Tumor drugs drug screening assays antitumor. Medical search. Frequent questions

Cytoskeleton, Inc. offers a wide range of kits and products for drug screening, signal transduction and cytoskeletal research. (cytoskeleton.com)

Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
Moreover, the algorithm was able to distinguish cytotoxic vs. cytostatic drug effects and drug-resistant clones that will give rise to later relapse. (technologynetworks.com)
Additionally, the algorithm was able to distinguish between cytotoxic and cytostatic effects of drugs and drug-resistant clones that will cause subsequent relapse. (genericviabuy.com)
Despite the identification of tumor-specific somatic mutations, most cancer therapy still utilizes nonspecific, cytotoxic drugs. (broadinstitute.org)

Immunosuppressive agents encompassed a large number of drugs, mainly represented by anti-tumour necrosis factor (TNF)-α, anti-retroviral therapy (ARV), azathioprine (AZT), methotrexate (MTX), mycophenolate mofetil (MMF), non-steroidal anti-inflammatory drugs (NSAIDs), rituximab and steroids. (ersjournals.com)
These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)

A malignant epithelial tumor with a glandular organization. (lookformedical.com)
Malignant cystosarcoma phyllodes (MCSP) is a rare breast tumor. (ox.ac.uk)
The epithelial‑mesenchymal transition (EMT) serves vital roles in the angiogenesis, cell invasion and metastasis of various malignant tumors, including bladder cancer. (spandidos-publications.com)
Moreover, common targeted therapeutic drug formulations have weak stability in malignant tumor leukemia, and their application effects are limited. (frontiersin.org)
Leukemia is a malignant tumor of the hematopoietic system, mainly characterized by cell deoxyribonucleic acid mutations. (frontiersin.org)

MOS could be further integrated with deep-learning imaging analysis for rapid drug testing of small and heterogeneous clinical tumor biopsies. (technologynetworks.com)
MOS can be further integrated with deep learning image analysis for rapid drug testing of clinical biopsies of small and heterogeneous tumors. (genericviabuy.com)

Within natural diffusion limit of vascularized tissue, tumor-derived MOS allowed sufficient penetration by therapeutic immune T-cells such as CAR-T, enabling a novel T cell potency assay to assess tumor killing by the engineered T-cells. (technologynetworks.com)

It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. (lookformedical.com)

Using a mouse model of ependymoma-a chemoresistant brain tumor-we combined multicell high-throughput screening (HTS), kinome-wide binding assays, and in vivo efficacy studies, to identify potential treatments with predicted toxicity against neural stem cells (NSC). (nih.gov)
As documented in a recent publication in Stem Cell Reports , their MOS can be used as patient avatars for studies involving direct viral infection, immune cell penetration and high-throughput therapeutic drug screening, something that is not obtainable with conventional patient-derived models. (technologynetworks.com)
A small-molecule library enriched for FDA-approved drugs was screened with a high-throughput, ligation-mediated amplification assay with a fluorescent, bead-based detection. (broadinstitute.org)

Protein binding assays assesses the degree to which drugs attaches to proteins within the plasma/tissue. (td2inc.com)

FDA approved drugs not currently used to treat ependymoma were also identified that posses selective toxicity against ependymoma cells relative to normal NSCs both in vitro and in vivo, e.g., 5-fluorouracil. (nih.gov)
We studied the chemosensitivity of these two MCSP tumor xenografts to anticancer drugs in vivo. (ox.ac.uk)
In vitro drug metabolism studies, are a screening mechanism to characterize drug metabolites, elucidate their pathways, to help with further in vivo testing. (td2inc.com)
In contrast to results with 2D culture, observed mitotic arrest was relatively low, suggesting involvement of additional mechanisms in their antitumor effect in vivo . (nature.com)
Analysis of mitosis and antimitotic drug responses in tumors by in vivo microscopy and single-cell pharmacodynamics. (nature.com)
Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo . (nature.com)
The authors found that the peptide induces apoptotic effects on multiple cancer cells both in vitro and in vivo , inhibits tumor cell migration and invasion, and arrests cell cycle primarily in the G2M phase. (cytoskeleton.com)
BK011P) was an essential reagent in this study, complementing the in vivo results and in vitro cell culture data which demonstrated conclusively that the C36L1 peptide targets microtubules in exerting its anti-tumor effects. (cytoskeleton.com)
2015. A novel microtubule de-stabilizing complementarity-determining region C36L1 peptide displays anti-tumor activity against melanoma in vitro and in vivo . (cytoskeleton.com)

