also influence protein production in case of both prokaryotic and eukaryotic hosts. (benthamscience.com)
  • Ubiquitin is a highly conserved small eukaryotic protein. (123dok.net)
  • Ubiquitin is a small eukaryotic protein of 76 amino acids whose name (i.e., it occurs ubiquitously) results from its remarkable evolutionary conservation [1]. (123dok.net)
  • SNARE proteins are essential components of the machinery that facilitates membrane fusion in eukaryotic cells. (gla.ac.uk)
  • Protein N-terminal (Nt) acetylation is one of the most abundant modifications in eukaryotes, covering ~50-80 % of the proteome, depending on species. (lu.se)
  • Mammalian and avian cysteine-rich protein (CRP), a 192 amino-acid protein of unknown function. (embl.de)
  • Mammalian cysteine-rich intestinal protein (CRIP), a small protein which seems to have a role in zinc absorption and may function as an intracellular zinc transport protein. (embl.de)
  • They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)
  • Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
  • Phosphorylation of the receptor protein Pex5p modulates import of proteins into peroxisomes. (ruhr-uni-bochum.de)
  • Literature data and our computational analysis revealed that Cdc34 protein present many consensus sites for CK2 phosphorylation. (unimib.it)
  • Through a thiolester assay, we studied Cdc34 (wild-type and Cdc34S130AS167A) binding to ubiquitin in vitro, and we observed that lack of CK2-mediated phosphorylation on S130 and S167 strongly reduced the ubiquitin-charging ability of Cdc34. (unimib.it)
  • In Saccharomyces cerevisiae a specialization of the two catalytic subunits (α e α') was shown: α subunit is involved in cell polarity, while α' subunit is linked to cell-cycle regulation and was shown to be fundamental both in G1 phase and in mitosis. (unimib.it)
  • In particular, among these, S130 and S167, within the catalytic domain of the protein, are highly conserved among Cdc34 homologues in various organisms, and were identified as phosphorylated sites in vivo in a CK2-dependent manner. (unimib.it)
  • This increase in recombination has been attributed to interactions between the protein Rrm3p and actual nucleotide base sequence from rDNA regions, rather than interactions due to the secondary structure formed by tandemly repeated DNA regions. (wikipedia.org)
  • RRM3 and its encoded protein have been shown to be vital for cellular replication, specifically associating with replication forks genome-wide. (wikipedia.org)
  • The proteins' G-quadruplex ability has been shown to reduce G4-related genome damage when there is low cellular levels of Pif1. (wikipedia.org)
  • The binding of these two proteins appears to be linked to inappropriate replication timing and genome integrity. (wikipedia.org)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
  • These results suggest that the iron bound to the Isa proteins is required for the de novo synthesis of [4Fe-4S] clusters in mitochondria and for their insertion into apoproteins in a reaction mediated by Iba57 . (yeastgenome.org)
  • We conclude that ubiquitin might serve as a cis-acting molecular chaperone that assists in the folding and synthesis of the fused eL40 and eS31 ribosomal proteins. (123dok.net)
  • In dry pollen, LARP6C binds to transcripts encoding proteins that function in lipid synthesis and homeostasis, vesicular trafficking, and polarized cell growth. (bvsalud.org)
  • One region of this protein, called the sterol reductase domain, plays an important role in the production (synthesis) of cholesterol. (medlineplus.gov)
  • During cholesterol synthesis, the sterol reductase function of the lamin B receptor allows the protein to perform one of several steps that convert a molecule called lanosterol to cholesterol. (medlineplus.gov)
  • Multi-pass membrane protein. (cusabio.com)
  • Ubiquitin functions as a reversible post-translational modifier of proteins to regulate many different cellular processes such as DNA repair, chromatin dynamics, cell cycle regulation, membrane and protein trafficking, endocytosis, autophagy, but most notably proteasome-dependent protein degradation [2-4]. (123dok.net)
  • The Sec1p / Munc18 (SM) family are a conserved group of proteins that regulate membrane fusion through interactions with their cognate syntaxins. (gla.ac.uk)
  • The important components of this system are various cell membrane-associated proteins such as complement receptor 1 (CR1), complement receptor 2 (CR2), and decay accelerating factor (DAF). (medscape.com)
