• brain tumors
  • Conversely, the presence of AGT protein in brain tumors predicts poor response to temozolomide and these patients receive little benefit from chemotherapy with temozolomide. (wikipedia.org)
  • Neutron capture therapy (NCT) is a noninvasive therapeutic modality for treating locally invasive malignant tumors such as primary brain tumors and recurrent head and neck cancer. (wikipedia.org)
  • BNCT has been evaluated clinically as an alternative to conventional radiation therapy for the treatment of malignant brain tumors (gliomas), and recurrent, locally advanced head and neck cancer. (wikipedia.org)
  • W. H. Sweet, from Massachusetts General Hospital, first suggested the technique for treating malignant brain tumors and a trial of BNCT against the most malignant of all brain tumors, glioblastoma multiforme, using borax as the delivery agent in 1951. (wikipedia.org)
  • epigenetic
  • Importantly, glucose deprivation and hypoxia were also found to enhance the resistance level of ABCG2-overexpressing resistant cells with pre-existing genetic and epigenetic MDR mechanisms. (hindawi.com)
  • In some tumors, epigenetic silencing of the MGMT gene prevents the synthesis of this enzyme, and as a consequence such tumors are more sensitive to killing by temozolomide. (wikipedia.org)
  • vitro
  • Cells grown in vitro from neoplastic tissue. (jove.com)
  • These advances may be useful in the future for the development of an in vitro cell culture model, allowing lung cancer cells at di erent stages of transformation to be studied. (docplayer.net)
  • Deficiency in growth factors or presence of cell growth inhibitors in serumsupplemented medium could explain the poor in vitro growth of these lung cancer and normal epithelial cells. (docplayer.net)
  • Overexpression of ABCG2 in cancer cell lines in vitro has been shown to confer MDR to a variety of anticancer drugs including mitoxantrone, irinotecan, methotrexate, flavopiridol, and anthracyclines [ 1 ]. (hindawi.com)
  • Panobinostat has also been found to significantly increase in vitro the survival of motor neuron (SMN) protein levels in cells of patients suffering from spinal muscular atrophy. (wikipedia.org)
  • A 2015 study suggested Panobinostat was effective in preventing diffuse intrinsic pontine glioma cell growth in vitro and in vivo, identifying it as a potential drug candidate. (wikipedia.org)
  • The publication of two seminal research papers in 1998 outlining the isolation of these cells provided an in vitro method by which researchers may characterise PaSCs in both health and pathology. (wikipedia.org)
  • malignant
  • Glucose depletion is often observed in malignant tumors due to insufficient blood supply in the core of solid tumors [ 5 ] and increased rate of glycolysis [ 6 ]. (hindawi.com)
  • As of August 2012[update], it is being tested against Hodgkin's Lymphoma, cutaneous T cell lymphoma (CTCL) and other types of malignant disease in Phase III clinical trials, against myelodysplastic syndromes, breast cancer and prostate cancer in Phase II trials, and against chronic myelomonocytic leukemia (CMML) in a Phase I trial. (wikipedia.org)
  • metastasis
  • Poly-α2,8-sialic acid (PSA) has been implicated in numerous normal and pathological processes, including development, neuronal plasticity, and tumor metastasis. (sciencemag.org)
  • Recent advances have indicated that the tumor microenvironment is critically important for cancer initiation, progression, metastasis, and drug resistance, thus providing opportunities for therapeutic intervention. (hindawi.com)
  • endothelial
  • INTRODUCTION: Vascuologenesis is the de novo establishment of blood vessels and vascular networks from mesoderm-derived endothelial cell precursors (angioblasts). (biomedsearch.com)
  • Recently a novel mechanism, by which some genetically deregulated and aggressive tumour cells generate "micro-vascular" channels without the participation of endothelial cells and independent of angiogenesis, has been proposed. (biomedsearch.com)
  • The endothelial growth factor antibodies used did not inhibit or stimulate cell growth when compared to control but did discourage vascular mimicry. (biomedsearch.com)
  • Its existence also suggests a further process that of the development of tumour mosaic vessels as the neo-vasculature integrates with the existing endothelial lined systems. (biomedsearch.com)
  • These soluble mediators could be secreted either by the tumor cells themselves or by any of the various cellular components of the tumor microenvironment, including cancer-associated fibroblasts, immune cells, and endothelial cells, among others ( 4, 5 ). (aacrjournals.org)