We applied our workflow to quantify cell-cycle effects of three antimitotic cancer drugs over 8 d in HT-1080 fibrosarcoma xenografts in living mice using a data set of 38,000 cells and compared the induced phenotypes. (nature.com)
Notably, IM-T9P1 triggered complete tumor regression in 39% (7/18) of MC38 tumor-bearing mice (compared with the 0% regression rate following PD-L1 monoclonal antibody treatment), and in 77% (30/39) of EMT6 tumor-bearing mice (compared with the 28% response rate following PD-L1 monoclonal antibody treatment). (izb-online.de)

The present study aimed to screen the sensitivity of bladder cancer to natural compounds by using six classic anti‑inflammatory and detoxifying herbs, including the ethanol extract of Paris polyphylla (PPE), Scutellaria barbata, Pulsatillae decoction, Dahuang Huanglian Xiexin decoction, Bazhengsan and Hedyotis diffusa combined with S. barbata, were used to treat bladder cancer cells in vitro. (spandidos-publications.com)

Studies in experimental incidence and multiplicity of tumours to Wilms tumour in humans - in the animals increase and the latency period de adult rat after perinatal exposure to a creases with increasing dose. (who.int)

We report pulmonary M . haemophilum infection in a woman who had been immunosuppressed by tumor necrosis factor-α antagonist (TNF-αA) (adalimumab) treatment for rheumatoid arthritis. (cdc.gov)

These results demonstrate the importance of DCs in overcoming ICI resistance and achieving effective and durable antitumor responses and, in many cases, antitumor immunity. (izb-online.de)
This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
This heterogeneity affects response to surgery and/or systemic therapy, and also facilitates inherent and acquired drug resistance. (cdc.gov)

Such a model would be highly useful in investigating tumor responsiveness and in developing anti-tumor immune cell therapies. (technologynetworks.com)
Such a model would be very useful in the investigation of tumor response and in the development of anti-tumor immune cell therapies. (genericviabuy.com)
Secarna Pharmaceuticals, an IZB-based biopharmaceutical company focusing on the discovery and development of next-generation antisense oligonucleotide (ASO) therapies to address challenging or previously undruggable targets via its LNAplus™ platform, announced the publication of striking new preclinical data demonstrating that bimodal ASOs can enable efficacious long-term antitumor immunity in tumor models compared to current immune checkpoint inhibitors. (izb-online.de)

However, studies have reported that some of most commonly used synthetic antioxidants such as Tert-butyl hydroxy anisole (BHA), tert-butyl hydroxytoluene (BHT) are tumor promoters and can induce impairment in blood clotting [ 5 ], therefore research has been directed towards plant derived natural antioxidants. (biomedcentral.com)
Figure 6: Antimitotic drugs induce different mitotic and interphase phenotypes. (nature.com)

They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. (lookformedical.com)

A cell line derived from cultured tumor cells. (lookformedical.com)
It combines image analysis and machine learning methods for automated 3D segmentation and cell-cycle state identification of individual cell-nuclei with widely varying morphologies embedded in complex tumor environments. (nature.com)
Figure 4: Quantitative analysis of drug response to paclitaxel treatment at a single-cell level. (nature.com)
Functional T-cell assays are exposed to bias at several levels (manufacturing, pre-analytical, analytical and immunological) [ 2 ] and, unfortunately, the lack of a gold standard precludes higher diagnostic accuracy. (ersjournals.com)
Collectively, these data show that IM-T9P1 leads to long-lasting, adaptive antitumor immunity that can be further improved when combined with T cell-targeting immune checkpoint inhibitors and can have efficacy against large, established tumors in preclinical tumor models. (izb-online.de)
The activity of IM-T9P1 was tested in cancer cell lines and tumor models. (izb-online.de)
Treatment with IM-T9P1 resulted in the sustained activation of a specific type of tumor infiltrating cell, dendritic cells 3 (DC3s), which can promote antitumor immunity by supporting T cells with survival signals and enabling them to perform their effector functions. (izb-online.de)
ARA-C reduced EWS/FLI protein abundance and accordingly diminished cell viability and transformation and abrogated tumor growth in a xenograft model. (broadinstitute.org)
We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)
Autophagy provides energy to tumor cells for survival and metabolic reprogramming, in order to accommodate rapid cell growth and proliferation ( 8 ). (spandidos-publications.com)
In addition, Beclin 1 gene deletion results in tumor cell apoptosis, specifically in hypoxic regions ( 12 ). (spandidos-publications.com)

