Tumour tumor cells cultured. Medical search. Frequent questions

Breast cancer is one of the most common primary tumors to develop associated brain metastases ( 1 , 2 ), and while recent progress in cancer therapy has improved control of primary breast tumors, methods to effectively treat brain metastases from breast cancer remain inadequate. (jcancer.org)
A complete cell culture solution designed for the selective culture of malignant cells derived from primary tumors or patient-derived xenografts. (promocell.com)

During the metastasis, cancer cells in situ move to other tissues and organs from the blood circulation or the lymphatic system through proliferation, adhesion, invasion, and migration. (frontiersin.org)
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is a glycoprotein that has limited expression in normal adult tissues, but is overexpressed in carcinomas of the gastrointestinal tract, the genitourinary and respiratory systems, and breast cancer. (nih.gov)
The use of patient -derived tumor tissues and cells has led to significant advances in personalized cancer therapy and precision medicine . (bvsalud.org)
Western blotting was employed to determine NGF level in PC and paracarcinoma tissues and in PC cell lines as well as pancreatic ductal epithelial cells. (hindawi.com)
NGF level was preeminently higher in PC tissues and cell lines than in paracarcinoma tissues and normal pancreatic epithelial cell lines. (hindawi.com)
The tumor size and the number of proliferating cells were reduced in the tumor tissues obtained from the formononetin-treated group. (spandidos-publications.com)
Physiologically, the cell membranes of the body's tissues possess a variety of mechanisms through which substances on one side of a biomembrane may be selectively transported across to the other side. (weeksmd.com)
Tissues and Cells. (lu.se)

Here, we examined the anti-lung-metastatic effects and the mechanism of lycopene in combination with sorafenib in C57BL/6 mice xenografted with Lewis lung carcinoma (LLC) cells. (frontiersin.org)
The results showed that lycopene reduced the number of metastatic tumors in the lungs, which was further suppressed by the combined treatment with sorafenib. (frontiersin.org)
In anti-metastatic studies, lycopene has been found to inhibit the metastasis of human hepatoma SK-Hep-1 cells in athymic nude mice ( 17 ). (frontiersin.org)
SOX9 regulates cancer stem-like properties and metastatic potential exposed cells. (cdc.gov)
We also discovered that the invasiveness of metastatic colon cancer cells was alleviated following drug treatment. (spandidos-publications.com)
These findings suggest that formononetin inhibits angiogenesis and tumor cell invasion, and thus support its use in the treatment of advanced and metastatic colon cancers. (spandidos-publications.com)
spread of metastatic cells [3]. (researchgate.net)
In addition, we differentiate for the first time the contributions of ERBB1 and ERBB2 to the key metastatic properties of in vivo tumor cell invasion and intravasation. (aacrjournals.org)

2017. Rapamycin inhibits the secretory phenotype of senescent cells by a Nrf2-independent mechanism. . (oregonstate.edu)
2017. Divergent roles of p120-catenin isoforms linked to altered cell viability, proliferation, and invasiveness in carcinogen-induced rat skin tumors. . (oregonstate.edu)

Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)
MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and Transwell assays were employed to test cell proliferation, invasion, and migration, respectively. (hindawi.com)
Among its different constituents, Astragalus polysaccharides have been most widely studied particularly for their immunopotentiating properties, such as stimulation of murine B cell proliferation and cytokine production ( 6 ). (spandidos-publications.com)
Our results indicated that AST inhibits cell proliferation through phase-specific cell cycle arrest and caspase-dependent apoptosis. (spandidos-publications.com)
IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)
Experimental in vitro and in vivo research demonstrated decreased cell proliferation in desmoid cell cultures and small tumors when COX-2 was blockaded with pharmacologic agents. (medscape.com)

Thus, addressing the presence of immunosuppressive myeloid cell populations in the periphery and tumor microenvironment of patients with cancer is likely to be required for effective NK cell-based immunotherapy. (elifesciences.org)
However, a major limitation of this emerging technology is the absence of a tumor microenvironment that includes immune and stromal cells . (bvsalud.org)
By integrating cellular components of the tumor microenvironment , including T cells , into tumor organoid cultures , immuno-oncology has embraced this technology , which is rapidly advancing. (bvsalud.org)
Tumor growth is sustained by a highly diverse microenvironment, the TME. (promocell.com)
Through similar mechanisms, amino acid depletion in culture and cytokine activity in the tumor microenvironment prompt cancer cells to metastasize. (the-scientist.com)

Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. (frontiersin.org)
Immunostaining revealed that major melanoma-associated antigens, such as S100 protein, HMB-45, melanotransferrin, chondroitin sulphate proteoglycan, and the gangliosides GD2 and GD3, were consistently expressed by the tumour tissue, severe combined immunodeficiency (SCID) mouse xenograft and derived cell lines. (tmu.edu.tw)
This observation and the expression of the major melanoma-associated antigens were all indicative of the melanocytic origin of MST-1 tumour. (tmu.edu.tw)
The availability of the soft tissue melanoma cell line, the SCID mouse xenograft tumour system as well as autologous TILs described herein would provide useful materials for identifying T-cell-defined antigens as well as a model system for devising individualised cancer biotherapeutic strategies. (tmu.edu.tw)
Cell surface antigens are large molecules sticking out from a cell that help the immune system determine whether the cell is harmless or harmful. (stanford.edu)
This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)
Tumor Antigens Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)
Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)