  • The potential sources of these activating factors include platelets, macrophages, pancreatic acinar cells and endothelial cells in inflamed pancreas. (wikipedia.org)
  • tissues
  • For Western blotting, tumor tissues from mice were collected in radioimmunoprecipitation (RIPA) buffer supplemented with a cocktail of protease inhibitors and Serine/Threonine and Tyrosine phosphatase inhibitors (Thermo Fisher Scientific). (aacrjournals.org)
  • For example, during gastrulation, clumps of stem cells switch off their cell-to-cell adhesion, become migratory, and take up new positions within an embryo where they again activate specific cell adhesion proteins and form new tissues and organs. (wikipedia.org)
  • Morphogenesis also involves changes in the cellular structure or how cells interact in tissues. (wikipedia.org)
  • The success of BNCT is dependent upon the selective delivery of sufficient amounts of 10B to the tumor with only small amounts localized in the surrounding normal tissues. (wikipedia.org)
  • survival
  • It is also believed to be a survival factor for cancer stem cells to drive tumor growth. (hindawi.com)
  • In particular, tumor-derived soluble factors have been shown to function in a paracrine fashion to reprogram the normal stroma to a tumorigenic stroma ( 6 ), as well as to work in an autocrine way promoting tumor growth, survival, and acquisition of metastatic potential ( 7 ). (aacrjournals.org)
  • bladder
  • Similar to our previous observations in human bladder cancer cells, Boswellia sacra essential oil induces breast cancer cell-specific cytotoxicity. (biomedcentral.com)
  • This gene is located within chromosome 9 (9q32-33), a chromosomal region that frequently shows loss of heterozygosity in transitional cell carcinoma of the bladder. (wikipedia.org)
  • grown
  • Furthermore, fibroblast overgrowth is a constant concern whenever a mixed population of cells is grown in serum-containing medium. (docplayer.net)
  • The cells are grown in a modified CMRL-1415 medium supplemented with 10% fetal calf serum. (aacrjournals.org)
  • Almost all known conventional cytotoxic anticancer drugs are less effective in killing tumor cells grown as multicellular spheroids than in killing tumor cells grown as monolayer cell cultures. (aacrjournals.org)
  • Taken together, the results indicate that unlike most other known anticancer cytotoxic drugs, PS-341 appears to be as effective in killing tumor cells grown in the form of multicell spheroids as in killing tumor cells grown in monolayer cell culture. (aacrjournals.org)
  • mediates
  • Furthermore, these results highlight the importance of pathologically relevant tissue culture models to study the complex physical and chemical processes by which the cancer microenvironment mediates new vessel formation. (sigmaaldrich.com)
  • The activation of P2 receptors induces intracellular calcium signalling which mediates the fibrogenic function of activated stellate cells. (wikipedia.org)
  • lung
  • 1 1 Growth of Human Lung Tumor Cells in Culture Reen Wu Center for Comparative Respiratory Biology and Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California, 1. (docplayer.net)
  • Introductory Review Cell Culture and Lung Cancer Development Requirements for Growth Factors and Hormones for Lung Cancer Cells to Grow Preparation of Media and Reagents Preparation of HITES-Based Medium Preparation of C and C-Modified Media Safety Precautions Step-by-Step Protocols Tissue Handling and Shipping Protocol 1.1. (docplayer.net)
  • Processing of Lung Tumor Samples Cell Dissociation Protocol 1.2. (docplayer.net)
  • Harvesting Cells from Lung Tumor by Mechanical Spillout Protocol 1.3. (docplayer.net)
  • Disaggregation of Lung Tumor Tissue in Collagenase Cell Culture Conditions Protocol 1.4. (docplayer.net)
  • Cloning Cultures from Lung Carcinoma Characterization of Neoplastic and Differentiated Functions Variations Discussion Acknowledgments Sources of Materials References INTRODUCTORY REVIEW 1.1. (docplayer.net)
  • Cell Culture and Lung Cancer Development Lung cancer is the most frequent cause of cancer deaths in both men and women [Landis et al. (docplayer.net)
  • Like many other tumors, the development of lung cancer is a multistep process the nature of which has not been fully elucidated. (docplayer.net)
  • We, as well as other researchers, initially used a serumsupplemented medium to establish primary cultures of normal specimens and lung tumor specimens. (docplayer.net)
  • pancreatic
  • Panobinostat has been found to synergistically act with sirolimus to kill pancreatic cancer cells in the laboratory in a Mayo Clinic study. (wikipedia.org)
  • In the study, investigators found that this combination destroyed up to 65 percent of cultured pancreatic tumor cells. (wikipedia.org)
