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  • enzyme
  • However, certain cytoplasmic reductive enzymes that use the redox chemistry of cysteine in their active sites do form disulfide bonds as part of their catalytic cycles, but these bonds are subsequently reduced to regenerate active enzyme. (genetics.org)
  • They showed that when bovine pancreatic ribonuclease, which contains four disulfide bonds, was reduced and denatured, it could reassemble into its active structure in the test tube in the presence of oxygen and in the absence of any enzyme catalysts. (genetics.org)
  • They did not see the necessity of looking for an enzyme that catalyzes disulfide bond formation itself. (genetics.org)
  • TrxB
  • Importantly, this study has demonstrated a role in the maintenance of S. aureus redox balance beyond the usual cellular functions of TrxB and TrxA. (sun.ac.za)
  • Hierdie studie het twee hoofdoelwitte gehad: die funksionele karakterisering van die MerA en YpdA flavoproteïene, en die assessering van substraat-promiskuïteit van die TrxB en TrxA proteïene. (sun.ac.za)
  • glutathione
  • There are four enzymes that provide the bulk of protection against deleterious reactions involving active oxygen in bacteria: SODs (superoxide dismutases encoded by sodA and sodB), catalases (katE and katG), glutathione synthetase (gshAB) and glutathione reductase (gor). (wikipedia.org)
  • vivo
  • Nevertheless, the kinetics of disulfide bond formation in ribonuclease in these experiments was very slow, incommensurate with the rapid kinetics that we now know occurs in vivo . (genetics.org)
  • activity
  • Although DsbA displays no proofreading activity for repair of wrongly paired disulfides, DsbC, DsbE and DsbG have been found to demonstrate proofreading activity. (wikipedia.org)
  • While failing to show demonstrable disulfide reductase activity, MerA instead was found to have the ability to reduce two biologically-relevant transition metal ions without the aid of an interacting protein partner: Hg2+ and Fe3+. (sun.ac.za)
  • found
  • YpdA was shown to be incapable of reducing any of the LMW thiol disulfides found in S. aureus, even in the presence of a generic TFP protein, TrxA. (sun.ac.za)
  • sites
  • While the glutaredoxins and thioredoxins exhibit similar three-dimensional structures and active sites, the sources of electrons required for maintaining their reductive activities differ. (pnas.org)
  • include
  • The structural genes under this regulon include sodA (Mn-superoxide dismutase(SOD)), zwf (glucose-6-phosphate dehydrogenase(G6PDH)), acnA (aconitase A), nfsA (nitrate reductase A), fumC (fumarase C) and nfo (endonuclease IV) among others. (wikipedia.org)