Treatment of xenograft tumors resulted in PD-L1-dependent tumor growth inhibition. (elifesciences.org)

Two retrospective studies from Israel 12 and Japan 13 recently reported that diabetes was negatively correlated to overall survival in patients with non-small cell lung cancer (NSCLC) treated with ICIs. (nature.com)
We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote p.S668T mutation in nine of 42 samples and four of nine also had the p.D674H mutation. (scielo.br)
2004) Akt phosphorylation and gefitinib efficacy in patients with advanced non-small-cell lung cancer. (scielo.br)
On the basis of these preclinical data, the ADC SAR408701 is a promising candidate for development as a potential treatment for patients with CEACAM5-positive tumors. (nih.gov)
PD-L1 CAR haNKs reduced levels of macrophages and other myeloid cells endogenously expressing high PD-L1 in peripheral blood from patients with head and neck cancer. (elifesciences.org)
Exploring Tumor-Immune Interactions in Co-Culture Models of T Cells and Tumor Organoids Derived from Patients. (bvsalud.org)
With the development of tumor molecular heterogeneity theory(12), there are still some patients with GIST who are sensitive to radiotherapy, especially for the patients with advanced stage(13-16).In addition, with the development of imaging technology and modern tissue and organ radiation technology, it has become a reality to concentrate high-dose radiation locally in abdominal cavity tumors(12), which challenges RT's insensitivity to GISTs. (researchsquare.com)
Dr. Carl Schwartz, Chief Executive Officer of Precision Therapeutics, commented, "TumorGenesis is developing a better way to grow tumors outside the human body so they mimic the environment inside the patients' body as closely as possible. (globenewswire.com)
The TumorGenesis PDx model will initially be developed for three orphan cancers, Multiple Myeloma, Triple-Negative Breast cancer (TNBC) and Ovarian cancers, all of which are areas that have a high unmet need for new and effective treatments that are tailored to patients' unique tumor profiles. (globenewswire.com)
Fields et al reviewed 500 patients with Merkel cell carcinoma in a single-institution study. (medscape.com)
We recommend meloxicam for patients with extraabdominal desmoid tumors, instead of surgery first," said lead author Yoshihiro Nishida, MD, PhD, from the Department of Orthopedic Surgery at Nagoya University Graduate School of Medicine in Japan. (medscape.com)
In this study, between 1991 and 2003, Dr. Nishida and colleagues identified 30 patients who were surgically treated for extraabdominal desmoid tumors at their institutions (excluding those with intraabdominal, familial adenomatous polyposis-associated, and unresectable tumors). (medscape.com)
However, the significant morbidity of aggressive chemotherapy should be avoided in patients with extraabdominal desmoids tumors," they say. (medscape.com)
Glioblastoma multiforme (GBM) the highest-grade glioma and deadliest brain tumor occurs in pediatric as well as adult patients. (lu.se)

The epidermal growth factor receptor family (ERBB) is overexpressed in a wide variety of tumor types and is correlated with poor prognosis. (aacrjournals.org)

Cancer/testis antigen MAGEC2 (also known as HCA587) is highly expressed in a wide variety of tumors and plays an active role in promoting growth and metastasis of tumor cells. (biomedcentral.com)

Tumor cells with stem cell characteristics are thought to be responsible for therapeutic resistance in brain tumors. (lu.se)
Using mouse models of glioma and primary human glioma cultures, we aim to characterize phenotypic intratumoral heterogeneity specifically with regards to radiation resistant stem-like tumor cells, the molecular signaling pathways underlying therapeutic resistance, and microenvironmental control over tumor cell phenotypes with the overall goal of developing novel therapeutic strategies targeting therapy-resistant cells in malignant brain tumors. (lu.se)

Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)
SUMMARY BLURB Essential oil derived from the camphor tree acts by stimulating immune cell-dependent regression of skin tumors in a mouse model of cutaneous squamous cell carcinoma. (biorxiv.org)

Overall, the present study demonstrates that lycopene in combination with sorafenib additively inhibits the lung metastasis of tumor, indicating lycopene has potential as an adjuvant for sorafenib in cancer treatment. (frontiersin.org)

January 5, 2010 - Meloxicam ( Mobic ), a cyclooxygenase (COX)-2 inhibitor drug used for arthritis, has shown promise as a treatment for extraabdominal desmoid tumors, according to the results of a small pilot study. (medscape.com)
Extraabdominal desmoid tumors are generally sporadic in nature and can occur in virtually any body site, note the authors. (medscape.com)
Baseline imaging of desmoid tumors by magnetic resonance imaging (MRI) was obtained prior to beginning treatment with oral meloxicam 10 mg/day. (medscape.com)
Surgery remains the mainstay for resectable sporadic desmoid tumors, especially extraabdominal ones, the authors write, and there is growing evidence that chemotherapy might be effective in managing life-threatening intraabdominal desmoid tumors. (medscape.com)