  • The finding is significant because the three cell lines studied were all resistant to the effects of chemotherapy - as are many pancreatic tumors. (wikipedia.org)
  • Pancreatic stellate cells (PaSCs) are classified as myofibroblast-like cells that are located in exocrine regions of the pancreas. (wikipedia.org)
  • While the pathogenesis of fibrosis remains elusive, the activation of stellate cells contribute to pancreatic fibrosis. (wikipedia.org)
  • resistant
  • All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)
  • ABCG2 is an efflux transporter commonly found to overexpress in multidrug resistant (MDR) cancer cells. (hindawi.com)
  • Her2 is found to form heterodimers with Igf1r in resistant primary tumor cell cultures, and stimulation with Igf2 leads to Her2 phosphorylation. (aacrjournals.org)
  • Hence, this compound has the potential to circumvent multicellular drug resistance and, as such, may show promising activity against solid tumors with low growth fractions in vivo , which are frequently intrinsically resistant to conventional cytotoxic anticancer drugs. (aacrjournals.org)
  • Group 1-cells were resistant to both Paclitaxel and Imatinib. (biomedcentral.com)
  • microenvironment
  • This study investigated the upregulation of ABCG2 by these adverse growth conditions within the tumor microenvironment. (hindawi.com)
  • A better understanding of ABCG2 regulation by the tumor microenvironment may help design novel strategies to improve treatment outcome. (hindawi.com)
  • They can be thought of as inhabiting within a complex milieu of normal cells, blood vessels, endogenous small molecules, and secreted factors, which collectively comprise the tumor microenvironment. (hindawi.com)
  • Although multiple studies have focused on elucidating the signaling events that initiate this phenotypic switch, there has been so far no characterization of the pattern of soluble mediators released by tumor cells undergoing EMT, and the potential impact that this phenotypic switch could have on the remodeling of the tumor microenvironment. (aacrjournals.org)
  • Altogether, our results emphasize the potential role of EMT in the modulation of the tumor microenvironment via secretion of multiple soluble mediators and suggest that IL-8 signaling blockade may provide a means of targeting mesenchymal-like, invasive tumor cells. (aacrjournals.org)
  • Interleukin 8 (IL-8, CXCL8), a proinflammatory CXC chemokine, constitutes an example of a soluble mediator released by tumor cells that simultaneously function in an autocrine and paracrine mode within the tumor microenvironment. (aacrjournals.org)
  • lines
  • Vascular mimicry in cultured head and neck tumour cell lines. (biomedsearch.com)
  • We present experimental evidence of vasculogenic mimicry in HNSCC cell lines and compare them with other tumours and a positive control vascular cell line. (biomedsearch.com)
  • Metastatic HNSCC cells lines were found to have vasculogenic properties similar to HUVEC cell lines when compared to cell lines from their corresponding primary tumour. (biomedsearch.com)
  • Other tumour cell lines also displayed this property. (biomedsearch.com)
  • The focus of this study was to examine the CTC-iChip and determine if the device could properly capture CTCs to aid the formation of cell lines to represent the genetic status of all existing tumor sites. (oncologynurseadvisor.com)
  • Cell lines with longevity were successfully established from 6 patients. (oncologynurseadvisor.com)
  • Additional samples from 3 of these patients were used to set up new cell lines to track the effectiveness of therapy via tumor change. (oncologynurseadvisor.com)
  • Circulating tumor cells captured with a microchip-based device developed at the Massachusetts General Hospital (MGH) Center for Engineering in Medicine and the MGH Cancer Center can be cultured to establish cell lines for genetic analysis and drug testing. (oncologynurseadvisor.com)
  • Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
  • Complementary studies using primary tumor samples and cell lines have shown similarly that the level of IGF signaling is increased in RMS ( 6, 7 ). (aacrjournals.org)
  • In a previous study, we have shown the role of the T-box transcription factor Brachyury in the induction of EMT and metastatic potential in various human cancer cell lines ( 15 ). (aacrjournals.org)
  • growth
  • RESULTS: There were observable differences in growth of the cells on laboratory plastic and collagen matrix. (biomedsearch.com)
  • The Her2 inhibitor lapatinib cooperates with NVP-AEW541 to reduce Igf1r phosphorylation and to inhibit cell growth even though lapatinib alone has little effect on growth. (aacrjournals.org)