  • Inner nuclear membrane protein LBR preferentially interacts with DNA secondary structures and nucleosomal linker. (medlineplus.gov)
  • Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
  • Inducible promoter elements, such as methanol-inducible promoter elements, can be used to regulate the expression of the proteins. (just-drinks.com)
  • These glycoproteins include the CD98 heavy chain protein of Mus musculus (gbU25708) and the orthologous 4F2 cell surface antigen heavy chain of Homo sapiens (spP08195). (tcdb.org)
  • The interaction of these two nuclear proteins is enhanced in the presence of salts, an indication that the interaction between the two is likely hydrophobic. (wikipedia.org)
  • The interaction between PCNA and Rrm3p suggests that Rrm3p may be an accessory DNA helicase that helps replication fork progression through formation of a protein-protein complex with PCNA. (wikipedia.org)
  • After ligand binding, signal transduction is mediated by the interaction of activated Ste2p with its G protein (Gpa1p). (tennessee.edu)
  • Formulation of unifying hypotheses describing how SM proteins function has been problematic, primarily due to the multiple modes of interaction that have been characterised for different members of this family binding to their cognate SNARE proteins. (gla.ac.uk)
  • In addition to investigating the functional significance of Vps45p's interaction(s) with Tlg2p, I have also investigated binding of the SM protein to the v-SNARE Snc2p. (gla.ac.uk)
  • The classical pathway is triggered by interaction of the Fc portion of an antibody (immunoglobulin [Ig] M, IgG1, IgG2, IgG3) or C-reactive protein with C1q. (medscape.com)
  • While proteomics typically aims to quantify as many proteins as possible, many of the biological questions that are to be addressed also necessitate the processing of large sample series. (elifesciences.org)
  • RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
  • Highly conserved acidic residues found in the short periplasmic loop are not essential for CorA function or Mg 2+ selectivity but may be required for proper protein folding and stability. (tcdb.org)
  • The CorA proteins of E. coli and S. typhimurium are each 316 amino acyl residues in length. (tcdb.org)
  • Taxonomic distribution of proteins containing STE domain. (embl-heidelberg.de)
  • b ) Volcano plot showing the distribution of proteins according to their enrichment in crosslinked (CL) over non-CL samples. (nature.com)
  • The gene codes for nuclear protein Rrm3p, which is 723 amino acids in length, and is part of a Pif1p DNA helicase sub-family that is conserved from yeasts to humans. (wikipedia.org)
  • We found that the FERM domain in the tail region, which is known to bind to lipids as well as other proteins, is essential for both nuclear and chloroplast positioning, whereas the proximal MyTH4 domain plays a supporting role in chloroplast transport. (go.jp)
  • For instance, how can cells control the diverse patterns of glycosylation of various secretory proteins in the endoplasmic reticulum and Golgi in an orderly manner with high accuracy? (go.jp)
  • Regulation of peroxisomal matrix protein import by ubiquitination. (ruhr-uni-bochum.de)
  • 2006), and Cdc34, the E2 enzyme required for the ubiquitination of many cell-cycle proteins (among which Sic1), which is phosphorylated by CK2 on Ser207, Ser216 e Ser282 (Pyerin et al. (unimib.it)
  • The K. lactis STE12 protein did not cooperate with the S. cerevisiae alpha 1 protein to promote the overall mating process or the induction of transcription of an alpha-specific gene. (embl-heidelberg.de)
  • Deficiency of any of these regulatory proteins results in a state of overactivation of the complement system, with damaging inflammatory effects. (medscape.com)
  • Ubiquitin fusion to other r-proteins, such as P1 or P2, and even to non-ribosomal proteins such as actin, has been reported in diverse genera of rare single-celled algae as, for example, Bigelowiella [6]. (123dok.net)
  • RRM3 is a gene that encodes a 5′-to-3′ DNA helicase known affect multiple cellular replication and repair processes and is most commonly studied in Saccharomyces cerevisiae. (wikipedia.org)
  • During cellular replication, cells encounter replication fork stalling due to DNA-protein complexes, DNA damage and secondary DNA structures. (wikipedia.org)
  • This type of replication stress is known as fork collapse. (wikipedia.org)
  • Rrm3p a DNA helicase that unwinds DNA in a 5'-to-3' polarity and has been shown to help DNA replication forks transverse protein-DNA complexes. (wikipedia.org)
