AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and Equipment
PodocytesKidney GlomerulusPuromycin AminonucleosideGlomerulosclerosis, Focal SegmentalNephrosisBowman CapsuleGlomerular Basement MembraneAIDS-Associated NephropathyProteinuriaGlomerular Filtration BarrierWT1 ProteinsNephrotic SyndromeKidneyMembrane ProteinsDiabetic NephropathiesGlomerulonephritisCyclin IGlomerulonephritis, MembranousCells, CulturedNephrosis, LipoidDenys-Drash SyndromeSialoglycoproteinsAlbuminuriaMesangial CellsKidney DiseasesMice, TransgenicCell Line, TransformedGlomerular MesangiumMicrofilament ProteinsZonula Occludens-1 ProteinActininMicroscopy, ImmunoelectronActinsTRPC Cation ChannelsEpithelial CellsRNA, MessengerGlomerulonephritis, IGA
EpithelialProximal tubular cellsEndothelial cellsCellsRenal tubular epitheliumImmunofluorescenceProteinuriaUrinaryInterstitialSynaptopodinInjuryOverlyingProteinDistalAtrophyNucleiAutophagyNephrinHighly specializedTubulesAccumulationLesionsBasementAlbuminuriaGlomerulosclerosisTubulointerstitial kidney diseaseFoot processesPathophysiologyFiltrationHomeostasisProcessesProgressionVascularPrimaryInflammatoryMiceGeneFunctionsFunctionRoleProteins
Epithelial10
  • In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
  • In this study, we explored the role of Plk1 in the classical kidney fibrosis model, UUO, and cultured fibroblast and renal tubular epithelial cells by using specific Plk1 inhibitors, genetic silencing, and heterozygous global knockout mice. (nature.com)
  • Renal filtration depends on the integrity of epithelial and endothelial monolayers in the tubular and glomerular apparatus. (biomedcentral.com)
  • Thus, the present study elucidated a novel mechanism by which both BNIP3-mediated and PINK1-PARK2-mediated mitophagy protects against cisplatin-induced renal tubular epithelial cell ferroptosis through the ROS/HO1/GPX4 axis. (ijbs.com)
  • The mechanism of cisplatin-induced acute kidney injury includes oxidative stress, mitochondrial dysfunction, and endoplasmic reticulum stress, which result in apoptosis, necrosis and ferroptosis of renal tubular epithelial cells, causing rapid loss of kidney function [ 8 - 11 ]. (ijbs.com)
  • LOXL2 protein expression was detected in glomerular capillary loops and tubular epithelial cells in human and mouse kidneys. (omicsdi.org)
  • Immunostaining of the renal specimen of IgAN patients revealed that TFF3 is located in the renal tubular epithelial cells. (omicsdi.org)
  • In the renal glomeruli, VEGFA is mainly expressed in and secreted from podocytes and tubular epithelial cells, which induces renal injury through binding to the receptor VEGFR, mainly VEGFR2, which is expressed on the surface of endothelial cells [ 8 ]. (biomedcentral.com)
  • Early in 2009 Nate posted on work suggesting that renal tubular epithelial cells had the ability to repopulate the renal tubular epithelium after ischemia reperfusion injury. (renalfellow.org)
  • A. Collapsing glomerulopathy with collapse of the glomerular capillary loops and prominence of the overlying podocytes and/or parietal epithelial cells forming a radially-oriented proliferation around the collapsed glomerular tuft. (ajkdblog.org)
Proximal tubular cells3
  • We were probably the first to describe the electron microscopy finding of the SARS-CoV-2 virus in kidney proximal tubular cells and also in podocytes of the glomerulus. (medscape.com)
  • This result was supported by western blot analysis, which demonstrated that LOXL2 protein expression is present in cultured human podocytes and HK?2 human proximal tubular cells. (omicsdi.org)
  • Distinct Requirements for Vacuolar Protein Sorting 34 Downstream Effector Phosphatidylinositol 3-Phosphate 5-Kinase in Podocytes Versus Proximal Tubular Cells. (umich.edu)
Endothelial cells4
  • The renal corpuscle filtration barrier is composed of: the fenestrated endothelium of glomerular capillaries, the fused basal lamina of endothelial cells and podocytes, and the filtration slits of the podocytes. (wikipedia.org)
  • Other reports also show that DN is highly associated with accelerated aging in various types of cells such as tubular cells, podocytes, mesangial cells, and endothelial cells [ 21 - 23 ]. (hindawi.com)
  • Through in vivo and in vitro experiments, we found that it promotes the proliferation, migration, and tubular formation of brain microvascular endothelial cells (BMECs). (cbinsights.com)
  • Proteinuria occurs because of changes to capillary endothelial cells, the glomerular basement membrane (GBM), or podocytes, which normally filter serum protein selectively by size and charge. (msdmanuals.com)