  • We found, with one exception, that PS-341 showed equal or greater activity in spheroids than in the respective monolayer cell cultures, even in a prostate cancer spheroid model with a very low growth fraction. (aacrjournals.org)
  • The role of multiple growth factors, cytokines, and components of the extracellular matrix (ECM) in inducing EMT in epithelial tumor cells has been well documented and, for example, TGF-β, fibroblast growth factor (FGF), and TNF-α have all been implicated in the induction of EMT ( 10 , 12-14 ). (aacrjournals.org)
  • Anchorage-independent cell growth was evaluated by colony formation in soft agar. (biomedcentral.com)
  • The biological effects of the omega-6 fatty acids are largely produced during and after physical activity for the purpose of promoting growth and during the inflammatory cascade to halt cell damage and promote cell repair by their conversion to omega-6 eicosanoids that bind to diverse receptors found in every tissue of the body. (wikipedia.org)
  • but studies in laboratory mice have shown that nelfinavir is able to suppress the growth of tumors in these animals, which represents a promising lead towards testing this drug in humans as well. (wikipedia.org)
  • In biological specimens containing a growth medium, appearance over time of a detectable and rising heat flow signal is a simple general indicator of the presence of some type of replicating cells. (wikipedia.org)
  • Growth in culture and anchorage-independent growth were inhibited in SFRP1-expressing UMRC3 cells. (wikipedia.org)
  • vivo
  • The rationale is that if a drug demonstrates antitumor activity in a spheroid model or screen comparable to or greater than that seen in a conventional monolayer system, it could indicate more promising efficacy for the treatment of solid tumors in vivo . (aacrjournals.org)
  • protein
  • However, some tumor cells are able to repair this type of DNA damage, and therefore diminish the therapeutic efficacy of temozolomide, by expressing a protein O6-alkylguanine DNA alkyltransferase (AGT) encoded in humans by the O-6-methylguanine-DNA methyltransferase (MGMT) gene. (wikipedia.org)
  • aggressive
  • The objective of this study was to optimize conditions for preparing Boswellea sacra essential oil with the highest biological activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells. (biomedcentral.com)
  • angiogenesis
  • This has been termed "vasculogenic mimicry" and has implications beyond angiogenesis and adds another layer of complexity to the current concept for the generation of tumour micro-circulation. (biomedsearch.com)
  • Oxygen status and tissue dimensionality are critical determinants of tumor angiogenesis, a hallmark of cancer and an enduring target for therapeutic intervention. (sigmaaldrich.com)
  • embryonic development
  • As of early 2016, there are three families belonging to the MACPF superfamily: 1.C.12 - The Thiol-activated Cholesterol-dependent Cytolysin (CDC) Family 1.C.39 - The Membrane Attack Complex/Perforin (MACPF) Family 1.C.97 - The Pleurotolysin Pore-forming (Pleurotolysin) Family Proteins containing MACPF domains play key roles in vertebrate immunity, embryonic development, and neural-cell migration. (wikipedia.org)
  • The process controls the organized spatial distribution of cells during the embryonic development of an organism. (wikipedia.org)
  • During embryonic development, cells are restricted to different layers due to differential affinities. (wikipedia.org)
  • invasive
  • Anti-invasive and anti-multicellular tumor properties were evaluated by cellular network and spheroid formation models, respectively. (biomedcentral.com)
  • molecules
  • determined that Saos-2 cells "possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules. (wikipedia.org)
  • An important class of molecules involved in morphogenesis are transcription factor proteins that determine the fate of cells by interacting with DNA. (wikipedia.org)
  • At the end of this cascade are classes of molecules that control cellular behaviors such as cell migration, or, more generally, their properties, such as cell adhesion or cell contractility. (wikipedia.org)
  • One of the ways this can occur is when cells share the same cell-to-cell adhesion molecules. (wikipedia.org)
  • For instance, homotypic cell adhesion can maintain boundaries between groups of cells that have different adhesion molecules. (wikipedia.org)
  • Characterization
  • Besides their worldwide availability, some of the advantages for using Saos-2 cell line are that they have well-documented characterization data, the possibility to obtain large amounts of cells in short time, and the fact that Saos-2 cells can be fully differentiated in a manner that the osteoblastic cells naturally do. (wikipedia.org)