The therapeutic efficacy of immune checkpoint inhibitors has underscored the importance of immune cells , particularly cytotoxic T cells that infiltrate the vicinity of tumors , in patient prognosis . (bvsalud.org)
These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
T-cell engagers are bispecific antibodies that recruit cytotoxic T cells to kill tumor cells. (msdmanuals.com)

Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. (elifesciences.org)

This study supports ERV-K env as a single prognostic indicator for better survival of RCC, which we propose represents a new tumor antigen. (frontiersin.org)
Failed T cell-based immunotherapies in the presence of genomic alterations in antigen presentations pathways may be overcome by NK cell-based immunotherapy. (elifesciences.org)
T cell-based immunotherapy, such as immune checkpoint blockade or adoptive T cell transfer, is limited by the ability of T cells to detect major histocompatibility complex (MHC)-presented antigen by tumor cells. (elifesciences.org)
Natural killer (NK) cell-based immunotherapy may overcome genetic mechanisms of resistance to T cell-based immunotherapy through antigen- and MHC-independent recognition of malignant cells. (elifesciences.org)
Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)
In mice whose brainstems were implanted with human DIPG, engineered immune cells known as chimeric antigen receptor T cells - or CAR-T cells - were able to eliminate tumors, leaving very few residual cancer cells. (stanford.edu)
In CAR-T therapies now used in humans, some of the patient's own immune cells are removed, engineered to attack a surface antigen on the cancer cells, and returned to the patient's body, where they target the cancer cells for destruction. (stanford.edu)
Clear cell acanthoma usually stains positively for epithelial membrane antigen and negatively for carcinoembryonic antigen. (medscape.com)
Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)

As such, CEACAM5 is an attractive target for antibody-based therapies designed to selectively deliver cytotoxic drugs to certain epithelial tumors. (nih.gov)
For VEGF, the target of current therapies, this correlation between hypoxia and higher cytokine levels was greater in primary breast and lung carcinoma cells than in ovarian carcinoma cells or tumor cell lines. (biomedcentral.com)
Even though the idea of an immune system actively searching for and disposing of potential mutated tumor cells is over a century old, only recent developments in various fields such as mass spectrometry, immuno-checkpoint blockade strategies and in silico modelling have enabled the realization of the full potential of recruiting immune system to fight cancer and the possibilities of personalized therapies. (helsinki.fi)
Clinical trials of LAK cells in humans are ongoing but this approach has not gained widespread use and is generally considered less effective than other cell therapies. (msdmanuals.com)

TAK-285 was examined in a model of breast cancer brain metastasis using direct intracranial injection of BT-474-derived luciferase-expressing cells and showed greater inhibition of brain tumor growth compared to animals treated with lapatinib. (jcancer.org)
Treatment of syngeneic tumors resulted in CD8 and PD-L1-dependent tumor rejection or growth inhibition and a reduction in myeloid cells endogenously expressing high levels of PD-L1. (elifesciences.org)
Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
Our studies were designed to evaluate the effect of ERBB inhibition on tumor cell motility and intravasation in vivo using clinically relevant small-molecule inhibitors. (aacrjournals.org)
ERBB1 and ERBB2 inhibition rapidly (within 3 h) inhibits both tumor cell motility and intravasation. (aacrjournals.org)
Using gefitinib, ERBB1 inhibition rapidly inhibits tumor cell motility and invasion but not intravasation, whereas ERBB2 inhibition by AG825 rapidly blocks intravasation. (aacrjournals.org)
ERBB1 and ERBB2 inhibition can rapidly block tumor cell invasive properties. (aacrjournals.org)
These results indicate that ERBB inhibition should be considered for blocking other tumor cell malignant properties besides growth. (aacrjournals.org)

SOX9 depletion also suppressed the formation of cancer stem-like cells (CSCs), as determined by tumor sphere formation and aldehyde dehydrogenase (ALDH) activity (Aldefluor) assays. (cdc.gov)
In addition to its proprietary precision diagnostics for oncology, Helomics offers boutique CRO services that leverage our patient-derived tumor models, coupled to a wide range of multi-omics assays (genomics, proteomics and biochemical), and a proprietary bioinformatics platform (D-CHIP) to provide a tailored solution to our client's specific needs. (globenewswire.com)

Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
Angiogenesis is essential in the process of carcinogenesis to facilitate tumor progression and metastasis. (spandidos-publications.com)
Another important mechanism for the progression of colorectal cancer is the ability of the transformed cells to invade and traverse the basement membrane, which in turn facilitates the conversion of adenomas into carcinomas and then metastases ( 1 ). (spandidos-publications.com)
Approaches to characterize tumor development, progression, and metastasis in these models using state-of-the-art imaging, histopathological, surgical, and other techniques are also included. (cshlpress.com)
While studying the progression of healthy cells into cancerous ones, researchers discover a way to engraft human blood cells into animals. (the-scientist.com)