  • There is some debate within the literature as to whether Rrm3p is part of the replisome or if it is a protein that is recruited to genomic sites upon pausing of replication forks. (wikipedia.org)
  • Here we employ a multi-dimensional proteomics approach to analyze Saccharomyces cerevisiae lacking NatA activity, which causes global proteome remodeling. (lu.se)
  • and Neisseria gonorrhoeae) to generate surface protein diversity, and by B lymphocytes of the vertebrate immune system to generate antibody diversity. (nih.gov)
  • Vertebrate insulin gene enhancer binding protein isl-1. (embl.de)
  • Vertebrate homeobox proteins lim-1, lim-2 (lim-5) and lim3. (embl.de)
  • Vertebrate paxillin, a cytoskeletal focal adhesion protein. (embl.de)
  • Thus, CNNM proteins, the vertebrate orthologues of CorB/C, also have Mg 2+ transport capacity. (tcdb.org)
  • The immunogenic B. abortus ribosomal protein L7/L12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. (ac.ir)
  • AF237571_1 malonyl CoA-acyl carrier protein transacylase precursor [Plasmo. (yeastrc.org)
  • It is generated by proteolytic cleavage of precursor proteins in which it is fused either to itself, constituting a polyubiquitin precursor of head-to-tail monomers, or as a single N-terminal moiety to ribosomal proteins. (123dok.net)
  • Isl-1 binds to one of the two cis-acting protein-binding domains of the insulin gene. (embl.de)
  • This suggests that telomeres are clustered in vegetatively growing cells, and that proteins essential for telomeric silencing are concentrated at their sites of action, i.e., at telomeres and/or subtelomeric regions. (rupress.org)
  • m6A mostly mediates its effect by recruiting reader proteins that either directly accommodate the modified residue in a hydrophobic pocket formed by their YTH domain, or otherwise have their affinity positively influenced by the presence of m6A. (bvsalud.org)
  • External application of synthetic peptides increases the abundance of the targeted protein, leading to related phenotypes. (bvsalud.org)
  • In vivo, large RNAs rely on proteins to fold to their native conformation. (nih.gov)
  • Most eukaryotes contain iron-sulfur cluster (ISC) assembly proteins related to Saccharomyces cerevisiae Isa1 and Isa2 . (yeastgenome.org)
  • The G protein-coupled receptor (GPCR) family is composed of hundreds of members and is expressed in eukaryotes. (tennessee.edu)
  • Understanding the role of the ubiquitin fused to ribosomal proteins becomes relevant, as these proteins are practically invariably eS31 and eL40 in the different eukaryotes. (123dok.net)
  • Among all of the noninsulin drugs currently in use to treat type 2 diabetes in adults, the US Food and Drug Administration (FDA) has approved metformin, liraglutide, and exenatide extended-release for use in children with type 2 DM. (medscape.com)
  • Some of these proteins exhibit 11 TMSs. (tcdb.org)
  • The greatest degree of conservation between homologues is found in TMSs 1 and 2 of the Thermotoga maritima protein. (tcdb.org)
  • Hydropathy analysis had predicted two transmembrane α-helical spanners (TMSs) in the C-terminal regions of these proteins. (tcdb.org)
  • The archaeal CorA protein is functionally similar to the CorA homologues of enteric bacteria. (tcdb.org)
  • Cytoskeleton is the pre-eminent supplier of Rho and Ras family small G-proteins (SGPs). (cytoskeleton.com)
  • Apart from the purified proteins, Cytoskeleton also provides some innovative kits for studying these proteins in cells and in vitro , see related products below for more information. (cytoskeleton.com)
  • Essentials of Recombinant Protein Production, Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications (2019) 1: 56. (benthamscience.com)
  • In addition to this, we will also elaborate on different aspects related to the overexpression and purification of recombinant heterologous protein and the type of tags and chromatographic techniques used for the purification of different types of proteins. (benthamscience.com)
  • Although Cdc14 activity is thought to be restricted to anaphase, we found that dephosphorylation of the Dsn1 kinetochore protein in metaphase requires Cdc14. (ox.ac.uk)
  • Pry3 short or full-length protein: where, when and how? (unifr.ch)
  • The crystal structure of the CorA homologue from Thermotoga maritima has been solved at 3.9 Å resolution for the full-length protein and at 1.85 Å resolution for the cytoplasmic domain ( Lunin et al . (tcdb.org)