  • therapeutic
  • The ability to effectively culture CTCs and examine them for genetic changes can be used to find targetable mutations and discover which therapeutic drugs are most effective. (oncologynurseadvisor.com)
  • processes
  • IMC is sensitive enough to detect and follow either slow processes (reactions proceeding at a few % per year) in a few grams of material, or processes which generate minuscule amounts of heat (e.g. metabolism of a few thousand living cells). (wikipedia.org)
  • cytotoxic
  • In addition, SCLC tumors initially have much better response to cytotoxic therapies than do NSCLC. (docplayer.net)
  • PS-341 has been found to have good broad-spectrum cytotoxic activity in the 60-monolayer cell line National Cancer Institute screen. (aacrjournals.org)
  • Perforin is released by cytotoxic T cells and lyses virally infected and transformed cells. (wikipedia.org)
  • In addition, perforin permits delivery of cytotoxic proteases called granzymes that cause cell death. (wikipedia.org)
  • Perforin is stored in granules within cytotoxic T-cells and is responsible for killing virally infected and transformed cells. (wikipedia.org)
  • Secondly, perforin permits delivery of the cytotoxic granzymes A and B into target cells. (wikipedia.org)
  • vascular
  • It provides a feasible mechanism of early tumour vascular supply which can co-exist and incorporate with later angiogenic mechanisms. (biomedsearch.com)
  • patients
  • Patients whose tumors express resistance to chemotherapeutic drugs tend to do so in one of two ways, either intrinsically or after showing an initial response. (aacrjournals.org)
  • selectively
  • After losing energy as they penetrate tissue, the neutrons are absorbed by the capture agent, which subsequently emits high-energy charged particles that can selectively kill tumor cells that have taken up sufficient quantities of 10B (Fig.1). (wikipedia.org)
  • refractory
  • This malignancy is a paradigm for refractory and incurable solid tumors at all ages because more than half of children with RMS at diagnosis have either regional lymph node or distant metastases ( 2 ). (aacrjournals.org)
  • As of 2016[update] panobinostat is being studied in a phase II trial for relapsed and refractory diffuse large B-cell lymphoma (DLBCL). (wikipedia.org)
  • primary
  • Subpopulations were isolated from MBCDF primary breast cancer cell culture by limiting dilution. (biomedcentral.com)
  • Saos-2 ("Sarcoma osteogenic") is a cell line derived from the primary osteosarcoma of an 11-year-old Caucasian girl in 1973 by Fogh et al. (wikipedia.org)
  • The village was primary designed for tourism to promote African culture and was generally ignored by the Sowetan locals, partly due to the unfamiliarity of the mythology that was being represented. (wikipedia.org)
  • represents
  • Despite all recent advances, intra-tumor heterogeneity is poorly understood, and it still represents the main challenge to judge how representative the analysis of a small biopsy is. (biomedcentral.com)
  • cancer
  • Researchers based at the Massachusetts General Hospital (MGH) Center for Engineering in Medicine and the MGH Cancer Center have created a microchip device, called the CTC-iChip, which can capture circulating tumor cells in order to track ongoing genetic mutation within a tumor. (oncologynurseadvisor.com)
  • Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
  • The cell type origin of these cancer cells is still unknown. (docplayer.net)
  • Cancer cells do not exist in isolation. (hindawi.com)
  • Several subpopulations were isolated from the MBCDF breast cancer cells that were divided into two groups according to their morphology. (biomedcentral.com)
  • partially reversing the morphological changes characteristic of breast cancer cells. (wikipedia.org)
  • February 23, 1914 - August 26, 1998), was an American physician and virologist whose research into viruses, their causes and treatment that led to his breakthrough insights into the connections between viruses and cancer, leading to new treatments, as well as his hypothesized oncogene, which was discovered to be a trigger for normal cells turning cancerous. (wikipedia.org)
  • Each component in itself is non-tumoricidal, but when combined together they are highly lethal to cancer cells. (wikipedia.org)
  • laboratory
  • After culturing and isolating the organism in laboratory mice, the pathogen they named Rickettsia akari was identified as the ultimate cause of the disease now called rickettsialpox. (wikipedia.org)