Immune checkpoint inhibitors (ICIs), including antibodies against programmed cell death-1 (PD-1) and PD-1 ligand-1 (PD-L1), induce their anti-tumor effects by improving the function of immune cells 11 . (nature.com)
To investigate the effect of hyperglycemia on anti-tumor function induced by anti-PD-1 antibody treatment, we treated STZ-induced diabetic mice with subcutaneous MC38 colon carcinoma tumors with anti-PD-1 antibody, and examined changes in tumor growth, the number of tumor-infiltrating immune cells, and the expression of chemokines and chemokine receptors. (nature.com)
Although epigenetically silenced, endogenous retroviral (ERV) genes become activated in tumors and function to ignite immune responses. (frontiersin.org)
Metz H, Busch SE, Hanke ML, Kargl J, Kim KH and Houghton M (2014) Insulin receptor substrate-1 regulates immune cell content in lung adenocarcinoma. (scielo.br)
In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)
Engineered human immune cells can vanquish a deadly pediatric brain tumor in a mouse model, a study from the Stanford University School of Medicine has demonstrated. (stanford.edu)
However, some mice experienced dangerous levels of brain swelling, a side effect of the immune response triggered by the engineered cells, the researchers said, adding that extreme caution will be needed to introduce the approach in human clinical trials. (stanford.edu)
Crystal Mackall says she is encouraged that the work in mice showed that engineered immune cells called CAR-T cells were able to eradicate the tumors in mice. (stanford.edu)
In addition, the ability of radiotherapy to stimulate both innate and adaptive immune cells' function has been shown to enhance the therapeutic benefit of immunotherapy in some cases. (unil.ch)
Now we need to quantify more protein biomarkers, assess the tumor mutational landscape, and examine immune cell signatures, too. (the-scientist.com)
Additional information of PD-L1 expression can be used to evaluate cell line's immune-evasion abilities. (helsinki.fi)

Mechanistically, both peptides caused a significant reduction at G0/G1 phase, in correlation with an augmented expression of the cell cycle inhibitory proteins p53, p27, p21, necrosis, exacerbated generation of reactive oxygen species and diminished mitochondrial membrane potential, all hallmarks of cellular stress. (nature.com)
A localized hypoxic environment occurs during tumor growth necessitating an angiogenic response or tumor necrosis results. (biomedcentral.com)
Tumour necrosis factor (TNF)-α is thought to be a key early cytokine in the pathogenesis of sarcoidosis, despite conflicting data. (ersjournals.com)
This study has demonstrated that a subset of mononuclear phagocytes, mature macrophages, are responsible for tumour necrosis factor secretion and this could have implications for targeted management in sarcoidosis in the future. (ersjournals.com)
Tumour necrosis factor (TNF)-α is a key early cytokine in a variety of chronic inflammatory responses, including fibrosing alveolitis and granulomatous disease 6 - 8 . (ersjournals.com)

The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)