Cells16
  • The common pathological feature of CKD is the loss of tubular cells and progressive interstitial fibrosis. (nature.com)
  • Post-mitotic cells, like neurons in the central nervous system, and podocytes or tubular cells in the kidney, are particularly susceptible to MC. (intechopen.com)
  • Its administration in a mouse AKI model showed a diminished capacity of healing and the specific deletion of Mdm2 in both podocytes and tubular cells induced p53-overexpression dependent cell death (podoptosis). (uni-muenchen.de)
  • For this reason we hypothesized that the administration of Nutlin-3a to healthy mice damages renal function by promoting podoptosis in podocytes and tubular cells. (uni-muenchen.de)
  • Consistent with the hypothesis, we observed that stimulation with Nutlin-3a increased cell death in cultured mouse podocytes and tubular cells. (uni-muenchen.de)
  • Tubular and glomerular cells represent target cells of hantavirus infection. (biomedcentral.com)
  • Hantaviral N protein was detected in tubular and glomerular cells and cell-to-cell contact structures were changed in biopsy samples of patients with serologically confirmed PUUV infections [ 3 ]. (biomedcentral.com)
  • When filtration cells (podocytes) in the kidney's glomeruli become damaged due to either immune complexes in the blood (called glomerulonephritis), or due to dense deposits of hard protein (amyloid), abnormal accumulation of which is called amyloidosis, degeneration of the kidney's tubular system occurs. (petmd.com)
  • We propose the outline of such a mechanism based on following aspects: (1) The glomerulus is a complex structure, the stability of which depends on the cooperative function of the basement membrane, mesangial cells and podocytes, counteracting the distending forces originating from the high glomerular hydrostatic pressures. (scienceopen.com)
  • when podocytes are lost for any reason they cannot be replaced by new cells. (scienceopen.com)
  • For the first time to our knowledge, we demonstrate that CRELD2 can serve as a sensitive urinary biomarker for detecting ER stress in podocytes or renal tubular cells in murine models of podocyte ER stress-induced nephrotic syndrome and tunicamycin- or ischemia-reperfusion-induced acute kidney injury (AKI), respectively. (jci.org)
  • Kidney tubuloids derived from adult stem cells offer the advantage of long-term culture and expansion, but they include only tubular structures and lack glomerular components. (j-organoid.org)
  • Fatty acid-binding protein 3 (FABP3) located in renal mesangial and distal tubular cells, and had been shown to be a sensitive marker of renal injury, potentially be a mediator in pathogenesis of chronic kidney disease (CKD). (medsci.org)
  • However, paradoxically, the expression levels of YAP, TAZ and their target genes were massively increased after the deletion and YAP mainly activated in tubular cells. (trainckdis.eu)
  • Identify the targeted genetic networks responsible of the renal lesions in tubular cells expressing or not YAP and TAZ. (trainckdis.eu)
  • Determine the molecular mechanisms by which YAP/TAZ shape tubular cells would help to understand the pathophysiology of tubular epithelia maintenance. (trainckdis.eu)
Renal tubular epithelium1
  • Disease manifestations originate specifically in glomerular podocytes, as renal disease is seen in Podocin/cre,Pdss2(loxP/loxP) knockout mice but not in conditional knockouts targeted to renal tubular epithelium, monocytes, or hepatocytes. (nih.gov)
Immunofluorescence1
  • Glomerular LOXL2 was localized to the cytoplasm of podocytes, as determined by double immunofluorescence microscopy using a podocyte marker (synaptopodin). (omicsdi.org)
Proteinuria7
  • Microhematuria and subsequently proteinuria are hallmarks of kidney involvement, which are due to primary basement membrane alterations that mainly cause endothelial thrombosis and podocyte contraction and ulterior irreversible detachment. (hindawi.com)
  • The present case illustrates the reduction rate of urinary podocyte loss and proteinuria after amiloride administration and suggests the molecular pathways involved in Alport renal disease. (hindawi.com)
  • Urinary podocyte loss is a silent phenomenon that precedes proteinuria in glomerular diseases [ 3 , 4 ]. (hindawi.com)
  • Tubular proteinuria is a result of tubulointersitial disease affecting the proximal renal tubules and interstitium. (medscape.com)
  • Most cases of proteinuria can be classified as tubular, overflow, or glomerular. (medscape.com)