To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
Screening tools for the evaluation of a drug's ability to cross various biological membrane systems in a microplate format. (td2inc.com)
Its drug release characteristics perform well in photothermal therapy, indicating that black phosphorus nano drugs can improve the biological safety and applicability of drugs in new tumor targeted therapy. (frontiersin.org)

These drugs inhibit immune-suppressive signals and reinstate cancer immunosurveillance. (izb-online.de)
Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)

Chronic inflammation increases the risk of cancer and contributes to tumor development through the induction of oncogenic mutations, enhanced angiogenesis and early tumor promotion ( 7 ). (spandidos-publications.com)

A lesion with cytological characteristics associated with invasive carcinoma but the tumor cells are confined to the epithelium of origin, without invasion of the basement membrane. (lookformedical.com)
The two-dimensional nano drug carrier has high safety and effectiveness in drug delivery, and its targeted treatment effect on acute T lymphoblastic leukemia cells is obvious. (frontiersin.org)
Targeted therapy uses targeted drugs to maintain a high concentration of the drug in the tumor area, improving the killing efficiency of cancer cells while avoiding damage to other normal cells. (frontiersin.org)
Drug delivery systems seem to be a new method for improving drug targeting and release into cells, bringing new opportunities for improving drug efficiency and avoiding potential side effects. (frontiersin.org)
Increasing amounts of evidence indicate that autophagy is induced by a number of stressors in tumor cells, such as starvation, growth factor deprivation, hypoxia, damage stimulation and therapeutic drugs, and is an important survival mechanism in response to cellular stress ( 9 ). (spandidos-publications.com)
Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)

This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)

The presence of tumor-specific mutations in the cancer genome represents a potential opportunity for pharmacologic intervention to therapeutic benefit. (broadinstitute.org)

Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)

Corresponding cultures yielded a strain identified as M . haemophilum at the Netherlands National Institute for Public Health and the Environment (RIVM) by using the Inno-LiPA Mycobacteria v2 reverse line blot assay (Innogenetics, Ghent, Belgium). (cdc.gov)
Using cDNA microarray screens and northern blot analysis, a previous study showed that Beclin 1 is upregulated in liver cancer tissues ( 11 ). (spandidos-publications.com)

Autophagy, an evolutionarily conserved catabolic process, has a crucial role in tumor initiation and progression. (spandidos-publications.com)

Traditional Chinese medicine provides the material basis and molecular structure framework for the screening of antitumor drugs. (spandidos-publications.com)

Tumors or cancer of the MOUTH. (lookformedical.com)
Tumors or cancer of the SKIN . (lookformedical.com)
Tumors or cancer of the LUNG. (lookformedical.com)
Tumors or cancer of the LIVER. (lookformedical.com)
Tumors or cancer of the UTERINE CERVIX. (lookformedical.com)
The treatment options for bladder cancer include radiation alone, chemotherapy alone, combination treatment with radiation and chemotherapy or surgery, and the selected treatment is dependent on the age of the patient, the aggressiveness of the disease and drug effectiveness ( 6 ). (spandidos-publications.com)
There is a trend, although not statistically significant, toward younger age at cancer diagnosis, more likely to participate in cancer screening programs (71.8% vs. 45.5%), and more likely to have daughters (63.3% vs. 37.5%) in the group that accepted the test. (cdc.gov)

Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
We hypothesize that screening small-molecule libraries highly enriched for FDA-approved drugs will provide a more rapid path to clinical application. (broadinstitute.org)
We demonstrate that a gene expression-based approach to small-molecule library screening can identify, for rapid clinical testing, candidate drugs that modulate previously intractable targets. (broadinstitute.org)

Unfortunately, many classes of oncoproteins (e.g., transcription factors) are not amenable to conventional small-molecule screening. (broadinstitute.org)

This CDR exhibits both anti-tumor activity and functions as a microtubule destabilizing molecule. (cytoskeleton.com)
The clandestine chemist has to switch their method of production according to the availability of different starting materials, thus a number of synthetic routes are available to a given drug molecule. (soci.org)
The combined downregulation of PD-L1 expression in a single-molecule approach resulted in exceptional antitumor activity in different tumor models. (izb-online.de)
Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma. (broadinstitute.org)
Previously, this target has been intractable to modulation with traditional small-molecule library screening approaches. (broadinstitute.org)
Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)

examined the mechanism of action underlying the anti-tumor effects of the complementarity-determining region (CDR) C36L1 synthetic peptide derived from the V L CDR1 of the C36 Fab fragment of the anti-vaccinia immunoglobulin. (cytoskeleton.com)
Its low toxicity, high efficiency, and controllable drug release advantages make it have good application effects. (frontiersin.org)
Side effects of concern are infectious diseases, which prompt the need for screening for latent mycobacterial infection before commencing treatment ( 7 ). (cdc.gov)

The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
The Drug Repurposing Hub is one of the most comprehensive and up-to-date biologically annotated collections of FDA-approved compounds in the world. (broadinstitute.org)

Notably, the tuberculin skin test result was negative at screening before she began the TNF-αA treatment, but was now positive (20 mm), suggesting mycobacterial infection. (cdc.gov)

antigens, methods, and interpretation criteria for these assays differ from those for IGRAs approved previously by FDA. (cdc.gov)

Here, the authors investigated whether its microtubule-destabilizing effect is responsible for its anti-tumor activity. (cytoskeleton.com)

therefore, uncovering the underlying mechanism of metastasis and screening for antimetastatic drugs is urgently needed. (spandidos-publications.com)

Therefore, based on the characteristics of black phosphorus two-dimensional nanomedicine, experimental designs were conducted on its nanosheet preparation, polyethylene glycol modification, and anti-tumor drug loading. (frontiersin.org)
Experimental analysis was also conducted on the characterization ability, drug release, and targeted therapy of nanomedicine. (frontiersin.org)

This one-day conference held on 1 July 2008, and sponsored by SCI, the RSC, and ICI brought together scientists from the Republic of Ireland and from Northern Ireland who have varied interests in drug discovery and development and a common interest in synthesis. (soci.org)
Professor Maguire underlined the central importance of organic synthesis to the field of chemical biology and the interfaces with drug development and process development. (soci.org)
The development of bioinformatics technology has enabled nanomedicine to play a significant role in drug delivery systems. (frontiersin.org)
Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)

NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)

Patients can usually be treated with nonsteroidal anti-inflammatory drugs (NSAIDs) alone. (msdmanuals.com)

This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)

Keating's programme aims to elucidate full impurity profiles for drugs in relation to the full synthetic pathway employed. (soci.org)

The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)

TD2 has validated this assay using the Rapid Equilibrium Device and analysis done by LC-MS. (td2inc.com)

Dr. Shen and his team continue to refine and improve upon the MOS technology and to spotlight its versatility, not only as a physiological model for screening potential personalized treatments, but for disease studies and a variety of other applications as well," said Ali Khademhosseini, Ph.D., TIBI's Director and CEO. (technologynetworks.com)
Dr. Shen and his team continue to refine and improve the MOS technology and demonstrate its versatility, not only as a physiological model for screening potential personalized treatments, but also for disease studies and a variety of applications of others," said Ali. (genericviabuy.com)

Hence, relatively straightforward analytical detection of this pyridine can instantly link, or not, a particular batch of drug to a particular clandestine production facility. (soci.org)

Although the EWS/FLI oncoprotein, present in the vast majority of Ewing tumors, was characterized over ten years ago, it has never been exploited as a target of therapy. (broadinstitute.org)

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