The Tasmanian devil faces extinction due to devil facial tumour disease (DFTD), a highly transmittable clonal form of cancer without available treatment. (nature.com)
The Tasmanian devil is an endangered Australian species, restricted to the island state of Tasmania 1 , and threatened with extinction due to a contagious and transmissible "parasitic" form of cancer known as devil facial tumour disease (DFTD) 2 , which has killed ~80% of the devil population since emerging in 1996 1 . (nature.com)
To this topic, lycopene had been demonstrated to have synergism from the combination of oxaliplatin in a human ovarian cancer cell line ( 20 ), suggesting that the adjuvant property on the anticancer of lycopene. (frontiersin.org)
Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
Consistently, the essential oil represses signaling pathways and cell cycle regulators that have been proposed as therapeutic targets for breast cancer. (biomedcentral.com)
Lung cancer is divided into two major groups: non small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (scielo.br)
2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)
We tested an essential-oil derivative, camphor white oil (CWO), for anti-tumor activity in a mouse model of keratinocyte-derived skin cancer. (biorxiv.org)
The three-dimensional tumor organoids derived from self -organizing cancer stem cells are valuable ex vivo models that faithfully replicate the structure, unique features, and genetic characteristics of tumors . (bvsalud.org)
This review focuses on the current applications of organoid - T cell co-culture models in cancer research and highlights the remaining challenges that need to be addressed for its broader implementation in anti- cancer therapy . (bvsalud.org)
This cell line can also be used for further studies aimed at uncovering the histogenesis of this rare cancer. (tmu.edu.tw)
Novel cancer treatment strategies take advantage of tumor-induced vascularisation by combining standard chemotherapeutic agents with angiogenesis-inhibiting agents. (biomedcentral.com)
Our previous findings demonstrated that formononetin initiates growth-inhibitory and pro-apoptotic activities in human colon cancer cells. (spandidos-publications.com)
In the present study, we aimed to further examine the potential of formononetin in controlling angiogenesis and tumor cell invasiveness in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
Additionally, the expression and activity of matrix metalloproteinases (MMPs) are increased in almost every type of human cancer, which may be associated with cell invasion, metastasis and angiogenesis. (spandidos-publications.com)
In recent years, we focused on investigating the anti-carcinogenic properties of total Astragalus saponins (AST) in human colon cancer cells and tumor xenografts. (spandidos-publications.com)
Subsequent study using multiple human cancer cell lines, including those of the colon, stomach and liver, further indicated the universal growth-inhibitory and pro-apoptotic effects of AST. (spandidos-publications.com)
Using in vivo mouse models of breast cancer, we test the effects of ERBB1 and ERBB2 inhibitors AC480 and lapatinib, ERBB1 inhibitor gefitinib, and ERBB2 inhibitor AG825 on in vivo tumor cell invasive properties in mammary fat pad tumors. (aacrjournals.org)
However, little is known for the regulation of MAGEC2 expression in cancer cells. (biomedcentral.com)
Our findings show that TRIM28 is necessary for MAGEC2 expression in cancer cells, and TRIM28 may serve as a new potential target for immunotherapy of cancer. (biomedcentral.com)
In mice, a fatal brainstem tumor was cleared by injecting it with engineered T cells that recognized the cancer and targeted it for destruction. (stanford.edu)
When the brains of the mice were examined via immunostaining after treatment, the animals had, on average, a few dozen cancer cells left, compared with tens of thousands of cancer cells in animals that received a control treatment. (stanford.edu)
As a cancer immunotherapist, what gets me really excited is when you take an established tumor and you make it disappear," said Crystal Mackall , MD, professor of pediatrics and of medicine and the study's other senior author. (stanford.edu)
Scientists have known for decades that GD2 levels on some other forms of cancer are very high, but its discovery on this tumor came as a surprise, Mackall said, adding, "It was hiding in plain sight, and we didn't know. (stanford.edu)
Other CAR-T cells that were tuned to different molecular targets also did not kill the DIPG cancer cells. (stanford.edu)
New monoclonal antibodies kill both cancer-promoting immunosuppressive cells and tumor cells in culture. (the-scientist.com)
Following a spate of patient deaths in clinical trials testing modified T cells for the treatment of cancer, researchers work to reduce the treatment's toxicity without sacrificing efficacy. (the-scientist.com)
This follows a license agreement with SyntArray, LLC, announced on March 13th , and is the latest milestone in the Company's strategy to bring together ground-breaking technologies to develop the next generation of patient derived ("PDx") tumor models for precision cancer therapy and drug development. (globenewswire.com)
Once identified by 48HD's technology, SyntArray's targeted peptide cell capture technology screens these peptides to determine the best combination that will capture the cancer cells and allow them to attach and grow on the 3D biomimetic support. (globenewswire.com)
Preliminary MTS assay is used to determine linear range and optimal time frame for the PBMC/cancer cell co-culture killing assay. (helsinki.fi)
Another challenge is the cell entry of oncolytic adenovirus, which is mainly mediated by the Coxsackie-Adenovirus receptor and this receptor is downregulated in various cancer cells. (helsinki.fi)
In order to overcome these limitations, this study explored the possibility to use cancer cell derived extracellular vesicles (EVs) as drug delivery system for oncolytic adenovirus. (helsinki.fi)
Animal studies have shown that LAK cells are more effective against cancer cells than are the original endogenous T cells, presumably because of their greater number. (msdmanuals.com)
Therefore CAR T cells and TCR T cells may represent complementary approaches to cancer therapy. (msdmanuals.com)
CAR T cell immunotherapy for human cancer. (msdmanuals.com)
arations verified the presence of cancer cells in all samples. (lu.se)

Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)

Western blot analysis was performed to study Boswellia sacra essential oil-regulated proteins involved in apoptosis, signaling pathways, and cell cycle regulation. (biomedcentral.com)
Generally, it is believed that there are four main pathways between tumor invasion and metastasis, including local invasion, blood, lymphatic, and seeding metastasis [ 6 ]. (hindawi.com)
In addition to these four pathways, tumors can undergo infiltration around nerve fibers and metastasis along nerves, that is, perineural invasion (PNI), which refers to the phenomenon of perineural invasion by tumor cells filling the perineurial space, wrapping around nerves in a continuous concentric sheath-like pattern, infiltration, and metastasis of extension along nerves around nerve fibers or into perineurium within the perineurium [ 7 ]. (hindawi.com)

The innovative 48HD technology allows us to capture all the heterogeneity of the tumor, including both the cancerous and non-cancerous cells. (globenewswire.com)

Pathologically, at an early stage, there is an influx of mononuclear cells followed by lymphocytes. (ersjournals.com)
Tumor-infiltrating lymphocytes (TILs) may have greater tumoricidal activity than LAK cells. (msdmanuals.com)