  • Tubular proteinuria occurs most commonly in disease processes affecting the tubulo-interstitial component of the kidney. (medscape.com)
  • Endothelin also directly acts on podocytes, where it can promote slit diaphragm and cytoskeletal disruption, podocyte loss, and proteinuria-actions that characterize FSGS. (medtelligence.net)
Urinary2
  • Most importantly, urinary CRELD2 elevation occurs in patients with autosomal dominant tubulointerstitial kidney disease caused by UMOD mutations, a prototypical tubular ER stress disease. (jci.org)
  • Fiseha T, Tamir Z. Urinary Markers of Tubular Injury in Early Diabetic Nephropathy. (viamedica.pl)
Interstitial2
  • However, the role of Plk1 in kidney tubular interstitial fibrosis has not been reported. (nature.com)
  • The aging kidneys undergo a wide range of macrostructural changes, such as decreased cortical volume, increased surface roughness, and augmented numbers and sizes of cysts [ 11 ], which correspond to the typical microstructural features of glomerulosclerosis, tubular atrophy, interstitial fibrosis, and nephron loss [ 12 ]. (hindawi.com)
Synaptopodin1
  • Experiments were performed with non-proliferating, differentiated podocytes expressing synaptopodin as podocyte-specific marker of differentiation. (biomedcentral.com)
Injury6
  • Moreover, increased macrophage infiltration was observed together with upregulation of inflammatory and tubular injury markers and augmented blood urea nitrogen levels. (uni-muenchen.de)
  • To evaluate the SPT's effectiveness, we utilized a mouse proximal tubular injury model, where traditional renal pathologists face challenges in identifying glomerular changes and podocyte morphology alterations. (vumc.org)
  • Shp2 Associates with and Enhances Nephrin Tyrosine Phosphorylation and Is Necessary for Foot Process Spreading in Mouse Models of Podocyte Injury. (umich.edu)
  • In addition to tubular injury, kidney biopsy case series have revealed diverse injury patterns which can be loosely generalized into podocytopathies, thrombotic microangiopathy (TMA)/acute endothelial injury, and sequelae of immune activation. (ajkdblog.org)
  • Kidney biopsy demonstrated collapsing glomerulopathy with ultrastructural features of acute endothelial injury, as well as acute tubular injury. (ajkdblog.org)
  • Finally, acute tubular injury is relatively common. (ajkdblog.org)
Overlying1
  • Overlying podocyte foot processes are diffusely effaced (transmission electron microscopy, original magnification 5870x). (ajkdblog.org)
Protein4
  • The impaired motility depends on viral replication and transfection of podocytes with N protein of PUUV or HTNV reveals that the expression of N protein alone is sufficient to deteriorate podocyte function. (biomedcentral.com)
  • NPHS2 is almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli, and encodes a new integral membrane protein, podocin, belonging to the stomatin protein family. (scienceopen.com)
  • The M-type phospholipase A2 receptor (PLA2R), thrombospondin type 1 domain-containing 7A (TSHD7A), and neural epidermal growth factor-like 1 protein (NELL-1) are major autoantigens against podocyte antigens in idiopathic membranous nephropathy (IMN) [ 1 ]. (biomedcentral.com)
  • Protein resorption droplets are apparent in podocytes (Jones 400x). (ajkdblog.org)
Distal1
  • Nevertheless, YAP and TAZ function appears to be cell-and context-dependent and in the kidney, YAP and TAZ are constitutively expressed in podocytes and distal tubules. (trainckdis.eu)
Atrophy1
  • Arrowheads indicate tbular dilation, tubular atrophy with tubular basement membrane thickening. (jci.org)
Nuclei1
  • The SPT successfully extracted and analyzed morphological and textural features from podocyte nuclei, revealing a multitude of podocyte morphomic features through statistical analysis. (vumc.org)
Autophagy3
  • Podocyte conditional knockin (cKI) or systemic overexpression of circ -0,000,953 alleviated albuminuria and restored macroautophagy/autophagy in kidney of diabetic mice. (bvsalud.org)
  • Silencing of Mir665-3p or overexpression of Atg4b recovered podocyte autophagy both in vitro and in vivo. (bvsalud.org)
  • In conclusion, this study revealed that circ -0,000,953 regulates podocyte autophagy by targeting Mir665-3p-Atg4b in DN. (bvsalud.org)
Nephrin1
  • Proximal tubule dysfunction is associated with podocyte damage biomarkers nephrin and vascular endothelial growth factor in type 2 diabetes mellitus patients: a cross-sectional study. (viamedica.pl)
Highly specialized1
  • Additionally, the researchers uncovered that this class of cilia-associated genes is in fact vitally important for the function of kidney podocytes, a highly specialized cell type that paradoxically does not carry cilia. (mpg.de)