Tumors' mutations can encode the seeds of their own destruction, in the form of immunogenic peptides recognized by T cells. (the-scientist.com)

Specifically, ERV-K members were originally identified due to their similarity to the Mouse Mammary Tumor Virus (MMTV) and are subdivided into ten so-called human MMTV-like clades (HML1-10) ( 2 , 3 ). (frontiersin.org)
Ma Z, Gibson SL, Byrne MA, Zhang J, White MF and Shaw LM (2006) Suppression of insulin receptor substrate 1 (IRS-1) promotes mammary tumor metastasis. (scielo.br)
Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)

In vitro studies have shown that stromal cells cultured in hypoxic growth conditions secrete higher levels of critical angiogenesis-inducing factors than cells cultured in normoxic conditions [ 3 ]. (biomedcentral.com)

The exact biological mechanism(s) by which the BBB limits drug exposure is not clear, but in addition to epithelial tight junctions, enhanced efflux of chemotherapeutic agents out of the CNS space by endothelial cell membrane transporters in brain blood vessels plays a primary role ( 2 ). (jcancer.org)
The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)

We analyzed the effect of PD-1-Ab on tumor growth in streptozotocin-induced diabetic mice (STZ-mice) subcutaneously inoculated with MC38 (a colon carcinoma cell line). (nature.com)
Furthermore, we assessed the expression of chemokines by polymerase chain reaction (PCR) array in tumor-draining lymph nodes (dLNs) of these mice and MC38 cells cultured in different glucose concentrations. (nature.com)
This was accompanied by fewer tumor-infiltrating CD8 + T cells, and STZ-mice had fewer tumor-infiltrating CD11c + dendritic cells (DCs) than normoglycemic mice. (nature.com)
mRNA expression levels of CXCL9, a chemokine recruiting CD8 + T cells, were lower in dLNs of STZ-mice than in normoglycemic mice after PD-1-Ab treatment, and its protein was expressed in DCs. (nature.com)
To induce hyperglycemia, mice were intraperitoneally injected with STZ, which can damage pancreatic β cells resulting in the development of insulin dependent diabetes, a model of type 1 diabetes mellitus (T1DM). (nature.com)
Mice were intraperitoneally injected with STZ or sodium citrate buffer (CB) 7 days before subcutaneous inoculation with MC38 cells in the right flank on day 0 (Fig. 1 A). Then, the mice were treated with anti-PD-1 antibody or normal rat IgG on days 5, 8, and 11. (nature.com)
The effect of anti-PD-1 antibody on MC38 tumor is attenuated in STZ-induced diabetic mice. (nature.com)
Subsequently, we used a model of neuroinvasion in nude mice to assess the effect of NGF in vivo on tumor nerve invasion as well as on nociceptive transmission. (hindawi.com)
The reduction in tumor volume as well as pro-apoptotic and anti-proliferative effects in HT-29 nude mice xenograft are comparable to those produced by 5-FU. (spandidos-publications.com)
The study , which was published online April 16 in Nature Medicine, represents the first time a severe human brainstem tumor, diffuse intrinsic pontine glioma, has been eradicated in mice. (stanford.edu)
Next, the team tested the GD2 CAR-T cells in mice whose brainstem was implanted with human DIPG tumors, an experimental system that Monje's lab pioneered. (stanford.edu)
Stimulation of d endritic cell activation was also able to promote a potent antitumor effect with 70% complete response and increased overall survival of mice implanted with an orthotopic head & neck tumor. (unil.ch)
Mice engineered to overproduce the organelles involved in cell division spontaneously develop malignancies. (the-scientist.com)

The LAK cells produced are then returned to the patient's bloodstream. (msdmanuals.com)

Interleukin-2 (IL-2) expanded TILs had the predominant CD8 + phenotype and the capacity to lyse cells of the cultured autologous tumour. (tmu.edu.tw)
Next, using a lung adenocarcinoma model (KP), we demonstrated that modulation of neutrophil phenotype was able to enhance the tumor response to radiation therapy without any stimulation of fibrosis. (unil.ch)

Moreover, we recently reported that lycopene inhibits the metastasis of human liver adenocarcinoma SK-Hep-1 cells by downregulation of the NADPH oxidase 4 (NOX4) proteins ( 18 ). (frontiersin.org)
We postulated that the expression levels of angiogenesis-related proteins from various primary tumor cultures would be greater under hypoxic conditions than under normoxia. (biomedcentral.com)
In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)

Flow cytometric analysis of the tumour DNA content showed an index of 1.8 relative to normal peripheral blood lymphocyte DNA. (tmu.edu.tw)
Flow cytometric analysis provided essential information of the cell line HLA-1 expression. (helsinki.fi)

This has led to investigations of both cytotoxic and noncytotoxic chemotherapy, but because the tumors are rarely fatal, finding a pharmacologic treatment with fewer complications is desirable, according to the authors. (medscape.com)
Despite aggressive treatment with surgery, irradiation and sometimes chemotherapy, tumors invariably recur as incurable lesions. (lu.se)

Case reports in the literature report clear cell acanthomas with multiple lesions, (ie, polypoid clear cell acanthoma). (medscape.com)

The metastasizing cells penetrate the blood vessel wall by invasiveness, and migrate in the circulation, extravasate out of the blood vessels, colonize in the distant tissue, initiate angiogenesis, and finally grow at the new site ( 2 , 3 ). (frontiersin.org)
In this investigation a human RCC tissue microarray (TMA) (n=374) predominantly consisting of the most common histological tumor subtypes was hybridized with an ERV-K env antibody and correlated with patient clinical data. (frontiersin.org)
The establishment and characterisation of paired autologous tumour cell line (MST-1) and tumour-infiltrating lymphocyte (TIL) culture from a tumour mass of a 14-year-old Taiwanese girl with soft tissue melanoma are described. (tmu.edu.tw)
As a tumor grows, the existing blood supply becomes inefficient at supporting the tissue, and areas of the tumor become hypoxic. (biomedcentral.com)
Our collective goal is to find innovative tools - pharmacological or technological - able to protect normal tissue and enhance tumor control. (unil.ch)
Newer approaches are looking instead for ways of altering the pharmacokinetics of established drugs, particularly with respect to improving the ability of these to penetrate cell membranes at the end-organ/tissue level. (weeksmd.com)
However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)

MST-1 cells grown in vitro were heterogeneous in morphology, ranging from floating round cells, loosely attached round/oval or elongated cells with prominent pseudopod-like processes, to well-attached spindle and elongated dendritic cells without obvious pseudopods. (tmu.edu.tw)

Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. (frontiersin.org)

These tumor organoids have emerged as innovative tools that are extensively employed in drug testing, genome editing , and transplantation to guide personalized therapy in clinical settings. (bvsalud.org)
Clinical studies using ERBB inhibitors have focused on tumor growth effects, but ERBBs can contribute to malignancy independent of their effects on tumor growth. (aacrjournals.org)
similar anti-GD2 CAR-T cells are now being tested in clinical trials in a few other cancers. (stanford.edu)
The treatment and diagnosis of many cutaneous tumors is continually changing, and the role of clinicians (eg, primary care physicians, dermatologists, plastic surgeons, pathologists) focuses on an accurate diagnosis and a thorough understanding of the clinical sequelae. (medscape.com)
Typically, the clinical presentation of clear cell acanthoma is a solitary nodule in the lower extremities. (medscape.com)
To obtain an independent test set, the remaining 11 of the 58 tumors were selected from an ongoing clinical trial and used here as a blinded sion via ER,3 however the details of the estrogen effect on down- test set. (lu.se)

The reverse haemolytic plaque assay allows the measurement of cytokine production at a single cell level. (ersjournals.com)
The current authors have previously demonstrated, using a reverse haemolytic plaque assay (RHPA), that single-cell production of cytokine can be assayed 16 . (ersjournals.com)
This technique has an advantage over routine culture conditions, as the development of cytokine product by individual cells can be localised and measured, which therefore allows for the correlation of cytokine production with cell type. (ersjournals.com)

To reveal that the NGF-TrkA signaling pathway was closely associated with PC PNI, in vitro neuroinvasion model was established by using MiaPaCa-2 cells via coculturing DRG cells in Matrigel. (hindawi.com)
As linear correlations exist between expression levels of angiogenic factors under normoxic and hypoxic conditions in vitro , we propose that explanted primary cells may be used to probe the in vivo hypoxic environment. (biomedcentral.com)
Indeed, SOX9 knockdown inhibited anchorage-independent cell growth in vitro and lung colonization in vivo in a mouse xenograft model. (cdc.gov)

A greater number of alveolar macrophages produced TNF-α compared to autologous monocytes in sarcoidosis but not in controls and, based on cell size, it was confirmed that this was the product of more mature macrophages and that the secretion of TNF-α by monocytes and macrophages was heterogeneous: not all monocytes and macrophages secrete TNF-α. (ersjournals.com)
The hormone is made in the beta cells of the pancreas, and the stimulus for its secretion into the blood stream is a rise in the blood glucose concentration. (weeksmd.com)

The advent of genomic sequencing technologies has enabled the comprehensive analysis of tumor characteristics. (bvsalud.org)

Visit the Brain Tumor Biology groups research portal via this link. (lu.se)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Microarrays were prepared in tumor biology. (lu.se)

Furthermore, SOX9 knockdown suppressed tumor metastasis and the expression of the stem cell marker ALDH1A1. (cdc.gov)

Characteristics of cultured desmoid cells with different CTNNB1 mutation status. (dtrf.org)
In this study, we isolated three kinds ofÂ desmoidÂ cell with different CTNNB1 status, and compared their characteristics. (dtrf.org)
TumorGenesis is developing a new approach to growing tumor models in the laboratory that is faster, less costly and more closely mimics the characteristics of the patient's tumor, than the traditional PDx animal models that are currently on the market. (globenewswire.com)