Tubules2
  • In tubules, changes including tubular basophilia were observed radially, mainly around interlobular arteries with lesions. (go.jp)
  • Furthermore, ACE-2 is highly expressed in renal proximal tubules, where SARS-CoV-2 particles were detected postmortem in podocytes of COVID-19 patients, suggesting that the kidneys could also be one of the targets of SARS-CoV-2 ( 6 , 7 ). (frontiersin.org)
Accumulation2
  • In glomeruli, changes including hyaline droplet accumulation in podocytes, which was accompanied by increased expression of desmin, were observed. (go.jp)
  • It is histologically defined by renal glomerular hypertrophy, expansion of mesangial and tubular compartments, accumulation of mesangial extracellular matrix proteins, and podocytopenia associated with foot process effacement. (emjreviews.com)
Lesions1
  • Unexpectedly, the mouse model KO for both Yap and Taz developed severe tubular lesions and renal failure. (trainckdis.eu)
Basement1
Albuminuria1
  • It seems incredible now that it was only in 1998 that it was conclusively proven that the podocyte plays a vital role in the prevention of albuminuria. (renalfellow.org)
Glomerulosclerosis1
  • In the central nervous system, MC has been claimed as the cause of neuronal death in many neurologic disorders, while MC in podocytes and tubular death is connected with the development of progressive glomerulosclerosis. (intechopen.com)
Tubulointerstitial kidney disease1
  • and mutations in uromodulin (UMOD) have been reported to trigger tubular ER stress in autosomal dominant tubulointerstitial kidney disease. (cellcards.org)
Foot processes1
  • Podocytes have foot processes, pedicles, that wrap around glomerular capillaries. (wikipedia.org)
Pathophysiology1
  • Elucidate the role of mechanical stresses in tubular YAP/TAZ activation should help to better understand the importance of mechanical forces in renal pathophysiology. (trainckdis.eu)
Filtration1
Homeostasis2
  • Class III PI 3-kinase is the main source of PtdIns3P substrate and membrane recruitment signal for PIKfyve constitutive function in podocyte endomembrane homeostasis. (umich.edu)
  • The aim of the project is thus to define the role of YAP and TAZ focusing on the tubular homeostasis during CKD progression. (trainckdis.eu)
Processes1
  • The lamina rara externa is adjacent to the podocyte processes. (wikipedia.org)
Progression1
  • Progression of glomerular diseases: is the podocyte the culprit? (scienceopen.com)
Vascular2
  • There are two poles in the renal corpuscle, a vascular pole and a tubular pole. (wikipedia.org)
  • The tubular pole is at the other end opposite to the vascular pole. (wikipedia.org)
Primary2
  • Primary podocytes (Lonza) were maintained in RPMI medium (Capricorn) supplemented with 10% fetal calf serum (FCS). (biomedcentral.com)
  • Microscopic images of primary podocytes 5 min after removal of the depolymerisation reagent. (mpg.de)
Inflammatory1
  • Conclusions: Metformin could present its podocyte-protective capacity in type 2 diabetics and the underlying mechanisms may be partly attributed to its effects in suppressing MIF-CD74 axis mediated inflammatory cascade response. (viamedica.pl)
Mice1
  • Notably, these pathomic features differentiated podocyte phenotypes between disease-affected and healthy mice, indicating its diagnostic potential. (vumc.org)
Gene2
  • Using the Dignet dynamic gene interaction literature mining program, we have identified over 700 podocyte-associated gene-gene interactions . (cellcards.org)
  • Furthermore, we have developed a SciMinor and BioBERT-based literature mining program, and used it to identify more podocyte-associated gene interactions from the PubMed literature. (cellcards.org)
Functions1
  • These results further our understanding of the biologic mechanisms of kidney function by identifying loci that potentially influence nephrogenesis, podocyte function, angiogenesis, solute transport and metabolic functions of the kidney. (bioseek.eu)
Function1
  • Normal kidney filter function depends on the integrity of GBM and of tubular and glomerular monolayers. (biomedcentral.com)
Role1
  • Podocytes play a pivotal role in maintaining renal health. (vumc.org)
Proteins2
  • The impact of hantaviral nucleocapsid proteins (N proteins) on motility was examined by transfection of podocytes. (biomedcentral.com)
  • Podocytes in CG characteristically lose expression of WT-1 and increase expression of proteins involved in cell division (e.g. (basicmedicalkey.com)