In mouse and rat xenograft tumor models, TAK-285 showed antitumor activity against cancers that expressed HER2 or EGFR. (jcancer.org)
Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. (frontiersin.org)
2011. NADPH oxidase overexpression in human colon cancers and rat colon tumors induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). . (oregonstate.edu)
This article focuses mainly on nonmelanoma skin cancers, with an emphasis on rare benign and malignant epithelial, dermal, and adnexal tumors. (medscape.com)

Despite the crucial role of radiation resistance in patient mortality, few viable therapeutic strategies have been identified to target these resistant cells. (lu.se)

Mechanistically, NGF could upregulate TDE-miR-21-5p levels, and DRG cells took up TDE to activate the Warburg effect and stimulate nociceptor gene expression. (hindawi.com)
The vascular endothelial growth factor (VEGF) is a well characterized angiogenic factor known to stimulate angiogenesis within a tumor ( 1 ). (spandidos-publications.com)

however, mechanisms through which these bioactive compounds act on epithelial cells have not been identified. (biorxiv.org)
In this study, we linked SOX9, a stem cell associated transcription factor, to the neoplastic-like properties of human lung epithelial cells chronically exposed to a low-dose of single-walled carbon nanotube s (SWCNTs). (cdc.gov)

Cutaneous tumors encompass a vast array of malignancies, from neuroendocrine to lymphoid. (medscape.com)
In addition, these rare cutaneous tumors are often difficult to diagnose based solely on the physical examination findings, and the proper diagnosis relies on excisional biopsy with histologic studies. (medscape.com)
The origin of cutaneous tumors can be simplistically divided into 3 major categories: epidermal, dermal, and adnexal (see image below). (medscape.com)
On the other hand, clear cell acanthoma is a rare, benign epithelial cutaneous tumor. (medscape.com)

In MC38 cells cultured with 25 mM glucose, mRNA expression of CCL7, a chemokine recruiting DCs, was decreased compared to cells cultured with 5 mM glucose. (nature.com)
The expression level of MAGEC2 protein was significantly reduced when TRIM28 was depleted in tumor cells, and no changes were observed in MAGEC2 mRNA level. (biomedcentral.com)

however, it remains unknown whether the anti-tumor effects of anti-programmed cell death-1 antibody (PD-1-Ab) treatment are changed in hyperglycemic conditions. (nature.com)

NGF promoted MiaPaCa-2 and CFPAC-1 cell invasion and migration, while Tanezumab treatment showed the opposite results. (hindawi.com)
TrkA blocking-up could restrain NGF induced PC cell migration and neural invasion. (hindawi.com)
In vivo tumorigenesis experiments, Tanezumab markedly alleviated nerve invasion of PC cells as well as relieved nociceptive conduction in animal models. (hindawi.com)
These experiments temporally and molecularly separate two key stages in tumor cell entry into blood vessels: invasion and intravasation. (aacrjournals.org)

In addition, we designed and screened a panel of gomesin analogues with amino acid modifications that were predicted to influence cell viability. (nature.com)

In silico methods were successfully used to characterize previously identified HLA-restricted peptides and one previously identified immunogenic T-cell epitope. (helsinki.fi)

The cytotoxic activity of SAR408701 was assessed in CEACAM5-expressing tumor cell lines and using patient-derived xenograft mouse models of CEACAM5-positive tumors. (nih.gov)
Of interest, patient cell isolates differed in the precise pattern of elevated cytokines. (biomedcentral.com)
In passive cellular immunotherapy, specific effector cells are directly infused and are not induced within the patient. (msdmanuals.com)
Patient sex, tumor size, tumor site, follow-up interval, and period of medication were not significant prognostic factors of responsiveness. (medscape.com)

High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. (frontiersin.org)

Anatomy22

Organisms11

Diseases86

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Phenomena and Processes48

Disciplines and Occupations1

Information Science4

Health Care8

Primary tumors2

Tissues8

Metastatic8

20172

Proliferation8

Microenvironment5

Antigens8

Xenograft Tumors1

Patients14

Types and is correlated1

Growth and metastasis1

Brain tumors2

Skin tumors2

Metastasis of tumor1

Desmoid Tumors4

Cytotoxic T cel3

Murine1

Antigen9

Therapies4

Inhibition8

Assays2

Progression5

Immune11

Necrosis5

Phenotypes1

Cancer39

Genes1

Pathways3

Heterogeneity1

Lymphocytes2

Mutations1

Mammary3

Stromal cells1

Endothelial cell2

Mice13

Returned to the patient's1

Phenotype2

Proteins3

Cytometric analysis2

Chemotherapy2

Lesions1

Tissue7

Dendritic cells1

Histological1

Clinical6

Cytokine3

Vitro3

Secretion2

Genomic1

Biology3

Knockdown1

Characteristics3

Cancers4

Radiation1

Stimulate2

Epithelial cells2

Cutaneous4

MRNA2

Antibody1

Invasion4

Viability1

Characterize1

Patient4

Subtypes1